                         SEQUENCE LISTING

<110>  THE CLEVELAND CLINIC FOUNDATION
 
<120>  TREATING DISEASE AND PROMOTING WEIGHT LOSS BY INHIBITING THE 
       TMA/FMO3/TMAO PATHWAY

<130>  CCF-35298/WO-1/ORD

<150>  US 62/521,872
<151>  2017-06-19

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  188
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  1
gaccatatag aagagggcag ggccagcatt taccaatcgg tcttcaccaa ctcttccaaa       60

gagatgatgt gtttccagac ttcccctatc ccgatgactt tcccaacttc atgcatcaca      120

gcaagctcca agaatacatc acttcatttg ccaaggaaaa gaacctcctg aaatacatac      180

agtttgag                                                               188


<210>  2
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  2
tgggaaagtc atcgggatag                                                   20


<210>  3
<211>  1125
<212>  DNA
<213>  Escherichia coli str. K-12 substr. DH10B

<400>  3
atgagcaatc tgagccctga ctttgtacta cccgaaaatt tttgcgctaa cccgcaagag       60

gcgtggacca ttcctgcccg tttttatacc gatcagaacg cgtttgaaca cgaaaaagag      120

aacgtcttcg ccaaaagctg gatttgcgtc gctcacagca gcgaactggc gaatgccaat      180

gattatgtga cgcgtgagat cattggcgaa agcatcgtgc tggtacgcgg tcgtgataag      240

gttttgcgcg cgttctataa cgtgtgtccg caccgtggtc atcagttgtt gagcggtgaa      300

ggaaaagcaa aaaatgtgat tacctgcccg tatcacgcat gggcattcaa actcgatggc      360

aacctggccc atgcacgtaa ctgcgaaaac gtcgccaatt tcgatagcga caaagcgcaa      420

ctggttccgg tgcgtctgga agaatatgcc ggattcgtct tcatcaacat ggaccccaac      480

gccaccagcg tagaagatca attacccggc ctgggcgcga aagtgctgga agcctgcccg      540

gaagtccacg atctgaaact ggcggcccgc tttaccaccc gcacgcctgc caactggaag      600

aacattgtcg ataactatct cgagtgctat cactgtggtc cggcgcatcc aggtttctcc      660

gactccgtac aggttgatcg ttactggcac accatgcacg gtaactggac gctgcaatac      720

ggtttcgcca aaccgtccga acagtcgttt aaatttgaag agggtacgga tgcggcattc      780

cacggtttct ggctgtggcc gtgcacgatg ctgaacgtca ccccgatcaa agggatgatg      840

acggtcattt atgaattccc ggtggattct gaaactaccc tgcaaaacta cgatatttac      900

ttcaccaatg aagagttaac cgacgagcaa aaatcgctga ttgagtggta tcgcgatgtg      960

ttccgtccgg aagatttacg tctggttgaa agcgtacaga aagggctgaa atcgcgtggc     1020

tatcgtggtc aggggcgcat catggccgac agtagcggta gtggcatttc cgaacatggt     1080

atcgcccatt tccataatct gctggcgcag gtgtttaagg actaa                     1125


<210>  4
<211>  374
<212>  PRT
<213>  Escherichia coli str. K-12 substr. DH10B

<400>  4

Met Ser Asn Leu Ser Pro Asp Phe Val Leu Pro Glu Asn Phe Cys Ala 
1               5                   10                  15      


Asn Pro Gln Glu Ala Trp Thr Ile Pro Ala Arg Phe Tyr Thr Asp Gln 
            20                  25                  30          


Asn Ala Phe Glu His Glu Lys Glu Asn Val Phe Ala Lys Ser Trp Ile 
        35                  40                  45              


Cys Val Ala His Ser Ser Glu Leu Ala Asn Ala Asn Asp Tyr Val Thr 
    50                  55                  60                  


Arg Glu Ile Ile Gly Glu Ser Ile Val Leu Val Arg Gly Arg Asp Lys 
65                  70                  75                  80  


Val Leu Arg Ala Phe Tyr Asn Val Cys Pro His Arg Gly His Gln Leu 
                85                  90                  95      


Leu Ser Gly Glu Gly Lys Ala Lys Asn Val Ile Thr Cys Pro Tyr His 
            100                 105                 110         


Ala Trp Ala Phe Lys Leu Asp Gly Asn Leu Ala His Ala Arg Asn Cys 
        115                 120                 125             


Glu Asn Val Ala Asn Phe Asp Ser Asp Lys Ala Gln Leu Val Pro Val 
    130                 135                 140                 


Arg Leu Glu Glu Tyr Ala Gly Phe Val Phe Ile Asn Met Asp Pro Asn 
145                 150                 155                 160 


Ala Thr Ser Val Glu Asp Gln Leu Pro Gly Leu Gly Ala Lys Val Leu 
                165                 170                 175     


Glu Ala Cys Pro Glu Val His Asp Leu Lys Leu Ala Ala Arg Phe Thr 
            180                 185                 190         


Thr Arg Thr Pro Ala Asn Trp Lys Asn Ile Val Asp Asn Tyr Leu Glu 
        195                 200                 205             


Cys Tyr His Cys Gly Pro Ala His Pro Gly Phe Ser Asp Ser Val Gln 
    210                 215                 220                 


Val Asp Arg Tyr Trp His Thr Met His Gly Asn Trp Thr Leu Gln Tyr 
225                 230                 235                 240 


Gly Phe Ala Lys Pro Ser Glu Gln Ser Phe Lys Phe Glu Glu Gly Thr 
                245                 250                 255     


Asp Ala Ala Phe His Gly Phe Trp Leu Trp Pro Cys Thr Met Leu Asn 
            260                 265                 270         


Val Thr Pro Ile Lys Gly Met Met Thr Val Ile Tyr Glu Phe Pro Val 
        275                 280                 285             


Asp Ser Glu Thr Thr Leu Gln Asn Tyr Asp Ile Tyr Phe Thr Asn Glu 
    290                 295                 300                 


Glu Leu Thr Asp Glu Gln Lys Ser Leu Ile Glu Trp Tyr Arg Asp Val 
305                 310                 315                 320 


Phe Arg Pro Glu Asp Leu Arg Leu Val Glu Ser Val Gln Lys Gly Leu 
                325                 330                 335     


Lys Ser Arg Gly Tyr Arg Gly Gln Gly Arg Ile Met Ala Asp Ser Ser 
            340                 345                 350         


Gly Ser Gly Ile Ser Glu His Gly Ile Ala His Phe His Asn Leu Leu 
        355                 360                 365             


Ala Gln Val Phe Lys Asp 
    370                 


<210>  5
<211>  966
<212>  DNA
<213>  Escherichia coli str. K-12 substr. DH10B

<400>  5
atgtcagact atcaaatgtt tgaagtacag gtgagccagg ttgaacccct taccgaacag       60

gtgaaacgct tcacgctggt ggcaaccgat ggcaaaccat tacctgcgtt taccggagga      120

agtcacgtca ttgtgcagat gagcgatggt gataaccagt acagcaatgc gtattcacta      180

ctgagttcgc cgcatgacac ctcttgttat cagattgccg ttcggctgga ggaaaactcg      240

cgcggcggtt cccgcttttt gcatcagcag gtaaaagtgg gcgatcggtt aacgatttca      300

acgcctaata acctgtttgc gctaattccc tcagccagaa agcatctgtt tatcgcgggc      360

ggtattggta tcaccccttt cctgtcgcac atggcagagc tgcaacacag cgacgtcgac      420

tggcagctac attactgctc gcgaaatcca gaaagttgcg catttcgtga tgagctagtc      480

cagcatccgc aggctgagaa agtccatttg catcattcat caaccggaac acgactggaa      540

ttagcgcgat tattggcgga tatcgaacct ggcacacacg tttatacctg tggccccgag      600

gcgctaattg aagcggtaag aagtgaagct gcgcgtctgg acatcgccgc cgatacgctg      660

cactttgagc aatttgctat cgaagacaaa accggcgatg catttaccct ggtgcttgcc      720

cgttccggaa aagagtttgt ggtgccggaa gagatgacta ttttgcaggt tattgaaaat      780

aataaagccg cgaaagtgga atgtttatgt cgtgaagggg tatgcggaac ctgcgaaaca      840

gcaatactgg aaggtgaagc tgaccatcgg gatcaatatt ttagcgatga agagcgtgcc      900

agccagcaaa gtatgttgat ctgttgttcg cgtgcgaagg gtaaacgcct ggtgttggat      960

ttgtag                                                                 966


<210>  6
<211>  321
<212>  PRT
<213>  Escherichia coli str. K-12 substr. DH10B

<400>  6

Met Ser Asp Tyr Gln Met Phe Glu Val Gln Val Ser Gln Val Glu Pro 
1               5                   10                  15      


Leu Thr Glu Gln Val Lys Arg Phe Thr Leu Val Ala Thr Asp Gly Lys 
            20                  25                  30          


Pro Leu Pro Ala Phe Thr Gly Gly Ser His Val Ile Val Gln Met Ser 
        35                  40                  45              


Asp Gly Asp Asn Gln Tyr Ser Asn Ala Tyr Ser Leu Leu Ser Ser Pro 
    50                  55                  60                  


His Asp Thr Ser Cys Tyr Gln Ile Ala Val Arg Leu Glu Glu Asn Ser 
65                  70                  75                  80  


Arg Gly Gly Ser Arg Phe Leu His Gln Gln Val Lys Val Gly Asp Arg 
                85                  90                  95      


Leu Thr Ile Ser Thr Pro Asn Asn Leu Phe Ala Leu Ile Pro Ser Ala 
            100                 105                 110         


Arg Lys His Leu Phe Ile Ala Gly Gly Ile Gly Ile Thr Pro Phe Leu 
        115                 120                 125             


Ser His Met Ala Glu Leu Gln His Ser Asp Val Asp Trp Gln Leu His 
    130                 135                 140                 


Tyr Cys Ser Arg Asn Pro Glu Ser Cys Ala Phe Arg Asp Glu Leu Val 
145                 150                 155                 160 


Gln His Pro Gln Ala Glu Lys Val His Leu His His Ser Ser Thr Gly 
                165                 170                 175     


Thr Arg Leu Glu Leu Ala Arg Leu Leu Ala Asp Ile Glu Pro Gly Thr 
            180                 185                 190         


His Val Tyr Thr Cys Gly Pro Glu Ala Leu Ile Glu Ala Val Arg Ser 
        195                 200                 205             


Glu Ala Ala Arg Leu Asp Ile Ala Ala Asp Thr Leu His Phe Glu Gln 
    210                 215                 220                 


Phe Ala Ile Glu Asp Lys Thr Gly Asp Ala Phe Thr Leu Val Leu Ala 
225                 230                 235                 240 


Arg Ser Gly Lys Glu Phe Val Val Pro Glu Glu Met Thr Ile Leu Gln 
                245                 250                 255     


Val Ile Glu Asn Asn Lys Ala Ala Lys Val Glu Cys Leu Cys Arg Glu 
            260                 265                 270         


Gly Val Cys Gly Thr Cys Glu Thr Ala Ile Leu Glu Gly Glu Ala Asp 
        275                 280                 285             


His Arg Asp Gln Tyr Phe Ser Asp Glu Glu Arg Ala Ser Gln Gln Ser 
    290                 295                 300                 


Met Leu Ile Cys Cys Ser Arg Ala Lys Gly Lys Arg Leu Val Leu Asp 
305                 310                 315                 320 


Leu 
    


<210>  7
<211>  50
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  7

Thr Thr Leu Ser Lys Ser Leu Ala Gly Ala Ala Lys His Glu Arg Lys 
1               5                   10                  15      


Ala Ala Lys Thr Leu Gly Ile Ala Val Gly Ile Tyr Leu Leu Cys Trp 
            20                  25                  30          


Leu Pro Phe Thr Ile Asp Thr Met Val Asp Ser Leu Leu His Phe Ile 
        35                  40                  45              


Thr Pro 
    50  


