                         
							    PCT/EP2018/066 668 - 16-08-2018


					SEQUENCE LISTING

<110>  TRANSGENE SA
 
<120>  Personalized vaccine

<130>  B374744PCTD38479

<150>  EP 17305760.5
<151>  2017-06-21

<150>  EP 18305496.4
<151>  2018-04-23

<160>  70    

<170>  PatentIn version 3.5

<210>  1
<211>  27
<212>  PRT
<213>  artificial sequence

<220>
<223>  FCU1 peptide

<400>  1

Cys Ser Lys Glu Gly Ile Glu Lys Tyr His Ala Ala Phe Pro Glu Val 
1               5                   10                  15      


Arg Ile Val Thr Gly Ala Leu Asp Arg Gly Leu 
            20                  25          


<210>  2
<211>  27
<212>  PRT
<213>  artificial sequence

<220>
<223>  peptide obtained from beta galatosidase antigen

<400>  2

Gly Gly Ala Asp Thr Thr Ala Thr Asp Ile Ile Cys Pro Met Tyr Ala 
1               5                   10                  15      


Arg Val Asp Glu Asp Gln Pro Phe Pro Ala Val 
            20                  25          


<210>  3
<211>  27
<212>  PRT
<213>  artificial sequence

<220>
<223>  peptide obtained from HPV-16 E1 antigen

<400>  3

Tyr Asp Asn Asp Ile Val Asp Asp Ser Glu Ile Ala Tyr Lys Tyr Ala 
1               5                   10                  15      


Gln Leu Ala Asp Thr Asn Ser Asn Ala Ser Ala 
            20                  25          


<210>  4
<211>  27
<212>  PRT
<213>  artificial sequence

<220>
<223>  peptide obtained from HPV-16 E7 antigen

<400>  4

Gly Pro Ala Gly Gln Ala Glu Pro Asp Arg Ala His Tyr Asn Ile Val 
1               5                   10                  15      


Thr Phe Cys Cys Lys Cys Asp Ser Thr Leu Arg 
            20                  25          


<210>  5
<211>  27
<212>  PRT
<213>  artificial sequence

<220>
<223>  peptide obtained from MUC1 antigen

<400>  5

Tyr Glu Lys Val Ser Ala Gly Asn Gly Gly Ser Ser Leu Ser Tyr Thr 
1               5                   10                  15      


Asn Pro Ala Val Ala Ala Thr Ser Ala Asn Leu 
            20                  25          


<210>  6
<211>  8
<212>  PRT
<213>  artificial sequence

<220>
<223>  Flag tag

<400>  6

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  7
<211>  204
<212>  PRT
<213>  artificial sequence

<220>
<223>  fusion of 5 peptides expressed by MVATG19022

<400>  7

Met Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Cys Ser Lys Glu Gly 
1               5                   10                  15      


Ile Glu Lys Tyr His Ala Ala Phe Pro Glu Val Arg Ile Val Thr Gly 
            20                  25                  30          


Ala Leu Asp Arg Gly Leu Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser 
        35                  40                  45              


Gly Gly Ala Asp Thr Thr Ala Thr Asp Ile Ile Cys Pro Met Tyr Ala 
    50                  55                  60                  


Arg Val Asp Glu Asp Gln Pro Phe Pro Ala Val Gly Ser Gly Ser Gly 
65                  70                  75                  80  


Ser Gly Ser Gly Ser Tyr Asp Asn Asp Ile Val Asp Asp Ser Glu Ile 
                85                  90                  95      


Ala Tyr Lys Tyr Ala Gln Leu Ala Asp Thr Asn Ser Asn Ala Ser Ala 
            100                 105                 110         


Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Pro Ala Gly Gln Ala 
        115                 120                 125             


Glu Pro Asp Arg Ala His Tyr Asn Ile Val Thr Phe Cys Cys Lys Cys 
    130                 135                 140                 


Asp Ser Thr Leu Arg Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Tyr 
145                 150                 155                 160 


Glu Lys Val Ser Ala Gly Asn Gly Gly Ser Ser Leu Ser Tyr Thr Asn 
                165                 170                 175     


Pro Ala Val Ala Ala Thr Ser Ala Asn Leu Gly Ser Gly Ser Gly Ser 
            180                 185                 190         


Gly Ser Gly Ser Asp Tyr Lys Asp Asp Asp Asp Lys 
        195                 200                 


<210>  8
<211>  112
<212>  DNA
<213>  artificial sequence

<220>
<223>  fragment of vaccinia promoter p11k7.5

<400>  8
ataaaaatat agtagaattt cattttgttt ttttctatgc tataaatagg atccgataaa       60

gtgaaaaata attctaattt attgcacggt aaggaagtag aatcataaag aa              112


<210>  9
<211>  23
<212>  PRT
<213>  artificial sequence

<220>
<223>  fragment of rabbies glycoprotein (signal peptide)

<400>  9

Met Val Pro Gln Ala Leu Leu Phe Val Pro Leu Leu Val Phe Pro Leu 
1               5                   10                  15      


Cys Phe Gly Lys Phe Pro Ile 
            20              


<210>  10
<211>  226
<212>  PRT
<213>  artificial sequence

<220>
<223>  fusion of 5 peptides encoded by MVATG19023 (+ signal peptide)

<400>  10

Met Val Pro Gln Ala Leu Leu Phe Val Pro Leu Leu Val Phe Pro Leu 
1               5                   10                  15      


Cys Phe Gly Lys Phe Pro Ile Gly Ser Gly Ser Gly Ser Gly Ser Gly 
            20                  25                  30          


Ser Cys Ser Lys Glu Gly Ile Glu Lys Tyr His Ala Ala Phe Pro Glu 
        35                  40                  45              


Val Arg Ile Val Thr Gly Ala Leu Asp Arg Gly Leu Gly Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Ser Gly Gly Ala Asp Thr Thr Ala Thr Asp Ile 
65                  70                  75                  80  


Ile Cys Pro Met Tyr Ala Arg Val Asp Glu Asp Gln Pro Phe Pro Ala 
                85                  90                  95      


Val Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Tyr Asp Asn Asp Ile 
            100                 105                 110         


Val Asp Asp Ser Glu Ile Ala Tyr Lys Tyr Ala Gln Leu Ala Asp Thr 
        115                 120                 125             


Asn Ser Asn Ala Ser Ala Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser 
    130                 135                 140                 


Gly Pro Ala Gly Gln Ala Glu Pro Asp Arg Ala His Tyr Asn Ile Val 
145                 150                 155                 160 


Thr Phe Cys Cys Lys Cys Asp Ser Thr Leu Arg Gly Ser Gly Ser Gly 
                165                 170                 175     


Ser Gly Ser Gly Ser Tyr Glu Lys Val Ser Ala Gly Asn Gly Gly Ser 
            180                 185                 190         


Ser Leu Ser Tyr Thr Asn Pro Ala Val Ala Ala Thr Ser Ala Asn Leu 
        195                 200                 205             


Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Asp Tyr Lys Asp Asp Asp 
    210                 215                 220                 


Asp Lys 
225     


<210>  11
<211>  10
<212>  PRT
<213>  artificial sequence

<220>
<223>  cMYC tag

<400>  11

Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
1               5                   10  


<210>  12
<211>  204
<212>  PRT
<213>  artificial sequence

<220>
<223>  fusion of 5 CT26 neopeptides (pentatope 1)

<400>  12

Met Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Tyr Arg Gly Ala Asn 
1               5                   10                  15      


Leu His Leu Glu Glu Thr Leu Ala Gly Phe Trp Ala Arg Leu Leu Glu 
            20                  25                  30          


Arg Leu Phe Lys Gln Leu Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser 
        35                  40                  45              


Gly Tyr Ile Ser Arg Val Thr Ala Gly Lys Asp Ser Tyr Ile Ala Leu 
    50                  55                  60                  


Val Asp Lys Asn Ile Met Gly Tyr Ile Ala Ser Gly Ser Gly Ser Gly 
65                  70                  75                  80  


Ser Gly Ser Gly Ser Ala Gly Thr Gln Cys Glu Tyr Trp Ala Ser Arg 
                85                  90                  95      


Ala Leu Asp Ser Glu His Ser Ile Gly Ser Met Ile Gln Leu Pro Gln 
            100                 105                 110         


Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Glu Gly Asp Pro Cys Leu 
        115                 120                 125             


Arg Ser Ser Asp Cys Ile Asp Glu Phe Cys Cys Ala Arg His Phe Trp 
    130                 135                 140                 


Thr Lys Ile Cys Lys Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Val 
145                 150                 155                 160 


Thr Ser Ile Pro Ser Val Ser Asn Ala Leu Asn Trp Lys Glu Phe Ser 
                165                 170                 175     


Phe Ile Gln Ser Thr Leu Gly Tyr Val Ala Gly Ser Gly Ser Gly Ser 
            180                 185                 190         


Gly Ser Gly Ser Asp Tyr Lys Asp Asp Asp Asp Lys 
        195                 200                 


<210>  13
<211>  206
<212>  PRT
<213>  artificial sequence

<220>
<223>  fusion of 5 CT26 neopeptides (pentatope 2) encoded by MVATG19030

<400>  13

Met Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Pro Leu Leu Pro Phe 
1               5                   10                  15      


Tyr Pro Pro Asp Glu Ala Leu Glu Ile Gly Leu Glu Leu Asn Ser Ser 
            20                  25                  30          


Ala Leu Pro Pro Thr Glu Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser 
        35                  40                  45              


Val Ile Leu Pro Gln Ala Pro Ser Gly Pro Ser Tyr Ala Thr Tyr Leu 
    50                  55                  60                  


Gln Pro Ala Gln Ala Gln Met Leu Thr Pro Pro Gly Ser Gly Ser Gly 
65                  70                  75                  80  


Ser Gly Ser Gly Ser Asp Lys Pro Leu Arg Arg Asn Asn Ser Tyr Thr 
                85                  90                  95      


Ser Tyr Ile Met Ala Ile Cys Gly Met Pro Leu Asp Ser Phe Arg Ala 
            100                 105                 110         


Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Glu Val Ile Gln Thr Ser 
        115                 120                 125             


Lys Tyr Tyr Met Arg Asp Val Ile Ala Ile Glu Ser Ala Trp Leu Leu 
    130                 135                 140                 


Glu Leu Ala Pro His Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Glu 
145                 150                 155                 160 


His Ile His Arg Ala Gly Gly Leu Phe Val Ala Asp Ala Ile Gln Val 
                165                 170                 175     


Gly Phe Gly Arg Ile Gly Lys His Phe Trp Gly Ser Gly Ser Gly Ser 
            180                 185                 190         


Gly Ser Gly Ser Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
        195                 200                 205     


<210>  14
<211>  114
<212>  DNA
<213>  artificial sequence

<220>
<223>  fragment of vaccinia promoter pH5R

<400>  14
tttattctat acttaaaaaa tgaaaataaa tacaaaggtt cttgagggtt gtgttaaatt       60

gaaagcgaga aataatcata aattatttca ttatcgcgat atccgttaag tttg            114


<210>  15
<211>  28
<212>  PRT
<213>  artificial sequence

<220>
<223>  fragment of measles F glycoprotein (signal peptide)

<400>  15

Met Gly Leu Lys Val Asn Val Ser Ala Ile Phe Met Ala Val Leu Leu 
1               5                   10                  15      


Thr Leu Gln Thr Pro Thr Gly Gln Ile His Trp Gly 
            20                  25              


<210>  16
<211>  226
<212>  PRT
<213>  artificial sequence

<220>
<223>  fusion of 5 CT26 neopeptides encoded by MVATG19038 (pentatope 1)

<400>  16

Met Val Pro Gln Ala Leu Leu Phe Val Pro Leu Leu Val Phe Pro Leu 
1               5                   10                  15      


Cys Phe Gly Lys Phe Pro Ile Gly Ser Gly Ser Gly Ser Gly Ser Gly 
            20                  25                  30          


Ser Tyr Arg Gly Ala Asn Leu His Leu Glu Glu Thr Leu Ala Gly Phe 
        35                  40                  45              


Trp Ala Arg Leu Leu Glu Arg Leu Phe Lys Gln Leu Gly Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Ser Gly Tyr Ile Ser Arg Val Thr Ala Gly Lys 
65                  70                  75                  80  


Asp Ser Tyr Ile Ala Leu Val Asp Lys Asn Ile Met Gly Tyr Ile Ala 
                85                  90                  95      


Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Ala Gly Thr Gln Cys 
            100                 105                 110         


Glu Tyr Trp Ala Ser Arg Ala Leu Asp Ser Glu His Ser Ile Gly Ser 
        115                 120                 125             


Met Ile Gln Leu Pro Gln Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser 
    130                 135                 140                 


Glu Gly Asp Pro Cys Leu Arg Ser Ser Asp Cys Ile Asp Glu Phe Cys 
145                 150                 155                 160 


Cys Ala Arg His Phe Trp Thr Lys Ile Cys Lys Gly Ser Gly Ser Gly 
                165                 170                 175     


Ser Gly Ser Gly Ser Val Thr Ser Ile Pro Ser Val Ser Asn Ala Leu 
            180                 185                 190         


Asn Trp Lys Glu Phe Ser Phe Ile Gln Ser Thr Leu Gly Tyr Val Ala 
        195                 200                 205             


Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Asp Tyr Lys Asp Asp Asp 
    210                 215                 220                 


Asp Lys 
225     


<210>  17
<211>  232
<212>  PRT
<213>  artificial sequence

<220>
<223>  fusion of 5 CT26 neopeptides encoded by MVATG19038 (pentatope 2)

<400>  17

Met Gly Leu Lys Val Asn Val Ser Ala Ile Phe Met Ala Val Leu Leu 
1               5                   10                  15      


Thr Leu Gln Thr Pro Thr Gly Gln Ile His Trp Gly Ser Gly Ser Gly 
            20                  25                  30          


Ser Gly Ser Gly Ser Pro Leu Leu Pro Phe Tyr Pro Pro Asp Glu Ala 
        35                  40                  45              


Leu Glu Ile Gly Leu Glu Leu Asn Ser Ser Ala Leu Pro Pro Thr Glu 
    50                  55                  60                  


Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Val Ile Leu Pro Gln Ala 
65                  70                  75                  80  


Pro Ser Gly Pro Ser Tyr Ala Thr Tyr Leu Gln Pro Ala Gln Ala Gln 
                85                  90                  95      


Met Leu Thr Pro Pro Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Asp 
            100                 105                 110         


Lys Pro Leu Arg Arg Asn Asn Ser Tyr Thr Ser Tyr Ile Met Ala Ile 
        115                 120                 125             


Cys Gly Met Pro Leu Asp Ser Phe Arg Ala Gly Ser Gly Ser Gly Ser 
    130                 135                 140                 


Gly Ser Gly Ser Glu Val Ile Gln Thr Ser Lys Tyr Tyr Met Arg Asp 
145                 150                 155                 160 


Val Ile Ala Ile Glu Ser Ala Trp Leu Leu Glu Leu Ala Pro His Gly 
                165                 170                 175     


Ser Gly Ser Gly Ser Gly Ser Gly Ser Glu His Ile His Arg Ala Gly 
            180                 185                 190         


Gly Leu Phe Val Ala Asp Ala Ile Gln Val Gly Phe Gly Arg Ile Gly 
        195                 200                 205             


Lys His Phe Trp Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Glu Gln 
    210                 215                 220                 


Lys Leu Ile Ser Glu Glu Asp Leu 
225                 230         


<210>  18
<211>  9
<212>  PRT
<213>  artificial sequence

<220>
<223>  HA tag

<400>  18

Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   


<210>  19
<211>  11
<212>  PRT
<213>  artificial sequence

<220>
<223>  HSV tag

<400>  19

Gln Pro Glu Leu Ala Pro Glu Asp Pro Glu Asp 
1               5                   10      


<210>  20
<211>  11
<212>  PRT
<213>  artificial sequence

<220>
<223>  VSV tag

<400>  20

Tyr Thr Asp Ile Glu Met Asn Arg Leu Gly Lys 
1               5                   10      


<210>  21
<211>  100
<212>  DNA
<213>  artificial sequence

<220>
<223>  fragment of vaccinia pB2R promoter

<400>  21
tatattatta agtgtggtgt ttggtcgatg taaaattttt gtcgataaaa attaaaaaat       60

aacttaattt attattgatc tcgtgtgtac aaccgaaatc                            100


<210>  22
<211>  27
<212>  PRT
<213>  artificial sequence

<220>
<223>  CT26 peptide 1

<400>  22

Tyr Arg Gly Ala Asn Leu His Leu Glu Glu Thr Leu Ala Gly Phe Trp 
1               5                   10                  15      


Ala Arg Leu Leu Glu Arg Leu Phe Lys Gln Leu 
            20                  25          


<210>  23
<211>  27
<212>  PRT
<213>  artificial sequence

<220>
<223>  CT26 peptide 2

<400>  23

Gly Tyr Ile Ser Arg Val Thr Ala Gly Lys Asp Ser Tyr Ile Ala Leu 
1               5                   10                  15      


Val Asp Lys Asn Ile Met Gly Tyr Ile Ala Ser 
            20                  25          


<210>  24
<211>  27
<212>  PRT
<213>  artificial sequence

<220>
<223>  CT26 peptide 3

<400>  24

Ala Gly Thr Gln Cys Glu Tyr Trp Ala Ser Arg Ala Leu Asp Ser Glu 
1               5                   10                  15      


His Ser Ile Gly Ser Met Ile Gln Leu Pro Gln 
            20                  25          


<210>  25
<211>  27
<212>  PRT
<213>  artificial sequence

<220>
<223>  CT26 neopeptide 4

<400>  25

Glu Gly Asp Pro Cys Leu Arg Ser Ser Asp Cys Ile Asp Glu Phe Cys 
1               5                   10                  15      


Cys Ala Arg His Phe Trp Thr Lys Ile Cys Lys 
            20                  25          


<210>  26
<211>  27
<212>  PRT
<213>  artificial sequence

<220>
<223>  CT26 neopeptide 5

<400>  26

Val Thr Ser Ile Pro Ser Val Ser Asn Ala Leu Asn Trp Lys Glu Phe 
1               5                   10                  15      


Ser Phe Ile Gln Ser Thr Leu Gly Tyr Val Ala 
            20                  25          


<210>  27
<211>  27
<212>  PRT
<213>  artificial sequence

<220>
<223>  CT26 neopeptide 6

<400>  27

Pro Leu Leu Pro Phe Tyr Pro Pro Asp Glu Ala Leu Glu Ile Gly Leu 
1               5                   10                  15      


Glu Leu Asn Ser Ser Ala Leu Pro Pro Thr Glu 
            20                  25          


<210>  28
<211>  27
<212>  PRT
<213>  artificial sequence

<220>
<223>  CT26 neopeptide 7

<400>  28

Val Ile Leu Pro Gln Ala Pro Ser Gly Pro Ser Tyr Ala Thr Tyr Leu 
1               5                   10                  15      


Gln Pro Ala Gln Ala Gln Met Leu Thr Pro Pro 
            20                  25          


<210>  29
<211>  27
<212>  PRT
<213>  artificial sequence

<220>
<223>  CT26 neopeptide 8

<400>  29

Asp Lys Pro Leu Arg Arg Asn Asn Ser Tyr Thr Ser Tyr Ile Met Ala 
1               5                   10                  15      


Ile Cys Gly Met Pro Leu Asp Ser Phe Arg Ala 
            20                  25          


<210>  30
<211>  27
<212>  PRT
<213>  artificial sequence

<220>
<223>  CT26 neopeptide 9

<400>  30

Glu Val Ile Gln Thr Ser Lys Tyr Tyr Met Arg Asp Val Ile Ala Ile 
1               5                   10                  15      


Glu Ser Ala Trp Leu Leu Glu Leu Ala Pro His 
            20                  25          


<210>  31
<211>  27
<212>  PRT
<213>  artificial sequence

<220>
<223>  CT26 neopeptide 10

<400>  31

Glu His Ile His Arg Ala Gly Gly Leu Phe Val Ala Asp Ala Ile Gln 
1               5                   10                  15      


Val Gly Phe Gly Arg Ile Gly Lys His Phe Trp 
            20                  25          


<210>  32
<211>  27
<212>  PRT
<213>  artificial sequence

<220>
<223>  non mutated CT26 peptide 1

<400>  32

Tyr Arg Gly Ala Asn Leu His Leu Glu Glu Thr Leu Ala Glu Phe Trp 
1               5                   10                  15      


Ala Arg Leu Leu Glu Arg Leu Phe Lys Gln Leu 
            20                  25          


<210>  33
<211>  27
<212>  PRT
<213>  artificial sequence

<220>
<223>  non mutated CT26 peptide 2

<400>  33

Gly Tyr Ile Ser Arg Val Thr Ala Gly Lys Asp Ser Tyr Leu Ala Leu 
1               5                   10                  15      


Val Asp Lys Asn Ile Met Gly Tyr Ile Ala Ser 
            20                  25          


<210>  34
<211>  27
<212>  PRT
<213>  artificial sequence

<220>
<223>  non mutated CT26 peptide 3

<400>  34

Ala Gly Thr Gln Cys Glu Tyr Trp Ala Ser Arg Ala Leu Gly Ser Glu 
1               5                   10                  15      


His Ser Ile Gly Ser Met Ile Gln Leu Pro Gln 
            20                  25          


<210>  35
<211>  27
<212>  PRT
<213>  artificial sequence

<220>
<223>  non mutated CT26 peptide 4

<400>  35

Glu Gly Asp Pro Cys Leu Arg Ser Ser Asp Cys Ile Asp Gly Phe Cys 
1               5                   10                  15      


Cys Ala Arg His Phe Trp Thr Lys Ile Cys Lys 
            20                  25          


<210>  36
<211>  27
<212>  PRT
<213>  artificial sequence

<220>
<223>  non mutated CT26 peptide 5

<400>  36

Val Thr Ser Ile Pro Ser Val Ser Asn Ala Leu Asn Trp Arg Glu Phe 
1               5                   10                  15      


Ser Phe Ile Gln Ser Thr Leu Gly Tyr Val Ala 
            20                  25          


<210>  37
<211>  27
<212>  PRT
<213>  artificial sequence

<220>
<223>  non mutated CT26 peptide 6

<400>  37

Pro Leu Leu Pro Phe Tyr Pro Pro Asp Glu Ala Leu Glu Thr Gly Leu 
1               5                   10                  15      


Glu Leu Asn Ser Ser Ala Leu Pro Pro Thr Glu 
            20                  25          


<210>  38
<211>  27
<212>  PRT
<213>  artificial sequence

<220>
<223>  non mutated CT26 peptide 7

<400>  38

Val Ile Leu Pro Gln Ala Pro Ser Gly Pro Ser Tyr Ala Ile Tyr Leu 
1               5                   10                  15      


Gln Pro Ala Gln Ala Gln Met Leu Thr Pro Pro 
            20                  25          


<210>  39
<211>  27
<212>  PRT
<213>  artificial sequence

<220>
<223>  non mutated CT26 peptide 8

<400>  39

Asp Lys Pro Leu Arg Arg Asn Asn Ser Tyr Thr Ser Tyr Thr Met Ala 
1               5                   10                  15      


Ile Cys Gly Met Pro Leu Asp Ser Phe Arg Ala 
            20                  25          


<210>  40
<211>  27
<212>  PRT
<213>  artificial sequence

<220>
<223>  non mutated CT26 peptide 9

<400>  40

Glu Val Ile Gln Thr Ser Lys Tyr Tyr Met Arg Asp Val Thr Ala Ile 
1               5                   10                  15      


Glu Ser Ala Trp Leu Leu Glu Leu Ala Pro His 
            20                  25          


<210>  41
<211>  27
<212>  PRT
<213>  artificial sequence

<220>
<223>  non mutated CT26 peptide 10

<400>  41

Glu His Ile His Arg Ala Gly Gly Leu Phe Val Ala Asp Glu Ile Gln 
1               5                   10                  15      


Val Gly Phe Gly Arg Ile Gly Lys His Phe Trp 
            20                  25          


<210>  42
<211>  9
<212>  PRT
<213>  artificial sequence

<220>
<223>  CD8 CT26 epitope 7M1

<400>  42

Pro Ser Tyr Ala Thr Tyr Leu Gln Pro 
1               5                   


<210>  43
<211>  9
<212>  PRT
<213>  artificial sequence

<220>
<223>  CD8 CT26 epitope 7M2

<400>  43

Tyr Ala Thr Tyr Leu Gln Pro Ala Gln 
1               5                   


<210>  44
<211>  9
<212>  PRT
<213>  artificial sequence

<220>
<223>  CD8 CT26 epitope 7M3

<400>  44

Thr Tyr Leu Gln Pro Ala Gln Ala Gln 
1               5                   


<210>  45
<211>  9
<212>  PRT
<213>  artificial sequence

<220>
<223>  CD8 CT26 epitope 2M

<400>  45

Ile Ala Leu Val Asp Lys Asn Ile Met 
1               5                   


<210>  46
<211>  9
<212>  PRT
<213>  artificial sequence

<220>
<223>  CD8 CT26 epitope 7WT1

<400>  46

Pro Ser Tyr Ala Ile Tyr Leu Gln Pro 
1               5                   


<210>  47
<211>  9
<212>  PRT
<213>  artificial sequence

<220>
<223>  CD8 CT26 epitope 7WT2

<400>  47

Tyr Ala Ile Tyr Leu Gln Pro Ala Gln 
1               5                   


<210>  48
<211>  9
<212>  PRT
<213>  artificial sequence

<220>
<223>  CD8 CT26 epitope 7WT3

<400>  48

Ile Tyr Leu Gln Pro Ala Gln Ala Gln 
1               5                   


<210>  49
<211>  9
<212>  PRT
<213>  artificial sequence

<220>
<223>  CD8 CT- epitope 2 WT

<400>  49

Leu Ala Leu Val Asp Lys Asn Ile Met 
1               5                   


<210>  50
<211>  8
<212>  PRT
<213>  artificial sequence

<220>
<223>  stimulating peptide

<400>  50

Ile Ala Tyr Lys Tyr Ala Gln Leu 
1               5               


<210>  51
<211>  8
<212>  PRT
<213>  artificial sequence

<220>
<223>  stimulating peptide

<400>  51

Ile Cys Pro Met Tyr Ala Arg Val 
1               5               


<210>  52
<211>  9
<212>  PRT
<213>  artificial sequence

<220>
<223>  stimulating peptide

<400>  52

Arg Ala His Tyr Asn Ile Val Thr Phe 
1               5                   


<210>  53
<211>  15
<212>  PRT
<213>  artificial sequence

<220>
<223>  stimulating peptide

<400>  53

Leu Ser Tyr Thr Asn Pro Ala Val Ala Ala Thr Ser Ala Asn Leu 
1               5                   10                  15  


<210>  54
<211>  15
<212>  PRT
<213>  artificial sequence

<220>
<223>  stimulating peptide

<400>  54

Glu Lys Tyr His Ala Ala Phe Pro Glu Val Arg Ile Val Thr Gly 
1               5                   10                  15  


<210>  55
<211>  8
<212>  PRT
<213>  artificial sequence

<220>
<223>  stimulating peptide

<400>  55

Thr Ser Tyr Lys Phe Glu Ser Val 
1               5               


<210>  56
<211>  9
<212>  PRT
<213>  artificial sequence

<220>
<223>  irrelevant peptide

<400>  56

Lys Asn Gly Glu Asn Ala Gln Ala Ile 
1               5                   


<210>  57
<211>  15
<212>  PRT
<213>  artificial sequence

<220>
<223>  irrelevant peptide

<400>  57

Gly Ile Cys Leu Met Leu Phe Ile Leu Ile Lys Arg Ser Arg His 
1               5                   10                  15  


<210>  58
<211>  27
<212>  PRT
<213>  artificial sequence

<220>
<223>  example of a lung adenocarcinoma neopeptide which dispays 
       negative hydrobobicity and hydropathy scores

<400>  58

Gly Gln Ser Leu Pro Met Thr His Ser Leu Lys Leu Ser Lys Thr Asn 
1               5                   10                  15      


Arg Thr Leu Phe Leu Leu Gly Val Thr Lys Tyr 
            20                  25          


<210>  59
<211>  27
<212>  PRT
<213>  artificial sequence

<220>
<223>  Example of a lung adenocarcinoma neopeptide which dispaly 
       positive hydrophobicity and hydropathy scores

<400>  59

Gly Leu Met Gly Ile Val Val Gly Thr Val Phe Ile Ile Arg Gly Leu 
1               5                   10                  15      


Arg Ser Val Gly Ala Ser Arg His Gln Gly Leu 
            20                  25          


<210>  60
<211>  270
<212>  PRT
<213>  artificial sequence

<220>
<223>  fusion 1 of ten neopeptides encoded by pTG19247

<400>  60

Lys Glu His Gly Thr His Gln Gln Leu Leu Ala Gln Lys Val Ile Tyr 
1               5                   10                  15      


Phe Ser Met Val Ser Val Gln Ala Gly Thr Lys Ala Trp Glu Ser Trp 
            20                  25                  30          


Arg Ser Glu Val Gly Lys Gln Leu Met Pro Leu Tyr Glu Glu Tyr Val 
        35                  40                  45              


Val Leu Lys Asn Glu Met Asp Val Ser Lys Glu Asn Glu Asn Ser Met 
    50                  55                  60                  


Val Pro Gln Arg Ala Pro Val Gly Ser Leu Ser Val Lys Asn Lys Ala 
65                  70                  75                  80  


His Gln Met Lys Ala Arg Leu Thr Gln Glu Leu Gln Gln Ala Asn Asn 
                85                  90                  95      


Met His Asn Val Leu Gln Ala Glu Leu Asp Lys Leu His Leu His Tyr 
            100                 105                 110         


Pro Ile Glu Arg Gly Leu Val Thr Gly Cys Asp Asp Met Glu Lys Leu 
        115                 120                 125             


Trp Lys His Leu Phe Glu Arg Ala Val Pro Tyr Met Ala Lys Phe Val 
    130                 135                 140                 


Ile Phe Ala Lys Ile Asn Asp Pro Arg Glu Gly Arg Leu Arg Cys Tyr 
145                 150                 155                 160 


Cys Met Lys Thr Val Val Val Met Leu Gln Asn Val Val Pro Ala Lys 
                165                 170                 175     


Val Ser Leu Val Cys Val Val Lys Pro Asp Glu Phe Trp Ser Gly Val 
            180                 185                 190         


Lys Val Ser Val Tyr Ala Val Pro Asp Lys Met Asn Gln Ala Asp Tyr 
        195                 200                 205             


Ala Leu Asp Ala Ala Val Thr Leu Trp Asn Asn Gly Arg Asn His Leu 
    210                 215                 220                 


Ile Phe Asn Leu Tyr Phe Gly Thr Trp Pro Asp Tyr Thr Glu Asp Val 
225                 230                 235                 240 


Gly Phe Asp Ile Arg Gln Pro Pro Gly Lys Ala Leu Glu Trp Leu Ala 
                245                 250                 255     


His Ile Tyr Trp Asp Asp Asp Lys Arg Tyr Ser Pro Ser Leu 
            260                 265                 270 


<210>  61
<211>  267
<212>  PRT
<213>  artificial sequence

<220>
<223>  fusion 2 of 10 neopeptides encoded by pTG19247

<400>  61

Gly Gln Ser Leu Pro Met Thr His Ser Leu Lys Leu Ser Lys Thr Asn 
1               5                   10                  15      


Arg Thr Leu Phe Leu Leu Gly Val Thr Lys Tyr Gly Leu Met Gly Ile 
            20                  25                  30          


Val Val Gly Thr Val Phe Ile Ile Arg Gly Leu Arg Ser Val Gly Ala 
        35                  40                  45              


Ser Arg His Gln Gly Leu Trp Tyr Val Arg Met Asn Arg Arg Arg Leu 
    50                  55                  60                  


Lys Gly Glu Thr Gly Met Glu Asp Cys Val Met Ala Leu Glu Thr Leu 
65                  70                  75                  80  


Phe Thr Ala Ile Pro Leu Glu Ile Phe Ile Val Thr Met Glu Asn Asp 
                85                  90                  95      


Phe Pro Gly Gly Val Ile Gly Lys Ile His Ala Thr Gly Arg Gly Arg 
            100                 105                 110         


Ser Lys Leu Lys Ser Gly Ile Gly Ala Leu Val Leu Pro Gly Val Ser 
        115                 120                 125             


Thr Ala Asp Ile Ser Ser Asn His Gln Arg Glu Asn Ser Gly His Arg 
    130                 135                 140                 


Arg Asp Gln Ile Thr Glu Lys Asp Ala Ala Leu Cys Val Leu Ile Asp 
145                 150                 155                 160 


Glu Met His Asn Tyr Met Tyr Trp Tyr Arg Gln Asp Pro Gly Met Val 
                165                 170                 175     


Leu Lys Leu Ile Tyr Tyr Ser Val Gly Ala Gly Ile Thr Gly Trp Met 
            180                 185                 190         


Tyr Gly Thr Val Gln Arg Thr Gly Arg Thr Arg Met Leu Pro Ala Asn 
        195                 200                 205             


Tyr Val Glu Ala Ile Glu Glu Trp Glu Cys Leu Asp Ser Ala Gln Gln 
    210                 215                 220                 


Arg Leu His Arg Asp Val Met Leu Glu Asn Tyr Gly Asn Leu Phe Ser 
225                 230                 235                 240 


Leu Phe Thr Val Ser Pro Thr Leu Tyr Phe Leu His Ser Val Ser Leu 
                245                 250                 255     


Pro Ala Leu Asp Leu Lys Pro Gly Glu Gly Ala 
            260                 265         


<210>  62
<211>  270
<212>  PRT
<213>  artificial sequence

<220>
<223>  fusion 3 of 10 neopeptides encoded by pTG19247

<400>  62

Val Val Gly Trp Arg Ser Gly Val Glu Lys Asp Leu Asp Lys Val Leu 
1               5                   10                  15      


Gln Thr His Ser Val Phe Val Asn Val Ser Lys Lys Gly Ile Arg Thr 
            20                  25                  30          


Leu Leu Phe Ala Leu Met Met Ser Phe Pro Ala Leu Phe Asn Ile Gly 
        35                  40                  45              


Leu Leu Leu Phe Leu Val Glu Ala Thr Asn Ala Ser Val Phe Lys Tyr 
    50                  55                  60                  


Tyr Ile His Gly Leu Ser Asp Leu Ile Asp Cys Cys Asp Leu Gly Tyr 
65                  70                  75                  80  


His Ser Ile Phe Tyr Leu Leu Lys Ile Ala Asn Phe Ser Asn Leu Ile 
                85                  90                  95      


Phe Leu His Leu Lys Arg Arg Val Lys Ser Val Ile Val His His Arg 
            100                 105                 110         


Ile Val Gly Cys Ser Leu Ala Val Ile Cys Gly Val Leu Tyr Gly Ser 
        115                 120                 125             


Thr Phe Val Pro Ile Ile Tyr Phe Leu Val Ile Ala Leu Lys Asn Ala 
    130                 135                 140                 


Val Glu Ile Tyr Val Trp Ala Pro Lys Pro Tyr His Lys Phe Met Ala 
145                 150                 155                 160 


Phe Lys Pro Gly Leu Asp Arg Glu Gln Leu Tyr Trp Glu Leu Ser Lys 
                165                 170                 175     


Leu Thr His Ser Ile Thr Glu Leu Gly Pro Tyr Thr Leu Leu Leu Gln 
            180                 185                 190         


Ile Ser Asn Trp Phe Ile Asn Ala Arg Arg Cys Ile Leu Pro Asp Met 
        195                 200                 205             


Leu Gln Gln Arg Arg Asn Asp Pro Thr Ile Ser Leu Ser Gly Cys Ala 
    210                 215                 220                 


Val Gln Met Phe Leu Ser Leu Ala Met Gly Thr Thr Glu Cys Val Leu 
225                 230                 235                 240 


Leu Gly Met Gln Ile Leu Ser Lys Leu Leu Leu Asp Leu Ser Cys Glu 
                245                 250                 255     


Phe Ser Leu Leu Lys Ser Glu Cys His Arg Val Lys Met Gln 
            260                 265                 270 


<210>  63
<211>  267
<212>  PRT
<213>  artificial sequence

<220>
<223>  no TM fusion 2 with inversion of neopeptides 1 and 2 and 7 and 8

<400>  63

Gly Leu Met Gly Ile Val Val Gly Thr Val Phe Ile Ile Arg Gly Leu 
1               5                   10                  15      


Arg Ser Val Gly Ala Ser Arg His Gln Gly Leu Gly Gln Ser Leu Pro 
            20                  25                  30          


Met Thr His Ser Leu Lys Leu Ser Lys Thr Asn Arg Thr Leu Phe Leu 
        35                  40                  45              


Leu Gly Val Thr Lys Tyr Trp Tyr Val Arg Met Asn Arg Arg Arg Leu 
    50                  55                  60                  


Lys Gly Glu Thr Gly Met Glu Asp Cys Val Met Ala Leu Glu Thr Leu 
65                  70                  75                  80  


Phe Thr Ala Ile Pro Leu Glu Ile Phe Ile Val Thr Met Glu Asn Asp 
                85                  90                  95      


Phe Pro Gly Gly Val Ile Gly Lys Ile His Ala Thr Gly Arg Gly Arg 
            100                 105                 110         


Ser Lys Leu Lys Ser Gly Ile Gly Ala Leu Val Leu Pro Gly Val Ser 
        115                 120                 125             


Thr Ala Asp Ile Ser Ser Asn His Gln Arg Glu Asn Ser Gly His Arg 
    130                 135                 140                 


Arg Asp Gln Ile Thr Glu Lys Asp Ala Ala Leu Cys Val Leu Ile Asp 
145                 150                 155                 160 


Glu Met Gly Trp Met Tyr Gly Thr Val Gln Arg Thr Gly Arg Thr Arg 
                165                 170                 175     


Met Leu Pro Ala Asn Tyr Val Glu Ala Ile His Asn Tyr Met Tyr Trp 
            180                 185                 190         


Tyr Arg Gln Asp Pro Gly Met Val Leu Lys Leu Ile Tyr Tyr Ser Val 
        195                 200                 205             


Gly Ala Gly Ile Thr Glu Glu Trp Glu Cys Leu Asp Ser Ala Gln Gln 
    210                 215                 220                 


Arg Leu His Arg Asp Val Met Leu Glu Asn Tyr Gly Asn Leu Phe Ser 
225                 230                 235                 240 


Leu Phe Thr Val Ser Pro Thr Leu Tyr Phe Leu His Ser Val Ser Leu 
                245                 250                 255     


Pro Ala Leu Asp Leu Lys Pro Gly Glu Gly Ala 
            260                 265         


<210>  64
<211>  189
<212>  PRT
<213>  artificial sequence

<220>
<223>  no TM fusion 3 by suppression of neopeptides 2, 4 and 5

<400>  64

Val Val Gly Trp Arg Ser Gly Val Glu Lys Asp Leu Asp Lys Val Leu 
1               5                   10                  15      


Gln Thr His Ser Val Phe Val Asn Val Ser Lys Glu Ala Thr Asn Ala 
            20                  25                  30          


Ser Val Phe Lys Tyr Tyr Ile His Gly Leu Ser Asp Leu Ile Asp Cys 
        35                  40                  45              


Cys Asp Leu Gly Tyr His Phe Leu Val Ile Ala Leu Lys Asn Ala Val 
    50                  55                  60                  


Glu Ile Tyr Val Trp Ala Pro Lys Pro Tyr His Lys Phe Met Ala Phe 
65                  70                  75                  80  


Lys Pro Gly Leu Asp Arg Glu Gln Leu Tyr Trp Glu Leu Ser Lys Leu 
                85                  90                  95      


Thr His Ser Ile Thr Glu Leu Gly Pro Tyr Thr Leu Leu Leu Gln Ile 
            100                 105                 110         


Ser Asn Trp Phe Ile Asn Ala Arg Arg Cys Ile Leu Pro Asp Met Leu 
        115                 120                 125             


Gln Gln Arg Arg Asn Asp Pro Thr Ile Ser Leu Ser Gly Cys Ala Val 
    130                 135                 140                 


Gln Met Phe Leu Ser Leu Ala Met Gly Thr Thr Glu Cys Val Leu Leu 
145                 150                 155                 160 


Gly Met Gln Ile Leu Ser Lys Leu Leu Leu Asp Leu Ser Cys Glu Phe 
                165                 170                 175     


Ser Leu Leu Lys Ser Glu Cys His Arg Val Lys Met Gln 
            180                 185                 


<210>  65
<211>  270
<212>  PRT
<213>  artificial sequence

<220>
<223>  fusion 3 encoded by pTG19290

<400>  65

Ser Ser Thr Ile Met Asp Val Asp Ser Thr Ile Ser Ser Trp Arg Ser 
1               5                   10                  15      


Thr Pro Ala Met Met Asn Gly Gln Gly Ser Thr Lys Ser Lys Lys Val 
            20                  25                  30          


Ser Asp Ile Lys Glu Glu Val Leu Ser Ala Ala Cys His Pro Val Gln 
        35                  40                  45              


His Ser Lys Val Glu Tyr Tyr His Ile Glu Val Met Lys Lys Lys Met 
    50                  55                  60                  


Tyr Ile Phe Trp Gly Lys Met Ser Arg Arg Thr Leu Leu Pro Ile Pro 
65                  70                  75                  80  


Thr Ser Trp Tyr Leu Glu Asn Ile Tyr Pro Asp Ser Gln Ile Thr Arg 
                85                  90                  95      


Arg Tyr Tyr Ser Leu Gly Glu Ile Arg Asn Val Glu Ser Pro Phe Asn 
            100                 105                 110         


Val Val Ala Trp His Gly Asn Tyr Thr Leu Tyr Lys Tyr Asn Leu Lys 
        115                 120                 125             


Asn Phe Met Val Ile Asn Ser Asp Tyr Glu Leu Ala Glu Val His Lys 
    130                 135                 140                 


Ala Leu Leu Asp Asn Lys Asn Lys Val Leu Glu Val Lys Lys Pro Pro 
145                 150                 155                 160 


Arg Phe Glu Lys Gly Pro Leu Pro Gly Glu Asp Trp Thr Ile Phe Lys 
                165                 170                 175     


Tyr Asn His Ser Thr Tyr Gln Pro Val Leu Leu Thr Glu Asp Arg Trp 
            180                 185                 190         


Val Arg Asn Gln Ala Asn Leu Asp Lys Glu His Val Pro Leu Leu Lys 
        195                 200                 205             


Ile Glu Glu Pro Pro Ser Thr Ala Leu Asp Thr Glu Glu Glu Glu Asp 
    210                 215                 220                 


Asp Thr Ile His Met Trp Asn Ala Ile Met Thr Phe Tyr Ser Ala Leu 
225                 230                 235                 240 


Ile Asp Leu Ser Thr Val Glu Lys His Cys Gly Lys Val Pro Tyr Pro 
                245                 250                 255     


Leu Ser Tyr Val Pro Ala Val Leu Ser Glu Leu Thr Asp Gln 
            260                 265                 270 


<210>  66
<211>  5
<212>  PRT
<213>  artificial sequence

<220>
<223>  linker 5aa

<400>  66

Gly Ser Gly Ser Gly 
1               5   


<210>  67
<211>  5
<212>  PRT
<213>  artificial sequence

<220>
<223>  linker of 5aa

<400>  67

Ser Gly Ser Gly Ser 
1               5   


<210>  68
<211>  10
<212>  PRT
<213>  artificial sequence

<220>
<223>  linker of 10aa

<400>  68

Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser 
1               5                   10  


<210>  69
<211>  5
<212>  PRT
<213>  artificial sequence

<220>
<223>  linker of 5aa

<400>  69

Gly Ser Thr Ser Gly 
1               5   


<210>  70
<211>  5
<212>  PRT
<213>  artificial sequence

<220>
<223>  linker of 5 aa

<400>  70

Ser Gly Thr Gly Ser 
1               5   


				RECTIFIED SHEET (RULE 91) ISA/EP
