                         SEQUENCE LISTING

<110>  OncoSec Medical Incorporated
       Canton, David A.
 
<120>  MULTIGENE CONSTRUCT FOR IMMUNE-MODULATORY PROTEIN EXPRESSION AND 
       METHODS OF USE

<130>  OM1702WO1  066914/516052

<150>  62/519,120
<151>  2017-06-13

<150>  62/582,917
<151>  2017-11-07

<150>  62/628,917
<151>  2018-02-09

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  6752
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  plasmid sequence with recombinant human gene sequences within

<400>  1
tggccattgc atacgttgta tccatatcat aatatgtaca tttatattgg ctcatgtcca       60

acattaccgc catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg      120

tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg      180

cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata      240

gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc      300

cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac      360

ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg      420

cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc      480

aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc      540

aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc      600

gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct      660

cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga      720

agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac gcggattccc      780

cgtgccaaga gtgacgtaag taccgcctat agactctata ggcacacccc tttggctctt      840

atgcatgcta tactgttttt ggcttggggc ctatacaccc ccgcttcctt atgctatagg      900

tgatggtata gcttagccta taggtgtggg ttattgacca ttattgacca ctccaacggt      960

ggagggcagt gtagtctgag cagtactcgt tgctgccgcg cgcgccacca gacataatag     1020

ctgacagact aacagactgt tcctttccat gggtcttttc tgcagtcacc gtcgtcgacg     1080

gtatcgataa gcttgatatc gaattcacgt gggcccggta ccaccatgtg gccccctggg     1140

tcagcctccc agccaccgcc ctcacctgcc gcggccacag gtctgcatcc agcggctcgc     1200

cctgtgtccc tgcagtgccg gctcagcatg tgtccagcgc gcagcctcct ccttgtggct     1260

accctggtcc tcctggacca cctcagtttg gccagaaacc tccccgtggc cactccagac     1320

ccaggaatgt tcccatgcct tcaccactcc caaaacctgc tgagggccgt cagcaacatg     1380

ctccagaagg ccagacaaac tctagaattt tacccttgca cttctgaaga gattgatcat     1440

gaagatatca caaaagataa aaccagcaca gtggaggcct gtttaccatt ggaattaacc     1500

aagaatgaga gttgcctaaa ttccagagag acctctttca taactaatgg gagttgcctg     1560

gcctccagaa agacctcttt tatgatggcc ctgtgcctta gtagtattta tgaagacttg     1620

aagatgtacc aggtggagtt caagaccatg aatgcaaagc ttctgatgga tcctaagagg     1680

cagatctttc tagatcaaaa catgctggca gttattgatg agctgatgca ggccctgaat     1740

ttcaacagtg agactgtgcc acaaaaatcc tcccttgaag aaccggattt ttataaaact     1800

aaaatcaagc tctgcatact tcttcatgct ttcagaattc gggcagtgac tattgataga     1860

gtgatgagct atctgaatgc ttccggatct ggggccacca acttttcatt gctcaagcag     1920

gcgggcgatg tggaggaaaa ccctggcccc tgtcaccagc agttggtcat ctcttggttt     1980

tccctggttt ttctggcatc tcccctcgtg gccatatggg aactgaagaa agatgtttat     2040

gtcgtagaat tggattggta tccggatgcc cctggagaaa tggtggtcct cacctgtgac     2100

acccctgaag aagatggtat cacctggacc ttggaccaga gcagtgaggt cttaggctct     2160

ggcaaaaccc tgaccatcca agtcaaagag tttggagatg ctggccagta cacctgtcac     2220

aaaggaggcg aggttctaag ccattcgctc ctgctgcttc acaaaaagga agatggaatt     2280

tggtccactg atattttaaa ggaccagaaa gaacccaaaa ataagacctt tctaagatgc     2340

gaggccaaga attattctgg acgtttcacc tgctggtggc tgacgacaat cagtactgat     2400

ttgacattca gtgtcaaaag cagcagaggc tcttctgacc cccaaggggt gacgtgcgga     2460

gctgctacac tctctgcaga gagagtcaga ggggacaaca aggagtatga gtactcagtg     2520

gagtgccagg aggacagtgc ctgcccagct gctgaggaga gtctgcccat tgaggtcatg     2580

gtggatgccg ttcacaagct caagtatgaa aactacacca gcagcttctt catcagggac     2640

atcatcaaac ctgacccacc caagaacttg cagctgaagc cattaaagaa ttctcggcag     2700

gtggaggtca gctgggagta ccctgacacc tggagtactc cacattccta cttctccctg     2760

acattctgcg ttcaggtcca gggcaagagc aagagagaaa agaaagatag agtcttcacg     2820

gacaagacct cagccacggt catctgccgc aaaaatgcca gcattagcgt gcgggcccag     2880

gaccgctact atagctcatc ttggagcgaa tgggcatctg tgccctgcag tggatctggg     2940

gccaccaact tttcattgct caagcaggcg ggcgatgtgg aggaaaaccc tggccccgag     3000

acagacacac tcctgctatg ggtactgctg ctctgggttc caggttccac tggtgacact     3060

caggattgca gcttccagca ttcacccata tcatcagatt ttgcagtaaa gatcagggaa     3120

ctctccgatt atctccttca agactacccc gtaacagtgg cctccaattt gcaagacgaa     3180

gagctttgtg gtgccctctg gcggctcgtt ttggcccaaa ggtggatgga acggcttaag     3240

acagtcgctg gcagcaagat gcaagggttg ctcgaacgag tcaatacaga gatccatttt     3300

gtaaccaagt gtgcatttca accgccgcca agctgccttc gctttgttca gacgaatata     3360

agtagactgt tgcaggaaac ctccgagcaa ctcgtagccc tgaagccctg gattacacgg     3420

caaaatttca gtcggtgcct tgagcttcag tgtcagcctg atagtagtac cttgcctccg     3480

ccatggtccc ccaggcctct tgaagctaca gctccgacag cccctcagcc gggcagtagt     3540

ggtagttctg gagccagggg gccggagagc cgcctgcttg agttctacct cgccatgcct     3600

ttcgcgacac ccatggaagc agagctggcc cgcaggagcc tggcccagga tgccccaccg     3660

cttcccgtgc caggggtgct tctgaaggag ttcactgtgt ccggcaacat actgactatc     3720

cgactgactg ctgcagacca ccgccaactg cagctctcca tcagctcctg tctccagcag     3780

ctttccctgt tgatgtggat cacgcagtgc tttctgcccg tgtttttggc tcagcctccc     3840

tcagggcaga ggcgctaagg ccgcggatcc agatcttttt ccctctgcca aaaattatgg     3900

ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat     3960

tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa     4020

tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg cccattcttc     4080

cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc     4140

tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat     4200

gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt     4260

ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg     4320

aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc     4380

tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt     4440

ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa     4500

gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta     4560

tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa     4620

caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa     4680

ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt     4740

cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt     4800

ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat     4860

cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat     4920

gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc     4980

aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc     5040

acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcg gggggggggg     5100

gcgctgaggt ctgcctcgtg aagaaggtgt tgctgactca taccaggcct gaatcgcccc     5160

atcatccagc cagaaagtga gggagccacg gttgatgaga gctttgttgt aggtggacca     5220

gttggtgatt ttgaactttt gctttgccac ggaacggtct gcgttgtcgg gaagatgcgt     5280

gatctgatcc ttcaactcag caaaagttcg atttattcaa caaagccgcc gtcccgtcaa     5340

gtcagcgtaa tgctctgcca gtgttacaac caattaacca attctgatta gaaaaactca     5400

tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga     5460

aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga     5520

tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc     5580

tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag     5640

aatggcaaaa gcttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg     5700

tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga     5760

cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc     5820

aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc     5880

tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg     5940

ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc     6000

tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca     6060

tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc     6120

catttatacc catataaatc agcatccatg ttggaattta atcgcggcct cgagcaagac     6180

gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt     6240

tttattgttc atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac     6300

acaacgtggc tttccccccc cccccattat tgaagcattt atcagggtta ttgtctcatg     6360

agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt     6420

ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa     6480

aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc     6540

tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga     6600

caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg     6660

gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc     6720

gtaaggagaa aataccgcat cagattggct at                                   6752


<210>  2
<211>  274
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human recombinant protein

<400>  2

Met Trp Pro Pro Gly Ser Ala Ser Gln Pro Pro Pro Ser Pro Ala Ala 
1               5                   10                  15      


Ala Thr Gly Leu His Pro Ala Ala Arg Pro Val Ser Leu Gln Cys Arg 
            20                  25                  30          


Leu Ser Met Cys Pro Ala Arg Ser Leu Leu Leu Val Ala Thr Leu Val 
        35                  40                  45              


Leu Leu Asp His Leu Ser Leu Ala Arg Asn Leu Pro Val Ala Thr Pro 
    50                  55                  60                  


Asp Pro Gly Met Phe Pro Cys Leu His His Ser Gln Asn Leu Leu Arg 
65                  70                  75                  80  


Ala Val Ser Asn Met Leu Gln Lys Ala Arg Gln Thr Leu Glu Phe Tyr 
                85                  90                  95      


Pro Cys Thr Ser Glu Glu Ile Asp His Glu Asp Ile Thr Lys Asp Lys 
            100                 105                 110         


Thr Ser Thr Val Glu Ala Cys Leu Pro Leu Glu Leu Thr Lys Asn Glu 
        115                 120                 125             


Ser Cys Leu Asn Ser Arg Glu Thr Ser Phe Ile Thr Asn Gly Ser Cys 
    130                 135                 140                 


Leu Ala Ser Arg Lys Thr Ser Phe Met Met Ala Leu Cys Leu Ser Ser 
145                 150                 155                 160 


Ile Tyr Glu Asp Leu Lys Met Tyr Gln Val Glu Phe Lys Thr Met Asn 
                165                 170                 175     


Ala Lys Leu Leu Met Asp Pro Lys Arg Gln Ile Phe Leu Asp Gln Asn 
            180                 185                 190         


Met Leu Ala Val Ile Asp Glu Leu Met Gln Ala Leu Asn Phe Asn Ser 
        195                 200                 205             


Glu Thr Val Pro Gln Lys Ser Ser Leu Glu Glu Pro Asp Phe Tyr Lys 
    210                 215                 220                 


Thr Lys Ile Lys Leu Cys Ile Leu Leu His Ala Phe Arg Ile Arg Ala 
225                 230                 235                 240 


Val Thr Ile Asp Arg Val Met Ser Tyr Leu Asn Ala Ser Gly Ser Gly 
                245                 250                 255     


Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
            260                 265                 270         


Pro Gly 
        


<210>  3
<211>  349
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant human protein

<400>  3

Pro Cys His Gln Gln Leu Val Ile Ser Trp Phe Ser Leu Val Phe Leu 
1               5                   10                  15      


Ala Ser Pro Leu Val Ala Ile Trp Glu Leu Lys Lys Asp Val Tyr Val 
            20                  25                  30          


Val Glu Leu Asp Trp Tyr Pro Asp Ala Pro Gly Glu Met Val Val Leu 
        35                  40                  45              


Thr Cys Asp Thr Pro Glu Glu Asp Gly Ile Thr Trp Thr Leu Asp Gln 
    50                  55                  60                  


Ser Ser Glu Val Leu Gly Ser Gly Lys Thr Leu Thr Ile Gln Val Lys 
65                  70                  75                  80  


Glu Phe Gly Asp Ala Gly Gln Tyr Thr Cys His Lys Gly Gly Glu Val 
                85                  90                  95      


Leu Ser His Ser Leu Leu Leu Leu His Lys Lys Glu Asp Gly Ile Trp 
            100                 105                 110         


Ser Thr Asp Ile Leu Lys Asp Gln Lys Glu Pro Lys Asn Lys Thr Phe 
        115                 120                 125             


Leu Arg Cys Glu Ala Lys Asn Tyr Ser Gly Arg Phe Thr Cys Trp Trp 
    130                 135                 140                 


Leu Thr Thr Ile Ser Thr Asp Leu Thr Phe Ser Val Lys Ser Ser Arg 
145                 150                 155                 160 


Gly Ser Ser Asp Pro Gln Gly Val Thr Cys Gly Ala Ala Thr Leu Ser 
                165                 170                 175     


Ala Glu Arg Val Arg Gly Asp Asn Lys Glu Tyr Glu Tyr Ser Val Glu 
            180                 185                 190         


Cys Gln Glu Asp Ser Ala Cys Pro Ala Ala Glu Glu Ser Leu Pro Ile 
        195                 200                 205             


Glu Val Met Val Asp Ala Val His Lys Leu Lys Tyr Glu Asn Tyr Thr 
    210                 215                 220                 


Ser Ser Phe Phe Ile Arg Asp Ile Ile Lys Pro Asp Pro Pro Lys Asn 
225                 230                 235                 240 


Leu Gln Leu Lys Pro Leu Lys Asn Ser Arg Gln Val Glu Val Ser Trp 
                245                 250                 255     


Glu Tyr Pro Asp Thr Trp Ser Thr Pro His Ser Tyr Phe Ser Leu Thr 
            260                 265                 270         


Phe Cys Val Gln Val Gln Gly Lys Ser Lys Arg Glu Lys Lys Asp Arg 
        275                 280                 285             


Val Phe Thr Asp Lys Thr Ser Ala Thr Val Ile Cys Arg Lys Asn Ala 
    290                 295                 300                 


Ser Ile Ser Val Arg Ala Gln Asp Arg Tyr Tyr Ser Ser Ser Trp Ser 
305                 310                 315                 320 


Glu Trp Ala Ser Val Pro Cys Ser Gly Ser Gly Ala Thr Asn Phe Ser 
                325                 330                 335     


Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly 
            340                 345                 


<210>  4
<211>  287
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant human protein

<400>  4

Pro Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Thr Gln Asp Cys Ser Phe Gln His Ser Pro Ile 
            20                  25                  30          


Ser Ser Asp Phe Ala Val Lys Ile Arg Glu Leu Ser Asp Tyr Leu Leu 
        35                  40                  45              


Gln Asp Tyr Pro Val Thr Val Ala Ser Asn Leu Gln Asp Glu Glu Leu 
    50                  55                  60                  


Cys Gly Ala Leu Trp Arg Leu Val Leu Ala Gln Arg Trp Met Glu Arg 
65                  70                  75                  80  


Leu Lys Thr Val Ala Gly Ser Lys Met Gln Gly Leu Leu Glu Arg Val 
                85                  90                  95      


Asn Thr Glu Ile His Phe Val Thr Lys Cys Ala Phe Gln Pro Pro Pro 
            100                 105                 110         


Ser Cys Leu Arg Phe Val Gln Thr Asn Ile Ser Arg Leu Leu Gln Glu 
        115                 120                 125             


Thr Ser Glu Gln Leu Val Ala Leu Lys Pro Trp Ile Thr Arg Gln Asn 
    130                 135                 140                 


Phe Ser Arg Cys Leu Glu Leu Gln Cys Gln Pro Asp Ser Ser Thr Leu 
145                 150                 155                 160 


Pro Pro Pro Trp Ser Pro Arg Pro Leu Glu Ala Thr Ala Pro Thr Ala 
                165                 170                 175     


Pro Gln Pro Gly Ser Ser Gly Ser Ser Gly Ala Arg Gly Pro Glu Ser 
            180                 185                 190         


Arg Leu Leu Glu Phe Tyr Leu Ala Met Pro Phe Ala Thr Pro Met Glu 
        195                 200                 205             


Ala Glu Leu Ala Arg Arg Ser Leu Ala Gln Asp Ala Pro Pro Leu Pro 
    210                 215                 220                 


Val Pro Gly Val Leu Leu Lys Glu Phe Thr Val Ser Gly Asn Ile Leu 
225                 230                 235                 240 


Thr Ile Arg Leu Thr Ala Ala Asp His Arg Gln Leu Gln Leu Ser Ile 
                245                 250                 255     


Ser Ser Cys Leu Gln Gln Leu Ser Leu Leu Met Trp Ile Thr Gln Cys 
            260                 265                 270         


Phe Leu Pro Val Phe Leu Ala Gln Pro Pro Ser Gly Gln Arg Arg 
        275                 280                 285         


