                         SEQUENCE LISTING

<110>  Autolus Limited
 
<120>  CHIMERIC ANTIGEN RECEPTOR

<130>  P112492PCT

<150>  GB 1709508.4
<151>  2017-06-15

<160>  40    

<170>  PatentIn version 3.5

<210>  1
<211>  250
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Pro Ala Ser Ser Pro Phe Leu Leu Ala Pro Lys Gly Pro Pro Gly 
1               5                   10                  15      


Asn Met Gly Gly Pro Val Arg Glu Pro Ala Leu Ser Val Ala Leu Trp 
            20                  25                  30          


Leu Ser Trp Gly Ala Ala Leu Gly Ala Val Ala Cys Ala Met Ala Leu 
        35                  40                  45              


Leu Thr Gln Gln Thr Glu Leu Gln Ser Leu Arg Arg Glu Val Ser Arg 
    50                  55                  60                  


Leu Gln Gly Thr Gly Gly Pro Ser Gln Asn Gly Glu Gly Tyr Pro Trp 
65                  70                  75                  80  


Gln Ser Leu Pro Glu Gln Ser Ser Asp Ala Leu Glu Ala Trp Glu Asn 
                85                  90                  95      


Gly Glu Arg Ser Arg Lys Arg Arg Ala Val Leu Thr Gln Lys Gln Lys 
            100                 105                 110         


Lys Gln His Ser Val Leu His Leu Val Pro Ile Asn Ala Thr Ser Lys 
        115                 120                 125             


Asp Asp Ser Asp Val Thr Glu Val Met Trp Gln Pro Ala Leu Arg Arg 
    130                 135                 140                 


Gly Arg Gly Leu Gln Ala Gln Gly Tyr Gly Val Arg Ile Gln Asp Ala 
145                 150                 155                 160 


Gly Val Tyr Leu Leu Tyr Ser Gln Val Leu Phe Gln Asp Val Thr Phe 
                165                 170                 175     


Thr Met Gly Gln Val Val Ser Arg Glu Gly Gln Gly Arg Gln Glu Thr 
            180                 185                 190         


Leu Phe Arg Cys Ile Arg Ser Met Pro Ser His Pro Asp Arg Ala Tyr 
        195                 200                 205             


Asn Ser Cys Tyr Ser Ala Gly Val Phe His Leu His Gln Gly Asp Ile 
    210                 215                 220                 


Leu Ser Val Ile Ile Pro Arg Ala Arg Ala Lys Leu Asn Leu Ser Pro 
225                 230                 235                 240 


His Gly Thr Phe Leu Gly Phe Val Lys Leu 
                245                 250 


<210>  2
<211>  134
<212>  PRT
<213>  Homo sapiens

<400>  2

Val Leu His Leu Val Pro Ile Asn Ala Thr Ser Lys Asp Asp Ser Asp 
1               5                   10                  15      


Val Thr Glu Val Met Trp Gln Pro Ala Leu Arg Arg Gly Arg Gly Leu 
            20                  25                  30          


Gln Ala Gln Gly Tyr Gly Val Arg Ile Gln Asp Ala Gly Val Tyr Leu 
        35                  40                  45              


Leu Tyr Ser Gln Val Leu Phe Gln Asp Val Thr Phe Thr Met Gly Gln 
    50                  55                  60                  


Val Val Ser Arg Glu Gly Gln Gly Arg Gln Glu Thr Leu Phe Arg Cys 
65                  70                  75                  80  


Ile Arg Ser Met Pro Ser His Pro Asp Arg Ala Tyr Asn Ser Cys Tyr 
                85                  90                  95      


Ser Ala Gly Val Phe His Leu His Gln Gly Asp Ile Leu Ser Val Ile 
            100                 105                 110         


Ile Pro Arg Ala Arg Ala Lys Leu Asn Leu Ser Pro His Gly Thr Phe 
        115                 120                 125             


Leu Gly Phe Val Lys Leu 
    130                 


<210>  3
<211>  824
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric antigen receptor (CAR) configuration, 
       TIP-CD28-OX40-z-2A-APRIL-HNG-TM-L-TetRB

<400>  3

Met Trp Thr Trp Asn Ala Tyr Ala Phe Ala Ala Pro Ser Gly Gly Gly 
1               5                   10                  15      


Ser Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met 
            20                  25                  30          


Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala 
        35                  40                  45              


Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Asp Gln Arg Leu Pro 
    50                  55                  60                  


Pro Asp Ala His Lys Pro Pro Gly Gly Gly Ser Phe Arg Thr Pro Ile 
65                  70                  75                  80  


Gln Glu Glu Gln Ala Asp Ala His Ser Thr Leu Ala Lys Ile Arg Val 
                85                  90                  95      


Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn 
            100                 105                 110         


Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val 
        115                 120                 125             


Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg 
    130                 135                 140                 


Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys 
145                 150                 155                 160 


Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg 
                165                 170                 175     


Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys 
            180                 185                 190         


Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Ala Glu 
        195                 200                 205             


Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly 
    210                 215                 220                 


Pro Met Gly Thr Ser Leu Leu Cys Trp Met Ala Leu Cys Leu Leu Gly 
225                 230                 235                 240 


Ala Asp His Ala Asp Ala Cys Pro Tyr Ser Asn Pro Ser Leu Cys Ser 
                245                 250                 255     


Gly Gly Gly Gly Ser Glu Leu Pro Thr Gln Gly Thr Phe Ser Asn Val 
            260                 265                 270         


Ser Thr Asn Val Ser Pro Ala Lys Pro Thr Thr Thr Ala Cys Pro Tyr 
        275                 280                 285             


Ser Asn Pro Ser Leu Cys Ser Gly Gly Gly Gly Ser Pro Ala Pro Arg 
    290                 295                 300                 


Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg 
305                 310                 315                 320 


Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly 
                325                 330                 335     


Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr 
            340                 345                 350         


Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn His 
        355                 360                 365             


Arg Asn Arg Arg Arg Val Cys Lys Cys Pro Arg Pro Val Val Arg Ala 
    370                 375                 380                 


Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
385                 390                 395                 400 


Gly Pro Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp 
                405                 410                 415     


Val Pro Gly Ser Thr Gly Ser Val Leu His Leu Val Pro Ile Asn Ala 
            420                 425                 430         


Thr Ser Lys Asp Asp Ser Asp Val Thr Glu Val Met Trp Gln Pro Ala 
        435                 440                 445             


Leu Arg Arg Gly Arg Gly Leu Gln Ala Gln Gly Tyr Gly Val Arg Ile 
    450                 455                 460                 


Gln Asp Ala Gly Val Tyr Leu Leu Tyr Ser Gln Val Leu Phe Gln Asp 
465                 470                 475                 480 


Val Thr Phe Thr Met Gly Gln Val Val Ser Arg Glu Gly Gln Gly Arg 
                485                 490                 495     


Gln Glu Thr Leu Phe Arg Cys Ile Arg Ser Met Pro Ser His Pro Asp 
            500                 505                 510         


Arg Ala Tyr Asn Ser Cys Tyr Ser Ala Gly Val Phe His Leu His Gln 
        515                 520                 525             


Gly Asp Ile Leu Ser Val Ile Ile Pro Arg Ala Arg Ala Lys Leu Asn 
    530                 535                 540                 


Leu Ser Pro His Gly Thr Phe Leu Gly Phe Val Lys Leu Ser Gly Gly 
545                 550                 555                 560 


Gly Ser Asp Pro Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys 
                565                 570                 575     


Pro Pro Cys Pro Lys Asp Pro Lys Phe Trp Val Leu Val Val Val Gly 
            580                 585                 590         


Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile 
        595                 600                 605             


Phe Trp Val Ser Gly Gly Gly Gly Ser Met Ser Arg Leu Asp Lys Ser 
    610                 615                 620                 


Lys Val Ile Asn Ser Ala Leu Glu Leu Leu Asn Glu Val Gly Ile Glu 
625                 630                 635                 640 


Gly Leu Thr Thr Arg Lys Leu Ala Gln Lys Leu Gly Val Glu Gln Pro 
                645                 650                 655     


Thr Leu Tyr Trp His Val Lys Asn Lys Arg Ala Leu Leu Asp Ala Leu 
            660                 665                 670         


Ala Ile Glu Met Leu Asp Arg His His Thr His Phe Cys Pro Leu Glu 
        675                 680                 685             


Gly Glu Ser Trp Gln Asp Phe Leu Arg Asn Asn Ala Lys Ser Phe Arg 
    690                 695                 700                 


Cys Ala Leu Leu Ser His Arg Asp Gly Ala Lys Val His Leu Gly Thr 
705                 710                 715                 720 


Arg Pro Thr Glu Lys Gln Tyr Glu Thr Leu Glu Asn Gln Leu Ala Phe 
                725                 730                 735     


Leu Cys Gln Gln Gly Phe Ser Leu Glu Asn Ala Leu Tyr Ala Leu Ser 
            740                 745                 750         


Ala Val Gly His Phe Thr Leu Gly Cys Val Leu Glu Asp Gln Glu His 
        755                 760                 765             


Gln Val Ala Lys Glu Glu Arg Glu Thr Pro Thr Thr Asp Ser Met Pro 
    770                 775                 780                 


Pro Leu Leu Arg Gln Ala Ile Glu Leu Phe Asp His Gln Gly Ala Glu 
785                 790                 795                 800 


Pro Ala Phe Leu Phe Gly Leu Glu Leu Ile Ile Cys Gly Leu Glu Lys 
                805                 810                 815     


Gln Leu Lys Cys Glu Ser Gly Ser 
            820                 


<210>  4
<211>  824
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR configuration, TIP-OX40-z-2A-APRIL-HNG-CD28TM-CD28-L-TetRB

<400>  4

Met Trp Thr Trp Asn Ala Tyr Ala Phe Ala Ala Pro Ser Gly Gly Gly 
1               5                   10                  15      


Ser Arg Asp Gln Arg Leu Pro Pro Asp Ala His Lys Pro Pro Gly Gly 
            20                  25                  30          


Gly Ser Phe Arg Thr Pro Ile Gln Glu Glu Gln Ala Asp Ala His Ser 
        35                  40                  45              


Thr Leu Ala Lys Ile Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro 
    50                  55                  60                  


Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly 
65                  70                  75                  80  


Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro 
                85                  90                  95      


Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr 
            100                 105                 110         


Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly 
        115                 120                 125             


Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln 
    130                 135                 140                 


Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln 
145                 150                 155                 160 


Ala Leu Pro Pro Arg Ala Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly 
                165                 170                 175     


Asp Val Glu Glu Asn Pro Gly Pro Met Gly Thr Ser Leu Leu Cys Trp 
            180                 185                 190         


Met Ala Leu Cys Leu Leu Gly Ala Asp His Ala Asp Ala Cys Pro Tyr 
        195                 200                 205             


Ser Asn Pro Ser Leu Cys Ser Gly Gly Gly Gly Ser Glu Leu Pro Thr 
    210                 215                 220                 


Gln Gly Thr Phe Ser Asn Val Ser Thr Asn Val Ser Pro Ala Lys Pro 
225                 230                 235                 240 


Thr Thr Thr Ala Cys Pro Tyr Ser Asn Pro Ser Leu Cys Ser Gly Gly 
                245                 250                 255     


Gly Gly Ser Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
            260                 265                 270         


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
        275                 280                 285             


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile 
    290                 295                 300                 


Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val 
305                 310                 315                 320 


Ile Thr Leu Tyr Cys Asn His Arg Asn Arg Arg Arg Val Cys Lys Cys 
                325                 330                 335     


Pro Arg Pro Val Val Arg Ala Glu Gly Arg Gly Ser Leu Leu Thr Cys 
            340                 345                 350         


Gly Asp Val Glu Glu Asn Pro Gly Pro Met Glu Thr Asp Thr Leu Leu 
        355                 360                 365             


Leu Trp Val Leu Leu Leu Trp Val Pro Gly Ser Thr Gly Ser Val Leu 
    370                 375                 380                 


His Leu Val Pro Ile Asn Ala Thr Ser Lys Asp Asp Ser Asp Val Thr 
385                 390                 395                 400 


Glu Val Met Trp Gln Pro Ala Leu Arg Arg Gly Arg Gly Leu Gln Ala 
                405                 410                 415     


Gln Gly Tyr Gly Val Arg Ile Gln Asp Ala Gly Val Tyr Leu Leu Tyr 
            420                 425                 430         


Ser Gln Val Leu Phe Gln Asp Val Thr Phe Thr Met Gly Gln Val Val 
        435                 440                 445             


Ser Arg Glu Gly Gln Gly Arg Gln Glu Thr Leu Phe Arg Cys Ile Arg 
    450                 455                 460                 


Ser Met Pro Ser His Pro Asp Arg Ala Tyr Asn Ser Cys Tyr Ser Ala 
465                 470                 475                 480 


Gly Val Phe His Leu His Gln Gly Asp Ile Leu Ser Val Ile Ile Pro 
                485                 490                 495     


Arg Ala Arg Ala Lys Leu Asn Leu Ser Pro His Gly Thr Phe Leu Gly 
            500                 505                 510         


Phe Val Lys Leu Ser Gly Gly Gly Ser Asp Pro Ala Glu Pro Lys Ser 
        515                 520                 525             


Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro Lys Asp Pro Lys Phe 
    530                 535                 540                 


Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu 
545                 550                 555                 560 


Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg 
                565                 570                 575     


Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro 
            580                 585                 590         


Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala 
        595                 600                 605             


Tyr Arg Ser Ser Gly Gly Gly Gly Ser Met Ser Arg Leu Asp Lys Ser 
    610                 615                 620                 


Lys Val Ile Asn Ser Ala Leu Glu Leu Leu Asn Glu Val Gly Ile Glu 
625                 630                 635                 640 


Gly Leu Thr Thr Arg Lys Leu Ala Gln Lys Leu Gly Val Glu Gln Pro 
                645                 650                 655     


Thr Leu Tyr Trp His Val Lys Asn Lys Arg Ala Leu Leu Asp Ala Leu 
            660                 665                 670         


Ala Ile Glu Met Leu Asp Arg His His Thr His Phe Cys Pro Leu Glu 
        675                 680                 685             


Gly Glu Ser Trp Gln Asp Phe Leu Arg Asn Asn Ala Lys Ser Phe Arg 
    690                 695                 700                 


Cys Ala Leu Leu Ser His Arg Asp Gly Ala Lys Val His Leu Gly Thr 
705                 710                 715                 720 


Arg Pro Thr Glu Lys Gln Tyr Glu Thr Leu Glu Asn Gln Leu Ala Phe 
                725                 730                 735     


Leu Cys Gln Gln Gly Phe Ser Leu Glu Asn Ala Leu Tyr Ala Leu Ser 
            740                 745                 750         


Ala Val Gly His Phe Thr Leu Gly Cys Val Leu Glu Asp Gln Glu His 
        755                 760                 765             


Gln Val Ala Lys Glu Glu Arg Glu Thr Pro Thr Thr Asp Ser Met Pro 
    770                 775                 780                 


Pro Leu Leu Arg Gln Ala Ile Glu Leu Phe Asp His Gln Gly Ala Glu 
785                 790                 795                 800 


Pro Ala Phe Leu Phe Gly Leu Glu Leu Ile Ile Cys Gly Leu Glu Lys 
                805                 810                 815     


Gln Leu Lys Cys Glu Ser Gly Ser 
            820                 


<210>  5
<211>  824
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR configuration, TIP-z-2A-APRIL-HNG-CD28TM-CD28-OX40-L-TetRB

<400>  5

Met Trp Thr Trp Asn Ala Tyr Ala Phe Ala Ala Pro Ser Gly Gly Gly 
1               5                   10                  15      


Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln 
            20                  25                  30          


Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
        35                  40                  45              


Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly 
    50                  55                  60                  


Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
65                  70                  75                  80  


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
                85                  90                  95      


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
            100                 105                 110         


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
        115                 120                 125             


Arg Ala Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu 
    130                 135                 140                 


Asn Pro Gly Pro Met Gly Thr Ser Leu Leu Cys Trp Met Ala Leu Cys 
145                 150                 155                 160 


Leu Leu Gly Ala Asp His Ala Asp Ala Cys Pro Tyr Ser Asn Pro Ser 
                165                 170                 175     


Leu Cys Ser Gly Gly Gly Gly Ser Glu Leu Pro Thr Gln Gly Thr Phe 
            180                 185                 190         


Ser Asn Val Ser Thr Asn Val Ser Pro Ala Lys Pro Thr Thr Thr Ala 
        195                 200                 205             


Cys Pro Tyr Ser Asn Pro Ser Leu Cys Ser Gly Gly Gly Gly Ser Pro 
    210                 215                 220                 


Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu 
225                 230                 235                 240 


Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His 
                245                 250                 255     


Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu 
            260                 265                 270         


Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr 
        275                 280                 285             


Cys Asn His Arg Asn Arg Arg Arg Val Cys Lys Cys Pro Arg Pro Val 
    290                 295                 300                 


Val Arg Ala Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu 
305                 310                 315                 320 


Glu Asn Pro Gly Pro Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu 
                325                 330                 335     


Leu Leu Trp Val Pro Gly Ser Thr Gly Ser Val Leu His Leu Val Pro 
            340                 345                 350         


Ile Asn Ala Thr Ser Lys Asp Asp Ser Asp Val Thr Glu Val Met Trp 
        355                 360                 365             


Gln Pro Ala Leu Arg Arg Gly Arg Gly Leu Gln Ala Gln Gly Tyr Gly 
    370                 375                 380                 


Val Arg Ile Gln Asp Ala Gly Val Tyr Leu Leu Tyr Ser Gln Val Leu 
385                 390                 395                 400 


Phe Gln Asp Val Thr Phe Thr Met Gly Gln Val Val Ser Arg Glu Gly 
                405                 410                 415     


Gln Gly Arg Gln Glu Thr Leu Phe Arg Cys Ile Arg Ser Met Pro Ser 
            420                 425                 430         


His Pro Asp Arg Ala Tyr Asn Ser Cys Tyr Ser Ala Gly Val Phe His 
        435                 440                 445             


Leu His Gln Gly Asp Ile Leu Ser Val Ile Ile Pro Arg Ala Arg Ala 
    450                 455                 460                 


Lys Leu Asn Leu Ser Pro His Gly Thr Phe Leu Gly Phe Val Lys Leu 
465                 470                 475                 480 


Ser Gly Gly Gly Ser Asp Pro Ala Glu Pro Lys Ser Pro Asp Lys Thr 
                485                 490                 495     


His Thr Cys Pro Pro Cys Pro Lys Asp Pro Lys Phe Trp Val Leu Val 
            500                 505                 510         


Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala 
        515                 520                 525             


Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser 
    530                 535                 540                 


Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His 
545                 550                 555                 560 


Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg 
                565                 570                 575     


Asp Gln Arg Leu Pro Pro Asp Ala His Lys Pro Pro Gly Gly Gly Ser 
            580                 585                 590         


Phe Arg Thr Pro Ile Gln Glu Glu Gln Ala Asp Ala His Ser Thr Leu 
        595                 600                 605             


Ala Lys Ile Ser Gly Gly Gly Gly Ser Met Ser Arg Leu Asp Lys Ser 
    610                 615                 620                 


Lys Val Ile Asn Ser Ala Leu Glu Leu Leu Asn Glu Val Gly Ile Glu 
625                 630                 635                 640 


Gly Leu Thr Thr Arg Lys Leu Ala Gln Lys Leu Gly Val Glu Gln Pro 
                645                 650                 655     


Thr Leu Tyr Trp His Val Lys Asn Lys Arg Ala Leu Leu Asp Ala Leu 
            660                 665                 670         


Ala Ile Glu Met Leu Asp Arg His His Thr His Phe Cys Pro Leu Glu 
        675                 680                 685             


Gly Glu Ser Trp Gln Asp Phe Leu Arg Asn Asn Ala Lys Ser Phe Arg 
    690                 695                 700                 


Cys Ala Leu Leu Ser His Arg Asp Gly Ala Lys Val His Leu Gly Thr 
705                 710                 715                 720 


Arg Pro Thr Glu Lys Gln Tyr Glu Thr Leu Glu Asn Gln Leu Ala Phe 
                725                 730                 735     


Leu Cys Gln Gln Gly Phe Ser Leu Glu Asn Ala Leu Tyr Ala Leu Ser 
            740                 745                 750         


Ala Val Gly His Phe Thr Leu Gly Cys Val Leu Glu Asp Gln Glu His 
        755                 760                 765             


Gln Val Ala Lys Glu Glu Arg Glu Thr Pro Thr Thr Asp Ser Met Pro 
    770                 775                 780                 


Pro Leu Leu Arg Gln Ala Ile Glu Leu Phe Asp His Gln Gly Ala Glu 
785                 790                 795                 800 


Pro Ala Phe Leu Phe Gly Leu Glu Leu Ile Ile Cys Gly Leu Glu Lys 
                805                 810                 815     


Gln Leu Lys Cys Glu Ser Gly Ser 
            820                 


<210>  6
<211>  139
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heterodimerization domain

<400>  6

Met Ser Arg Leu Asp Lys Ser Lys Val Ile Asn Ser Ala Leu Glu Leu 
1               5                   10                  15      


Leu Asn Glu Val Gly Ile Glu Gly Leu Thr Thr Arg Lys Leu Ala Gln 
            20                  25                  30          


Lys Leu Gly Val Glu Gln Pro Thr Leu Tyr Trp His Val Lys Asn Lys 
        35                  40                  45              


Arg Ala Leu Leu Asp Ala Leu Ala Ile Glu Met Leu Asp Arg His His 
    50                  55                  60                  


Thr His Phe Cys Pro Leu Glu Gly Glu Ser Trp Gln Asp Phe Leu Arg 
65                  70                  75                  80  


Asn Asn Ala Lys Ser Phe Arg Cys Ala Leu Leu Ser His Arg Asp Gly 
                85                  90                  95      


Ala Lys Val His Leu Gly Thr Arg Pro Thr Glu Lys Gln Tyr Glu Thr 
            100                 105                 110         


Leu Glu Asn Gln Leu Ala Phe Leu Cys Gln Gln Gly Phe Ser Leu Glu 
        115                 120                 125             


Asn Ala Leu Tyr Ala Leu Ser Ala Val Gly His 
    130                 135                 


<210>  7
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heterodimerization domain

<400>  7

Met Trp Thr Trp Asn Ala Tyr Ala Phe Ala Ala Pro 
1               5                   10          


<210>  8
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  8

Ser Gly Gly Gly Ser 
1               5   


<210>  9
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  9

Ser Gly Gly Gly Gly Ser 
1               5       


<210>  10
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  10

Pro Cys Pro Lys Asp Pro Lys 
1               5           


<210>  11
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  11

Ser Gly Gly Gly Ser Asp Pro Ala 
1               5               


<210>  12
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Biotin mimicking peptide, long nanotag

<400>  12

Asp Val Glu Ala Trp Leu Asp Glu Arg Val Pro Leu Val Glu Thr 
1               5                   10                  15  


<210>  13
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Biotin mimicking peptide, short nanotag

<400>  13

Asp Val Glu Ala Trp Leu Gly Ala Arg 
1               5                   


<210>  14
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Biotin mimicking peptide, streptag

<400>  14

Trp Arg His Pro Gln Phe Gly Gly 
1               5               


<210>  15
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Biotin mimicking peptide, streptagII

<400>  15

Trp Ser His Pro Gln Phe Glu Lys 
1               5               


<210>  16
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Biotin mimicking peptide, SBP-tag

<400>  16

Met Asp Glu Lys Thr Thr Gly Trp Arg Gly Gly His Val Val Glu Gly 
1               5                   10                  15      


Leu Ala Gly Glu Leu Glu Gln Leu Arg Ala Arg Leu Glu His His Pro 
            20                  25                  30          


Gln Gly Gln Arg Glu Pro 
        35              


<210>  17
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Biotin mimicking peptide, ccstreptag

<400>  17

Cys His Pro Gln Gly Pro Pro Cys 
1               5               


<210>  18
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Biotin mimicking peptide, flankedccstreptag

<400>  18

Ala Glu Cys His Pro Gln Gly Pro Pro Cys Ile Glu Gly Arg Lys 
1               5                   10                  15  


<210>  19
<211>  126
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  core streptavidin sequence

<400>  19

Glu Ala Gly Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr Phe 
1               5                   10                  15      


Ile Val Thr Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Glu Ser 
            20                  25                  30          


Ala Val Gly Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr Asp 
        35                  40                  45              


Ser Ala Pro Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr Val 
    50                  55                  60                  


Ala Trp Lys Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp Ser 
65                  70                  75                  80  


Gly Gln Tyr Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp Leu 
                85                  90                  95      


Leu Thr Ser Gly Thr Thr Glu Ala Asn Ala Trp Lys Ser Thr Leu Val 
            100                 105                 110         


Gly His Asp Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser 
        115                 120                 125     


<210>  20
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  tyrp-1 transmembrane sequence

<400>  20

Ile Ile Ala Ile Ala Val Val Gly Ala Leu Leu Leu Val Ala Leu Ile 
1               5                   10                  15      


Phe Gly Thr Ala Ser Tyr Leu Ile 
            20                  


<210>  21
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3-zeta endodomain

<400>  21

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  22
<211>  140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 transmembrane domain and CD3-zeta endodomain

<400>  22

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Arg Val Lys Phe 
            20                  25                  30          


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
        35                  40                  45              


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
    50                  55                  60                  


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
65                  70                  75                  80  


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
                85                  90                  95      


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
            100                 105                 110         


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
        115                 120                 125             


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
    130                 135                 140 


<210>  23
<211>  180
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 transmembrane domain, CD28 endodomain and CD3-zeta 
       endodomains

<400>  23

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser 
            20                  25                  30          


Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly 
        35                  40                  45              


Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala 
    50                  55                  60                  


Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala 
65                  70                  75                  80  


Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg 
                85                  90                  95      


Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu 
            100                 105                 110         


Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
        115                 120                 125             


Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
    130                 135                 140                 


Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
145                 150                 155                 160 


Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala 
                165                 170                 175     


Leu Pro Pro Arg 
            180 


<210>  24
<211>  216
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 transmembrane domain and CD28 endodomain, OX40 endodomain 
       and CD3-zeta endodomain

<400>  24

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser 
            20                  25                  30          


Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly 
        35                  40                  45              


Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala 
    50                  55                  60                  


Ala Tyr Arg Ser Arg Asp Gln Arg Leu Pro Pro Asp Ala His Lys Pro 
65                  70                  75                  80  


Pro Gly Gly Gly Ser Phe Arg Thr Pro Ile Gln Glu Glu Gln Ala Asp 
                85                  90                  95      


Ala His Ser Thr Leu Ala Lys Ile Arg Val Lys Phe Ser Arg Ser Ala 
            100                 105                 110         


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
        115                 120                 125             


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
    130                 135                 140                 


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
145                 150                 155                 160 


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
                165                 170                 175     


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
            180                 185                 190         


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
        195                 200                 205             


His Met Gln Ala Leu Pro Pro Arg 
    210                 215     


<210>  25
<211>  20
<212>  PRT
<213>  Thosea asigna

<400>  25

Arg Ala Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  26
<211>  2471
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  APRIL CAR system, TIP-OX40-z-2A-SP-APRIL-HNG-TM-CD28-L-TetRB

<400>  26
atgtggacct ggaacgccta cgcctttgcc gcccctagcg gaggcggatc tagggaccag       60

aggctgcccc ccgatgccca caagccccct gggggaggca gtttccggac ccccatccaa      120

gaggagcagg ccgacgccca ctccaccctg gccaagatca gagtgaagtt cagcaggagc      180

gcagacgccc ccgcgtacca gcagggccag aaccagctct ataacgagct caatctagga      240

cgaagagagg agtacgatgt tttggacaag agacgtggcc gggaccctga gatgggggga      300

aagccgagaa ggaagaaccc tcaggaaggc ctgtacaatg aactgcagaa agataagatg      360

gcggaggcct acagtgagat tgggatgaaa ggcgagcgcc ggaggggcaa ggggcacgat      420

ggcctttacc agggactgag caccgccaca aaggatacct acgacgccct gcacatgcag      480

gcccttccac ctagagctga aggcagaggc agccttctga catgtggcga cgtggaagag      540

aaccccggac ccatgggcac cagcctgctg tgctggatgg ccctgtgcct gctgggcgcc      600

gaccacgccg atgcctgccc ctacagcaac cccagcctgt gcagcggagg cggcggcagc      660

gagctgccca cccagggcac cttctccaac gtgtccacca acgtgagccc agccaagccc      720

accaccaccg cctgtcctta ttccaatcct tccctgtgta gcggaggggg aggcagccca      780

gcccccagac ctcccacccc agcccccacc atcgccagcc agcctctgag cctgagaccc      840

gaggcctgcc gcccagccgc cggcggcgcc gtgcacacca gaggcctgga tttcgcctgc      900

gatatctaca tctgggcccc actggccggc acctgtggcg tgctgctgct gagcctggtg      960

atcaccctgt actgcaacca ccgcaaccgc aggcgcgtgt gcaagtgccc caggcccgtg     1020

gtgagagccg agggcagagg cagcctgctg acctgcggcg acgtggagga gaacccaggc     1080

ccatggagac cgacaccctg ctgctgtggg tgctgctgct gtgggtgcca ggcagcaccg     1140

gcagcgtgct gcacctggtg cccatcaacg ccaccagcaa ggacgactct gatgtgaccg     1200

aggtgatgtg gcagccagcc ctgagacggg gcagaggcct gcaggcccag ggctacggcg     1260

tgagaatcca ggacgctggc gtgtacctgc tgtactccca ggtgctgttc caggacgtga     1320

ccttcacaat gggccaggtg gtgagccggg agggccaggg cagacaggag accctgttcc     1380

ggtgcatccg gagcatgccc agccaccccg acagagccta caacagctgc tacagcgctg     1440

gcgtgtttca cctgcaccag ggcgacatcc tgagcgtgat catccccaga gccagagcca     1500

agctgaacct gtccccccac ggcacctttc tgggcttcgt gaagctgtct ggaggcggct     1560

cggatcccgc cgagcccaaa tctcctgaca aaactcacac atgcccaccg tgcccaaaag     1620

atcccaaatt ttgggtgctg gtggtggttg gtggagtcct ggcttgctat agcttgctag     1680

taacagtggc ctttattatt ttctgggtga ggagtaagag gagcaggctc ctgcacagtg     1740

actacatgaa catgactccc cgccgccccg ggcccacccg caagcattac cagccctatg     1800

ccccaccacg cgacttcgca gcctatcgct cctctggggg agggggatct atgagcagac     1860

tggacaagag caaagtgatc aacagcgccc tggaactgct gaacgaagtg ggcatcgagg     1920

gcctgaccac acggaagctg gctcagaaac tgggcgtgga acagcccacc ctgtactggc     1980

acgtgaagaa caagagagcc ctgctggacg ccctggctat cgagatgctg gaccggcacc     2040

acacccactt ttgcccactg gaaggcgaga gctggcagga cttcctgcgg aacaacgcca     2100

agagcttcag atgtgccctg ctgagccacc gggatggcgc caaagtgcac ctgggcacca     2160

gacctaccga gaagcagtat gagacactgg aaaaccagct ggcattcctg tgccagcagg     2220

gattcagcct ggaaaacgcc ctgtacgccc tgagcgccgt gggccacttt acactgggat     2280

gcgtgctgga agatcaggaa caccaggtgg ccaaagagga aagagagaca cccaccaccg     2340

acagcatgcc ccctctgctg agacaggcca tcgagctgtt cgatcatcaa ggcgccgagc     2400

ccgccttcct gttcggactg gaactgatca tctgcgggct ggaaaagcag ctgaagtgcg     2460

agagcggctc c                                                          2471


<210>  27
<211>  2471
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  APRIL CAR system, TIP-z-2A-SP-APRIL-HNG-TM-OX40-CD28-L-TetRB

<400>  27
atgtggacct ggaacgccta cgcctttgcc gcccctagcg gaggcggatc tagagtgaag       60

ttcagcagga gcgcagacgc ccccgcgtac cagcagggcc agaaccagct ctataacgag      120

ctcaatctag gacgaagaga ggagtacgat gttttggaca agagacgtgg ccgggaccct      180

gagatggggg gaaagccgag aaggaagaac cctcaggaag gcctgtacaa tgaactgcag      240

aaagataaga tggcggaggc ctacagtgag attgggatga aaggcgagcg ccggaggggc      300

aaggggcacg atggccttta ccagggactg agcaccgcca caaaggatac ctacgacgcc      360

ctgcacatgc aggcccttcc acctagagct gaaggcagag gcagccttct gacatgtggc      420

gacgtggaag agaaccccgg acccatgggc accagcctgc tgtgctggat ggccctgtgc      480

ctgctgggcg ccgaccacgc cgatgcctgc ccctacagca accccagcct gtgcagcgga      540

ggcggcggca gcgagctgcc cacccagggc accttctcca acgtgtccac caacgtgagc      600

ccagccaagc ccaccaccac cgcctgtcct tattccaatc cttccctgtg tagcggaggg      660

ggaggcagcc cagcccccag acctcccacc ccagccccca ccatcgccag ccagcctctg      720

agcctgagac ccgaggcctg ccgcccagcc gccggcggcg ccgtgcacac cagaggcctg      780

gatttcgcct gcgatatcta catctgggcc ccactggccg gcacctgtgg cgtgctgctg      840

ctgagcctgg tgatcaccct gtactgcaac caccgcaacc gcaggcgcgt gtgcaagtgc      900

cccaggcccg tggtgagagc cgagggcaga ggcagcctgc tgacctgcgg cgacgtggag      960

gagaacccag gcccatggag accgacaccc tgctgctgtg ggtgctgctg ctgtgggtgc     1020

caggcagcac cggcagcgtg ctgcacctgg tgcccatcaa cgccaccagc aaggacgact     1080

ctgatgtgac cgaggtgatg tggcagccag ccctgagacg gggcagaggc ctgcaggccc     1140

agggctacgg cgtgagaatc caggacgctg gcgtgtacct gctgtactcc caggtgctgt     1200

tccaggacgt gaccttcaca atgggccagg tggtgagccg ggagggccag ggcagacagg     1260

agaccctgtt ccggtgcatc cggagcatgc ccagccaccc cgacagagcc tacaacagct     1320

gctacagcgc tggcgtgttt cacctgcacc agggcgacat cctgagcgtg atcatcccca     1380

gagccagagc caagctgaac ctgtcccccc acggcacctt tctgggcttc gtgaagctgt     1440

ctggaggcgg ctcggatccc gccgagccca aatctcctga caaaactcac acatgcccac     1500

cgtgcccaaa agatcccaaa ttttgggtgc tggtggtggt tggtggagtc ctggcttgct     1560

atagcttgct agtaacagtg gcctttatta ttttctgggt gaggagtaag aggagcaggc     1620

tcctgcacag tgactacatg aacatgactc cccgccgccc cgggcccacc cgcaagcatt     1680

accagcccta tgccccacca cgcgacttcg cagcctatcg ctccagggac cagaggctgc     1740

cccccgatgc ccacaagccc cctgggggag gcagtttccg gacccccatc caagaggagc     1800

aggccgacgc ccactccacc ctggccaaga tctctggggg agggggatct atgagcagac     1860

tggacaagag caaagtgatc aacagcgccc tggaactgct gaacgaagtg ggcatcgagg     1920

gcctgaccac acggaagctg gctcagaaac tgggcgtgga acagcccacc ctgtactggc     1980

acgtgaagaa caagagagcc ctgctggacg ccctggctat cgagatgctg gaccggcacc     2040

acacccactt ttgcccactg gaaggcgaga gctggcagga cttcctgcgg aacaacgcca     2100

agagcttcag atgtgccctg ctgagccacc gggatggcgc caaagtgcac ctgggcacca     2160

gacctaccga gaagcagtat gagacactgg aaaaccagct ggcattcctg tgccagcagg     2220

gattcagcct ggaaaacgcc ctgtacgccc tgagcgccgt gggccacttt acactgggat     2280

gcgtgctgga agatcaggaa caccaggtgg ccaaagagga aagagagaca cccaccaccg     2340

acagcatgcc ccctctgctg agacaggcca tcgagctgtt cgatcatcaa ggcgccgagc     2400

ccgccttcct gttcggactg gaactgatca tctgcgggct ggaaaagcag ctgaagtgcg     2460

agagcggctc c                                                          2471


<210>  28
<211>  2471
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  APRIL CAR system, TIP-CD28-OX40-z-2A-SP-APRIL-HNG-TM-L-TetRB

<400>  28
atgtggacct ggaacgccta cgcctttgcc gcccctagcg gaggcggatc taggagtaag       60

aggagcaggc tcctgcacag tgactacatg aacatgactc cccgccgccc cgggcccacc      120

cgcaagcatt accagcccta tgccccacca cgcgacttcg cagcctatcg ctccagggac      180

cagaggctgc cccccgatgc ccacaagccc cctgggggag gcagtttccg gacccccatc      240

caagaggagc aggccgacgc ccactccacc ctggccaaga tcagagtgaa gttcagcagg      300

agcgcagacg cccccgcgta ccagcagggc cagaaccagc tctataacga gctcaatcta      360

ggacgaagag aggagtacga tgttttggac aagagacgtg gccgggaccc tgagatgggg      420

ggaaagccga gaaggaagaa ccctcaggaa ggcctgtaca atgaactgca gaaagataag      480

atggcggagg cctacagtga gattgggatg aaaggcgagc gccggagggg caaggggcac      540

gatggccttt accagggact gagcaccgcc acaaaggata cctacgacgc cctgcacatg      600

caggcccttc cacctagagc tgaaggcaga ggcagccttc tgacatgtgg cgacgtggaa      660

gagaaccccg gacccatggg caccagcctg ctgtgctgga tggccctgtg cctgctgggc      720

gccgaccacg ccgatgcctg cccctacagc aaccccagcc tgtgcagcgg aggcggcggc      780

agcgagctgc ccacccaggg caccttctcc aacgtgtcca ccaacgtgag cccagccaag      840

cccaccacca ccgcctgtcc ttattccaat ccttccctgt gtagcggagg gggaggcagc      900

ccagccccca gacctcccac cccagccccc accatcgcca gccagcctct gagcctgaga      960

cccgaggcct gccgcccagc cgccggcggc gccgtgcaca ccagaggcct ggatttcgcc     1020

tgcgatatct acatctgggc cccactggcc ggcacctgtg gcgtgctgct gctgagcctg     1080

gtgatcaccc tgtactgcaa ccaccgcaac cgcaggcgcg tgtgcaagtg ccccaggccc     1140

gtggtgagag ccgagggcag aggcagcctg ctgacctgcg gcgacgtgga ggagaaccca     1200

ggcccatgga gaccgacacc ctgctgctgt gggtgctgct gctgtgggtg ccaggcagca     1260

ccggcagcgt gctgcacctg gtgcccatca acgccaccag caaggacgac tctgatgtga     1320

ccgaggtgat gtggcagcca gccctgagac ggggcagagg cctgcaggcc cagggctacg     1380

gcgtgagaat ccaggacgct ggcgtgtacc tgctgtactc ccaggtgctg ttccaggacg     1440

tgaccttcac aatgggccag gtggtgagcc gggagggcca gggcagacag gagaccctgt     1500

tccggtgcat ccggagcatg cccagccacc ccgacagagc ctacaacagc tgctacagcg     1560

ctggcgtgtt tcacctgcac cagggcgaca tcctgagcgt gatcatcccc agagccagag     1620

ccaagctgaa cctgtccccc cacggcacct ttctgggctt cgtgaagctg tctggaggcg     1680

gctcggatcc cgccgagccc aaatctcctg acaaaactca cacatgccca ccgtgcccaa     1740

aagatcccaa attttgggtg ctggtggtgg ttggtggagt cctggcttgc tatagcttgc     1800

tagtaacagt ggcctttatt attttctggg tgtctggggg agggggatct atgagcagac     1860

tggacaagag caaagtgatc aacagcgccc tggaactgct gaacgaagtg ggcatcgagg     1920

gcctgaccac acggaagctg gctcagaaac tgggcgtgga acagcccacc ctgtactggc     1980

acgtgaagaa caagagagcc ctgctggacg ccctggctat cgagatgctg gaccggcacc     2040

acacccactt ttgcccactg gaaggcgaga gctggcagga cttcctgcgg aacaacgcca     2100

agagcttcag atgtgccctg ctgagccacc gggatggcgc caaagtgcac ctgggcacca     2160

gacctaccga gaagcagtat gagacactgg aaaaccagct ggcattcctg tgccagcagg     2220

gattcagcct ggaaaacgcc ctgtacgccc tgagcgccgt gggccacttt acactgggat     2280

gcgtgctgga agatcaggaa caccaggtgg ccaaagagga aagagagaca cccaccaccg     2340

acagcatgcc ccctctgctg agacaggcca tcgagctgtt cgatcatcaa ggcgccgagc     2400

ccgccttcct gttcggactg gaactgatca tctgcgggct ggaaaagcag ctgaagtgcg     2460

agagcggctc c                                                          2471


<210>  29
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  29

Gly Gly Gly Ser 
1               


<210>  30
<211>  416
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  trimeric APRIL CAR antigen binding domain

<400>  30

Ser Val Leu His Leu Val Pro Ile Asn Ala Thr Ser Lys Asp Asp Ser 
1               5                   10                  15      


Asp Val Thr Glu Val Met Trp Gln Pro Ala Leu Arg Arg Gly Arg Gly 
            20                  25                  30          


Leu Gln Ala Gln Gly Tyr Gly Val Arg Ile Gln Asp Ala Gly Val Tyr 
        35                  40                  45              


Leu Leu Tyr Ser Gln Val Leu Phe Gln Asp Val Thr Phe Thr Met Gly 
    50                  55                  60                  


Gln Val Val Ser Arg Glu Gly Gln Gly Arg Gln Glu Thr Leu Phe Arg 
65                  70                  75                  80  


Cys Ile Arg Ser Met Pro Ser His Pro Asp Arg Ala Tyr Asn Ser Cys 
                85                  90                  95      


Tyr Ser Ala Gly Val Phe His Leu His Gln Gly Asp Ile Leu Ser Val 
            100                 105                 110         


Ile Ile Pro Arg Ala Arg Ala Lys Leu Asn Leu Ser Pro His Gly Thr 
        115                 120                 125             


Phe Leu Gly Phe Val Lys Leu Ser Gly Gly Gly Ser Ser Val Leu His 
    130                 135                 140                 


Leu Val Pro Ile Asn Ala Thr Ser Lys Asp Asp Ser Asp Val Thr Glu 
145                 150                 155                 160 


Val Met Trp Gln Pro Ala Leu Arg Arg Gly Arg Gly Leu Gln Ala Gln 
                165                 170                 175     


Gly Tyr Gly Val Arg Ile Gln Asp Ala Gly Val Tyr Leu Leu Tyr Ser 
            180                 185                 190         


Gln Val Leu Phe Gln Asp Val Thr Phe Thr Met Gly Gln Val Val Ser 
        195                 200                 205             


Arg Glu Gly Gln Gly Arg Gln Glu Thr Leu Phe Arg Cys Ile Arg Ser 
    210                 215                 220                 


Met Pro Ser His Pro Asp Arg Ala Tyr Asn Ser Cys Tyr Ser Ala Gly 
225                 230                 235                 240 


Val Phe His Leu His Gln Gly Asp Ile Leu Ser Val Ile Ile Pro Arg 
                245                 250                 255     


Ala Arg Ala Lys Leu Asn Leu Ser Pro His Gly Thr Phe Leu Gly Phe 
            260                 265                 270         


Val Lys Leu Ser Gly Gly Gly Ser Ser Val Leu His Leu Val Pro Ile 
        275                 280                 285             


Asn Ala Thr Ser Lys Asp Asp Ser Asp Val Thr Glu Val Met Trp Gln 
    290                 295                 300                 


Pro Ala Leu Arg Arg Gly Arg Gly Leu Gln Ala Gln Gly Tyr Gly Val 
305                 310                 315                 320 


Arg Ile Gln Asp Ala Gly Val Tyr Leu Leu Tyr Ser Gln Val Leu Phe 
                325                 330                 335     


Gln Asp Val Thr Phe Thr Met Gly Gln Val Val Ser Arg Glu Gly Gln 
            340                 345                 350         


Gly Arg Gln Glu Thr Leu Phe Arg Cys Ile Arg Ser Met Pro Ser His 
        355                 360                 365             


Pro Asp Arg Ala Tyr Asn Ser Cys Tyr Ser Ala Gly Val Phe His Leu 
    370                 375                 380                 


His Gln Gly Asp Ile Leu Ser Val Ile Ile Pro Arg Ala Arg Ala Lys 
385                 390                 395                 400 


Leu Asn Leu Ser Pro His Gly Thr Phe Leu Gly Phe Val Lys Leu Ser 
                405                 410                 415     


<210>  31
<211>  48
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8 stalk sequence, CD8STK

<400>  31

Asp Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr 
1               5                   10                  15      


Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala 
            20                  25                  30          


Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile 
        35                  40                  45              


<210>  32
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 transmembrane domain

<400>  32

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25          


<210>  33
<211>  189
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28-OX40-CD3z endodomain

<400>  33

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Asp Gln Arg Leu Pro Pro 
        35                  40                  45              


Asp Ala His Lys Pro Pro Gly Gly Gly Ser Phe Arg Thr Pro Ile Gln 
    50                  55                  60                  


Glu Glu Gln Ala Asp Ala His Ser Thr Leu Ala Lys Ile Arg Val Lys 
65                  70                  75                  80  


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
                85                  90                  95      


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
            100                 105                 110         


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
        115                 120                 125             


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
    130                 135                 140                 


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
145                 150                 155                 160 


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
                165                 170                 175     


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            180                 185                 


<210>  34
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mannose-binding protein A: parallel homotrimer

<400>  34

Ala Ile Glu Val Lys Leu Ala Asn Met Glu Ala Glu Ile Asn Thr Leu 
1               5                   10                  15      


Lys Ser Lys Leu Glu Leu Thr Asn Lys Leu His Ala Phe Ser Met 
            20                  25                  30      


<210>  35
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Coiled-coil serine-rich protein 1: parallel homotrimer

<400>  35

Glu Trp Glu Ala Leu Glu Lys Lys Leu Ala Ala Leu Glu Ser Lys Leu 
1               5                   10                  15      


Gln Ala Leu Glu Lys Lys Leu Glu Ala Leu Glu His Gly 
            20                  25                  


<210>  36
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide release factor 2: anti-parallel heterotrimer. Chain A

<400>  36

Ile Asn Pro Val Asn Asn Arg Ile Gln Asp Leu Thr Glu Arg Ser Asp 
1               5                   10                  15      


Val Leu Arg Gly Tyr Leu Asp Tyr 
            20                  


<210>  37
<211>  51
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide release factor 2: anti-parallel heterotrimer. Chain B

<400>  37

Val Val Asp Thr Leu Asp Gln Met Lys Gln Gly Leu Glu Asp Val Ser 
1               5                   10                  15      


Gly Leu Leu Glu Leu Ala Val Glu Ala Asp Asp Glu Glu Thr Phe Asn 
            20                  25                  30          


Glu Ala Val Ala Glu Leu Asp Ala Leu Glu Glu Lys Leu Ala Gln Leu 
        35                  40                  45              


Glu Phe Arg 
    50      


<210>  38
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tenascin C

<400>  38

Ala Cys Gly Cys Ala Ala Ala Pro Asp Val Lys Asp Leu Leu Ser Arg 
1               5                   10                  15      


Leu Glu Glu Leu Glu Gly Leu Val Ser Ser Leu Arg Glu Gln 
            20                  25                  30  


<210>  39
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  furin cleavage site

<400>  39

Lys Gln Lys Lys Gln Lys 
1               5       


<210>  40
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TiP sequence, as attached at a protein amino-terminus

<400>  40

Trp Thr Trp Asn Ala Tyr Ala Phe Ala Ala Pro Ser Gly Gly Gly Ser 
1               5                   10                  15      


