                         SEQUENCE LISTING

<110>  Evolva
       River Stone Biotech ApS
 
<120>  NORCOCLAURINE SYNTHASES WITH INCREASED ACTIVITY

<130>  P1349WO00

<150>  PA201770474
<151>  2017-06-16

<150>  PA201770533
<151>  2017-06-30

<160>  21    

<170>  PatentIn version 3.5

<210>  1
<211>  196
<212>  PRT
<213>  Coptis japonica

<400>  1

Met Arg Met Glu Val Val Leu Val Val Phe Leu Met Phe Ile Gly Thr 
1               5                   10                  15      


Ile Asn Cys Glu Arg Leu Ile Phe Asn Gly Arg Pro Leu Leu His Arg 
            20                  25                  30          


Val Thr Lys Glu Glu Thr Val Met Leu Tyr His Glu Leu Glu Val Ala 
        35                  40                  45              


Ala Ser Ala Asp Glu Val Trp Ser Val Glu Gly Ser Pro Glu Leu Gly 
    50                  55                  60                  


Leu His Leu Pro Asp Leu Leu Pro Ala Gly Ile Phe Ala Lys Phe Glu 
65                  70                  75                  80  


Ile Thr Gly Asp Gly Gly Glu Gly Ser Ile Leu Asp Met Thr Phe Pro 
                85                  90                  95      


Pro Gly Gln Phe Pro His His Tyr Arg Glu Lys Phe Val Phe Phe Asp 
            100                 105                 110         


His Lys Asn Arg Tyr Lys Leu Val Glu Gln Ile Asp Gly Asp Phe Phe 
        115                 120                 125             


Asp Leu Gly Val Thr Tyr Tyr Met Asp Thr Ile Arg Val Val Ala Thr 
    130                 135                 140                 


Gly Pro Asp Ser Cys Val Ile Lys Ser Thr Thr Glu Tyr His Val Lys 
145                 150                 155                 160 


Pro Glu Phe Ala Lys Ile Val Lys Pro Leu Ile Asp Thr Val Pro Leu 
                165                 170                 175     


Ala Ile Met Ser Glu Ala Ile Ala Lys Val Val Leu Glu Asn Lys His 
            180                 185                 190         


Lys Ser Ser Glu 
        195     


<210>  2
<211>  591
<212>  DNA
<213>  Coptis japonica

<400>  2
atgaggatgg aagttgttct agttgttttc ttgatgttca taggtacgat aaattgtgaa       60

agattgatat tcaatggacg accgctactc catcgcgtaa caaaagagga gactgtaatg      120

ctttatcatg agctggaagt agctgcttca gccgatgaag tgtggagtgt cgaaggttcg      180

cctgagttgg gcttgcattt gcctgacttg ctccctgctg gtatatttgc aaagtttgaa      240

attactggtg atggaggtga aggttcgatc ctggacatga cattcccccc aggtcagttt      300

ccacatcatt acagggagaa gttcgtgttc ttcgatcaca agaatcgtta caagttagta      360

gaacagatcg atggtgattt tttcgatcta ggtgttacat actatatgga tacaatccga      420

gttgttgcga caggccctga ttcatgtgtc atcaagtcta ctactgaata ccatgtgaaa      480

cctgagtttg ccaaaatcgt caaaccactt attgacactg ttccactagc tatcatgtct      540

gaagcgattg caaaggttgt tctagagaac aaacacaaga gttcagagta a               591


<210>  3
<211>  591
<212>  DNA
<213>  Coptis japonica

<400>  3
atgagaatgg aagtcgtctt ggtcgttttc ttgatgttca ttggtactat caactgcgaa       60

agattgatct tcaatggtag acctttgttg cacagagtta ccaaagaaga aaccgttatg      120

ttgtaccacg aattggaagt tgctgcttct gctgatgaag tttggtctgt tgaaggttct      180

ccagaattgg gtttacattt gccagatttg ttgccagctg gtatttttgc caagttcgaa      240

attactggtg atggtggtga aggttccatt ttggatatga cttttccacc aggtcaattc      300

ccacatcatt acagagaaaa gttcgtcttt ttcgaccaca agaacagata caagttggtc      360

gaacaaatcg atggtgattt cttcgatttg ggtgttactt actacatgga caccattaga      420

gttgttgcta ctggtccaga ttcttgcgtt attaagtcta ctactgaata ccacgtcaag      480

ccagaatttg ctaaaatcgt taagccattg atcgataccg ttccattggc tattatgtct      540

gaagctattg ccaaggttgt cttggaaaac aaacacaagt catctgaatg a               591


<210>  4
<211>  6
<212>  PRT
<213>  Coptis japonica

<400>  4

Pro Ala Gly Ile Phe Ala 
1               5       


<210>  5
<211>  11
<212>  PRT
<213>  Coptis japonica

<400>  5

Thr Val Pro Leu Ala Ile Met Ser Glu Ala Ile 
1               5                   10      


<210>  6
<211>  499
<212>  PRT
<213>  Beta vulgaris

<400>  6

Met Asp His Ala Thr Leu Ala Met Ile Leu Ala Ile Leu Phe Ile Ser 
1               5                   10                  15      


Phe His Phe Ile Lys Leu Leu Phe Ser Gln Gln Thr Thr Lys Leu Leu 
            20                  25                  30          


Pro Pro Gly Pro Lys Pro Leu Pro Ile Ile Gly Asn Ile Leu Glu Val 
        35                  40                  45              


Gly Lys Lys Pro His Arg Ser Phe Ala Asn Leu Ala Lys Ile His Gly 
    50                  55                  60                  


Pro Leu Ile Ser Leu Arg Leu Gly Ser Val Thr Thr Ile Val Val Ser 
65                  70                  75                  80  


Ser Ala Asp Val Ala Lys Glu Met Phe Leu Lys Lys Asp His Pro Leu 
                85                  90                  95      


Ser Asn Arg Thr Ile Pro Asn Ser Val Thr Ala Gly Asp His His Lys 
            100                 105                 110         


Leu Thr Met Ser Trp Leu Pro Val Ser Pro Lys Trp Arg Asn Phe Arg 
        115                 120                 125             


Lys Ile Thr Ala Val His Leu Leu Ser Pro Gln Arg Leu Asp Ala Cys 
    130                 135                 140                 


Gln Thr Phe Arg His Ala Lys Val Gln Gln Leu Tyr Glu Tyr Val Gln 
145                 150                 155                 160 


Glu Cys Ala Gln Lys Gly Gln Ala Val Asp Ile Gly Lys Ala Ala Phe 
                165                 170                 175     


Thr Thr Ser Leu Asn Leu Leu Ser Lys Leu Phe Phe Ser Val Glu Leu 
            180                 185                 190         


Ala His His Lys Ser His Thr Ser Gln Glu Phe Lys Glu Leu Ile Trp 
        195                 200                 205             


Asn Ile Met Glu Asp Ile Gly Lys Pro Asn Tyr Ala Asp Tyr Phe Pro 
    210                 215                 220                 


Ile Leu Gly Cys Val Asp Pro Ser Gly Ile Arg Arg Arg Leu Ala Cys 
225                 230                 235                 240 


Ser Phe Asp Lys Leu Ile Ala Val Phe Gln Gly Ile Ile Cys Glu Arg 
                245                 250                 255     


Leu Ala Pro Asp Ser Ser Thr Thr Thr Thr Thr Thr Thr Asp Asp Val 
            260                 265                 270         


Leu Asp Val Leu Leu Gln Leu Phe Lys Gln Asn Glu Leu Thr Met Gly 
        275                 280                 285             


Glu Ile Asn His Leu Leu Val Asp Ile Phe Asp Ala Gly Thr Asp Thr 
    290                 295                 300                 


Thr Ser Ser Thr Leu Glu Trp Val Met Thr Glu Leu Ile Arg Asn Pro 
305                 310                 315                 320 


Glu Met Met Glu Lys Ala Gln Glu Glu Ile Lys Gln Val Leu Gly Lys 
                325                 330                 335     


Asp Lys Gln Ile Gln Glu Ser Asp Ile Ile Asn Leu Pro Tyr Leu Gln 
            340                 345                 350         


Ala Ile Ile Lys Glu Thr Leu Arg Leu His Pro Pro Thr Val Phe Leu 
        355                 360                 365             


Leu Pro Arg Lys Ala Asp Thr Asp Val Glu Leu Tyr Gly Tyr Ile Val 
    370                 375                 380                 


Pro Lys Asp Ala Gln Ile Leu Val Asn Leu Trp Ala Ile Gly Arg Asp 
385                 390                 395                 400 


Pro Asn Ala Trp Gln Asn Ala Asp Ile Phe Ser Pro Glu Arg Phe Ile 
                405                 410                 415     


Gly Cys Glu Ile Asp Val Lys Gly Arg Asp Phe Gly Leu Leu Pro Phe 
            420                 425                 430         


Gly Ala Gly Arg Arg Ile Cys Pro Gly Met Asn Leu Ala Ile Arg Met 
        435                 440                 445             


Leu Thr Leu Met Leu Ala Thr Leu Leu Gln Phe Phe Asn Trp Lys Leu 
    450                 455                 460                 


Glu Gly Asp Ile Ser Pro Lys Asp Leu Asp Met Asp Glu Lys Phe Gly 
465                 470                 475                 480 


Ile Ala Leu Gln Lys Thr Lys Pro Leu Lys Leu Ile Pro Ile Pro Arg 
                485                 490                 495     


Tyr Gly Ser 
            


<210>  7
<211>  1500
<212>  DNA
<213>  Beta vulgaris

<400>  7
atggatcatg ctaccttggc tatgattttg gccatcttgt tcattagctt ccacttcatc       60

aagctgttgt tctctcaaca aactaccaag ttgttgccac caggtccaaa accattgcca      120

attattggta acatcttgga ggttggtaag aagccacata gatcttttgc taatttggcc      180

aagattcacg gtccattgat ttctttgaga ttgggttctg ttacgaccat cgttgtttct      240

tcagctgatg ttgctaaaga gatgttcttg aagaaggatc acccattgtc caacagaact      300

attccaaatt ctgttaccgc tggtgatcat cataagttga ctatgtcttg gttgccagtt      360

tctccaaagt ggcgtaattt cagaaagatt accgctgttc atttgttgtc cccacaaaga      420

ttggatgctt gtcaaacttt tagacacgct aaggttcaac agttgtacga atacgttcaa      480

gaatgtgctc aaaaaggtca agccgttgat attggtaaag ctgcttttac tacctccttg      540

aacttgctgt ctaagttgtt cttctctgtt gaattggctc atcacaagtc tcatacctct      600

caagaattca aagagctgat ctggaacatc atggaagata tcggtaagcc aaattacgct      660

gattacttcc caattttggg ttgcgttgat ccatctggta ttagaagaag attggcttgc      720

tctttcgata agttgattgc tgttttccaa ggtatcatct gtgaaagatt agccccagat      780

tcttctacta ctacaactac taccactgat gatgttttgg atgtgttgtt gcagttgttc      840

aagcaaaacg aattgaccat gggtgaaatc aaccacttgt tggttgatat tttcgatgct      900

ggtactgata ccacttcctc tactttggaa tgggttatga ccgaattgat cagaaaccca      960

gaaatgatgg aaaaggccca agaagagatt aagcaagttt tgggtaaaga caagcagatc     1020

caagaatccg atattatcaa cttgccatac ttgcaggcca tcatcaaaga aacattgaga     1080

ttgcatccac caaccgtttt tttgttgcca agaaaagctg ataccgatgt tgagttgtat     1140

ggttacatcg ttccaaagga tgcccaaatc ttggttaatt tgtgggctat tggtagagat     1200

ccaaatgctt ggcaaaacgc cgatattttc tcaccagaaa ggttcattgg ttgcgaaatt     1260

gatgttaagg gtagagactt tggtttgttg ccttttggtg ctggtagaag gatttgtcca     1320

ggtatgaatt tggctatcag aatgttgact ttgatgctgg ctactctgtt gcaatttttc     1380

aactggaaat tggagggtga catctcacca aaagatttgg atatggacga aaagttcggt     1440

atcgccttgc aaaaaactaa gccattgaag ttgatcccca ttcctagata cggttcttga     1500


<210>  8
<211>  470
<212>  PRT
<213>  Pseudomonas putida

<400>  8

Met Thr Pro Glu Gln Phe Arg Gln Tyr Gly His Gln Leu Ile Asp Leu 
1               5                   10                  15      


Ile Ala Asp Tyr Arg Gln Thr Val Gly Glu Arg Pro Val Met Ala Gln 
            20                  25                  30          


Val Glu Pro Gly Tyr Leu Lys Ala Ala Leu Pro Ala Thr Ala Pro Gln 
        35                  40                  45              


Gln Gly Glu Pro Phe Ala Ala Ile Leu Asp Asp Val Asn Asn Leu Val 
    50                  55                  60                  


Met Pro Gly Leu Ser His Trp Gln His Pro Asp Phe Tyr Gly Tyr Phe 
65                  70                  75                  80  


Pro Ser Asn Gly Thr Leu Ser Ser Val Leu Gly Asp Phe Leu Ser Thr 
                85                  90                  95      


Gly Leu Gly Val Leu Gly Leu Ser Trp Gln Ser Ser Pro Ala Leu Ser 
            100                 105                 110         


Glu Leu Glu Glu Thr Thr Leu Asp Trp Leu Arg Gln Leu Leu Gly Leu 
        115                 120                 125             


Ser Gly Gln Trp Ser Gly Val Ile Gln Asp Thr Ala Ser Thr Ser Thr 
    130                 135                 140                 


Leu Val Ala Leu Ile Ser Ala Arg Glu Arg Ala Thr Asp Tyr Ala Leu 
145                 150                 155                 160 


Val Arg Gly Gly Leu Gln Ala Glu Pro Lys Pro Leu Ile Val Tyr Val 
                165                 170                 175     


Ser Ala His Ala His Ser Ser Val Asp Lys Ala Ala Leu Leu Ala Gly 
            180                 185                 190         


Phe Gly Arg Asp Asn Ile Arg Leu Ile Pro Thr Asp Glu Arg Tyr Ala 
        195                 200                 205             


Leu Arg Pro Glu Ala Leu Gln Ala Ala Ile Glu Gln Asp Leu Ala Ala 
    210                 215                 220                 


Gly Asn Gln Pro Cys Ala Val Val Ala Thr Thr Gly Thr Thr Thr Thr 
225                 230                 235                 240 


Thr Ala Leu Asp Pro Leu Arg Pro Val Gly Glu Ile Ala Gln Ala Asn 
                245                 250                 255     


Gly Leu Trp Leu His Val Asp Ser Ala Met Ala Gly Ser Ala Met Ile 
            260                 265                 270         


Leu Pro Glu Cys Arg Trp Met Trp Asp Gly Ile Glu Leu Ala Asp Ser 
        275                 280                 285             


Val Val Val Asn Ala His Lys Trp Leu Gly Val Ala Phe Asp Cys Ser 
    290                 295                 300                 


Ile Tyr Tyr Val Arg Asp Pro Gln His Leu Ile Arg Val Met Ser Thr 
305                 310                 315                 320 


Asn Pro Ser Tyr Leu Gln Ser Ala Val Asp Gly Glu Val Lys Asn Leu 
                325                 330                 335     


Arg Asp Trp Gly Ile Pro Leu Gly Arg Arg Phe Arg Ala Leu Lys Leu 
            340                 345                 350         


Trp Phe Met Leu Arg Ser Glu Gly Val Asp Ala Leu Gln Ala Arg Leu 
        355                 360                 365             


Arg Arg Asp Leu Asp Asn Ala Gln Trp Leu Ala Gly Gln Val Glu Ala 
    370                 375                 380                 


Ala Ala Glu Trp Glu Val Leu Ala Pro Val Gln Leu Gln Thr Leu Cys 
385                 390                 395                 400 


Ile Arg His Arg Pro Ala Gly Leu Glu Gly Glu Ala Leu Asp Ala His 
                405                 410                 415     


Thr Lys Gly Trp Ala Glu Arg Leu Asn Ala Ser Gly Ala Ala Tyr Val 
            420                 425                 430         


Thr Pro Ala Thr Leu Asp Gly Arg Trp Met Val Arg Val Ser Ile Gly 
        435                 440                 445             


Ala Leu Pro Thr Glu Arg Gly Asp Val Gln Arg Leu Trp Ala Arg Leu 
    450                 455                 460                 


Gln Asp Val Ile Lys Gly 
465                 470 


<210>  9
<211>  1413
<212>  DNA
<213>  Pseudomonas putida

<400>  9
atgaccccag aacaattcag acaatacggt caccaattga ttgatttgat cgccgattac       60

agacaaaccg ttggtgaaag accagttatg gctcaagttg aaccaggtta tttgaaagct      120

gctttgccag ctactgctcc acaacaaggt gaaccatttg ctgctatttt ggatgatgtt      180

aacaacttgg ttatgccagg tttgtctcat tggcaacatc cagattttta cggttacttt      240

ccatccaacg gtactttgtc atctgttttg ggtgatttct tgtctactgg tttgggtgtt      300

ttaggtttgt catggcaatc ttctccagct ttgtctgaat tggaagaaac tactttggat      360

tggttgagac agttgttggg tttatctggt caatggtctg gtgttattca agatactgct      420

tctacttcta ccttggttgc tttgatttct gctagagaaa gagctactga ttacgctttg      480

gttagaggtg gtttacaagc tgaacctaaa ccattgatcg tttacgtttc tgctcatgcc      540

cattcttcag ttgataaggc tgctttgttg gctggttttg gtagagataa cattagattg      600

attccaaccg acgaaagata cgctttaaga ccagaagcct tgcaagctgc tattgaacaa      660

gatttggctg ctggtaatca accatgtgct gttgttgcta ctactggtac tactactaca      720

actgctttgg atccattaag acctgtaggt gaaattgctc aagctaatgg tttgtggttg      780

catgttgatt cagctatggc tggttctgct atgattttgc cagaatgtag atggatgtgg      840

gatggtattg aattggctga ttctgttgtt gttaacgccc ataagtggtt gggtgttgct      900

tttgattgct ctatctacta cgttagagat ccacaacact tgatcagagt gatgtctact      960

aatccatcct acttgcaatc agctgttgat ggtgaagtta agaacttgag agattggggt     1020

attccattgg gtagaagatt tagagctttg aagttgtggt ttatgttgag atccgaaggt     1080

gttgatgcat tgcaagctag attgagaaga gatttggata atgctcaatg gttggctgga     1140

caagttgaag ctgctgctga atgggaagtt ttggctccag ttcaattgca aaccttgtgc     1200

attagacata gaccagcagg tttggaaggt gaagccttgg atgctcatac aaaaggttgg     1260

gctgaaagat tgaatgcttc tggtgctgct tatgttactc cagctacttt agatggaaga     1320

tggatggtta gagtttccat tggtgcttta ccaactgaaa gaggtgacgt tcaaagattg     1380

tgggctagat tgcaagatgt tatcaagggt tga                                  1413


<210>  10
<211>  370
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  10

Met Ser Glu Ser Pro Met Phe Ala Ala Asn Gly Met Pro Lys Val Asn 
1               5                   10                  15      


Gln Gly Ala Glu Glu Asp Val Arg Ile Leu Gly Tyr Asp Pro Leu Ala 
            20                  25                  30          


Ser Pro Ala Leu Leu Gln Val Gln Ile Pro Ala Thr Pro Thr Ser Leu 
        35                  40                  45              


Glu Thr Ala Lys Arg Gly Arg Arg Glu Ala Ile Asp Ile Ile Thr Gly 
    50                  55                  60                  


Lys Asp Asp Arg Val Leu Val Ile Val Gly Pro Cys Ser Ile His Asp 
65                  70                  75                  80  


Leu Glu Ala Ala Gln Glu Tyr Ala Leu Arg Leu Lys Lys Leu Ser Asp 
                85                  90                  95      


Glu Leu Lys Gly Asp Leu Ser Ile Ile Met Arg Ala Tyr Leu Glu Lys 
            100                 105                 110         


Pro Arg Thr Thr Val Gly Trp Lys Gly Leu Ile Asn Asp Pro Asp Val 
        115                 120                 125             


Asn Asn Thr Phe Asn Ile Asn Lys Gly Leu Gln Ser Ala Arg Gln Leu 
    130                 135                 140                 


Phe Val Asn Leu Thr Asn Ile Gly Leu Pro Ile Gly Ser Glu Met Leu 
145                 150                 155                 160 


Asp Thr Ile Ser Pro Gln Tyr Leu Ala Asp Leu Val Ser Phe Gly Ala 
                165                 170                 175     


Ile Gly Ala Arg Thr Thr Glu Ser Gln Leu His Arg Glu Leu Ala Ser 
            180                 185                 190         


Gly Leu Ser Phe Pro Val Gly Phe Lys Asn Gly Thr Asp Gly Thr Leu 
        195                 200                 205             


Asn Val Ala Val Asp Ala Cys Gln Ala Ala Ala His Ser His His Phe 
    210                 215                 220                 


Met Gly Val Thr Leu His Gly Val Ala Ala Ile Thr Thr Thr Lys Gly 
225                 230                 235                 240 


Asn Glu His Cys Phe Val Ile Leu Arg Gly Gly Lys Lys Gly Thr Asn 
                245                 250                 255     


Tyr Asp Ala Lys Ser Val Ala Glu Ala Lys Ala Gln Leu Pro Ala Gly 
            260                 265                 270         


Ser Asn Gly Leu Met Ile Asp Tyr Ser His Gly Asn Ser Asn Lys Asp 
        275                 280                 285             


Phe Arg Asn Gln Pro Lys Val Asn Asp Val Val Cys Glu Gln Ile Ala 
    290                 295                 300                 


Asn Gly Glu Asn Ala Ile Thr Gly Val Met Ile Glu Ser Asn Ile Asn 
305                 310                 315                 320 


Glu Gly Asn Gln Gly Ile Pro Ala Glu Gly Lys Ala Gly Leu Lys Tyr 
                325                 330                 335     


Gly Val Ser Ile Thr Asp Ala Cys Ile Gly Trp Glu Thr Thr Glu Asp 
            340                 345                 350         


Val Leu Arg Lys Leu Ala Ala Ala Val Arg Gln Arg Arg Glu Val Asn 
        355                 360                 365             


Lys Lys 
    370 


<210>  11
<211>  1113
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  11
atgagtgaat ctccaatgtt cgctgccaac ggcatgccaa aggtaaatca aggtgctgaa       60

gaagatgtca gaattttagg ttacgaccca ttagcttctc cagctctcct tcaagtgcaa      120

atcccagcca caccaacttc tttggaaact gccaagagag gtagaagaga agctatagat      180

attattaccg gtaaagacga cagagttctt gtcattgtcg gtccttgttc catccatgat      240

ctagaagccg ctcaagaata cgctttgaga ttaaagaaat tgtcagatga attaaaaggt      300

gatttatcca tcattatgag agcatacttg gagaagccaa gaacaaccgt cggctggaaa      360

ggtctaatta atgaccctga tgttaacaac actttcaaca tcaacaaggg tttgcaatcc      420

gctagacaat tgtttgtcaa cttgacaaat atcggtttgc caattggttc tgaaatgctt      480

gataccattt ctcctcaata cttggctgat ttggtctcct tcggtgccat tggtgccaga      540

accaccgaat ctcaactgca cagagaattg gcctccggtt tgtctttccc agttggtttc      600

aagaacggta ccgatggtac cttaaatgtt gctgtggatg cttgtcaagc cgctgctcat      660

tctcaccatt tcatgggtgt tactttgcat ggtgttgctg ctatcaccac tactaagggt      720

aacgaacact gcttcgttat tctaagaggt ggtaaaaagg gtaccaacta cgacgctaag      780

tccgttgcag aagctaaggc tcaattgcct gccggttcca acggtctaat gattgactac      840

tctcacggta actccaataa ggatttcaga aaccaaccaa aggtcaatga cgttgtttgt      900

gagcaaatcg ctaacggtga aaacgccatt accggtgtca tgattgaatc aaacatcaac      960

gaaggtaacc aaggcatccc agccgaaggt aaagccggct tgaaatatgg tgtttccatc     1020

actgatgctt gtataggttg ggaaactact gaagacgtct tgaggaaatt ggctgctgct     1080

gtcagacaaa gaagagaagt taacaagaaa tag                                  1113


<210>  12
<211>  196
<212>  PRT
<213>  Coptis japonica

<400>  12

Met Arg Met Glu Val Val Leu Val Val Phe Leu Met Phe Ile Gly Thr 
1               5                   10                  15      


Ile Asn Cys Glu Arg Leu Ile Phe Asn Gly Arg Pro Leu Leu His Arg 
            20                  25                  30          


Val Thr Lys Glu Glu Thr Val Met Leu Tyr His Glu Leu Glu Val Ala 
        35                  40                  45              


Ala Ser Ala Asp Glu Val Trp Ser Val Glu Gly Ser Pro Glu Leu Gly 
    50                  55                  60                  


Leu His Leu Pro Asp Leu Leu Pro Ala Gly Ile Phe Ala Lys Phe Glu 
65                  70                  75                  80  


Ile Thr Gly Asp Gly Gly Glu Gly Ser Ile Leu Asp Met Thr Phe Pro 
                85                  90                  95      


Pro Gly Gln Phe Pro His His Tyr Arg Glu Lys Phe Val Phe Phe Asp 
            100                 105                 110         


His Lys Asn Arg Tyr Lys Leu Val Glu Gln Ile Asp Gly Asp Phe Phe 
        115                 120                 125             


Asp Leu Gly Val Thr Tyr Tyr Met Asp Thr Ile Arg Val Val Ala Thr 
    130                 135                 140                 


Gly Pro Asp Ser Cys Val Ile Lys Ser Thr Thr Glu Tyr His Val Lys 
145                 150                 155                 160 


Pro Glu Phe Ala Lys Ile Val Lys Pro Leu Ile Asp Thr Val Pro Leu 
                165                 170                 175     


Ala Ile Met Ser Glu Ala Ile Ala Lys Val Val Leu Glu Asn Lys His 
            180                 185                 190         


Lys Ser Ser Glu 
        195     


<210>  13
<211>  591
<212>  DNA
<213>  Coptis japonica

<400>  13
atgagaatgg aagtcgtctt ggtcgttttc ttgatgttca ttggtactat caactgcgaa       60

agattgatct tcaatggtag acctttgttg cacagagtta ccaaagaaga aaccgttatg      120

ttgtaccacg aattggaagt tgctgcttct gctgatgaag tttggtctgt tgaaggttct      180

ccagaattgg gtttacattt gccagatttg ttgccagctg gtatttttgc caagttcgaa      240

attactggtg atggtggtga aggttccatt ttggatatga cttttccacc aggtcaattc      300

ccacatcatt acagagaaaa gttcgtcttt ttcgaccaca agaacagata caagttggtc      360

gaacaaatcg atggtgattt cttcgatttg ggtgttactt actacatgga caccattaga      420

gttgttgcta ctggtccaga ttcttgcgtt attaagtcta ctactgaata ccacgtcaag      480

ccagaatttg ctaaaatcgt taagccattg atcgataccg ttccattggc tattatgtct      540

gaagctattg ccaaggttgt cttggaaaac aaacacaagt catctgaatg a               591


<210>  14
<211>  178
<212>  PRT
<213>  Coptis japonica

<400>  14

Met Glu Arg Leu Ile Phe Asn Gly Arg Pro Leu Leu His Arg Val Thr 
1               5                   10                  15      


Lys Glu Glu Thr Val Met Leu Tyr His Glu Leu Glu Val Ala Ala Ser 
            20                  25                  30          


Ala Asp Glu Val Trp Ser Val Glu Gly Ser Pro Glu Leu Gly Leu His 
        35                  40                  45              


Leu Pro Asp Leu Leu Pro Ala Gly Ile Phe Ala Lys Phe Glu Ile Thr 
    50                  55                  60                  


Gly Asp Gly Gly Glu Gly Ser Ile Leu Asp Met Thr Phe Pro Pro Gly 
65                  70                  75                  80  


Gln Phe Pro His His Tyr Arg Glu Lys Phe Val Phe Phe Asp His Lys 
                85                  90                  95      


Asn Arg Tyr Lys Leu Val Glu Gln Ile Asp Gly Asp Phe Phe Asp Leu 
            100                 105                 110         


Gly Val Thr Tyr Tyr Met Asp Thr Ile Arg Val Val Ala Thr Gly Pro 
        115                 120                 125             


Asp Ser Cys Val Ile Lys Ser Thr Thr Glu Tyr His Val Lys Pro Glu 
    130                 135                 140                 


Phe Ala Lys Ile Val Lys Pro Leu Ile Asp Thr Val Pro Leu Ala Ile 
145                 150                 155                 160 


Met Ser Glu Ala Ile Ala Lys Val Val Leu Glu Asn Lys His Lys Ser 
                165                 170                 175     


Ser Glu 
        


<210>  15
<211>  537
<212>  DNA
<213>  Coptis japonica

<400>  15
atggaaagat tgatcttcaa tggtagacct ttgttgcaca gagttaccaa agaagaaacc       60

gttatgttgt accacgaatt ggaagttgct gcttctgctg atgaagtttg gtctgttgaa      120

ggttctccag aattgggttt acatttgcca gatttgttgc cagctggtat ttttgccaag      180

ttcgaaatta ctggtgatgg tggtgaaggt tccattttgg atatgacttt tccaccaggt      240

caattcccac atcattacag agaaaagttc gtctttttcg accacaagaa cagatacaag      300

ttggtcgaac aaatcgatgg tgatttcttc gatttgggtg ttacttacta catggacacc      360

attagagttg ttgctactgg tccagattct tgcgttatta agtctactac tgaataccac      420

gtcaagccag aatttgctaa aatcgttaag ccattgatcg ataccgttcc attggctatt      480

atgtctgaag ctattgccaa ggttgtcttg gaaaacaaac acaagtcatc tgaatga         537


<210>  16
<211>  200
<212>  PRT
<213>  Coptis japonica

<400>  16

Met Arg Met Glu Val Val Leu Val Val Phe Leu Met Phe Ile Gly Thr 
1               5                   10                  15      


Ile Asn Cys Glu Arg Leu Ile Phe Asn Gly Arg Pro Leu Leu His Arg 
            20                  25                  30          


Val Thr Lys Glu Glu Thr Val Met Leu Tyr His Glu Leu Glu Val Ala 
        35                  40                  45              


Ala Ser Ala Asp Glu Val Trp Ser Val Glu Gly Ser Pro Glu Leu Gly 
    50                  55                  60                  


Leu His Leu Pro Asp Leu Leu Pro Ala Gly Ile Phe Ala Lys Phe Glu 
65                  70                  75                  80  


Ile Thr Gly Asp Gly Gly Glu Gly Ser Ile Leu Asp Met Thr Phe Pro 
                85                  90                  95      


Pro Gly Gln Phe Pro His His Tyr Arg Glu Lys Phe Val Phe Phe Asp 
            100                 105                 110         


His Lys Asn Arg Tyr Lys Leu Val Glu Gln Ile Asp Gly Asp Phe Phe 
        115                 120                 125             


Asp Leu Gly Val Thr Tyr Tyr Met Asp Thr Ile Arg Val Val Ala Thr 
    130                 135                 140                 


Gly Pro Asp Ser Cys Val Ile Lys Ser Thr Thr Glu Tyr His Val Lys 
145                 150                 155                 160 


Pro Glu Phe Ala Lys Ile Val Lys Pro Leu Ile Asp Thr Val Pro Leu 
                165                 170                 175     


Ala Ile Met Ser Glu Ala Ile Ala Lys Val Val Leu Glu Asn Lys His 
            180                 185                 190         


Lys Ser Ser Glu His Asp Glu Leu 
        195                 200 


<210>  17
<211>  603
<212>  DNA
<213>  Coptis japonica

<400>  17
atgagaatgg aagtcgtctt ggtcgttttc ttgatgttca ttggtactat caactgcgaa       60

agattgatct tcaatggtag acctttgttg cacagagtta ccaaagaaga aaccgttatg      120

ttgtaccacg aattggaagt tgctgcttct gctgatgaag tttggtctgt tgaaggttct      180

ccagaattgg gtttacattt gccagatttg ttgccagctg gtatttttgc caagttcgaa      240

attactggtg atggtggtga aggttccatt ttggatatga cttttccacc aggtcaattc      300

ccacatcatt acagagaaaa gttcgtcttt ttcgaccaca agaacagata caagttggtc      360

gaacaaatcg atggtgattt cttcgatttg ggtgttactt actacatgga caccattaga      420

gttgttgcta ctggtccaga ttcttgcgtt attaagtcta ctactgaata ccacgtcaag      480

ccagaatttg ctaaaatcgt taagccattg atcgataccg ttccattggc tattatgtct      540

gaagctattg ccaaggttgt cttggaaaac aaacacaagt catctgaaca tgatgaattg      600

tga                                                                    603


<210>  18
<211>  196
<212>  PRT
<213>  Coptis japonica

<400>  18

Met Arg Met Glu Val Val Leu Val Val Phe Leu Met Phe Ile Gly Thr 
1               5                   10                  15      


Ile Asn Cys Glu Arg Leu Ile Phe Asn Gly Arg Pro Leu Leu His Arg 
            20                  25                  30          


Val Thr Lys Glu Glu Thr Val Met Leu Tyr His Glu Leu Glu Val Ala 
        35                  40                  45              


Ala Ser Ala Asp Glu Val Trp Ser Val Glu Gly Ser Pro Glu Leu Gly 
    50                  55                  60                  


Leu His Leu Pro Asp Leu Leu Pro Ala Gly Ile Phe Ala Lys Phe Glu 
65                  70                  75                  80  


Ile Thr Gly Asp Gly Gly Glu Gly Ser Ile Leu Asp Met Thr Phe Pro 
                85                  90                  95      


Pro Gly Gln Phe Pro His His Tyr Arg Glu Lys Phe Val Phe Phe Asp 
            100                 105                 110         


His Lys Asn Arg Tyr Lys Leu Val Glu Gln Ile Asp Gly Asp Phe Phe 
        115                 120                 125             


Asp Leu Gly Val Thr Tyr Tyr Met Asp Thr Ile Arg Val Val Ala Thr 
    130                 135                 140                 


Gly Pro Asp Ser Cys Val Ile Lys Ser Thr Thr Glu Tyr His Val Lys 
145                 150                 155                 160 


Pro Glu Phe Ala Lys Ile Val Lys Pro Leu Ile Asp Thr Val Pro Leu 
                165                 170                 175     


Ala Asp Met Ser Glu Ala Ile Ala Lys Val Val Leu Glu Asn Lys His 
            180                 185                 190         


Lys Ser Ser Glu 
        195     


<210>  19
<211>  591
<212>  DNA
<213>  Coptis japonica

<400>  19
atgagaatgg aagtcgtctt ggtcgttttc ttgatgttca ttggtactat caactgcgaa       60

agattgatct tcaatggtag acctttgttg cacagagtta ccaaagaaga aaccgttatg      120

ttgtaccacg aattggaagt tgctgcttct gctgatgaag tttggtctgt tgaaggttct      180

ccagaattgg gtttacattt gccagatttg ttgccagctg gtatttttgc caagttcgaa      240

attactggtg atggtggtga aggttccatt ttggatatga cttttccacc aggtcaattc      300

ccacatcatt acagagaaaa gttcgtcttt ttcgaccaca agaacagata caagttggtc      360

gaacaaatcg atggtgattt cttcgatttg ggtgttactt actacatgga caccattaga      420

gttgttgcta ctggtccaga ttcttgcgtt attaagtcta ctactgaata ccacgtcaag      480

ccagaatttg ctaaaatcgt taagccattg atcgataccg ttccattggc tgatatgtct      540

gaagctattg ccaaggttgt cttggaaaac aaacacaagt catctgaatg a               591


<210>  20
<211>  200
<212>  PRT
<213>  Coptis japonica

<400>  20

Met Arg Met Glu Val Val Leu Val Val Phe Leu Met Phe Ile Gly Thr 
1               5                   10                  15      


Ile Asn Cys Glu Arg Leu Ile Phe Asn Gly Arg Pro Leu Leu His Arg 
            20                  25                  30          


Val Thr Lys Glu Glu Thr Val Met Leu Tyr His Glu Leu Glu Val Ala 
        35                  40                  45              


Ala Ser Ala Asp Glu Val Trp Ser Val Glu Gly Ser Pro Glu Leu Gly 
    50                  55                  60                  


Leu His Leu Pro Asp Leu Leu Pro Ala Gly Ile Phe Ala Lys Phe Glu 
65                  70                  75                  80  


Ile Thr Gly Asp Gly Gly Glu Gly Ser Ile Leu Asp Met Thr Phe Pro 
                85                  90                  95      


Pro Gly Gln Phe Pro His His Tyr Arg Glu Lys Phe Val Phe Phe Asp 
            100                 105                 110         


His Lys Asn Arg Tyr Lys Leu Val Glu Gln Ile Asp Gly Asp Phe Phe 
        115                 120                 125             


Asp Leu Gly Val Thr Tyr Tyr Met Asp Thr Ile Arg Val Val Ala Thr 
    130                 135                 140                 


Gly Pro Asp Ser Cys Val Ile Lys Ser Thr Thr Glu Tyr His Val Lys 
145                 150                 155                 160 


Pro Glu Phe Ala Lys Ile Val Lys Pro Leu Ile Asp Thr Val Pro Leu 
                165                 170                 175     


Ala Asp Met Ser Glu Ala Ile Ala Lys Val Val Leu Glu Asn Lys His 
            180                 185                 190         


Lys Ser Ser Glu His Asp Glu Leu 
        195                 200 


<210>  21
<211>  603
<212>  DNA
<213>  Coptis japonica

<400>  21
atgagaatgg aagtcgtctt ggtcgttttc ttgatgttca ttggtactat caactgcgaa       60

agattgatct tcaatggtag acctttgttg cacagagtta ccaaagaaga aaccgttatg      120

ttgtaccacg aattggaagt tgctgcttct gctgatgaag tttggtctgt tgaaggttct      180

ccagaattgg gtttacattt gccagatttg ttgccagctg gtatttttgc caagttcgaa      240

attactggtg atggtggtga aggttccatt ttggatatga cttttccacc aggtcaattc      300

ccacatcatt acagagaaaa gttcgtcttt ttcgaccaca agaacagata caagttggtc      360

gaacaaatcg atggtgattt cttcgatttg ggtgttactt actacatgga caccattaga      420

gttgttgcta ctggtccaga ttcttgcgtt attaagtcta ctactgaata ccacgtcaag      480

ccagaatttg ctaaaatcgt taagccattg atcgataccg ttccattggc tgatatgtct      540

gaagctattg ccaaggttgt cttggaaaac aaacacaagt catctgaaca tgatgaattg      600

tga                                                                    603


