                         SEQUENCE LISTING

<110>  ARC BIO, LLC
 
<120>  ARCB-005/03WO 329757-2055

<130>  CREATION AND USE OF GUIDE NUCLEIC ACIDS

<140>  PCT/US2018/036563
<141>  2018-06-07

<150>  US 62/548,036
<151>  2017-08-21

<150>  US 62/516,619
<151>  2017-06-07

<160>  57    

<170>  PatentIn version 3.5

<210>  1
<211>  83
<212>  DNA
<213>  Unknown

<220>  
<223>  gNA stem loop sequence

<400>  1
gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt ttt                                               83


<210>  2
<211>  83
<212>  DNA
<213>  Unknown

<220>  
<223>  gNA stem loop sequence

<400>  2
aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc cttattttaa       60

cttgctattt ctagctctaa aac                                               83


<210>  3
<211>  83
<212>  RNA
<213>  Unknown

<220>  
<223>  gNA stem loop sequence

<400>  3
guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc cguuaucaac uugaaaaagu       60

ggcaccgagu cggugcuuuu uuu                                               83


<210>  4
<211>  94
<212>  DNA
<213>  Unknown

<220>  
<223>  gNA stem loop sequence

<400>  4
gttttagagc tatgctggaa acagcatagc aagttaaaat aaggctagtc cgttatcaac       60

ttgaaaaagt ggcaccgagt cggtgctttt tttc                                   94


<210>  5
<211>  94
<212>  DNA
<213>  Unknown

<220>  
<223>  gNA stem loop sequence

<400>  5
gaaaaaaagc accgactcgg tgccactttt tcaagttgat aacggactag ccttatttta       60

acttgctatg ctgtttccag catagctcta aaac                                   94


<210>  6
<211>  94
<212>  RNA
<213>  Unknown

<220>  
<223>  gNA stem loop sequence

<400>  6
guuuuagagc uaugcuggaa acagcauagc aaguuaaaau aaggcuaguc cguuaucaac       60

uugaaaaagu ggcaccgagu cggugcuuuu uuuc                                   94


<210>  7
<211>  19
<212>  RNA
<213>  Unknown

<220>  
<223>  gNA stem loop sequence

<400>  7
aauuucuacu guuguagau                                                    19


<210>  8
<211>  19
<212>  DNA
<213>  Unknown

<220>  
<223>  gNA stem loop sequence

<400>  8
aatttctact gttgtagat                                                    19


<210>  9
<211>  22
<212>  DNA
<213>  Unknown

<220>  
<223>  CRISPR RNA (crRNA)

<400>  9
gttttagagc tatgctgttt tg                                                22


<210>  10
<211>  86
<212>  DNA
<213>  Unknown

<220>  
<223>  trans-activating crRNA (tracrRNA)

<400>  10
ggaaccattc aaaacagcat agcaagttaa aataaggcta gtccgttatc aacttgaaaa       60

agtggcaccg agtcggtgct tttttt                                            86


<210>  11
<211>  22
<212>  DNA
<213>  Unknown

<220>  
<223>  gNA stem loop sequence

<400>  11
gttttagagc tatgctgttt tg                                                22


<210>  12
<211>  22
<212>  DNA
<213>  Unknown

<220>  
<223>  gNA stem loop sequence

<400>  12
caaaacagca tagctctaaa ac                                                22


<210>  13
<211>  86
<212>  RNA
<213>  Unknown

<220>  
<223>  trans-activating crRNA (tracrRNA)

<400>  13
ggaaccauuc aaaacagcau agcaaguuaa aauaaggcua guccguuauc aacuugaaaa       60

aguggcaccg agucggugcu uuuuuu                                            86


<210>  14
<211>  29
<212>  DNA
<213>  Unknown

<220>  
<223>  T7 promoter variant

<400>  14
gcctcgagct aatacgactc actatagag                                         29


<210>  15
<211>  19
<212>  DNA
<213>  Unknown

<220>  
<223>  T7 promoter variant

<400>  15
taatacgact cactatagg                                                    19


<210>  16
<211>  20
<212>  DNA
<213>  Unknown

<220>  
<223>  T7 promoter variant

<400>  16
taatacgact cactataggg                                                   20


<210>  17
<211>  19
<212>  DNA
<213>  Unknown

<220>  
<223>  gNA stem loop sequence

<400>  17
atctacaaca gtagaaatt                                                    19


<210>  18
<211>  22
<212>  RNA
<213>  Unknown

<220>  
<223>  trans-activating crRNA (tracrRNA)

<400>  18
guuuuagagc uaugcuguuu ug                                                22


<210>  19
<211>  21
<212>  DNA
<213>  Unknown

<220>  
<223>  Streptococcus pyogenes 3 prime Adapter sequence with type IIS enzyme site

<400>  19
gggactcgga tccctatagt c                                                 21


<210>  20
<211>  46
<212>  DNA
<213>  Unknown

<220>  
<223>  Staphylococcus aureus 3 prime Adapter sequence with type IIS enzyme site

<400>  20
ttttagcggc cgcctgctgc tctacaaaga cgatgacgac aagcgt                      46


<210>  21
<211>  50
<212>  DNA
<213>  Unknown

<220>  
<223>  Neisseria meningitides 3 prime Adapter sequence with type IIS enzyme site

<400>  21
tcgcggccgc ttttattctg ctgctctaca aagacgatga cgacaagcgt                  50


<210>  22
<211>  50
<212>  DNA
<213>  Unknown

<220>  
<223>  Streptococcus thermophilus 3 prime Adapter sequence with type IIS enzyme site

<400>  22
ttgcggccgc ttttattctg ctgctctaca aagacgatga cgacaagcgt                  50


<210>  23
<211>  45
<212>  DNA
<213>  Unknown

<220>  
<223>  Treponema denticola 3 prime Adapter sequence with type IIS enzyme site

<400>  23
tttagcggcc gcctgctgct ctacaaagac gatgacgaca agcgt                       45


<210>  24
<211>  47
<212>  DNA
<213>  Unknown

<220>  
<223>  Streptococcus pyogenes Adapter oligo 1 MlyI-1

<400>  24
gggggactcg gatccctata gtgatacaaa gacgatgacg acaagcg                     47


<210>  25
<211>  40
<212>  DNA
<213>  Unknown

<220>  
<223>  Streptococcus pyogenes Adapter oligo 2 T7-7


<220>
<221>  misc_feature
<222>  (9)..(12)
<223>  joined by phosphorothioate backbone linkages

<400>  25
gcctcgagct aatacgactc actataggga tccaagtccc                             40


<210>  26
<211>  47
<212>  DNA
<213>  Unknown

<220>  
<223>  Staphylococcus aureus Adapter oligo 1


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is inosine

<400>  26
nttttagcgg ccgcctgctg ctctacaaag acgatgacga caagcgt                     47


<210>  27
<211>  45
<212>  DNA
<213>  Unknown

<220>  
<223>  Staphylococcus aureus Adapter oligo 2

<400>  27
gagatcagct tctgcattga tgcgagcagc aggcggccgc taaaa                       45


<210>  28
<211>  53
<212>  DNA
<213>  Unknown

<220>  
<223>  Neisseria meningitides Adapter oligo 1

<400>  28
atttcgcggc cgcttttatt ctgctgctct acaaagacga tgacgacaag cgt              53


<210>  29
<211>  49
<212>  DNA
<213>  Unknown

<220>  
<223>  Neisseria meningitides Adapter oligo 2

<400>  29
gagatcagct tctgcattga tgcgagcagc agaataaaag cggccgcga                   49


<210>  30
<211>  48
<212>  DNA
<213>  Unknown

<220>  
<223>  Streptococcus thermophilus Adapter oligo 1

<400>  30
gcggccgctt ttattctgct gctctacaaa gacgatgacg acaagcgt                    48


<210>  31
<211>  49
<212>  DNA
<213>  Unknown

<220>  
<223>  Streptococcus thermophilus Adapter oligo 2


<220>
<221>  misc_feature
<222>  (48)..(48)
<223>  n is inosine

<400>  31
gagatcagct tctgcattga tgcgagcagc agaataaaag cggccgcng                   49


<210>  32
<211>  39
<212>  DNA
<213>  Unknown

<220>  
<223>  MlyI-Ad1 oligonucleotide

<400>  32
gagatcagct tctgcattga tgccagcagc ccgagtcag                              39


<210>  33
<211>  42
<212>  DNA
<213>  Unknown

<220>  
<223>  MlyI-Ad2 oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Phosphate moiety attached at 5 prime end

<400>  33
ctgactcggg ctgctgtaca aagacgatga cgacaagcgt ta                          42


<210>  34
<211>  46
<212>  DNA
<213>  Unknown

<220>  
<223>  BsMm-Ad1 oligonucleotide

<400>  34
gagatcagct tctgcattga tgcggagccg cagtacacta tccaac                      46


<210>  35
<211>  46
<212>  DNA
<213>  Unknown

<220>  
<223>  BsMm-Ad2 oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Phosphate moiety attached at 5 prime end

<400>  35
gttggatagt gtactgcggc tcctacaaag acgatgacga caagcg                      46


<210>  36
<211>  31
<212>  DNA
<213>  Unknown

<220>  
<223>  T7-Ad1 and T7-Ad3 oligonucleotide


<220>
<221>  misc_feature
<222>  (30)..(31)
<223>  n is a, c, g, or t

<400>  36
gcctcgagct aatacgactc actatagagn n                                      31


<210>  37
<211>  20
<212>  DNA
<213>  Unknown

<220>  
<223>  T7-Ad2, T7-Ad4 and T7-2 oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Phosphate moiety attached at 5 prime end

<400>  37
ctctatagtg agtcgtatta                                                   20


<210>  38
<211>  79
<212>  DNA
<213>  Unknown

<220>  
<223>  gR-top oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Phosphate moiety attached at 5 prime end

<400>  38
ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc       60

accgagtcgg tgctttttt                                                    79


<210>  39
<211>  82
<212>  DNA
<213>  Unknown

<220>  
<223>  gR-bot oligonucleotide

<400>  39
aaaaaagcac cgactcggtg ccactttttc aagttgataa cggactagcc ttattttaac       60

ttgctatttc tagctctaaa ac                                                82


<210>  40
<211>  29
<212>  DNA
<213>  Unknown

<220>  
<223>  T7-1 oligonucleotide


<220>
<221>  misc_feature
<222>  (9)..(12)
<223>  joined by phosphorothioate backbone linkages

<400>  40
gcctcgagct aatacgactc actatagag                                         29


<210>  41
<211>  45
<212>  DNA
<213>  Unknown

<220>  
<223>  MlyI-1 oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Phosphate moiety attached at 5 prime end

<400>  41
gggactcgga tccctatagt gatacaaaga cgatgacgac aagcg                       45


<210>  42
<211>  22
<212>  DNA
<213>  Unknown

<220>  
<223>  MlyI-2 oligonucleotide

<400>  42
tcactatagg gatccgagtc cc                                                22


<210>  43
<211>  94
<212>  DNA
<213>  Unknown

<220>  
<223>  stlgR oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Phosphate moiety attached at 5 prime end

<400>  43
gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt tttggatccg atgc                                   94


<210>  44
<211>  89
<212>  DNA
<213>  Unknown

<220>  
<223>  stlgRev oligonucleotide

<400>  44
ggatccaaaa aaagcaccga ctcggtgcca ctttttcaag ttgataacgg actagcctta       60

ttttaacttg ctatttctag ctctaaaac                                         89


<210>  45
<211>  21
<212>  DNA
<213>  Unknown

<220>  
<223>  gRU oligonucleotide

<400>  45
aaaaaaagca ccgactcggt g                                                 21


<210>  46
<211>  29
<212>  DNA
<213>  Unknown

<220>  
<223>  T7-17 oligonucleotide


<220>
<221>  misc_feature
<222>  (9)..(12)
<223>  joined by phosphorothioate backbone linkages

<400>  46
gcctcgagct aatacgactc actataggg                                         29


<210>  47
<211>  23
<212>  DNA
<213>  Unknown

<220>  
<223>  Flag oligonucleotide

<400>  47
cgcttgtcgt catcgtcttt gta                                               23


<210>  48
<211>  20
<212>  DNA
<213>  Unknown

<220>  
<223>  gRU variant oligonucleotide

<400>  48
aaaaaagcac cgactcggtg                                                   20


<210>  49
<211>  64
<212>  DNA
<213>  Unknown

<220>  
<223>  BE Ad1 oligonucleotide used with BaeI/EcoP15I Adapter


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Phosphate moiety attached at 5 prime end

<400>  49
actgctgaca caagtatctt ttttttttgt ttaaactttt ttttttgata cacaagtcag       60

caga                                                                    64


<210>  50
<211>  64
<212>  DNA
<213>  Unknown

<220>  
<223>  Be Ad2 oligonucleotide used with BaeI/EcoP15I Adapter


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Phosphate moiety attached at 5 prime end

<400>  50
tctgctgact tgtgtatcaa aaaaaaaagt ttaaacaaaa aaaaaagata cttgtgtcag       60

cagt                                                                    64


<210>  51
<211>  29
<212>  DNA
<213>  Unknown

<220>  
<223>  T7-Ad3 oligonucleotide used with BaeI/EcoP15I Adapter

<400>  51
gcctcgagct aatacgactc actatagag                                         29


<210>  52
<211>  22
<212>  DNA
<213>  Unknown

<220>  
<223>  T7-Ad4 oligonucleotide used with BaeI/EcoP15I Adapter


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Phosphate moiety attached at 5 prime end

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  n is a, c, g, or t

<400>  52
nnctctatag tgagtcgtat ta                                                22


<210>  53
<211>  79
<212>  DNA
<213>  Unknown

<220>  
<223>  stlgR oligonucleotide used with BaeI/EcoP15I Adapter


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Adenylate moiety attached at 5 prime end

<400>  53
ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc       60

accgagtcgg tgctttttt                                                    79


<210>  54
<211>  69
<212>  DNA
<213>  Unknown

<220>  
<223>  circMF oligonucleotide used with FokI/MmeI Adapter


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Phosphate moiety attached at 5 prime end

<400>  54
ttggatcatc ctgtgaagct ttttcttttt cttttcactg cgcgaatctg cattcacagg       60

atgatccaa                                                               69


<210>  55
<211>  87
<212>  DNA
<213>  Unknown

<220>  
<223>  N4UstlgR oligonucleotide used with FokI/MmeI Adapter


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Phosphate moiety attached at 5 prime end

<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  n is a, c, g, t or u

<400>  55
nnnnguttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa       60

aagtggcacc gagtcggtgc ttttttt                                           87


<210>  56
<211>  61
<212>  DNA
<213>  Unknown

<220>  
<223>  MNA oligonucleotide used with FokI/MmeI Adapter

<400>  56
gagatcagct tctgcattga tgcggccgct tattttaact tgctatttct agctctaaaa       60

c                                                                       61


<210>  57
<211>  37
<212>  DNA
<213>  Unknown

<220>  
<223>  T7-RND8 oligonucleotide


<220>
<221>  misc_feature
<222>  (30)..(37)
<223>  n is a, c, g, or t

<400>  57
gcctcgagct aatacgactc actatagagn nnnnnnn                                37


