                         SEQUENCE LISTING

<110>  Zymergen Inc.
       Kelly, Peter
       Enyeart, Peter  
 
<120>  HTP Transposon Mutagenesis

<130>  ZYMR-014/01WO 327574-2060

<150>  US 62/515,965 
<151>  2017-06-06

<160>  20   

<170>  PatentIn version 3.5

<210>  1
<211>  97
<212>  DNA
<213>  Unknown

<220>
<223>  Expression promoter derived from Pcg0007_lib_39

<400>  1
tgccgtttct cgcgttgtgt gtggtactac gtggggacct aagcgtgtat tatggaaacg       60

tctgtatcgg ataagtagcg aggagtgttc gttaaaa                                97


<210>  2
<211>  97
<212>  DNA
<213>  Unknown

<220>
<223>  Expression promoter derived from Pcg0007

<400>  2
tgccgtttct cgcgttgtgt gtggtactac gtggggacct aagcgtgtaa gatggaaacg       60

tctgtatcgg ataagtagcg aggagtgttc gttaaaa                                97


<210>  3
<211>  93
<212>  DNA
<213>  Unknown

<220>
<223>  Expression promoter derived from Pcg1860

<400>  3
cttagctttg acctgcacaa atagttgcaa attgtcccac atacacataa agtagcttgc       60

gtatttaaaa ttatgaacct aaggggttta gca                                    93


<210>  4
<211>  98
<212>  DNA
<213>  Unknown

<220>
<223>  Expression promoter derived from Pcg0755

<400>  4
aataaattta taccacacag tctattgcaa tagaccaagc tgttcagtag ggtgcatggg       60

agaagaattt cctaataaaa actcttaagg acctccaa                               98


<210>  5
<211>  97
<212>  DNA
<213>  Unknown

<220>
<223>  Expression promoter derived from Pcg0007_265

<400>  5
tgccgtttct cgcgttgtgt gtggtactac gtggggacct aagcgtgtac gctggaaacg       60

tctgtatcgg ataagtagcg aggagtgttc gttaaaa                                97


<210>  6
<211>  86
<212>  DNA
<213>  Unknown

<220>
<223>  Expression promoter derived from Pcg3381

<400>  6
cgccggataa atgaattgat tattttaggc tcccagggat taagtctagg gtggaatgca       60

gaaatatttc ctacggaagg tccgtt                                            86


<210>  7
<211>  97
<212>  DNA
<213>  Unknown

<220>
<223>  Expression promoter derived from Pcg0007_119

<400>  7
tgccgtttct cgcgttgtgt gtggtactac gtggggacct aagcgtgttg catggaaacg       60

tctgtatcgg ataagtagcg aggagtgttc gttaaaa                                97


<210>  8
<211>  87
<212>  DNA
<213>  Unknown

<220>
<223>  Expression promoter derived from Pcg3121

<400>  8
gtggctaaaa cttttggaaa cttaagttac ctttaatcgg aaacttattg aattcgggtg       60

aggcaactgc aactctggac ttaaagc                                           87


<210>  9
<211>  25
<212>  DNA
<213>  Unknown

<220>
<223>  cg0001 Terminator

<400>  9
gacccatctt cggatgggtc ttttt                                             25


<210>  10
<211>  30
<212>  DNA
<213>  Unknown

<220>
<223>  cg0007 Terminator

<400>  10
cccgcccctg gaattctggg ggcgggtttt                                        30


<210>  11
<211>  24
<212>  DNA
<213>  Unknown

<220>
<223>  cg0371 Terminator

<400>  11
ccggtaactt ttgtaagttg ccgg                                              24


<210>  12
<211>  27
<212>  DNA
<213>  Unknown

<220>
<223>  cg0480 Terminator

<400>  12
cccctcagaa gcgattctga ggggttt                                           27


<210>  13
<211>  28
<212>  DNA
<213>  Unknown

<220>
<223>  cg0494 Terminator

<400>  13
gcaccgcctt tcggggcggt gctttttt                                          28


<210>  14
<211>  28
<212>  DNA
<213>  Unknown

<220>
<223>  cg0564 Terminator

<400>  14
ggccccatgc tttgcatggg gtcttttt                                          28


<210>  15
<211>  30
<212>  DNA
<213>  Unknown

<220>
<223>  cg0610 Terminator

<400>  15
gcacttacct taactggtag gtgctttttt                                        30


<210>  16
<211>  24
<212>  DNA
<213>  Unknown

<220>
<223>  cg0695 Terminator

<400>  16
acccggtcac cagaccgggt cttt                                              24


<210>  17
<211>  1048
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Loss of Function transposon

<400>  17
ctgtctctta tacacatctc cggaattgcc agctggggcg ccctctggta aggttgggaa       60

gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc      120

aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca      180

cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac      240

aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt      300

tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc      360

gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg      420

aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc      480

tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc      540

ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat      600

ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc      660

cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca      720

tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga      780

ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat      840

tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc      900

tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaatcgatag      960

ccgccccgca gggcgctccg caggccgctt ccggaccact ccggaagcgg ccgtgcggtc     1020

ggaggtacca gatgtgtata agagacag                                        1048


<210>  18
<211>  1352
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gain of function transposon

<400>  18
ctgtctctta tacacatctc cggaattgcc agctggggcg ccctctggta aggttgggaa       60

gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc      120

aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca      180

cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac      240

aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt      300

tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc      360

gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg      420

aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc      480

tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc      540

ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat      600

ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc      660

cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca      720

tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga      780

ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat      840

tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc      900

tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaatcgatag      960

ccgccccgca gggcgctccg caggccgctt ccggaccact ccggaagcgg ccgtgcggtc     1020

ggaggtaccg gtaccagccc gacccgagca cgcgccggca cgcctggtcg atgtcggacc     1080

ggagttcgag gtacgcggct tgcaggtcca ggaaggggac gtccatgcga gtgtccgttc     1140

gagtggcggc ttgcgcccga tgctagtcgc ggttgatcgg cgatcgcagg tgcacgcggt     1200

cgatcttgac ggctggcgag aggtgcgggg aggatctgac cgacgcggtc cacacgtggc     1260

accgcgatgc tgttgtgggc acaatcgtgc cggttggtag gatccccacc caacgcaccc     1320

caggaggtcc catagatgtg tataagagac ag                                   1352


<210>  19
<211>  3068
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gain of Function recyclable transposon

<400>  19
ctgtctctta tacacatctg gtaccagccc gacccgagca cgcgccggca cgcctggtcg       60

atgtcggacc ggagttcgag gtacgcggct tgcaggtcca ggaaggggac gtccatgcga      120

gtgtccgttc gagtggcggc ttgcgcccga tgctagtcgc ggttgatcgg cgatcgcagg      180

tgcacgcggt cgatcttgac ggctggcgag aggtgcgggg aggatctgac cgacgcggtc      240

cacacgtggc accgcgatgc tgttgtgggc acaatcgtgc cggttggtag gatccccacc      300

caacgcaccc caggaggtcc cataagaggt atatattacc ggaattgcca gctggggcgc      360

cctctggtaa ggttgggaag ccctgcaaag taaactggat ggctttcttg ccgccaagga      420

tctgatggcg caggggatca agatctgatc aagagacagg atgaggatcg tttcgcatga      480

ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct      540

atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc      600

aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg      660

acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg      720

acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc      780

tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc      840

ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg      900

agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc      960

atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg     1020

aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc     1080

gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag     1140

cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg     1200

tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg     1260

agttcttctg atgcgcggcc ggacccgcac acacccgctc cagacgccca cgcaaggaga     1320

cccatgaaca tcaagaagtt cgccaagcgg gcgaccgtcc tgaccttcac caccgccctg     1380

ctcgcgggcg gggccaccca ggccttcgcc aaggagaaca cccagaagcc ctacaaggag     1440

acgtacgggg tgtcgcacat cacccgccac gacatgctcc agatccccaa gcagcagcag     1500

agcgagaagt accaggtccc gcagttcgac cagtccacca tcaagaacat cgaatcggcc     1560

aagggcctcg acgtgtggga ctcctggccc ctgcagaacg ccgacggcac cgtggccgag     1620

tacaacgggt accacgtggt gttcgccctg gcgggctccc ccaaggacgc cgacgacacc     1680

tcgatctaca tgttctacca gaaggtcggc gacaacagca tcgactcctg gaagaacgcg     1740

ggccgcgtct tcaaggacag cgacaagttc gacgcgaacg acgagatcct gaaggagcag     1800

acccaggagt ggtccggctc cgccaccttc acgtccgacg gcaagatccg gctcttctac     1860

acggacttct ccggcacgca ctacgggaag cagagcctca ccacggcgca ggtcaacgtg     1920

tcgaagtccg acgacaccct caagatcaac ggcgtggagg accacaagac gatcttcgac     1980

ggcgacggca agacctacca gaacgtgcag cagttcatcg acgagggcaa ctacacgtcg     2040

ggcgacaacc acacgctgcg cgacccccac tacgtggagg acaaggggca caagtacctg     2100

gtcttcgagg ccaacaccgg caccgacaac ggctaccagg gcgaggaatc cctgttcaac     2160

aaggcgtact acggcggcag cacgaacttc ttccgcaagg agagccagaa gctccagcag     2220

tcggccaaga agcgggacgc cgagctcgcc aacggcgcgc tgggcatggt ggagctgaac     2280

gacgactaca cgctgaagaa ggtcatgaag ccgctcatca cctccaacac cgtgacggac     2340

gagatcgagc gggcgaacgt cttcaagatg aacggcaagt ggtacctgtt caccgactcc     2400

cgcggctcca agatgaccat cgacggcatc aactcgaacg acatctacat gctgggttac     2460

gtctccaaca gcctgaccgg gccgtacaag ccgctcaaca agaccggcct ggtgctccag     2520

atgggcctgg acccgaacga cgtcaccttc acctactccc acttcgcggt gccccaggcg     2580

aagggcaaca acgtggtcat cacctcgtac atgacgaacc ggggcttctt cgaggacaag     2640

aaggccacct tcgccccctc cttcctgatg aacatcaagg gcaagaagac ctccgtggtg     2700

aagaacagca tcctggagca gggccagctc accgtcaaca actgaggtac cagcccgacc     2760

cgagcacgcg ccggcacgcc tggtcgatgt cggaccggag ttcgaggtac gcggcttgca     2820

ggtccaggaa ggggacgtcc atgcgagtgt ccgttcgagt ggcggcttgc gcccgatgct     2880

agtcgcggtt gatcggcgat cgcaggtgca cgcggtcgat cttgacggct ggcgagaggt     2940

gcggggagga tctgaccgac gcggtccaca cgtggcaccg cgatgctgtt gtgggcacaa     3000

tcgtgccggt tggtaggatc cccacccaac gcaccccagg aggtcccata gatgtgtata     3060

agagacag                                                              3068


<210>  20
<211>  1716
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gain of function - solubility tag transposon

<400>  20
ctgtctctta tacacatctc cggaattgcc agctggggcg ccctctggta aggttgggaa       60

gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc      120

aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca      180

cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac      240

aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt      300

tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc      360

gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg      420

aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc      480

tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc      540

ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat      600

ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc      660

cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca      720

tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga      780

ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat      840

tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc      900

tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaatcgatag      960

ccgccccgca gggcgctccg caggccgctt ccggaccact ccggaagcgg ccgtgcggtc     1020

ggaggtacca tgtcccctat actaggttat tggaaaatta agggccttgt gcaacccact     1080

cgacttcttt tggaatatct tgaagaaaaa tatgaagagc atttgtatga gcgcgatgaa     1140

ggtgataaat ggcgaaacaa aaagtttgaa ttgggtttgg agtttcccaa tcttccttat     1200

tatattgatg gtgatgttaa attaacacag tctatggcca tcatacgtta tatagctgac     1260

aagcacaaca tgttgggtgg ttgtccaaaa gagcgtgcag agatttcaat gcttgaagga     1320

gcggttttgg atattagata cggtgtttcg agaattgcat atagtaaaga ctttgaaact     1380

ctcaaagttg attttcttag caagctacct gaaatgctga aaatgttcga agatcgttta     1440

tgtcataaaa catatttaaa tggtgatcat gtaacccatc ctgacttcat gttgtatgac     1500

gctcttgatg ttgttttata catggaccca atgtgcctgg atgcgttccc aaaattagtt     1560

tgttttaaaa aacgtattga agctatccca caaattgata agtacttgaa atccagcaag     1620

tatatagcat ggcctttgca gggctggcaa gccacgtttg gtggtggcga ccatcctcca     1680

aaatcggatc tggttccaga tgtgtataag agacag                               1716




