                         SEQUENCE LISTING

<110>  The Regents of the University of California
       Zettlitz, Kirstin
       Wu, Anna
       Reiter, Robert
 
<120>  HUMANIZED ANTI-N-CADHERIN ANTIBODIES AND USES THEREOF

<130>  206030-0103-00-WO.607640

<150>  US 62/515,617
<151>  2017-06-06

<160>  42    

<170>  PatentIn version 3.5

<210>  1
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, hu1H7_VH v1

<400>  1

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Phe Thr Phe Thr Asp Tyr 
            20                  25                  30          


Leu Ile Gln Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Trp Ile Tyr Pro Gly Ser Gly Ser Ile Lys Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Met Thr Ala Asp Thr Ser Ile Asn Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Arg Gly Asp Trp Gly Gly Phe Phe Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  2
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, hu1H7_VH v2

<400>  2

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Phe Thr Phe Thr Asp Tyr 
            20                  25                  30          


Leu Ile Gln Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Trp Phe Tyr Pro Gly Ser Gly Ser Ile Lys Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Asp Arg Ala Thr Leu Thr Ala Asp Lys Ser Ile Asn Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Arg Gly Asp Trp Gly Gly Phe Phe Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  3
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 1H7_VH

<400>  3

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Phe Thr Phe Thr Asp Tyr 
            20                  25                  30          


Leu Ile Gln Trp Val Lys Gln Arg Ser Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Trp Phe Tyr Pro Gly Ser Gly Ser Ile Lys Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Asp Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Asn Thr Val Tyr 
65                  70                  75                  80  


Met Glu Ile Ser Arg Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Arg Gly Asp Trp Gly Gly Phe Phe Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Thr Leu Thr Val Ser Ser 
        115                 


<210>  4
<211>  98
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, IGHV1-2*02F

<400>  4

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Gly Tyr 
            20                  25                  30          


Tyr Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Pro Asn Ser Gly Gly Thr Asn Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Met Thr Arg Asp Arg Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg 
        


<210>  5
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, Hu1H7	Heavy Chain CDR1

<400>  5

Gly Phe Thr Phe Thr Asp Tyr Leu Ile Gln 
1               5                   10  


<210>  6
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, Hu1H7	Heavy Chain CDR2

<400>  6

Trp Ile Gly Trp Ile Tyr Pro Gly Ser Gly Ser Ile Lys Tyr Asn Glu 
1               5                   10                  15      


Lys Phe Gln Gly 
            20  


<210>  7
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, Hu1H7	Heavy Chain CDR3

<400>  7

Ala Arg Arg Gly Asp Trp Gly Gly Phe Phe Asp Tyr 
1               5                   10          


<210>  8
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, hu1H7_VL v1

<400>  8

Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Thr Ala Ser Ser Ser Val Ser Ser Ser 
            20                  25                  30          


Tyr Leu His Trp Tyr Gln Gln Lys Pro Gly Leu Ala Pro Arg Leu Trp 
        35                  40                  45              


Ile Phe Ser Thr Ser Asn Leu Ala Ser Gly Ile Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu 
65                  70                  75                  80  


Pro Glu Asp Phe Ala Val Tyr Tyr Cys His Gln Tyr His Arg Ser Leu 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  9
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, hu1H7_VL v2

<400>  9

Asp Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Thr Ala Ser Ser Ser Val Ser Ser Ser 
            20                  25                  30          


Tyr Leu His Trp Tyr Gln Gln Lys Pro Gly Ser Ala Pro Arg Leu Trp 
        35                  40                  45              


Ile Phe Ser Thr Ser Asn Leu Ala Ser Gly Ile Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Gly Ser Gly Ser Thr Asp Tyr Thr Leu Thr Ile Ser Arg Leu Glu 
65                  70                  75                  80  


Pro Glu Asp Phe Ala Val Tyr Tyr Cys His Gln Tyr His Arg Ser Leu 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  10
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 1H7_VL

<400>  10

Gln Ile Val Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Leu Gly 
1               5                   10                  15      


Glu Arg Val Thr Met Thr Cys Thr Ala Ser Ser Ser Val Ser Ser Ser 
            20                  25                  30          


Tyr Leu His Trp Tyr Gln Gln Lys Pro Gly Ser Ser Pro Lys Leu Trp 
        35                  40                  45              


Ile Phe Ser Thr Ser Asn Leu Ala Ser Gly Val Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Asn Ser Met Glu 
65                  70                  75                  80  


Ala Glu Asp Ala Ala Thr Tyr Tyr Cys His Gln Tyr His Arg Ser Leu 
                85                  90                  95      


Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105         


<210>  11
<211>  96
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, IGKV3D-20 (A11)

<400>  11

Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Gly Ala Ser Gln Ser Val Ser Ser Ser 
            20                  25                  30          


Tyr Leu Ala Trp Tyr Ala Ala Lys Pro Gly Leu Ala Pro Arg Leu Leu 
        35                  40                  45              


Ile Tyr Asp Ala Ser Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu 
65                  70                  75                  80  


Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Ser Ser Pro 
                85                  90                  95      


<210>  12
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, Hu1H7	Light Chain CDR1

<400>  12

Thr Ala Ser Ser Ser Val Ser Ser Ser Tyr Leu His Trp Tyr 
1               5                   10                  


<210>  13
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, Hu1H7	Light Chain CDR2

<400>  13

Leu Trp Ile Phe Ser Thr Ser Asn Leu Ala Ser 
1               5                   10      


<210>  14
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, Hu1H7	Light Chain CDR3

<400>  14

His Gln Tyr His Arg Ser Leu Thr 
1               5               


<210>  15
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, hu2A9 VH

<400>  15

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Trp Met Gln Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Ala Ile Tyr Pro Gly Asp Gly Glu Thr Thr Tyr Thr Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Met Thr Ala Asp Thr Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Gly Asp Gly Tyr Trp Ala Met Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  16
<211>  98
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, IGHV1-2*02F

<400>  16

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Tyr Gly Tyr 
            20                  25                  30          


Tyr Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Pro Asn Ser Gly Gly Thr Asn Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Met Thr Arg Asp Thr Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg 
        


<210>  17
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 2A9_VH

<400>  17

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Trp Met Gln Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Ala Ile Tyr Pro Gly Asp Gly Glu Thr Thr Tyr Thr Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Ala Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Gly Asp Gly Tyr Trp Ala Met Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Ser Val Thr Val Ser Ser 
        115             


<210>  18
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, Hu2A9	Heavy Chain CDR1

<400>  18

Gly Tyr Thr Phe Thr Ser Tyr Trp Met Gln 
1               5                   10  


<210>  19
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, Hu2A9	Heavy Chain CDR2

<400>  19

Trp Ile Gly Ala Ile Tyr Pro Gly Asp Gly Glu Thr Thr Tyr Thr Gln 
1               5                   10                  15      


Lys Phe Lys Gly 
            20  


<210>  20
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, Hu2A9	Heavy Chain CDR3

<400>  20

Ala Lys Gly Asp Gly Tyr Trp Ala Met Asp Tyr 
1               5                   10      


<210>  21
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, hu2A9 VL

<400>  21

Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Leu Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Tyr Gln Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Arg Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Ser Gln Ser 
                85                  90                  95      


Thr His Val Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105                 110         


<210>  22
<211>  100
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, IGKV2-30*02

<400>  22

Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Leu Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asp Gly Asn Thr Tyr Leu Asn Trp Phe Gln Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Arg Arg Leu Ile Tyr Lys Val Ser Asn Arg Asp Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gln Gly 
                85                  90                  95      


Thr His Trp Pro 
            100 


<210>  23
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 2A9_VL

<400>  23

Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Ser 
                85                  90                  95      


Thr His Val Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105                 110         


<210>  24
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, Hu2A9	Light Chain CDR1

<400>  24

Arg Ser Ser Gln Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His 
1               5                   10                  15      


Trp Tyr 
        


<210>  25
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, Hu2A9	Light Chain CDR2

<400>  25

Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser 
1               5                   10      


<210>  26
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, Hu2A9	Light Chain CDR3

<400>  26

Ser Gln Ser Thr His Val Pro Leu Thr 
1               5                   


<210>  27
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  27
ggcttcacct tcaccgacta cctgatccag                                        30


<210>  28
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  28
tggatcggct ggatctaccc tggcagcggc agcatcaagt acaacgagaa gttccagggc       60


<210>  29
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  29
gccagaagag gcgactgggg cggcttcttc gattac                                 36


<210>  30
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  30
accgctagca gcagcgtgtc cagcagctac ctgcactggt at                          42


<210>  31
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  31
ctgtggatct tcagcaccag caatctggcc tcc                                    33


<210>  32
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  32
caccagtacc acagaagcct gacc                                              24


<210>  33
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  33
ggctacacct tcaccagcta ctggatgcag                                        30


<210>  34
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  34
tggatcggcg ccatctatcc tggcgacggc gagacaacct acacccagaa attcaagggc       60


<210>  35
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  35
gccaagggcg acggctactg ggctatggat tat                                    33


<210>  36
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  36
cggagcagcc agagcctggt gcacagcaac ggcaacacct acctgcactg gtat             54


<210>  37
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  37
ctgctgatct acaaggtgtc caacagattc agc                                    33


<210>  38
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  38
tcccagagca cccacgtgcc cctgacc                                           27


<210>  39
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, JH4

<400>  39

Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
1               5                   10                  15  


<210>  40
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, IGKJ4

<400>  40

Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
1               5                   10          


<210>  41
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, JH4

<400>  41

Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
1               5                   10                  15  


<210>  42
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, IGKJ4

<400>  42

Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
1               5                   10          


