                         SEQUENCE LISTING

<110>  VIB VZW
       KATHOLIEKE UNIVERSITEIT LEUVEN, K.U.LEUVEN R&D
 
<120>  GLUTAMINE DEHYDROGENASE INHIBITORS FOR USE IN MUSCLE REGENERATION

<130>  MM/GLUD1/591

<150>  EP 17175210.8
<151>  2017-06-09

<150>  EP 18156309.9
<151>  2018-02-12

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  3051
<212>  DNA
<213>  Homo sapiens

<400>  1
gggcaacccg cgcgggaccc ttcctcccta gtcgcgggga gtctgagaaa gcgcgcctgt       60

ttcgcgacca tcacgcacct cccctccgct tgtggccatg taccgctacc tgggcgaagc      120

gctgttgctg tcccgggccg ggcccgctgc cctgggctcg gcgtccgccg actcggccgc      180

gttgctgggc tgggcccggg gacagcccgc cgccgccccg cagccggggc tggcattggc      240

cgcccggcgc cactacagcg aggcggtggc cgaccgcgag gacgacccca acttcttcaa      300

gatggtggag ggcttcttcg atcgcggcgc cagcatcgtg gaggacaagc tggtggagga      360

cctgaggacc cgggagagcg aggagcagaa gcggaaccgg gtgcgcggca tcctgcggat      420

catcaagccc tgcaaccatg tgctgagtct ctccttcccc atccggcgcg acgacggctc      480

ctgggaggtc atcgaaggct accgggccca gcacagccag caccgcacgc cctgcaaggg      540

aggtatccgt tacagcactg atgtgagtgt agatgaagta aaagctttgg cttctctgat      600

gacatacaag tgtgcagtgg ttgatgtgcc gtttgggggt gctaaagctg gtgttaagat      660

caatcccaag aactatactg ataatgaatt ggaaaagatc acaaggaggt tcaccatgga      720

gctagcaaaa aagggcttta ttggtcctgg cattgatgtg cctgctccag acatgagcac      780

aggtgagcgg gagatgtcct ggatcgctga tacctatgcc agcaccatag ggcactatga      840

tattaatgca cacgcctgtg ttactggtaa acccatcagc caagggggaa tccatggacg      900

catctctgct actggccgtg gtgtcttcca tgggattgaa aatttcatca atgaagcttc      960

ttacatgagc attttaggaa tgacaccagg gtttggagat aaaacatttg ttgttcaggg     1020

atttggtaat gtgggcctac actctatgag atatttacat cgttttggtg ctaaatgtat     1080

tgctgttggt gagtctgatg ggagtatatg gaatccagat ggtattgacc caaaggaact     1140

ggaagacttc aaattgcaac atgggtccat tctgggcttc cccaaggcaa agccctatga     1200

aggaagcatc ttggaggccg actgtgacat actgatccca gctgccagtg agaagcagtt     1260

gaccaaatcc aacgcaccca gagtcaaagc caagatcatt gctgaaggtg ccaatgggcc     1320

aacaactcca gaagctgaca agatcttcct ggagagaaac attatggtta ttccagatct     1380

ctacttgaat gctggaggag tgacagtatc ttactttgag tggctgaaga atctaaatca     1440

tgtcagctat ggccgtttga ccttcaaata tgaaagggat tctaactacc acttgctcat     1500

gtctgttcaa gagagtttag aaagaaaatt tggaaagcat ggtggaacta ttcccattgt     1560

acccacggca gagttccaag acaggatatc gggtgcatct gagaaagaca tcgtgcactc     1620

tggcttggca tacacaatgg agcgttctgc caggcaaatt atgcgcacag ccatgaagta     1680

taacctggga ttggacctga gaacagctgc ctatgttaat gccattgaga aagtcttcaa     1740

agtgtacaat gaagctggtg tgaccttcac atagatggat catggctgac ttcctcacta     1800

tcctcttcac atgtaacttc tgcagaccta tcacaagttt acatgtaacc acagaaatcc     1860

ctttctctcc tgactcatta ataatggata ccattctcaa caagtcaatc caagtcagcc     1920

cgttaaggag aaagaaatta aggttagcgg atcatgtaca agctgagtgt gaaagtagaa     1980

atcacctaca ccagagagcc attttggtat tttgccttta aataaaaagc ctcctttatc     2040

tggctgtgca gccttgctct gtggcttttc ccaacacaat cagtgctagt gctggggagg     2100

aacagtcaag agcagtcagt tgcttgctta ttttttctgg atgagtctgg gacacactgt     2160

aactttaaca catttaagaa gtaggtgtgt ggccttttca gaaggtggca tggtcctcaa     2220

gtgagttctt agtattttat atcagcaaaa taattcaatt ttgcaggttg caaacaaata     2280

taaaacctgt ttctgtttat gaatattatt cttttagaat agaataagta catgctgctg     2340

taataaaatt gcctttaatc acttaacaag cctaaccttg actcaaacag tgaatgccta     2400

tagaaataat aaatgaaaaa aactagtatt tttatatcat aaaacaatgt catttatagc     2460

ttatcattca tgtattgtcc agcagacatt aaaagccctg tggataatta agttatcttc     2520

atacctgcaa aatggtggag gctattttca ttaaaactgt cagaatttgc ttaccataat     2580

tatgatacag tccaaagaat gcagtcactt tttatcatgt taactaattg ttctcttttg     2640

aagatctatg gttgactaat taaacaataa ttcaagtaga gtgtcccaga aaaaaaccac     2700

ttgggctccc tgtttggagt ctggctggct ctgagcattg ccaatggccc ctactcacct     2760

gactttgtat cctctccttt tagaggcttt gcattctgca cccagcttca ctaacagtgg     2820

gctgaaaaca tccttgggtt gagtgtttca tttgggagtt atttggccag ggccttttga     2880

acagtagtgt ccccatgaag tgctagataa tatatgtgta agagtcagct tttttttttt     2940

tttttaactc taacaccctt cagaaatttc taactacttt gtaactgcat ggcttaacct     3000

ggtgataaaa gcagttatta aaagtctacg ttttccaaaa aaaaaaaaaa a              3051


<210>  2
<211>  558
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Tyr Arg Tyr Leu Gly Glu Ala Leu Leu Leu Ser Arg Ala Gly Pro 
1               5                   10                  15      


Ala Ala Leu Gly Ser Ala Ser Ala Asp Ser Ala Ala Leu Leu Gly Trp 
            20                  25                  30          


Ala Arg Gly Gln Pro Ala Ala Ala Pro Gln Pro Gly Leu Ala Leu Ala 
        35                  40                  45              


Ala Arg Arg His Tyr Ser Glu Ala Val Ala Asp Arg Glu Asp Asp Pro 
    50                  55                  60                  


Asn Phe Phe Lys Met Val Glu Gly Phe Phe Asp Arg Gly Ala Ser Ile 
65                  70                  75                  80  


Val Glu Asp Lys Leu Val Glu Asp Leu Arg Thr Arg Glu Ser Glu Glu 
                85                  90                  95      


Gln Lys Arg Asn Arg Val Arg Gly Ile Leu Arg Ile Ile Lys Pro Cys 
            100                 105                 110         


Asn His Val Leu Ser Leu Ser Phe Pro Ile Arg Arg Asp Asp Gly Ser 
        115                 120                 125             


Trp Glu Val Ile Glu Gly Tyr Arg Ala Gln His Ser Gln His Arg Thr 
    130                 135                 140                 


Pro Cys Lys Gly Gly Ile Arg Tyr Ser Thr Asp Val Ser Val Asp Glu 
145                 150                 155                 160 


Val Lys Ala Leu Ala Ser Leu Met Thr Tyr Lys Cys Ala Val Val Asp 
                165                 170                 175     


Val Pro Phe Gly Gly Ala Lys Ala Gly Val Lys Ile Asn Pro Lys Asn 
            180                 185                 190         


Tyr Thr Asp Asn Glu Leu Glu Lys Ile Thr Arg Arg Phe Thr Met Glu 
        195                 200                 205             


Leu Ala Lys Lys Gly Phe Ile Gly Pro Gly Ile Asp Val Pro Ala Pro 
    210                 215                 220                 


Asp Met Ser Thr Gly Glu Arg Glu Met Ser Trp Ile Ala Asp Thr Tyr 
225                 230                 235                 240 


Ala Ser Thr Ile Gly His Tyr Asp Ile Asn Ala His Ala Cys Val Thr 
                245                 250                 255     


Gly Lys Pro Ile Ser Gln Gly Gly Ile His Gly Arg Ile Ser Ala Thr 
            260                 265                 270         


Gly Arg Gly Val Phe His Gly Ile Glu Asn Phe Ile Asn Glu Ala Ser 
        275                 280                 285             


Tyr Met Ser Ile Leu Gly Met Thr Pro Gly Phe Gly Asp Lys Thr Phe 
    290                 295                 300                 


Val Val Gln Gly Phe Gly Asn Val Gly Leu His Ser Met Arg Tyr Leu 
305                 310                 315                 320 


His Arg Phe Gly Ala Lys Cys Ile Ala Val Gly Glu Ser Asp Gly Ser 
                325                 330                 335     


Ile Trp Asn Pro Asp Gly Ile Asp Pro Lys Glu Leu Glu Asp Phe Lys 
            340                 345                 350         


Leu Gln His Gly Ser Ile Leu Gly Phe Pro Lys Ala Lys Pro Tyr Glu 
        355                 360                 365             


Gly Ser Ile Leu Glu Ala Asp Cys Asp Ile Leu Ile Pro Ala Ala Ser 
    370                 375                 380                 


Glu Lys Gln Leu Thr Lys Ser Asn Ala Pro Arg Val Lys Ala Lys Ile 
385                 390                 395                 400 


Ile Ala Glu Gly Ala Asn Gly Pro Thr Thr Pro Glu Ala Asp Lys Ile 
                405                 410                 415     


Phe Leu Glu Arg Asn Ile Met Val Ile Pro Asp Leu Tyr Leu Asn Ala 
            420                 425                 430         


Gly Gly Val Thr Val Ser Tyr Phe Glu Trp Leu Lys Asn Leu Asn His 
        435                 440                 445             


Val Ser Tyr Gly Arg Leu Thr Phe Lys Tyr Glu Arg Asp Ser Asn Tyr 
    450                 455                 460                 


His Leu Leu Met Ser Val Gln Glu Ser Leu Glu Arg Lys Phe Gly Lys 
465                 470                 475                 480 


His Gly Gly Thr Ile Pro Ile Val Pro Thr Ala Glu Phe Gln Asp Arg 
                485                 490                 495     


Ile Ser Gly Ala Ser Glu Lys Asp Ile Val His Ser Gly Leu Ala Tyr 
            500                 505                 510         


Thr Met Glu Arg Ser Ala Arg Gln Ile Met Arg Thr Ala Met Lys Tyr 
        515                 520                 525             


Asn Leu Gly Leu Asp Leu Arg Thr Ala Ala Tyr Val Asn Ala Ile Glu 
    530                 535                 540                 


Lys Val Phe Lys Val Tyr Asn Glu Ala Gly Val Thr Phe Thr 
545                 550                 555             


<210>  3
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  3
aatccctatc gattcctgtg                                                   20


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  4
gaacagtcgc gtttgcgact                                                   20


<210>  5
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  5
aatccctatc gattcctgtg g                                                 21


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  6
gaaccggctg atgtgtttgg                                                   20


