                         SEQUENCE LISTING

<110>  National Research Council Of Canada
       National Health Research Institutes
 
<120>  LIPIDATED STREPTOCOCCUS PNEUMONIAE ANTIGEN COMPOSITIONS, METHODS 
       OF PREPARATION AND USE

<130>  39727-027

<140>  15/619,075 
<141>  2017-06-09

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  309
<212>  PRT
<213>  Streptococcus pneumoniae

<400>  1

Met Lys Lys Leu Gly Thr Leu Leu Val Leu Phe Leu Ser Ala Ile Ile 
1               5                   10                  15      


Leu Val Ala Cys Ala Ser Gly Lys Lys Asp Thr Thr Ser Gly Gln Lys 
            20                  25                  30          


Leu Lys Val Val Ala Thr Asn Ser Ile Ile Ala Asp Ile Thr Lys Asn 
        35                  40                  45              


Ile Ala Gly Asp Lys Ile Asp Leu His Ser Ile Val Pro Ile Gly Gln 
    50                  55                  60                  


Asp Pro His Glu Tyr Glu Pro Leu Pro Glu Asp Val Lys Lys Thr Ser 
65                  70                  75                  80  


Glu Ala Asp Leu Ile Phe Tyr Asn Gly Ile Asn Leu Glu Thr Gly Gly 
                85                  90                  95      


Asn Ala Trp Phe Thr Lys Leu Val Glu Asn Ala Lys Lys Thr Glu Asn 
            100                 105                 110         


Lys Asp Tyr Phe Ala Val Ser Asp Gly Val Asp Val Ile Tyr Leu Glu 
        115                 120                 125             


Gly Gln Asn Glu Lys Gly Lys Glu Asp Pro His Ala Trp Leu Asn Leu 
    130                 135                 140                 


Glu Asn Gly Ile Ile Phe Ala Lys Asn Ile Ala Lys Gln Leu Ser Ala 
145                 150                 155                 160 


Lys Asp Pro Asn Asn Lys Glu Phe Tyr Glu Lys Asn Leu Lys Glu Tyr 
                165                 170                 175     


Thr Asp Lys Leu Asp Lys Leu Asp Lys Glu Ser Lys Asp Lys Phe Asn 
            180                 185                 190         


Lys Ile Pro Ala Glu Lys Lys Leu Ile Val Thr Ser Glu Gly Ala Phe 
        195                 200                 205             


Lys Tyr Phe Ser Lys Ala Tyr Gly Val Pro Ser Ala Tyr Ile Trp Glu 
    210                 215                 220                 


Ile Asn Thr Glu Glu Glu Gly Thr Pro Glu Gln Ile Lys Thr Leu Val 
225                 230                 235                 240 


Glu Lys Leu Arg Gln Thr Lys Val Pro Ser Leu Phe Val Glu Ser Ser 
                245                 250                 255     


Val Asp Asp Arg Pro Met Lys Thr Val Ser Gln Asp Thr Asn Ile Pro 
            260                 265                 270         


Ile Tyr Ala Gln Ile Phe Thr Asp Ser Ile Ala Glu Gln Gly Lys Glu 
        275                 280                 285             


Gly Asp Ser Tyr Tyr Ser Met Met Lys Tyr Asn Leu Asp Lys Ile Ala 
    290                 295                 300                 


Glu Gly Leu Ala Lys 
305                 


<210>  2
<211>  290
<212>  PRT
<213>  Streptococcus pneumoniae

<400>  2

Met Ala Ser Gly Lys Lys Asp Thr Thr Ser Gly Gln Lys Leu Lys Val 
1               5                   10                  15      


Val Ala Thr Asn Ser Ile Ile Ala Asp Ile Thr Lys Asn Ile Ala Gly 
            20                  25                  30          


Asp Lys Ile Asp Leu His Ser Ile Val Pro Ile Gly Gln Asp Pro His 
        35                  40                  45              


Glu Tyr Glu Pro Leu Pro Glu Asp Val Lys Lys Thr Ser Glu Ala Asp 
    50                  55                  60                  


Leu Ile Phe Tyr Asn Gly Ile Asn Leu Glu Thr Gly Gly Asn Ala Trp 
65                  70                  75                  80  


Phe Thr Lys Leu Val Glu Asn Ala Lys Lys Thr Glu Asn Lys Asp Tyr 
                85                  90                  95      


Phe Ala Val Ser Asp Gly Val Asp Val Ile Tyr Leu Glu Gly Gln Asn 
            100                 105                 110         


Glu Lys Gly Lys Glu Asp Pro His Ala Trp Leu Asn Leu Glu Asn Gly 
        115                 120                 125             


Ile Ile Phe Ala Lys Asn Ile Ala Lys Gln Leu Ser Ala Lys Asp Pro 
    130                 135                 140                 


Asn Asn Lys Glu Phe Tyr Glu Lys Asn Leu Lys Glu Tyr Thr Asp Lys 
145                 150                 155                 160 


Leu Asp Lys Leu Asp Lys Glu Ser Lys Asp Lys Phe Asn Lys Ile Pro 
                165                 170                 175     


Ala Glu Lys Lys Leu Ile Val Thr Ser Glu Gly Ala Phe Lys Tyr Phe 
            180                 185                 190         


Ser Lys Ala Tyr Gly Val Pro Ser Ala Tyr Ile Trp Glu Ile Asn Thr 
        195                 200                 205             


Glu Glu Glu Gly Thr Pro Glu Gln Ile Lys Thr Leu Val Glu Lys Leu 
    210                 215                 220                 


Arg Gln Thr Lys Val Pro Ser Leu Phe Val Glu Ser Ser Val Asp Asp 
225                 230                 235                 240 


Arg Pro Met Lys Thr Val Ser Gln Asp Thr Asn Ile Pro Ile Tyr Ala 
                245                 250                 255     


Gln Ile Phe Thr Asp Ser Ile Ala Glu Gln Gly Lys Glu Gly Asp Ser 
            260                 265                 270         


Tyr Tyr Ser Met Met Lys Tyr Asn Leu Asp Lys Ile Ala Glu Gly Leu 
        275                 280                 285             


Ala Lys 
    290 


<210>  3
<211>  379
<212>  PRT
<213>  Streptococcus pneumoniae

<400>  3

Met Glu Glu Ser Pro Val Ala Ser Gln Ser Lys Ala Glu Lys Asp Tyr 
1               5                   10                  15      


Asp Ala Ala Lys Lys Asp Ala Lys Asn Ala Lys Lys Ala Val Glu Asp 
            20                  25                  30          


Ala Gln Lys Ala Leu Asp Asp Ala Lys Ala Ala Gln Lys Lys Tyr Asp 
        35                  40                  45              


Glu Asp Gln Lys Lys Thr Glu Glu Lys Ala Ala Leu Glu Lys Ala Ala 
    50                  55                  60                  


Ser Glu Glu Met Asp Lys Ala Val Ala Ala Val Gln Gln Ala Tyr Leu 
65                  70                  75                  80  


Ala Tyr Gln Gln Ala Thr Asp Lys Ala Ala Lys Asp Ala Ala Asp Lys 
                85                  90                  95      


Met Ile Asp Glu Ala Lys Lys Arg Glu Glu Glu Ala Lys Thr Lys Phe 
            100                 105                 110         


Asn Thr Val Arg Ala Met Val Val Pro Glu Pro Glu Gln Leu Ala Glu 
        115                 120                 125             


Thr Lys Lys Lys Ser Glu Glu Ala Lys Gln Lys Ala Pro Glu Leu Thr 
    130                 135                 140                 


Lys Lys Leu Glu Glu Ala Lys Ala Lys Leu Glu Glu Ala Glu Lys Lys 
145                 150                 155                 160 


Ala Thr Glu Ala Lys Gln Lys Val Asp Ala Glu Glu Val Ala Pro Gln 
                165                 170                 175     


Ala Lys Ile Ala Glu Leu Glu Asn Gln Val His Arg Leu Glu Gln Glu 
            180                 185                 190         


Leu Lys Glu Ile Asp Glu Ser Glu Ser Glu Asp Tyr Ala Lys Glu Gly 
        195                 200                 205             


Phe Arg Ala Pro Leu Gln Ser Lys Leu Asp Ala Lys Lys Ala Lys Leu 
    210                 215                 220                 


Ser Lys Leu Glu Glu Leu Ser Asp Lys Ile Asp Glu Leu Asp Ala Glu 
225                 230                 235                 240 


Ile Ala Lys Leu Glu Asp Gln Leu Lys Ala Ala Glu Glu Asn Asn Asn 
                245                 250                 255     


Val Glu Asp Tyr Phe Lys Glu Gly Leu Glu Lys Thr Ile Ala Ala Lys 
            260                 265                 270         


Lys Ala Glu Leu Glu Lys Thr Glu Ala Asp Leu Lys Lys Ala Val Asn 
        275                 280                 285             


Glu Pro Glu Lys Pro Ala Pro Ala Pro Glu Thr Pro Ala Pro Glu Ala 
    290                 295                 300                 


Pro Ala Glu Gln Pro Lys Pro Ala Pro Ala Pro Gln Pro Ala Pro Ala 
305                 310                 315                 320 


Pro Lys Pro Glu Lys Pro Ala Glu Gln Pro Lys Pro Glu Lys Thr Asp 
                325                 330                 335     


Asp Gln Gln Ala Glu Glu Asp Tyr Ala Arg Arg Ser Glu Glu Glu Tyr 
            340                 345                 350         


Asn Arg Leu Thr Gln Gln Gln Pro Pro Lys Ala Glu Lys Pro Ala Pro 
        355                 360                 365             


Ala Pro Lys Leu Glu His His His His His His 
    370                 375                 


<210>  4
<211>  490
<212>  PRT
<213>  Streptococcus pneumoniae

<400>  4

Met Phe Ala Ser Lys Ser Glu Arg Lys Val His Tyr Ser Ile Arg Lys 
1               5                   10                  15      


Phe Ser Ile Gly Val Ala Ser Val Ala Val Ala Ser Leu Val Met Gly 
            20                  25                  30          


Ser Val Val His Ala Thr Glu Asn Glu Gly Ser Thr Gln Ala Ala Thr 
        35                  40                  45              


Ser Ser Asn Met Ala Lys Thr Glu His Arg Lys Ala Ala Lys Gln Val 
    50                  55                  60                  


Val Asp Glu Tyr Ile Glu Lys Met Leu Arg Glu Ile Gln Leu Asp Arg 
65                  70                  75                  80  


Arg Lys His Thr Gln Asn Val Ala Leu Asn Ile Lys Leu Ser Ala Ile 
                85                  90                  95      


Lys Thr Lys Tyr Leu Arg Glu Leu Asn Val Leu Glu Glu Lys Ser Lys 
            100                 105                 110         


Asp Glu Leu Pro Ser Glu Ile Lys Ala Lys Leu Asp Ala Ala Phe Glu 
        115                 120                 125             


Lys Phe Lys Lys Asp Thr Leu Lys Pro Gly Glu Lys Val Ala Glu Ala 
    130                 135                 140                 


Lys Lys Lys Val Glu Glu Ala Lys Lys Lys Ala Glu Asp Gln Lys Glu 
145                 150                 155                 160 


Glu Asp Arg Arg Asn Tyr Pro Thr Asn Thr Tyr Lys Thr Leu Glu Leu 
                165                 170                 175     


Glu Ile Ala Glu Phe Asp Val Lys Val Lys Glu Ala Glu Leu Glu Leu 
            180                 185                 190         


Val Lys Glu Glu Ala Lys Glu Ser Arg Asn Glu Gly Thr Ile Lys Gln 
        195                 200                 205             


Ala Lys Glu Lys Val Glu Ser Lys Lys Ala Glu Ala Thr Arg Leu Glu 
    210                 215                 220                 


Asn Ile Lys Thr Asp Arg Lys Lys Ala Glu Glu Glu Ala Lys Arg Lys 
225                 230                 235                 240 


Ala Asp Ala Lys Leu Lys Glu Ala Asn Val Ala Thr Ser Asp Gln Gly 
                245                 250                 255     


Lys Pro Lys Gly Arg Ala Lys Arg Gly Val Pro Gly Glu Leu Ala Thr 
            260                 265                 270         


Pro Asp Lys Lys Glu Asn Asp Ala Lys Ser Ser Asp Ser Ser Val Gly 
        275                 280                 285             


Glu Glu Thr Leu Pro Ser Ser Ser Leu Lys Ser Gly Lys Lys Val Ala 
    290                 295                 300                 


Glu Ala Glu Lys Lys Val Glu Glu Ala Glu Lys Lys Ala Lys Asp Gln 
305                 310                 315                 320 


Lys Glu Glu Asp Arg Arg Asn Tyr Pro Thr Asn Thr Tyr Lys Thr Leu 
                325                 330                 335     


Asp Leu Glu Ile Ala Glu Ser Asp Val Lys Val Lys Glu Ala Glu Leu 
            340                 345                 350         


Glu Leu Val Lys Glu Glu Ala Lys Glu Pro Arg Asp Glu Glu Lys Ile 
        355                 360                 365             


Lys Gln Ala Lys Ala Lys Val Glu Ser Lys Lys Ala Glu Ala Thr Arg 
    370                 375                 380                 


Leu Glu Asn Ile Lys Thr Asp Arg Lys Lys Ala Glu Glu Glu Ala Lys 
385                 390                 395                 400 


Arg Lys Ala Ala Glu Glu Asp Lys Val Lys Glu Lys Pro Ala Glu Gln 
                405                 410                 415     


Pro Gln Pro Ala Pro Ala Thr Gln Pro Glu Lys Pro Ala Pro Lys Pro 
            420                 425                 430         


Glu Lys Pro Ala Glu Gln Pro Lys Ala Glu Lys Thr Asp Asp Gln Gln 
        435                 440                 445             


Ala Glu Glu Asp Tyr Ala Arg Arg Ser Glu Glu Glu Tyr Asn Arg Leu 
    450                 455                 460                 


Thr Gln Gln Gln Pro Pro Lys Thr Glu Lys Pro Ala Gln Pro Ser Thr 
465                 470                 475                 480 


Pro Lys Leu Glu His His His His His His 
                485                 490 


<210>  5
<211>  20
<212>  PRT
<213>  Streptococcus pneumoniae

<400>  5

Met Lys Lys Leu Gly Thr Leu Leu Val Leu Phe Leu Ser Ala Ile Ile 
1               5                   10                  15      


Leu Val Ala Cys 
            20  


<210>  6
<211>  954
<212>  DNA
<213>  Streptococcus pneumoniae

<400>  6
atgaaaaaac tgggcaccct gctggtgctg tttctgagcg cgattattct ggtggcgtgc       60

gcgagcggca aaaaagatac caccagcggc cagaaactga aagtggtggc gaccaacagc      120

attattgcgg atattaccaa aaacattgcg ggcgataaaa ttgatctgca tagcattgtg      180

ccgattggcc aggatccgca tgaatatgaa ccgctgccgg aagatgtgaa aaaaaccagc      240

gaagcggatc tgatttttta taacggcatt aacctggaaa ccggcggcaa cgcgtggttt      300

accaaactgg tggaaaacgc gaaaaaaacc gaaaacaaag attattttgc ggtgagcgat      360

ggcgtggatg tgatttatct ggaaggccag aacgaaaaag gcaaagaaga tccgcatgcg      420

tggctgaacc tggaaaacgg cattattttt gcgaaaaaca ttgcgaaaca gctgagcgcg      480

aaagatccga acaacaaaga attttatgaa aaaaacctga aagaatatac cgataaactg      540

gataaactgg ataaagaaag caaagataaa tttaacaaaa ttccggcgga aaaaaaactg      600

attgtgacca gcgaaggcgc gtttaaatat tttagcaaag cgtatggcgt gccgagcgcg      660

tatatttggg aaattaacac cgaagaagaa ggcaccccgg aacagattaa aaccctggtg      720

gaaaaactgc gtcagaccaa agtgccgagc ctgtttgtgg aaagcagcgt ggatgatcgt      780

ccgatgaaaa ccgtgagcca ggataccaac attccgattt atgcgcagat ttttaccgat      840

agcattgcgg aacagggcaa agaaggcgat agctattata gcatgatgaa atataacctg      900

gataaaattg cggaaggcct ggcgaaactc gagcaccacc accaccacca ctga            954


<210>  7
<211>  315
<212>  PRT
<213>  Streptococcus pneumoniae

<400>  7

Met Lys Lys Leu Gly Thr Leu Leu Val Leu Phe Leu Ser Ala Ile Ile 
1               5                   10                  15      


Leu Val Ala Cys Ala Ser Gly Lys Lys Asp Thr Thr Ser Gly Gln Lys 
            20                  25                  30          


Leu Lys Val Val Ala Thr Asn Ser Ile Ile Ala Asp Ile Thr Lys Asn 
        35                  40                  45              


Ile Ala Gly Asp Lys Ile Asp Leu His Ser Ile Val Pro Ile Gly Gln 
    50                  55                  60                  


Asp Pro His Glu Tyr Glu Pro Leu Pro Glu Asp Val Lys Lys Thr Ser 
65                  70                  75                  80  


Glu Ala Asp Leu Ile Phe Tyr Asn Gly Ile Asn Leu Glu Thr Gly Gly 
                85                  90                  95      


Asn Ala Trp Phe Thr Lys Leu Val Glu Asn Ala Lys Lys Thr Glu Asn 
            100                 105                 110         


Lys Asp Tyr Phe Ala Val Ser Asp Gly Val Asp Val Ile Tyr Leu Glu 
        115                 120                 125             


Gly Gln Asn Glu Lys Gly Lys Glu Asp Pro His Ala Trp Leu Asn Leu 
    130                 135                 140                 


Glu Asn Gly Ile Ile Phe Ala Lys Asn Ile Ala Lys Gln Leu Ser Ala 
145                 150                 155                 160 


Lys Asp Pro Asn Asn Lys Glu Phe Tyr Glu Lys Asn Leu Lys Glu Tyr 
                165                 170                 175     


Thr Asp Lys Leu Asp Lys Leu Asp Lys Glu Ser Lys Asp Lys Phe Asn 
            180                 185                 190         


Lys Ile Pro Ala Glu Lys Lys Leu Ile Val Thr Ser Glu Gly Ala Phe 
        195                 200                 205             


Lys Tyr Phe Ser Lys Ala Tyr Gly Val Pro Ser Ala Tyr Ile Trp Glu 
    210                 215                 220                 


Ile Asn Thr Glu Glu Glu Gly Thr Pro Glu Gln Ile Lys Thr Leu Val 
225                 230                 235                 240 


Glu Lys Leu Arg Gln Thr Lys Val Pro Ser Leu Phe Val Glu Ser Ser 
                245                 250                 255     


Val Asp Asp Arg Pro Met Lys Thr Val Ser Gln Asp Thr Asn Ile Pro 
            260                 265                 270         


Ile Tyr Ala Gln Ile Phe Thr Asp Ser Ile Ala Glu Gln Gly Lys Glu 
        275                 280                 285             


Gly Asp Ser Tyr Tyr Ser Met Met Lys Tyr Asn Leu Asp Lys Ile Ala 
    290                 295                 300                 


Glu Gly Leu Ala Lys His His His His His His 
305                 310                 315 


<210>  8
<211>  296
<212>  PRT
<213>  Streptococcus pneumoniae

<400>  8

Met Ala Ser Gly Lys Lys Asp Thr Thr Ser Gly Gln Lys Leu Lys Val 
1               5                   10                  15      


Val Ala Thr Asn Ser Ile Ile Ala Asp Ile Thr Lys Asn Ile Ala Gly 
            20                  25                  30          


Asp Lys Ile Asp Leu His Ser Ile Val Pro Ile Gly Gln Asp Pro His 
        35                  40                  45              


Glu Tyr Glu Pro Leu Pro Glu Asp Val Lys Lys Thr Ser Glu Ala Asp 
    50                  55                  60                  


Leu Ile Phe Tyr Asn Gly Ile Asn Leu Glu Thr Gly Gly Asn Ala Trp 
65                  70                  75                  80  


Phe Thr Lys Leu Val Glu Asn Ala Lys Lys Thr Glu Asn Lys Asp Tyr 
                85                  90                  95      


Phe Ala Val Ser Asp Gly Val Asp Val Ile Tyr Leu Glu Gly Gln Asn 
            100                 105                 110         


Glu Lys Gly Lys Glu Asp Pro His Ala Trp Leu Asn Leu Glu Asn Gly 
        115                 120                 125             


Ile Ile Phe Ala Lys Asn Ile Ala Lys Gln Leu Ser Ala Lys Asp Pro 
    130                 135                 140                 


Asn Asn Lys Glu Phe Tyr Glu Lys Asn Leu Lys Glu Tyr Thr Asp Lys 
145                 150                 155                 160 


Leu Asp Lys Leu Asp Lys Glu Ser Lys Asp Lys Phe Asn Lys Ile Pro 
                165                 170                 175     


Ala Glu Lys Lys Leu Ile Val Thr Ser Glu Gly Ala Phe Lys Tyr Phe 
            180                 185                 190         


Ser Lys Ala Tyr Gly Val Pro Ser Ala Tyr Ile Trp Glu Ile Asn Thr 
        195                 200                 205             


Glu Glu Glu Gly Thr Pro Glu Gln Ile Lys Thr Leu Val Glu Lys Leu 
    210                 215                 220                 


Arg Gln Thr Lys Val Pro Ser Leu Phe Val Glu Ser Ser Val Asp Asp 
225                 230                 235                 240 


Arg Pro Met Lys Thr Val Ser Gln Asp Thr Asn Ile Pro Ile Tyr Ala 
                245                 250                 255     


Gln Ile Phe Thr Asp Ser Ile Ala Glu Gln Gly Lys Glu Gly Asp Ser 
            260                 265                 270         


Tyr Tyr Ser Met Met Lys Tyr Asn Leu Asp Lys Ile Ala Glu Gly Leu 
        275                 280                 285             


Ala Lys His His His His His His 
    290                 295     


<210>  9
<211>  290
<212>  PRT
<213>  Streptococcus pneumoniae

<400>  9

Cys Ala Ser Gly Lys Lys Asp Thr Thr Ser Gly Gln Lys Leu Lys Val 
1               5                   10                  15      


Val Ala Thr Asn Ser Ile Ile Ala Asp Ile Thr Lys Asn Ile Ala Gly 
            20                  25                  30          


Asp Lys Ile Asp Leu His Ser Ile Val Pro Ile Gly Gln Asp Pro His 
        35                  40                  45              


Glu Tyr Glu Pro Leu Pro Glu Asp Val Lys Lys Thr Ser Glu Ala Asp 
    50                  55                  60                  


Leu Ile Phe Tyr Asn Gly Ile Asn Leu Glu Thr Gly Gly Asn Ala Trp 
65                  70                  75                  80  


Phe Thr Lys Leu Val Glu Asn Ala Lys Lys Thr Glu Asn Lys Asp Tyr 
                85                  90                  95      


Phe Ala Val Ser Asp Gly Val Asp Val Ile Tyr Leu Glu Gly Gln Asn 
            100                 105                 110         


Glu Lys Gly Lys Glu Asp Pro His Ala Trp Leu Asn Leu Glu Asn Gly 
        115                 120                 125             


Ile Ile Phe Ala Lys Asn Ile Ala Lys Gln Leu Ser Ala Lys Asp Pro 
    130                 135                 140                 


Asn Asn Lys Glu Phe Tyr Glu Lys Asn Leu Lys Glu Tyr Thr Asp Lys 
145                 150                 155                 160 


Leu Asp Lys Leu Asp Lys Glu Ser Lys Asp Lys Phe Asn Lys Ile Pro 
                165                 170                 175     


Ala Glu Lys Lys Leu Ile Val Thr Ser Glu Gly Ala Phe Lys Tyr Phe 
            180                 185                 190         


Ser Lys Ala Tyr Gly Val Pro Ser Ala Tyr Ile Trp Glu Ile Asn Thr 
        195                 200                 205             


Glu Glu Glu Gly Thr Pro Glu Gln Ile Lys Thr Leu Val Glu Lys Leu 
    210                 215                 220                 


Arg Gln Thr Lys Val Pro Ser Leu Phe Val Glu Ser Ser Val Asp Asp 
225                 230                 235                 240 


Arg Pro Met Lys Thr Val Ser Gln Asp Thr Asn Ile Pro Ile Tyr Ala 
                245                 250                 255     


Gln Ile Phe Thr Asp Ser Ile Ala Glu Gln Gly Lys Glu Gly Asp Ser 
            260                 265                 270         


Tyr Tyr Ser Met Met Lys Tyr Asn Leu Asp Lys Ile Ala Glu Gly Leu 
        275                 280                 285             


Ala Lys 
    290 




