                         SEQUENCE LISTING

<110>  Chan, Eugene Y.
 
<120>  Fluorogenic peptide substrates for in solution and solid phase 
       factor Xa measurements

<130>  DNM-121PC

<140>  PCT/US18/035215
<141>  2018-05-30

<150>  62/512,443
<151>  2017-05-30

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Arg has a carbamate N-terminal group

<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Arg is DArg

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Arg has a fluorophore attachment group of 
       7-amino-4-carbamoylmethyl-coumarin, the fluorophore attachment 
       group has a polyethylene glycol linker of PEG2, and the 
       polyethylene glycol linker has an attachment group NH2

<400>  1

Arg Gly Arg 
1           


<210>  2
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Ile has a succinate N-terminal group

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Glu is Glu(gamma-pip)

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Arg has a fluorophore attachment group of 
       7-amino-4-carbamoylmethyl-coumarin, the fluorophore attachment 
       group has a polyethylene glycol linker of PEG2, and the 
       polyethylene glycol linker has an attachment group NH2

<400>  2

Ile Glu Gly Arg 
1               


<210>  3
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Glu is Glu(gamma-pip)

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Arg has a fluorophore attachment group of 
       7-amino-4-9(trifluoromethyl)-coumarin

<400>  3

Lys Glu Ile Glu Gly Arg 
1               5       


<210>  4
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Ile has a succinate N-terminal group

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Glu is Glu(gamma-pip)

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Arg has a fluorophore attachment group of 
       7-amino-4-9(trifluoromethyl)-coumarin

<400>  4

Ile Glu Gly Arg 
1               


<210>  5
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic seqence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Ile has a succinate N-terminal group

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Glu is Glu(gamma-pip)

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Arg has a fluorophore attachment group of 
       7-amino-4-carbamoylmethyl-coumarin, the fluorophore attachment 
       group has a polyethylene glycol linker of PEG2, and the 
       polyethylene glycol linker has an attachment group NH2

<400>  5

Ile Glu Gly Arg 
1               


<210>  6
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Ile has a succinate N-terminal group

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Glu is Glu(gamma-pip)

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Arg has a fluorophore attachment group of 
       7-amino-4-carbamoylmethyl-coumarin, the fluorophore attachment 
       group has a polyethylene glycol linker of PEG4, and the 
       polyethylene glycol linker has an attachment group NH2

<400>  6

Ile Glu Gly Arg 
1               


<210>  7
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Ile has a succinate N-terminal group

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Glu is Glu(gamma-pip)

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Arg has a fluorophore attachment group of 
       7-amino-4-carbamoylmethyl-coumarin, the fluorophore attachment 
       group has a polyethylene glycol linker of PEG4, and the 
       polyethylene glycol linker has an attachment group biotin

<400>  7

Ile Glu Gly Arg 
1               


<210>  8
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Ile has a benzyl carbamate N-terminal group

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Glu is Glu(gamma-OR) and R is H or CH3

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Arg has a fluorophore attachment group of 
       7-amino-4-carbamoylmethyl-coumarin

<400>  8

Ile Glu Gly Arg 
1               


<210>  9
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Arg is DArg

<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Arg has a carbamate N-terminal group

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Arg has a fluorophore attachment group of 
       7-amino-4-methylcoumarin

<400>  9

Arg Gly Arg 
1           


<210>  10
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Ile has a succinate N-terminal group

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Glu is Glu(gamma-pip)

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Arg has a fluorophore attachment group of 
       7-amino-4-methylcoumarin

<400>  10

Ile Glu Gly Arg 
1               


<210>  11
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Arg is DArg

<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Arg has an N-terminal group of carbamate, succinate, benzyl 
       carbamate, N-alpha-benzyloxycarbonyl or lysine

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Arg has a fluorophore attachment group of 
       7-amino-4-carbamoylmethyl-coumarin, 
       7-amino-4-9(trifluoromethyl)-coumarin, or 
       7-amino-4-methylcoumarin

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  The fluorophore attachment group has a linker of PEG8, PEG(n) or 
       C(n) where n is an integer 0, 1, 2, 3, or 4

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  The linker to the fluorophore attachment group has an attachment 
       group NH2, biotin, COOH, SH, SCM, acrylate, click, maleimide, 
       alkyne, ITC, NHS, SMCC, ALD, EPOX, ester, hydrazide, OH, SIL, or 
       VA

<400>  11

Arg Gly Arg 
1           


<210>  12
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Arg is DArg

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Arg has a fluorophore attachment group of 
       7-amino-4-carbamoylmethyl-coumarin, 
       7-amino-4-9(trifluoromethyl)-coumarin, or 
       7-amino-4-methylcoumarin

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  The fluorophore attachment group has a linker of PEG8, PEG(n) or 
       C(n) where n is an integer 0, 1, 2, 3, or 4

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  The linker to the fluorophore attachment group has an attachment 
       group NH2, biotin, COOH, SH, SCM, acrylate, click, maleimide, 
       alkyne, ITC, NHS, SMCC, ALD, EPOX, ester, hydrazide, OH, SIL, or 
       VA

<400>  12

Arg Gly Arg 
1           


<210>  13
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Arg is DArg

<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Arg has an N-terminal group of carbamate, succinate, benzyl 
       carbamate, N-alpha-benzyloxycarbonyl or lysine

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Arg has a fluorophore attachment group of 
       7-amino-4-carbamoylmethyl-coumarin, 
       7-amino-4-9(trifluoromethyl)-coumarin, or 
       7-amino-4-methylcoumarin

<400>  13

Arg Gly Arg 
1           


<210>  14
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Arg is DArg

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Arg has a fluorophore attachment group of 
       7-amino-4-carbamoylmethyl-coumarin, 
       7-amino-4-9(trifluoromethyl)-coumarin, or 
       7-amino-4-methylcoumarin

<400>  14

Arg Gly Arg 
1           


<210>  15
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Ile has an N-terminal group of carbamate, succinate, benzyl 
       carbamate, N-alpha-benzyloxycarbonyl or lysine

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa is Asp, Glu, Glu(gamma-pip), or Glu(gamma-OR) and R is H or 
       CH3

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Arg has a fluorophore attachment group of 
       7-amino-4-carbamoylmethyl-coumarin, 
       7-amino-4-9(trifluoromethyl)-coumarin, or 
       7-amino-4-methylcoumarin

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  The fluorophore attachment group has a linker of PEG8, PEG(n) or 
       C(n) where n is an integer 0, 1, 2, 3, or 4

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  The linker to the fluorophore attachment group has an attachment 
       group NH2, biotin, COOH, SH, SCM, acrylate, click, maleimide, 
       alkyne, ITC, NHS, SMCC, ALD, EPOX, ester, hydrazide, OH, SIL, or 
       VA

<400>  15

Ile Xaa Gly Arg 
1               


<210>  16
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Ile has an N-terminal group of carbamate, succinate, benzyl 
       carbamate, N-alpha-benzyloxycarbonyl or lysine

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa is Asp, Glu, Glu(gamma-pip), or Glu(gamma-OR) and R is H or 
       CH3

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Arg has a fluorophore attachment group of 
       7-amino-4-carbamoylmethyl-coumarin, 
       7-amino-4-9(trifluoromethyl)-coumarin, or 
       7-amino-4-methylcoumarin

<400>  16

Ile Xaa Gly Arg 
1               


<210>  17
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa is Asp, Glu, Glu(gamma-pip), or Glu(gamma-OR) and R is H or 
       CH3

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Arg has a fluorophore attachment group of 
       7-amino-4-carbamoylmethyl-coumarin, 
       7-amino-4-9(trifluoromethyl)-coumarin, or 
       7-amino-4-methylcoumarin

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  The fluorophore attachment group has a linker of PEG8, PEG(n) or 
       C(n) where n is an integer 0, 1, 2, 3, or 4

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  The linker to the fluorophore attachment group has an attachment 
       group NH2, biotin, COOH, SH, SCM, acrylate, click, maleimide, 
       alkyne, ITC, NHS, SMCC, ALD, EPOX, ester, hydrazide, OH, SIL, or 
       VA

<400>  17

Ile Xaa Gly Arg 
1               


<210>  18
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa is Asp, Glu, Glu(gamma-pip), or Glu(gamma-OR) and R is H or 
       CH3

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Arg has a fluorophore attachment group of 
       7-amino-4-carbamoylmethyl-coumarin, 
       7-amino-4-9(trifluoromethyl)-coumarin, or 
       7-amino-4-methylcoumarin

<400>  18

Ile Xaa Gly Arg 
1               


