                         SEQUENCE LISTING

<110>  Nejatollahi, Foroogh
 
<120>  ANTI-MUC18 IMMUNOTOXIN AND APPLICATIONS THEREOF

<130>  ----

<150>  US 62/512195,
<151>  2017-05-30

<160>  25    

<170>  PatentIn version 3.5

<210>  1
<211>  1530
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of human anti-MUC18 immunotoxin


<220>
<221>  CDS
<222>  (1)..(1530)

<400>  1
ggc cga ggt gca ctg gtg gag tct ggc cca gga ctg gtg aag cct tcg         48
Gly Arg Gly Ala Leu Val Glu Ser Gly Pro Gly Leu Val Lys Pro Ser           
1               5                   10                  15                

gag acc ctg ttc ctc acc tgc act gtc tct ggt ggc tcc atc agt tct         96
Glu Thr Leu Phe Leu Thr Cys Thr Val Ser Gly Gly Ser Ile Ser Ser           
            20                  25                  30                    

tac tac tgg agc tgg atc cgg cag ccc gca ggg aag gga ctg gag tgg        144
Tyr Tyr Trp Ser Trp Ile Arg Gln Pro Ala Gly Lys Gly Leu Glu Trp           
        35                  40                  45                        

att gcg tat atc tat tac agt ggg agc acc aac tac aac ccc tcc ctc        192
Ile Ala Tyr Ile Tyr Tyr Ser Gly Ser Thr Asn Tyr Asn Pro Ser Leu           
    50                  55                  60                            

aag agt cga gtc acc ata tca gta gac acg tcc aac aac cag ttc tcc        240
Lys Ser Arg Val Thr Ile Ser Val Asp Thr Ser Asn Asn Gln Phe Ser           
65                  70                  75                  80            

ctg aaa ctg agc tct gtg acc gct gcg gac acg gcc gtg tat tat tgt        288
Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys           
                85                  90                  95                

gcg aag tta aca gca gcc ggg ggc cac ttc gac ccc tgg ggc cag ggc        336
Ala Lys Leu Thr Ala Ala Gly Gly His Phe Asp Pro Trp Gly Gln Gly           
            100                 105                 110                   

acc ctg gtc acc gtc tcc tca ggt gga ggc ggt tca ggc gga ggt ggc        384
Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly           
        115                 120                 125                       

agc ggc ggt ggc gga tcg gac atc gtg atg acc cag tct cca tcc tcc        432
Ser Gly Gly Gly Gly Ser Asp Ile Val Met Thr Gln Ser Pro Ser Ser           
    130                 135                 140                           

ctg tct gca tct ata ggg gac aga gtc acc atc act tgt cgg gca agt        480
Leu Ser Ala Ser Ile Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser           
145                 150                 155                 160           

cag ggc att agc aat ttt tta gcc tgg ttt cag cag aaa cca ggg aaa        528
Gln Gly Ile Ser Asn Phe Leu Ala Trp Phe Gln Gln Lys Pro Gly Lys           
                165                 170                 175               

gcc cct aag tcc ctg atc tat gct gca tcc agt ttg caa agt ggg gtc        576
Ala Pro Lys Ser Leu Ile Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val           
            180                 185                 190                   

cca tca aag ttc agc ggc agt gga tct ggg aca gat ttc act ctc gcc        624
Pro Ser Lys Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Ala           
        195                 200                 205                       

atc agc agc ctg cag cct gaa gat ttt gca act tat tac tgt ctc caa        672
Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln           
    210                 215                 220                           

gat tcc gat tat cct ctc act ttc ggc gga ggg acc aag ctg gag atc        720
Asp Ser Asp Tyr Pro Leu Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile           
225                 230                 235                 240           

aaa cgt gct agc ggc ggc ccg gaa ggc ggc agc cgc cat cgt cag ccg        768
Lys Arg Ala Ser Gly Gly Pro Glu Gly Gly Ser Arg His Arg Gln Pro           
                245                 250                 255               

cgt ggc tgg gaa cag ctg gca gac gtg gtt tcc ctg acc tgt ccg gtt        816
Arg Gly Trp Glu Gln Leu Ala Asp Val Val Ser Leu Thr Cys Pro Val           
            260                 265                 270                   

gct gcg ggc gaa tgt gcg ggt ccg gca gat tcc ggt gat gca ctg ctg        864
Ala Ala Gly Glu Cys Ala Gly Pro Ala Asp Ser Gly Asp Ala Leu Leu           
        275                 280                 285                       

gaa cgt aac tat ccg acc ggc gcc gaa ttt ctg ggt gat ggc ggt gca        912
Glu Arg Asn Tyr Pro Thr Gly Ala Glu Phe Leu Gly Asp Gly Gly Ala           
    290                 295                 300                           

att tca ttc tcg acc cgc ggc acg cag aat tgg acg gtg gaa cgt ctg        960
Ile Ser Phe Ser Thr Arg Gly Thr Gln Asn Trp Thr Val Glu Arg Leu           
305                 310                 315                 320           

ctg caa gcc cat cgc caa ctg gaa gaa ggc ggt tat gtt ttt gtc ggc       1008
Leu Gln Ala His Arg Gln Leu Glu Glu Gly Gly Tyr Val Phe Val Gly           
                325                 330                 335               

tat cat ggc acc ttt ctg gaa gcc gca cag tca atc gtg ttt ggc ggt       1056
Tyr His Gly Thr Phe Leu Glu Ala Ala Gln Ser Ile Val Phe Gly Gly           
            340                 345                 350                   

gtt cgt gcg cgc tcg cag gat ctg gat gct att tgg gcg ggc ttc tac       1104
Val Arg Ala Arg Ser Gln Asp Leu Asp Ala Ile Trp Ala Gly Phe Tyr           
        355                 360                 365                       

atc gcc ggt gat ccg gcg ctg gcg tat ggc tac gca cag gat caa gaa       1152
Ile Ala Gly Asp Pro Ala Leu Ala Tyr Gly Tyr Ala Gln Asp Gln Glu           
    370                 375                 380                           

ccg gac gct gcg ggc cgt att cgc aac ggt gcg ctg ctg cgt gtg tat       1200
Pro Asp Ala Ala Gly Arg Ile Arg Asn Gly Ala Leu Leu Arg Val Tyr           
385                 390                 395                 400           

gtt ccg cgc agc tct ctg ccg ggc ttt tat gca acc ggt ctg acg ctg       1248
Val Pro Arg Ser Ser Leu Pro Gly Phe Tyr Ala Thr Gly Leu Thr Leu           
                405                 410                 415               

gcc gca ccg gaa gct gcg ggc gaa gtg gaa cgt ctg att ggt cac ccg       1296
Ala Ala Pro Glu Ala Ala Gly Glu Val Glu Arg Leu Ile Gly His Pro           
            420                 425                 430                   

ctg ccg ctg cgc ctg gat gct atc acc ggt ccg gaa gaa gcg ggc ggt       1344
Leu Pro Leu Arg Leu Asp Ala Ile Thr Gly Pro Glu Glu Ala Gly Gly           
        435                 440                 445                       

cgt ctg gaa acg att ctg ggt tgg ccg ctg gct gaa cgc acc gtc gtg       1392
Arg Leu Glu Thr Ile Leu Gly Trp Pro Leu Ala Glu Arg Thr Val Val           
    450                 455                 460                           

att ccg agc gcg atc ccg acc gat ccg cgt aat gtt ggc ggt gat ctg       1440
Ile Pro Ser Ala Ile Pro Thr Asp Pro Arg Asn Val Gly Gly Asp Leu           
465                 470                 475                 480           

gac ccg agt tcc atc ccg gac tct gaa gcc gca atc tca gcc ctg ccg       1488
Asp Pro Ser Ser Ile Pro Asp Ser Glu Ala Ala Ile Ser Ala Leu Pro           
                485                 490                 495               

gat tat gcc agc caa ccg ggt aaa ccg ccg aaa gac gaa ctg               1530
Asp Tyr Ala Ser Gln Pro Gly Lys Pro Pro Lys Asp Glu Leu                   
            500                 505                 510                   


<210>  2
<211>  510
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  2

Gly Arg Gly Ala Leu Val Glu Ser Gly Pro Gly Leu Val Lys Pro Ser 
1               5                   10                  15      


Glu Thr Leu Phe Leu Thr Cys Thr Val Ser Gly Gly Ser Ile Ser Ser 
            20                  25                  30          


Tyr Tyr Trp Ser Trp Ile Arg Gln Pro Ala Gly Lys Gly Leu Glu Trp 
        35                  40                  45              


Ile Ala Tyr Ile Tyr Tyr Ser Gly Ser Thr Asn Tyr Asn Pro Ser Leu 
    50                  55                  60                  


Lys Ser Arg Val Thr Ile Ser Val Asp Thr Ser Asn Asn Gln Phe Ser 
65                  70                  75                  80  


Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Leu Thr Ala Ala Gly Gly His Phe Asp Pro Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Asp Ile Val Met Thr Gln Ser Pro Ser Ser 
    130                 135                 140                 


Leu Ser Ala Ser Ile Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser 
145                 150                 155                 160 


Gln Gly Ile Ser Asn Phe Leu Ala Trp Phe Gln Gln Lys Pro Gly Lys 
                165                 170                 175     


Ala Pro Lys Ser Leu Ile Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val 
            180                 185                 190         


Pro Ser Lys Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Ala 
        195                 200                 205             


Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln 
    210                 215                 220                 


Asp Ser Asp Tyr Pro Leu Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 
225                 230                 235                 240 


Lys Arg Ala Ser Gly Gly Pro Glu Gly Gly Ser Arg His Arg Gln Pro 
                245                 250                 255     


Arg Gly Trp Glu Gln Leu Ala Asp Val Val Ser Leu Thr Cys Pro Val 
            260                 265                 270         


Ala Ala Gly Glu Cys Ala Gly Pro Ala Asp Ser Gly Asp Ala Leu Leu 
        275                 280                 285             


Glu Arg Asn Tyr Pro Thr Gly Ala Glu Phe Leu Gly Asp Gly Gly Ala 
    290                 295                 300                 


Ile Ser Phe Ser Thr Arg Gly Thr Gln Asn Trp Thr Val Glu Arg Leu 
305                 310                 315                 320 


Leu Gln Ala His Arg Gln Leu Glu Glu Gly Gly Tyr Val Phe Val Gly 
                325                 330                 335     


Tyr His Gly Thr Phe Leu Glu Ala Ala Gln Ser Ile Val Phe Gly Gly 
            340                 345                 350         


Val Arg Ala Arg Ser Gln Asp Leu Asp Ala Ile Trp Ala Gly Phe Tyr 
        355                 360                 365             


Ile Ala Gly Asp Pro Ala Leu Ala Tyr Gly Tyr Ala Gln Asp Gln Glu 
    370                 375                 380                 


Pro Asp Ala Ala Gly Arg Ile Arg Asn Gly Ala Leu Leu Arg Val Tyr 
385                 390                 395                 400 


Val Pro Arg Ser Ser Leu Pro Gly Phe Tyr Ala Thr Gly Leu Thr Leu 
                405                 410                 415     


Ala Ala Pro Glu Ala Ala Gly Glu Val Glu Arg Leu Ile Gly His Pro 
            420                 425                 430         


Leu Pro Leu Arg Leu Asp Ala Ile Thr Gly Pro Glu Glu Ala Gly Gly 
        435                 440                 445             


Arg Leu Glu Thr Ile Leu Gly Trp Pro Leu Ala Glu Arg Thr Val Val 
    450                 455                 460                 


Ile Pro Ser Ala Ile Pro Thr Asp Pro Arg Asn Val Gly Gly Asp Leu 
465                 470                 475                 480 


Asp Pro Ser Ser Ile Pro Asp Ser Glu Ala Ala Ile Ser Ala Leu Pro 
                485                 490                 495     


Asp Tyr Ala Ser Gln Pro Gly Lys Pro Pro Lys Asp Glu Leu 
            500                 505                 510 


<210>  3
<211>  726
<212>  DNA
<213>  Homo sapiens


<220>
<221>  CDS
<222>  (1)..(726)

<400>  3
ggt cga ggt gca ctg gtg gag tct ggc cca gga ctg gtg aag cct tcg         48
Gly Arg Gly Ala Leu Val Glu Ser Gly Pro Gly Leu Val Lys Pro Ser           
1               5                   10                  15                

gag acc ctg ttc ctc acc tgc act gtc tct ggt ggc tcc atc agt tct         96
Glu Thr Leu Phe Leu Thr Cys Thr Val Ser Gly Gly Ser Ile Ser Ser           
            20                  25                  30                    

tac tac tgg agc tgg atc cgg cag ccc gca ggg aag gga ctg gag tgg        144
Tyr Tyr Trp Ser Trp Ile Arg Gln Pro Ala Gly Lys Gly Leu Glu Trp           
        35                  40                  45                        

att gcg tat atc tat tac agt ggg agc acc aac tac aac ccc tcc ctc        192
Ile Ala Tyr Ile Tyr Tyr Ser Gly Ser Thr Asn Tyr Asn Pro Ser Leu           
    50                  55                  60                            

aag agt cga gtc acc ata tca gta gac acg tcc aac aac cag ttc tcc        240
Lys Ser Arg Val Thr Ile Ser Val Asp Thr Ser Asn Asn Gln Phe Ser           
65                  70                  75                  80            

ctg aaa ctg agc tct gtg acc gct gcg gac acg gcc gtg tat tat tgt        288
Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys           
                85                  90                  95                

gcg aag tta aca gca gcc ggg ggc cac ttc gac ccc tgg ggc cag ggc        336
Ala Lys Leu Thr Ala Ala Gly Gly His Phe Asp Pro Trp Gly Gln Gly           
            100                 105                 110                   

acc ctg gtc acc gtc tcc tca ggt gga ggc ggt tca ggc gga ggt ggc        384
Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly           
        115                 120                 125                       

agc ggc ggt ggc gga tcg gac atc gtg atg acc cag tct cca tcc tcc        432
Ser Gly Gly Gly Gly Ser Asp Ile Val Met Thr Gln Ser Pro Ser Ser           
    130                 135                 140                           

ctg tct gca tct ata ggg gac aga gtc acc atc act tgt cgg gca agt        480
Leu Ser Ala Ser Ile Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser           
145                 150                 155                 160           

cag ggc att agc aat ttt tta gcc tgg ttt cag cag aaa cca ggg aaa        528
Gln Gly Ile Ser Asn Phe Leu Ala Trp Phe Gln Gln Lys Pro Gly Lys           
                165                 170                 175               

gcc cct aag tcc ctg atc tat gct gca tcc agt ttg caa agt ggg gtc        576
Ala Pro Lys Ser Leu Ile Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val           
            180                 185                 190                   

cca tca aag ttc agc ggc agt gga tct ggg aca gat ttc act ctc gcc        624
Pro Ser Lys Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Ala           
        195                 200                 205                       

atc agc agc ctg cag cct gaa gat ttt gca act tat tac tgt ctc caa        672
Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln           
    210                 215                 220                           

gat tcc gat tat cct ctc act ttc ggc gga ggg acc aag ctg gag atc        720
Asp Ser Asp Tyr Pro Leu Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile           
225                 230                 235                 240           

aaa cgt                                                                726
Lys Arg                                                                   
                                                                          


<210>  4
<211>  242
<212>  PRT
<213>  Homo sapiens

<400>  4

Gly Arg Gly Ala Leu Val Glu Ser Gly Pro Gly Leu Val Lys Pro Ser 
1               5                   10                  15      


Glu Thr Leu Phe Leu Thr Cys Thr Val Ser Gly Gly Ser Ile Ser Ser 
            20                  25                  30          


Tyr Tyr Trp Ser Trp Ile Arg Gln Pro Ala Gly Lys Gly Leu Glu Trp 
        35                  40                  45              


Ile Ala Tyr Ile Tyr Tyr Ser Gly Ser Thr Asn Tyr Asn Pro Ser Leu 
    50                  55                  60                  


Lys Ser Arg Val Thr Ile Ser Val Asp Thr Ser Asn Asn Gln Phe Ser 
65                  70                  75                  80  


Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Leu Thr Ala Ala Gly Gly His Phe Asp Pro Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Asp Ile Val Met Thr Gln Ser Pro Ser Ser 
    130                 135                 140                 


Leu Ser Ala Ser Ile Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser 
145                 150                 155                 160 


Gln Gly Ile Ser Asn Phe Leu Ala Trp Phe Gln Gln Lys Pro Gly Lys 
                165                 170                 175     


Ala Pro Lys Ser Leu Ile Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val 
            180                 185                 190         


Pro Ser Lys Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Ala 
        195                 200                 205             


Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln 
    210                 215                 220                 


Asp Ser Asp Tyr Pro Leu Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 
225                 230                 235                 240 


Lys Arg 
        


<210>  5
<211>  777
<212>  DNA
<213>  Pseudomonas aeruginosa


<220>
<221>  CDS
<222>  (1)..(777)

<400>  5
cgc cat cgt cag ccg cgt ggc tgg gaa cag ctg gca gac gtg gtt tcc         48
Arg His Arg Gln Pro Arg Gly Trp Glu Gln Leu Ala Asp Val Val Ser           
1               5                   10                  15                

ctg acc tgt ccg gtt gct gcg ggc gaa tgt gcg ggt ccg gca gat tcc         96
Leu Thr Cys Pro Val Ala Ala Gly Glu Cys Ala Gly Pro Ala Asp Ser           
            20                  25                  30                    

ggt gat gca ctg ctg gaa cgt aac tat ccg acc ggc gcc gaa ttt ctg        144
Gly Asp Ala Leu Leu Glu Arg Asn Tyr Pro Thr Gly Ala Glu Phe Leu           
        35                  40                  45                        

ggt gat ggc ggt gca att tca ttc tcg acc cgc ggc acg cag aat tgg        192
Gly Asp Gly Gly Ala Ile Ser Phe Ser Thr Arg Gly Thr Gln Asn Trp           
    50                  55                  60                            

acg gtg gaa cgt ctg ctg caa gcc cat cgc caa ctg gaa gaa ggc ggt        240
Thr Val Glu Arg Leu Leu Gln Ala His Arg Gln Leu Glu Glu Gly Gly           
65                  70                  75                  80            

tat gtt ttt gtc ggc tat cat ggc acc ttt ctg gaa gcc gca cag tca        288
Tyr Val Phe Val Gly Tyr His Gly Thr Phe Leu Glu Ala Ala Gln Ser           
                85                  90                  95                

atc gtg ttt ggc ggt gtt cgt gcg cgc tcg cag gat ctg gat gct att        336
Ile Val Phe Gly Gly Val Arg Ala Arg Ser Gln Asp Leu Asp Ala Ile           
            100                 105                 110                   

tgg gcg ggc ttc tac atc gcc ggt gat ccg gcg ctg gcg tat ggc tac        384
Trp Ala Gly Phe Tyr Ile Ala Gly Asp Pro Ala Leu Ala Tyr Gly Tyr           
        115                 120                 125                       

gca cag gat caa gaa ccg gac gct gcg ggc cgt att cgc aac ggt gcg        432
Ala Gln Asp Gln Glu Pro Asp Ala Ala Gly Arg Ile Arg Asn Gly Ala           
    130                 135                 140                           

ctg ctg cgt gtg tat gtt ccg cgc agc tct ctg ccg ggc ttt tat gca        480
Leu Leu Arg Val Tyr Val Pro Arg Ser Ser Leu Pro Gly Phe Tyr Ala           
145                 150                 155                 160           

acc ggt ctg acg ctg gcc gca ccg gaa gct gcg ggc gaa gtg gaa cgt        528
Thr Gly Leu Thr Leu Ala Ala Pro Glu Ala Ala Gly Glu Val Glu Arg           
                165                 170                 175               

ctg att ggt cac ccg ctg ccg ctg cgc ctg gat gct atc acc ggt ccg        576
Leu Ile Gly His Pro Leu Pro Leu Arg Leu Asp Ala Ile Thr Gly Pro           
            180                 185                 190                   

gaa gaa gcg ggc ggt cgt ctg gaa acg att ctg ggt tgg ccg ctg gct        624
Glu Glu Ala Gly Gly Arg Leu Glu Thr Ile Leu Gly Trp Pro Leu Ala           
        195                 200                 205                       

gaa cgc acc gtc gtg att ccg agc gcg atc ccg acc gat ccg cgt aat        672
Glu Arg Thr Val Val Ile Pro Ser Ala Ile Pro Thr Asp Pro Arg Asn           
    210                 215                 220                           

gtt ggc ggt gat ctg gac ccg agt tcc atc ccg gac tct gaa gcc gca        720
Val Gly Gly Asp Leu Asp Pro Ser Ser Ile Pro Asp Ser Glu Ala Ala           
225                 230                 235                 240           

atc tca gcc ctg ccg gat tat gcc agc caa ccg ggt aaa ccg ccg aaa        768
Ile Ser Ala Leu Pro Asp Tyr Ala Ser Gln Pro Gly Lys Pro Pro Lys           
                245                 250                 255               

gac gaa ctg                                                            777
Asp Glu Leu                                                               
                                                                          


<210>  6
<211>  259
<212>  PRT
<213>  Pseudomonas aeruginosa

<400>  6

Arg His Arg Gln Pro Arg Gly Trp Glu Gln Leu Ala Asp Val Val Ser 
1               5                   10                  15      


Leu Thr Cys Pro Val Ala Ala Gly Glu Cys Ala Gly Pro Ala Asp Ser 
            20                  25                  30          


Gly Asp Ala Leu Leu Glu Arg Asn Tyr Pro Thr Gly Ala Glu Phe Leu 
        35                  40                  45              


Gly Asp Gly Gly Ala Ile Ser Phe Ser Thr Arg Gly Thr Gln Asn Trp 
    50                  55                  60                  


Thr Val Glu Arg Leu Leu Gln Ala His Arg Gln Leu Glu Glu Gly Gly 
65                  70                  75                  80  


Tyr Val Phe Val Gly Tyr His Gly Thr Phe Leu Glu Ala Ala Gln Ser 
                85                  90                  95      


Ile Val Phe Gly Gly Val Arg Ala Arg Ser Gln Asp Leu Asp Ala Ile 
            100                 105                 110         


Trp Ala Gly Phe Tyr Ile Ala Gly Asp Pro Ala Leu Ala Tyr Gly Tyr 
        115                 120                 125             


Ala Gln Asp Gln Glu Pro Asp Ala Ala Gly Arg Ile Arg Asn Gly Ala 
    130                 135                 140                 


Leu Leu Arg Val Tyr Val Pro Arg Ser Ser Leu Pro Gly Phe Tyr Ala 
145                 150                 155                 160 


Thr Gly Leu Thr Leu Ala Ala Pro Glu Ala Ala Gly Glu Val Glu Arg 
                165                 170                 175     


Leu Ile Gly His Pro Leu Pro Leu Arg Leu Asp Ala Ile Thr Gly Pro 
            180                 185                 190         


Glu Glu Ala Gly Gly Arg Leu Glu Thr Ile Leu Gly Trp Pro Leu Ala 
        195                 200                 205             


Glu Arg Thr Val Val Ile Pro Ser Ala Ile Pro Thr Asp Pro Arg Asn 
    210                 215                 220                 


Val Gly Gly Asp Leu Asp Pro Ser Ser Ile Pro Asp Ser Glu Ala Ala 
225                 230                 235                 240 


Ile Ser Ala Leu Pro Asp Tyr Ala Ser Gln Pro Gly Lys Pro Pro Lys 
                245                 250                 255     


Asp Glu Leu 
            


<210>  7
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of connector


<220>
<221>  CDS
<222>  (1)..(27)

<400>  7
gct agc ggc ggc ccg gaa ggc ggc agc                                     27
Ala Ser Gly Gly Pro Glu Gly Gly Ser                                       
1               5                                                         


<210>  8
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  8

Ala Ser Gly Gly Pro Glu Gly Gly Ser 
1               5                   


<210>  9
<211>  357
<212>  DNA
<213>  Homo sapiens


<220>
<221>  CDS
<222>  (1)..(357)

<400>  9
ggt cga ggt gca ctg gtg gag tct ggc cca gga ctg gtg aag cct tcg         48
Gly Arg Gly Ala Leu Val Glu Ser Gly Pro Gly Leu Val Lys Pro Ser           
1               5                   10                  15                

gag acc ctg ttc ctc acc tgc act gtc tct ggt ggc tcc atc agt tct         96
Glu Thr Leu Phe Leu Thr Cys Thr Val Ser Gly Gly Ser Ile Ser Ser           
            20                  25                  30                    

tac tac tgg agc tgg atc cgg cag ccc gca ggg aag gga ctg gag tgg        144
Tyr Tyr Trp Ser Trp Ile Arg Gln Pro Ala Gly Lys Gly Leu Glu Trp           
        35                  40                  45                        

att gcg tat atc tat tac agt ggg agc acc aac tac aac ccc tcc ctc        192
Ile Ala Tyr Ile Tyr Tyr Ser Gly Ser Thr Asn Tyr Asn Pro Ser Leu           
    50                  55                  60                            

aag agt cga gtc acc ata tca gta gac acg tcc aac aac cag ttc tcc        240
Lys Ser Arg Val Thr Ile Ser Val Asp Thr Ser Asn Asn Gln Phe Ser           
65                  70                  75                  80            

ctg aaa ctg agc tct gtg acc gct gcg gac acg gcc gtg tat tat tgt        288
Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys           
                85                  90                  95                

gcg aag tta aca gca gcc ggg ggc cac ttc gac ccc tgg ggc cag ggc        336
Ala Lys Leu Thr Ala Ala Gly Gly His Phe Asp Pro Trp Gly Gln Gly           
            100                 105                 110                   

acc ctg gtc acc gtc tcc tca                                            357
Thr Leu Val Thr Val Ser Ser                                               
        115                                                               


<210>  10
<211>  119
<212>  PRT
<213>  Homo sapiens

<400>  10

Gly Arg Gly Ala Leu Val Glu Ser Gly Pro Gly Leu Val Lys Pro Ser 
1               5                   10                  15      


Glu Thr Leu Phe Leu Thr Cys Thr Val Ser Gly Gly Ser Ile Ser Ser 
            20                  25                  30          


Tyr Tyr Trp Ser Trp Ile Arg Gln Pro Ala Gly Lys Gly Leu Glu Trp 
        35                  40                  45              


Ile Ala Tyr Ile Tyr Tyr Ser Gly Ser Thr Asn Tyr Asn Pro Ser Leu 
    50                  55                  60                  


Lys Ser Arg Val Thr Ile Ser Val Asp Thr Ser Asn Asn Gln Phe Ser 
65                  70                  75                  80  


Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Leu Thr Ala Ala Gly Gly His Phe Asp Pro Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  11
<211>  324
<212>  DNA
<213>  Homo sapiens


<220>
<221>  CDS
<222>  (1)..(324)

<400>  11
gac atc gtg atg acc cag tct cca tcc tcc ctg tct gca tct ata ggg         48
Asp Ile Val Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Ile Gly           
1               5                   10                  15                

gac aga gtc acc atc act tgt cgg gca agt cag ggc att agc aat ttt         96
Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Ser Asn Phe           
            20                  25                  30                    

tta gcc tgg ttt cag cag aaa cca ggg aaa gcc cct aag tcc ctg atc        144
Leu Ala Trp Phe Gln Gln Lys Pro Gly Lys Ala Pro Lys Ser Leu Ile           
        35                  40                  45                        

tat gct gca tcc agt ttg caa agt ggg gtc cca tca aag ttc agc ggc        192
Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Lys Phe Ser Gly           
    50                  55                  60                            

agt gga tct ggg aca gat ttc act ctc gcc atc agc agc ctg cag cct        240
Ser Gly Ser Gly Thr Asp Phe Thr Leu Ala Ile Ser Ser Leu Gln Pro           
65                  70                  75                  80            

gaa gat ttt gca act tat tac tgt ctc caa gat tcc gat tat cct ctc        288
Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln Asp Ser Asp Tyr Pro Leu           
                85                  90                  95                

act ttc ggc gga ggg acc aag ctg gag atc aaa cgt                        324
Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg                           
            100                 105                                       


<210>  12
<211>  108
<212>  PRT
<213>  Homo sapiens

<400>  12

Asp Ile Val Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Ile Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Ser Asn Phe 
            20                  25                  30          


Leu Ala Trp Phe Gln Gln Lys Pro Gly Lys Ala Pro Lys Ser Leu Ile 
        35                  40                  45              


Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Lys Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Ala Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln Asp Ser Asp Tyr Pro Leu 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
            100                 105             


<210>  13
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of linker


<220>
<221>  CDS
<222>  (1)..(45)

<400>  13
ggt gga ggc ggt tca ggc gga ggt ggc agc ggc ggt ggc gga tcg             45
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser               
1               5                   10                  15                


<210>  14
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  14

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  15
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sequence of CDR1 of VH

<400>  15

Ser Tyr Tyr Trp Ser 
1               5   


<210>  16
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sequence of CDR2 of VH

<400>  16

Tyr Ile Tyr Tyr Ser Gly Ser Thr Asn Tyr Asn Pro Ser Leu Lys Ser 
1               5                   10                  15      


<210>  17
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sequence of CDR3 of VH

<400>  17

Leu Thr Ala Ala Gly Gly His Phe Asp Pro 
1               5                   10  


<210>  18
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sequence of CDR1  of VL

<400>  18

Arg Ala Ser Gln Gly Ile Ser Asn Phe Leu Ala 
1               5                   10      


<210>  19
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sequence of CDR2 of VL

<400>  19

Ala Ala Ser Ser Leu Gln Ser 
1               5           


<210>  20
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sequence of CDR3 of VL

<400>  20

Leu Gln Asp Ser Asp Tyr Pro Leu Thr 
1               5                   


<210>  21
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sequence of furin cleavage site of PEA

<400>  21

Arg His Arg Gln Pro Arg Gly Trp Glu Gln Leu 
1               5                   10      


<210>  22
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sequence of domain Ib of PEA

<400>  22

Ala Asp Val Val Ser Leu Thr Cys Pro Val Ala Ala Gly Glu Cys Ala 
1               5                   10                  15      


Gly Pro Ala Asp Ser Gly Asp Ala Leu Leu Glu Arg Asn Tyr Pro Thr 
            20                  25                  30          


Gly Ala Glu Phe Leu Gly Asp Gly 
        35                  40  


<210>  23
<211>  204
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sequence domain III of PEA

<400>  23

Gly Ala Ile Ser Phe Ser Thr Arg Gly Thr Gln Asn Trp Thr Val Glu 
1               5                   10                  15      


Arg Leu Leu Gln Ala His Arg Gln Leu Glu Glu Gly Gly Tyr Val Phe 
            20                  25                  30          


Val Gly Tyr His Gly Thr Phe Leu Glu Ala Ala Gln Ser Ile Val Phe 
        35                  40                  45              


Gly Gly Val Arg Ala Arg Ser Gln Asp Leu Asp Ala Ile Trp Ala Gly 
    50                  55                  60                  


Phe Tyr Ile Ala Gly Asp Pro Ala Leu Ala Tyr Gly Tyr Ala Gln Asp 
65                  70                  75                  80  


Gln Glu Pro Asp Ala Ala Gly Arg Ile Arg Asn Gly Ala Leu Leu Arg 
                85                  90                  95      


Val Tyr Val Pro Arg Ser Ser Leu Pro Gly Phe Tyr Ala Thr Gly Leu 
            100                 105                 110         


Thr Leu Ala Ala Pro Glu Ala Ala Gly Glu Val Glu Arg Leu Ile Gly 
        115                 120                 125             


His Pro Leu Pro Leu Arg Leu Asp Ala Ile Thr Gly Pro Glu Glu Ala 
    130                 135                 140                 


Gly Gly Arg Leu Glu Thr Ile Leu Gly Trp Pro Leu Ala Glu Arg Thr 
145                 150                 155                 160 


Val Val Ile Pro Ser Ala Ile Pro Thr Asp Pro Arg Asn Val Gly Gly 
                165                 170                 175     


Asp Leu Asp Pro Ser Ser Ile Pro Asp Ser Glu Ala Ala Ile Ser Ala 
            180                 185                 190         


Leu Pro Asp Tyr Ala Ser Gln Pro Gly Lys Pro Pro 
        195                 200                 


<210>  24
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sequence of carboxy terminal of PEA

<400>  24

Lys Asp Glu Leu 
1               


<210>  25
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  25

Ser Glu Gln Asp Gln Asp Pro Gln Arg Val 
1               5                   10  


