                         SEQUENCE LISTING

<110>  INSERM, Universit Paris 5 Ren Descartes, APHP, Imagine
 
<120>  Recombinant lentiviral vector for stem cell-based gene therapy of
        Sickle cell disorder

<130>  1H507650 Cas 0072

<160>  94    

<170>  PatentIn version 3.5

<210>  1
<211>  830
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Beta AS1 (T87Q) (not modified)

<400>  1
ttagtgatac ttgtgggcca gggcattagc cacaccagcc accactttct gataggcagc       60

ctgcactggt ggggtgaatt ctttgccaaa gtgatgggcc agcacacaga ccagcacgtt      120

gcccaggagc tgtgggagga agataagagg tatgaacatg attagcaaaa gggcctagct      180

tggactcaga ataatccagc cttatcccaa ccataaaata aaagcagaat ggtagctgga      240

ttgtagctgc tattagcaat atgaaacctc ttacatcagt tacaatttat atgcagaaat      300

accctgttac ttctcccctt cctatgacat gaacttaacc atagaaaaga aggggaaaga      360

aaacatcaag ggtcccatag actcaccctg aagttctcag gatccacgtg cagcttgtca      420

cagtgcagct cactcagctg ggcaaaggtg cccttgaggt tgtccaggtg agccaggcca      480

tcactaaagg caccgagcac tttcttgcca tgagccttca ccttagggtt gcccataaca      540

gcatcaggag tggacagatc cccaaaggac tcaaagaacc tctgggtcca agggtagacc      600

accagcagcc taagggtggg aaaatagacc aataggcaga gagagtcagt gcctatcaga      660

aacccaagag tcttctctgt ctccacatgc ccagtttcta ttggtctcct taaacctgtc      720

ttgtaacctt gataccaacc tgcccagggc ctcaccacca acttcatcca cgttcacctt      780

gccccacagg gcagtaacgg cagacttctc ctcaggagtc aggtgcacca                 830


<210>  2
<211>  831
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Beta AS3 (not modified)

<400>  2
ttagtgatac ttgtgggcca gggcattagc cacaccagcc accactttct gataggcagc       60

ctgcactggt ggggtgaatt ctttgccaaa gtgatgggcc agcacacaga ccagcacgtt      120

gcccaggagc tgtgggagga agataagagg tatgaacatg attagcaaaa gggcctagct      180

tggactcaga ataatccagc cttatcccaa ccataaaata aaagcagaat ggtagctgga      240

ttgtagctgc tattagcaat atgaaacctc ttacatcagt tacaatttat atgcagaaat      300

accctgttac ttctcccctt cctatgacat gaacttaacc atagaaaaga aggggaaaga      360

aaacatcaag ggtcccatag actcaccctg aagttctcag gatccacgtg cagcttgtca      420

cagtgcagct cactcagctg ggcaaaggtg cccttgaggt tgtccaggtg agccaggcca      480

tcactaaagg caccgagcac tttcttgcca tgagccttca ccttagggtt gcccataaca      540

gcatcaggag tggacagatc cccaaaggac tcaaagaacc tctgggtcca agggtagacc      600

accagcagcc taagggtggg aaaatagacc aataggcaga gagagtcagt gcctatcaga      660

aacccaagag tcttctctgt ctccacatgc ccagtttcta ttggtctcct taaacctgtc      720

ttgtaacctt gataccaacc tgcccagggc ctcaccacca acggcatcca cgttcacctt      780

gtcccacagg gcagtaacgg cagacttctc ctcaggagtc aggtgcacca t               831


<210>  3
<211>  848
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gamma-beta hybrid

<400>  3
tcagtggtat ctggaggaca gggcactggc cactgcagtc accatcttct gccaggaagc       60

ctgcacctca ggggtgaatt ctttgccgaa atggattgcc aaaacggtca ccagcacatt      120

tcccaggagc tgtgggagga agataagagg tatgaacatg attagcaaaa gggcctagct      180

tggactcaga ataatccagc cttatcccaa ccataaaata aaagcagaat ggtagctgga      240

ttgtagctgc tattagcaat atgaaacctc ttacatcagt tacaatttat atgcagaaat      300

accctgttac ttctcccctt cctatgacat gaacttaacc atagaaaaga aggggaaaga      360

aaacatcaag ggtcccatag actcacgggt cccatagact caccttgaag ttctcaggat      420

ccacatgcag cttgtcacag tgcagttcac tcagctgggc aaaggtgccc ttgagatcat      480

ccaggtgctt tgtggcatct cccaaggaag tcagcacctt cttgccatgt gccttgactt      540

tggggttgcc catgatggca gaggcagagg acaggttgcc aaagctgtca aagaacctct      600

gggtccatgg gtagacaacc aggagcctaa gggtgggaaa atagaccaat aggcagagag      660

agtcagtgcc tatcagaaac ccaagagtct tctctgtctc cacatgccca gtttctattg      720

gtctccttaa acctgtcttg taaccttgat accaaccttc ccagggtttc tcctccagca      780

tcttccacat tcaccttgcc ccacaggctt gtgatagtag ccttgtcctc ctctgtgaaa      840

tgacccat                                                               848


<210>  4
<211>  848
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gamma-beta hybrid AS2 (G16D and D22A)

<400>  4
tcagtggtat ctggaggaca gggcactggc cactgcagtc accatcttct gccaggaagc       60

ctgcacctca ggggtgaatt ctttgccgaa atggattgcc aaaacggtca ccagcacatt      120

tcccaggagc tgtgggagga agataagagg tatgaacatg attagcaaaa gggcctagct      180

tggactcaga ataatccagc cttatcccaa ccataaaata aaagcagaat ggtagctgga      240

ttgtagctgc tattagcaat atgaaacctc ttacatcagt tacaatttat atgcagaaat      300

accctgttac ttctcccctt cctatgacat gaacttaacc atagaaaaga aggggaaaga      360

aaacatcaag ggtcccatag actcacgggt cccatagact caccttgaag ttctcaggat      420

ccacatgcag cttgtcacag tgcagttcac tcagctgggc aaaggtgccc ttgagatcat      480

ccaggtgctt tgtggcatct cccaaggaag tcagcacctt cttgccatgt gccttgactt      540

tggggttgcc catgatggca gaggcagagg acaggttgcc aaagctgtca aagaacctct      600

gggtccatgg gtagacaacc aggagcctaa gggtgggaaa atagaccaat aggcagagag      660

agtcagtgcc tatcagaaac ccaagagtct tctctgtctc cacatgccca gtttctattg      720

gtctccttaa acctgtcttg taaccttgat accaaccttc ccagggtttc tcctccagcg      780

gcttccacat tcaccttgtc ccacaggctt gtgatagtag ccttgtcctc ctctgtgaaa      840

tgacccat                                                               848


<210>  5
<211>  831
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Delta-beta hybrid

<400>  5
tcaatggtac ttgtgagcca gggcattagc cacaccagcc accaccttct gataggcagc       60

ctgcatttgt ggggtgaatt ccttgccaaa gttgcgggcc agcacacaca ccagcacatt      120

gcccaagagc tgtgggagga agataagagg tatgaacatg attagcaaaa gggcctagct      180

tggactcaga ataatccagc cttatcccaa ccataaaata aaagcagaat ggtagctgga      240

ttgtagctgc tattagcaat atgaaacctc ttacatcagt tacaatttat atgcagaaat      300

accctgttac ttctcccctt cctatgacat gaacttaacc atagaaaaga aggggaaaga      360

aaacatcaag ggtcccatag actcaccctg aagttctcag gatccacgtg cagcttgtca      420

cagtgcagct cactcagctg agaaaaagtg cccttgaggt tgtccaggtg agccaggcca      480

tcactaaagg cacctagcac cttcttgcca tgagccttca ccttagggtt gcccataaca      540

gcatcaggag aggacagatc cccaaaggac tcaaagaacc tctgggtcca agggtagacc      600

accagtaatc taagggtggg aaaatagacc aataggcaga gagagtcagt gcctatcaga      660

aacccaagag tcttctctgt ctccacatgc ccagtttcta ttggtctcct taaacctgtc      720

ttgtaacctt gataccaacc tgcccagggc ctcaccacca actgcatcca cgttcacttt      780

gccccacagg gcattgacag cagtcttctc ctcaggagtc agatgcacca t               831


<210>  6
<211>  848
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Delta-beta hybrid AS1 (G16D)

<400>  6
tcaatggtac ttgtgagcca gggcattagc cacaccagcc accaccttct gataggcagc       60

ctgcatttgt ggggtgaatt ccttgccaaa gttgcgggcc agcacacaca ccagcacatt      120

gcccaagagc tgtgggagga agataagagg tatgaacatg attagcaaaa gggcctagct      180

tggactcaga ataatccagc cttatcccaa ccataaaata aaagcagaat ggtagctgga      240

ttgtagctgc tattagcaat atgaaacctc ttacatcagt tacaatttat atgcagaaat      300

accctgttac ttctcccctt cctatgacat gaacttaacc atagaaaaga aggggaaaga      360

aaacatcaag ggtcccatag actcacgggt cccatagact caccctgaag ttctcaggat      420

ccacgtgcag cttgtcacag tgcagctcac tcagctgaga aaaagtgccc ttgaggttgt      480

ccaggtgagc caggccatca ctaaaggcac ctagcacctt cttgccatga gccttcacct      540

tagggttgcc cataacagca tcaggagagg acagatcccc aaaggactca aagaacctct      600

gggtccaagg gtagaccacc agtaatctaa gggtgggaaa atagaccaat aggcagagag      660

agtcagtgcc tatcagaaac ccaagagtct tctctgtctc cacatgccca gtttctattg      720

gtctccttaa acctgtcttg taaccttgat accaacctgc ccagggcctc accaccaact      780

gcatccacgt tcactttgtc ccacagggca ttgacagcag tcttctcctc aggagtcaga      840

tgcaccat                                                               848


<210>  7
<211>  831
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Beta AS1 (T87Q) (modified to avoid targeting by gRNA D)

<400>  7
ttagtgatac ttgtgggcca gggcattagc cacaccagcc accactttct gataggcagc       60

ctgcactggt ggggtgaatt ctttgccaaa gtgatgggcc agcacacaga ccagcacgtt      120

gcccaggagc tgtgggagga agataagagg tatgaacatg attagcaaaa gggcctagct      180

tggactcaga ataatccagc cttatcccaa ccataaaata aaagcagaat ggtagctgga      240

ttgtagctgc tattagcaat atgaaacctc ttacatcagt tacaatttat atgcagaaat      300

accctgttac ttctcccctt cctatgacat gaacttaacc atagaaaaga aggggaaaga      360

aaacatcaag ggtcccatag actcaccctg aagttctcag gatccacgtg cagcttgtca      420

cagtgcagct cactcagctg ggcaaaggtg cccttgaggt tgtccaggtg agccaggcca      480

tcactaaagg caccgagcac tttcttgcca tgagccttca ccttagggtt gcccataaca      540

gcatcaggag tggacagatc cccaaaggac tcaaagaacc tctgggtcca agggtagacc      600

accagcagcc taagggtggg aaaatagacc aataggcaga gagagtcagt gcctatcaga      660

aacccaagag tcttctctgt ctccacatgc ccagtttcta ttggtctcct taaacctgtc      720

ttgtaacctt gataccaacc tgcccagggc ctcaccacca acttcatcca cgttcacctt      780

gccccagaga gcggtcacag cggacttctc ctcaggagtc aggtgcacca t               831


<210>  8
<211>  831
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Beta AS3 (modified to avoid targeting by gRNA D)

<400>  8
ttagtgatac ttgtgggcca gggcattagc cacaccagcc accactttct gataggcagc       60

ctgcactggt ggggtgaatt ctttgccaaa gtgatgggcc agcacacaga ccagcacgtt      120

gcccaggagc tgtgggagga agataagagg tatgaacatg attagcaaaa gggcctagct      180

tggactcaga ataatccagc cttatcccaa ccataaaata aaagcagaat ggtagctgga      240

ttgtagctgc tattagcaat atgaaacctc ttacatcagt tacaatttat atgcagaaat      300

accctgttac ttctcccctt cctatgacat gaacttaacc atagaaaaga aggggaaaga      360

aaacatcaag ggtcccatag actcaccctg aagttctcag gatccacgtg cagcttgtca      420

cagtgcagct cactcagctg ggcaaaggtg cccttgaggt tgtccaggtg agccaggcca      480

tcactaaagg caccgagcac tttcttgcca tgagccttca ccttagggtt gcccataaca      540

gcatcaggag tggacagatc cccaaaggac tcaaagaacc tctgggtcca agggtagacc      600

accagcagcc taagggtggg aaaatagacc aataggcaga gagagtcagt gcctatcaga      660

aacccaagag tcttctctgt ctccacatgc ccagtttcta ttggtctcct taaacctgtc      720

ttgtaacctt gataccaacc tgcccagggc ctcaccacca acggcatcca cgttcacctt      780

gtcccagaga gcggtcacag cggacttctc ctcaggagtc aggtgcacca t               831


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer HBBex1 F

<400>  9
cagcatcagg agtggacaga                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer HBBex1 R

<400>  10
agtcagggca gagccatcta                                                   20


<210>  11
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer HBB F

<400>  11
gcaaggtgaa cgtggatgaa gt                                                22


<210>  12
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer HBB R

<400>  12
taacagcatc aggagtggac aga                                               23


<210>  13
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer HBA1 F

<400>  13
cggtcaactt caagctccta a                                                 21


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer HBA1 R

<400>  14
acagaagcca ggaacttgtc                                                   20


<210>  15
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligo FOR-Opt_gRNA B

<400>  15
caccgtaacg gcagacttct cctc                                              24


<210>  16
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligo REV-Opt_gRNA B

<400>  16
aaacgaggag aagtctgccg ttac                                              24


<210>  17
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligo FOR-Opt_gRNA D

<400>  17
caccgtctgc cgttactgcc ctgt                                              24


<210>  18
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligo REV-Opt_gRNA D

<400>  18
aaacacaggg cagtaacggc agac                                              24


<210>  19
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligo FOR-Opt_gRNA E

<400>  19
caccgaaggt gaacgtggat gaagt                                             25


<210>  20
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligo REV-Opt_gRNA E

<400>  20
aaacacttca tccacgttca ccttc                                             25


<210>  21
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer HBB-AS3 F

<400>  21
aagggcacct ttgcccag                                                     18


<210>  22
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer HBB-AS3 R

<400>  22
gccaccactt tctgataggc ag                                                22


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA A spacer

<400>  23
cttgccccac agggcagtaa                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA B spacer

<400>  24
gtaacggcag acttctcctc                                                   20


<210>  25
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA D spacer

<400>  25
tctgccgtta ctgccctgt                                                    19


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA E spacer

<400>  26
aaggtgaacg tggatgaagt                                                   20


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA F spacer

<400>  27
gtgtggcaaa ggtgcccttg                                                   20


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA G spacer

<400>  28
acagtgcagc tcactcagtg                                                   20


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA H spacer

<400>  29
tctgccgtta ctgccctgtg                                                   20


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA I spacer

<400>  30
cgttactgcc ctgtggggca                                                   20


<210>  31
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA J spacer

<400>  31
gtctgccgtt actgccctgt                                                   20


<210>  32
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA K spacer

<400>  32
cagctcactc agtgtggcaa                                                   20


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA L spacer

<400>  33
tcccaccctt aggctgctgg                                                   20


<210>  34
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA M spacer

<400>  34
ggctgctggt ggtctaccct                                                   20


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA N spacer

<400>  35
ccccacaggg cagtaacggc                                                   20


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA O spacer

<400>  36
taacggcaga cttctccaca                                                   20


<210>  37
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligo FOR-gRNA A

<400>  37
acaccgcttg ccccacaggg cagtaag                                           27


<210>  38
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligo REV-gRNA A

<400>  38
aaaacttact gccctgtggg gcaagcg                                           27


<210>  39
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligo FOR-gRNA B

<400>  39
acaccgtaac ggcagacttc tcctcg                                            26


<210>  40
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligo REV-gRNA B

<400>  40
aaaacgagga gaagtctgcc gttacg                                            26


<210>  41
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligo FOR-gRNA D

<400>  41
acaccgtctg ccgttactgc cctgtg                                            26


<210>  42
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligo REV-gRNA D

<400>  42
aaaacacagg gcagtaacgg cagacg                                            26


<210>  43
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligo FOR-gRNA E

<400>  43
acaccgaagg tgaacgtgga tgaagtg                                           27


<210>  44
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligo REV-gRNA E

<400>  44
aaaacacttc atccacgttc accttcg                                           27


<210>  45
<211>  10195
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LV.GLOBE.betaAS3-GLOBIN

<400>  45
ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc atgtccaaca       60

ttaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca      120

ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct      180

ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta      240

acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac      300

ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt      360

aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag      420

tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat      480

gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat      540

gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc      600

ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt      660

ttagtgaacc ggggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac      720

tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg      780

cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga      840

aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct      900

ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact      960

ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag     1020

cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg     1080

gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt     1140

cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct     1200

acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac     1260

cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat     1320

agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac     1380

ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa     1440

aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa     1500

aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta     1560

tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc     1620

agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag     1680

tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc     1740

aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt     1800

ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg     1860

agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc     1920

aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt     1980

ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag     2040

gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc     2100

agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc     2160

ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga     2220

acggatctcg acggtatcgg ttaactttta aaagaaaagg ggggattggg gggtacagtg     2280

caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac     2340

aaattacaaa attcaaaatt ttatcggtac gtaccatgag gacagctaaa acaataagta     2400

atgtaaaata cagcatagca aaactttaac ctccaaatca agcctctact tgaatccttt     2460

tctgagggat gaataaggca taggcatcag gggctgttgc caatgtgcat tagctgtttg     2520

cagcctcacc ttctttcatg gagtttaaga tatagtgtat tttcccaagg tttgaactag     2580

ctcttcattt ctttatgttt taaatgcact gacctcccac attccctttt tagtaaaata     2640

ttcagaaata atttaaatac atcattgcaa tgaaaataaa tgttttttat taggcagaat     2700

ccagatgctc aaggcccttc ataatatccc ccagtttagt agttggactt agggaacaaa     2760

ggaaccttta atagaaattg gacagcaaga aagcgagctt agtgatactt gtgggccagg     2820

gcattagcca caccagccac cactttctga taggcagcct gcactggtgg ggtgaattct     2880

ttgccaaagt gatgggccag cacacagacc agcacgttgc ccaggagctg tgggaggaag     2940

ataagaggta tgaacatgat tagcaaaagg gcctagcttg gactcagaat aatccagcct     3000

tatcccaacc ataaaataaa agcagaatgg tagctggatt gtagctgcta ttagcaatat     3060

gaaacctctt acatcagtta caatttatat gcagaaatac cctgttactt ctccccttcc     3120

tatgacatga acttaaccat agaaaagaag gggaaagaaa acatcaaggg tcccatagac     3180

tcaccctgaa gttctcagga tccacgtgca gcttgtcaca gtgcagctca ctcagctggg     3240

caaaggtgcc cttgaggttg tccaggtgag ccaggccatc actaaaggca ccgagcactt     3300

tcttgccatg agccttcacc ttagggttgc ccataacagc atcaggagtg gacagatccc     3360

caaaggactc aaagaacctc tgggtccaag ggtagaccac cagcagccta agggtgggaa     3420

aatagaccaa taggcagaga gagtcagtgc ctatcagaaa cccaagagtc ttctctgtct     3480

ccacatgccc agtttctatt ggtctcctta aacctgtctt gtaaccttga taccaacctg     3540

cccagggcct caccaccaac ggcatccacg ttcaccttgt cccagagagc ggtcacagcg     3600

gacttctcct caggagtcag gtgcaccatg gtgtctgttt gaggttgcta gtgaacacag     3660

ttgtgtcaga agcaaatgta agcaatagat ggctctgccc tgacttttat gcccagccct     3720

ggctcctgcc ctccctgctc ctgggagtag attggccaac cctagggtgt ggctccacag     3780

ggtgaggtct aagtgatgac agccgtacct gtccttggct cttctggcac tggcttagga     3840

gttggacttc aaaccctcag ccctccctct aagatatatc tcttggcccc ataccatcag     3900

tacaaattgc tactaaaaac atcctccttt gcaagtgtat ttacacggta tcgataagct     3960

tgatatcgaa ttcctgcagc ccccttttgc cacctagctg tccaggggtg ccttaaaatg     4020

gcaaacaagg tttgttttct tttcctgttt tcatgccttc ctcttccata tccttgtttc     4080

atattaatac atgtgtatag atcctaaaaa tctatacaca tgtattaata aagcctgatt     4140

ctgccgcttc taggtataga ggccacctgc aagataaata tttgattcac aataactaat     4200

cattctatgg caattgataa caacaaatat atatatatat atatatacgt atatgtgtat     4260

atatatatat atatattcag gaaataatat attctagaat atgtcacatt ctgtctcagg     4320

catccatttt ctttatgatg ccgtttgagg tggagtttta gtcaggtggt cagcttctcc     4380

ttttttttgc catctgccct gtaagcatcc tgctggggac ccagatagga gtcatcactc     4440

taggctgaga acatctgggc acacacccta agcctcagca tgactcatca tgactcagca     4500

ttgctgtgct tgagccagaa ggtttgctta gaaggttaca cagaaccaga aggcgggggt     4560

ggggcactga ccccgacagg ggcctggcca gaactgctca tgcttggact atgggaggtc     4620

actaatggag acacacagaa atgtaacagg aactaaggaa aaactgaagc ttatttaatc     4680

agagatgagg atgctggaag ggatagaggg agctgagctt gtaaaaagta tagtaatcat     4740

tcagcaaatg gttttgaagc acctgctgga tgctaaacac tattttcagt gcttgaatca     4800

taaataagaa taaaacatgt atcttattcc ccacaagagt ccaagtaaaa aataacagtt     4860

aattataatg tgctctgtcc cccaggctgg agtgcagtgg cacgatctca gctcactgca     4920

acctccgcct cccgggttca agcaattctc ctgcctcagc caccctaata gctgggatta     4980

caggtgcaca ccaccatgcc aggctaattt ttgtactttt tgtagaggca gggtatcacc     5040

atgttgtcca agatggtctt gaactcctga gctccaagca gtccacccac ctcagcctcc     5100

caaagtgctg ggattacagg tgtgagacac catgcccaga ttttccatat ttaatagagg     5160

tatttatggg atgggggaaa agaatgtttc tctcactgtg gattatttta gagagtggag     5220

aatggtcaag atttttttaa aaattaagaa aacataagtt ggaccttgag aaatgaaaat     5280

ttattttttt gttggaggat acccattctc tatctcccat cagggcaagc tgtaaggaac     5340

tggctaagac acagtgagac agagtgactt agtcttagag gccccactgg tacgacggtc     5400

accaagcttt cattaaaaaa agtctaacca gctgcattcg actttgactg cagcagctgg     5460

ttagaaggtt ctactggagg agggtcccag cccattgcta aattaacatc aggctctgag     5520

actggcagta tatctctaac agtggttgat gctatcttct ggaacttgcc tgctacattg     5580

agaccactga cccatacata ggaagcccat agctctgtcc tgaactgtta ggccactggt     5640

ccagagagtg tgcatctcct ttgatcctca taataaccct atgagataga cacaattatt     5700

actcttactt tatagatgat gatcctgaaa acataggagt caaggcactt gcccctagct     5760

gggggtatag gggagcagtc ccatgtagta gtagaatgaa aaatgctgct atgctgtgcc     5820

tcccccacct ttcccatgtc tgccctctac tcatggtcta tctctcctgg ctcctgggag     5880

tcatggactc cacccagcac caccaacctg acctaaccac ctatctgagc ctgccagcct     5940

ataacccatc tgggccctga tagctggtgg ccagccctga ccccacccca ccctccctgg     6000

aacctctgat agacacatct ggcacaccag ctcgcaaagt caccgtgagg gtcttgtgtt     6060

tgctgagtca aaattccttg aaatccaagt ccttagagac tcctgctccc aaatttacag     6120

tcatagactt cttcatggct gtctccttta tccacagaat gattcctttg cttcattgcc     6180

ccatccatct gatcctcctc atcagtgcag cacagggccc atgagcagta gctgcagagt     6240

ctcacatagg tctggcactg cctctgacat gtccgacctt aggcaaatgc ttgactcttc     6300

tgagctcagt cttgtcatgg caaaataaag ataataatag tgttttttta tggagttagc     6360

gtgaggatgg aaaacaatag caaaattgat tagactataa aaggtctcaa caaatagtag     6420

tagattttat catccattaa tccttccctc tcctctctta ctcatcccat cacgtatgcc     6480

tcttaatttt cccttaccta taataagagt tattcctctt attatattct tcttatagtg     6540

attctggata ttaaagtggg aatgaggggc aggccactaa cgaagaagat gtttctcaaa     6600

gaagcggggg atccactagt tctagagcgg ccaaatggcg gccgtacctt taagaccaat     6660

gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg     6720

gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt     6780

agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca     6840

ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa     6900

ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg     6960

tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa     7020

cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa     7080

taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta     7140

tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc     7200

agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag     7260

gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg     7320

acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac     7380

aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc     7440

ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc     7500

gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg     7560

tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt     7620

tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc     7680

tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg     7740

gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg     7800

agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct     7860

cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg     7920

agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg     7980

tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc     8040

aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag     8100

gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg     8160

ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt     8220

gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt     8280

tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt     8340

attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa     8400

tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag     8460

agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac     8520

aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac     8580

tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac     8640

cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac     8700

tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact     8760

tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg     8820

tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt     8880

tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat     8940

aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta     9000

gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa     9060

tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga     9120

aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac     9180

aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt     9240

tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc     9300

gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat     9360

cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag     9420

acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc     9480

cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag     9540

cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac     9600

aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg     9660

gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct     9720

atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc     9780

tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga     9840

gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga     9900

agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg     9960

cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt    10020

gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt    10080

gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc    10140

caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttgg         10195


<210>  46
<211>  10195
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LV.GLOBE.betaAS3-GLOBIN Sal1 (with restriction site for Sal1)

<400>  46
ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc atgtccaaca       60

ttaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca      120

ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct      180

ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta      240

acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac      300

ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt      360

aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag      420

tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat      480

gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat      540

gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc      600

ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt      660

ttagtgaacc ggggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac      720

tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg      780

cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga      840

aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct      900

ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact      960

ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag     1020

cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg     1080

gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt     1140

cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct     1200

acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac     1260

cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat     1320

agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac     1380

ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa     1440

aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa     1500

aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta     1560

tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc     1620

agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag     1680

tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc     1740

aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt     1800

ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg     1860

agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc     1920

aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt     1980

ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag     2040

gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc     2100

agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc     2160

ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga     2220

acggatctcg acggtatcgg ttaactttta aaagaaaagg ggggattggg gggtacagtg     2280

caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac     2340

aaattacaaa attcaaaatt ttatcggtac gtaccatgag gacagctaaa acaataagta     2400

atgtaaaata cagcatagca aaactttaac ctccaaatca agcctctact tgaatccttt     2460

tctgagggat gaataaggca taggcatcag gggctgttgc caatgtgcat tagctgtttg     2520

cagcctcacc ttctttcatg gagtttaaga tatagtgtat tttcccaagg tttgaactag     2580

ctcttcattt ctttatgttt taaatgcact gacctcccac attccctttt tagtaaaata     2640

ttcagaaata atttaaatac atcattgcaa tgaaaataaa tgttttttat taggcagaat     2700

ccagatgctc aaggcccttc ataatatccc ccagtttagt agttggactt agggaacaaa     2760

ggaaccttta atagaaattg gacagcaaga aagcgagctt agtgatactt gtgggccagg     2820

gcattagcca caccagccac cactttctga taggcagcct gcactggtgg ggtgaattct     2880

ttgccaaagt gatgggccag cacacagacc agcacgttgc ccaggagctg tgggaggaag     2940

ataagaggta tgaacatgat tagcaaaagg gcctagcttg gactcagaat aatccagcct     3000

tatcccaacc ataaaataaa agcagaatgg tagctggatt gtagctgcta ttagcaatat     3060

gaaacctctt acatcagtta caatttatat gcagaaatac cctgttactt ctccccttcc     3120

tatgacatga acttaaccat agaaaagaag gggaaagaaa acatcaaggg tcccatagac     3180

tcaccctgaa gttctcagga tccacgtgca gcttgtcaca gtgcagctca ctcagctggg     3240

caaaggtgcc cttgaggttg tccaggtgag ccaggccatc actaaaggca ccgagcactt     3300

tcttgccatg agccttcacc ttagggttgc ccataacagc atcaggagtg gacagatccc     3360

caaaggactc aaagaacctc tgggtccaag ggtagaccac cagcagccta agggtgggaa     3420

aatagaccaa taggcagaga gagtcagtgc ctatcagaaa cccaagagtc ttctctgtct     3480

ccacatgccc agtttctatt ggtctcctta aacctgtctt gtaaccttga taccaacctg     3540

cccagggcct caccaccaac ggcatccacg ttcaccttgt cccagagagc ggtcacagcg     3600

gacttctcct caggagtcag gtgcaccatg gtgtctgttt gaggttgcta gtgaacacag     3660

ttgtgtcaga agcaaatgta agcaatagat ggctctgccc tgacttttat gcccagccct     3720

ggctcctgcc ctccctgctc ctgggagtag attggccaac cctagggtgt ggctccacag     3780

ggtgaggtct aagtgatgac agccgtacct gtccttggct cttctggcac tggcttagga     3840

gttggacttc aaaccctcag ccctccctct aagatatatc tcttggcccc ataccatcag     3900

tacaaattgc tactaaaaac atcctccttt gcaagtgtat ttacacggta tcgataagct     3960

tgatatcgaa ttcctgcagc ccccttttgc cacctagctg tccaggggtg ccttaaaatg     4020

gcaaacaagg tttgttttct tttcctgttt tcatgccttc ctcttccata tccttgtttc     4080

atattaatac atgtgtatag atcctaaaaa tctatacaca tgtattaata aagcctgatt     4140

ctgccgcttc taggtataga ggccacctgc aagataaata tttgattcac aataactaat     4200

cattctatgg caattgataa caacaaatat atatatatat atatatacgt atatgtgtat     4260

atatatatat atatattcag gaaataatat attctagaat atgtcacatt ctgtctcagg     4320

catccatttt ctttatgatg ccgtttgagg tggagtttta gtcaggtggt cagcttctcc     4380

ttttttttgc catctgccct gtaagcatcc tgctggggac ccagatagga gtcatcactc     4440

taggctgaga acatctgggc acacacccta agcctcagca tgactcatca tgactcagca     4500

ttgctgtgct tgagccagaa ggtttgctta gaaggttaca cagaaccaga aggcgggggt     4560

ggggcactga ccccgacagg ggcctggcca gaactgctca tgcttggact atgggaggtc     4620

actaatggag acacacagaa atgtaacagg aactaaggaa aaactgaagc ttatttaatc     4680

agagatgagg atgctggaag ggatagaggg agctgagctt gtaaaaagta tagtaatcat     4740

tcagcaaatg gttttgaagc acctgctgga tgctaaacac tattttcagt gcttgaatca     4800

taaataagaa taaaacatgt atcttattcc ccacaagagt ccaagtaaaa aataacagtt     4860

aattataatg tgctctgtcc cccaggctgg agtgcagtgg cacgatctca gctcactgca     4920

acctccgcct cccgggttca agcaattctc ctgcctcagc caccctaata gctgggatta     4980

caggtgcaca ccaccatgcc aggctaattt ttgtactttt tgtagaggca gggtatcacc     5040

atgttgtcca agatggtctt gaactcctga gctccaagca gtccacccac ctcagcctcc     5100

caaagtgctg ggattacagg tgtgagacac catgcccaga ttttccatat ttaatagagg     5160

tatttatggg atgggggaaa agaatgtttc tctcactgtg gattatttta gagagtggag     5220

aatggtcaag atttttttaa aaattaagaa aacataagtt ggaccttgag aaatgaaaat     5280

ttattttttt gttggaggat acccattctc tatctcccat cagggcaagc tgtaaggaac     5340

tggctaagac acagtgagac agagtgactt agtcttagag gccccactgg tacgacggtc     5400

accaagcttt cattaaaaaa agtctaacca gctgcattcg actttgactg cagcagctgg     5460

ttagaaggtt ctactggagg agggtcccag cccattgcta aattaacatc aggctctgag     5520

actggcagta tatctctaac agtggttgat gctatcttct ggaacttgcc tgctacattg     5580

agaccactga cccatacata ggaagcccat agctctgtcc tgaactgtta ggccactggt     5640

ccagagagtg tgcatctcct ttgatcctca taataaccct atgagataga cacaattatt     5700

actcttactt tatagatgat gatcctgaaa acataggagt caaggcactt gcccctagct     5760

gggggtatag gggagcagtc ccatgtagta gtagaatgaa aaatgctgct atgctgtgcc     5820

tcccccacct ttcccatgtc tgccctctac tcatggtcta tctctcctgg ctcctgggag     5880

tcatggactc cacccagcac caccaacctg acctaaccac ctatctgagc ctgccagcct     5940

ataacccatc tgggccctga tagctggtgg ccagccctga ccccacccca ccctccctgg     6000

aacctctgat agacacatct ggcacaccag ctcgcaaagt caccgtgagg gtcttgtgtt     6060

tgctgagtca aaattccttg aaatccaagt ccttagagac tcctgctccc aaatttacag     6120

tcatagactt cttcatggct gtctccttta tccacagaat gattcctttg cttcattgcc     6180

ccatccatct gatcctcctc atcagtgcag cacagggccc atgagcagta gctgcagagt     6240

ctcacatagg tctggcactg cctctgacat gtccgacctt aggcaaatgc ttgactcttc     6300

tgagctcagt cttgtcatgg caaaataaag ataataatag tgttttttta tggagttagc     6360

gtgaggatgg aaaacaatag caaaattgat tagactataa aaggtctcaa caaatagtag     6420

tagattttat catccattaa tccttccctc tcctctctta ctcatcccat cacgtatgcc     6480

tcttaatttt cccttaccta taataagagt tattcctctt attatattct tcttatagtg     6540

attctggata ttaaagtggg aatgaggggc aggccactaa cgaagaagat gtttctcaaa     6600

gaagcggggg atccactagt tctagagcgg ccaaatggcg gccgtacctt taagaccaat     6660

gacttacaag gcagctgtcg accttagcca ctttttaaaa gaaaaggggg gactggaagg     6720

gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt     6780

agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca     6840

ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa     6900

ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg     6960

tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa     7020

cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa     7080

taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta     7140

tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc     7200

agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag     7260

gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg     7320

acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac     7380

aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc     7440

ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc     7500

gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg     7560

tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt     7620

tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc     7680

tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg     7740

gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg     7800

agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct     7860

cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg     7920

agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg     7980

tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc     8040

aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag     8100

gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg     8160

ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt     8220

gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt     8280

tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt     8340

attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa     8400

tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag     8460

agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac     8520

aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac     8580

tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac     8640

cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac     8700

tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact     8760

tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg     8820

tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt     8880

tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat     8940

aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta     9000

gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa     9060

tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga     9120

aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac     9180

aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt     9240

tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc     9300

gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat     9360

cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag     9420

acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc     9480

cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag     9540

cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac     9600

aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg     9660

gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct     9720

atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc     9780

tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga     9840

gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga     9900

agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg     9960

cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt    10020

gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt    10080

gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc    10140

caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttgg         10195


<210>  47
<211>  10578
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LV.GLOBE.betaAS3-GLOBIN.gRNAD-OPTIMIZED (harboring the expression
       cassette for grna D - dang scaffold - U6 promoter)

<400>  47
ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc atgtccaaca       60

ttaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca      120

ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct      180

ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta      240

acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac      300

ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt      360

aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag      420

tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat      480

gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat      540

gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc      600

ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt      660

ttagtgaacc ggggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac      720

tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg      780

cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga      840

aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct      900

ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact      960

ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag     1020

cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg     1080

gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt     1140

cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct     1200

acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac     1260

cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat     1320

agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac     1380

ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa     1440

aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa     1500

aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta     1560

tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc     1620

agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag     1680

tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc     1740

aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt     1800

ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg     1860

agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc     1920

aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt     1980

ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag     2040

gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc     2100

agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc     2160

ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga     2220

acggatctcg acggtatcgg ttaactttta aaagaaaagg ggggattggg gggtacagtg     2280

caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac     2340

aaattacaaa attcaaaatt ttatcggtac gtaccatgag gacagctaaa acaataagta     2400

atgtaaaata cagcatagca aaactttaac ctccaaatca agcctctact tgaatccttt     2460

tctgagggat gaataaggca taggcatcag gggctgttgc caatgtgcat tagctgtttg     2520

cagcctcacc ttctttcatg gagtttaaga tatagtgtat tttcccaagg tttgaactag     2580

ctcttcattt ctttatgttt taaatgcact gacctcccac attccctttt tagtaaaata     2640

ttcagaaata atttaaatac atcattgcaa tgaaaataaa tgttttttat taggcagaat     2700

ccagatgctc aaggcccttc ataatatccc ccagtttagt agttggactt agggaacaaa     2760

ggaaccttta atagaaattg gacagcaaga aagcgagctt agtgatactt gtgggccagg     2820

gcattagcca caccagccac cactttctga taggcagcct gcactggtgg ggtgaattct     2880

ttgccaaagt gatgggccag cacacagacc agcacgttgc ccaggagctg tgggaggaag     2940

ataagaggta tgaacatgat tagcaaaagg gcctagcttg gactcagaat aatccagcct     3000

tatcccaacc ataaaataaa agcagaatgg tagctggatt gtagctgcta ttagcaatat     3060

gaaacctctt acatcagtta caatttatat gcagaaatac cctgttactt ctccccttcc     3120

tatgacatga acttaaccat agaaaagaag gggaaagaaa acatcaaggg tcccatagac     3180

tcaccctgaa gttctcagga tccacgtgca gcttgtcaca gtgcagctca ctcagctggg     3240

caaaggtgcc cttgaggttg tccaggtgag ccaggccatc actaaaggca ccgagcactt     3300

tcttgccatg agccttcacc ttagggttgc ccataacagc atcaggagtg gacagatccc     3360

caaaggactc aaagaacctc tgggtccaag ggtagaccac cagcagccta agggtgggaa     3420

aatagaccaa taggcagaga gagtcagtgc ctatcagaaa cccaagagtc ttctctgtct     3480

ccacatgccc agtttctatt ggtctcctta aacctgtctt gtaaccttga taccaacctg     3540

cccagggcct caccaccaac ggcatccacg ttcaccttgt cccagagagc ggtcacagcg     3600

gacttctcct caggagtcag gtgcaccatg gtgtctgttt gaggttgcta gtgaacacag     3660

ttgtgtcaga agcaaatgta agcaatagat ggctctgccc tgacttttat gcccagccct     3720

ggctcctgcc ctccctgctc ctgggagtag attggccaac cctagggtgt ggctccacag     3780

ggtgaggtct aagtgatgac agccgtacct gtccttggct cttctggcac tggcttagga     3840

gttggacttc aaaccctcag ccctccctct aagatatatc tcttggcccc ataccatcag     3900

tacaaattgc tactaaaaac atcctccttt gcaagtgtat ttacacggta tcgataagct     3960

tgatatcgaa ttcctgcagc ccccttttgc cacctagctg tccaggggtg ccttaaaatg     4020

gcaaacaagg tttgttttct tttcctgttt tcatgccttc ctcttccata tccttgtttc     4080

atattaatac atgtgtatag atcctaaaaa tctatacaca tgtattaata aagcctgatt     4140

ctgccgcttc taggtataga ggccacctgc aagataaata tttgattcac aataactaat     4200

cattctatgg caattgataa caacaaatat atatatatat atatatacgt atatgtgtat     4260

atatatatat atatattcag gaaataatat attctagaat atgtcacatt ctgtctcagg     4320

catccatttt ctttatgatg ccgtttgagg tggagtttta gtcaggtggt cagcttctcc     4380

ttttttttgc catctgccct gtaagcatcc tgctggggac ccagatagga gtcatcactc     4440

taggctgaga acatctgggc acacacccta agcctcagca tgactcatca tgactcagca     4500

ttgctgtgct tgagccagaa ggtttgctta gaaggttaca cagaaccaga aggcgggggt     4560

ggggcactga ccccgacagg ggcctggcca gaactgctca tgcttggact atgggaggtc     4620

actaatggag acacacagaa atgtaacagg aactaaggaa aaactgaagc ttatttaatc     4680

agagatgagg atgctggaag ggatagaggg agctgagctt gtaaaaagta tagtaatcat     4740

tcagcaaatg gttttgaagc acctgctgga tgctaaacac tattttcagt gcttgaatca     4800

taaataagaa taaaacatgt atcttattcc ccacaagagt ccaagtaaaa aataacagtt     4860

aattataatg tgctctgtcc cccaggctgg agtgcagtgg cacgatctca gctcactgca     4920

acctccgcct cccgggttca agcaattctc ctgcctcagc caccctaata gctgggatta     4980

caggtgcaca ccaccatgcc aggctaattt ttgtactttt tgtagaggca gggtatcacc     5040

atgttgtcca agatggtctt gaactcctga gctccaagca gtccacccac ctcagcctcc     5100

caaagtgctg ggattacagg tgtgagacac catgcccaga ttttccatat ttaatagagg     5160

tatttatggg atgggggaaa agaatgtttc tctcactgtg gattatttta gagagtggag     5220

aatggtcaag atttttttaa aaattaagaa aacataagtt ggaccttgag aaatgaaaat     5280

ttattttttt gttggaggat acccattctc tatctcccat cagggcaagc tgtaaggaac     5340

tggctaagac acagtgagac agagtgactt agtcttagag gccccactgg tacgacggtc     5400

accaagcttt cattaaaaaa agtctaacca gctgcattcg actttgactg cagcagctgg     5460

ttagaaggtt ctactggagg agggtcccag cccattgcta aattaacatc aggctctgag     5520

actggcagta tatctctaac agtggttgat gctatcttct ggaacttgcc tgctacattg     5580

agaccactga cccatacata ggaagcccat agctctgtcc tgaactgtta ggccactggt     5640

ccagagagtg tgcatctcct ttgatcctca taataaccct atgagataga cacaattatt     5700

actcttactt tatagatgat gatcctgaaa acataggagt caaggcactt gcccctagct     5760

gggggtatag gggagcagtc ccatgtagta gtagaatgaa aaatgctgct atgctgtgcc     5820

tcccccacct ttcccatgtc tgccctctac tcatggtcta tctctcctgg ctcctgggag     5880

tcatggactc cacccagcac caccaacctg acctaaccac ctatctgagc ctgccagcct     5940

ataacccatc tgggccctga tagctggtgg ccagccctga ccccacccca ccctccctgg     6000

aacctctgat agacacatct ggcacaccag ctcgcaaagt caccgtgagg gtcttgtgtt     6060

tgctgagtca aaattccttg aaatccaagt ccttagagac tcctgctccc aaatttacag     6120

tcatagactt cttcatggct gtctccttta tccacagaat gattcctttg cttcattgcc     6180

ccatccatct gatcctcctc atcagtgcag cacagggccc atgagcagta gctgcagagt     6240

ctcacatagg tctggcactg cctctgacat gtccgacctt aggcaaatgc ttgactcttc     6300

tgagctcagt cttgtcatgg caaaataaag ataataatag tgttttttta tggagttagc     6360

gtgaggatgg aaaacaatag caaaattgat tagactataa aaggtctcaa caaatagtag     6420

tagattttat catccattaa tccttccctc tcctctctta ctcatcccat cacgtatgcc     6480

tcttaatttt cccttaccta taataagagt tattcctctt attatattct tcttatagtg     6540

attctggata ttaaagtggg aatgaggggc aggccactaa cgaagaagat gtttctcaaa     6600

gaagcggggg atccactagt tctagagcgg ccaaatggcg gccgtacctt taagaccaat     6660

gacttacaag gcagctgtcg acaggtcggg caggaagagg gcctatttcc catgattcct     6720

tcatatttgc atatacgata caaggctgtt agagagataa ttagaattaa tttgactgta     6780

aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt     6840

gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat     6900

ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgtctg ccgttactgc     6960

cctgtggttt cagagctatg ctggaaacag catagcaagt tgaaataagg ctagtccgtt     7020

atcaacttga aaaagtggca ccgagtcggt gctttttttg tcgaccttag ccacttttta     7080

aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt     7140

gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta     7200

gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc     7260

cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa     7320

atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa     7380

tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc     7440

aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg     7500

tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc     7560

ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt     7620

tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag     7680

gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc     7740

gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact     7800

taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac     7860

cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg     7920

cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc     7980

cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc     8040

ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct     8100

cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac     8160

ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac     8220

tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat     8280

ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa     8340

aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt     8400

tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa     8460

atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt     8520

attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa     8580

gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac     8640

agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt     8700

aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt     8760

cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat     8820

cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac     8880

actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg     8940

cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc     9000

ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa     9060

ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag     9120

gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct     9180

gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat     9240

ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa     9300

cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac     9360

caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc     9420

taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc     9480

cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg     9540

cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg     9600

gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca     9660

aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg     9720

cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg     9780

tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga     9840

acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac     9900

ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat     9960

ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc    10020

tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga    10080

tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc    10140

ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg    10200

gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag    10260

cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc    10320

gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc    10380

agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac    10440

tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga    10500

aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag    10560

ctggagctgc aagcttgg                                                  10578


<210>  48
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA against gamma-delta intergenic region

<400>  48
ggtgctctat acttccta                                                     18


<210>  49
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA A spacer inverted sequence

<400>  49
gaacggggtg tcccgtcatt                                                   20


<210>  50
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HBG genes inverted sequence OFF-TARGET gRNA A

<400>  50
gaacggggtg tccgaacact                                                   20


<210>  51
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HBD gene inverted sequence OFF-TARGET gRNA A

<400>  51
aaacggggtg tcccgtaact                                                   20


<210>  52
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA B spacer inverted sequence

<400>  52
cattgccgtc tgaagaggag                                                   20


<210>  53
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HBG genes inverted sequence OFF-TARGET gRNA B

<400>  53
tatcatcgga acaggaggag                                                   20


<210>  54
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HBD gene inverted sequence OFF-TARGET gRNA B

<400>  54
aactgtcgtc agaagaggag                                                   20


<210>  55
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA D spacer inverted sequence

<400>  55
agacggcaat gacgggaca                                                    19


<210>  56
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HBG genes inverted sequence OFF-TARGET gRNA D

<400>  56
cgatgatagt gttcggaca                                                    19


<210>  57
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HBD gene inverted sequence OFF-TARGET gRNA D

<400>  57
tgacgacagt tacgggaca                                                    19


<210>  58
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA E spacer inverted sequence

<400>  58
ttccacttgc acctacttca                                                   20


<210>  59
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HBG genes inverted sequence OFF-TARGET gRNA E

<400>  59
ttccacttac accttctacg                                                   20


<210>  60
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HBD gene inverted sequence OFF-TARGET gRNA E

<400>  60
tttcacttgc acctacgtca                                                   20


<210>  61
<211>  101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ORIGINAL gRNA SCAFFOLD sequence


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  61
nnnnnnnnnn nnnnnnnnnn guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu u                          101


<210>  62
<211>  112
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OPTIMIZED gRNA SCAFFOLD sequence


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  62
nnnnnnnnnn nnnnnnnnnn guuucagagc uaugcuggaa acagcauagc aaguugaaau       60

aaggcuaguc cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu uu              112


<210>  63
<211>  101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ORIGINAL SCAFFOLD gRNA B sequence

<400>  63
guaacggcag acuucuccuc guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu u                          101


<210>  64
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ORIGINAL SCAFFOLD gRNA D sequence

<400>  64
ucugccguua cugcccugug uuuuagagcu agaaauagca aguuaaaaua aggcuagucc       60

guuaucaacu ugaaaaagug gcaccgaguc ggugcuuuuu                            100


<210>  65
<211>  101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ORIGINAL SCAFFOLD gRNA E sequence

<400>  65
aaggugaacg uggaugaagu guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu u                          101


<210>  66
<211>  113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OPTIMIZED SCAFFOLD gRNA B sequence

<400>  66
guaacggcag acuucuccuc gguuucagag cuaugcugga aacagcauag caaguugaaa       60

uaaggcuagu ccguuaucaa cuugaaaaag uggcaccgag ucggugcuuu uuu             113


<210>  67
<211>  112
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OPTIMIZED SCAFFOLD gRNA D sequence

<400>  67
ucugccguua cugcccugug guuucagagc uaugcuggaa acagcauagc aaguugaaau       60

aaggcuaguc cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu uu              112


<210>  68
<211>  114
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  OPTIMIZED SCAFFOLD gRNA E sequence

<400>  68
gaaggugaac guggaugaag ugguuucaga gcuaugcugg aaacagcaua gcaaguugaa       60

auaaggcuag uccguuauca acuugaaaaa guggcaccga gucggugcuu uuuu            114


<210>  69
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HBB gene SCD sequence

<400>  69
cctgtggaga agtctgccgt tactgccctg tggg                                   34


<210>  70
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Beta-S globin protein sequence

<400>  70

Pro Val Glu Lys Ser Ala Val Thr Ala Leu Trp 
1               5                   10      


<210>  71
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HBB-AS3 modified sequence

<400>  71
cctgaggaga agtccgctgt gaccgctctc tggg                                   34


<210>  72
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Beta-AS3 globin sequence

<400>  72

Pro Glu Glu Lys Ser Ala Val Thr Ala Leu Trp 
1               5                   10      


<210>  73
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA 13bp-del spacer

<400>  73
cttgtcaagg ctattggtca                                                   20


<210>  74
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA BCL11A spacer

<400>  74
cacaggctcc aggaagggtt                                                   20


<210>  75
<211>  10584
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LV.GLOBE-AS3modified.gRNA-BCL11Aenhancer

<400>  75
ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc atgtccaaca       60

ttaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca      120

ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct      180

ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta      240

acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac      300

ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt      360

aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag      420

tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat      480

gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat      540

gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc      600

ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt      660

ttagtgaacc ggggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac      720

tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg      780

cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga      840

aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct      900

ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact      960

ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag     1020

cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg     1080

gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt     1140

cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct     1200

acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac     1260

cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat     1320

agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac     1380

ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa     1440

aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa     1500

aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta     1560

tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc     1620

agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag     1680

tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc     1740

aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt     1800

ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg     1860

agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc     1920

aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt     1980

ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag     2040

gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc     2100

agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc     2160

ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga     2220

acggatctcg acggtatcgg ttaactttta aaagaaaagg ggggattggg gggtacagtg     2280

caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac     2340

aaattacaaa attcaaaatt ttatcggtac gtaccatgag gacagctaaa acaataagta     2400

atgtaaaata cagcatagca aaactttaac ctccaaatca agcctctact tgaatccttt     2460

tctgagggat gaataaggca taggcatcag gggctgttgc caatgtgcat tagctgtttg     2520

cagcctcacc ttctttcatg gagtttaaga tatagtgtat tttcccaagg tttgaactag     2580

ctcttcattt ctttatgttt taaatgcact gacctcccac attccctttt tagtaaaata     2640

ttcagaaata atttaaatac atcattgcaa tgaaaataaa tgttttttat taggcagaat     2700

ccagatgctc aaggcccttc ataatatccc ccagtttagt agttggactt agggaacaaa     2760

ggaaccttta atagaaattg gacagcaaga aagcgagctt agtgatactt gtgggccagg     2820

gcattagcca caccagccac cactttctga taggcagcct gcactggtgg ggtgaattct     2880

ttgccaaagt gatgggccag cacacagacc agcacgttgc ccaggagctg tgggaggaag     2940

ataagaggta tgaacatgat tagcaaaagg gcctagcttg gactcagaat aatccagcct     3000

tatcccaacc ataaaataaa agcagaatgg tagctggatt gtagctgcta ttagcaatat     3060

gaaacctctt acatcagtta caatttatat gcagaaatac cctgttactt ctccccttcc     3120

tatgacatga acttaaccat agaaaagaag gggaaagaaa acatcaaggg tcccatagac     3180

tcaccctgaa gttctcagga tccacgtgca gcttgtcaca gtgcagctca ctcagctggg     3240

caaaggtgcc cttgaggttg tccaggtgag ccaggccatc actaaaggca ccgagcactt     3300

tcttgccatg agccttcacc ttagggttgc ccataacagc atcaggagtg gacagatccc     3360

caaaggactc aaagaacctc tgggtccaag ggtagaccac cagcagccta agggtgggaa     3420

aatagaccaa taggcagaga gagtcagtgc ctatcagaaa cccaagagtc ttctctgtct     3480

ccacatgccc agtttctatt ggtctcctta aacctgtctt gtaaccttga taccaacctg     3540

cccagggcct caccaccaac ggcatccacg ttcaccttgt cccagagagc ggtcacagcg     3600

gacttctcct caggagtcag gtgcaccatg gtgtctgttt gaggttgcta gtgaacacag     3660

ttgtgtcaga agcaaatgta agcaatagat ggctctgccc tgacttttat gcccagccct     3720

ggctcctgcc ctccctgctc ctgggagtag attggccaac cctagggtgt ggctccacag     3780

ggtgaggtct aagtgatgac agccgtacct gtccttggct cttctggcac tggcttagga     3840

gttggacttc aaaccctcag ccctccctct aagatatatc tcttggcccc ataccatcag     3900

tacaaattgc tactaaaaac atcctccttt gcaagtgtat ttacacggta tcgataagct     3960

tgatatcgaa ttcctgcagc ccccttttgc cacctagctg tccaggggtg ccttaaaatg     4020

gcaaacaagg tttgttttct tttcctgttt tcatgccttc ctcttccata tccttgtttc     4080

atattaatac atgtgtatag atcctaaaaa tctatacaca tgtattaata aagcctgatt     4140

ctgccgcttc taggtataga ggccacctgc aagataaata tttgattcac aataactaat     4200

cattctatgg caattgataa caacaaatat atatatatat atatatacgt atatgtgtat     4260

atatatatat atatattcag gaaataatat attctagaat atgtcacatt ctgtctcagg     4320

catccatttt ctttatgatg ccgtttgagg tggagtttta gtcaggtggt cagcttctcc     4380

ttttttttgc catctgccct gtaagcatcc tgctggggac ccagatagga gtcatcactc     4440

taggctgaga acatctgggc acacacccta agcctcagca tgactcatca tgactcagca     4500

ttgctgtgct tgagccagaa ggtttgctta gaaggttaca cagaaccaga aggcgggggt     4560

ggggcactga ccccgacagg ggcctggcca gaactgctca tgcttggact atgggaggtc     4620

actaatggag acacacagaa atgtaacagg aactaaggaa aaactgaagc ttatttaatc     4680

agagatgagg atgctggaag ggatagaggg agctgagctt gtaaaaagta tagtaatcat     4740

tcagcaaatg gttttgaagc acctgctgga tgctaaacac tattttcagt gcttgaatca     4800

taaataagaa taaaacatgt atcttattcc ccacaagagt ccaagtaaaa aataacagtt     4860

aattataatg tgctctgtcc cccaggctgg agtgcagtgg cacgatctca gctcactgca     4920

acctccgcct cccgggttca agcaattctc ctgcctcagc caccctaata gctgggatta     4980

caggtgcaca ccaccatgcc aggctaattt ttgtactttt tgtagaggca gggtatcacc     5040

atgttgtcca agatggtctt gaactcctga gctccaagca gtccacccac ctcagcctcc     5100

caaagtgctg ggattacagg tgtgagacac catgcccaga ttttccatat ttaatagagg     5160

tatttatggg atgggggaaa agaatgtttc tctcactgtg gattatttta gagagtggag     5220

aatggtcaag atttttttaa aaattaagaa aacataagtt ggaccttgag aaatgaaaat     5280

ttattttttt gttggaggat acccattctc tatctcccat cagggcaagc tgtaaggaac     5340

tggctaagac acagtgagac agagtgactt agtcttagag gccccactgg tacgacggtc     5400

accaagcttt cattaaaaaa agtctaacca gctgcattcg actttgactg cagcagctgg     5460

ttagaaggtt ctactggagg agggtcccag cccattgcta aattaacatc aggctctgag     5520

actggcagta tatctctaac agtggttgat gctatcttct ggaacttgcc tgctacattg     5580

agaccactga cccatacata ggaagcccat agctctgtcc tgaactgtta ggccactggt     5640

ccagagagtg tgcatctcct ttgatcctca taataaccct atgagataga cacaattatt     5700

actcttactt tatagatgat gatcctgaaa acataggagt caaggcactt gcccctagct     5760

gggggtatag gggagcagtc ccatgtagta gtagaatgaa aaatgctgct atgctgtgcc     5820

tcccccacct ttcccatgtc tgccctctac tcatggtcta tctctcctgg ctcctgggag     5880

tcatggactc cacccagcac caccaacctg acctaaccac ctatctgagc ctgccagcct     5940

ataacccatc tgggccctga tagctggtgg ccagccctga ccccacccca ccctccctgg     6000

aacctctgat agacacatct ggcacaccag ctcgcaaagt caccgtgagg gtcttgtgtt     6060

tgctgagtca aaattccttg aaatccaagt ccttagagac tcctgctccc aaatttacag     6120

tcatagactt cttcatggct gtctccttta tccacagaat gattcctttg cttcattgcc     6180

ccatccatct gatcctcctc atcagtgcag cacagggccc atgagcagta gctgcagagt     6240

ctcacatagg tctggcactg cctctgacat gtccgacctt aggcaaatgc ttgactcttc     6300

tgagctcagt cttgtcatgg caaaataaag ataataatag tgttttttta tggagttagc     6360

gtgaggatgg aaaacaatag caaaattgat tagactataa aaggtctcaa caaatagtag     6420

tagattttat catccattaa tccttccctc tcctctctta ctcatcccat cacgtatgcc     6480

tcttaatttt cccttaccta taataagagt tattcctctt attatattct tcttatagtg     6540

attctggata ttaaagtggg aatgaggggc aggccactaa cgaagaagat gtttctcaaa     6600

gaagcggggg atccgtcgac aggtcgggca ggaagagggc ctatttccca tgattccttc     6660

atatttgcat atacgataca aggctgttag agagataatt agaattaatt tgactgtaaa     6720

cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc     6780

agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt     6840

cgatttcttg gctttatata tcttgtggaa aggacgaaac accgcacagg ctccaggaag     6900

ggttgtttca gagctatgct ggaaacagca tagcaagttg aaataaggct agtccgttat     6960

caacttgaaa aagtggcacc gagtcggtgc tttttttgtc gacactagtt ctagagcggc     7020

caaatggcgg ccgtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac     7080

tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg     7140

ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc     7200

taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg     7260

tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg     7320

tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca     7380

aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa     7440

taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt     7500

ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa     7560

ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac     7620

taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt     7680

agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta     7740

ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac     7800

ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc     7860

ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg     7920

tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc     7980

cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg     8040

ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg     8100

gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg     8160

atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt     8220

ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt     8280

gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt     8340

taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc     8400

cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc     8460

tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc     8520

gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg     8580

gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat     8640

ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc     8700

acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa     8760

ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa     8820

aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt     8880

gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct     8940

tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat     9000

gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg     9060

cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg     9120

atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt     9180

attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg     9240

ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg     9300

gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg     9360

tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa     9420

aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt     9480

tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt     9540

tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt     9600

ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag     9660

ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta     9720

gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat     9780

aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg     9840

ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg     9900

agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac     9960

aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga    10020

aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt    10080

ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta    10140

cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat    10200

tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg    10260

accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct    10320

ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa    10380

gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct    10440

ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac    10500

acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa    10560

caaaagctgg agctgcaagc ttgg                                           10584


<210>  76
<211>  10584
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LV.GLOBE-AS3modified.gRNA-13bp-del

<400>  76
ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc atgtccaaca       60

ttaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca      120

ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct      180

ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta      240

acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac      300

ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt      360

aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag      420

tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat      480

gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat      540

gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc      600

ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt      660

ttagtgaacc ggggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac      720

tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg      780

cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga      840

aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct      900

ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact      960

ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag     1020

cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg     1080

gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt     1140

cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct     1200

acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac     1260

cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat     1320

agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac     1380

ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa     1440

aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa     1500

aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta     1560

tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc     1620

agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag     1680

tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc     1740

aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt     1800

ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg     1860

agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc     1920

aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt     1980

ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag     2040

gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc     2100

agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc     2160

ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga     2220

acggatctcg acggtatcgg ttaactttta aaagaaaagg ggggattggg gggtacagtg     2280

caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac     2340

aaattacaaa attcaaaatt ttatcggtac gtaccatgag gacagctaaa acaataagta     2400

atgtaaaata cagcatagca aaactttaac ctccaaatca agcctctact tgaatccttt     2460

tctgagggat gaataaggca taggcatcag gggctgttgc caatgtgcat tagctgtttg     2520

cagcctcacc ttctttcatg gagtttaaga tatagtgtat tttcccaagg tttgaactag     2580

ctcttcattt ctttatgttt taaatgcact gacctcccac attccctttt tagtaaaata     2640

ttcagaaata atttaaatac atcattgcaa tgaaaataaa tgttttttat taggcagaat     2700

ccagatgctc aaggcccttc ataatatccc ccagtttagt agttggactt agggaacaaa     2760

ggaaccttta atagaaattg gacagcaaga aagcgagctt agtgatactt gtgggccagg     2820

gcattagcca caccagccac cactttctga taggcagcct gcactggtgg ggtgaattct     2880

ttgccaaagt gatgggccag cacacagacc agcacgttgc ccaggagctg tgggaggaag     2940

ataagaggta tgaacatgat tagcaaaagg gcctagcttg gactcagaat aatccagcct     3000

tatcccaacc ataaaataaa agcagaatgg tagctggatt gtagctgcta ttagcaatat     3060

gaaacctctt acatcagtta caatttatat gcagaaatac cctgttactt ctccccttcc     3120

tatgacatga acttaaccat agaaaagaag gggaaagaaa acatcaaggg tcccatagac     3180

tcaccctgaa gttctcagga tccacgtgca gcttgtcaca gtgcagctca ctcagctggg     3240

caaaggtgcc cttgaggttg tccaggtgag ccaggccatc actaaaggca ccgagcactt     3300

tcttgccatg agccttcacc ttagggttgc ccataacagc atcaggagtg gacagatccc     3360

caaaggactc aaagaacctc tgggtccaag ggtagaccac cagcagccta agggtgggaa     3420

aatagaccaa taggcagaga gagtcagtgc ctatcagaaa cccaagagtc ttctctgtct     3480

ccacatgccc agtttctatt ggtctcctta aacctgtctt gtaaccttga taccaacctg     3540

cccagggcct caccaccaac ggcatccacg ttcaccttgt cccagagagc ggtcacagcg     3600

gacttctcct caggagtcag gtgcaccatg gtgtctgttt gaggttgcta gtgaacacag     3660

ttgtgtcaga agcaaatgta agcaatagat ggctctgccc tgacttttat gcccagccct     3720

ggctcctgcc ctccctgctc ctgggagtag attggccaac cctagggtgt ggctccacag     3780

ggtgaggtct aagtgatgac agccgtacct gtccttggct cttctggcac tggcttagga     3840

gttggacttc aaaccctcag ccctccctct aagatatatc tcttggcccc ataccatcag     3900

tacaaattgc tactaaaaac atcctccttt gcaagtgtat ttacacggta tcgataagct     3960

tgatatcgaa ttcctgcagc ccccttttgc cacctagctg tccaggggtg ccttaaaatg     4020

gcaaacaagg tttgttttct tttcctgttt tcatgccttc ctcttccata tccttgtttc     4080

atattaatac atgtgtatag atcctaaaaa tctatacaca tgtattaata aagcctgatt     4140

ctgccgcttc taggtataga ggccacctgc aagataaata tttgattcac aataactaat     4200

cattctatgg caattgataa caacaaatat atatatatat atatatacgt atatgtgtat     4260

atatatatat atatattcag gaaataatat attctagaat atgtcacatt ctgtctcagg     4320

catccatttt ctttatgatg ccgtttgagg tggagtttta gtcaggtggt cagcttctcc     4380

ttttttttgc catctgccct gtaagcatcc tgctggggac ccagatagga gtcatcactc     4440

taggctgaga acatctgggc acacacccta agcctcagca tgactcatca tgactcagca     4500

ttgctgtgct tgagccagaa ggtttgctta gaaggttaca cagaaccaga aggcgggggt     4560

ggggcactga ccccgacagg ggcctggcca gaactgctca tgcttggact atgggaggtc     4620

actaatggag acacacagaa atgtaacagg aactaaggaa aaactgaagc ttatttaatc     4680

agagatgagg atgctggaag ggatagaggg agctgagctt gtaaaaagta tagtaatcat     4740

tcagcaaatg gttttgaagc acctgctgga tgctaaacac tattttcagt gcttgaatca     4800

taaataagaa taaaacatgt atcttattcc ccacaagagt ccaagtaaaa aataacagtt     4860

aattataatg tgctctgtcc cccaggctgg agtgcagtgg cacgatctca gctcactgca     4920

acctccgcct cccgggttca agcaattctc ctgcctcagc caccctaata gctgggatta     4980

caggtgcaca ccaccatgcc aggctaattt ttgtactttt tgtagaggca gggtatcacc     5040

atgttgtcca agatggtctt gaactcctga gctccaagca gtccacccac ctcagcctcc     5100

caaagtgctg ggattacagg tgtgagacac catgcccaga ttttccatat ttaatagagg     5160

tatttatggg atgggggaaa agaatgtttc tctcactgtg gattatttta gagagtggag     5220

aatggtcaag atttttttaa aaattaagaa aacataagtt ggaccttgag aaatgaaaat     5280

ttattttttt gttggaggat acccattctc tatctcccat cagggcaagc tgtaaggaac     5340

tggctaagac acagtgagac agagtgactt agtcttagag gccccactgg tacgacggtc     5400

accaagcttt cattaaaaaa agtctaacca gctgcattcg actttgactg cagcagctgg     5460

ttagaaggtt ctactggagg agggtcccag cccattgcta aattaacatc aggctctgag     5520

actggcagta tatctctaac agtggttgat gctatcttct ggaacttgcc tgctacattg     5580

agaccactga cccatacata ggaagcccat agctctgtcc tgaactgtta ggccactggt     5640

ccagagagtg tgcatctcct ttgatcctca taataaccct atgagataga cacaattatt     5700

actcttactt tatagatgat gatcctgaaa acataggagt caaggcactt gcccctagct     5760

gggggtatag gggagcagtc ccatgtagta gtagaatgaa aaatgctgct atgctgtgcc     5820

tcccccacct ttcccatgtc tgccctctac tcatggtcta tctctcctgg ctcctgggag     5880

tcatggactc cacccagcac caccaacctg acctaaccac ctatctgagc ctgccagcct     5940

ataacccatc tgggccctga tagctggtgg ccagccctga ccccacccca ccctccctgg     6000

aacctctgat agacacatct ggcacaccag ctcgcaaagt caccgtgagg gtcttgtgtt     6060

tgctgagtca aaattccttg aaatccaagt ccttagagac tcctgctccc aaatttacag     6120

tcatagactt cttcatggct gtctccttta tccacagaat gattcctttg cttcattgcc     6180

ccatccatct gatcctcctc atcagtgcag cacagggccc atgagcagta gctgcagagt     6240

ctcacatagg tctggcactg cctctgacat gtccgacctt aggcaaatgc ttgactcttc     6300

tgagctcagt cttgtcatgg caaaataaag ataataatag tgttttttta tggagttagc     6360

gtgaggatgg aaaacaatag caaaattgat tagactataa aaggtctcaa caaatagtag     6420

tagattttat catccattaa tccttccctc tcctctctta ctcatcccat cacgtatgcc     6480

tcttaatttt cccttaccta taataagagt tattcctctt attatattct tcttatagtg     6540

attctggata ttaaagtggg aatgaggggc aggccactaa cgaagaagat gtttctcaaa     6600

gaagcggggg atccgtcgac aggtcgggca ggaagagggc ctatttccca tgattccttc     6660

atatttgcat atacgataca aggctgttag agagataatt agaattaatt tgactgtaaa     6720

cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc     6780

agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt     6840

cgatttcttg gctttatata tcttgtggaa aggacgaaac accgcttgtc aaggctattg     6900

gtcagtttca gagctatgct ggaaacagca tagcaagttg aaataaggct agtccgttat     6960

caacttgaaa aagtggcacc gagtcggtgc tttttttgtc gacactagtt ctagagcggc     7020

caaatggcgg ccgtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac     7080

tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg     7140

ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc     7200

taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg     7260

tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg     7320

tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca     7380

aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa     7440

taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt     7500

ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa     7560

ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac     7620

taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt     7680

agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta     7740

ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac     7800

ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc     7860

ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg     7920

tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc     7980

cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg     8040

ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg     8100

gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg     8160

atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt     8220

ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt     8280

gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt     8340

taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc     8400

cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc     8460

tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc     8520

gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg     8580

gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat     8640

ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc     8700

acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa     8760

ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa     8820

aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt     8880

gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct     8940

tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat     9000

gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg     9060

cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg     9120

atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt     9180

attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg     9240

ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg     9300

gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg     9360

tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa     9420

aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt     9480

tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt     9540

tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt     9600

ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag     9660

ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta     9720

gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat     9780

aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg     9840

ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg     9900

agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac     9960

aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga    10020

aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt    10080

ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta    10140

cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat    10200

tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg    10260

accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct    10320

ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa    10380

gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct    10440

ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac    10500

acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa    10560

caaaagctgg agctgcaagc ttgg                                           10584


<210>  77
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BCL11A-TIDE FORWARD

<400>  77
tggacagccc gacagatgaa                                                   20


<210>  78
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BCL11A-TIDE REVERSE

<400>  78
aaaagcgata cagggctggc                                                   20


<210>  79
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  13bp-del-TIDE FORWARD

<400>  79
aaaaacggct gacaaaagaa gtcctggtat                                        30


<210>  80
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  13bp-del-TIDE REVERSE

<400>  80
ataacctcag acgttccaga agcgagtgtg                                        30


<210>  81
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HBG1 + HBG2   FORWARD

<400>  81
cctgtcctct gcctctgcc                                                    19


<210>  82
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HBG1 + HBG2 REVERSE

<400>  82
ggattgccaa aacggtcac                                                    19


<210>  83
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HBB FORWARD

<400>  83
aagggcacct ttgccaca                                                     18


<210>  84
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HBB REVERSE

<400>  84
gccaccactt tctgataggc ag                                                22


<210>  85
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HBD FORWARD

<400>  85
caagggcact ttttctcag                                                    19


<210>  86
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HBD REVERSE

<400>  86
aattccttgc caaagttgc                                                    19


<210>  87
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BCL11A FORWARD

<400>  87
aaccccagca cttaagcaaa                                                   20


<210>  88
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BCL11A REVERSE

<400>  88
ggaggtcatg atccccttct                                                   20


<210>  89
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BCL11AXL FORWARD

<400>  89
atgcgagctg tgcaactatg                                                   20


<210>  90
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BCL11AXL REVERSE

<400>  90
gtaaacgtcc ttccccacct                                                   20


<210>  91
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAPDH FORWARD

<400>  91
cttcattgac ctcaactaca tggttt                                            26


<210>  92
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAPDH REVERSE

<400>  92
tgggatttcc attgatgaca ag                                                22


<210>  93
<211>  10584
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LV.GLOBE-AS3modified.gRNA-luciferase

<400>  93
ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc atgtccaaca       60

ttaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca      120

ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct      180

ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta      240

acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac      300

ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt      360

aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag      420

tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat      480

gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat      540

gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc      600

ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt      660

ttagtgaacc ggggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac      720

tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg      780

cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga      840

aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct      900

ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact      960

ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag     1020

cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg     1080

gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt     1140

cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct     1200

acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac     1260

cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat     1320

agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac     1380

ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa     1440

aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa     1500

aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta     1560

tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc     1620

agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag     1680

tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc     1740

aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt     1800

ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg     1860

agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc     1920

aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt     1980

ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag     2040

gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc     2100

agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc     2160

ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga     2220

acggatctcg acggtatcgg ttaactttta aaagaaaagg ggggattggg gggtacagtg     2280

caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac     2340

aaattacaaa attcaaaatt ttatcggtac gtaccatgag gacagctaaa acaataagta     2400

atgtaaaata cagcatagca aaactttaac ctccaaatca agcctctact tgaatccttt     2460

tctgagggat gaataaggca taggcatcag gggctgttgc caatgtgcat tagctgtttg     2520

cagcctcacc ttctttcatg gagtttaaga tatagtgtat tttcccaagg tttgaactag     2580

ctcttcattt ctttatgttt taaatgcact gacctcccac attccctttt tagtaaaata     2640

ttcagaaata atttaaatac atcattgcaa tgaaaataaa tgttttttat taggcagaat     2700

ccagatgctc aaggcccttc ataatatccc ccagtttagt agttggactt agggaacaaa     2760

ggaaccttta atagaaattg gacagcaaga aagcgagctt agtgatactt gtgggccagg     2820

gcattagcca caccagccac cactttctga taggcagcct gcactggtgg ggtgaattct     2880

ttgccaaagt gatgggccag cacacagacc agcacgttgc ccaggagctg tgggaggaag     2940

ataagaggta tgaacatgat tagcaaaagg gcctagcttg gactcagaat aatccagcct     3000

tatcccaacc ataaaataaa agcagaatgg tagctggatt gtagctgcta ttagcaatat     3060

gaaacctctt acatcagtta caatttatat gcagaaatac cctgttactt ctccccttcc     3120

tatgacatga acttaaccat agaaaagaag gggaaagaaa acatcaaggg tcccatagac     3180

tcaccctgaa gttctcagga tccacgtgca gcttgtcaca gtgcagctca ctcagctggg     3240

caaaggtgcc cttgaggttg tccaggtgag ccaggccatc actaaaggca ccgagcactt     3300

tcttgccatg agccttcacc ttagggttgc ccataacagc atcaggagtg gacagatccc     3360

caaaggactc aaagaacctc tgggtccaag ggtagaccac cagcagccta agggtgggaa     3420

aatagaccaa taggcagaga gagtcagtgc ctatcagaaa cccaagagtc ttctctgtct     3480

ccacatgccc agtttctatt ggtctcctta aacctgtctt gtaaccttga taccaacctg     3540

cccagggcct caccaccaac ggcatccacg ttcaccttgt cccagagagc ggtcacagcg     3600

gacttctcct caggagtcag gtgcaccatg gtgtctgttt gaggttgcta gtgaacacag     3660

ttgtgtcaga agcaaatgta agcaatagat ggctctgccc tgacttttat gcccagccct     3720

ggctcctgcc ctccctgctc ctgggagtag attggccaac cctagggtgt ggctccacag     3780

ggtgaggtct aagtgatgac agccgtacct gtccttggct cttctggcac tggcttagga     3840

gttggacttc aaaccctcag ccctccctct aagatatatc tcttggcccc ataccatcag     3900

tacaaattgc tactaaaaac atcctccttt gcaagtgtat ttacacggta tcgataagct     3960

tgatatcgaa ttcctgcagc ccccttttgc cacctagctg tccaggggtg ccttaaaatg     4020

gcaaacaagg tttgttttct tttcctgttt tcatgccttc ctcttccata tccttgtttc     4080

atattaatac atgtgtatag atcctaaaaa tctatacaca tgtattaata aagcctgatt     4140

ctgccgcttc taggtataga ggccacctgc aagataaata tttgattcac aataactaat     4200

cattctatgg caattgataa caacaaatat atatatatat atatatacgt atatgtgtat     4260

atatatatat atatattcag gaaataatat attctagaat atgtcacatt ctgtctcagg     4320

catccatttt ctttatgatg ccgtttgagg tggagtttta gtcaggtggt cagcttctcc     4380

ttttttttgc catctgccct gtaagcatcc tgctggggac ccagatagga gtcatcactc     4440

taggctgaga acatctgggc acacacccta agcctcagca tgactcatca tgactcagca     4500

ttgctgtgct tgagccagaa ggtttgctta gaaggttaca cagaaccaga aggcgggggt     4560

ggggcactga ccccgacagg ggcctggcca gaactgctca tgcttggact atgggaggtc     4620

actaatggag acacacagaa atgtaacagg aactaaggaa aaactgaagc ttatttaatc     4680

agagatgagg atgctggaag ggatagaggg agctgagctt gtaaaaagta tagtaatcat     4740

tcagcaaatg gttttgaagc acctgctgga tgctaaacac tattttcagt gcttgaatca     4800

taaataagaa taaaacatgt atcttattcc ccacaagagt ccaagtaaaa aataacagtt     4860

aattataatg tgctctgtcc cccaggctgg agtgcagtgg cacgatctca gctcactgca     4920

acctccgcct cccgggttca agcaattctc ctgcctcagc caccctaata gctgggatta     4980

caggtgcaca ccaccatgcc aggctaattt ttgtactttt tgtagaggca gggtatcacc     5040

atgttgtcca agatggtctt gaactcctga gctccaagca gtccacccac ctcagcctcc     5100

caaagtgctg ggattacagg tgtgagacac catgcccaga ttttccatat ttaatagagg     5160

tatttatggg atgggggaaa agaatgtttc tctcactgtg gattatttta gagagtggag     5220

aatggtcaag atttttttaa aaattaagaa aacataagtt ggaccttgag aaatgaaaat     5280

ttattttttt gttggaggat acccattctc tatctcccat cagggcaagc tgtaaggaac     5340

tggctaagac acagtgagac agagtgactt agtcttagag gccccactgg tacgacggtc     5400

accaagcttt cattaaaaaa agtctaacca gctgcattcg actttgactg cagcagctgg     5460

ttagaaggtt ctactggagg agggtcccag cccattgcta aattaacatc aggctctgag     5520

actggcagta tatctctaac agtggttgat gctatcttct ggaacttgcc tgctacattg     5580

agaccactga cccatacata ggaagcccat agctctgtcc tgaactgtta ggccactggt     5640

ccagagagtg tgcatctcct ttgatcctca taataaccct atgagataga cacaattatt     5700

actcttactt tatagatgat gatcctgaaa acataggagt caaggcactt gcccctagct     5760

gggggtatag gggagcagtc ccatgtagta gtagaatgaa aaatgctgct atgctgtgcc     5820

tcccccacct ttcccatgtc tgccctctac tcatggtcta tctctcctgg ctcctgggag     5880

tcatggactc cacccagcac caccaacctg acctaaccac ctatctgagc ctgccagcct     5940

ataacccatc tgggccctga tagctggtgg ccagccctga ccccacccca ccctccctgg     6000

aacctctgat agacacatct ggcacaccag ctcgcaaagt caccgtgagg gtcttgtgtt     6060

tgctgagtca aaattccttg aaatccaagt ccttagagac tcctgctccc aaatttacag     6120

tcatagactt cttcatggct gtctccttta tccacagaat gattcctttg cttcattgcc     6180

ccatccatct gatcctcctc atcagtgcag cacagggccc atgagcagta gctgcagagt     6240

ctcacatagg tctggcactg cctctgacat gtccgacctt aggcaaatgc ttgactcttc     6300

tgagctcagt cttgtcatgg caaaataaag ataataatag tgttttttta tggagttagc     6360

gtgaggatgg aaaacaatag caaaattgat tagactataa aaggtctcaa caaatagtag     6420

tagattttat catccattaa tccttccctc tcctctctta ctcatcccat cacgtatgcc     6480

tcttaatttt cccttaccta taataagagt tattcctctt attatattct tcttatagtg     6540

attctggata ttaaagtggg aatgaggggc aggccactaa cgaagaagat gtttctcaaa     6600

gaagcggggg atccgtcgac aggtcgggca ggaagagggc ctatttccca tgattccttc     6660

atatttgcat atacgataca aggctgttag agagataatt agaattaatt tgactgtaaa     6720

cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc     6780

agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt     6840

cgatttcttg gctttatata tcttgtggaa aggacgaaac accgcttcga aatgtccgtt     6900

cggtgtttca gagctatgct ggaaacagca tagcaagttg aaataaggct agtccgttat     6960

caacttgaaa aagtggcacc gagtcggtgc tttttttgtc gacactagtt ctagagcggc     7020

caaatggcgg ccgtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac     7080

tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg     7140

ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc     7200

taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg     7260

tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg     7320

tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca     7380

aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa     7440

taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt     7500

ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa     7560

ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac     7620

taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt     7680

agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta     7740

ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac     7800

ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc     7860

ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg     7920

tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc     7980

cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg     8040

ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg     8100

gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg     8160

atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt     8220

ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt     8280

gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt     8340

taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc     8400

cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc     8460

tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc     8520

gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg     8580

gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat     8640

ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc     8700

acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa     8760

ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa     8820

aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt     8880

gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct     8940

tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat     9000

gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg     9060

cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg     9120

atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt     9180

attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg     9240

ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg     9300

gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg     9360

tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa     9420

aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt     9480

tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt     9540

tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt     9600

ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag     9660

ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta     9720

gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat     9780

aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg     9840

ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg     9900

agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac     9960

aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga    10020

aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt    10080

ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta    10140

cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat    10200

tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg    10260

accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct    10320

ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa    10380

gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct    10440

ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac    10500

acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa    10560

caaaagctgg agctgcaagc ttgg                                           10584


<210>  94
<211>  10583
<212>  DNA
<213>  LV.GLOBE-AS3modified.gRNAD

<400>  94
ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc atgtccaaca       60

ttaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca      120

ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct      180

ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta      240

acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac      300

ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt      360

aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag      420

tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat      480

gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat      540

gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc      600

ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt      660

ttagtgaacc ggggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac      720

tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg      780

cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga      840

aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct      900

ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact      960

ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag     1020

cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg     1080

gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt     1140

cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct     1200

acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac     1260

cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat     1320

agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac     1380

ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa     1440

aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa     1500

aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta     1560

tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc     1620

agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag     1680

tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc     1740

aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt     1800

ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg     1860

agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc     1920

aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt     1980

ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag     2040

gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc     2100

agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc     2160

ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga     2220

acggatctcg acggtatcgg ttaactttta aaagaaaagg ggggattggg gggtacagtg     2280

caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac     2340

aaattacaaa attcaaaatt ttatcggtac gtaccatgag gacagctaaa acaataagta     2400

atgtaaaata cagcatagca aaactttaac ctccaaatca agcctctact tgaatccttt     2460

tctgagggat gaataaggca taggcatcag gggctgttgc caatgtgcat tagctgtttg     2520

cagcctcacc ttctttcatg gagtttaaga tatagtgtat tttcccaagg tttgaactag     2580

ctcttcattt ctttatgttt taaatgcact gacctcccac attccctttt tagtaaaata     2640

ttcagaaata atttaaatac atcattgcaa tgaaaataaa tgttttttat taggcagaat     2700

ccagatgctc aaggcccttc ataatatccc ccagtttagt agttggactt agggaacaaa     2760

ggaaccttta atagaaattg gacagcaaga aagcgagctt agtgatactt gtgggccagg     2820

gcattagcca caccagccac cactttctga taggcagcct gcactggtgg ggtgaattct     2880

ttgccaaagt gatgggccag cacacagacc agcacgttgc ccaggagctg tgggaggaag     2940

ataagaggta tgaacatgat tagcaaaagg gcctagcttg gactcagaat aatccagcct     3000

tatcccaacc ataaaataaa agcagaatgg tagctggatt gtagctgcta ttagcaatat     3060

gaaacctctt acatcagtta caatttatat gcagaaatac cctgttactt ctccccttcc     3120

tatgacatga acttaaccat agaaaagaag gggaaagaaa acatcaaggg tcccatagac     3180

tcaccctgaa gttctcagga tccacgtgca gcttgtcaca gtgcagctca ctcagctggg     3240

caaaggtgcc cttgaggttg tccaggtgag ccaggccatc actaaaggca ccgagcactt     3300

tcttgccatg agccttcacc ttagggttgc ccataacagc atcaggagtg gacagatccc     3360

caaaggactc aaagaacctc tgggtccaag ggtagaccac cagcagccta agggtgggaa     3420

aatagaccaa taggcagaga gagtcagtgc ctatcagaaa cccaagagtc ttctctgtct     3480

ccacatgccc agtttctatt ggtctcctta aacctgtctt gtaaccttga taccaacctg     3540

cccagggcct caccaccaac ggcatccacg ttcaccttgt cccagagagc ggtcacagcg     3600

gacttctcct caggagtcag gtgcaccatg gtgtctgttt gaggttgcta gtgaacacag     3660

ttgtgtcaga agcaaatgta agcaatagat ggctctgccc tgacttttat gcccagccct     3720

ggctcctgcc ctccctgctc ctgggagtag attggccaac cctagggtgt ggctccacag     3780

ggtgaggtct aagtgatgac agccgtacct gtccttggct cttctggcac tggcttagga     3840

gttggacttc aaaccctcag ccctccctct aagatatatc tcttggcccc ataccatcag     3900

tacaaattgc tactaaaaac atcctccttt gcaagtgtat ttacacggta tcgataagct     3960

tgatatcgaa ttcctgcagc ccccttttgc cacctagctg tccaggggtg ccttaaaatg     4020

gcaaacaagg tttgttttct tttcctgttt tcatgccttc ctcttccata tccttgtttc     4080

atattaatac atgtgtatag atcctaaaaa tctatacaca tgtattaata aagcctgatt     4140

ctgccgcttc taggtataga ggccacctgc aagataaata tttgattcac aataactaat     4200

cattctatgg caattgataa caacaaatat atatatatat atatatacgt atatgtgtat     4260

atatatatat atatattcag gaaataatat attctagaat atgtcacatt ctgtctcagg     4320

catccatttt ctttatgatg ccgtttgagg tggagtttta gtcaggtggt cagcttctcc     4380

ttttttttgc catctgccct gtaagcatcc tgctggggac ccagatagga gtcatcactc     4440

taggctgaga acatctgggc acacacccta agcctcagca tgactcatca tgactcagca     4500

ttgctgtgct tgagccagaa ggtttgctta gaaggttaca cagaaccaga aggcgggggt     4560

ggggcactga ccccgacagg ggcctggcca gaactgctca tgcttggact atgggaggtc     4620

actaatggag acacacagaa atgtaacagg aactaaggaa aaactgaagc ttatttaatc     4680

agagatgagg atgctggaag ggatagaggg agctgagctt gtaaaaagta tagtaatcat     4740

tcagcaaatg gttttgaagc acctgctgga tgctaaacac tattttcagt gcttgaatca     4800

taaataagaa taaaacatgt atcttattcc ccacaagagt ccaagtaaaa aataacagtt     4860

aattataatg tgctctgtcc cccaggctgg agtgcagtgg cacgatctca gctcactgca     4920

acctccgcct cccgggttca agcaattctc ctgcctcagc caccctaata gctgggatta     4980

caggtgcaca ccaccatgcc aggctaattt ttgtactttt tgtagaggca gggtatcacc     5040

atgttgtcca agatggtctt gaactcctga gctccaagca gtccacccac ctcagcctcc     5100

caaagtgctg ggattacagg tgtgagacac catgcccaga ttttccatat ttaatagagg     5160

tatttatggg atgggggaaa agaatgtttc tctcactgtg gattatttta gagagtggag     5220

aatggtcaag atttttttaa aaattaagaa aacataagtt ggaccttgag aaatgaaaat     5280

ttattttttt gttggaggat acccattctc tatctcccat cagggcaagc tgtaaggaac     5340

tggctaagac acagtgagac agagtgactt agtcttagag gccccactgg tacgacggtc     5400

accaagcttt cattaaaaaa agtctaacca gctgcattcg actttgactg cagcagctgg     5460

ttagaaggtt ctactggagg agggtcccag cccattgcta aattaacatc aggctctgag     5520

actggcagta tatctctaac agtggttgat gctatcttct ggaacttgcc tgctacattg     5580

agaccactga cccatacata ggaagcccat agctctgtcc tgaactgtta ggccactggt     5640

ccagagagtg tgcatctcct ttgatcctca taataaccct atgagataga cacaattatt     5700

actcttactt tatagatgat gatcctgaaa acataggagt caaggcactt gcccctagct     5760

gggggtatag gggagcagtc ccatgtagta gtagaatgaa aaatgctgct atgctgtgcc     5820

tcccccacct ttcccatgtc tgccctctac tcatggtcta tctctcctgg ctcctgggag     5880

tcatggactc cacccagcac caccaacctg acctaaccac ctatctgagc ctgccagcct     5940

ataacccatc tgggccctga tagctggtgg ccagccctga ccccacccca ccctccctgg     6000

aacctctgat agacacatct ggcacaccag ctcgcaaagt caccgtgagg gtcttgtgtt     6060

tgctgagtca aaattccttg aaatccaagt ccttagagac tcctgctccc aaatttacag     6120

tcatagactt cttcatggct gtctccttta tccacagaat gattcctttg cttcattgcc     6180

ccatccatct gatcctcctc atcagtgcag cacagggccc atgagcagta gctgcagagt     6240

ctcacatagg tctggcactg cctctgacat gtccgacctt aggcaaatgc ttgactcttc     6300

tgagctcagt cttgtcatgg caaaataaag ataataatag tgttttttta tggagttagc     6360

gtgaggatgg aaaacaatag caaaattgat tagactataa aaggtctcaa caaatagtag     6420

tagattttat catccattaa tccttccctc tcctctctta ctcatcccat cacgtatgcc     6480

tcttaatttt cccttaccta taataagagt tattcctctt attatattct tcttatagtg     6540

attctggata ttaaagtggg aatgaggggc aggccactaa cgaagaagat gtttctcaaa     6600

gaagcggggg atccgtcgac aggtcgggca ggaagagggc ctatttccca tgattccttc     6660

atatttgcat atacgataca aggctgttag agagataatt agaattaatt tgactgtaaa     6720

cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc     6780

agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt     6840

cgatttcttg gctttatata tcttgtggaa aggacgaaac accgtctgcc gttactgccc     6900

tgtgtttcag agctatgctg gaaacagcat agcaagttga aataaggcta gtccgttatc     6960

aacttgaaaa agtggcaccg agtcggtgct ttttttgtcg acactagttc tagagcggcc     7020

aaatggcggc cgtaccttta agaccaatga cttacaaggc agctgtagat cttagccact     7080

ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc     7140

tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct     7200

aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt     7260

gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt     7320

ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa     7380

agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat     7440

aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg     7500

gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac     7560

tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact     7620

aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta     7680

gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat     7740

tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc     7800

caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc     7860

cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt     7920

agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc     7980

agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc     8040

tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg     8100

cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga     8160

tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc     8220

caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg     8280

ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt     8340

aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc     8400

ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct     8460

gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg     8520

cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg     8580

tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc     8640

tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca     8700

cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac     8760

tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa     8820

agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg     8880

ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt     8940

ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg     9000

aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc     9060

gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga     9120

tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta     9180

ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc     9240

cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg     9300

atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt     9360

cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa     9420

ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt     9480

cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt     9540

ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt     9600

tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga     9660

taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag     9720

caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata     9780

agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg     9840

gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga     9900

gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca     9960

ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa    10020

acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt    10080

tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac    10140

ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt    10200

ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga    10260

ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc    10320

tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag    10380

cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt    10440

tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca    10500

caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac    10560

aaaagctgga gctgcaagct tgg                                            10583


