
                                SEQUENCE LISTING

<110> Goldfless, Stephen Jacob
      Briggs, Adrian Wrangham
      Chari, Rajagopal
      JIang, Yue
      Hause, Ronald
      Vigneault, Francois

<120> HIGH-THROUGHPUT POLYNUCLEOTIDE LIBRARY
  SEQUENCING AND TRANSCRIPTOME ANALYSIS
  

<130> 735042011740

<140> Not Yet Assigned
<141> Concurrently Herewith

<150> US 62/511,949       
<151> 2017-05-26  

<160> 100

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 27
<212> DNA
<213> Artificial Sequence

<220> 
<223> 5'biotin oligo-dT anchored reverse transcription
      primer

<220> 
<221> misc_feature   
<222> 1
<223> 5' biotin modification with an 18-carbon spacer

<220> 
<221> misc_feature   
<222> 27
<223> n = A,T,C or G

<400> 1
tttttttttt tttttttttt tttttvn                                     27

<210> 2
<211> 78
<212> DNA
<213> Artificial Sequence

<220> 
<223> vessel barcode template oligo

<220> 
<221> misc_feature   
<222> 30, 31, 32, 33, 35, 36, 37, 38, 40, 41, 42, 43
<223> n = A,T,C or G

<400> 2
atccatccac gactgacgga cgtattaaan nnnwnnnnwn nnnagatcgg aagagcacac 60
gtctgaactc cagtcacc                                               78

<210> 3
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<223> template switch oligo

<220> 
<221> misc_feature   
<222> 33
<223> riboguanosine

<220> 
<221> misc_feature   
<222> 34
<223> riboguanosine

<220> 
<221> misc_feature   
<222> 24, 25, 27, 28, 30, 31
<223> n = A,T,C or G

<400> 3
aatacgtccg tcagtcgtgg atgnntnnan ntggg                            35

<210> 4
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> vessel barcode forward primer/universal adaptor
      oligonucleotide

<400> 4
catccacgac tgacggacgt att                                         23

<210> 5
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> vessel barcode reverse primer

<400> 5
gtgactggag ttcagacgtg tgct                                        24

<210> 6
<211> 87
<212> DNA
<213> Artificial Sequence

<220> 
<223> vessel barcode oligo 1

<220> 
<221> misc_feature   
<222> (1)...(5)
<223> phosphorothioate linkage

<220> 
<221> misc_feature   
<222> (83)...(87)
<223> phosphorothioate linkage

<220> 
<221> misc_feature   
<222> 41, 42, 43, 44, 46, 47, 48, 49, 51, 52, 53, 54
<223> n = A,T,C or G

<400> 6
tacgtctacg cgctgctctg ccacgactga cggacgtatt nnnnwnnnnw nnnnagatcg 60
gaagagcaca cgtctgaact ccagtca                                     87

<210> 7
<211> 88
<212> DNA
<213> Artificial Sequence

<220> 
<223> vessel barcode oligo 2

<220> 
<221> misc_feature   
<222> (1)...(5)
<223> phosphorothioate linkage

<220> 
<221> misc_feature   
<222> (84)...(88)
<223> phosphorothioate linkage

<220> 
<221> misc_feature   
<222> 42, 43, 44, 45, 47, 48, 49, 50, 52, 53, 54, 55
<223> n = A,T,C or G

<400> 7
tacgtctacg cgctgctctg ccacgactga cggacgtatt wnnnnwnnnn wnnnnagatc 60
ggaagagcac acgtctgaac tccagtca                                    88

<210> 8
<211> 89
<212> DNA
<213> Artificial Sequence

<220> 
<223> vessel barcode oligo 3

<220> 
<221> misc_feature   
<222> (1)...(5)
<223> phosphorothioate linkage

<220> 
<221> misc_feature   
<222> (85)...(89)
<223> phosphorothioate linkage

<220> 
<221> misc_feature   
<222> 41, 43, 44, 45, 46, 48, 49, 50, 51, 53, 54, 55, 56
<223> n = A,T,C or G

<400> 8
tacgtctacg cgctgctctg ccacgactga cggacgtatt nwnnnnwnnn nwnnnnagat 60
cggaagagca cacgtctgaa ctccagtca                                   89

<210> 9
<211> 90
<212> DNA
<213> Artificial Sequence

<220> 
<223> vessel barcode oligo 4

<220> 
<221> misc_feature   
<222> (1)...(5)
<223> phosphorothioate

<220> 
<221> misc_feature   
<222> (86)...(90)
<223> phosphorothioate

<220> 
<221> misc_feature   
<222> 41, 42, 44, 45, 46, 47, 49, 50, 51, 52, 54, 55, 56, 57
<223> n = A,T,C or G

<400> 9
tacgtctacg cgctgctctg ccacgactga cggacgtatt nnwnnnnwnn nnwnnnnaga 60
tcggaagagc acacgtctga actccagtca                                  90

<210> 10
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> vessel barcode forward primer

<400> 10
gtgactggag ttcagacgtg tgct                                        24

<210> 11
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> vessel barcode reverse primer

<400> 11
tacgtctacg cgctgctctg                                             20

<210> 12
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgG constant RT primer

<220> 
<221> modified_base  
<222> 10
<223> gm

<220> 
<221> modified_base  
<222> 11
<223> gm

<220> 
<221> modified_base  
<222> 12
<223> gm

<400> 12
aggacagccg ggaaggtgt                                              19

<210> 13
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgL constant RT primer
      

<220> 
<221> modified_base  
<222> 9
<223> gm

<220> 
<221> modified_base  
<222> 11
<223> 2'-O-methyl-adenosine

<220> 
<221> modified_base  
<222> 12
<223> gm

<400> 13
gctcccgggt agaagtca                                               18

<210> 14
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgK constant RT primer

<220> 
<221> modified_base  
<222> 11
<223> gm

<220> 
<221> modified_base  
<222> 12
<223> gm

<220> 
<221> modified_base  
<222> 13
<223> 2'-O-methyl-adenosine

<400> 14
ggcctctctg ggatagaagt                                             20

<210> 15
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgM constant RT primer

<220> 
<221> modified_base  
<222> 13
<223> gm

<220> 
<221> modified_base  
<222> 14
<223> cm

<220> 
<221> modified_base  
<222> 15
<223> gm

<400> 15
tgtgaggtgg ctgcgtactt g                                           21

<210> 16
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgA constant RT primer

<220> 
<221> modified_base  
<222> 12
<223> gm

<220> 
<221> modified_base  
<222> 13
<223> gm

<220> 
<221> modified_base  
<222> 14
<223> 2'-O-methyladenosine

<400> 16
ctggctrggt gggaagtttc t                                           21

<210> 17
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgD constant RT primer

<220> 
<221> modified_base  
<222> 12
<223> 2'-O-methyladenosine

<220> 
<221> modified_base  
<222> 13
<223> gmcm

<220> 
<221> modified_base  
<222> 15
<223> cm

<400> 17
cacgcatttg tactcgcctt g                                           21

<210> 18
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgE constant RT primer

<220> 
<221> modified_base  
<222> 10
<223> 2'-O-methyladenosine

<220> 
<221> modified_base  
<222> 12
<223> 2'-O-methyladenosine

<220> 
<221> modified_base  
<222> 13
<223> gm

<400> 18
gatggtggca tagtgaccag                                             20

<210> 19
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRA constant RT primer

<220> 
<221> modified_base  
<222> 15
<223> 2'-O-methyladenosine

<220> 
<221> modified_base  
<222> 16
<223> 2'-O-methyladenosine

<220> 
<221> modified_base  
<222> 18
<223> cm

<400> 19
tgtttgagaa tcaaaatcgg tgaa                                        24

<210> 20
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRB constant RT primer

<220> 
<221> modified_base  
<222> 9
<223> gm

<220> 
<221> modified_base  
<222> 10
<223> gm

<220> 
<221> modified_base  
<222> 11
<223> gm

<400> 20
acgtggtcgg ggaagaag                                               18

<210> 21
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRG constant RT primer

<220> 
<221> modified_base  
<222> 13
<223> ac4cgm

<220> 
<221> modified_base  
<222> 14
<223> gm

<220> 
<221> modified_base  
<222> 16
<223> 2'-O-methyladenosine

<400> 21
caagaagaca aaggtatgtt cc                                          22

<210> 22
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRD constant RT primer

<220> 
<221> modified_base  
<222> 12
<223> gm

<220> 
<221> modified_base  
<222> 13
<223> 2'-O-methyladenosine

<220> 
<221> modified_base  
<222> 14
<223> cm

<400> 22
tcttcttgga tgacacgaga                                             20

<210> 23
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> template switch oligo

<220> 
<221> modified_base  
<222> 24
<223> 2'-deoxyuridine

<220> 
<221> misc_feature   
<222> 34
<223> riboguanosine

<220> 
<221> misc_feature   
<222> 35
<223> riboguanosine

<220> 
<221> modified_base  
<222> 36
<223> 3-deoxyguanosine


<220> 
<221> misc_feature   
<222> 25, 26, 28, 29, 31, 32
<223> n = A,T,C or G

<400> 23
aatacgtccg tcagtcgtgg atgunntnna nntggg                           36

<210> 24
<211> 6
<212> DNA
<213> Artificial Sequence

<220> 
<223> degenerate overhang

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6
<223> n = A,T,C or G

<400> 24
nnnnnn                                                            6

<210> 25
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> short P5 priming sequence

<220> 
<221> modified_base  
<222> 1
<223> phosphorylated adenosine

<220> 
<221> modified_base  
<222> 20
<223> 3' amnino modified (3AmMO) thymine

<400> 25
agatcggaag agcgtcgtgt                                             20

<210> 26
<211> 26
<212> DNA
<213> Artificial Sequence

<220> 
<223> splint oligonucleotide

<220> 
<221> modified_base  
<222> 26
<223> 3'amino modified (3AmMO) nucleotide (a, g, c, or
      t)

<220> 
<221> misc_feature   
<222> 21, 22, 23, 24, 25, 26
<223> n = A,T,C or G

<400> 26
acacgacgct cttccgatct nnnnnn                                      26

<210> 27
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> short P5 reverse primer

<400> 27
acacgacgct cttccgatct                                             20

<210> 28
<211> 64
<212> DNA
<213> Artificial Sequence

<220> 
<223> C7-index-P7 forward primer
      

<220> 
<221> misc_feature   
<222> 25, 26, 27, 28, 29, 30
<223> n = A,T,C or G

<400> 28
caagcagaag acggcatacg agatnnnnnn gtgactggag ttcagacgtg tgctcttccg 60
atct                                                              64

<210> 29
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgG constant reverse primer sequence
      

<400> 29
aagtagtcct tgaccaggca gc                                          22

<210> 30
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgL constant reverse primer sequence
      

<400> 30
ggcttgaagc tcctcagagg a                                           21

<210> 31
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgK constant reverse primer sequence
      

<400> 31
aggcacacaa cagaggcagt tc                                          22

<210> 32
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgM constant reverse primer sequence
      

<400> 32
cgacggggaa ttctcacagg ag                                          22

<210> 33
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgD constant reverse primer sequence
      

<400> 33
tgtctgcacc ctgatatgat gg                                          22

<210> 34
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgA constant 1 reverse primer sequence
      

<400> 34
gggtgctgca gaggctcag                                              19

<210> 35
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgA constant 2 reverse primer sequence
      

<400> 35
gggtgctgtc gaggctcag                                              19

<210> 36
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgE constant reverse primer sequence
      

<400> 36
ggaatgtttt tgcagcagcg gg                                          22

<210> 37
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRA constant reverse primer sequence
      

<400> 37
agtctctcag ctggtacacg g                                           21

<210> 38
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRB constant reverse primer sequence
      

<400> 38
atggctcaaa cacagcgacc tc                                          22

<210> 39
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> C7
      

<400> 39
caagcagaag acggcatacg agat                                        24

<210> 40
<211> 38
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgG adapter-tagged target-specific reverse primer
      sequence

<400> 40
acacgacgct cttccgatct ccagggggaa gacsgatg                         38

<210> 41
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgG adapter-tagged target-specific reverse primer
      sequence

<220> 
<221> misc_feature   
<222> 21
<223> n = A,T,C or G

<400> 41
acacgacgct cttccgatct nccaggggga agacsgatg                        39

<210> 42
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgG adapter-tagged target-specific reverse primer
      sequence

<220> 
<221> misc_feature   
<222> 21, 22
<223> n = A,T,C or G

<400> 42
acacgacgct cttccgatct nnccaggggg aagacsgatg                       40

<210> 43
<211> 41
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgG adapter-tagged target-specific reverse primer
      sequence

<220> 
<221> misc_feature   
<222> 21, 22, 23
<223> n = A,T,C or G

<400> 43
acacgacgct cttccgatct nnnccagggg gaagacsgat g                     41

<210> 44
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgA adapter-tagged target-specific reverse primer
      sequence

<400> 44
acacgacgct cttccgatct ggctcagcgg gaagaccttg                       40

<210> 45
<211> 41
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgA adapter-tagged target-specific reverse primer
      sequence

<220> 
<221> misc_feature   
<222> 21
<223> n = A,T,C or G

<400> 45
acacgacgct cttccgatct nggctcagcg ggaagacctt g                     41

<210> 46
<211> 42
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgA adapter-tagged target-specific reverse primer
      sequence

<220> 
<221> misc_feature   
<222> 21, 22
<223> n = A,T,C or G

<400> 46
acacgacgct cttccgatct nnggctcagc gggaagacct tg                    42

<210> 47
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgA adapter-tagged target-specific reverse primer
      sequence

<220> 
<221> misc_feature   
<222> 21, 22, 23
<223> n = A,T,C or G

<400> 47
acacgacgct cttccgatct nnnggctcag cgggaagacc ttg                   43

<210> 48
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgE adapter-tagged target-specific reverse primer
      sequence

<400> 48
acacgacgct cttccgatct gggaagacgg atgggctctg                       40

<210> 49
<211> 41
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgE adapter-tagged target-specific reverse primer
      sequence

<220> 
<221> misc_feature   
<222> 21
<223> n = A,T,C or G

<400> 49
acacgacgct cttccgatct ngggaagacg gatgggctct g                     41

<210> 50
<211> 42
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgE adapter-tagged target-specific reverse primer
      sequence

<220> 
<221> misc_feature   
<222> 21, 22
<223> n = A,T,C or G

<400> 50
acacgacgct cttccgatct nngggaagac ggatgggctc tg                    42

<210> 51
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgE adapter-tagged target-specific reverse primer
      sequence

<220> 
<221> misc_feature   
<222> 21, 22, 23
<223> n = A,T,C or G

<400> 51
acacgacgct cttccgatct nnngggaaga cggatgggct ctg                   43

<210> 52
<211> 42
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgM adapter-tagged target-specific reverse primer
      sequence

<400> 52
acacgacgct cttccgatct gagacgaggt ggaaaagggt tg                    42

<210> 53
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgM adapter-tagged target-specific reverse primer
      sequence

<220> 
<221> misc_feature   
<222> 21
<223> n = A,T,C or G

<400> 53
acacgacgct cttccgatct ngagacgagg tggaaaaggg ttg                   43

<210> 54
<211> 44
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgM adapter-tagged target-specific reverse primer
      sequence

<220> 
<221> misc_feature   
<222> 21, 22
<223> n = A,T,C or G

<400> 54
acacgacgct cttccgatct nngagacgag gtggaaaagg gttg                  44

<210> 55
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgM adapter-tagged target-specific reverse primer
      sequence

<220> 
<221> misc_feature   
<222> 21, 22, 23
<223> n = A,T,C or G

<400> 55
acacgacgct cttccgatct nnngagacga ggtggaaaag ggttg                 45

<210> 56
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgD adapter-tagged target-specific reverse primer
      sequence

<400> 56
acacgacgct cttccgatct ggaacacatc cggagccttg                       40

<210> 57
<211> 41
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgD adapter-tagged target-specific reverse primer
      sequence

<220> 
<221> misc_feature   
<222> 21
<223> n = A,T,C or G

<400> 57
acacgacgct cttccgatct nggaacacat ccggagcctt g                     41

<210> 58
<211> 42
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgD adapter-tagged target-specific reverse primer
      sequence

<220> 
<221> misc_feature   
<222> 21, 22
<223> n = A,T,C or G

<400> 58
acacgacgct cttccgatct nnggaacaca tccggagcct tg                    42

<210> 59
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgD adapter-tagged target-specific reverse primer
      sequence

<220> 
<221> misc_feature   
<222> 21, 22, 23
<223> n = A,T,C or G

<400> 59
acacgacgct cttccgatct nnnggaacac atccggagcc ttg                   43

<210> 60
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgL adapter-tagged target-specific reverse primer
      sequence

<400> 60
acacgacgct cttccgatct agggygggaa cagagtgac                        39

<210> 61
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgL adapter-tagged target-specific reverse primer
      sequence

<220> 
<221> misc_feature   
<222> 21
<223> n = A,T,C or G

<400> 61
acacgacgct cttccgatct nagggyggga acagagtgac                       40

<210> 62
<211> 41
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgL adapter-tagged target-specific reverse primer
      sequence

<220> 
<221> misc_feature   
<222> 21, 22
<223> n = A,T,C or G

<400> 62
acacgacgct cttccgatct nnagggyggg aacagagtga c                     41

<210> 63
<211> 42
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgL adapter-tagged target-specific reverse primer
      sequence

<220> 
<221> misc_feature   
<222> 21, 22, 23
<223> n = A,T,C or G

<400> 63
acacgacgct cttccgatct nnnagggygg gaacagagtg ac                    42

<210> 64
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgK adapter-tagged target-specific reverse primer
      sequence

<400> 64
acacgacgct cttccgatct gacagatggt gcagccacag                       40

<210> 65
<211> 41
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgK adapter-tagged target-specific reverse primer
      sequence

<220> 
<221> misc_feature   
<222> 21
<223> n = A,T,C or G

<400> 65
acacgacgct cttccgatct ngacagatgg tgcagccaca g                     41

<210> 66
<211> 42
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgK adapter-tagged target-specific reverse primer
      sequence

<220> 
<221> misc_feature   
<222> 21, 22
<223> n = A,T,C or G

<400> 66
acacgacgct cttccgatct nngacagatg gtgcagccac ag                    42

<210> 67
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgK adapter-tagged target-specific reverse primer
      sequence

<220> 
<221> misc_feature   
<222> 21, 22, 23
<223> n = A,T,C or G

<400> 67
acacgacgct cttccgatct nnngacagat ggtgcagcca cag                   43

<210> 68
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRA adapter-tagged target-specific reverse primer
      sequence

<400> 68
acacgacgct cttccgatct cacggcaggg tcagggttc                        39

<210> 69
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRA adapter-tagged target-specific reverse primer
      sequence

<220> 
<221> misc_feature   
<222> 21
<223> n = A,T,C or G

<400> 69
acacgacgct cttccgatct ncacggcagg gtcagggttc                       40

<210> 70
<211> 41
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRA adapter-tagged target-specific reverse primer
      sequence

<220> 
<221> misc_feature   
<222> 21, 22
<223> n = A,T,C or G

<400> 70
acacgacgct cttccgatct nncacggcag ggtcagggtt c                     41

<210> 71
<211> 42
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRA adapter-tagged target-specific reverse primer
      sequence

<220> 
<221> misc_feature   
<222> 21, 22, 23
<223> n = A,T,C or G

<400> 71
acacgacgct cttccgatct nnncacggca gggtcagggt tc                    42

<210> 72
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRB adapter-tagged target-specific reverse primer
      sequence

<400> 72
acacgacgct cttccgatct cgacctcggg tgggaacac                        39

<210> 73
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRB adapter-tagged target-specific reverse primer
      sequence

<220> 
<221> misc_feature   
<222> 21
<223> n = A,T,C or G

<400> 73
acacgacgct cttccgatct ncgacctcgg gtgggaacac                       40

<210> 74
<211> 41
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRB adapter-tagged target-specific reverse primer
      sequence

<220> 
<221> misc_feature   
<222> 21, 22
<223> n = A,T,C or G

<400> 74
acacgacgct cttccgatct nncgacctcg ggtgggaaca c                     41

<210> 75
<211> 42
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRB adapter-tagged target-specific reverse primer
      sequence

<220> 
<221> misc_feature   
<222> 21, 22, 23
<223> n = A,T,C or G

<400> 75
acacgacgct cttccgatct nnncgacctc gggtgggaac ac                    42

<210> 76
<211> 53
<212> DNA
<213> Artificial Sequence

<220> 
<223> C5-P5 reverse primer

<400> 76
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct tcc        53

<210> 77
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<223> P7 priming site (C7)

<400> 77
agatcggaag agcacacgtc tgaactcca                                   29

<210> 78
<211> 58
<212> DNA
<213> Artificial Sequence

<220> 
<223> P5 priming site

<400> 78
agatcggaag agcgtcgtgt agggaaagag tgtagatctc ggtggtcgcc gtatcatt   58

<210> 79
<211> 26
<212> DNA
<213> Artificial Sequence

<220> 
<223> Universal adaptor

<220> 
<221> misc_feature   
<222> 21, 22, 23, 24, 25, 26
<223> n = A,T,C or G

<400> 79
acacgacgct cttccgatct nnnnnn                                      26

<210> 80
<211> 14
<212> DNA
<213> Artificial Sequence

<220> 
<223> Universal adaptor

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 6, 7, 8, 9, 11, 12, 13, 14
<223> n = A,T,C or G

<400> 80
nnnnwnnnnw nnnn                                                   14

<210> 81
<211> 15
<212> DNA
<213> Artificial Sequence

<220> 
<223> Universal adaptor

<220> 
<221> misc_feature   
<222> 2, 3, 4, 5, 7, 8, 9, 10, 12, 13, 14, 15
<223> n = A,T,C or G

<400> 81
wnnnnwnnnn wnnnn                                                  15

<210> 82
<211> 15
<212> DNA
<213> Artificial Sequence

<220> 
<223> Universal adaptor

<220> 
<221> misc_feature   
<222> 1, 3, 4, 5, 7, 8, 9, 10, 12, 13, 14, 15
<223> n = A,T,C or G

<400> 82
nwnnnwnnnn wnnnn                                                  15

<210> 83
<211> 17
<212> DNA
<213> Artificial Sequence

<220> 
<223> Universal adaptor

<220> 
<221> misc_feature   
<222> 1, 2, 4, 5, 6, 7, 9, 10, 11, 12, 14, 15, 16, 17
<223> n = A,T,C or G

<400> 83
nnwnnnnwnn nnwnnnn                                                17

<210> 84
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgM-RT primer

<220> 
<221> modified_base  
<222> 1
<223> biotinylated thymine

<400> 84
tgtgaggtgg ctgcgtactt g                                           21

<210> 85
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgG-RT primer

<220> 
<221> modified_base  
<222> 1
<223> biotinylated adenine

<400> 85
aggacagccg ggaaggtgt                                              19

<210> 86
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgD-RT primer

<220> 
<221> modified_base  
<222> 1
<223> biotinylated cytosine

<400> 86
cacgcatttg tactcgcctt g                                           21

<210> 87
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgA-RT primer

<220> 
<221> modified_base  
<222> 1
<223> biotinylated cytosine

<400> 87
ctggctrggt gggaagtttc t                                           21

<210> 88
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgE-RT primer

<220> 
<221> modified_base  
<222> 1
<223> biotinylated guanine

<400> 88
ggtggcatag tgaccagaga                                             20

<210> 89
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgK-RT primer

<220> 
<221> modified_base  
<222> 1
<223> biotinylated thymine

<400> 89
tattcagcag gcacacaaca ga                                          22

<210> 90
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgL-RT primer

<220> 
<221> modified_base  
<222> 1
<223> biotinylated adenine

<400> 90
agtgtggcct tgttggcttg                                             20

<210> 91
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> TCR-A-RT primer

<220> 
<221> modified_base  
<222> 1
<223> biotinylated guanine

<400> 91
gggagatctc tgcttctgat g                                           21

<210> 92
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> TCR-B-RT primer

<220> 
<221> modified_base  
<222> 1
<223> biotinylated guanine

<400> 92
ggtgaatagg cagacagact tg                                          22

<210> 93
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> CD4-RT primer

<220> 
<221> modified_base  
<222> 1
<223> biotinylated guanine

<400> 93
ggcagtcaat ccgaacact                                              19

<210> 94
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> CD-8-RT primer

<220> 
<221> modified_base  
<222> 1
<223> biotinylated cytosine

<400> 94
ctacaaagtg ggcccttctg                                             20

<210> 95
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> CD4-nested primer

<400> 95
acacgacgct cttccgatct tgtggccttg ccgagggagg                       40

<210> 96
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> CD8-nested primer

<400> 96
acacgacgct cttccgatct tgcggaatcc cagagggcca                       40

<210> 97
<211> 64
<212> DNA
<213> Artificial Sequence

<220> 
<223> C7-bc-P7 primer

<220> 
<221> misc_feature   
<222> 25, 26, 27, 28, 29, 30
<223> n = A,T,C or G

<400> 97
caagcagaag acggcatacg agatnnnnnn gtgactggag ttcagacgtg tgctcttccg 60
atct                                                              64

<210> 98
<211> 58
<212> DNA
<213> Artificial Sequence

<220> 
<223> C5-P5 primer

<400> 98
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct tccgatct   58

<210> 99
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Vessel barcode sequence

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 6, 7, 8, 9, 11, 12, 13, 14, 16, 17, 18, 19
<223> n = A,T,C or G

<400> 99
nnnnwnnnnw nnnnwnnnnw                                             20

<210> 100
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> Vessel barcode sequence

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 6, 9, 10, 11, 13, 16, 17, 18
<223> n = A,T,C or G

<400> 100
nnnnwnscnn nwnscnnn                                               18
