                         SEQUENCE LISTING

<110>  Helmholtz Zentrum Mnchen Deutsches Forschungszentrum fr 
       Gesundheit und Umwelt (GmbH)
 
<120>  Novel CD73 antibody, preparation and uses thereof

<130>  HEL16015PCT

<150>  LU 100265
<151>  2017-05-23

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  372
<212>  DNA
<213>  Rattus norvegicus


<220>
<221>  V_region
<222>  (1)..(372)
<223>  VH region

<220>
<221>  misc_feature
<222>  (76)..(96)
<223>  DNA sequence encoding the VH complementary determining region 1 
       (H-CDR1)

<220>
<221>  misc_feature
<222>  (154)..(171)
<223>  DNA sequence encoding the VH complementary determining region 2 
       (H-CDR2)

<220>
<221>  misc_feature
<222>  (295)..(339)
<223>  DNA sequence encoding the VH complementary determining region 3 
       (H-CDR3)

<400>  1
gaggtgcagc tggtggagtc tgggggaggc ttagtacagc ctggaaggtc catgaaactc       60

tcctgtgcag cctcaggatt cactttcagt ttttattata tggcctgggt ccgccaggct      120

ccaacgaagg gtctggagtg ggtcgcatcc attagtactg gtggtggtaa cacttactat      180

cgcgactccg tgaagggccg attcactatc tccagagatg atgcaaaaag caccctatac      240

ctgcaaatgg acagtctgag gtctgaggaa acggccactt attactgtgc aagacatggg      300

ggggactact acgggtatag agggggctac tttgattact gggaccaagg agtcatggtc      360

acagtctcct ca                                                          372


<210>  2
<211>  21
<212>  DNA
<213>  Rattus norvegicus


<220>
<221>  misc_feature
<222>  (1)..(21)
<223>  DNA sequence encoding the VH complementary determining region 1 
       (H-CDR1)

<400>  2
ggattcactt tcagttttta t                                                 21


<210>  3
<211>  18
<212>  DNA
<213>  Rattus norvegicus


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  DNA sequence encoding the VH complementary determining region 2 
       (H-CDR2)

<400>  3
agtactggtg gtggtaac                                                     18


<210>  4
<211>  45
<212>  DNA
<213>  Rattus norvegicus


<220>
<221>  misc_feature
<222>  (1)..(45)
<223>  DNA sequence encoding the VH complementary determining region 3 
       (H-CDR3)

<400>  4
catggggggg actactacgg gtatagaggg ggctactttg attac                       45


<210>  5
<211>  321
<212>  DNA
<213>  Rattus norvegicus


<220>
<221>  V_region
<222>  (1)..(321)
<223>  VL region

<220>
<221>  misc_feature
<222>  (70)..(102)
<223>  DNA sequence encoding the VL complementary determining region 1 
       (L-CDR1)

<220>
<221>  misc_feature
<222>  (148)..(168)
<223>  DNA sequence encoding the VL complementary determining region 2 
       (L-CDR2)

<220>
<221>  misc_feature
<222>  (265)..(291)
<223>  DNA sequence encoding the VL complementary determining region 3 
       (L-CDR3)

<400>  5
gacatccaga tgacccagac tccatcctcc atgcctgcat ctctgggaga gagagtcacc       60

atcagttgta gagcaagtca gggtattaac aattatctaa actggtatca gcagaagcca      120

gatggaacga ttaaacccct gatctactac acttccaatt tacaatctgg tgtcccatca      180

aggttcagtg gcagtgggtc tgggacagat tattctctca ccatcagcag cctggagcct      240

gaagattttg caatgtatta ctgccaacag tatgataatt ctccgtacac gtttggagct      300

gggaccaagc tggaactgaa a                                                321


<210>  6
<211>  33
<212>  DNA
<213>  Rattus norvegicus


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  DNA sequence encoding the VL complementary determining region 1 
       (L-CDR1)

<400>  6
agagcaagtc agggtattaa caattatcta aac                                    33


<210>  7
<211>  21
<212>  DNA
<213>  Rattus norvegicus


<220>
<221>  misc_feature
<222>  (1)..(21)
<223>  DNA sequence encoding the VL complementary determining region 2 
       (L-CDR2)

<400>  7
tacacttcca atttacaatc t                                                 21


<210>  8
<211>  27
<212>  DNA
<213>  Rattus norvegicus


<220>
<221>  misc_feature
<222>  (1)..(27)
<223>  DNA sequence encoding the VL complementary determining region 3 
       (L-CDR3)

<400>  8
caacagtatg ataattctcc gtacacg                                           27


<210>  9
<211>  124
<212>  PRT
<213>  Rattus norvegicus


<220>
<221>  MISC_FEATURE
<222>  (1)..(124)
<223>  VH region

<220>
<221>  SITE
<222>  (26)..(32)
<223>  VH complementary determining region 1 (VH CDR1)

<220>
<221>  SITE
<222>  (52)..(57)
<223>  VH complementary determining region 2 (H-CDR2)

<220>
<221>  SITE
<222>  (99)..(113)
<223>  VH complementary determining region 3 (H-CDR3)

<400>  9

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Met Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Phe Tyr 
            20                  25                  30          


Tyr Met Ala Trp Val Arg Gln Ala Pro Thr Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Ser Ile Ser Thr Gly Gly Gly Asn Thr Tyr Tyr Arg Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ala Lys Ser Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Arg Ser Glu Glu Thr Ala Thr Tyr Tyr Cys 
                85                  90                  95      


Ala Arg His Gly Gly Asp Tyr Tyr Gly Tyr Arg Gly Gly Tyr Phe Asp 
            100                 105                 110         


Tyr Trp Asp Gln Gly Val Met Val Thr Val Ser Ser 
        115                 120                 


<210>  10
<211>  7
<212>  PRT
<213>  Rattus norvegicus


<220>
<221>  SITE
<222>  (1)..(7)
<223>  VH complementary determining region 1 (H-CDR1)

<400>  10

Gly Phe Thr Phe Ser Phe Tyr 
1               5           


<210>  11
<211>  6
<212>  PRT
<213>  Rattus norvegicus


<220>
<221>  SITE
<222>  (1)..(6)
<223>  VH complementary determining region 2 (H-CDR2)

<400>  11

Ser Thr Gly Gly Gly Asn 
1               5       


<210>  12
<211>  15
<212>  PRT
<213>  Rattus norvegicus


<220>
<221>  SITE
<222>  (1)..(15)
<223>  VH complementary determining region 3 (H-CDR3)

<400>  12

His Gly Gly Asp Tyr Tyr Gly Tyr Arg Gly Gly Tyr Phe Asp Tyr 
1               5                   10                  15  


<210>  13
<211>  107
<212>  PRT
<213>  Rattus norvegicus


<220>
<221>  MISC_FEATURE
<222>  (1)..(107)
<223>  VL region

<220>
<221>  SITE
<222>  (24)..(34)
<223>  VL complementary determining region 1 (L-CDR1)

<220>
<221>  SITE
<222>  (50)..(56)
<223>  VL complementary determining region 2 (L-CDR2)

<220>
<221>  SITE
<222>  (89)..(97)
<223>  VL complementary determining region 3 (L-CDR3)

<400>  13

Asp Ile Gln Met Thr Gln Thr Pro Ser Ser Met Pro Ala Ser Leu Gly 
1               5                   10                  15      


Glu Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Gly Ile Asn Asn Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Ile Lys Pro Leu Ile 
        35                  40                  45              


Tyr Tyr Thr Ser Asn Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Ser Leu Glu Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Met Tyr Tyr Cys Gln Gln Tyr Asp Asn Ser Pro Tyr 
                85                  90                  95      


Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105         


<210>  14
<211>  11
<212>  PRT
<213>  Rattus norvegicus


<220>
<221>  SITE
<222>  (1)..(11)
<223>  VL complementary determining region 1 (L-CDR1)

<400>  14

Arg Ala Ser Gln Gly Ile Asn Asn Tyr Leu Asn 
1               5                   10      


<210>  15
<211>  7
<212>  PRT
<213>  Rattus norvegicus


<220>
<221>  SITE
<222>  (1)..(7)
<223>  VL complementary determining region 2 (L-CDR2)

<400>  15

Tyr Thr Ser Asn Leu Gln Ser 
1               5           


<210>  16
<211>  9
<212>  PRT
<213>  Rattus norvegicus


<220>
<221>  SITE
<222>  (1)..(9)
<223>  VL complementary determining region 3 (L-CDR3)

<400>  16

Gln Gln Tyr Asp Asn Ser Pro Tyr Thr 
1               5                   


<210>  17
<211>  574
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(574)
<223>  5'-nucleotidase isoform 1 preproprotein, Accession: NP_002517

<400>  17

Met Cys Pro Arg Ala Ala Arg Ala Pro Ala Thr Leu Leu Leu Ala Leu 
1               5                   10                  15      


Gly Ala Val Leu Trp Pro Ala Ala Gly Ala Trp Glu Leu Thr Ile Leu 
            20                  25                  30          


His Thr Asn Asp Val His Ser Arg Leu Glu Gln Thr Ser Glu Asp Ser 
        35                  40                  45              


Ser Lys Cys Val Asn Ala Ser Arg Cys Met Gly Gly Val Ala Arg Leu 
    50                  55                  60                  


Phe Thr Lys Val Gln Gln Ile Arg Arg Ala Glu Pro Asn Val Leu Leu 
65                  70                  75                  80  


Leu Asp Ala Gly Asp Gln Tyr Gln Gly Thr Ile Trp Phe Thr Val Tyr 
                85                  90                  95      


Lys Gly Ala Glu Val Ala His Phe Met Asn Ala Leu Arg Tyr Asp Ala 
            100                 105                 110         


Met Ala Leu Gly Asn His Glu Phe Asp Asn Gly Val Glu Gly Leu Ile 
        115                 120                 125             


Glu Pro Leu Leu Lys Glu Ala Lys Phe Pro Ile Leu Ser Ala Asn Ile 
    130                 135                 140                 


Lys Ala Lys Gly Pro Leu Ala Ser Gln Ile Ser Gly Leu Tyr Leu Pro 
145                 150                 155                 160 


Tyr Lys Val Leu Pro Val Gly Asp Glu Val Val Gly Ile Val Gly Tyr 
                165                 170                 175     


Thr Ser Lys Glu Thr Pro Phe Leu Ser Asn Pro Gly Thr Asn Leu Val 
            180                 185                 190         


Phe Glu Asp Glu Ile Thr Ala Leu Gln Pro Glu Val Asp Lys Leu Lys 
        195                 200                 205             


Thr Leu Asn Val Asn Lys Ile Ile Ala Leu Gly His Ser Gly Phe Glu 
    210                 215                 220                 


Met Asp Lys Leu Ile Ala Gln Lys Val Arg Gly Val Asp Val Val Val 
225                 230                 235                 240 


Gly Gly His Ser Asn Thr Phe Leu Tyr Thr Gly Asn Pro Pro Ser Lys 
                245                 250                 255     


Glu Val Pro Ala Gly Lys Tyr Pro Phe Ile Val Thr Ser Asp Asp Gly 
            260                 265                 270         


Arg Lys Val Pro Val Val Gln Ala Tyr Ala Phe Gly Lys Tyr Leu Gly 
        275                 280                 285             


Tyr Leu Lys Ile Glu Phe Asp Glu Arg Gly Asn Val Ile Ser Ser His 
    290                 295                 300                 


Gly Asn Pro Ile Leu Leu Asn Ser Ser Ile Pro Glu Asp Pro Ser Ile 
305                 310                 315                 320 


Lys Ala Asp Ile Asn Lys Trp Arg Ile Lys Leu Asp Asn Tyr Ser Thr 
                325                 330                 335     


Gln Glu Leu Gly Lys Thr Ile Val Tyr Leu Asp Gly Ser Ser Gln Ser 
            340                 345                 350         


Cys Arg Phe Arg Glu Cys Asn Met Gly Asn Leu Ile Cys Asp Ala Met 
        355                 360                 365             


Ile Asn Asn Asn Leu Arg His Thr Asp Glu Met Phe Trp Asn His Val 
    370                 375                 380                 


Ser Met Cys Ile Leu Asn Gly Gly Gly Ile Arg Ser Pro Ile Asp Glu 
385                 390                 395                 400 


Arg Asn Asn Gly Thr Ile Thr Trp Glu Asn Leu Ala Ala Val Leu Pro 
                405                 410                 415     


Phe Gly Gly Thr Phe Asp Leu Val Gln Leu Lys Gly Ser Thr Leu Lys 
            420                 425                 430         


Lys Ala Phe Glu His Ser Val His Arg Tyr Gly Gln Ser Thr Gly Glu 
        435                 440                 445             


Phe Leu Gln Val Gly Gly Ile His Val Val Tyr Asp Leu Ser Arg Lys 
    450                 455                 460                 


Pro Gly Asp Arg Val Val Lys Leu Asp Val Leu Cys Thr Lys Cys Arg 
465                 470                 475                 480 


Val Pro Ser Tyr Asp Pro Leu Lys Met Asp Glu Val Tyr Lys Val Ile 
                485                 490                 495     


Leu Pro Asn Phe Leu Ala Asn Gly Gly Asp Gly Phe Gln Met Ile Lys 
            500                 505                 510         


Asp Glu Leu Leu Arg His Asp Ser Gly Asp Gln Asp Ile Asn Val Val 
        515                 520                 525             


Ser Thr Tyr Ile Ser Lys Met Lys Val Ile Tyr Pro Ala Val Glu Gly 
    530                 535                 540                 


Arg Ile Lys Phe Ser Thr Gly Ser His Cys His Gly Ser Phe Ser Leu 
545                 550                 555                 560 


Ile Phe Leu Ser Leu Trp Ala Val Ile Phe Val Leu Tyr Gln 
                565                 570                 


<210>  18
<211>  524
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(524)
<223>  5'-nucleotidase isoform 2 preproprotein, Accession: NP_001191742

<400>  18

Met Cys Pro Arg Ala Ala Arg Ala Pro Ala Thr Leu Leu Leu Ala Leu 
1               5                   10                  15      


Gly Ala Val Leu Trp Pro Ala Ala Gly Ala Trp Glu Leu Thr Ile Leu 
            20                  25                  30          


His Thr Asn Asp Val His Ser Arg Leu Glu Gln Thr Ser Glu Asp Ser 
        35                  40                  45              


Ser Lys Cys Val Asn Ala Ser Arg Cys Met Gly Gly Val Ala Arg Leu 
    50                  55                  60                  


Phe Thr Lys Val Gln Gln Ile Arg Arg Ala Glu Pro Asn Val Leu Leu 
65                  70                  75                  80  


Leu Asp Ala Gly Asp Gln Tyr Gln Gly Thr Ile Trp Phe Thr Val Tyr 
                85                  90                  95      


Lys Gly Ala Glu Val Ala His Phe Met Asn Ala Leu Arg Tyr Asp Ala 
            100                 105                 110         


Met Ala Leu Gly Asn His Glu Phe Asp Asn Gly Val Glu Gly Leu Ile 
        115                 120                 125             


Glu Pro Leu Leu Lys Glu Ala Lys Phe Pro Ile Leu Ser Ala Asn Ile 
    130                 135                 140                 


Lys Ala Lys Gly Pro Leu Ala Ser Gln Ile Ser Gly Leu Tyr Leu Pro 
145                 150                 155                 160 


Tyr Lys Val Leu Pro Val Gly Asp Glu Val Val Gly Ile Val Gly Tyr 
                165                 170                 175     


Thr Ser Lys Glu Thr Pro Phe Leu Ser Asn Pro Gly Thr Asn Leu Val 
            180                 185                 190         


Phe Glu Asp Glu Ile Thr Ala Leu Gln Pro Glu Val Asp Lys Leu Lys 
        195                 200                 205             


Thr Leu Asn Val Asn Lys Ile Ile Ala Leu Gly His Ser Gly Phe Glu 
    210                 215                 220                 


Met Asp Lys Leu Ile Ala Gln Lys Val Arg Gly Val Asp Val Val Val 
225                 230                 235                 240 


Gly Gly His Ser Asn Thr Phe Leu Tyr Thr Gly Asn Pro Pro Ser Lys 
                245                 250                 255     


Glu Val Pro Ala Gly Lys Tyr Pro Phe Ile Val Thr Ser Asp Asp Gly 
            260                 265                 270         


Arg Lys Val Pro Val Val Gln Ala Tyr Ala Phe Gly Lys Tyr Leu Gly 
        275                 280                 285             


Tyr Leu Lys Ile Glu Phe Asp Glu Arg Gly Asn Val Ile Ser Ser His 
    290                 295                 300                 


Gly Asn Pro Ile Leu Leu Asn Ser Ser Ile Pro Glu Asp Pro Ser Ile 
305                 310                 315                 320 


Lys Ala Asp Ile Asn Lys Trp Arg Ile Lys Leu Asp Asn Tyr Ser Thr 
                325                 330                 335     


Gln Glu Leu Gly Lys Thr Ile Val Tyr Leu Asp Gly Ser Ser Gln Ser 
            340                 345                 350         


Cys Arg Phe Arg Glu Cys Asn Met Gly Asn Leu Ile Cys Asp Ala Met 
        355                 360                 365             


Ile Asn Asn Asn Leu Arg His Thr Asp Glu Met Phe Trp Asn His Val 
    370                 375                 380                 


Ser Met Cys Ile Leu Asn Gly Gly Gly Ile Arg Ser Pro Ile Asp Glu 
385                 390                 395                 400 


Arg Asn Asn Gly Ile His Val Val Tyr Asp Leu Ser Arg Lys Pro Gly 
                405                 410                 415     


Asp Arg Val Val Lys Leu Asp Val Leu Cys Thr Lys Cys Arg Val Pro 
            420                 425                 430         


Ser Tyr Asp Pro Leu Lys Met Asp Glu Val Tyr Lys Val Ile Leu Pro 
        435                 440                 445             


Asn Phe Leu Ala Asn Gly Gly Asp Gly Phe Gln Met Ile Lys Asp Glu 
    450                 455                 460                 


Leu Leu Arg His Asp Ser Gly Asp Gln Asp Ile Asn Val Val Ser Thr 
465                 470                 475                 480 


Tyr Ile Ser Lys Met Lys Val Ile Tyr Pro Ala Val Glu Gly Arg Ile 
                485                 490                 495     


Lys Phe Ser Thr Gly Ser His Cys His Gly Ser Phe Ser Leu Ile Phe 
            500                 505                 510         


Leu Ser Leu Trp Ala Val Ile Phe Val Leu Tyr Gln 
        515                 520                 


