                         SEQUENCE LISTING

<110>  AGENCY FOR SCIENCE,TECHNOLOGY AND REASEARCH
       Wan, Andrew
       Lim, Tze-Chiun
       Lim, Jia-Kai
 
<120>  Toxicity testing device and methods for making and using the same

<130>  2018.P01109

<150>  SG 10201703946Q
<151>  2017-05-15

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  4701
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  secreted form of embryonic alkaline phosphatase

<400>  1
gcggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat       60

cgtaactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg      120

ctgtccccag tgcaagtgca ggtgccagaa catttctcta tcgaaggatc tgcgatcgct      180

ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag      240

gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg      300

tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag      360

tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag      420

gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt      480

tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg      540

taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta      600

gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt      660

cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacc tgagatcacc      720

ggtcaccatg gttctggggc cctgcatgct gctgctgctg ctgctgctgg gcctgaggct      780

acagctctcc ctgggcatca tcccagttga ggaggagaac ccggacttct ggaaccgcga      840

ggcagccgag gccctgggtg ccgccaagaa gctgcagcct gcacagacag ccgccaagaa      900

cctcatcatc ttcctgggcg atgggatggg ggtgtctacg gtgacagctg ccaggatcct      960

aaaagggcag aagaaggaca aactggggcc tgagataccc ctggctatgg accgcttccc     1020

atatgtggct ctgtccaaga catacaatgt agacaaacat gtgccagaca gtggagccac     1080

agccacggcc tacctgtgcg gggtcaaggg caacttccag accattggct tgagtgcagc     1140

cgcccgcttt aaccagtgca acacgacacg cggcaacgag gtcatctccg tgatgaatcg     1200

ggccaagaaa gcagggaagt cagtgggagt ggtaaccacc acacgagtgc agcacgcctc     1260

gccagccggc acctacgccc acacggtgaa ccgcaactgg tactcggacg ccgacgtgcc     1320

tgcctcggcc cgccaggagg ggtgccagga catcgctacg cagctcatct ccaacatgga     1380

cattgatgtg atcctgggtg gaggccgaaa gtacatgttt cgcatgggaa ccccagaccc     1440

tgagtaccca gatgactaca gccaaggtgg gaccaggctg gacgggaaga atctggtgca     1500

ggaatggctg gcgaagcgcc agggtgcccg gtatgtgtgg aaccgcactg agctcatgca     1560

ggcttccctg gacccgtctg tgacccatct catgggtctc tttgagcctg gagacatgaa     1620

atacgagatc caccgagact ccacactgga cccctccctg atggagatga cagaggctgc     1680

cctgcgcctg ctgagcagga acccccgcgg cttcttcctc ttcgtggagg gtggtcgcat     1740

cgaccacggt catcacgaaa gcagggctta ccgggcactg actgagacga tcatgttcga     1800

cgacgccatt gagagggcgg gccagctcac cagcgaggag gacacgctga gcctcgtcac     1860

tgccgaccac tcccacgtct tctccttcgg aggctacccc ctgcgaggga gctccatctt     1920

cgggctggcc cctggcaagg cccgggacag gaaggcctac acggtcctcc tatacggaaa     1980

cggtccaggc tatgtgctca aggacggcgc ccggccggat gttaccgaga gcgagagcgg     2040

gagccccgag tatcggcagc agtcagcagt gcccctggac gaagagaccc acgcaggcga     2100

ggacgtggcg gtgttcgcgc gcggcccgca ggcgcacctg gttcacggcg tgcaggagca     2160

gaccttcata gcgcacgtca tggccttcgc cgcctgcctg gagccctaca ccgcctgcga     2220

cctggcgccc cccgccggca ccaccgacgc cgcgcacccg gggcggtccc ggtccaagcg     2280

tctggattga agctagctgg ccagacatga taagatacat tgatgagttt ggacaaacca     2340

caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat     2400

ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt     2460

ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg     2520

gtatggaatt ctaaaataca gcatagcaaa actttaacct ccaaatcaag cctctacttg     2580

aatccttttc tgagggatga ataaggcata ggcatcaggg gctgttgcca atgtgcatta     2640

gctgtttgca gcctcacctt ctttcatgga gtttaagata tagtgtattt tcccaaggtt     2700

tgaactagct cttcatttct ttatgtttta aatgcactga cctcccacat tcccttttta     2760

gtaaaatatt cagaaataat ttaaatacat cattgcaatg aaaataaatg ttttttatta     2820

ggcagaatcc agatgctcaa ggcccttcat aatatccccc agtttagtag ttggacttag     2880

ggaacaaagg aacctttaat agaaattgga cagcaagaaa gcgagcttct agcttatcct     2940

cagtcctgct cctctgccac aaagtgcacg cagttgccgg ccgggtcgcg cagggcgaac     3000

tcccgccccc acggctgctc gccgatctcg gtcatggccg gcccggaggc gtcccggaag     3060

ttcgtggaca cgacctccga ccactcggcg tacagctcgt ccaggccgcg cacccacacc     3120

caggccaggg tgttgtccgg caccacctgg tcctggaccg cgctgatgaa cagggtcacg     3180

tcgtcccgga ccacaccggc gaagtcgtcc tccacgaagt cccgggagaa cccgagccgg     3240

tcggtccaga actcgaccgc tccggcgacg tcgcgcgcgg tgagcaccgg aacggcactg     3300

gtcaacttgg ccatgatggc cctcctatag tgagtcgtat tatactatgc cgatatacta     3360

tgccgatgat taattgtcaa aacagcgtgg atggcgtctc cagcttatct gacggttcac     3420

taaacgagct ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa     3480

tggggcggag ttgttacgac attttggaaa gtcccgttga tttactagtc aaaacaaact     3540

cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg     3600

cccattgatg tactgccaaa accgcatcat catggtaata gcgatgacta atacgtagat     3660

gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca     3720

tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc     3780

caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg     3840

cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca     3900

gccaggcggg ccatttaccg taagttatgt aacgcctgca ggttaattaa gaacatgtga     3960

gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat     4020

aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac     4080

ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct     4140

gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg     4200

ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg     4260

ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt     4320

cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg     4380

attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac     4440

ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga     4500

aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt     4560

gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt     4620

tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatggct     4680

agttaattaa catttaaatc a                                               4701


<210>  2
<211>  720
<212>  DNA
<213>  Aequorea victoria

<400>  2
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac       60

ggcgacgtaa acggccacaa gttcagcgtg tctggcgagg gcgagggcga tgccacctac      120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc      180

ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag      240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc      300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg      360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac      420

aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac      480

ggcatcaagg cgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc      540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac      600

tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc      660

ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa      720


<210>  3
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KB recognition site


<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  R is a purine

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  N is any base

<220>
<221>  misc_feature
<222>  (6)..(8)
<223>  Y IS A Pyrimidine

<400>  3
gggrnyyycc                                                              10


<210>  4
<211>  458
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hybrid promoter

<400>  4
gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg       60

tcggcaattg aaccggtagg gaggggagct gtgcacacgg cggaggcgca cggcgtgggc      120

acccagcacc cggtacactg tgtcctcccg ctgcacccag ccccttccgc gccgaggcgt      180

ccccgaggcg caagtgggcc gccttcaggg aactgaccgc ccgcggcccg tgtgcagagc      240

cgggtgcgcc cggcccagtg cgcgcggccg ggtgtttcgc ctggagccgc aagtgtctag      300

acgcggggcg tgtgcaggca gcgcccggcc ggggcggggc ttttgcactc gtcccggctc      360

tttctagcta taaacactgc ttgccgcgct gcactccacc acgcctcctc caagtcccag      420

cgaacccgcg tgcaacctgt cccgactcta gcaagctt                              458


