                         SEQUENCE LISTING

<110>  BASF SE
 
<120>  Process for producing succinic acid

<130>  PF170371PC

<150>  17171894.3
<151>  2017-05-19

<160>  23    

<170>  PatentIn version 3.3

<210>  1
<211>  1517
<212>  DNA
<213>  Basfia succiniciproducens


<220>
<221>  MISC_FEATURE
<223>  16 S rDNA of strain DD1

<400>  1
tttgatcctg gctcagattg aacgctggcg gcaggcttaa cacatgcaag tcgaacggta     60

gcgggaggaa agcttgcttt ctttgccgac gagtggcgga cgggtgagta atgcttgggg    120

atctggctta tggaggggga taacgacggg aaactgtcgc taataccgcg taatatcttc    180

ggattaaagg gtgggacttt cgggccaccc gccataagat gagcccaagt gggattaggt    240

agttggtggg gtaaaggcct accaagccga cgatctctag ctggtctgag aggatgacca    300

gccacactgg aactgagaca cggtccagac tcctacggga ggcagcagtg gggaatattg    360

cacaatgggg ggaaccctga tgcagccatg ccgcgtgaat gaagaaggcc ttcgggttgt    420

aaagttcttt cggtgacgag gaaggtgttt gttttaatag gacaagcaat tgacgttaat    480

cacagaagaa gcaccggcta actccgtgcc agcagccgcg gtaatacgga gggtgcgagc    540

gttaatcgga ataactgggc gtaaagggca tgcaggcgga cttttaagtg agatgtgaaa    600

gccccgggct taacctggga attgcatttc agactgggag tctagagtac tttagggagg    660

ggtagaattc cacgtgtagc ggtgaaatgc gtagagatgt ggaggaatac cgaaggcgaa    720

ggcagcccct tgggaagata ctgacgctca tatgcgaaag cgtggggagc aaacaggatt    780

agataccctg gtagtccacg cggtaaacgc tgtcgatttg gggattgggc tttaggcctg    840

gtgctcgtag ctaacgtgat aaatcgaccg cctggggagt acggccgcaa ggttaaaact    900

caaatgaatt gacgggggcc cgcacaagcg gtggagcatg tggtttaatt cgatgcaacg    960

cgaagaacct tacctactct tgacatccag agaatcctgt agagatacgg gagtgccttc   1020

gggagctctg agacaggtgc tgcatggctg tcgtcagctc gtgttgtgaa atgttgggtt   1080

aagtcccgca acgagcgcaa cccttatcct ttgttgccag catgtaaaga tgggaactca   1140

aaggagactg ccggtgacaa accggaggaa ggtggggatg acgtcaagtc atcatggccc   1200

ttacgagtag ggctacacac gtgctacaat ggtgcataca gagggcggcg ataccgcgag   1260

gtagagcgaa tctcagaaag tgcatcgtag tccggattgg agtctgcaac tcgactccat   1320

gaagtcggaa tcgctagtaa tcgcaaatca gaatgttgcg gtgaatacgt tcccgggcct   1380

tgtacacacc gcccgtcaca ccatgggagt gggttgtacc agaagtagat agcttaacct   1440

tcgggggggg cgtttaccac ggtatgattc atgactgggg tgaagtcgta acaaggtaac   1500

cgtaggggaa cctgcgg                                                  1517


<210>  2
<211>  3008
<212>  DNA
<213>  Basfia succiniciproducens


<220>
<221>  MISC_FEATURE
<223>  23 S rDNA of strain DD1

<400>  2
agtaataacg aacgacacag gtataagaat acttgaggtt gtatggttaa gtgactaagc     60

gtacaaggtg gatgccttgg caatcagagg cgaagaagga cgtgctaatc tgcgaaaagc    120

ttgggtgagt tgataagaag cgtctaaccc aagatatccg aatggggcaa cccagtagat    180

gaagaatcta ctatcaataa ccgaatccat aggttattga ggcaaaccgg gagaactgaa    240

acatctaagt accccgagga aaagaaatca accgagatta cgtcagtagc ggcgagcgaa    300

agcgtaagag ccggcaagtg atagcatgag gattagagga atcggctggg aagccgggcg    360

gcacagggtg atagccccgt acttgaaaat cattgtgtgg tactgagctt gcgagaagta    420

gggcgggaca cgagaaatcc tgtttgaaga aggggggacc atcctccaag gctaaatact    480

cctgattgac cgatagtgaa ccagtactgt gaaggaaagg cgaaaagaac cccggtgagg    540

ggagtgaaat agaacctgaa accttgtacg tacaagcagt gggagcccgc gagggtgact    600

gcgtaccttt tgtataatgg gtcagcgact tatattatgt agcgaggtta accgaatagg    660

ggagccgaag ggaaaccgag tcttaactgg gcgtcgagtt gcatgatata gacccgaaac    720

ccggtgatct agccatgggc aggttgaagg ttgggtaaca ctaactggag gaccgaaccg    780

actaatgttg aaaaattagc ggatgacctg tggctggggg tgaaaggcca atcaaaccgg    840

gagatagctg gttctccccg aaatctattt aggtagagcc ttatgtgaat accttcgggg    900

gtagagcact gtttcggcta gggggccatc ccggcttacc aacccgatgc aaactgcgaa    960

taccgaagag taatgcatag gagacacacg gcgggtgcta acgttcgtcg tggagaggga   1020

aacaacccag accgccagct aaggtcccaa agtttatatt aagtgggaaa cgaagtggga   1080

aggcttagac agctaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag   1140

ctcactagtc gagtcggcct gcgcggaaga tgtaacgggg ctcaaatata gcaccgaagc   1200

tgcggcatca ggcgtaagcc tgttgggtag gggagcgtcg tgtaagcgga agaaggtggt   1260

tcgagagggc tgctggacgt atcacgagtg cgaatgctga cataagtaac gataaaacgg   1320

gtgaaaaacc cgttcgccgg aagaccaagg gttcctgtcc aacgttaatc ggggcagggt   1380

gagtcggccc ctaaggcgag gctgaagagc gtagtcgatg ggaaacgggt taatattccc   1440

gtacttgtta taattgcgat gtggggacgg agtaggttag gttatcgacc tgttggaaaa   1500

ggtcgtttaa gttggtaggt ggagcgttta ggcaaatccg gacgcttatc aacaccgaga   1560

gatgatgacg aggcgctaag gtgccgaagt aaccgatacc acacttccag gaaaagccac   1620

taagcgtcag attataataa accgtactat aaaccgacac aggtggtcag gtagagaata   1680

ctcaggcgct tgagagaact cgggtgaagg aactaggcaa aatagcaccg taacttcggg   1740

agaaggtgcg ccggcgtaga ttgtagaggt atacccttga aggttgaacc ggtcgaagtg   1800

acccgctggc tgcaactgtt tattaaaaac acagcactct gcaaacacga aagtggacgt   1860

atagggtgtg atgcctgccc ggtgctggaa ggttaattga tggcgttatc gcaagagaag   1920

cgcctgatcg aagccccagt aaacggcggc cgtaactata acggtcctaa ggtagcgaaa   1980

ttccttgtcg ggtaagttcc gacctgcacg aatggcataa tgatggccag gctgtctcca   2040

cccgagactc agtgaaattg aaatcgccgt gaagatgcgg tgtacccgcg gctagacgga   2100

aagaccccgt gaacctttac tatagcttga cactgaacct tgaattttga tgtgtaggat   2160

aggtgggagg ctttgaagcg gtaacgccag ttatcgtgga gccatccttg aaataccacc   2220

ctttaacgtt tgatgttcta acgaagtgcc cggaacgggt actcggacag tgtctggtgg   2280

gtagtttgac tggggcggtc tcctcccaaa gagtaacgga ggagcacgaa ggtttgctaa   2340

tgacggtcgg acatcgtcag gttagtgcaa tggtataagc aagcttaact gcgagacgga   2400

caagtcgagc aggtgcgaaa gcaggtcata gtgatccggt ggttctgaat ggaagggcca   2460

tcgctcaacg gataaaaggt actccgggga taacaggctg ataccgccca agagttcata   2520

tcgacggcgg tgtttggcac ctcgatgtcg gctcatcaca tcctggggct gaagtaggtc   2580

ccaagggtat ggctgttcgc catttaaagt ggtacgcgag ctgggtttaa aacgtcgtga   2640

gacagtttgg tccctatctg ccgtgggcgt tggagaattg agaggggctg ctcctagtac   2700

gagaggaccg gagtggacgc atcactggtg ttccggttgt gtcgccagac gcattgccgg   2760

gtagctacat gcggaagaga taagtgctga aagcatctaa gcacgaaact tgcctcgaga   2820

tgagttctcc cagtatttaa tactgtaagg gttgttggag acgacgacgt agataggccg   2880

ggtgtgtaag cgttgcgaga cgttgagcta accggtacta attgcccgag aggcttagcc   2940

atacaacgct caagtgtttt tggtagtgaa agttattacg gaataagtaa gtagtcaggg   3000

aatcggct                                                            3008


<210>  3
<211>  933
<212>  DNA
<213>  Basfia succiniciproducens


<220>
<221>  MISC_FEATURE
<223>  nucleotide sequence of rbsK-gene from strain DD1

<400>  3
atgcatatga caaacaaaat ttgggtatta ggcgatgccg tggtggattt aattcctgac     60

ggagacaacc attatttgcg ttgcgcaggc ggcgcaccgg ctaatgtggc ggtcggcgtt    120

gcccgtttag gtgtgcctag cgcatttatc ggccgtgtag gtaaagatcc gttaggggaa    180

tttatgcgcg atacgctgaa tcaggaaaat gtaaacaccg attatatgtt gttagatcct    240

aaacaacgta cttcgacggt ggtggttgga ttaaccgacg gcgaacgtag ttttaccttt    300

atggtgaatc caagtgcgga tcaattttta caaatttccg atctgccgca atttcaagcc    360

ggagactggt tgcactgctg ctctatcgcc ttaatcaatg aaccgacccg cagcgctact    420

ttcacggcaa tgaaaaatat ccgtgcggcc ggcggtaaag tatctttcga tccgaattta    480

cgcgaaagct tatggaaatc ccaggatgaa atgatcgatg tggtgatgga agcggtaagc    540

cttgccgacg tattgaaatt ttcagaagaa gaattaacgc tgttaaccca taccgacagc    600

ctggaaaaat cttttgaaaa aatcaccgca ctttatcccg ataaattgat tattgtcact    660

ttagggaaag atggcgcgct ctatcatctg cacggtaaaa aagaggtggt tgcagggaaa    720

gcgctgaaac cggtagatac caccggtgcc ggcgacgctt ttgtcagcgg gttattagcc    780

ggattatcac aaacggaaaa ctggcagcaa cctgaacaac tcgttactat tattcgccag    840

gccaacgcca gcggcgcgct tgccacaacg gcaaaaggcg ctatgtcggc attaccgaat    900

cggcaacaat tagcggaatt tttagcaaac taa                                 933


<210>  4
<211>  310
<212>  PRT
<213>  Basfia succiniciproducens


<220>
<221>  SITE
<222>  (1)..(310)
<223>  amino acid sequence of RbsK from strain DD1

<400>  4

Met His Met Thr Asn Lys Ile Trp Val Leu Gly Asp Ala Val Val Asp 
1               5                   10                  15      


Leu Ile Pro Asp Gly Asp Asn His Tyr Leu Arg Cys Ala Gly Gly Ala 
            20                  25                  30          


Pro Ala Asn Val Ala Val Gly Val Ala Arg Leu Gly Val Pro Ser Ala 
        35                  40                  45              


Phe Ile Gly Arg Val Gly Lys Asp Pro Leu Gly Glu Phe Met Arg Asp 
    50                  55                  60                  


Thr Leu Asn Gln Glu Asn Val Asn Thr Asp Tyr Met Leu Leu Asp Pro 
65                  70                  75                  80  


Lys Gln Arg Thr Ser Thr Val Val Val Gly Leu Thr Asp Gly Glu Arg 
                85                  90                  95      


Ser Phe Thr Phe Met Val Asn Pro Ser Ala Asp Gln Phe Leu Gln Ile 
            100                 105                 110         


Ser Asp Leu Pro Gln Phe Gln Ala Gly Asp Trp Leu His Cys Cys Ser 
        115                 120                 125             


Ile Ala Leu Ile Asn Glu Pro Thr Arg Ser Ala Thr Phe Thr Ala Met 
    130                 135                 140                 


Lys Asn Ile Arg Ala Ala Gly Gly Lys Val Ser Phe Asp Pro Asn Leu 
145                 150                 155                 160 


Arg Glu Ser Leu Trp Lys Ser Gln Asp Glu Met Ile Asp Val Val Met 
                165                 170                 175     


Glu Ala Val Ser Leu Ala Asp Val Leu Lys Phe Ser Glu Glu Glu Leu 
            180                 185                 190         


Thr Leu Leu Thr His Thr Asp Ser Leu Glu Lys Ser Phe Glu Lys Ile 
        195                 200                 205             


Thr Ala Leu Tyr Pro Asp Lys Leu Ile Ile Val Thr Leu Gly Lys Asp 
    210                 215                 220                 


Gly Ala Leu Tyr His Leu His Gly Lys Lys Glu Val Val Ala Gly Lys 
225                 230                 235                 240 


Ala Leu Lys Pro Val Asp Thr Thr Gly Ala Gly Asp Ala Phe Val Ser 
                245                 250                 255     


Gly Leu Leu Ala Gly Leu Ser Gln Thr Glu Asn Trp Gln Gln Pro Glu 
            260                 265                 270         


Gln Leu Val Thr Ile Ile Arg Gln Ala Asn Ala Ser Gly Ala Leu Ala 
        275                 280                 285             


Thr Thr Ala Lys Gly Ala Met Ser Ala Leu Pro Asn Arg Gln Gln Leu 
    290                 295                 300                 


Ala Glu Phe Leu Ala Asn 
305                 310 


<210>  5
<211>  1695
<212>  DNA
<213>  Basfia succiniciproducens


<220>
<221>  MISC_FEATURE
<223>  nucleotide sequence of fruA-gene from strain DD1

<400>  5
ttgaaggata agccgatgaa tatttttctt acgcaatcac caaatttagg tcgtgcaaaa     60

gcgtttttat tgcaccaggt tttggctgcc gcagtaaaac aacaaaatca tcaactggta    120

gaaaatgccg aacaagcgga tttagcgatt gttttcggta aaactttgcc gaatttgacc    180

gcacttttag gtaaaaaagt gtatttggcg gatgaagaac aagcgttgaa tgcgcctgaa    240

aataccgtcg cgcaggcatt aaccgaggct gtggattatg ttcaaccggc gcaacaggac    300

gtgcaacccg caactgcttc cggtatgaaa aatatcgtgg cggttaccgc ttgtccgacc    360

ggggtggcgc acacctttat gtctgccgag gcgattacaa cctactgcca acagcaaggt    420

tggaatgtaa aagtggaaac cagaggtcaa gtcggtgcga acaatattat ttctgcggaa    480

gatgtggcgg cggccgattt agtctttatc gctacggata ttaatgtgga tttaagcaaa    540

ttcaaaggaa aaccgatgta tcgtacttca acgggcttag cattgaagaa aaccgcacag    600

gaatttgata aagcctttaa agaagcgacg atttatcagg gtgaagaaac tacaaccacc    660

acagaaacac aaacttcagg cgagaaaaaa ggtgtatata aacatcttat gaccggggtt    720

tcccatatgt taccgcttgt cgttgccggc ggtttattga ttgctatttc gtttatgttc    780

ggtattgagg cgtttaaaga cgaaaacatc gcaggcggct tgccgaaagc attaatggat    840

atcggcggcg gtgcggcgtt ccacttaatg attgccgtat ttgcaggtta tgttgcattc    900

tctattgcag accgtccggg gttagccgta ggtcttatcg gcggtatgct tgccacatcc    960

gccggtgccg gtattttggg cggtattatc gcgggttttc ttgccggtta tgtagtgaaa   1020

ttcctgaatg atgccattca actgccagcc agtttaactt cgttaaaacc gattttaatt   1080

ctgcctttat taggttcggc gatcgtcggc ttggccatga tttatttatt aaatccaccg   1140

gttgctgcgg caatgaatgc gctaaccgaa tggttaaaag gtttgggctc ggcaaacgcg   1200

ctggtgttgg gtgcgattct tggcggtatg atgtgtatcg atatgggcgg tccggtaaac   1260

aaagccgctt atgtattcgg tacgggcatg attggttcac aggtttatac gccgatggct   1320

gcggtaatgg ctgcgggtat ggtaccgcct ttaggaatgg cgattgccac ctggattgcg   1380

cgcgctaaat ttaacgcaag ccaacgtgat gcgggtaaag cttcattcgt actaggttta   1440

tgctttattt ccgaaggtgc gttaccgttt gttgccgccg accctgtacg cgtgattgtt   1500

tcaagtgtaa ttggcggagc cattgccggc gcaatttcta tgagccttgc cattacgctg   1560

caagcgcctc acggcggttt attcgtgatt ccgtttgtgt cgcaaccgtt aatgtatttg   1620

ggtgcgattg ccgtaggcgc cttaacaacc ggcgttcttt acgcaattat caaaccgaaa   1680

caagctgcgg aataa                                                    1695


<210>  6
<211>  564
<212>  PRT
<213>  Basfia succiniciproducens


<220>
<221>  SITE
<222>  (1)..(564)
<223>  amino acid sequence of FruA from strain DD1

<400>  6

Met Lys Asp Lys Pro Met Asn Ile Phe Leu Thr Gln Ser Pro Asn Leu 
1               5                   10                  15      


Gly Arg Ala Lys Ala Phe Leu Leu His Gln Val Leu Ala Ala Ala Val 
            20                  25                  30          


Lys Gln Gln Asn His Gln Leu Val Glu Asn Ala Glu Gln Ala Asp Leu 
        35                  40                  45              


Ala Ile Val Phe Gly Lys Thr Leu Pro Asn Leu Thr Ala Leu Leu Gly 
    50                  55                  60                  


Lys Lys Val Tyr Leu Ala Asp Glu Glu Gln Ala Leu Asn Ala Pro Glu 
65                  70                  75                  80  


Asn Thr Val Ala Gln Ala Leu Thr Glu Ala Val Asp Tyr Val Gln Pro 
                85                  90                  95      


Ala Gln Gln Asp Val Gln Pro Ala Thr Ala Ser Gly Met Lys Asn Ile 
            100                 105                 110         


Val Ala Val Thr Ala Cys Pro Thr Gly Val Ala His Thr Phe Met Ser 
        115                 120                 125             


Ala Glu Ala Ile Thr Thr Tyr Cys Gln Gln Gln Gly Trp Asn Val Lys 
    130                 135                 140                 


Val Glu Thr Arg Gly Gln Val Gly Ala Asn Asn Ile Ile Ser Ala Glu 
145                 150                 155                 160 


Asp Val Ala Ala Ala Asp Leu Val Phe Ile Ala Thr Asp Ile Asn Val 
                165                 170                 175     


Asp Leu Ser Lys Phe Lys Gly Lys Pro Met Tyr Arg Thr Ser Thr Gly 
            180                 185                 190         


Leu Ala Leu Lys Lys Thr Ala Gln Glu Phe Asp Lys Ala Phe Lys Glu 
        195                 200                 205             


Ala Thr Ile Tyr Gln Gly Glu Glu Thr Thr Thr Thr Thr Glu Thr Gln 
    210                 215                 220                 


Thr Ser Gly Glu Lys Lys Gly Val Tyr Lys His Leu Met Thr Gly Val 
225                 230                 235                 240 


Ser His Met Leu Pro Leu Val Val Ala Gly Gly Leu Leu Ile Ala Ile 
                245                 250                 255     


Ser Phe Met Phe Gly Ile Glu Ala Phe Lys Asp Glu Asn Ile Ala Gly 
            260                 265                 270         


Gly Leu Pro Lys Ala Leu Met Asp Ile Gly Gly Gly Ala Ala Phe His 
        275                 280                 285             


Leu Met Ile Ala Val Phe Ala Gly Tyr Val Ala Phe Ser Ile Ala Asp 
    290                 295                 300                 


Arg Pro Gly Leu Ala Val Gly Leu Ile Gly Gly Met Leu Ala Thr Ser 
305                 310                 315                 320 


Ala Gly Ala Gly Ile Leu Gly Gly Ile Ile Ala Gly Phe Leu Ala Gly 
                325                 330                 335     


Tyr Val Val Lys Phe Leu Asn Asp Ala Ile Gln Leu Pro Ala Ser Leu 
            340                 345                 350         


Thr Ser Leu Lys Pro Ile Leu Ile Leu Pro Leu Leu Gly Ser Ala Ile 
        355                 360                 365             


Val Gly Leu Ala Met Ile Tyr Leu Leu Asn Pro Pro Val Ala Ala Ala 
    370                 375                 380                 


Met Asn Ala Leu Thr Glu Trp Leu Lys Gly Leu Gly Ser Ala Asn Ala 
385                 390                 395                 400 


Leu Val Leu Gly Ala Ile Leu Gly Gly Met Met Cys Ile Asp Met Gly 
                405                 410                 415     


Gly Pro Val Asn Lys Ala Ala Tyr Val Phe Gly Thr Gly Met Ile Gly 
            420                 425                 430         


Ser Gln Val Tyr Thr Pro Met Ala Ala Val Met Ala Ala Gly Met Val 
        435                 440                 445             


Pro Pro Leu Gly Met Ala Ile Ala Thr Trp Ile Ala Arg Ala Lys Phe 
    450                 455                 460                 


Asn Ala Ser Gln Arg Asp Ala Gly Lys Ala Ser Phe Val Leu Gly Leu 
465                 470                 475                 480 


Cys Phe Ile Ser Glu Gly Ala Leu Pro Phe Val Ala Ala Asp Pro Val 
                485                 490                 495     


Arg Val Ile Val Ser Ser Val Ile Gly Gly Ala Ile Ala Gly Ala Ile 
            500                 505                 510         


Ser Met Ser Leu Ala Ile Thr Leu Gln Ala Pro His Gly Gly Leu Phe 
        515                 520                 525             


Val Ile Pro Phe Val Ser Gln Pro Leu Met Tyr Leu Gly Ala Ile Ala 
    530                 535                 540                 


Val Gly Ala Leu Thr Thr Gly Val Leu Tyr Ala Ile Ile Lys Pro Lys 
545                 550                 555                 560 


Gln Ala Ala Glu 
                


<210>  7
<211>  315
<212>  DNA
<213>  Basfia succiniciproducens


<220>
<221>  MISC_FEATURE
<223>  nucleotide sequence of ackA-promoter from strain DD1

<400>  7
ccgaatattt ctgccccgcc gatatagttt taaaaaatcc catttttaaa tccttaattt     60

atatttcttc aaatttttac tactgactta ttttaaattt gctgccattt tatcctgttt    120

taaagatcgc ttatcgcatg aaactcaaat ggcttattgg atatatgtcc taaaaattga    180

acatttttat aacattttgc taaaaattca attttttttg acttaaatcg cttttttcag    240

aacttcataa tttattttat tccgttaaac gtgtatcctt attatcggta acattcccaa    300

acgtctattc gttca                                                     315


<210>  8
<211>  1029
<212>  DNA
<213>  Basfia succinicproducens


<220>
<221>  MISC_FEATURE
<223>  nucleotide sequence of ldhA-gene from strain DD1

<400>  8
ttgacaaaat cagtatgttt aaataaggag ctaactatga aagttgccgt ttacagtact     60

aaaaattatg atcgcaaaca tctggatttg gcgaataaaa aatttaattt tgagcttcat    120

ttctttgatt ttttacttga tgaacaaacc gcgaaaatgg cggagggcgc cgatgccgtc    180

tgtattttcg tcaatgatga tgcgagccgc ccggtgttaa caaagttggc gcaaatcgga    240

gtgaaaatta tcgctttacg ttgtgccggt tttaataatg tggatttgga ggcggcaaaa    300

gagctgggat taaaagtcgt acgggtgcct gcgtattcgc cggaagccgt tgccgagcat    360

gcgatcggat taatgctgac tttaaaccgc cgtatccata aggcttatca gcgtacccgc    420

gatgcgaatt tttctctgga aggattggtc ggttttaata tgttcggcaa aaccgccgga    480

gtgattggta cgggaaaaat cggcttggcg gctattcgca ttttaaaagg cttcggtatg    540

gacgttctgg cgtttgatcc ttttaaaaat ccggcggcgg aagcgttggg cgcaaaatat    600

gtcggtttag acgagcttta tgcaaaatcc catgttatca ctttgcattg cccggctacg    660

gcggataatt atcatttatt aaatgaagcg gcttttaata aaatgcgcga cggtgtaatg    720

attattaata ccagccgcgg cgttttaatt gacagccggg cggcaatcga agcgttaaaa    780

cggcagaaaa tcggcgctct cggtatggat gtttatgaaa atgaacggga tttgtttttc    840

gaggataaat ctaacgatgt tattacggat gatgtattcc gtcgcctttc ttcctgtcat    900

aatgtgcttt ttaccggtca tcaggcgttt ttaacggaag aagcgctgaa taatatcgcc    960

gatgtgactt tatcgaatat tcaggcggtt tccaaaaatg caacgtgcga aaatagcgtt   1020

gaaggctaa                                                           1029


<210>  9
<211>  342
<212>  PRT
<213>  Basfia succiniciproducens


<220>
<221>  SITE
<222>  (1)..(342)
<223>  amino acid sequence of LdhA from strain DD1

<400>  9

Met Thr Lys Ser Val Cys Leu Asn Lys Glu Leu Thr Met Lys Val Ala 
1               5                   10                  15      


Val Tyr Ser Thr Lys Asn Tyr Asp Arg Lys His Leu Asp Leu Ala Asn 
            20                  25                  30          


Lys Lys Phe Asn Phe Glu Leu His Phe Phe Asp Phe Leu Leu Asp Glu 
        35                  40                  45              


Gln Thr Ala Lys Met Ala Glu Gly Ala Asp Ala Val Cys Ile Phe Val 
    50                  55                  60                  


Asn Asp Asp Ala Ser Arg Pro Val Leu Thr Lys Leu Ala Gln Ile Gly 
65                  70                  75                  80  


Val Lys Ile Ile Ala Leu Arg Cys Ala Gly Phe Asn Asn Val Asp Leu 
                85                  90                  95      


Glu Ala Ala Lys Glu Leu Gly Leu Lys Val Val Arg Val Pro Ala Tyr 
            100                 105                 110         


Ser Pro Glu Ala Val Ala Glu His Ala Ile Gly Leu Met Leu Thr Leu 
        115                 120                 125             


Asn Arg Arg Ile His Lys Ala Tyr Gln Arg Thr Arg Asp Ala Asn Phe 
    130                 135                 140                 


Ser Leu Glu Gly Leu Val Gly Phe Asn Met Phe Gly Lys Thr Ala Gly 
145                 150                 155                 160 


Val Ile Gly Thr Gly Lys Ile Gly Leu Ala Ala Ile Arg Ile Leu Lys 
                165                 170                 175     


Gly Phe Gly Met Asp Val Leu Ala Phe Asp Pro Phe Lys Asn Pro Ala 
            180                 185                 190         


Ala Glu Ala Leu Gly Ala Lys Tyr Val Gly Leu Asp Glu Leu Tyr Ala 
        195                 200                 205             


Lys Ser His Val Ile Thr Leu His Cys Pro Ala Thr Ala Asp Asn Tyr 
    210                 215                 220                 


His Leu Leu Asn Glu Ala Ala Phe Asn Lys Met Arg Asp Gly Val Met 
225                 230                 235                 240 


Ile Ile Asn Thr Ser Arg Gly Val Leu Ile Asp Ser Arg Ala Ala Ile 
                245                 250                 255     


Glu Ala Leu Lys Arg Gln Lys Ile Gly Ala Leu Gly Met Asp Val Tyr 
            260                 265                 270         


Glu Asn Glu Arg Asp Leu Phe Phe Glu Asp Lys Ser Asn Asp Val Ile 
        275                 280                 285             


Thr Asp Asp Val Phe Arg Arg Leu Ser Ser Cys His Asn Val Leu Phe 
    290                 295                 300                 


Thr Gly His Gln Ala Phe Leu Thr Glu Glu Ala Leu Asn Asn Ile Ala 
305                 310                 315                 320 


Asp Val Thr Leu Ser Asn Ile Gln Ala Val Ser Lys Asn Ala Thr Cys 
                325                 330                 335     


Glu Asn Ser Val Glu Gly 
            340         


<210>  10
<211>  741
<212>  DNA
<213>  Basfia succiniciproducens


<220>
<221>  MISC_FEATURE
<223>  nucleotide sequence of pflA-gene from strain DD1

<400>  10
atgtcggttt taggacgaat tcattcattt gaaacctgcg ggacagttga cgggccggga     60

atccgcttta ttttattttt acaaggctgc ttaatgcgtt gtaaatactg ccataataga    120

gacacctggg atttgcacgg cggtaaagaa atttccgttg aagaattaat gaaagaagtg    180

gtgacctatc gccattttat gaacgcctcg ggcggcggag ttaccgcttc cggcggtgaa    240

gctattttac aggcggaatt tgtacgggac tggttcagag cctgccataa agaaggaatt    300

aatacttgct tggataccaa cggtttcgtc cgtcatcatg atcatattat tgatgaattg    360

attgatgaca cggatcttgt gttgcttgac ctgaaagaaa tgaatgaacg ggttcacgaa    420

agcctgattg gcgtgccgaa taaaagagtg ctcgaattcg caaaatattt agcggatcga    480

aatcagcgta cctggatccg ccatgttgta gtgccgggtt atacagatag tgacgaagat    540

ttgcacatgc tggggaattt cattaaagat atgaagaata tcgaaaaagt ggaattatta    600

ccttatcacc gtctaggcgc ccataaatgg gaagtactcg gcgataaata cgagcttgaa    660

gatgtaaaac cgccgacaaa agaattaatg gagcatgtta aggggttgct tgcaggctac    720

gggcttaatg tgacatatta g                                              741


<210>  11
<211>  246
<212>  PRT
<213>  Basfia succiniciproducens


<220>
<221>  SITE
<222>  (1)..(246)
<223>  amino acid of PflA from strain DD1

<400>  11

Met Ser Val Leu Gly Arg Ile His Ser Phe Glu Thr Cys Gly Thr Val 
1               5                   10                  15      


Asp Gly Pro Gly Ile Arg Phe Ile Leu Phe Leu Gln Gly Cys Leu Met 
            20                  25                  30          


Arg Cys Lys Tyr Cys His Asn Arg Asp Thr Trp Asp Leu His Gly Gly 
        35                  40                  45              


Lys Glu Ile Ser Val Glu Glu Leu Met Lys Glu Val Val Thr Tyr Arg 
    50                  55                  60                  


His Phe Met Asn Ala Ser Gly Gly Gly Val Thr Ala Ser Gly Gly Glu 
65                  70                  75                  80  


Ala Ile Leu Gln Ala Glu Phe Val Arg Asp Trp Phe Arg Ala Cys His 
                85                  90                  95      


Lys Glu Gly Ile Asn Thr Cys Leu Asp Thr Asn Gly Phe Val Arg His 
            100                 105                 110         


His Asp His Ile Ile Asp Glu Leu Ile Asp Asp Thr Asp Leu Val Leu 
        115                 120                 125             


Leu Asp Leu Lys Glu Met Asn Glu Arg Val His Glu Ser Leu Ile Gly 
    130                 135                 140                 


Val Pro Asn Lys Arg Val Leu Glu Phe Ala Lys Tyr Leu Ala Asp Arg 
145                 150                 155                 160 


Asn Gln Arg Thr Trp Ile Arg His Val Val Val Pro Gly Tyr Thr Asp 
                165                 170                 175     


Ser Asp Glu Asp Leu His Met Leu Gly Asn Phe Ile Lys Asp Met Lys 
            180                 185                 190         


Asn Ile Glu Lys Val Glu Leu Leu Pro Tyr His Arg Leu Gly Ala His 
        195                 200                 205             


Lys Trp Glu Val Leu Gly Asp Lys Tyr Glu Leu Glu Asp Val Lys Pro 
    210                 215                 220                 


Pro Thr Lys Glu Leu Met Glu His Val Lys Gly Leu Leu Ala Gly Tyr 
225                 230                 235                 240 


Gly Leu Asn Val Thr Tyr 
                245     


<210>  12
<211>  2313
<212>  DNA
<213>  Basfia succiniciproducens


<220>
<221>  misc_feature
<223>  nucleotide sequence of pflD-gene from strain DD1

<400>  12
atggctgaat taacagaagc tcaaaaaaaa gcatgggaag gattcgttcc cggtgaatgg     60

caaaacggcg taaatttacg tgactttatc caaaaaaact atactccgta tgaaggtgac    120

gaatcattct tagctgatgc gactcctgca accagcgagt tgtggaacag cgtgatggaa    180

ggcatcaaaa tcgaaaacaa aactcacgca cctttagatt tcgacgaaca tactccgtca    240

actatcactt ctcacaagcc tggttatatc aataaagatt tagaaaaaat cgttggtctt    300

caaacagacg ctccgttaaa acgtgcaatt atgccgtacg gcggtatcaa aatgatcaaa    360

ggttcttgcg aagtttacgg tcgtaaatta gatccgcaag tagaatttat tttcaccgaa    420

tatcgtaaaa cccataacca aggcgtattc gacgtttata cgccggatat tttacgctgc    480

cgtaaatcag gcgtgttaac cggtttaccg gatgcttacg gtcgtggtcg tattatcggt    540

gactaccgtc gtttagcggt atacggtatt gattacctga tgaaagataa aaaagcccaa    600

ttcgattcat tacaaccgcg tttggaagcg ggcgaagaca ttcaggcaac tatccaatta    660

cgtgaagaaa ttgccgaaca acaccgcgct ttaggcaaaa tcaaagaaat ggcggcatct    720

tacggttacg acatttccgg ccctgcgaca aacgcacagg aagcaatcca atggacatat    780

tttgcttatc tggcagcggt taaatcacaa aacggtgcgg caatgtcatt cggtcgtacg    840

tctacattct tagatatcta tatcgaacgt gacttaaaac gcggtttaat cactgaacaa    900

caggcgcagg aattaatgga ccacttagta atgaaattac gtatggttcg tttcttacgt    960

acgccggaat acgatcaatt attctcaggc gacccgatgt gggcaaccga aactatcgcc   1020

ggtatgggct tagacggtcg tccgttggta actaaaaaca gcttccgcgt attacatact   1080

ttatacacta tgggtacttc tccggaacca aacttaacta ttctttggtc cgaacaatta   1140

cctgaagcgt tcaaacgttt ctgtgcgaaa gtatctattg atacttcctc cgtacaatac   1200

gaaaatgatg acttaatgcg tcctgacttc aacaacgatg actatgcaat cgcatgctgc   1260

gtatcaccga tggtcgtagg taaacaaatg caattcttcg gtgcgcgcgc aaacttagct   1320

aaaactatgt tatacgcaat taacggcggt atcgatgaga aaaatggtat gcaagtcggt   1380

cctaaaactg cgccgattac agacgaagta ttgaatttcg ataccgtaat cgaacgtatg   1440

gacagtttca tggactggtt ggcgactcaa tatgtaaccg cattgaacat catccacttc   1500

atgcacgata aatatgcata tgaagcggca ttgatggcgt tccacgatcg cgacgtattc   1560

cgtacaatgg cttgcggtat cgcgggtctt tccgtggctg cggactcatt atccgcaatc   1620

aaatatgcga aagttaaacc gattcgcggc gacatcaaag ataaagacgg taatgtcgtg   1680

gcctcgaatg ttgctatcga cttcgaaatt gaaggcgaat atccgcaatt cggtaacaat   1740

gatccgcgtg ttgatgattt agcggtagac ttagttgaac gtttcatgaa aaaagttcaa   1800

aaacacaaaa cttaccgcaa cgcaactccg acacaatcta tcctgactat cacttctaac   1860

gtggtatacg gtaagaaaac cggtaatact ccggacggtc gtcgagcagg cgcgccattc   1920

ggaccgggtg caaacccaat gcacggtcgt gaccaaaaag gtgcggttgc ttcacttact   1980

tctgtggcta aacttccgtt cgcttacgcg aaagacggta tttcatatac cttctctatc   2040

gtaccgaacg cattaggtaa agatgacgaa gcgcaaaaac gcaaccttgc cggtttaatg   2100

gacggttatt tccatcatga agcgacagtg gaaggcggtc aacacttgaa tgttaacgtt   2160

cttaaccgtg aaatgttgtt agacgcgatg gaaaatccgg aaaaataccc gcaattaacc   2220

attcgtgttt caggttacgc ggttcgtttc aactcattaa ctaaagagca acaacaagac   2280

gtcatcactc gtacgtttac acaatcaatg taa                                2313


<210>  13
<211>  770
<212>  PRT
<213>  Basfia succinicproducens


<220>
<221>  SITE
<222>  (1)..(770)
<223>  amino acid of PflD from strain DD1

<400>  13

Met Ala Glu Leu Thr Glu Ala Gln Lys Lys Ala Trp Glu Gly Phe Val 
1               5                   10                  15      


Pro Gly Glu Trp Gln Asn Gly Val Asn Leu Arg Asp Phe Ile Gln Lys 
            20                  25                  30          


Asn Tyr Thr Pro Tyr Glu Gly Asp Glu Ser Phe Leu Ala Asp Ala Thr 
        35                  40                  45              


Pro Ala Thr Ser Glu Leu Trp Asn Ser Val Met Glu Gly Ile Lys Ile 
    50                  55                  60                  


Glu Asn Lys Thr His Ala Pro Leu Asp Phe Asp Glu His Thr Pro Ser 
65                  70                  75                  80  


Thr Ile Thr Ser His Lys Pro Gly Tyr Ile Asn Lys Asp Leu Glu Lys 
                85                  90                  95      


Ile Val Gly Leu Gln Thr Asp Ala Pro Leu Lys Arg Ala Ile Met Pro 
            100                 105                 110         


Tyr Gly Gly Ile Lys Met Ile Lys Gly Ser Cys Glu Val Tyr Gly Arg 
        115                 120                 125             


Lys Leu Asp Pro Gln Val Glu Phe Ile Phe Thr Glu Tyr Arg Lys Thr 
    130                 135                 140                 


His Asn Gln Gly Val Phe Asp Val Tyr Thr Pro Asp Ile Leu Arg Cys 
145                 150                 155                 160 


Arg Lys Ser Gly Val Leu Thr Gly Leu Pro Asp Ala Tyr Gly Arg Gly 
                165                 170                 175     


Arg Ile Ile Gly Asp Tyr Arg Arg Leu Ala Val Tyr Gly Ile Asp Tyr 
            180                 185                 190         


Leu Met Lys Asp Lys Lys Ala Gln Phe Asp Ser Leu Gln Pro Arg Leu 
        195                 200                 205             


Glu Ala Gly Glu Asp Ile Gln Ala Thr Ile Gln Leu Arg Glu Glu Ile 
    210                 215                 220                 


Ala Glu Gln His Arg Ala Leu Gly Lys Ile Lys Glu Met Ala Ala Ser 
225                 230                 235                 240 


Tyr Gly Tyr Asp Ile Ser Gly Pro Ala Thr Asn Ala Gln Glu Ala Ile 
                245                 250                 255     


Gln Trp Thr Tyr Phe Ala Tyr Leu Ala Ala Val Lys Ser Gln Asn Gly 
            260                 265                 270         


Ala Ala Met Ser Phe Gly Arg Thr Ser Thr Phe Leu Asp Ile Tyr Ile 
        275                 280                 285             


Glu Arg Asp Leu Lys Arg Gly Leu Ile Thr Glu Gln Gln Ala Gln Glu 
    290                 295                 300                 


Leu Met Asp His Leu Val Met Lys Leu Arg Met Val Arg Phe Leu Arg 
305                 310                 315                 320 


Thr Pro Glu Tyr Asp Gln Leu Phe Ser Gly Asp Pro Met Trp Ala Thr 
                325                 330                 335     


Glu Thr Ile Ala Gly Met Gly Leu Asp Gly Arg Pro Leu Val Thr Lys 
            340                 345                 350         


Asn Ser Phe Arg Val Leu His Thr Leu Tyr Thr Met Gly Thr Ser Pro 
        355                 360                 365             


Glu Pro Asn Leu Thr Ile Leu Trp Ser Glu Gln Leu Pro Glu Ala Phe 
    370                 375                 380                 


Lys Arg Phe Cys Ala Lys Val Ser Ile Asp Thr Ser Ser Val Gln Tyr 
385                 390                 395                 400 


Glu Asn Asp Asp Leu Met Arg Pro Asp Phe Asn Asn Asp Asp Tyr Ala 
                405                 410                 415     


Ile Ala Cys Cys Val Ser Pro Met Val Val Gly Lys Gln Met Gln Phe 
            420                 425                 430         


Phe Gly Ala Arg Ala Asn Leu Ala Lys Thr Met Leu Tyr Ala Ile Asn 
        435                 440                 445             


Gly Gly Ile Asp Glu Lys Asn Gly Met Gln Val Gly Pro Lys Thr Ala 
    450                 455                 460                 


Pro Ile Thr Asp Glu Val Leu Asn Phe Asp Thr Val Ile Glu Arg Met 
465                 470                 475                 480 


Asp Ser Phe Met Asp Trp Leu Ala Thr Gln Tyr Val Thr Ala Leu Asn 
                485                 490                 495     


Ile Ile His Phe Met His Asp Lys Tyr Ala Tyr Glu Ala Ala Leu Met 
            500                 505                 510         


Ala Phe His Asp Arg Asp Val Phe Arg Thr Met Ala Cys Gly Ile Ala 
        515                 520                 525             


Gly Leu Ser Val Ala Ala Asp Ser Leu Ser Ala Ile Lys Tyr Ala Lys 
    530                 535                 540                 


Val Lys Pro Ile Arg Gly Asp Ile Lys Asp Lys Asp Gly Asn Val Val 
545                 550                 555                 560 


Ala Ser Asn Val Ala Ile Asp Phe Glu Ile Glu Gly Glu Tyr Pro Gln 
                565                 570                 575     


Phe Gly Asn Asn Asp Pro Arg Val Asp Asp Leu Ala Val Asp Leu Val 
            580                 585                 590         


Glu Arg Phe Met Lys Lys Val Gln Lys His Lys Thr Tyr Arg Asn Ala 
        595                 600                 605             


Thr Pro Thr Gln Ser Ile Leu Thr Ile Thr Ser Asn Val Val Tyr Gly 
    610                 615                 620                 


Lys Lys Thr Gly Asn Thr Pro Asp Gly Arg Arg Ala Gly Ala Pro Phe 
625                 630                 635                 640 


Gly Pro Gly Ala Asn Pro Met His Gly Arg Asp Gln Lys Gly Ala Val 
                645                 650                 655     


Ala Ser Leu Thr Ser Val Ala Lys Leu Pro Phe Ala Tyr Ala Lys Asp 
            660                 665                 670         


Gly Ile Ser Tyr Thr Phe Ser Ile Val Pro Asn Ala Leu Gly Lys Asp 
        675                 680                 685             


Asp Glu Ala Gln Lys Arg Asn Leu Ala Gly Leu Met Asp Gly Tyr Phe 
    690                 695                 700                 


His His Glu Ala Thr Val Glu Gly Gly Gln His Leu Asn Val Asn Val 
705                 710                 715                 720 


Leu Asn Arg Glu Met Leu Leu Asp Ala Met Glu Asn Pro Glu Lys Tyr 
                725                 730                 735     


Pro Gln Leu Thr Ile Arg Val Ser Gly Tyr Ala Val Arg Phe Asn Ser 
            740                 745                 750         


Leu Thr Lys Glu Gln Gln Gln Asp Val Ile Thr Arg Thr Phe Thr Gln 
        755                 760                 765             


Ser Met 
    770 


<210>  14
<211>  30
<212>  DNA
<213>  artificial sequence

<220>
<223>  nucleotide sequence of primer PRfruA1

<400>  14
tgctctagat gcggaagaga gcctttccgg                                      30


<210>  15
<211>  64
<212>  DNA
<213>  artificial sequence

<220>
<223>  nucleotide sequence of primer PRfruA2

<400>  15
caccaggttt tggctgccgc agtaaaacaa tttcctaatc aagcataaag cctttgttta     60

tctc                                                                  64


<210>  16
<211>  64
<212>  DNA
<213>  artificial sequence

<220>
<223>  nucleotide sequence of primer PRfruA3

<400>  16
gagataaaca aaggctttat gcttgattag gaaattgttt tactgcggca gccaaaacct     60

ggtg                                                                  64


<210>  17
<211>  35
<212>  DNA
<213>  artificial sequence

<220>
<223>  nucleotide sequence of primer PRfruA4

<400>  17
ccgctcgagt aggagtaact caaggtcacc gtttg                                35


<210>  18
<211>  34
<212>  DNA
<213>  artificial sequence

<220>
<223>  nucleotide sequence of primer PRrbsK1

<400>  18
ggcggccgct ctagaccgaa tatttctgcc ccgc                                 34


<210>  19
<211>  42
<212>  DNA
<213>  artificial sequence

<220>
<223>  nucleotide sequence of primer PRrbsK2

<400>  19
gtttgtcata tgcattgaac gaatagacgt ttgggaatgt ta                        42


<210>  20
<211>  46
<212>  DNA
<213>  artificial sequence

<220>
<223>  nucleotide sequence of primer PRrbsK3

<400>  20
acgtctattc gttcaatgca tatgacaaac aaaatttggg tattag                    46


<210>  21
<211>  39
<212>  DNA
<213>  artificial sequence

<220>
<223>  nucleotide sequence of primer PRrbsK4

<400>  21
gggccccccc tcgagcctag cttaaagata gccggtaaa                            39


<210>  22
<211>  9517
<212>  DNA
<213>  artificial sequence

<220>
<223>  nucleotide sequence of plasmid pJFF224_PackArbsK

<400>  22
ccccggagtg gttcgacggc ctcaagcgcg ccgccgaggg ccgccgcctg atggtgctgg     60

acacgctgcg ccggttccac atcgaggaag aaaacgccag cggccccatg gcccaggtca    120

tcggtcgcat ggaggccatc gccgccgata ccgggtgctc tatcgtgttc ctgcaccatg    180

ccagcaaggg cgcggccatg atgggcgcag gcgaccagca gcaggccagc cggggcagct    240

cggtactggt cgataacatc cgctggcagt cctacctgtc gagcatgacc agcgccgagg    300

ccgaggaatg gggtgtggac gacgaccagc gccggttctt cgtccgcttc ggtgtgagca    360

aggccaacta tggcgcaccg ttcgctgatc ggtggttcag gcggcatgac ggcggggtgc    420

tcaagcccgc cgtgctggag aggcagcgca agagcaaggg ggtgccccgt ggtgaagcct    480

aagaacaagc acagcctcag ccacgtccgg cacgacccgg cgcactgtct ggcccccggc    540

ctgttccgtg ccctcaagcg gggcgagcgc aagcgcagca agctggacgt gacgtatgac    600

tacggcgacg gcaagcggat cgagttcagc ggcccggagc cgctgggcgc tgatgatctg    660

cgcatcctgc aagggctggt ggccatggct gggcctaatg gcctagtgct tggcccggaa    720

cccaagaccg aaggcggacg gcagctccgg ctgttcctgg aacccaagtg ggaggccgtc    780

accgctgatg ccatggtggt caaaggtagc tatcgggcgc tggcaaagga aatcggggca    840

gaggtcgata gtggtggggc gctcaagcac atacaggact gcatcgagcg cctttggaag    900

gtatccatca tcgcccagaa tggccgcaag cggcaggggt ttcggctgct gtcggagtac    960

gccagcgacg aggcggacgg gcgcctgtac gtggccctga accccttgat cgcgcaggcc   1020

gtcatgggtg gcggccagca tgtgcgcatc agcatggacg aggtgcgggc gctggacagc   1080

gaaaccgccc gcctgctgca ccagcggctg tgtggctgga tcgaccccgg caaaaccggc   1140

aaggcttcca tagatacctt gtgcggctat gtctggccgt cagaggccag tggttcgacc   1200

atgcgcaagc gccgccagcg ggtgcgcgag gcgttgccgg agctggtcgc gctgggctgg   1260

acggtaaccg agttcgcggc gggcaagtac gacatcaccc ggcccaaggc ggcaggctga   1320

ccccccccac tctattgtaa acaagacatt ttttatcttt tatattcaat ggcttatttt   1380

cctgctaatt ggtaatacca tgaaaaatac catgctcaga aaaggcttaa caatattttg   1440

aaaaattgcc tactgagcgc tgccgcacag ctccataggc cgctttcctg gctttgcttc   1500

cagatgtatg ctctcctccg gagagtaccg tgactttatt ttcggcacaa atacaggggt   1560

cgatggataa atacggcgat agtttcctga cggatgatcc gtatgtaccg gcggaagaca   1620

agctgcaaac ctgtcagatg gagattgatt taatggcgga tgtgctgaga gcaccgcccc   1680

gtgaatccgc agaactgatc cgctatgtgt ttgcggatga ttggccggaa taaataaagc   1740

cgggcttaat acagattaag cccgtatagg gtattattac tgaataccaa acagcttacg   1800

gaggacggaa tgttacccat tgagacaacc agactgcctt ctgattatta atatttttca   1860

ctattaatca gaaggaataa ccatgaattt tacccggatt gacctgaata cctggaatcg   1920

cagggaacac tttgcccttt atcgtcagca gattaaatgc ggattcagcc tgaccaccaa   1980

actcgatatt accgctttgc gtaccgcact ggcggagaca ggttataagt tttatccgct   2040

gatgatttac ctgatctccc gggctgttaa tcagtttccg gagttccgga tggcactgaa   2100

agacaatgaa cttatttact gggaccagtc agacccggtc tttactgtct ttcataaaga   2160

aaccgaaaca ttctctgcac tgtcctgccg ttattttccg gatctcagtg agtttatggc   2220

aggttataat gcggtaacgg cagaatatca gcatgatacc agattgtttc cgcagggaaa   2280

tttaccggag aatcacctga atatatcatc attaccgtgg gtgagttttg acgggattta   2340

acctgaacat caccggaaat gatgattatt ttgccccggt ttttacgatg gcaaagtttc   2400

agcaggaagg tgaccgcgta ttattacctg tttctgtaca ggttcatcat gcagtctgtg   2460

atggctttca tgcagcacgg tttattaata cacttcagct gatgtgtgat aacatactga   2520

aataaattaa ttaattctgt atttaagcca ccgtatccgg caggaatggt ggcttttttt   2580

ttatatttta accgtaatct gtaatttcgt ttcagactgg ttcaggatca ctgtacgata   2640

atgcccccgc agtttggtaa tacccttaat aaaaaagaaa cagcaaagac tgacagcaat   2700

aataataaag taagcagtaa caataatatt aacaacacca gatgcagtta taataatagt   2760

atttaagaca ccagaaagac tgctgcgaca gtcattttga acaacaccaa aatgccgtaa   2820

aggcagtagt aacaacacca gtgaaaacat cacgatagca tagtgatatg cctgagtgtg   2880

tgtaattaaa caataaataa accgccatat ataacagaag atagtattct gaatggcatg   2940

cttttctgtt cagtataaac atatcatccc ggttggtata aggatgatat ataataagtt   3000

aagctgaaca catatttatt ttggttttat tttacaaata aagtaagacg atccgttaag   3060

tcaaagcggg gtatatttat tataccctgc ttttttattt gtccgccggg cgcggataat   3120

ggatcagatt atgcagtgtc acaatggcct taccgggatt ggcgtaagcg tgcgggatat   3180

ccgcatggaa gcgcagggat tccccggcag aaacggtgtg ccactcatcc cccagccgca   3240

gttgtaatgc gccttccagt acaatgacat gttctctggt tctgaaatcc atccctgtcg   3300

gtgttgctta tgcagtctgg tcgggactcg gcgtcgtcat aattacagcc attgcctggt   3360

tgcttcatgg gcaaaagctt tatgcttgta aaccgttttg tgaaaaaatt tttaaaataa   3420

aaaaggggac ctctagggtc cccaattaat tagtaatata atctattaaa ggtcattcaa   3480

aaggtcatcc accggatccc accgcggtgg cggccgctct agaccgaata tttctgcccc   3540

gccgatatag ttttaaaaaa tcccattttt aaatccttaa tttatatttc ttcaaatttt   3600

tactactgac ttattttaaa tttgctgcca ttttatcctg ttttaaagat cgcttatcgc   3660

atgaaactca aatggcttat tggatatatg tcctaaaaat tgaacatttt tataacattt   3720

tgctaaaaat tcaatttttt ttgacttaaa tcgctttttt cagaacttca taatttattt   3780

tattccgtta aacgtgtatc cttattatcg gtaacattcc caaacgtcta ttcgttcaat   3840

gcatatgaca aacaaaattt gggtattagg cgatgccgtg gtggatttaa ttcctgacgg   3900

agacaaccat tatttgcgtt gcgcaggcgg cgcaccggct aatgtggcgg tcggcgttgc   3960

ccgtttaggt gtgcctagcg catttatcgg ccgtgtaggt aaagatccgt taggggaatt   4020

tatgcgcgat acgctgaatc aggaaaatgt aaacaccgat tatatgttgt tagatcctaa   4080

acaacgtact tcgacggtgg tggttggatt aaccgacggc gaacgtagtt ttacctttat   4140

ggtgaatcca agtgcggatc aatttttaca aatttccgat ctgccgcaat ttcaagccgg   4200

agactggttg cactgctgct ctatcgcctt aatcaatgaa ccgacccgca gcgctacttt   4260

cacggcaatg aaaaatatcc gtgcggccgg cggtaaagta tctttcgatc cgaatttacg   4320

cgaaagctta tggaaatccc aggatgaaat gatcgatgtg gtgatggaag cggtaagcct   4380

tgccgacgta ttgaaatttt cagaagaaga attaacgctg ttaacccata ccgacagcct   4440

ggaaaaatct tttgaaaaaa tcaccgcact ttatcccgat aaattgatta ttgtcacttt   4500

agggaaagat ggcgcgctct atcatctgca cggtaaaaaa gaggtggttg cagggaaagc   4560

gctgaaaccg gtagatacca ccggtgccgg cgacgctttt gtcagcgggt tattagccgg   4620

attatcacaa acggaaaact ggcagcaacc tgaacaactc gttactatta ttcgccaggc   4680

caacgccagc ggcgcgcttg ccacaacggc aaaaggcgct atgtcggcat taccgaatcg   4740

gcaacaatta gcggaatttt tagcaaacta aaattcgcaa aatttattcc aaactcattc   4800

tccgattatt aattgcatca acttaaaaag taaaaatgca gtgcgttaag cggcctatgt   4860

gagtgttccg ctaaatctgc acgaattaaa gaacgtttat atgcccctaa tcggcaggtt   4920

atatccattg attagtacag tacaaaaaag tccggcgctt atcaccggac ttttttatgc   4980

ctaaaaatag aatttggtat aaatatattg ttttttatat ttaccggcta tctttaagct   5040

aggctcgagg gggggcccgg atccccagta gatttacgtt taaacatttt tatttccttt   5100

ttaatttaat ttaattaaca gttggtgcta tgacacttta cctcatagct ggcataattc   5160

gcaatactct gggtcttcga gaggtatcca acctgagttg aaatacttta ccatcgattt   5220

agcagttgta tcagttatat ttatattacc tttaactctt cgccatccag gagttttacc   5280

gtacagatta gaggataata ataacacata attctcgtaa gcaatatgag ataatttcca   5340

agactctata ttagctcgtg atgttttcca aggtctaaaa tcgtcacggt tcatataatt   5400

agccaatctc atatgctctc taacttccga tgataagctg tcaaacatga gaattaacga   5460

tctgatagag aagggtttgc tcgggtcggt ggctctggta acgaccagta tcccgatccc   5520

ggctggccgt cctggccgcc acatgaggca tgttccgcgt ccttgcaata ctgtgtttac   5580

atacagtcta tcgcttagcg gaaagttctt ttaccctcag ccgaaatgcc tgccgttgct   5640

agacattgcc agccagtgcc cgtcactccc gtactaactg tcacgaaccc ctgcaataac   5700

tgtcacgccc ccctgcaata actgtcacga acccctgcaa taactgtcac gcccccaaac   5760

ctgcaaaccc agcaggggcg ggggctggcg gggtgttgga aaaatccatc catgattatc   5820

taagaataat ccactaggcg cggttatcag cgcccttgtg gggcgctgct gcccttgccc   5880

aatatgcccg gccagaggcc ggatagctgg tctattcgct gcgctaggct acacaccgcc   5940

ccaccgctgc gcggcagggg gaaaggcggg caaagcccgc taaaccccac accaaacccc   6000

gcagaaatac gctgggagcg cttttagccg ctttagcggc ctttccccct acccgaaggg   6060

tgggggcgcg tgtgcagccc cgcagggcct gtctcggtcg atcattcagc ccggctcatc   6120

cttctggcgt ggcggcagac cgaacaaggc gcggtcgtgg tcgcgttcaa ggtacgcatc   6180

cattgccgcc atgagccgat cctccggcca ctcgctgctg ttcaccttgg ccaaaatcat   6240

ggcccccacc agcaccttgc gccttgtttc gttcttgcgc tattgctgct gttcccttgc   6300

ccgcacccgc tgaatttcgg cattgattcg cgctcgttgt tcttcgagct tggccagccg   6360

atccgccgcc ttgttgctcc ccttaaccat cttgacaccc cattgttaat gtgctgtctc   6420

gtaggctatc atggaggcac agcggcggca atcccgaccc tactttgtag gggagggcca   6480

ttgcatggag ccgaaaagca aaagcaacag cgaggcagca tggcgattta tcaccttacg   6540

gcgaaaaccg gcagcaggtc gggcggccaa tcggccaggg ccaaggccga ctacatccag   6600

cgcgaaggca agtatgcccg cgacatggat gaagtcttgc acgccgaatc cgggcacatg   6660

ccggagttcg tcgagcggcc cgccgactac tgggatgctg ccgacctgta tgaacgcgcc   6720

aatgggcggc tgttcaagga ggtcgaattt gccctgccgg tcgagctgac cctcgaccag   6780

cagaaggcgc tggcgtccga gttcgcccag cacctgaccg gtgccgagcg cctgccgtat   6840

acgctggcca tccatgccgg tggcggcgag aacccgcact gccacctgat gatctccgag   6900

cggatcaatg acggcatcga gcggcccgcc gctcagtggt tcaagcggta caacggcaag   6960

accccggaga agggcggggc acagaagacc gaagcgctca agcccaaggc atggcttgag   7020

cagacccgcg aggcatgggc cgaccatgcc aaccgggcat tagagcgggc tggccacgac   7080

gcccgcattg accacagaac acttgaggcg cagggcatcg agcgcctgcc cggtgttcac   7140

ctggggccga acgtggtgga gatggaaggc cggggcatcc gcaccgaccg ggcagacgtg   7200

gccctgaaca tcgacaccgc caacgcccag atcatcgact tacaggaata ccgggaggca   7260

atagaccatg aacgcaatcg acagagtgaa gaaatccaga ggcatcaacg agttagcgga   7320

gcagatcgaa ccgctggccc agagcatggc gacactggcc gacgaagccc ggcaggtcat   7380

gagccagacc cagcaggcca gcgaggcgca ggcggcggag tggctgaaag cccagcgcca   7440

gacaggggcg gcatgggtgg agctggccaa agagttgcgg gaggtagccg ccgaggtgag   7500

cagcgccgcg cagagcgccc ggagcgcgtc gcgggggtgg cactggaagc tatggctaac   7560

cgtgatgctg gcttccatga tgcctacggt ggtgctgctg atcgcatcgt tgctcttgct   7620

cgacctgacg ccactgacaa ccgaggacgg ctcgatctgg ctgcgcttgg tggcccgatg   7680

aagaacgaca ggactttgca ggccataggc cgacagctca aggccatggg ctgtgagcgc   7740

ttcgatatcg gcgtcaggga cgccaccacc ggccagatga tgaaccggga atggtcagcc   7800

gccgaagtgc tccagaacac gccatggctc aagcggatga atgcccaggg caatgacgtg   7860

tatatcaggc ccgccgagca ggagcggcat ggtctggtgc tggtggacga cctcagcgag   7920

tttgacctgg atgacatgaa agccgagggc cgggagcctg ccctggtagt ggaaaccagc   7980

ccgaagaact atcaggcatg ggtcaaggtg gccgacgccg caggcggtga acttcggggg   8040

cagattgccc ggacgctggc cagcgagtac gacgccgacc cggccagcgc cgacagccgc   8100

cactatggcc gcttggcggg cttcaccaac cgcaaggaca agcacaccac ccgcgccggt   8160

tatcagccgt gggtgctgct gcgtgaatcc aagggcaaga ccgccaccgc tggcccggcg   8220

ctggtgcagc aggctggcca gcagatcgag caggcccagc ggcagcagga gaaggcccgc   8280

aggctggcca gcctcgaact gcccgagcgg cagcttagcc gccaccggcg cacggcgctg   8340

gacgagtacc gcagcgagat ggccgggctg gtcaagcgct tcggtgatga cctcagcaag   8400

tgcgacttta tcgccgcgca gaagctggcc agccggggcc gcagtgccga ggaaatcggc   8460

aaggccatgg ccgaggccag cccagcgctg gcagagcgca agcccggcca cgaagcggat   8520

tacatcgagc gcaccgtcag caaggtcatg ggtctgccca gcgtccagct tgcgcgggcc   8580

gagctggcac gggcaccggc accccgccag cgaggcatgg acaggggcgg gccagatttc   8640

agcatgtagt gcttgcgttg gtactcacgc ctgttatact atgagtactc acgcacagaa   8700

gggggtttta tggaatacga aaaaagcgct tcagggtcgg tctacctgat caaaagtgac   8760

aagggctatt ggttgcccgg tggctttggt tatacgtcaa acaaggccga ggctggccgc   8820

ttttcagtcg ctgatatggc cagccttaac cttgacggct gcaccttgtc cttgttccgc   8880

gaagacaagc ctttcggccc cggcaagttt ctcggtgact gatatgaaag accaaaagga   8940

caagcagacc ggcgacctgc tggccagccc tgacgctgta cgccaagcgc gatatgccga   9000

gcgcatgaag gccaaaggga tgcgtcagcg caagttctgg ctgaccgacg acgaatacga   9060

ggcgctgcgc gagtgcctgg aagaactcag agcggcgcag ggcgggggta gtgaccccgc   9120

cagcgcctaa ccaccaactg cctgcaaagg aggcaatcaa tggctaccca taagcctatc   9180

aatattctgg aggcgttcgc agcagcgccg ccaccgctgg actacgtttt gcccaacatg   9240

gtggccggta cggtcggggc gctggtgtcg cccggtggtg ccggtaaatc catgctggcc   9300

ctgcaactgg ccgcacagat tgcaggcggg ccggatctgc tggaggtggg cgaactgccc   9360

accggcccgg tgatctacct gcccgccgaa gacccgccca ccgccattca tcaccgcctg   9420

cacgcccttg gggcgcacct cagcgccgag gaacggcaag ccgtggctga cggcctgctg   9480

atccagccgc tgatcggcag cctgcccaac atcatgg                            9517


<210>  23
<211>  7183
<212>  DNA
<213>  artificial sequence

<220>
<223>  nucleotide sequence of plasmid pClikCM_delta_fruA

<400>  23
tcgagtagga gtaactcaag gtcaccgttt gcgttttgtt gccgaaggtg cgcaggcaca     60

gcaggcgatt gaagccatcg ctaaagaaat tgcggcgggc ttgggtgagc ctgtttccgc    120

cgttccgccg gcagaaccgg atactattga agtcgctaac ccggcgacac cggaagttga    180

gcaaccaaaa tccgacagta tcgaagcggt ttttgtgatt aataatgaaa acggtctgca    240

tgctcgtcct gcggcgactt tagtgaatga agtcaaaaaa tataatgcgt cggtggcggt    300

tcgtaattta gatcgcgacg gtgggttagt gagcgctaaa agcatgatga aaatcgttgc    360

attaggtgcg acaaaaggtt ctcgtctgca ttttgtcgcc accggtgaag aagctcaaca    420

agcgattgac ggtatcggtg cggcaatcgc ggcaggttta ggagaataaa caatggcaaa    480

agtggcaaca attacattaa acgccgccta tgatttggtc gggcgtttaa aacgcattga    540

attgggcgaa gtgaatacgg tggaaaccct cggtttattc cctgccggta aaggtatcaa    600

tgtggctaag gtgttgaatg acttagatgt tgaagtcgcg gtcggtggtt ttctcggtga    660

agataacgta ggcgatttcg agcatttatt ccaacaacaa ggtttgcagg ataaattcca    720

gcgggttgcc ggtaaaaccc gaataaacgt aaaaatcacc gaaacggacg cggatgtaac    780

ggatttgaat tttcttggtt atcaaatcag tgaacaggat tggcggaaat ttaccgcaga    840

ttctctcgct tattgtaaag aattcgacat cgttgccgtg tgtggcagtt tgccccgcgg    900

cgtaacggcg gatatgtttc aaagctggtt aagtcaatta catcaagcgg gtgtaaaagt    960

cgtactagat agtagtaatg ccgcattaac agcaggtctt aaagcaaatc cttggttagt   1020

gaaaccgaat caccgcgaat tagaagcctg ggtcggccat gagttaccga ctttgaaaga   1080

catcattgac gccgcaaaac aattaaaagc acaagggata gccaatgtta ttatttccat   1140

gggcgcaaac ggctcattat ggctaagtga taacggcgtg attttggctc agccgccgaa   1200

atgtgaaaac gtagtaagca cagtcggtgc cggcgattcg atggtcgcag gtttaattta   1260

tggttttgta aataatttat ctcaacaaga aacattggcg tttgcaagtg cggtatctgc   1320

cttcgccgtt tcacaaagta acgtaggtgt cagtgatcgc aagttgctcg acccaatctt   1380

agcaaatgta aaaatcacaa cgattgaagg ataagccgat gaatattttt cttacgcaat   1440

caccaaattt aggtcgtgca aaagcgtttt tattgcacca ggttttggct gccgcagtaa   1500

aacaatttcc taatcaagca taaagccttt gtttatctca aaacaaaggc tttttttata   1560

agtattccgc tttgcccgaa ctaatagaaa aattggcaga caaaagaagt gttcatagca   1620

caggaggaac aatatggatt tcaatgcaat tttaaatcaa gttttaagtg ccgctcagga   1680

aaccgttaag aaaacggcaa gcggcaatag cacaacggat aaagtggcaa aaatcggtgg   1740

cggtgcagcg gctatcggcg tgctttcgat gattttcggg cgcaccggcg gagcggggct   1800

tgcaaaatta ggctcgcttg ccgcattagg cagccttgct tatcaggctt accaggatta   1860

tcaacataaa caaagccaag ttgtaccggt tactgaaacg gaatttaccc aaagcgtaca   1920

acaatcggcg gaactcagca aagtgatttt gcaggcaatg attgcggcgg cggctgcaga   1980

cggcgcgatt tccgaccagg aacaacaagc gattttaagc caagccggcg atgatgcgga   2040

agtacagcag tggattcggc aagaaatgta tcaaccggca acagtgcggg aaatcgccca   2100

acaagtgggt gataatcagg cattagcatc acaggtgtat ctggcggcaa gaatggtttg   2160

cgccgattta gcacgcaaag aaattgtttt cttagcaaat ttagcgcaag cgttggggtt   2220

agatgaagcg cttgtagaac agttagaaaa acaggcgggt ttctgattta atcattccgc   2280

gatgtgcaaa gtgcggtcaa aaataacgat ttttttaccg cacttttgca tttgcaagac   2340

gtttcgaaaa tgcctgttct aacttctatt aaaacccttc ttctaaaatt ttctccaata   2400

actcaaatac cagcaacact cgtttgggag taatggtttg ataaggacgg tagaggtaaa   2460

ttccccaaat caaggtttcc tcattagaga agactacttg taactcgccg gaatcaagat   2520

aggatttaca gtcatgatac attatcggcg cgaaaatcct gccggataag accgccggta   2580

acaagctttt aatatcgctg gtgataaccg tcggcttagt tagaattatg ggttgttcac   2640

ccatcatcca gtcccatact ttgccggtct taggatttaa aatatagcct accggaaaat   2700

tggcggctaa atcaaaaacg tcttttggca atccggtttt agcgataaga ctaggtgctg   2760

ccacgatagg ctcttgtagg tcagtaatct ttttcgccac ccaatgatct tcgggcgtgc   2820

ggctgatgcg aataccgata tcaatttggt catccaccgc tttgagcgta tcgaaatccg   2880

tgcgccagtc aatctgaata tccggatagg gcgcaagtgc ggttagtaat cgcaataaaa   2940

ttttatccgc ataatcggaa ggcggtaacg taatccgcac taagccggaa aggctctctt   3000

ccgcatctag actccatagg ccgctttcct ggctttgctt ccagatgtat gctctcctcc   3060

ggagagtacc gtgactttat tttcggcaca aatacagggg tcgatggata aatacggcga   3120

tagtttcctg acggatgatc cgtatgtacc ggcggaagac aagctgcaaa cctgtcagat   3180

ggagattgat ttaatggcgg atgtgctgag agcaccgccc cgtgaatccg cagaactgat   3240

ccgctatgtg tttgcggatg attggccgga ataaataaag ccgggcttaa tacagattaa   3300

gcccgtatag ggtattatta ctgaatacca aacagcttac ggaggacgga atgttaccca   3360

ttgagacaac cagactgcct tctgattatt aatatttttc actattaatc agaaggaata   3420

accatgaatt ttacccggat tgacctgaat acctggaatc gcagggaaca ctttgccctt   3480

tatcgtcagc agattaaatg cggattcagc ctgaccacca aactcgatat taccgctttg   3540

cgtaccgcac tggcggagac aggttataag ttttatccgc tgatgattta cctgatctcc   3600

cgggctgtta atcagtttcc ggagttccgg atggcactga aagacaatga acttatttac   3660

tgggaccagt cagacccggt ctttactgtc tttcataaag aaaccgaaac attctctgca   3720

ctgtcctgcc gttattttcc ggatctcagt gagtttatgg caggttataa tgcggtaacg   3780

gcagaatatc agcatgatac cagattgttt ccgcagggaa atttaccgga gaatcacctg   3840

aatatatcat cattaccgtg ggtgagtttt gacgggattt aacctgaaca tcaccggaaa   3900

tgatgattat tttgccccgg tttttacgat ggcaaagttt cagcaggaag gtgaccgcgt   3960

attattacct gtttctgtac aggttcatca tgcagtctgt gatggctttc atgcagcacg   4020

gtttattaat acacttcagc tgatgtgtga taacatactg aaataaatta attaattctg   4080

tatttaagcc accgtatccg gcaggaatgg tggctttttt tttatatttt aaccgtaatc   4140

tgtaatttcg tttcagactg gttcaggatg agctcgcttg gactcctgtt gatagatcca   4200

gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt   4260

tttattggtg agaatccaag cactagcggc gcgccggccg gcccggtgtg aaataccgca   4320

cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc   4380

gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg   4440

gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa   4500

ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga   4560

cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag   4620

ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct   4680

taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg   4740

ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc   4800

ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt   4860

aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta   4920

tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac   4980

agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc   5040

ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat   5100

tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc   5160

tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt   5220

cacctagatc cttttaaagg ccggccgcgg ccgccatcgg cattttcttt tgcgttttta   5280

tttgttaact gttaattgtc cttgttcaag gatgctgtct ttgacaacag atgttttctt   5340

gcctttgatg ttcagcagga agctcggcgc aaacgttgat tgtttgtctg cgtagaatcc   5400

tctgtttgtc atatagcttg taatcacgac attgtttcct ttcgcttgag gtacagcgaa   5460

gtgtgagtaa gtaaaggtta catcgttagg atcaagatcc atttttaaca caaggccagt   5520

tttgttcagc ggcttgtatg ggccagttaa agaattagaa acataaccaa gcatgtaaat   5580
r
atcgttagac gtaatgccgt caatcgtcat ttttgatccg cgggagtcag tgaacaggta   5640

ccatttgccg ttcattttaa agacgttcgc gcgttcaatt tcatctgtta ctgtgttaga   5700

tgcaatcagc ggtttcatca cttttttcag tgtgtaatca tcgtttagct caatcatacc   5760

gagagcgccg tttgctaact cagccgtgcg ttttttatcg ctttgcagaa gtttttgact   5820

ttcttgacgg aagaatgatg tgcttttgcc atagtatgct ttgttaaata aagattcttc   5880

gccttggtag ccatcttcag ttccagtgtt tgcttcaaat actaagtatt tgtggccttt   5940

atcttctacg tagtgaggat ctctcagcgt atggttgtcg cctgagctgt agttgccttc   6000

atcgatgaac tgctgtacat tttgatacgt ttttccgtca ccgtcaaaga ttgatttata   6060

atcctctaca ccgttgatgt tcaaagagct gtctgatgct gatacgttaa cttgtgcagt   6120

tgtcagtgtt tgtttgccgt aatgtttacc ggagaaatca gtgtagaata aacggatttt   6180

tccgtcagat gtaaatgtgg ctgaacctga ccattcttgt gtttggtctt ttaggataga   6240

atcatttgca tcgaatttgt cgctgtcttt aaagacgcgg ccagcgtttt tccagctgtc   6300

aatagaagtt tcgccgactt tttgatagaa catgtaaatc gatgtgtcat ccgcattttt   6360

aggatctccg gctaatgcaa agacgatgtg gtagccgtga tagtttgcga cagtgccgtc   6420

agcgttttgt aatggccagc tgtcccaaac gtccaggcct tttgcagaag agatattttt   6480

aattgtggac gaatcaaatt cagaaacttg atatttttca tttttttgct gttcagggat   6540

ttgcagcata tcatggcgtg taatatggga aatgccgtat gtttccttat atggcttttg   6600

gttcgtttct ttcgcaaacg cttgagttgc gcctcctgcc agcagtgcgg tagtaaaggt   6660

taatactgtt gcttgttttg caaacttttt gatgttcatc gttcatgtct ccttttttat   6720

gtactgtgtt agcggtctgc ttcttccagc cctcctgttt gaagatggca agttagttac   6780

gcacaataaa aaaagaccta aaatatgtaa ggggtgacgc caaagtatac actttgccct   6840

ttacacattt taggtcttgc ctgctttatc agtaacaaac ccgcgcgatt tacttttcga   6900

cctcattcta ttagactctc gtttggattg caactggtct attttcctct tttgtttgat   6960

agaaaatcat aaaaggattt gcagactacg ggcctaaaga actaaaaaat ctatctgttt   7020

cttttcattc tctgtatttt ttatagtttc tgttgcatgg gcataaagtt gcctttttaa   7080

tcacaattca gaaaatatca taatatctca tttcactaaa taatagtgaa cggcaggtat   7140

atgtgatggg ttaaaaagga tcggcggccg ctcgatttaa atc                     7183


