                         SEQUENCE LISTING

<110>  Janssen Vaccines & Prevention B.V.
 
<120>  Methods and compositions for inducing protective immunity against
        RSV infection

<130>  0292 EP P00 PRI

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RSV preF2.2

<400>  1

Met Glu Leu Leu Ile Leu Lys Ala Asn Ala Ile Thr Thr Ile Leu Thr 
1               5                   10                  15      


Ala Val Thr Phe Cys Phe Ala Ser Gly Gln Asn Ile Thr Glu Glu Phe 
            20                  25                  30          


Tyr Gln Ser Thr Cys Ser Ala Val Ser Lys Gly Tyr Leu Ser Ala Leu 
        35                  40                  45              


Arg Thr Gly Trp Tyr Thr Ser Val Ile Thr Ile Glu Leu Ser Asn Ile 
    50                  55                  60                  


Lys Glu Ile Lys Cys Asn Gly Thr Asp Ala Lys Val Lys Leu Ile Lys 
65                  70                  75                  80  


Gln Glu Leu Asp Lys Tyr Lys Asn Ala Val Thr Glu Leu Gln Leu Leu 
                85                  90                  95      


Met Gln Ser Thr Pro Ala Thr Asn Asn Arg Ala Arg Arg Glu Leu Pro 
            100                 105                 110         


Arg Phe Met Asn Tyr Thr Leu Asn Asn Ala Lys Lys Thr Asn Val Thr 
        115                 120                 125             


Leu Ser Lys Lys Arg Lys Arg Arg Phe Leu Gly Phe Leu Leu Gly Val 
    130                 135                 140                 


Gly Ser Ala Ile Ala Ser Gly Val Ala Val Ser Lys Val Leu His Leu 
145                 150                 155                 160 


Glu Gly Glu Val Asn Lys Ile Lys Ser Ala Leu Leu Ser Thr Asn Lys 
                165                 170                 175     


Ala Val Val Ser Leu Ser Asn Gly Val Ser Val Leu Thr Ser Lys Val 
            180                 185                 190         


Leu Asp Leu Lys Asn Tyr Ile Asp Lys Gln Leu Leu Pro Ile Val Asn 
        195                 200                 205             


Lys Gln Ser Cys Ser Ile Pro Asn Ile Glu Thr Val Ile Glu Phe Gln 
    210                 215                 220                 


Gln Lys Asn Asn Arg Leu Leu Glu Ile Thr Arg Glu Phe Ser Val Asn 
225                 230                 235                 240 


Ala Gly Val Thr Thr Pro Val Ser Thr Tyr Met Leu Thr Asn Ser Glu 
                245                 250                 255     


Leu Leu Ser Leu Ile Asn Asp Met Pro Ile Thr Asn Asp Gln Lys Lys 
            260                 265                 270         


Leu Met Ser Asn Asn Val Gln Ile Val Arg Gln Gln Ser Tyr Ser Ile 
        275                 280                 285             


Met Ser Ile Ile Lys Glu Glu Val Leu Ala Tyr Val Val Gln Leu Pro 
    290                 295                 300                 


Leu Tyr Gly Val Ile Asp Thr Pro Cys Trp Lys Leu His Thr Ser Pro 
305                 310                 315                 320 


Leu Cys Thr Thr Asn Thr Lys Glu Gly Ser Asn Ile Cys Leu Thr Arg 
                325                 330                 335     


Thr Asp Arg Gly Trp Tyr Cys Asp Asn Ala Gly Ser Val Ser Phe Phe 
            340                 345                 350         


Pro Gln Ala Glu Thr Cys Lys Val Gln Ser Asn Arg Val Phe Cys Asp 
        355                 360                 365             


Thr Met Asn Ser Leu Thr Leu Pro Ser Glu Val Asn Leu Cys Asn Val 
    370                 375                 380                 


Asp Ile Phe Asn Pro Lys Tyr Asp Cys Lys Ile Met Thr Ser Lys Thr 
385                 390                 395                 400 


Asp Val Ser Ser Ser Val Ile Thr Ser Leu Gly Ala Ile Val Ser Cys 
                405                 410                 415     


Tyr Gly Lys Thr Lys Cys Thr Ala Ser Asn Lys Asn Arg Gly Ile Ile 
            420                 425                 430         


Lys Thr Phe Ser Asn Gly Cys Asp Tyr Val Ser Asn Lys Gly Val Asp 
        435                 440                 445             


Thr Val Ser Val Gly Asn Thr Leu Tyr Tyr Val Asn Lys Gln Glu Gly 
    450                 455                 460                 


Lys Ser Leu Tyr Val Lys Gly Glu Pro Ile Ile Asn Phe Tyr Asp Pro 
465                 470                 475                 480 


Leu Val Phe Pro Ser Asn Glu Phe Asp Ala Ser Ile Ser Gln Val Asn 
                485                 490                 495     


Glu Lys Ile Asn Gln Ser Leu Ala Phe Ile Arg Lys Ser Asp Glu Leu 
            500                 505                 510         


Leu His Asn Val Asn Ala Val Lys Ser Thr Thr Asn Ile Met Ile Thr 
        515                 520                 525             


Thr Ile Ile Ile Val Ile Ile Val Ile Leu Leu Ser Leu Ile Ala Val 
    530                 535                 540                 


Gly Leu Leu Leu Tyr Cys Lys Ala Arg Ser Thr Pro Val Thr Leu Ser 
545                 550                 555                 560 


Lys Asp Gln Leu Ser Gly Ile Asn Asn Ile Ala Phe Ser Asn 
                565                 570                 


<210>  2
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RSV preF2.1

<400>  2

Met Glu Leu Leu Ile Leu Lys Ala Asn Ala Ile Thr Thr Ile Leu Thr 
1               5                   10                  15      


Ala Val Thr Phe Cys Phe Ala Ser Gly Gln Asn Ile Thr Glu Glu Phe 
            20                  25                  30          


Tyr Gln Ser Thr Cys Ser Ala Val Ser Lys Gly Tyr Leu Gly Ala Leu 
        35                  40                  45              


Arg Thr Gly Trp Tyr Thr Ser Val Ile Thr Ile Glu Leu Ser Asn Ile 
    50                  55                  60                  


Lys Glu Ile Lys Cys Asn Gly Thr Asp Ala Lys Val Lys Leu Ile Lys 
65                  70                  75                  80  


Gln Glu Leu Asp Lys Tyr Lys Asn Ala Val Thr Glu Leu Gln Leu Leu 
                85                  90                  95      


Met Gln Ser Thr Pro Ala Thr Asn Asn Arg Ala Arg Arg Glu Leu Pro 
            100                 105                 110         


Arg Phe Met Asn Tyr Thr Leu Asn Asn Ala Lys Lys Thr Asn Val Thr 
        115                 120                 125             


Leu Ser Lys Lys Arg Lys Arg Arg Phe Leu Gly Phe Leu Leu Gly Val 
    130                 135                 140                 


Gly Ser Ala Ile Ala Ser Gly Val Ala Val Ser Lys Val Leu His Leu 
145                 150                 155                 160 


Glu Gly Glu Val Asn Lys Ile Lys Ser Ala Leu Leu Ser Thr Asn Lys 
                165                 170                 175     


Ala Val Val Ser Leu Ser Asn Gly Val Ser Val Leu Thr Ser Lys Val 
            180                 185                 190         


Leu Asp Leu Lys Asn Tyr Ile Asp Lys Gln Leu Leu Pro Ile Val Asn 
        195                 200                 205             


Lys Gln Ser Cys Ser Ile Pro Asn Ile Glu Thr Val Ile Glu Phe Gln 
    210                 215                 220                 


Gln Lys Asn Asn Arg Leu Leu Glu Ile Thr Arg Glu Phe Ser Val Asn 
225                 230                 235                 240 


Ala Gly Val Thr Thr Pro Val Ser Thr Tyr Met Leu Thr Asn Ser Glu 
                245                 250                 255     


Leu Leu Ser Leu Ile Asn Asp Met Pro Ile Thr Asn Asp Gln Lys Lys 
            260                 265                 270         


Leu Met Ser Asn Asn Val Gln Ile Val Arg Gln Gln Ser Tyr Ser Ile 
        275                 280                 285             


Met Ser Ile Ile Lys Glu Glu Val Leu Ala Tyr Val Val Gln Leu Pro 
    290                 295                 300                 


Leu Tyr Gly Val Ile Asp Thr Pro Cys Trp Lys Leu His Thr Ser Pro 
305                 310                 315                 320 


Leu Cys Thr Thr Asn Thr Lys Glu Gly Ser Asn Ile Cys Leu Thr Arg 
                325                 330                 335     


Thr Asp Arg Gly Trp Tyr Cys Asp Asn Ala Gly Ser Val Ser Phe Phe 
            340                 345                 350         


Pro Gln Ala Glu Thr Cys Lys Val Gln Ser Asn Arg Val Phe Cys Asp 
        355                 360                 365             


Thr Met Asn Ser Leu Thr Leu Pro Ser Glu Val Asn Leu Cys Asn Val 
    370                 375                 380                 


Asp Ile Phe Asn Pro Lys Tyr Asp Cys Lys Ile Met Thr Ser Lys Thr 
385                 390                 395                 400 


Asp Val Ser Ser Ser Val Ile Thr Ser Leu Gly Ala Ile Val Ser Cys 
                405                 410                 415     


Tyr Gly Lys Thr Lys Cys Thr Ala Ser Asn Lys Asn Arg Gly Ile Ile 
            420                 425                 430         


Lys Thr Phe Ser Asn Gly Cys Asp Tyr Val Ser Asn Lys Gly Val Asp 
        435                 440                 445             


Thr Val Ser Val Gly Asn Thr Leu Tyr Tyr Val Asn Lys Gln Glu Gly 
    450                 455                 460                 


Lys Ser Leu Tyr Val Lys Gly Glu Pro Ile Ile Asn Phe Tyr Asp Pro 
465                 470                 475                 480 


Leu Val Phe Pro Ser Asp Glu Phe Asp Ala Ser Ile Ser Gln Val Asn 
                485                 490                 495     


Glu Lys Ile Asn Gln Ser Leu Ala Phe Ile Arg Lys Ser Asp Glu Leu 
            500                 505                 510         


Leu His Asn Val Asn Ala Val Lys Ser Thr Thr Asn Ile Met Ile Thr 
        515                 520                 525             


Thr Ile Ile Ile Val Ile Ile Val Ile Leu Leu Ser Leu Ile Ala Val 
    530                 535                 540                 


Gly Leu Leu Leu Tyr Cys Lys Ala Arg Ser Thr Pro Val Thr Leu Ser 
545                 550                 555                 560 


Lys Asp Gln Leu Ser Gly Ile Asn Asn Ile Ala Phe Ser Asn 
                565                 570                 


<210>  3
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimized nucleic acid encoding the RSV F pre-F2.2

<400>  3
atggagctgc tgatcctgaa ggccaacgcc atcaccacca tcctgaccgc cgtgaccttc       60

tgcttcgcca gcggccagaa catcaccgag gagttctacc agagcacctg cagcgccgtg      120

agcaagggct acctgagcgc cctgagaacc ggctggtaca ccagcgtgat caccatcgag      180

ctgagcaaca tcaaggagat caagtgcaac ggcaccgacg ccaaggtgaa gctgatcaag      240

caggagctgg acaagtacaa gaacgccgtg accgagctgc agctgctgat gcagagcacc      300

cccgccacca acaacagagc cagaagagag ctgcccagat tcatgaacta caccctgaac      360

aacgccaaga agaccaacgt gaccctgagc aagaagagaa agagaagatt cctgggcttc      420

ctgctgggcg tgggcagcgc catcgccagc ggcgtggccg tgagcaaggt gctgcacctg      480

gagggcgagg tgaacaagat caagagcgcc ctgctgagca ccaacaaggc cgtggtgagc      540

ctgagcaacg gcgtgagcgt gctgaccagc aaggtgctgg acctgaagaa ctacatcgac      600

aagcagctgc tgcccatcgt gaacaagcag agctgcagca tccccaacat cgagaccgtg      660

atcgagttcc agcagaagaa caacagactg ctggagatca ccagagagtt cagcgtgaac      720

gccggcgtga ccacccccgt gagcacctac atgctgacca acagcgagct gctgagcctg      780

atcaacgaca tgcccatcac caacgaccag aagaagctga tgagcaacaa cgtgcagatc      840

gtgagacagc agagctacag catcatgagc atcatcaagg aggaggtgct ggcctacgtg      900

gtgcagctgc ccctgtacgg cgtgatcgac accccctgct ggaagctgca caccagcccc      960

ctgtgcacca ccaacaccaa ggagggcagc aacatctgcc tgaccagaac cgacagaggc     1020

tggtactgcg acaacgccgg cagcgtgagc ttcttccccc aggccgagac ctgcaaggtg     1080

cagagcaaca gagtgttctg cgacaccatg aacagcctga ccctgcccag cgaggtgaac     1140

ctgtgcaacg tggacatctt caaccccaag tacgactgca agatcatgac cagcaagacc     1200

gacgtgagca gcagcgtgat caccagcctg ggcgccatcg tgagctgcta cggcaagacc     1260

aagtgcaccg ccagcaacaa gaacagaggc atcatcaaga ccttcagcaa cggctgcgac     1320

tacgtgagca acaagggcgt ggacaccgtg agcgtgggca acaccctgta ctacgtgaac     1380

aagcaggagg gcaagagcct gtacgtgaag ggcgagccca tcatcaactt ctacgacccc     1440

ctggtgttcc ccagcaacga gttcgacgcc agcatcagcc aggtgaacga gaagatcaac     1500

cagagcctgg ccttcatcag aaagagcgac gagctgctgc acaacgtgaa cgccgtgaag     1560

agcaccacca acatcatgat caccaccatc atcatcgtga tcatcgtgat cctgctgagc     1620

ctgatcgccg tgggcctgct gctgtactgc aaggccagaa gcacccccgt gaccctgagc     1680

aaggaccagc tgagcggcat caacaacatc gccttcagca actga                     1725


<210>  4
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimized nucleic acid encoding the RSV F pre-F2.1 
       pre-fusion protein

<400>  4
atggagctgc tgatcctgaa ggccaacgcc atcaccacca tcctgaccgc cgtgaccttc       60

tgcttcgcca gcggccagaa catcaccgag gagttctacc agagcacctg cagcgccgtg      120

agcaagggct acctgggcgc cctgagaacc ggctggtaca ccagcgtgat caccatcgag      180

ctgagcaaca tcaaggagat caagtgcaac ggcaccgacg ccaaggtgaa gctgatcaag      240

caggagctgg acaagtacaa gaacgccgtg accgagctgc agctgctgat gcagagcacc      300

cccgccacca acaacagagc cagaagagag ctgcccagat tcatgaacta caccctgaac      360

aacgccaaga agaccaacgt gaccctgagc aagaagagaa agagaagatt cctgggcttc      420

ctgctgggcg tgggcagcgc catcgccagc ggcgtggccg tgagcaaggt gctgcacctg      480

gagggcgagg tgaacaagat caagagcgcc ctgctgagca ccaacaaggc cgtggtgagc      540

ctgagcaacg gcgtgagcgt gctgaccagc aaggtgctgg acctgaagaa ctacatcgac      600

aagcagctgc tgcccatcgt gaacaagcag agctgcagca tccccaacat cgagaccgtg      660

atcgagttcc agcagaagaa caacagactg ctggagatca ccagagagtt cagcgtgaac      720

gccggcgtga ccacccccgt gagcacctac atgctgacca acagcgagct gctgagcctg      780

atcaacgaca tgcccatcac caacgaccag aagaagctga tgagcaacaa cgtgcagatc      840

gtgagacagc agagctacag catcatgagc atcatcaagg aggaggtgct ggcctacgtg      900

gtgcagctgc ccctgtacgg cgtgatcgac accccctgct ggaagctgca caccagcccc      960

ctgtgcacca ccaacaccaa ggagggcagc aacatctgcc tgaccagaac cgacagaggc     1020

tggtactgcg acaacgccgg cagcgtgagc ttcttccccc aggccgagac ctgcaaggtg     1080

cagagcaaca gagtgttctg cgacaccatg aacagcctga ccctgcccag cgaggtgaac     1140

ctgtgcaacg tggacatctt caaccccaag tacgactgca agatcatgac cagcaagacc     1200

gacgtgagca gcagcgtgat caccagcctg ggcgccatcg tgagctgcta cggcaagacc     1260

aagtgcaccg ccagcaacaa gaacagaggc atcatcaaga ccttcagcaa cggctgcgac     1320

tacgtgagca acaagggcgt ggacaccgtg agcgtgggca acaccctgta ctacgtgaac     1380

aagcaggagg gcaagagcct gtacgtgaag ggcgagccca tcatcaactt ctacgacccc     1440

ctggtgttcc ccagcgacga gttcgacgcc agcatcagcc aggtgaacga gaagatcaac     1500

cagagcctgg ccttcatcag aaagagcgac gagctgctgc acaacgtgaa cgccgtgaag     1560

agcaccacca acatcatgat caccaccatc atcatcgtga tcatcgtgat cctgctgagc     1620

ctgatcgccg tgggcctgct gctgtactgc aaggccagaa gcacccccgt gaccctgagc     1680

aaggaccagc tgagcggcat caacaacatc gccttcagca actga                     1725


