                         SEQUENCE LISTING

<110>  Wellstat ImmunoTherapeutics Corporation
 
<120>  Enveloped Virus Resistant to Complement Inactivation for the 
       Treatment of Cancer

<130>  21003-PCT

<150>  US 62/504,120
<151>  2017-05-10

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  6606
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mammalian cell expression construct.


<220>
<221>  misc_feature
<222>  (1064)..(1165)
<223>  Code for secretory signal peptide

<220>
<221>  misc_feature
<222>  (1166)..(2119)
<223>  Code for SCR of CD55

<220>
<221>  misc_feature
<222>  (2120)..(2164)
<223>  Code for (G4S1)3 linker

<220>
<221>  misc_feature
<222>  (2165)..(2227)
<223>  Code for CD8 transmembrane domain

<220>
<221>  misc_feature
<222>  (2228)..(2260)
<223>  Code for truncated CD8 intracellular domain

<400>  1
tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta       60

ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc      120

aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg      180

gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc      240

gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat      300

agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc      360

ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga      420

cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg      480

gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac      540

caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt      600

caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc      660

cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc      720

tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat      780

tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc      840

gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa      900

actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac      960

tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta     1020

aggctagagt acttaatacg actcactata ggctagcgcc accatgacag tggccagacc     1080

ttctgtgcct gccgccctgc ctctgctggg agaactgcct agactgctgc tgctggtgct     1140

gctgtgtctg cctgccgtgt ggggcgattg tggcctgcct cccgatgtgc ctaatgccca     1200

gcctgccctg gaaggcagaa ccagcttccc cgaggacacc gtgatcacct acaagtgcga     1260

ggaatccttc gtgaagatcc ccggcgagaa ggatagcgtg atctgcctga agggcagcca     1320

gtggagcgac atcgaagagt tctgcaacag atcctgcgag gtgcccaccc ggctgaatag     1380

cgcctctctg aagcagccct acatcaccca gaactacttc cctgtgggca ccgtggtgga     1440

atacgagtgc agacccggct acagaagaga gccctccctg agccctaagc tgacctgcct     1500

gcagaacctg aagtggtcca ccgccgtgga gttctgtaaa aagaagtcct gccccaaccc     1560

tggcgagatc cggaacggcc agattgatgt gcctggcggc atcctgttcg gcgccacaat     1620

cagcttcagc tgcaacaccg gctacaagct gttcggcagc acctccagct tttgcctgat     1680

cagcggcagc agcgtgcagt ggagtgaccc tctgcctgag tgcagagaga tctactgccc     1740

tgccccccct cagatcgaca acggcatcat tcagggcgag cgggaccact acggctacag     1800

gcagagcgtg acctacgcct gcaacaaggg cttcaccatg atcggcgagc acagcatcta     1860

ctgcaccgtg aacaacgacg agggcgagtg gagcggccca ccccctgagt gtagaggcaa     1920

gagcctgacc agcaaggtgc cccccaccgt gcagaaaccc accaccgtga atgtgcctac     1980

caccgaggtg tccccaacca gccagaaaac aaccaccaag accaccaccc ccaacgccca     2040

ggccaccaga tctacccctg tgtccaggac caccaagcac ttccacgaga caacccctaa     2100

caagggcagc ggcacaaccg gtggcggagg atctggcggc ggaggaagcg gagggggagg     2160

atccatctat atctgggccc ctctggccgg cacctgtggc gtgctgctgc tgtctctcgt     2220

gatcaccctg tactgcaacc accggaaccg gcggagagtg tgatgagaat tcacgcgtgg     2280

tacctctaga gtcgaccctc tagggcggcc aattccgccc ctctccctcc ccccccccta     2340

acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt     2400

ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga     2460

cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg     2520

tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt     2580

gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat     2640

aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg     2700

aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg     2760

taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt     2820

cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa     2880

cacgatgata agcttgccac aacccgggat aattcctgca gccaatatgg gatcggccat     2940

tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta     3000

tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca     3060

ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga     3120

cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga     3180

cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct     3240

cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg     3300

gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga     3360

gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca     3420

tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga     3480

tgatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg     3540

cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc     3600

gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt     3660

gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga     3720

gttcttctga ggggatcaat tctgggcggc ctcgagaata aacaatcatt attttcattg     3780

gatctgtgtg ttggtttttt gtgtgggctt gggggagggg gaggccagaa tgactccaag     3840

agctacagga aggcaggtca gagaccccac tggacaaaca gtggctggac tctgcaccat     3900

aacacacaat caacagggga gtgagctgga tcgagctgct cgagatccgg gctggcgtaa     3960

tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg     4020

gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc     4080

gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc     4140

acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt     4200

agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg     4260

ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt     4320

ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta     4380

taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt     4440

aacgcgaatt ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta     4500

cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg     4560

ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt     4620

gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc     4680

agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat     4740

ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg     4800

gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc     4860

tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta     4920

ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg     4980

ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg     5040

gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac     5100

gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg     5160

acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt     5220

actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg     5280

ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac     5340

cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt     5400

gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag     5460

caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc     5520

aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc     5580

ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta     5640

tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg     5700

ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga     5760

ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac     5820

ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa     5880

tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat     5940

cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc     6000

taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg     6060

gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc     6120

acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg     6180

ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg     6240

ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa     6300

cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg     6360

aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga     6420

gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct     6480

gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca     6540

gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atggctcgac     6600

agatct                                                                6606


<210>  2
<211>  399
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein


<220>
<221>  SIGNAL
<222>  (1)..(34)
<223>  Secretory signal peptide

<220>
<221>  PEPTIDE
<222>  (35)..(352)
<223>  SCR of CD55

<220>
<221>  MISC_FEATURE
<222>  (353)..(367)
<223>  (G4S1)3 linker

<220>
<221>  TRANSMEM
<222>  (368)..(388)
<223>  CD8 transmembrane domain

<220>
<221>  DOMAIN
<222>  (389)..(399)
<223>  Truncated CD8 intracellular domain

<400>  2

Met Thr Val Ala Arg Pro Ser Val Pro Ala Ala Leu Pro Leu Leu Gly 
1               5                   10                  15      


Glu Leu Pro Arg Leu Leu Leu Leu Val Leu Leu Cys Leu Pro Ala Val 
            20                  25                  30          


Trp Gly Asp Cys Gly Leu Pro Pro Asp Val Pro Asn Ala Gln Pro Ala 
        35                  40                  45              


Leu Glu Gly Arg Thr Ser Phe Pro Glu Asp Thr Val Ile Thr Tyr Lys 
    50                  55                  60                  


Cys Glu Glu Ser Phe Val Lys Ile Pro Gly Glu Lys Asp Ser Val Ile 
65                  70                  75                  80  


Cys Leu Lys Gly Ser Gln Trp Ser Asp Ile Glu Glu Phe Cys Asn Arg 
                85                  90                  95      


Ser Cys Glu Val Pro Thr Arg Leu Asn Ser Ala Ser Leu Lys Gln Pro 
            100                 105                 110         


Tyr Ile Thr Gln Asn Tyr Phe Pro Val Gly Thr Val Val Glu Tyr Glu 
        115                 120                 125             


Cys Arg Pro Gly Tyr Arg Arg Glu Pro Ser Leu Ser Pro Lys Leu Thr 
    130                 135                 140                 


Cys Leu Gln Asn Leu Lys Trp Ser Thr Ala Val Glu Phe Cys Lys Lys 
145                 150                 155                 160 


Lys Ser Cys Pro Asn Pro Gly Glu Ile Arg Asn Gly Gln Ile Asp Val 
                165                 170                 175     


Pro Gly Gly Ile Leu Phe Gly Ala Thr Ile Ser Phe Ser Cys Asn Thr 
            180                 185                 190         


Gly Tyr Lys Leu Phe Gly Ser Thr Ser Ser Phe Cys Leu Ile Ser Gly 
        195                 200                 205             


Ser Ser Val Gln Trp Ser Asp Pro Leu Pro Glu Cys Arg Glu Ile Tyr 
    210                 215                 220                 


Cys Pro Ala Pro Pro Gln Ile Asp Asn Gly Ile Ile Gln Gly Glu Arg 
225                 230                 235                 240 


Asp His Tyr Gly Tyr Arg Gln Ser Val Thr Tyr Ala Cys Asn Lys Gly 
                245                 250                 255     


Phe Thr Met Ile Gly Glu His Ser Ile Tyr Cys Thr Val Asn Asn Asp 
            260                 265                 270         


Glu Gly Glu Trp Ser Gly Pro Pro Pro Glu Cys Arg Gly Lys Ser Leu 
        275                 280                 285             


Thr Ser Lys Val Pro Pro Thr Val Gln Lys Pro Thr Thr Val Asn Val 
    290                 295                 300                 


Pro Thr Thr Glu Val Ser Pro Thr Ser Gln Lys Thr Thr Thr Lys Thr 
305                 310                 315                 320 


Thr Thr Pro Asn Ala Gln Ala Thr Arg Ser Thr Pro Val Ser Arg Thr 
                325                 330                 335     


Thr Lys His Phe His Glu Thr Thr Pro Asn Lys Gly Ser Gly Thr Thr 
            340                 345                 350         


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ile 
        355                 360                 365             


Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser 
    370                 375                 380                 


Leu Val Ile Thr Leu Tyr Cys Asn His Arg Asn Arg Arg Arg Val 
385                 390                 395                 


<210>  3
<211>  365
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mature fusion protein (after cleavage of signal peptide).


<220>
<221>  PEPTIDE
<222>  (1)..(318)
<223>  SCR of CD55

<220>
<221>  MISC_FEATURE
<222>  (319)..(333)
<223>  (G4S1)3 linker

<220>
<221>  TRANSMEM
<222>  (334)..(354)
<223>  CD8 transmembrane domain

<220>
<221>  DOMAIN
<222>  (355)..(365)
<223>  Trunated CD8 intracellular domain

<400>  3

Asp Cys Gly Leu Pro Pro Asp Val Pro Asn Ala Gln Pro Ala Leu Glu 
1               5                   10                  15      


Gly Arg Thr Ser Phe Pro Glu Asp Thr Val Ile Thr Tyr Lys Cys Glu 
            20                  25                  30          


Glu Ser Phe Val Lys Ile Pro Gly Glu Lys Asp Ser Val Ile Cys Leu 
        35                  40                  45              


Lys Gly Ser Gln Trp Ser Asp Ile Glu Glu Phe Cys Asn Arg Ser Cys 
    50                  55                  60                  


Glu Val Pro Thr Arg Leu Asn Ser Ala Ser Leu Lys Gln Pro Tyr Ile 
65                  70                  75                  80  


Thr Gln Asn Tyr Phe Pro Val Gly Thr Val Val Glu Tyr Glu Cys Arg 
                85                  90                  95      


Pro Gly Tyr Arg Arg Glu Pro Ser Leu Ser Pro Lys Leu Thr Cys Leu 
            100                 105                 110         


Gln Asn Leu Lys Trp Ser Thr Ala Val Glu Phe Cys Lys Lys Lys Ser 
        115                 120                 125             


Cys Pro Asn Pro Gly Glu Ile Arg Asn Gly Gln Ile Asp Val Pro Gly 
    130                 135                 140                 


Gly Ile Leu Phe Gly Ala Thr Ile Ser Phe Ser Cys Asn Thr Gly Tyr 
145                 150                 155                 160 


Lys Leu Phe Gly Ser Thr Ser Ser Phe Cys Leu Ile Ser Gly Ser Ser 
                165                 170                 175     


Val Gln Trp Ser Asp Pro Leu Pro Glu Cys Arg Glu Ile Tyr Cys Pro 
            180                 185                 190         


Ala Pro Pro Gln Ile Asp Asn Gly Ile Ile Gln Gly Glu Arg Asp His 
        195                 200                 205             


Tyr Gly Tyr Arg Gln Ser Val Thr Tyr Ala Cys Asn Lys Gly Phe Thr 
    210                 215                 220                 


Met Ile Gly Glu His Ser Ile Tyr Cys Thr Val Asn Asn Asp Glu Gly 
225                 230                 235                 240 


Glu Trp Ser Gly Pro Pro Pro Glu Cys Arg Gly Lys Ser Leu Thr Ser 
                245                 250                 255     


Lys Val Pro Pro Thr Val Gln Lys Pro Thr Thr Val Asn Val Pro Thr 
            260                 265                 270         


Thr Glu Val Ser Pro Thr Ser Gln Lys Thr Thr Thr Lys Thr Thr Thr 
        275                 280                 285             


Pro Asn Ala Gln Ala Thr Arg Ser Thr Pro Val Ser Arg Thr Thr Lys 
    290                 295                 300                 


His Phe His Glu Thr Thr Pro Asn Lys Gly Ser Gly Thr Thr Gly Gly 
305                 310                 315                 320 


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ile Tyr Ile 
                325                 330                 335     


Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val 
            340                 345                 350         


Ile Thr Leu Tyr Cys Asn His Arg Asn Arg Arg Arg Val 
        355                 360                 365 


