                         SEQUENCE LISTING

<110>  VIB VZW
       UNIVERSITEIT GENT
 
<120>  GLYCOSYLATION OF IMMUNOGLOBULIN DOMAINS

<130>  NC/NbGly/586

<150>  EP 17170661.7
<151>  2017-05-11

<160>  31    

<170>  PatentIn version 3.5

<210>  1
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  nanobody

<400>  1

Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu 
1               5                   10                  15      


Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Asn Tyr Trp Met 
            20                  25                  30          


Tyr Trp Val Arg Arg Ala Pro Gly Lys Gly Leu Glu Trp Val Ser Met 
        35                  40                  45              


Ile Asn Pro Gly Gly Ile Ile Thr Lys Tyr Ala Glu Ser Val Lys Gly 
    50                  55                  60                  


Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr Leu Gln 
65                  70                  75                  80  


Met Asn Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ala Lys 
                85                  90                  95      


Asp Trp Ala Thr Gly Leu Ala Lys Lys Gly Gln Gly Thr Gln Val Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  2
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR1

<400>  2

Gly Phe Thr Phe Asn Asn Tyr Trp 
1               5               


<210>  3
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR2

<400>  3

Ile Asn Pro Gly Gly Ile Ile Thr Lys 
1               5                   


<210>  4
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR3

<400>  4

Ala Lys Asp Trp Ala Thr Gly Leu Ala 
1               5                   


<210>  5
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FR1

<400>  5

Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu 
1               5                   10                  15      


Arg Leu Ser Cys Ala Ala Ser 
            20              


<210>  6
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FR2

<400>  6

Met Tyr Trp Val Arg Arg Ala Pro Gly Lys Gly Leu Glu Trp Val Ser 
1               5                   10                  15      


Met 
    


<210>  7
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FR3

<400>  7

Tyr Ala Glu Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala 
1               5                   10                  15      


Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Thr Ser Glu Asp Thr 
            20                  25                  30          


Ala Val Tyr Tyr Cys 
        35          


<210>  8
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FR4

<400>  8

Lys Lys Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
1               5                   10          


<210>  9
<211>  121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nanobody

<400>  9

Gln Val Gln Leu Val Glu Ser Gly Gly Ala Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Pro Val Asn Arg Tyr 
            20                  25                  30          


Ser Met Arg Trp Tyr Arg Gln Ala Pro Gly Lys Glu Arg Glu Trp Val 
        35                  40                  45              


Ala Gly Met Ser Ser Ala Gly Asp Arg Ser Ser Tyr Glu Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ala Arg Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Asn Val Asn Val Gly Phe Glu Tyr Trp Gly Gln Gly Thr Gln Val Thr 
            100                 105                 110         


Val Ser Ser His His His His His His 
        115                 120     


<210>  10
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR1

<400>  10

Gly Phe Pro Val Asn Arg Tyr Ser 
1               5               


<210>  11
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR2

<400>  11

Met Ser Ser Ala Gly Asp Arg Ser Ser 
1               5                   


<210>  12
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR3

<400>  12

Asn Val Asn Val Gly Phe Glu 
1               5           


<210>  13
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FR1

<400>  13

Gln Val Gln Leu Val Glu Ser Gly Gly Ala Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser 
            20                  25  


<210>  14
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FR2

<400>  14

Met Arg Trp Tyr Arg Gln Ala Pro Gly Lys Glu Arg Glu Trp Val Ala 
1               5                   10                  15      


Gly 
    


<210>  15
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FR3

<400>  15

Tyr Glu Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ala 
1               5                   10                  15      


Arg Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr 
            20                  25                  30          


Ala Val Tyr Tyr Cys 
        35          


<210>  16
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FR4

<400>  16

Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
1               5                   10          


<210>  17
<211>  129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nanobody

<400>  17

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Ser Ile Phe Ser Ile Asn 
            20                  25                  30          


Ala Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Val 
        35                  40                  45              


Ala Ala Ile Ser Ser Gly Gly Arg Thr Asn Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val His Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn 
                85                  90                  95      


Val Gly Ser Trp Gly Phe Arg Ser His Ser Tyr Leu Ser Gly Ser Ser 
            100                 105                 110         


Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His His His His 
        115                 120                 125             


His 
    


<210>  18
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR1

<400>  18

Gly Ser Ile Phe Ser Ile Asn Ala 
1               5               


<210>  19
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR2

<400>  19

Ile Ser Ser Gly Gly Arg Thr Asn 
1               5               


<210>  20
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR3

<400>  20

Asn Val Gly Ser Trp Gly Phe Arg Ser His Ser Tyr Leu Ser Gly Ser 
1               5                   10                  15      


<210>  21
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FR1

<400>  21

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Ala Ser 
            20                  25  


<210>  22
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FR2

<400>  22

Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Val Ala 
1               5                   10                  15      


Ala 
    


<210>  23
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FR3

<400>  23

Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala 
1               5                   10                  15      


Lys Asn Thr Val His Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr 
            20                  25                  30          


Ala Val Tyr Tyr Cys 
        35          


<210>  24
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FR4

<400>  24

Ser Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
1               5                   10          


<210>  25
<211>  135
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nanobody

<400>  25

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Leu Asp Tyr Tyr 
            20                  25                  30          


Tyr Ile Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Ala Val 
        35                  40                  45              


Ser Cys Ile Ser Gly Ser Ser Gly Ser Thr Tyr Tyr Pro Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Thr Ile Arg Ser Ser Ser Trp Gly Gly Cys Val His Tyr Gly Met 
            100                 105                 110         


Asp Tyr Trp Gly Lys Gly Thr Gln Val Thr Val Ser Ser Gly Ser His 
        115                 120                 125             


His His His His His His His 
    130                 135 


<210>  26
<211>  135
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nanobody

<400>  26

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Leu Asp Tyr Tyr 
            20                  25                  30          


Tyr Ile Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Gly Val 
        35                  40                  45              


Ser Cys Ile Ser Ser Ser His Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Thr Val Ala Val Ala His Phe Arg Gly Cys Gly Val Asp Gly Met 
            100                 105                 110         


Asp Tyr Trp Gly Lys Gly Thr Gln Val Thr Val Ser Ser Gly Ser His 
        115                 120                 125             


His His His His His His His 
    130                 135 


<210>  27
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  27

Gly Gly Gly Ser Ser 
1               5   


<210>  28
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial N-terminal glyco-tag

<400>  28

Gln Ala Asp Asp Ala Asn Ala Thr Val Gln Leu Val Glu Ser Gly Gly 
1               5                   10                  15      


Ala 
    


<210>  29
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  part of wildtype GBP

<400>  29

Gln Val Gln Leu Val Glu Ser Gly Gly Ala 
1               5                   10  


<210>  30
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C-terminal glyco-HIS-tag

<400>  30

Val Ser Ser Leu Gln Ala Ala Ala Ala Ala Ala Asn Ala Thr Val Ala 
1               5                   10                  15      


Ala Ala Ser Gly Asp Val Trp Asp Ile His His His His His His 
            20                  25                  30      


<210>  31
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Part of wildtype GBP

<400>  31

Val Ser Ser His His His His His His 
1               5                   


