                         SEQUENCE LISTING

<110>  Zentrum fr Forschungsfrderung in der Pdiatrie GmbH
 
<120>  Concept for the Treatment of Monogenetic Disorders

<130>  17.6081.7

<160>  28    

<170>  PatentIn version 3.5

<210>  1
<211>  211
<212>  DNA
<213>  Homo sapiens


<220>
<221>  EF1-alpha Promoter
<222>  (1)..(211)

<400>  1
ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg       60

ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt      120

gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca      180

gtagtcgccg tgaacgttct ttttcgcaac g                                     211


<210>  2
<211>  4374
<212>  DNA
<213>  Homo sapiens


<220>
<221>  FVIII
<222>  (1)..(4374)
<223>  factor FVIII SQ with deleted B-domain

<220>
<221>  FVIII_wt
<222>  (1)..(4374)
<223>  factor FVIII SQ with deleted B-domain, wt sequence

<400>  2
atgcaaatag agctctccac ctgcttcttt ctgtgccttt tgcgattctg ctttagtgcc       60

accagaagat actacctggg tgcagtggaa ctgtcatggg actatatgca aagtgatctc      120

ggtgagctgc ctgtggacgc aagatttcct cctagagtgc caaaatcttt tccattcaac      180

acctcagtcg tgtacaaaaa gactctgttt gtagaattca cggatcacct tttcaacatc      240

gctaagccaa ggccaccctg gatgggtctg ctaggtccta ccatccaggc tgaggtttat      300

gatacagtgg tcattacact taagaacatg gcttcccatc ctgtcagtct tcatgctgtt      360

ggtgtatcct actggaaagc ttctgaggga gctgaatatg atgatcagac cagtcaaagg      420

gagaaagaag atgataaagt cttccctggt ggaagccata catatgtctg gcaggtcctg      480

aaagagaatg gtccaatggc ctctgaccca ctgtgcctta cctactcata tctttctcat      540

gtggacctgg taaaagactt gaattcaggc ctcattggag ccctactagt atgtagagaa      600

gggagtctgg ccaaggaaaa gacacagacc ttgcacaaat ttatactact ttttgctgta      660

tttgatgaag ggaaaagttg gcactcagaa acaaagaact ccttgatgca ggatagggat      720

gctgcatctg ctcgggcctg gcctaaaatg cacacagtca atggttatgt aaacaggtct      780

ctgccaggtc tgattggatg ccacaggaaa tcagtctatt ggcatgtgat tggaatgggc      840

accactcctg aagtgcactc aatattcctc gaaggtcaca catttcttgt gaggaaccat      900

cgccaggcgt ccttggaaat ctcgccaata actttcctta ctgctcaaac actcttgatg      960

gaccttggac agtttctact gttttgtcat atctcttccc accaacatga tggcatggaa     1020

gcttatgtca aagtagacag ctgtccagag gaaccccaac tacgaatgaa aaataatgaa     1080

gaagcggaag actatgatga tgatcttact gattctgaaa tggatgtggt caggtttgat     1140

gatgacaact ctccttcctt tatccaaatt cgctcagttg ccaagaagca tcctaaaact     1200

tgggtacatt acattgctgc tgaagaggag gactgggact atgctccctt agtcctcgcc     1260

cccgatgaca gaagttataa aagtcaatat ttgaacaatg gccctcagcg gattggtagg     1320

aagtacaaaa aagtccgatt tatggcatac acagatgaaa cctttaagac tcgtgaagct     1380

attcagcatg aatcaggaat cttgggacct ttactttatg gggaagttgg agacacactg     1440

ttgattatat ttaagaatca agcaagcaga ccatataaca tctaccctca cggaatcact     1500

gatgtccgtc ctttgtattc aaggagatta ccaaaaggtg taaaacattt gaaggatttt     1560

ccaattctgc caggagaaat attcaaatat aaatggacag tgactgtaga agatgggcca     1620

actaaatcag atcctcggtg cctgacccgc tattactcta gtttcgttaa tatggagaga     1680

gatctagctt caggactcat tggccctctc ctcatctgct acaaagaatc tgtagatcaa     1740

agaggaaacc agataatgtc agacaagagg aatgtcatcc tgttttctgt atttgatgag     1800

aaccgaagct ggtacctcac agagaatata caacgctttc tccccaatcc agctggagtg     1860

cagcttgagg atccagagtt ccaagcctcc aacatcatgc acagcatcaa tggctatgtt     1920

tttgatagtt tgcagttgtc agtttgtttg catgaggtgg catactggta cattctaagc     1980

attggagcac agactgactt cctttctgtc ttcttctctg gatatacctt caaacacaaa     2040

atggtctatg aagacacact caccctattc ccattctcag gagaaactgt cttcatgtcg     2100

atggaaaacc caggtctatg gattctgggg tgccacaact cagactttcg gaacagaggc     2160

atgaccgcct tactgaaggt ttctagttgt gacaagaaca ctggtgatta ttacgaggac     2220

agttatgaag atatttcagc atacttgctg agtaaaaaca atgccattga accaagaagc     2280

ttctcccaga atccaccagt cttaaaaaga caccagagag aaataactcg tactactctt     2340

cagtcagatc aagaggaaat tgactatgat gataccatat cagttgaaat gaagaaggaa     2400

gattttgaca tttatgatga ggatgaaaat cagagccccc gcagctttca aaagaaaaca     2460

cgacactatt ttattgctgc agtggagagg ctctgggatt atgggatgag tagctcccca     2520

catgttctaa gaaacagggc tcagagtggc agtgtccctc agttcaagaa agttgttttc     2580

caggaattta ctgatggctc ctttactcag cccttatacc gtggagaact aaatgaacat     2640

ttgggactcc tggggccata tataagagca gaagttgaag ataatatcat ggtaactttc     2700

agaaatcagg cctctcgtcc ctattccttc tattctagcc ttatttctta tgaggaagat     2760

cagaggcaag gagcagaacc tagaaaaaac tttgtcaagc ctaatgaaac caaaacttac     2820

ttttggaaag tgcaacatca tatggcaccc actaaagatg agtttgactg caaagcctgg     2880

gcttatttct ctgatgttga cctggaaaaa gatgtgcact caggcctgat tggacccctt     2940

ctggtctgcc acactaacac actgaaccct gctcatggga gacaagtgac agtacaggaa     3000

tttgctctgt ttttcaccat ctttgatgag accaaaagct ggtacttcac tgaaaatatg     3060

gaaagaaact gcagggctcc ctgcaatatc cagatggaag atcccacttt taaagagaat     3120

tatcgcttcc atgcaatcaa tggctacata atggatacac tacctggctt agtaatggct     3180

caggatcaaa ggattcgatg gtatctgctc agcatgggca gcaatgaaaa catccattct     3240

attcatttca gtggacatgt gttcactgta cgaaaaaaag aggagtataa aatggcactg     3300

tacaatctct atccaggtgt ttttgagaca gtggaaatgt taccatccaa agctggaatt     3360

tggcgggtgg aatgccttat tggcgagcat ctacatgctg ggatgagcac actttttctg     3420

gtgtacagca ataagtgtca gactcccctg ggaatggctt ctggacacat tagagatttt     3480

cagattacag cttcaggaca atatggacag tgggccccaa agctggccag acttcattat     3540

tccggatcaa tcaatgcctg gagcaccaag gagccctttt cttggatcaa ggtggatctg     3600

ttggcaccaa tgattattca cggcatcaag acccagggtg cccgtcagaa gttctccagc     3660

ctctacatct ctcagtttat catcatgtat agtcttgatg ggaagaagtg gcagacttat     3720

cgaggaaatt ccactggaac cttaatggtc ttctttggca atgtggattc atctgggata     3780

aaacacaata tttttaaccc tccaattatt gctcgataca tccgtttgca cccaactcat     3840

tatagcattc gcagcactct tcgcatggag ttgatgggct gtgatttaaa tagttgcagc     3900

atgccattgg gaatggagag taaagcaata tcagatgcac agattactgc ttcatcctac     3960

tttaccaata tgtttgccac ctggtctcct tcaaaagctc gacttcacct ccaagggagg     4020

agtaatgcct ggagacctca ggtgaataat ccaaaagagt ggctgcaagt ggacttccag     4080

aagacaatga aagtcacagg agtaactact cagggagtaa aatctctgct taccagcatg     4140

tatgtgaagg agttcctcat ctccagcagt caagatggcc atcagtggac tctctttttt     4200

cagaatggca aagtaaaggt ttttcaggga aatcaagact ccttcacacc tgtggtgaac     4260

tctctagacc caccgttact gactcgctac cttcgaattc acccccagag ttgggtgcac     4320

cagattgccc tgaggatgga ggttctgggc tgcgaggcac aggacctcta ctga           4374


<210>  3
<211>  1457
<212>  PRT
<213>  Homo sapiens


<220>
<221>  FVIII
<222>  (1)..(1457)
<223>  Factor FVIII SQ with deleted B-domain

<400>  3

Met Gln Ile Glu Leu Ser Thr Cys Phe Phe Leu Cys Leu Leu Arg Phe 
1               5                   10                  15      


Cys Phe Ser Ala Thr Arg Arg Tyr Tyr Leu Gly Ala Val Glu Leu Ser 
            20                  25                  30          


Trp Asp Tyr Met Gln Ser Asp Leu Gly Glu Leu Pro Val Asp Ala Arg 
        35                  40                  45              


Phe Pro Pro Arg Val Pro Lys Ser Phe Pro Phe Asn Thr Ser Val Val 
    50                  55                  60                  


Tyr Lys Lys Thr Leu Phe Val Glu Phe Thr Asp His Leu Phe Asn Ile 
65                  70                  75                  80  


Ala Lys Pro Arg Pro Pro Trp Met Gly Leu Leu Gly Pro Thr Ile Gln 
                85                  90                  95      


Ala Glu Val Tyr Asp Thr Val Val Ile Thr Leu Lys Asn Met Ala Ser 
            100                 105                 110         


His Pro Val Ser Leu His Ala Val Gly Val Ser Tyr Trp Lys Ala Ser 
        115                 120                 125             


Glu Gly Ala Glu Tyr Asp Asp Gln Thr Ser Gln Arg Glu Lys Glu Asp 
    130                 135                 140                 


Asp Lys Val Phe Pro Gly Gly Ser His Thr Tyr Val Trp Gln Val Leu 
145                 150                 155                 160 


Lys Glu Asn Gly Pro Met Ala Ser Asp Pro Leu Cys Leu Thr Tyr Ser 
                165                 170                 175     


Tyr Leu Ser His Val Asp Leu Val Lys Asp Leu Asn Ser Gly Leu Ile 
            180                 185                 190         


Gly Ala Leu Leu Val Cys Arg Glu Gly Ser Leu Ala Lys Glu Lys Thr 
        195                 200                 205             


Gln Thr Leu His Lys Phe Ile Leu Leu Phe Ala Val Phe Asp Glu Gly 
    210                 215                 220                 


Lys Ser Trp His Ser Glu Thr Lys Asn Ser Leu Met Gln Asp Arg Asp 
225                 230                 235                 240 


Ala Ala Ser Ala Arg Ala Trp Pro Lys Met His Thr Val Asn Gly Tyr 
                245                 250                 255     


Val Asn Arg Ser Leu Pro Gly Leu Ile Gly Cys His Arg Lys Ser Val 
            260                 265                 270         


Tyr Trp His Val Ile Gly Met Gly Thr Thr Pro Glu Val His Ser Ile 
        275                 280                 285             


Phe Leu Glu Gly His Thr Phe Leu Val Arg Asn His Arg Gln Ala Ser 
    290                 295                 300                 


Leu Glu Ile Ser Pro Ile Thr Phe Leu Thr Ala Gln Thr Leu Leu Met 
305                 310                 315                 320 


Asp Leu Gly Gln Phe Leu Leu Phe Cys His Ile Ser Ser His Gln His 
                325                 330                 335     


Asp Gly Met Glu Ala Tyr Val Lys Val Asp Ser Cys Pro Glu Glu Pro 
            340                 345                 350         


Gln Leu Arg Met Lys Asn Asn Glu Glu Ala Glu Asp Tyr Asp Asp Asp 
        355                 360                 365             


Leu Thr Asp Ser Glu Met Asp Val Val Arg Phe Asp Asp Asp Asn Ser 
    370                 375                 380                 


Pro Ser Phe Ile Gln Ile Arg Ser Val Ala Lys Lys His Pro Lys Thr 
385                 390                 395                 400 


Trp Val His Tyr Ile Ala Ala Glu Glu Glu Asp Trp Asp Tyr Ala Pro 
                405                 410                 415     


Leu Val Leu Ala Pro Asp Asp Arg Ser Tyr Lys Ser Gln Tyr Leu Asn 
            420                 425                 430         


Asn Gly Pro Gln Arg Ile Gly Arg Lys Tyr Lys Lys Val Arg Phe Met 
        435                 440                 445             


Ala Tyr Thr Asp Glu Thr Phe Lys Thr Arg Glu Ala Ile Gln His Glu 
    450                 455                 460                 


Ser Gly Ile Leu Gly Pro Leu Leu Tyr Gly Glu Val Gly Asp Thr Leu 
465                 470                 475                 480 


Leu Ile Ile Phe Lys Asn Gln Ala Ser Arg Pro Tyr Asn Ile Tyr Pro 
                485                 490                 495     


His Gly Ile Thr Asp Val Arg Pro Leu Tyr Ser Arg Arg Leu Pro Lys 
            500                 505                 510         


Gly Val Lys His Leu Lys Asp Phe Pro Ile Leu Pro Gly Glu Ile Phe 
        515                 520                 525             


Lys Tyr Lys Trp Thr Val Thr Val Glu Asp Gly Pro Thr Lys Ser Asp 
    530                 535                 540                 


Pro Arg Cys Leu Thr Arg Tyr Tyr Ser Ser Phe Val Asn Met Glu Arg 
545                 550                 555                 560 


Asp Leu Ala Ser Gly Leu Ile Gly Pro Leu Leu Ile Cys Tyr Lys Glu 
                565                 570                 575     


Ser Val Asp Gln Arg Gly Asn Gln Ile Met Ser Asp Lys Arg Asn Val 
            580                 585                 590         


Ile Leu Phe Ser Val Phe Asp Glu Asn Arg Ser Trp Tyr Leu Thr Glu 
        595                 600                 605             


Asn Ile Gln Arg Phe Leu Pro Asn Pro Ala Gly Val Gln Leu Glu Asp 
    610                 615                 620                 


Pro Glu Phe Gln Ala Ser Asn Ile Met His Ser Ile Asn Gly Tyr Val 
625                 630                 635                 640 


Phe Asp Ser Leu Gln Leu Ser Val Cys Leu His Glu Val Ala Tyr Trp 
                645                 650                 655     


Tyr Ile Leu Ser Ile Gly Ala Gln Thr Asp Phe Leu Ser Val Phe Phe 
            660                 665                 670         


Ser Gly Tyr Thr Phe Lys His Lys Met Val Tyr Glu Asp Thr Leu Thr 
        675                 680                 685             


Leu Phe Pro Phe Ser Gly Glu Thr Val Phe Met Ser Met Glu Asn Pro 
    690                 695                 700                 


Gly Leu Trp Ile Leu Gly Cys His Asn Ser Asp Phe Arg Asn Arg Gly 
705                 710                 715                 720 


Met Thr Ala Leu Leu Lys Val Ser Ser Cys Asp Lys Asn Thr Gly Asp 
                725                 730                 735     


Tyr Tyr Glu Asp Ser Tyr Glu Asp Ile Ser Ala Tyr Leu Leu Ser Lys 
            740                 745                 750         


Asn Asn Ala Ile Glu Pro Arg Ser Phe Ser Gln Asn Pro Pro Val Leu 
        755                 760                 765             


Lys Arg His Gln Arg Glu Ile Thr Arg Thr Thr Leu Gln Ser Asp Gln 
    770                 775                 780                 


Glu Glu Ile Asp Tyr Asp Asp Thr Ile Ser Val Glu Met Lys Lys Glu 
785                 790                 795                 800 


Asp Phe Asp Ile Tyr Asp Glu Asp Glu Asn Gln Ser Pro Arg Ser Phe 
                805                 810                 815     


Gln Lys Lys Thr Arg His Tyr Phe Ile Ala Ala Val Glu Arg Leu Trp 
            820                 825                 830         


Asp Tyr Gly Met Ser Ser Ser Pro His Val Leu Arg Asn Arg Ala Gln 
        835                 840                 845             


Ser Gly Ser Val Pro Gln Phe Lys Lys Val Val Phe Gln Glu Phe Thr 
    850                 855                 860                 


Asp Gly Ser Phe Thr Gln Pro Leu Tyr Arg Gly Glu Leu Asn Glu His 
865                 870                 875                 880 


Leu Gly Leu Leu Gly Pro Tyr Ile Arg Ala Glu Val Glu Asp Asn Ile 
                885                 890                 895     


Met Val Thr Phe Arg Asn Gln Ala Ser Arg Pro Tyr Ser Phe Tyr Ser 
            900                 905                 910         


Ser Leu Ile Ser Tyr Glu Glu Asp Gln Arg Gln Gly Ala Glu Pro Arg 
        915                 920                 925             


Lys Asn Phe Val Lys Pro Asn Glu Thr Lys Thr Tyr Phe Trp Lys Val 
    930                 935                 940                 


Gln His His Met Ala Pro Thr Lys Asp Glu Phe Asp Cys Lys Ala Trp 
945                 950                 955                 960 


Ala Tyr Phe Ser Asp Val Asp Leu Glu Lys Asp Val His Ser Gly Leu 
                965                 970                 975     


Ile Gly Pro Leu Leu Val Cys His Thr Asn Thr Leu Asn Pro Ala His 
            980                 985                 990         


Gly Arg Gln Val Thr Val Gln Glu  Phe Ala Leu Phe Phe  Thr Ile Phe 
        995                 1000                 1005             


Asp Glu  Thr Lys Ser Trp Tyr  Phe Thr Glu Asn Met  Glu Arg Asn 
    1010                 1015                 1020             


Cys Arg  Ala Pro Cys Asn Ile  Gln Met Glu Asp Pro  Thr Phe Lys 
    1025                 1030                 1035             


Glu Asn  Tyr Arg Phe His Ala  Ile Asn Gly Tyr Ile  Met Asp Thr 
    1040                 1045                 1050             


Leu Pro  Gly Leu Val Met Ala  Gln Asp Gln Arg Ile  Arg Trp Tyr 
    1055                 1060                 1065             


Leu Leu  Ser Met Gly Ser Asn  Glu Asn Ile His Ser  Ile His Phe 
    1070                 1075                 1080             


Ser Gly  His Val Phe Thr Val  Arg Lys Lys Glu Glu  Tyr Lys Met 
    1085                 1090                 1095             


Ala Leu  Tyr Asn Leu Tyr Pro  Gly Val Phe Glu Thr  Val Glu Met 
    1100                 1105                 1110             


Leu Pro  Ser Lys Ala Gly Ile  Trp Arg Val Glu Cys  Leu Ile Gly 
    1115                 1120                 1125             


Glu His  Leu His Ala Gly Met  Ser Thr Leu Phe Leu  Val Tyr Ser 
    1130                 1135                 1140             


Asn Lys  Cys Gln Thr Pro Leu  Gly Met Ala Ser Gly  His Ile Arg 
    1145                 1150                 1155             


Asp Phe  Gln Ile Thr Ala Ser  Gly Gln Tyr Gly Gln  Trp Ala Pro 
    1160                 1165                 1170             


Lys Leu  Ala Arg Leu His Tyr  Ser Gly Ser Ile Asn  Ala Trp Ser 
    1175                 1180                 1185             


Thr Lys  Glu Pro Phe Ser Trp  Ile Lys Val Asp Leu  Leu Ala Pro 
    1190                 1195                 1200             


Met Ile  Ile His Gly Ile Lys  Thr Gln Gly Ala Arg  Gln Lys Phe 
    1205                 1210                 1215             


Ser Ser  Leu Tyr Ile Ser Gln  Phe Ile Ile Met Tyr  Ser Leu Asp 
    1220                 1225                 1230             


Gly Lys  Lys Trp Gln Thr Tyr  Arg Gly Asn Ser Thr  Gly Thr Leu 
    1235                 1240                 1245             


Met Val  Phe Phe Gly Asn Val  Asp Ser Ser Gly Ile  Lys His Asn 
    1250                 1255                 1260             


Ile Phe  Asn Pro Pro Ile Ile  Ala Arg Tyr Ile Arg  Leu His Pro 
    1265                 1270                 1275             


Thr His  Tyr Ser Ile Arg Ser  Thr Leu Arg Met Glu  Leu Met Gly 
    1280                 1285                 1290             


Cys Asp  Leu Asn Ser Cys Ser  Met Pro Leu Gly Met  Glu Ser Lys 
    1295                 1300                 1305             


Ala Ile  Ser Asp Ala Gln Ile  Thr Ala Ser Ser Tyr  Phe Thr Asn 
    1310                 1315                 1320             


Met Phe  Ala Thr Trp Ser Pro  Ser Lys Ala Arg Leu  His Leu Gln 
    1325                 1330                 1335             


Gly Arg  Ser Asn Ala Trp Arg  Pro Gln Val Asn Asn  Pro Lys Glu 
    1340                 1345                 1350             


Trp Leu  Gln Val Asp Phe Gln  Lys Thr Met Lys Val  Thr Gly Val 
    1355                 1360                 1365             


Thr Thr  Gln Gly Val Lys Ser  Leu Leu Thr Ser Met  Tyr Val Lys 
    1370                 1375                 1380             


Glu Phe  Leu Ile Ser Ser Ser  Gln Asp Gly His Gln  Trp Thr Leu 
    1385                 1390                 1395             


Phe Phe  Gln Asn Gly Lys Val  Lys Val Phe Gln Gly  Asn Gln Asp 
    1400                 1405                 1410             


Ser Phe  Thr Pro Val Val Asn  Ser Leu Asp Pro Pro  Leu Leu Thr 
    1415                 1420                 1425             


Arg Tyr  Leu Arg Ile His Pro  Gln Ser Trp Val His  Gln Ile Ala 
    1430                 1435                 1440             


Leu Arg  Met Glu Val Leu Gly  Cys Glu Ala Gln Asp  Leu Tyr 
    1445                 1450                 1455         


<210>  4
<211>  1386
<212>  DNA
<213>  Homo sapiens


<220>
<221>  FIX
<222>  (1)..(1386)
<223>  Factor FIX, codon optimized

<400>  4
atgcaacgcg tgaacatgat tatggccgag agccctggcc tgatcaccat ctgcctgctg       60

ggctacctgc tgagcgccga gtgcaccgtg tttctggacc acgagaacgc caacaagatc      120

ctgaaccggc ccaagcggta caacagcggc aagctggaag agttcgtgca gggcaacctg      180

gaacgcgagt gcatggaaga gaagtgcagc ttcgaagagg ccagagaggt gttcgagaac      240

accgagcgga ccaccgagtt ctggaagcag tacgtggacg gcgaccagtg cgagagcaac      300

ccctgtctga atggcggcag ctgcaaggac gacatcaaca gctacgagtg ctggtgcccc      360

ttcggcttcg agggcaagaa ctgcgagctg gacgtgacct gcaacatcaa gaacggcaga      420

tgcgagcagt tctgcaagaa cagcgccgac aacaaggtcg tgtgctcctg caccgagggc      480

tacagactgg ccgagaacca gaagtcctgc gagcccgccg tgcctttccc atgtggaaga      540

gtgtccgtgt cccagaccag caagctgacc agagccgaga cagtgttccc cgacgtggac      600

tacgtgaaca gcaccgaggc cgagacaatc ctggacaaca tcacccagag cacccagtcc      660

ttcaacgact tcaccagagt cgtgggcggc gaggatgcca agcctggaca gttcccgtgg      720

caggtggtgc tgaacggaaa ggtggacgcc ttttgcggcg gcagcatcgt gaacgagaag      780

tggatcgtga cagccgccca ctgcgtggaa accggcgtga agattacagt ggtggccggc      840

gagcacaaca tcgaggaaac cgagcacaca gagcagaaac ggaacgtgat cagaatcatc      900

ccccaccaca actacaacgc cgccatcaac aagtacaacc acgatatcgc cctgctggaa      960

ctggacgagc ccctggtgct gaatagctac gtgaccccca tctgtatcgc cgacaaagag     1020

tacaccaaca tctttctgaa gttcggcagc ggctacgtgt ccggctgggg cagagtgttt     1080

cacaagggca gatccgctct ggtgctgcag tacctgagag tgcctctggt ggaccgggcc     1140

acctgtctga gaagcaccaa gttcaccatc tacaacaaca tgttctgcgc cggctttcac     1200

gagggcggca gagatagctg tcagggcgat tctggcggcc ctcacgtgac agaggtggaa     1260

ggcaccagct ttctgaccgg catcatcagc tggggcgagg aatgcgccat gaaggggaag     1320

tacggcatct acaccaaggt gtccagatac gtgaactgga tcaaagaaaa gaccaagctg     1380

acatga                                                                1386


<210>  5
<211>  1386
<212>  DNA
<213>  Homo sapiens


<220>
<221>  FIX
<222>  (1)..(1386)
<223>  Factor FIX padua, codon optimized

<400>  5
atgcaacgcg tgaacatgat tatggccgag agccctggcc tgatcaccat ctgcctgctg       60

ggctacctgc tgagcgccga gtgcaccgtg tttctggacc acgagaacgc caacaagatc      120

ctgaaccggc ccaagcggta caacagcggc aagctggaag agttcgtgca gggcaacctg      180

gaacgcgagt gcatggaaga gaagtgcagc ttcgaagagg ccagagaggt gttcgagaac      240

accgagcgga ccaccgagtt ctggaagcag tacgtggacg gcgaccagtg cgagagcaac      300

ccctgtctga atggcggcag ctgcaaggac gacatcaaca gctacgagtg ctggtgcccc      360

ttcggcttcg agggcaagaa ctgcgagctg gacgtgacct gcaacatcaa gaacggcaga      420

tgcgagcagt tctgcaagaa cagcgccgac aacaaggtcg tgtgctcctg caccgagggc      480

tacagactgg ccgagaacca gaagtcctgc gagcccgccg tgcctttccc atgtggaaga      540

gtgtccgtgt cccagaccag caagctgacc agagccgaga cagtgttccc cgacgtggac      600

tacgtgaaca gcaccgaggc cgagacaatc ctggacaaca tcacccagag cacccagtcc      660

ttcaacgact tcaccagagt cgtgggcggc gaggatgcca agcctggaca gttcccgtgg      720

caggtggtgc tgaacggaaa ggtggacgcc ttttgcggcg gcagcatcgt gaacgagaag      780

tggatcgtga cagccgccca ctgcgtggaa accggcgtga agattacagt ggtggccggc      840

gagcacaaca tcgaggaaac cgagcacaca gagcagaaac ggaacgtgat cagaatcatc      900

ccccaccaca actacaacgc cgccatcaac aagtacaacc acgatatcgc cctgctggaa      960

ctggacgagc ccctggtgct gaatagctac gtgaccccca tctgtatcgc cgacaaagag     1020

tacaccaaca tctttctgaa gttcggcagc ggctacgtgt ccggctgggg cagagtgttt     1080

cacaagggca gatccgctct ggtgctgcag tacctgagag tgcctctggt ggaccgggcc     1140

acctgtctgc tgagcaccaa gttcaccatc tacaacaaca tgttctgcgc cggctttcac     1200

gagggcggca gagatagctg tcagggcgat tctggcggcc ctcacgtgac agaggtggaa     1260

ggcaccagct ttctgaccgg catcatcagc tggggcgagg aatgcgccat gaaggggaag     1320

tacggcatct acaccaaggt gtccagatac gtgaactgga tcaaagaaaa gaccaagctt     1380

acatga                                                                1386


<210>  6
<211>  3195
<212>  DNA
<213>  Homo sapiens


<220>
<221>  FIX_albumin
<222>  (1)..(3195)
<223>  fusion of FIX and albumin, codon optimized

<400>  6
atgcaacgcg tgaacatgat tatggccgag agccctggcc tgatcaccat ctgcctgctg       60

ggctacctgc tgagcgccga gtgcaccgtg tttctggacc acgagaacgc caacaagatc      120

ctgaaccggc ccaagcggta caacagcggc aagctggaag agttcgtgca gggcaacctg      180

gaacgcgagt gcatggaaga gaagtgcagc ttcgaagagg ccagagaggt gttcgagaac      240

accgagcgga ccaccgagtt ctggaagcag tacgtggacg gcgaccagtg cgagagcaac      300

ccctgtctga atggcggcag ctgcaaggac gacatcaaca gctacgagtg ctggtgcccc      360

ttcggcttcg agggcaagaa ctgcgagctg gacgtgacct gcaacatcaa gaacggcaga      420

tgcgagcagt tctgcaagaa cagcgccgac aacaaggtcg tgtgctcctg caccgagggc      480

tacagactgg ccgagaacca gaagtcctgc gagcccgccg tgcctttccc atgtggaaga      540

gtgtccgtgt cccagaccag caagctgacc agagccgaga cagtgttccc cgacgtggac      600

tacgtgaaca gcaccgaggc cgagacaatc ctggacaaca tcacccagag cacccagtcc      660

ttcaacgact tcaccagagt cgtgggcggc gaggatgcca agcctggaca gttcccgtgg      720

caggtggtgc tgaacggaaa ggtggacgcc ttttgcggcg gcagcatcgt gaacgagaag      780

tggatcgtga cagccgccca ctgcgtggaa accggcgtga agattacagt ggtggccggc      840

gagcacaaca tcgaggaaac cgagcacaca gagcagaaac ggaacgtgat cagaatcatc      900

ccccaccaca actacaacgc cgccatcaac aagtacaacc acgatatcgc cctgctggaa      960

ctggacgagc ccctggtgct gaatagctac gtgaccccca tctgtatcgc cgacaaagag     1020

tacaccaaca tctttctgaa gttcggcagc ggctacgtgt ccggctgggg cagagtgttt     1080

cacaagggca gatccgctct ggtgctgcag tacctgagag tgcctctggt ggaccgggcc     1140

acctgtctga gaagcaccaa gttcaccatc tacaacaaca tgttctgcgc cggctttcac     1200

gagggcggca gagatagctg tcagggcgat tctggcggcc ctcacgtgac agaggtggaa     1260

ggcaccagct ttctgaccgg catcatcagc tggggcgagg aatgcgccat gaaggggaag     1320

tacggcatct acaccaaggt gtccagatac gtgaactgga tcaaagaaaa gaccaagctt     1380

acacccgtgt ctcagacctc taagctgacc cgggctgaaa ctgtgtttcc agatgtggac     1440

gcccacaaga gcgaggtggc ccacagattc aaggacctgg gagaagagaa cttcaaggct     1500

ctggtgctga tcgccttcgc tcagtacctc cagcagtgcc cattcgagga ccatgtgaag     1560

ctggtcaacg aagtgaccga gttcgccaag acctgcgtgg ccgatgagtc cgccgagaac     1620

tgtgataaga gcctgcacac cctgttcggc gacaagctgt gtacagtggc cacactgaga     1680

gaaacctacg gcgagatggc cgactgctgc gccaaacaag agcccgagag aaacgagtgc     1740

ttcctccagc acaaggacga taaccccaac ctgcctagac tcgtgcggcc cgaagtggat     1800

gtcatgtgca ccgccttcca cgacaacgag gaaaccttcc tgaagaagta cctgtacgag     1860

atcgccagac ggcaccccta cttttacgcc cctgagctgc tgttctttgc caagagatac     1920

aaggccgcct tcaccgagtg ttgccaggcc gctgataagg ccgcttgtct gctgcctaag     1980

ctggatgagc tgcgcgacga gggcaaagcc tcttctgcca agcagagact gaagtgcgcc     2040

agcctccaga agtttggcga gagagccttt aaggcctggg ccgtcgctag actgagccag     2100

agatttccca aggccgagtt tgccgaggtg tccaagctgg ttaccgacct gaccaaggtg     2160

cacacagagt gctgtcacgg cgatctgctg gaatgcgccg acgatagagc cgatctggcc     2220

aagtacatct gcgagaatca ggacagcatc agctccaagc tgaaagaatg ctgcgagaag     2280

cccctgctcg aaaagagcca ctgtatcgct gaggtggaaa acgacgagat gcccgccgat     2340

ctgccttctc tggccgccga ttttgtggaa agcaaggacg tgtgcaagaa ttacgccgag     2400

gccaaggatg tgttcctggg catgtttctg tatgagtacg cccgcagaca ccccgactac     2460

tctgttgtgc tgctgctgag actggccaaa acctacgaga ctaccctgga aaagtgctgt     2520

gccgccgctg atcctcacga gtgttacgcc aaagtgttcg acgagttcaa gccactggtg     2580

gaagaacccc agaacctgat caaacagaac tgcgaactgt tcgagcagct gggcgagtac     2640

aagttccaga acgccctgct cgtgcggtac accaagaagg tgccccaggt ttcaacccct     2700

acactggttg aggtgtcccg gaacctgggc aaagtgggca gcaagtgttg caagcaccct     2760

gaggccaaga gaatgccctg cgccgaggat tacctgagcg tcgtgctgaa tcagctgtgc     2820

gtgctgcacg agaaaacccc tgtgtccgac agagtgacca agtgctgtac cgagagcctg     2880

gtcaacagac ggccttgctt tagcgccctt gaggtggacg agacatacgt gcccaaagag     2940

ttcaacgccg agacattcac cttccacgcc gacatctgta ccctgagcga gaaagagcgg     3000

cagatcaaga aacagaccgc tctggtggaa ctggtcaagc acaagcccaa ggccaccaaa     3060

gaacaactga aggccgtgat ggacgacttc gccgcctttg tcgagaagtg ctgcaaggcc     3120

gatgacaaag agacatgctt cgccgaagag ggaaagaaac tggtggccgc ctctcaagcc     3180

gctctgggac tttaa                                                      3195


<210>  7
<211>  3195
<212>  DNA
<213>  Homo sapiens


<220>
<221>  FIX_albumin
<222>  (1)..(3195)
<223>  fusion of FIX padua and albumin, codon optimized

<400>  7
atgcaacgcg tgaacatgat tatggccgag agccctggcc tgatcaccat ctgcctgctg       60

ggctacctgc tgagcgccga gtgcaccgtg tttctggacc acgagaacgc caacaagatc      120

ctgaaccggc ccaagcggta caacagcggc aagctggaag agttcgtgca gggcaacctg      180

gaacgcgagt gcatggaaga gaagtgcagc ttcgaagagg ccagagaggt gttcgagaac      240

accgagcgga ccaccgagtt ctggaagcag tacgtggacg gcgaccagtg cgagagcaac      300

ccctgtctga atggcggcag ctgcaaggac gacatcaaca gctacgagtg ctggtgcccc      360

ttcggcttcg agggcaagaa ctgcgagctg gacgtgacct gcaacatcaa gaacggcaga      420

tgcgagcagt tctgcaagaa cagcgccgac aacaaggtcg tgtgctcctg caccgagggc      480

tacagactgg ccgagaacca gaagtcctgc gagcccgccg tgcctttccc atgtggaaga      540

gtgtccgtgt cccagaccag caagctgacc agagccgaga cagtgttccc cgacgtggac      600

tacgtgaaca gcaccgaggc cgagacaatc ctggacaaca tcacccagag cacccagtcc      660

ttcaacgact tcaccagagt cgtgggcggc gaggatgcca agcctggaca gttcccgtgg      720

caggtggtgc tgaacggaaa ggtggacgcc ttttgcggcg gcagcatcgt gaacgagaag      780

tggatcgtga cagccgccca ctgcgtggaa accggcgtga agattacagt ggtggccggc      840

gagcacaaca tcgaggaaac cgagcacaca gagcagaaac ggaacgtgat cagaatcatc      900

ccccaccaca actacaacgc cgccatcaac aagtacaacc acgatatcgc cctgctggaa      960

ctggacgagc ccctggtgct gaatagctac gtgaccccca tctgtatcgc cgacaaagag     1020

tacaccaaca tctttctgaa gttcggcagc ggctacgtgt ccggctgggg cagagtgttt     1080

cacaagggca gatccgctct ggtgctgcag tacctgagag tgcctctggt ggaccgggcc     1140

acctgtctgc tgagcaccaa gttcaccatc tacaacaaca tgttctgcgc cggctttcac     1200

gagggcggca gagatagctg tcagggcgat tctggcggcc ctcacgtgac agaggtggaa     1260

ggcaccagct ttctgaccgg catcatcagc tggggcgagg aatgcgccat gaaggggaag     1320

tacggcatct acaccaaggt gtccagatac gtgaactgga tcaaagaaaa gaccaagctt     1380

acacccgtgt ctcagacctc taagctgacc cgggctgaaa ctgtgtttcc agatgtggac     1440

gcccacaaga gcgaggtggc ccacagattc aaggacctgg gagaagagaa cttcaaggct     1500

ctggtgctga tcgccttcgc tcagtacctc cagcagtgcc cattcgagga ccatgtgaag     1560

ctggtcaacg aagtgaccga gttcgccaag acctgcgtgg ccgatgagtc cgccgagaac     1620

tgtgataaga gcctgcacac cctgttcggc gacaagctgt gtacagtggc cacactgaga     1680

gaaacctacg gcgagatggc cgactgctgc gccaaacaag agcccgagag aaacgagtgc     1740

ttcctccagc acaaggacga taaccccaac ctgcctagac tcgtgcggcc cgaagtggat     1800

gtcatgtgca ccgccttcca cgacaacgag gaaaccttcc tgaagaagta cctgtacgag     1860

atcgccagac ggcaccccta cttttacgcc cctgagctgc tgttctttgc caagagatac     1920

aaggccgcct tcaccgagtg ttgccaggcc gctgataagg ccgcttgtct gctgcctaag     1980

ctggatgagc tgcgcgacga gggcaaagcc tcttctgcca agcagagact gaagtgcgcc     2040

agcctccaga agtttggcga gagagccttt aaggcctggg ccgtcgctag actgagccag     2100

agatttccca aggccgagtt tgccgaggtg tccaagctgg ttaccgacct gaccaaggtg     2160

cacacagagt gctgtcacgg cgatctgctg gaatgcgccg acgatagagc cgatctggcc     2220

aagtacatct gcgagaatca ggacagcatc agctccaagc tgaaagaatg ctgcgagaag     2280

cccctgctcg aaaagagcca ctgtatcgct gaggtggaaa acgacgagat gcccgccgat     2340

ctgccttctc tggccgccga ttttgtggaa agcaaggacg tgtgcaagaa ttacgccgag     2400

gccaaggatg tgttcctggg catgtttctg tatgagtacg cccgcagaca ccccgactac     2460

tctgttgtgc tgctgctgag actggccaaa acctacgaga ctaccctgga aaagtgctgt     2520

gccgccgctg atcctcacga gtgttacgcc aaagtgttcg acgagttcaa gccactggtg     2580

gaagaacccc agaacctgat caaacagaac tgcgaactgt tcgagcagct gggcgagtac     2640

aagttccaga acgccctgct cgtgcggtac accaagaagg tgccccaggt ttcaacccct     2700

acactggttg aggtgtcccg gaacctgggc aaagtgggca gcaagtgttg caagcaccct     2760

gaggccaaga gaatgccctg cgccgaggat tacctgagcg tcgtgctgaa tcagctgtgc     2820

gtgctgcacg agaaaacccc tgtgtccgac agagtgacca agtgctgtac cgagagcctg     2880

gtcaacagac ggccttgctt tagcgccctt gaggtggacg agacatacgt gcccaaagag     2940

ttcaacgccg agacattcac cttccacgcc gacatctgta ccctgagcga gaaagagcgg     3000

cagatcaaga aacagaccgc tctggtggaa ctggtcaagc acaagcccaa ggccaccaaa     3060

gaacaactga aggccgtgat ggacgacttc gccgcctttg tcgagaagtg ctgcaaggcc     3120

gatgacaaag agacatgctt cgccgaagag ggaaagaaac tggtggccgc ctctcaagcc     3180

gctctgggac tttaa                                                      3195


<210>  8
<211>  1757
<212>  DNA
<213>  Homo sapiens


<220>
<221>  Albumin
<222>  (1)..(1757)
<223>  albumin, codon optimized

<400>  8
gacgcccaca agagcgaggt ggcccacaga ttcaaggacc tgggagaaga gaacttcaag       60

gctctggtgc tgatcgcctt cgctcagtac ctccagcagt gcccattcga ggaccatgtg      120

aagctggtca acgaagtgac cgagttcgcc aagacctgcg tggccgatga gtccgccgag      180

aactgtgata agagcctgca caccctgttc ggcgacaagc tgtgtacagt ggccacactg      240

agagaaacct acggcgagat ggccgactgc tgcgccaaac aagagcccga gagaaacgag      300

tgcttcctcc agcacaagga cgataacccc aacctgccta gactcgtgcg gcccgaagtg      360

gatgtcatgt gcaccgcctt ccacgacaac gaggaaacct tcctgaagaa gtacctgtac      420

gagatcgcca gacggcaccc ctacttttac gcccctgagc tgctgttctt tgccaagaga      480

tacaaggccg ccttcaccga gtgttgccag gccgctgata aggccgcttg tctgctgcct      540

aagctggatg agctgcgcga cgagggcaaa gcctcttctg ccaagcagag actgaagtgc      600

gccagcctcc agaagtttgg cgagagagcc tttaaggcct gggccgtcgc tagactgagc      660

cagagatttc ccaaggccga gtttgccgag gtgtccaagc tggttaccga cctgaccaag      720

gtgcacacag agtgctgtca cggcgatctg ctggaatgcg ccgacgatag agccgatctg      780

gccaagtaca tctgcgagaa tcaggacagc atcagctcca agctgaaaga atgctgcgag      840

aagcccctgc tcgaaaagag ccactgtatc gctgaggtgg aaaacgacga gatgcccgcc      900

gatctgcctt ctctggccgc cgattttgtg gaaagcaagg acgtgtgcaa gaattacgcc      960

gaggccaagg atgtgttcct gggcatgttt ctgtatgagt acgcccgcag acaccccgac     1020

tactctgttg tgctgctgct gagactggcc aaaacctacg agactaccct ggaaaagtgc     1080

tgtgccgccg ctgatcctca cgagtgttac gccaaagtgt tcgacgagtt caagccactg     1140

gtggaagaac cccagaacct gatcaaacag aactgcgaac tgttcgagca gctgggcgag     1200

tacaagttcc agaacgccct gctcgtgcgg tacaccaaga aggtgcccca ggtttcaacc     1260

cctacactgg ttgaggtgtc ccggaacctg ggcaaagtgg gcagcaagtg ttgcaagcac     1320

cctgaggcca agagaatgcc ctgcgccgag gattacctga gcgtcgtgct gaatcagctg     1380

tgcgtgctgc acgagaaaac ccctgtgtcc gacagagtga ccaagtgctg taccgagagc     1440

ctggtcaaca gacggccttg ctttagcgcc cttgaggtgg acgagacata cgtgcccaaa     1500

gagttcaacg ccgagacatt caccttccac gccgacatct gtaccctgag cgagaaagag     1560

cggcagatca agaaacagac cgctctggtg gaactggtca agcacaagcc caaggccacc     1620

aaagaacaac tgaaggccgt gatggacgac ttcgccgcct ttgtcgagaa gtgctgcaag     1680

gccgatgaca aagagacatg cttcgccgaa gagggaaaga aactggtggc cgcctctcaa     1740

gccgctctgg gacttta                                                    1757


<210>  9
<211>  42
<212>  DNA
<213>  synthetic sequence


<220>
<221>  synthetic_linker
<222>  (1)..(42)
<223>  synthetic linker for replacement of B-domain in FVIII

<400>  9
agcttctccc agaatccacc agtcttaaaa agacaccaga ga                          42


<210>  10
<211>  14
<212>  PRT
<213>  synthetic protein sequence


<220>
<221>  synthetic_linker
<222>  (1)..(14)
<223>  synthetic linker to replace B-domain in FVIII

<400>  10

Ser Phe Ser Gln Asn Pro Pro Val Leu Lys Arg His Gln Arg 
1               5                   10                  


<210>  11
<211>  742
<212>  DNA
<213>  Homo sapiens


<220>
<221>  S/MAR_short
<222>  (1)..(742)
<223>  S/MAR_short derived from IFN_beta

<400>  11
aattgagatc taaataaact tataaattgt gagagaaatt aatgaatgtc taagttaatg       60

cagaaacgga gagacatact atattcatga actaaaagac ttaatattgt gaaggtatac      120

tttctttcca cataaatttg tagtcaatat gttcacccca aaaaagctgt ttgttaactt      180

gccaacctca ttctaaaatg tatatagaag cccaaaagac aataacaaaa atattcttgt      240

agaacaaaat gggaaagaat gttccactaa atatcaagat ttagagcaaa gcatgagatg      300

tgtggggata gacagtgagg ctgataaaat agagtagagc tcagaaacag acccattgat      360

atatgtaagt gacctatgaa aaaaatatgg cattttacaa tgggaaaatg atgatctttt      420

tcttttttag aaaaacaggg aaatatattt atatgtaaaa aataaaaggg aacccatatg      480

tcataccata cacacaaaaa aattccagtg aattataagt ctaaatggag aaggcaaaac      540

tttaaatctt ttagaaaata atatagaagc atgccatcat gacttcagtg tagagaaaaa      600

tttcttatga ctcaaagtcc taaccacaaa gaaaagattg ttaatttgaa tgatttagga      660

agtattccct ctgcttctgt cttctgaaag agattgtaga aagttgatac aatttttttt      720

tctttaaata tttgatagaa tt                                               742


<210>  12
<211>  1983
<212>  DNA
<213>  Homo sapiens


<220>
<221>  S/MAR_long
<222>  (1)..(1983)
<223>  S/MAR_long derived from IFN-beta

<400>  12
gatctaaata aacttataaa ttgtgagaga aattaatgaa tgtctaagtt aatgcagaaa       60

cggagagaca tactatattc atgaactaaa agacttaata ttgtgaaggt atactttctt      120

tccacataaa tttgtagtca atatgttcac cccaaaaaag ctgtttgtta acttgccaac      180

ctcattctaa aatgtatata gaagcccaaa agacaataac aaaaatattc ttgtagaaca      240

aaatgggaaa gaatgttcca ctaaatatca agatttagag caaagcatga gatgtgtggg      300

gatagacagt gaggctgata aaatagagta gagctcagaa acagacccat tgatatatgt      360

aagtgaccta tgaaaaaaat atggcatttt acaatgggaa aatgatgatc tttttctttt      420

ttagaaaaac agggaaatat atttatatgt aaaaaataaa agggaaccca tatgtcatac      480

catacacaca aaaaaattcc agtgaattat aagtctaaat ggagaaggca aaactttaaa      540

tcttttagaa aataatatag aagcatgcca tcatgacttc agtgtagaga aaaatttctt      600

atgactcaaa gtcctaacca caaagaaaag attgttaatt agattgcatg aatattaaga      660

cttattttta aaattaaaaa accattaaga aaagtcaggc catagaatga cagaaaatat      720

ttgcaacacc ccagtaaaga gaattgtaat atgcagatta taaaaagaag tcttacaaat      780

cagtaaaaaa taaaactaga caaaaatttg aacagatgaa agagaaactc taaataatca      840

ttacacatga gaaactcaat ctcagaaatc agagaactat cattgcatat acactaaatt      900

agagaaatat taaaaggcta agtaacatct gtggcaatat tgatggtata taaccttgat      960

atgatgtgat gagaacagta ctttacccca tgggcttcct ccccaaaccc ttaccccagt     1020

ataaatcatg acaaatatac tttaaaaacc attaccctat atctaaccag tactcctcaa     1080

aactgtcaag gtcatcaaaa ataagaaaag tctgaggaac tgtcaaaact aagaggaacc     1140

caaggagaca tgagaattat atgtaatgtg gcattctgaa tgagatccca gaacagaaaa     1200

agaacagtag ctaaaaaact aatgaaatat aaataaagtt tgaactttag ttttttttaa     1260

aaaagagtag cattaacacg gcaaagccat tttcatattt ttcttgaaca ttaagtacaa     1320

gtctataatt aaaaattttt taaatgtagt ctggaacatt gccagaaaca gaagtacaac     1380

agctatctgt gctgtcgcct aactatccat agctgattgg tctaaaatga gatacatcaa     1440

cgctcctcca tgttttttgt tttcttttta aatgaaaaac tttatttttt aagaggagtt     1500

tcaggttcat agcaaaattg agaggaaggt acattcaagc tgaggaagtt ttcctctatt     1560

cctagtttac tgagagattg catcatgaat gggtgttaaa ttttgtcaaa tgctttttct     1620

gtgtctatca atatgaccat gtgattttct tctttaacct gttgatggga caaattacgt     1680

taattgattt tcaaacgttg aaccaccctt acatatctgg aataaattct acttggttgt     1740

ggtgtatatt ttttgataca ttcttggatt ctttttgcta atattttgtt gaaaatgttt     1800

gtatctttgt tcatgagaga tattggtctg ttgttttctt ttcttgtaat gtcattttct     1860

agttccggta ttaaggtaat gctggcctag ttgaatgatt taggaagtat tccctctgct     1920

tctgtcttct gaaagagatt gtagaaagtt gatacaattt ttttttcttt aaatatttga     1980

tag                                                                   1983


<210>  13
<211>  56
<212>  DNA
<213>  Homo sapiens


<220>
<221>  CBX3
<222>  (1)..(56)
<223>  promoter CBX3, part of UCOE

<400>  13
cccgggaggt ggtccctgca gttacgccaa tgataacccc cgccagaaaa atctta           56


<210>  14
<211>  621
<212>  DNA
<213>  Homo sapiens


<220>
<221>  transcript_CBX3
<222>  (1)..(621)
<223>  transcript to CBX3, part of UCOE

<400>  14
tagccttccc tttttgtttt ccgtgcccca actcggcgga ttgactcggc cccttccgga       60

aacacccgaa tcaacttcta gtcaaattat tgttcacgcc gcaatgaccc acccctggcc      120

cgcgtctgtg gaactgaccc ctggtgaaca ggagagttcg ctgctgaaag tggtcccaaa      180

ggggtactag tttttaagct cccaactccc cctcccccag cgtctggagg attccacacc      240

ctcgcaccgc aggggcgagg aagtgggcgg agtccggttt tggcgccagc cgctgaggct      300

gccaagcaga aaagccaccg ctgaggagac tccggtcact gtcctcgccc cgcctccccc      360

ttccctcccc ttggggacca ccgggcgcca cgccgcgaac gctaagtgcc gcggtcgtcg      420

gcgcctccgc cctccccctt gggccccaat tcccagcggg cgcggcgcgc ggcccctccc      480

cccgccgggc gcgcgcccgc tgccccgccc ttcgtggccg cccggcgtgg gcggtgccac      540

ccctcccccc ggcggccccg cgcgcagctc ccggctccct cccccttcgg atgtggcttg      600

agctgtaggc gcggagggcc g                                                621


<210>  15
<211>  86
<212>  DNA
<213>  Homo sapiens


<220>
<221>  HNRNPA2_promoter
<222>  (1)..(86)
<223>  HNRNPA2 promoter, part of UCOE

<400>  15
gccgctagag ccgctgccgc cgcttttcta gaaccttccc ccccactaac gcgtcttccg       60

ctacgtcagg ccgtcgcgta aacgcc                                            86


<210>  16
<211>  165
<212>  DNA
<213>  Homo sapiens


<220>
<221>  transcript_HNRNPA2
<222>  (1)..(165)
<223>  transcript to HNRNPA2, part of UCOE

<400>  16
gactcagtcg cttcagcccg atttcccgca gccgagcgag atgagagaca tctccgcgga       60

cgaacacgaa ccggactcgt cctggcgctg tagtgagaac tgccgctgat cgagaaacaa      120

ctctgcgagg agcacctccg cacgggaccc ggcgctgctg ctact                      165


<210>  17
<211>  588
<212>  DNA
<213>  Cytomegalovirus


<220>
<221>  CMV Promoter
<222>  (1)..(588)
<223>  CMV Promoter

<400>  17
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagat                   588


<210>  18
<211>  458
<212>  DNA
<213>  Homo sapiens


<220>
<221>  EF1alpha_promoter_opt_splice_sites
<222>  (1)..(458)
<223>  promoter derived from human EF1alpha plus splice sites derived 
       from human EF1alpha

<400>  18
ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg       60

ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt      120

gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca      180

gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc      240

gtgtgtggtt cccgcgggcc tggcctcttt acgggttatc gcccttgcgt gccttgaatt      300

acttccacgc ccctggctgc agtacgtgat tcttgatccc gattctcctt ggaatttgcc      360

ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt      420

cttccatttc aggtgtcgag gcctgaattc gtcgactg                              458


<210>  19
<211>  4374
<212>  DNA
<213>  Homo sapiens


<220>
<221>  FVIII
<222>  (1)..(4374)
<223>  coagulation factor FVIII SQ with deleted B-domain, codon 
       optimized

<400>  19
atgcagatcg agctctctac ctgcttcttc ctgtgcctgc tgcggttctg cttcagcgcc       60

accagacggt actatctggg cgccgtggaa ctgagctggg actacatgca gagcgacctg      120

ggcgagctgc ccgtggatgc cagattccct ccaagagtgc ccaagagctt ccccttcaac      180

acctccgtgg tgtataagaa aaccctgttc gtggagttca ccgaccacct gttcaatatc      240

gccaagccca gacccccctg gatgggcctg ctgggaccta caattcaggc cgaggtgtac      300

gacaccgtcg tgatcaccct gaagaacatg gccagccacc ccgtgtctct gcatgccgtg      360

ggagtgtcct actggaaggc ctctgagggc gccgagtacg acgatcagac cagccagcgc      420

gagaaagagg acgacaaggt gttccctggc ggcagccaca cctacgtgtg gcaggtgctg      480

aaagaaaacg gccccatggc ctccgaccct ctgtgcctga catacagcta cctgagccac      540

gtggacctcg tgaaggacct gaacagcggc ctgatcggag ccctgctcgt gtgtagagag      600

ggcagcctgg ccaaagagaa aacccagacc ctgcacaagt tcatcctgct gttcgccgtg      660

ttcgacgagg gcaagagctg gcacagcgag acaaagaaca gcctgatgca ggaccgggac      720

gccgcctctg ctagagcctg gcccaaaatg cacaccgtga acggctacgt gaacagaagc      780

ctgcccggac tgatcggctg ccaccggaag tctgtgtact ggcacgtgat cggcatgggc      840

accacccctg aggtgcacag catctttctg gaaggacaca cctttctcgt gcggaaccac      900

cggcaggcca gcctggaaat cagccctatc accttcctga ccgcccagac actgctgatg      960

gacctgggcc agtttctgct gttctgccac atcagctccc accagcacga cggcatggaa     1020

gcctacgtga aggtggacag ctgccccgag gaaccccagc tgcggatgaa gaacaacgag     1080

gaagccgagg actacgacga cgacctgacc gacagcgaga tggacgtggt gcgcttcgac     1140

gacgataaca gccccagctt catccagatc agaagcgtgg ccaagaagca ccccaagacc     1200

tgggtgcact atatcgccgc cgaggaagag gactgggatt acgcccctct ggtgctggcc     1260

cccgacgaca gaagctacaa gagccagtac ctgaacaatg gcccccagcg gatcggccgg     1320

aagtacaaga aagtgcggtt catggcctac accgacgaga cattcaagac cagagaggcc     1380

atccagcacg agagcggcat cctgggccct ctgctgtatg gcgaagtggg cgacaccctg     1440

ctgatcatct tcaagaacca ggccagcaga ccctacaaca tctaccctca cggcatcacc     1500

gacgtgcggc ccctgtattc tcggagactg cccaagggcg tgaaacacct gaaggacttc     1560

cccatcctgc ccggcgagat cttcaagtac aagtggaccg tgaccgtgga agatggcccc     1620

accaagagcg accccagatg cctgacacgg tactacagca gcttcgtgaa catggaacgg     1680

gacctggcct ccggcctgat tggcccactg ctgatctgct acaaagaaag cgtggaccag     1740

cggggcaacc agatcatgag cgacaagcgg aacgtgatcc tgtttagcgt gttcgatgag     1800

aaccggtcct ggtatctgac cgagaatatc cagcggttcc tgcccaaccc tgccggcgtg     1860

cagctggaag atcctgagtt ccaggcctcc aacatcatgc actccatcaa tggctatgtg     1920

ttcgacagcc tgcagctgag cgtgtgcctg cacgaggtgg cctactggta catcctgagc     1980

atcggggccc agaccgactt cctgtccgtg ttcttctccg gctacacctt caagcacaag     2040

atggtgtacg aggataccct gaccctgttc ccctttagcg gcgaaaccgt gttcatgagc     2100

atggaaaacc ccggcctgtg gatcctgggc tgccacaaca gcgacttccg gaacagaggc     2160

atgaccgccc tgctgaaggt gtccagctgc gacaagaaca ccggcgacta ctacgaggac     2220

agctatgagg acatcagcgc ctacctgctg agcaagaaca atgccatcga gcccagaagc     2280

ttcagccaga acccccccgt gctgaagcgg caccagagag agatcacccg gaccaccctg     2340

cagtccgacc aggaagagat cgattacgac gacaccatca gcgtggaaat gaagaaagaa     2400

gatttcgaca tctacgacga ggacgagaac cagagccccc ggtcctttca gaaaaagacc     2460

cggcactact tcattgccgc tgtggaacgg ctgtgggact acggcatgag cagcagccct     2520

cacgtgctga gaaacagggc ccagagcggc agcgtgcccc agttcaaaaa ggtggtgttc     2580

caggagttta ccgacggcag cttcacccag cctctgtacc ggggagagct gaacgagcac     2640

ctgggactgc tgggccccta tatcagagcc gaagtggaag ataacatcat ggtcaccttc     2700

cggaatcagg cctcccggcc ctacagcttc tacagctccc tgatcagcta cgaagaggac     2760

cagagacagg gcgctgagcc ccggaagaac ttcgtgaagc ccaacgagac taagacctac     2820

ttttggaagg tgcagcacca catggcccct acaaaggacg agttcgactg caaggcctgg     2880

gcctacttct ccgatgtgga cctggaaaag gacgtgcact ctgggctgat cggccccctg     2940

ctcgtgtgcc acaccaacac cctgaatccc gcccacggca gacaagtgac agtgcaggaa     3000

tttgccctgt tcttcaccat cttcgacgaa acaaagagct ggtacttcac cgaaaacatg     3060

gaaagaaact gccgggctcc ctgcaacatc cagatggaag atcccacctt caaagagaac     3120

taccggttcc acgccatcaa cggctacatc atggacacac tgcccggcct cgtgatggct     3180

caggatcagc ggatccggtg gtatctgctg tccatgggct ccaacgagaa catccacagc     3240

atccacttca gcggccacgt gttcaccgtg cggaaaaaag aagagtacaa aatggccctg     3300

tataacctgt accccggcgt gttcgagaca gtggaaatgc tgcctagcaa ggccggcatc     3360

tggcgggtgg aatgtctgat cggcgagcat ctgcacgctg ggatgagcac actgtttctg     3420

gtgtactcca acaagtgcca gacacctctg ggcatggcct ctggccacat ccgggacttt     3480

cagatcacag ccagcggcca gtatggccag tgggccccaa aactggccag actgcactac     3540

agcggcagca tcaacgcctg gtccaccaaa gagcccttca gctggatcaa ggtggacctg     3600

ctggctccca tgatcatcca cggaatcaag acccagggcg ccagacagaa gttcagcagc     3660

ctgtatatca gccagttcat catcatgtac tccctggacg gcaagaagtg gcagacctac     3720

cggggcaata gcaccggcac cctgatggtg ttcttcggca acgtggactc cagcggcatt     3780

aagcacaaca tcttcaaccc ccccatcatt gcccggtaca tccggctgca ccccacccac     3840

tacagcatcc ggtccaccct gagaatggaa ctgatgggct gcgacctgaa ctcctgcagc     3900

atgcccctgg ggatggaaag caaggccatc tccgacgccc agatcaccgc ctccagctac     3960

ttcaccaaca tgttcgccac ctggtccccc agcaaggccc ggctgcatct gcagggcaga     4020

agcaatgctt ggaggcccca agtgaacaac cccaaagaat ggctgcaggt ggacttccag     4080

aaaaccatga aagtgaccgg cgtgaccacc cagggcgtga agtctctgct gacctctatg     4140

tacgtgaaag agttcctgat ctccagcagc caggacggcc accagtggac cctgtttttc     4200

cagaacggca aagtgaaagt gtttcagggg aaccaggact ccttcacccc cgtcgtgaat     4260

agcctggacc ctccactgct gaccagatac cttcgaatcc accctcagtc ttgggtgcac     4320

cagattgctc tgcggatgga agtgctggga tgcgaggccc aggacctgta ctga           4374


<210>  20
<211>  42
<212>  DNA
<213>  Synthetic sequence


<220>
<221>  synthetic_linker
<222>  (1)..(42)
<223>  synthetic linker for replacement of B-domain in FVIII, 
       codon optimized

<400>  20
agcttcagcc agaacccccc cgtgctgaag cggcaccaga ga                          42


<210>  21
<211>  271
<212>  DNA
<213>  Homo sapiens


<220>
<221>  UCOE_enhancer
<222>  (1)..(271)
<223>  enhancer region in a UCOE

<400>  21
ctatccgccg ccaatggcgg gaaggctcta cgccccacct tacgccaaat gcgtactcct       60

cccacccttg cggccagaga cagtacccga cgttacttcc gtaaatgcgc tcaatgaatt      120

gcggaaggct agagtcctgc tagttactac ctcttggaat agggtcccgc cccctgcctt      180

ggcgcaaggc aggtgagaaa cggtcgcgca gtttgaaatt aacgccgacg ggaggggctt      240

aatccgcagc ctggagatcc agccccctca a                                     271


<210>  22
<211>  1201
<212>  DNA
<213>  Homo sapiens


<220>
<221>  UCOE_redesigned
<222>  (1)..(1201)
<223>  UCOE with CBX3 promoter and transcript, HNRNPA2 promoter and 
       transcript in reverse orientation and enhancer region

<400>  22
gactcagtcg cttcagcccg atttcccgca gccgagcgag atgagagaca tctccgcgga       60

cgaacacgaa ccggactcgt cctggcgctg tagtgagaac tgccgctgat cgagaaacaa      120

ctctgcgagg agcacctccg cacgggaccc ggcgctgctg ctactgccgc tagagccgct      180

gccgccgctt ttctagaacc ttccccccca ctaacgcgtc ttccgctacg tcaggccgtc      240

gcgtaaacgc cctatccgcc gccaatggcg ggaaggctct acgccccacc ttacgccaaa      300

tgcgtactcc tcccaccctt gcggccagag acagtacccg acgttacttc cgtaaatgcg      360

ctcaatgaat tgcggaaggc tagagtcctg ctagttacta cctcttggaa tagggtcccg      420

ccccctgcct tggcgcaagg caggtgagaa acggtcgcgc agtttgaaat taacgccgac      480

gggaggggct taatccgcag cctggagatc cagccccctc aacccgggag gtggtccctg      540

cagttacgcc aatgataacc cccgccagaa aaatcttagt agccttccct ttttgttttc      600

cgtgccccaa ctcggcggat tgactcggcc ccttccggaa acacccgaat caacttctag      660

tcaaattatt gttcacgccg caatgaccca cccctggccc gcgtctgtgg aactgacccc      720

tggtgaacag gagagttcgc tgctgaaagt ggtcccaaag gggtactagt ttttaagctc      780

ccaactcccc ctcccccagc gtctggagga ttccacaccc tcgcaccgca ggggcgagga      840

agtgggcgga gtccggtttt ggcgccagcc gctgaggctg ccaagcagaa aagccaccgc      900

tgaggagact ccggtcactg tcctcgcccc gcctccccct tccctcccct tggggaccac      960

cgggcgccac gccgcgaacg ctaagtgccg cggtcgtcgg cgcctccgcc ctcccccttg     1020

ggccccaatt cccagcgggc gcggcgcgcg gcccctcccc ccgccgggcg cgcgcccgct     1080

gccccgccct tcgtggccgc ccggcgtggg cggtgccacc cctccccccg gcggccccgc     1140

gcgcagctcc cggctccctc ccccttcgga tgtggcttga gctgtaggcg cggagggccg     1200

t                                                                     1201


<210>  23
<211>  461
<212>  PRT
<213>  Homo sapiens


<220>
<221>  FIX
<222>  (1)..(461)
<223>  amino acid sequence factor FIX

<400>  23

Met Gln Arg Val Asn Met Ile Met Ala Glu Ser Pro Gly Leu Ile Thr 
1               5                   10                  15      


Ile Cys Leu Leu Gly Tyr Leu Leu Ser Ala Glu Cys Thr Val Phe Leu 
            20                  25                  30          


Asp His Glu Asn Ala Asn Lys Ile Leu Asn Arg Pro Lys Arg Tyr Asn 
        35                  40                  45              


Ser Gly Lys Leu Glu Glu Phe Val Gln Gly Asn Leu Glu Arg Glu Cys 
    50                  55                  60                  


Met Glu Glu Lys Cys Ser Phe Glu Glu Ala Arg Glu Val Phe Glu Asn 
65                  70                  75                  80  


Thr Glu Arg Thr Thr Glu Phe Trp Lys Gln Tyr Val Asp Gly Asp Gln 
                85                  90                  95      


Cys Glu Ser Asn Pro Cys Leu Asn Gly Gly Ser Cys Lys Asp Asp Ile 
            100                 105                 110         


Asn Ser Tyr Glu Cys Trp Cys Pro Phe Gly Phe Glu Gly Lys Asn Cys 
        115                 120                 125             


Glu Leu Asp Val Thr Cys Asn Ile Lys Asn Gly Arg Cys Glu Gln Phe 
    130                 135                 140                 


Cys Lys Asn Ser Ala Asp Asn Lys Val Val Cys Ser Cys Thr Glu Gly 
145                 150                 155                 160 


Tyr Arg Leu Ala Glu Asn Gln Lys Ser Cys Glu Pro Ala Val Pro Phe 
                165                 170                 175     


Pro Cys Gly Arg Val Ser Val Ser Gln Thr Ser Lys Leu Thr Arg Ala 
            180                 185                 190         


Glu Thr Val Phe Pro Asp Val Asp Tyr Val Asn Ser Thr Glu Ala Glu 
        195                 200                 205             


Thr Ile Leu Asp Asn Ile Thr Gln Ser Thr Gln Ser Phe Asn Asp Phe 
    210                 215                 220                 


Thr Arg Val Val Gly Gly Glu Asp Ala Lys Pro Gly Gln Phe Pro Trp 
225                 230                 235                 240 


Gln Val Val Leu Asn Gly Lys Val Asp Ala Phe Cys Gly Gly Ser Ile 
                245                 250                 255     


Val Asn Glu Lys Trp Ile Val Thr Ala Ala His Cys Val Glu Thr Gly 
            260                 265                 270         


Val Lys Ile Thr Val Val Ala Gly Glu His Asn Ile Glu Glu Thr Glu 
        275                 280                 285             


His Thr Glu Gln Lys Arg Asn Val Ile Arg Ile Ile Pro His His Asn 
    290                 295                 300                 


Tyr Asn Ala Ala Ile Asn Lys Tyr Asn His Asp Ile Ala Leu Leu Glu 
305                 310                 315                 320 


Leu Asp Glu Pro Leu Val Leu Asn Ser Tyr Val Thr Pro Ile Cys Ile 
                325                 330                 335     


Ala Asp Lys Glu Tyr Thr Asn Ile Phe Leu Lys Phe Gly Ser Gly Tyr 
            340                 345                 350         


Val Ser Gly Trp Gly Arg Val Phe His Lys Gly Arg Ser Ala Leu Val 
        355                 360                 365             


Leu Gln Tyr Leu Arg Val Pro Leu Val Asp Arg Ala Thr Cys Leu Arg 
    370                 375                 380                 


Ser Thr Lys Phe Thr Ile Tyr Asn Asn Met Phe Cys Ala Gly Phe His 
385                 390                 395                 400 


Glu Gly Gly Arg Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro His Val 
                405                 410                 415     


Thr Glu Val Glu Gly Thr Ser Phe Leu Thr Gly Ile Ile Ser Trp Gly 
            420                 425                 430         


Glu Glu Cys Ala Met Lys Gly Lys Tyr Gly Ile Tyr Thr Lys Val Ser 
        435                 440                 445             


Arg Tyr Val Asn Trp Ile Lys Glu Lys Thr Lys Leu Thr 
    450                 455                 460     


<210>  24
<211>  460
<212>  PRT
<213>  Homo sapiens


<220>
<221>  FIX_padua
<222>  (1)..(460)
<223>  amino acid sequence factor FIX padua

<400>  24

Met Gln Arg Val Asn Met Ile Met Ala Glu Ser Pro Gly Leu Ile Thr 
1               5                   10                  15      


Ile Cys Leu Leu Gly Tyr Leu Leu Ser Ala Glu Cys Thr Val Phe Leu 
            20                  25                  30          


Asp His Glu Asn Ala Asn Lys Ile Leu Asn Arg Pro Lys Arg Tyr Asn 
        35                  40                  45              


Ser Gly Lys Leu Glu Glu Phe Val Gln Gly Asn Leu Glu Arg Glu Cys 
    50                  55                  60                  


Met Glu Glu Lys Cys Ser Phe Glu Glu Ala Arg Glu Val Phe Glu Asn 
65                  70                  75                  80  


Thr Glu Arg Thr Thr Glu Phe Trp Lys Gln Tyr Val Asp Gly Asp Gln 
                85                  90                  95      


Cys Glu Ser Asn Pro Cys Leu Asn Gly Gly Ser Cys Lys Asp Asp Ile 
            100                 105                 110         


Asn Ser Tyr Glu Cys Trp Cys Pro Phe Gly Phe Glu Gly Lys Asn Cys 
        115                 120                 125             


Glu Leu Asp Val Thr Cys Asn Ile Lys Asn Gly Arg Cys Glu Gln Phe 
    130                 135                 140                 


Cys Lys Asn Ser Ala Asp Asn Lys Val Val Cys Ser Cys Thr Glu Gly 
145                 150                 155                 160 


Tyr Arg Leu Ala Glu Asn Gln Lys Ser Cys Glu Pro Ala Val Pro Phe 
                165                 170                 175     


Pro Cys Gly Arg Val Ser Val Ser Gln Thr Ser Lys Leu Thr Arg Ala 
            180                 185                 190         


Glu Thr Val Phe Pro Asp Val Asp Tyr Val Asn Ser Thr Glu Ala Glu 
        195                 200                 205             


Thr Ile Leu Asp Asn Ile Thr Gln Ser Thr Gln Ser Phe Asn Asp Phe 
    210                 215                 220                 


Thr Arg Val Val Gly Gly Glu Asp Ala Lys Pro Gly Gln Phe Pro Trp 
225                 230                 235                 240 


Gln Val Val Leu Asn Gly Lys Val Asp Ala Phe Cys Gly Gly Ser Ile 
                245                 250                 255     


Val Asn Glu Lys Trp Ile Val Thr Ala Ala His Cys Val Glu Thr Gly 
            260                 265                 270         


Val Lys Ile Thr Val Val Ala Gly Glu His Asn Ile Glu Glu Thr Glu 
        275                 280                 285             


His Thr Glu Gln Lys Arg Asn Val Ile Arg Ile Ile Pro His His Asn 
    290                 295                 300                 


Tyr Asn Ala Ala Ile Asn Lys Tyr Asn His Asp Ile Ala Leu Leu Glu 
305                 310                 315                 320 


Leu Asp Glu Pro Leu Val Leu Asn Ser Tyr Val Thr Pro Ile Cys Ile 
                325                 330                 335     


Ala Asp Lys Glu Tyr Thr Asn Ile Phe Leu Lys Phe Gly Ser Gly Tyr 
            340                 345                 350         


Val Ser Gly Trp Gly Arg Val Phe His Lys Gly Arg Ser Ala Leu Val 
        355                 360                 365             


Leu Gln Tyr Leu Arg Val Pro Leu Val Asp Arg Ala Thr Cys Leu Leu 
    370                 375                 380                 


Ser Thr Lys Phe Thr Ile Tyr Asn Asn Met Phe Cys Ala Gly Phe His 
385                 390                 395                 400 


Glu Gly Gly Arg Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro His Val 
                405                 410                 415     


Thr Glu Val Glu Gly Thr Ser Phe Leu Thr Gly Ile Ile Ser Trp Gly 
            420                 425                 430         


Glu Glu Cys Ala Met Lys Gly Lys Tyr Gly Ile Tyr Thr Lys Val Ser 
        435                 440                 445             


Arg Tyr Val Asn Trp Ile Lys Glu Lys Thr Lys Leu 
    450                 455                 460 


<210>  25
<211>  1064
<212>  PRT
<213>  Homo sapiens


<220>
<221>  FIX_albumin_fusion
<222>  (1)..(1064)
<223>  amino acid sequence FIX_albumin fusion

<400>  25

Met Gln Arg Val Asn Met Ile Met Ala Glu Ser Pro Gly Leu Ile Thr 
1               5                   10                  15      


Ile Cys Leu Leu Gly Tyr Leu Leu Ser Ala Glu Cys Thr Val Phe Leu 
            20                  25                  30          


Asp His Glu Asn Ala Asn Lys Ile Leu Asn Arg Pro Lys Arg Tyr Asn 
        35                  40                  45              


Ser Gly Lys Leu Glu Glu Phe Val Gln Gly Asn Leu Glu Arg Glu Cys 
    50                  55                  60                  


Met Glu Glu Lys Cys Ser Phe Glu Glu Ala Arg Glu Val Phe Glu Asn 
65                  70                  75                  80  


Thr Glu Arg Thr Thr Glu Phe Trp Lys Gln Tyr Val Asp Gly Asp Gln 
                85                  90                  95      


Cys Glu Ser Asn Pro Cys Leu Asn Gly Gly Ser Cys Lys Asp Asp Ile 
            100                 105                 110         


Asn Ser Tyr Glu Cys Trp Cys Pro Phe Gly Phe Glu Gly Lys Asn Cys 
        115                 120                 125             


Glu Leu Asp Val Thr Cys Asn Ile Lys Asn Gly Arg Cys Glu Gln Phe 
    130                 135                 140                 


Cys Lys Asn Ser Ala Asp Asn Lys Val Val Cys Ser Cys Thr Glu Gly 
145                 150                 155                 160 


Tyr Arg Leu Ala Glu Asn Gln Lys Ser Cys Glu Pro Ala Val Pro Phe 
                165                 170                 175     


Pro Cys Gly Arg Val Ser Val Ser Gln Thr Ser Lys Leu Thr Arg Ala 
            180                 185                 190         


Glu Thr Val Phe Pro Asp Val Asp Tyr Val Asn Ser Thr Glu Ala Glu 
        195                 200                 205             


Thr Ile Leu Asp Asn Ile Thr Gln Ser Thr Gln Ser Phe Asn Asp Phe 
    210                 215                 220                 


Thr Arg Val Val Gly Gly Glu Asp Ala Lys Pro Gly Gln Phe Pro Trp 
225                 230                 235                 240 


Gln Val Val Leu Asn Gly Lys Val Asp Ala Phe Cys Gly Gly Ser Ile 
                245                 250                 255     


Val Asn Glu Lys Trp Ile Val Thr Ala Ala His Cys Val Glu Thr Gly 
            260                 265                 270         


Val Lys Ile Thr Val Val Ala Gly Glu His Asn Ile Glu Glu Thr Glu 
        275                 280                 285             


His Thr Glu Gln Lys Arg Asn Val Ile Arg Ile Ile Pro His His Asn 
    290                 295                 300                 


Tyr Asn Ala Ala Ile Asn Lys Tyr Asn His Asp Ile Ala Leu Leu Glu 
305                 310                 315                 320 


Leu Asp Glu Pro Leu Val Leu Asn Ser Tyr Val Thr Pro Ile Cys Ile 
                325                 330                 335     


Ala Asp Lys Glu Tyr Thr Asn Ile Phe Leu Lys Phe Gly Ser Gly Tyr 
            340                 345                 350         


Val Ser Gly Trp Gly Arg Val Phe His Lys Gly Arg Ser Ala Leu Val 
        355                 360                 365             


Leu Gln Tyr Leu Arg Val Pro Leu Val Asp Arg Ala Thr Cys Leu Arg 
    370                 375                 380                 


Ser Thr Lys Phe Thr Ile Tyr Asn Asn Met Phe Cys Ala Gly Phe His 
385                 390                 395                 400 


Glu Gly Gly Arg Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro His Val 
                405                 410                 415     


Thr Glu Val Glu Gly Thr Ser Phe Leu Thr Gly Ile Ile Ser Trp Gly 
            420                 425                 430         


Glu Glu Cys Ala Met Lys Gly Lys Tyr Gly Ile Tyr Thr Lys Val Ser 
        435                 440                 445             


Arg Tyr Val Asn Trp Ile Lys Glu Lys Thr Lys Leu Thr Pro Val Ser 
    450                 455                 460                 


Gln Thr Ser Lys Leu Thr Arg Ala Glu Thr Val Phe Pro Asp Val Asp 
465                 470                 475                 480 


Ala His Lys Ser Glu Val Ala His Arg Phe Lys Asp Leu Gly Glu Glu 
                485                 490                 495     


Asn Phe Lys Ala Leu Val Leu Ile Ala Phe Ala Gln Tyr Leu Gln Gln 
            500                 505                 510         


Cys Pro Phe Glu Asp His Val Lys Leu Val Asn Glu Val Thr Glu Phe 
        515                 520                 525             


Ala Lys Thr Cys Val Ala Asp Glu Ser Ala Glu Asn Cys Asp Lys Ser 
    530                 535                 540                 


Leu His Thr Leu Phe Gly Asp Lys Leu Cys Thr Val Ala Thr Leu Arg 
545                 550                 555                 560 


Glu Thr Tyr Gly Glu Met Ala Asp Cys Cys Ala Lys Gln Glu Pro Glu 
                565                 570                 575     


Arg Asn Glu Cys Phe Leu Gln His Lys Asp Asp Asn Pro Asn Leu Pro 
            580                 585                 590         


Arg Leu Val Arg Pro Glu Val Asp Val Met Cys Thr Ala Phe His Asp 
        595                 600                 605             


Asn Glu Glu Thr Phe Leu Lys Lys Tyr Leu Tyr Glu Ile Ala Arg Arg 
    610                 615                 620                 


His Pro Tyr Phe Tyr Ala Pro Glu Leu Leu Phe Phe Ala Lys Arg Tyr 
625                 630                 635                 640 


Lys Ala Ala Phe Thr Glu Cys Cys Gln Ala Ala Asp Lys Ala Ala Cys 
                645                 650                 655     


Leu Leu Pro Lys Leu Asp Glu Leu Arg Asp Glu Gly Lys Ala Ser Ser 
            660                 665                 670         


Ala Lys Gln Arg Leu Lys Cys Ala Ser Leu Gln Lys Phe Gly Glu Arg 
        675                 680                 685             


Ala Phe Lys Ala Trp Ala Val Ala Arg Leu Ser Gln Arg Phe Pro Lys 
    690                 695                 700                 


Ala Glu Phe Ala Glu Val Ser Lys Leu Val Thr Asp Leu Thr Lys Val 
705                 710                 715                 720 


His Thr Glu Cys Cys His Gly Asp Leu Leu Glu Cys Ala Asp Asp Arg 
                725                 730                 735     


Ala Asp Leu Ala Lys Tyr Ile Cys Glu Asn Gln Asp Ser Ile Ser Ser 
            740                 745                 750         


Lys Leu Lys Glu Cys Cys Glu Lys Pro Leu Leu Glu Lys Ser His Cys 
        755                 760                 765             


Ile Ala Glu Val Glu Asn Asp Glu Met Pro Ala Asp Leu Pro Ser Leu 
    770                 775                 780                 


Ala Ala Asp Phe Val Glu Ser Lys Asp Val Cys Lys Asn Tyr Ala Glu 
785                 790                 795                 800 


Ala Lys Asp Val Phe Leu Gly Met Phe Leu Tyr Glu Tyr Ala Arg Arg 
                805                 810                 815     


His Pro Asp Tyr Ser Val Val Leu Leu Leu Arg Leu Ala Lys Thr Tyr 
            820                 825                 830         


Glu Thr Thr Leu Glu Lys Cys Cys Ala Ala Ala Asp Pro His Glu Cys 
        835                 840                 845             


Tyr Ala Lys Val Phe Asp Glu Phe Lys Pro Leu Val Glu Glu Pro Gln 
    850                 855                 860                 


Asn Leu Ile Lys Gln Asn Cys Glu Leu Phe Glu Gln Leu Gly Glu Tyr 
865                 870                 875                 880 


Lys Phe Gln Asn Ala Leu Leu Val Arg Tyr Thr Lys Lys Val Pro Gln 
                885                 890                 895     


Val Ser Thr Pro Thr Leu Val Glu Val Ser Arg Asn Leu Gly Lys Val 
            900                 905                 910         


Gly Ser Lys Cys Cys Lys His Pro Glu Ala Lys Arg Met Pro Cys Ala 
        915                 920                 925             


Glu Asp Tyr Leu Ser Val Val Leu Asn Gln Leu Cys Val Leu His Glu 
    930                 935                 940                 


Lys Thr Pro Val Ser Asp Arg Val Thr Lys Cys Cys Thr Glu Ser Leu 
945                 950                 955                 960 


Val Asn Arg Arg Pro Cys Phe Ser Ala Leu Glu Val Asp Glu Thr Tyr 
                965                 970                 975     


Val Pro Lys Glu Phe Asn Ala Glu Thr Phe Thr Phe His Ala Asp Ile 
            980                 985                 990         


Cys Thr Leu Ser Glu Lys Glu Arg  Gln Ile Lys Lys Gln  Thr Ala Leu 
        995                 1000                 1005             


Val Glu  Leu Val Lys His Lys  Pro Lys Ala Thr Lys  Glu Gln Leu 
    1010                 1015                 1020             


Lys Ala  Val Met Asp Asp Phe  Ala Ala Phe Val Glu  Lys Cys Cys 
    1025                 1030                 1035             


Lys Ala  Asp Asp Lys Glu Thr  Cys Phe Ala Glu Glu  Gly Lys Lys 
    1040                 1045                 1050             


Leu Val  Ala Ala Ser Gln Ala  Ala Leu Gly Leu 
    1055                 1060                 


<210>  26
<211>  1064
<212>  PRT
<213>  Homo sapiens


<220>
<221>  FIX_padua_albumin
<222>  (1)..(1064)
<223>  amino acid sequence FIX_padua albumin fusion

<400>  26

Met Gln Arg Val Asn Met Ile Met Ala Glu Ser Pro Gly Leu Ile Thr 
1               5                   10                  15      


Ile Cys Leu Leu Gly Tyr Leu Leu Ser Ala Glu Cys Thr Val Phe Leu 
            20                  25                  30          


Asp His Glu Asn Ala Asn Lys Ile Leu Asn Arg Pro Lys Arg Tyr Asn 
        35                  40                  45              


Ser Gly Lys Leu Glu Glu Phe Val Gln Gly Asn Leu Glu Arg Glu Cys 
    50                  55                  60                  


Met Glu Glu Lys Cys Ser Phe Glu Glu Ala Arg Glu Val Phe Glu Asn 
65                  70                  75                  80  


Thr Glu Arg Thr Thr Glu Phe Trp Lys Gln Tyr Val Asp Gly Asp Gln 
                85                  90                  95      


Cys Glu Ser Asn Pro Cys Leu Asn Gly Gly Ser Cys Lys Asp Asp Ile 
            100                 105                 110         


Asn Ser Tyr Glu Cys Trp Cys Pro Phe Gly Phe Glu Gly Lys Asn Cys 
        115                 120                 125             


Glu Leu Asp Val Thr Cys Asn Ile Lys Asn Gly Arg Cys Glu Gln Phe 
    130                 135                 140                 


Cys Lys Asn Ser Ala Asp Asn Lys Val Val Cys Ser Cys Thr Glu Gly 
145                 150                 155                 160 


Tyr Arg Leu Ala Glu Asn Gln Lys Ser Cys Glu Pro Ala Val Pro Phe 
                165                 170                 175     


Pro Cys Gly Arg Val Ser Val Ser Gln Thr Ser Lys Leu Thr Arg Ala 
            180                 185                 190         


Glu Thr Val Phe Pro Asp Val Asp Tyr Val Asn Ser Thr Glu Ala Glu 
        195                 200                 205             


Thr Ile Leu Asp Asn Ile Thr Gln Ser Thr Gln Ser Phe Asn Asp Phe 
    210                 215                 220                 


Thr Arg Val Val Gly Gly Glu Asp Ala Lys Pro Gly Gln Phe Pro Trp 
225                 230                 235                 240 


Gln Val Val Leu Asn Gly Lys Val Asp Ala Phe Cys Gly Gly Ser Ile 
                245                 250                 255     


Val Asn Glu Lys Trp Ile Val Thr Ala Ala His Cys Val Glu Thr Gly 
            260                 265                 270         


Val Lys Ile Thr Val Val Ala Gly Glu His Asn Ile Glu Glu Thr Glu 
        275                 280                 285             


His Thr Glu Gln Lys Arg Asn Val Ile Arg Ile Ile Pro His His Asn 
    290                 295                 300                 


Tyr Asn Ala Ala Ile Asn Lys Tyr Asn His Asp Ile Ala Leu Leu Glu 
305                 310                 315                 320 


Leu Asp Glu Pro Leu Val Leu Asn Ser Tyr Val Thr Pro Ile Cys Ile 
                325                 330                 335     


Ala Asp Lys Glu Tyr Thr Asn Ile Phe Leu Lys Phe Gly Ser Gly Tyr 
            340                 345                 350         


Val Ser Gly Trp Gly Arg Val Phe His Lys Gly Arg Ser Ala Leu Val 
        355                 360                 365             


Leu Gln Tyr Leu Arg Val Pro Leu Val Asp Arg Ala Thr Cys Leu Leu 
    370                 375                 380                 


Ser Thr Lys Phe Thr Ile Tyr Asn Asn Met Phe Cys Ala Gly Phe His 
385                 390                 395                 400 


Glu Gly Gly Arg Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro His Val 
                405                 410                 415     


Thr Glu Val Glu Gly Thr Ser Phe Leu Thr Gly Ile Ile Ser Trp Gly 
            420                 425                 430         


Glu Glu Cys Ala Met Lys Gly Lys Tyr Gly Ile Tyr Thr Lys Val Ser 
        435                 440                 445             


Arg Tyr Val Asn Trp Ile Lys Glu Lys Thr Lys Leu Thr Pro Val Ser 
    450                 455                 460                 


Gln Thr Ser Lys Leu Thr Arg Ala Glu Thr Val Phe Pro Asp Val Asp 
465                 470                 475                 480 


Ala His Lys Ser Glu Val Ala His Arg Phe Lys Asp Leu Gly Glu Glu 
                485                 490                 495     


Asn Phe Lys Ala Leu Val Leu Ile Ala Phe Ala Gln Tyr Leu Gln Gln 
            500                 505                 510         


Cys Pro Phe Glu Asp His Val Lys Leu Val Asn Glu Val Thr Glu Phe 
        515                 520                 525             


Ala Lys Thr Cys Val Ala Asp Glu Ser Ala Glu Asn Cys Asp Lys Ser 
    530                 535                 540                 


Leu His Thr Leu Phe Gly Asp Lys Leu Cys Thr Val Ala Thr Leu Arg 
545                 550                 555                 560 


Glu Thr Tyr Gly Glu Met Ala Asp Cys Cys Ala Lys Gln Glu Pro Glu 
                565                 570                 575     


Arg Asn Glu Cys Phe Leu Gln His Lys Asp Asp Asn Pro Asn Leu Pro 
            580                 585                 590         


Arg Leu Val Arg Pro Glu Val Asp Val Met Cys Thr Ala Phe His Asp 
        595                 600                 605             


Asn Glu Glu Thr Phe Leu Lys Lys Tyr Leu Tyr Glu Ile Ala Arg Arg 
    610                 615                 620                 


His Pro Tyr Phe Tyr Ala Pro Glu Leu Leu Phe Phe Ala Lys Arg Tyr 
625                 630                 635                 640 


Lys Ala Ala Phe Thr Glu Cys Cys Gln Ala Ala Asp Lys Ala Ala Cys 
                645                 650                 655     


Leu Leu Pro Lys Leu Asp Glu Leu Arg Asp Glu Gly Lys Ala Ser Ser 
            660                 665                 670         


Ala Lys Gln Arg Leu Lys Cys Ala Ser Leu Gln Lys Phe Gly Glu Arg 
        675                 680                 685             


Ala Phe Lys Ala Trp Ala Val Ala Arg Leu Ser Gln Arg Phe Pro Lys 
    690                 695                 700                 


Ala Glu Phe Ala Glu Val Ser Lys Leu Val Thr Asp Leu Thr Lys Val 
705                 710                 715                 720 


His Thr Glu Cys Cys His Gly Asp Leu Leu Glu Cys Ala Asp Asp Arg 
                725                 730                 735     


Ala Asp Leu Ala Lys Tyr Ile Cys Glu Asn Gln Asp Ser Ile Ser Ser 
            740                 745                 750         


Lys Leu Lys Glu Cys Cys Glu Lys Pro Leu Leu Glu Lys Ser His Cys 
        755                 760                 765             


Ile Ala Glu Val Glu Asn Asp Glu Met Pro Ala Asp Leu Pro Ser Leu 
    770                 775                 780                 


Ala Ala Asp Phe Val Glu Ser Lys Asp Val Cys Lys Asn Tyr Ala Glu 
785                 790                 795                 800 


Ala Lys Asp Val Phe Leu Gly Met Phe Leu Tyr Glu Tyr Ala Arg Arg 
                805                 810                 815     


His Pro Asp Tyr Ser Val Val Leu Leu Leu Arg Leu Ala Lys Thr Tyr 
            820                 825                 830         


Glu Thr Thr Leu Glu Lys Cys Cys Ala Ala Ala Asp Pro His Glu Cys 
        835                 840                 845             


Tyr Ala Lys Val Phe Asp Glu Phe Lys Pro Leu Val Glu Glu Pro Gln 
    850                 855                 860                 


Asn Leu Ile Lys Gln Asn Cys Glu Leu Phe Glu Gln Leu Gly Glu Tyr 
865                 870                 875                 880 


Lys Phe Gln Asn Ala Leu Leu Val Arg Tyr Thr Lys Lys Val Pro Gln 
                885                 890                 895     


Val Ser Thr Pro Thr Leu Val Glu Val Ser Arg Asn Leu Gly Lys Val 
            900                 905                 910         


Gly Ser Lys Cys Cys Lys His Pro Glu Ala Lys Arg Met Pro Cys Ala 
        915                 920                 925             


Glu Asp Tyr Leu Ser Val Val Leu Asn Gln Leu Cys Val Leu His Glu 
    930                 935                 940                 


Lys Thr Pro Val Ser Asp Arg Val Thr Lys Cys Cys Thr Glu Ser Leu 
945                 950                 955                 960 


Val Asn Arg Arg Pro Cys Phe Ser Ala Leu Glu Val Asp Glu Thr Tyr 
                965                 970                 975     


Val Pro Lys Glu Phe Asn Ala Glu Thr Phe Thr Phe His Ala Asp Ile 
            980                 985                 990         


Cys Thr Leu Ser Glu Lys Glu Arg  Gln Ile Lys Lys Gln  Thr Ala Leu 
        995                 1000                 1005             


Val Glu  Leu Val Lys His Lys  Pro Lys Ala Thr Lys  Glu Gln Leu 
    1010                 1015                 1020             


Lys Ala  Val Met Asp Asp Phe  Ala Ala Phe Val Glu  Lys Cys Cys 
    1025                 1030                 1035             


Lys Ala  Asp Asp Lys Glu Thr  Cys Phe Ala Glu Glu  Gly Lys Lys 
    1040                 1045                 1050             


Leu Val  Ala Ala Ser Gln Ala  Ala Leu Gly Leu 
    1055                 1060                 


<210>  27
<211>  585
<212>  PRT
<213>  Homo sapiens


<220>
<221>  mature_albumin
<222>  (1)..(585)
<223>  amino acid sequence mature albumin

<400>  27

Asp Ala His Lys Ser Glu Val Ala His Arg Phe Lys Asp Leu Gly Glu 
1               5                   10                  15      


Glu Asn Phe Lys Ala Leu Val Leu Ile Ala Phe Ala Gln Tyr Leu Gln 
            20                  25                  30          


Gln Cys Pro Phe Glu Asp His Val Lys Leu Val Asn Glu Val Thr Glu 
        35                  40                  45              


Phe Ala Lys Thr Cys Val Ala Asp Glu Ser Ala Glu Asn Cys Asp Lys 
    50                  55                  60                  


Ser Leu His Thr Leu Phe Gly Asp Lys Leu Cys Thr Val Ala Thr Leu 
65                  70                  75                  80  


Arg Glu Thr Tyr Gly Glu Met Ala Asp Cys Cys Ala Lys Gln Glu Pro 
                85                  90                  95      


Glu Arg Asn Glu Cys Phe Leu Gln His Lys Asp Asp Asn Pro Asn Leu 
            100                 105                 110         


Pro Arg Leu Val Arg Pro Glu Val Asp Val Met Cys Thr Ala Phe His 
        115                 120                 125             


Asp Asn Glu Glu Thr Phe Leu Lys Lys Tyr Leu Tyr Glu Ile Ala Arg 
    130                 135                 140                 


Arg His Pro Tyr Phe Tyr Ala Pro Glu Leu Leu Phe Phe Ala Lys Arg 
145                 150                 155                 160 


Tyr Lys Ala Ala Phe Thr Glu Cys Cys Gln Ala Ala Asp Lys Ala Ala 
                165                 170                 175     


Cys Leu Leu Pro Lys Leu Asp Glu Leu Arg Asp Glu Gly Lys Ala Ser 
            180                 185                 190         


Ser Ala Lys Gln Arg Leu Lys Cys Ala Ser Leu Gln Lys Phe Gly Glu 
        195                 200                 205             


Arg Ala Phe Lys Ala Trp Ala Val Ala Arg Leu Ser Gln Arg Phe Pro 
    210                 215                 220                 


Lys Ala Glu Phe Ala Glu Val Ser Lys Leu Val Thr Asp Leu Thr Lys 
225                 230                 235                 240 


Val His Thr Glu Cys Cys His Gly Asp Leu Leu Glu Cys Ala Asp Asp 
                245                 250                 255     


Arg Ala Asp Leu Ala Lys Tyr Ile Cys Glu Asn Gln Asp Ser Ile Ser 
            260                 265                 270         


Ser Lys Leu Lys Glu Cys Cys Glu Lys Pro Leu Leu Glu Lys Ser His 
        275                 280                 285             


Cys Ile Ala Glu Val Glu Asn Asp Glu Met Pro Ala Asp Leu Pro Ser 
    290                 295                 300                 


Leu Ala Ala Asp Phe Val Glu Ser Lys Asp Val Cys Lys Asn Tyr Ala 
305                 310                 315                 320 


Glu Ala Lys Asp Val Phe Leu Gly Met Phe Leu Tyr Glu Tyr Ala Arg 
                325                 330                 335     


Arg His Pro Asp Tyr Ser Val Val Leu Leu Leu Arg Leu Ala Lys Thr 
            340                 345                 350         


Tyr Glu Thr Thr Leu Glu Lys Cys Cys Ala Ala Ala Asp Pro His Glu 
        355                 360                 365             


Cys Tyr Ala Lys Val Phe Asp Glu Phe Lys Pro Leu Val Glu Glu Pro 
    370                 375                 380                 


Gln Asn Leu Ile Lys Gln Asn Cys Glu Leu Phe Glu Gln Leu Gly Glu 
385                 390                 395                 400 


Tyr Lys Phe Gln Asn Ala Leu Leu Val Arg Tyr Thr Lys Lys Val Pro 
                405                 410                 415     


Gln Val Ser Thr Pro Thr Leu Val Glu Val Ser Arg Asn Leu Gly Lys 
            420                 425                 430         


Val Gly Ser Lys Cys Cys Lys His Pro Glu Ala Lys Arg Met Pro Cys 
        435                 440                 445             


Ala Glu Asp Tyr Leu Ser Val Val Leu Asn Gln Leu Cys Val Leu His 
    450                 455                 460                 


Glu Lys Thr Pro Val Ser Asp Arg Val Thr Lys Cys Cys Thr Glu Ser 
465                 470                 475                 480 


Leu Val Asn Arg Arg Pro Cys Phe Ser Ala Leu Glu Val Asp Glu Thr 
                485                 490                 495     


Tyr Val Pro Lys Glu Phe Asn Ala Glu Thr Phe Thr Phe His Ala Asp 
            500                 505                 510         


Ile Cys Thr Leu Ser Glu Lys Glu Arg Gln Ile Lys Lys Gln Thr Ala 
        515                 520                 525             


Leu Val Glu Leu Val Lys His Lys Pro Lys Ala Thr Lys Glu Gln Leu 
    530                 535                 540                 


Lys Ala Val Met Asp Asp Phe Ala Ala Phe Val Glu Lys Cys Cys Lys 
545                 550                 555                 560 


Ala Asp Asp Lys Glu Thr Cys Phe Ala Glu Glu Gly Lys Lys Leu Val 
                565                 570                 575     


Ala Ala Ser Gln Ala Ala Leu Gly Leu 
            580                 585 


<210>  28
<211>  2802
<212>  DNA
<213>  Homo sapiens


<220>
<221>  FIX_wt
<222>  (1)..(2802)
<223>  factor FIX, wt sequence

<400>  28
accactttca caatctgcta gcaaaggtta tgcagcgcgt gaacatgatc atggcagaat       60

caccaggcct catcaccatc tgccttttag gatatctact cagtgctgaa tgtacagttt      120

ttcttgatca tgaaaacgcc aacaaaattc tgaatcggcc aaagaggtat aattcaggta      180

aattggaaga gtttgttcaa gggaaccttg agagagaatg tatggaagaa aagtgtagtt      240

ttgaagaagc acgagaagtt tttgaaaaca ctgaaagaac aactgaattt tggaagcagt      300

atgttgatgg agatcagtgt gagtccaatc catgtttaaa tggcggcagt tgcaaggatg      360

acattaattc ctatgaatgt tggtgtccct ttggatttga aggaaagaac tgtgaattag      420

atgtaacatg taacattaag aatggcagat gcgagcagtt ttgtaaaaat agtgctgata      480

acaaggtggt ttgctcctgt actgagggat atcgacttgc agaaaaccag aagtcctgtg      540

aaccagcagt gccatttcca tgtggaagag tttctgtttc acaaacttct aagctcaccc      600

gtgctgagac tgtttttcct gatgtggact atgtaaattc tactgaagct gaaaccattt      660

tggataacat cactcaaagc acccaatcat ttaatgactt cactcgggtt gttggtggag      720

aagatgccaa accaggtcaa ttcccttggc aggttgtttt gaatggtaaa gttgatgcat      780

tctgtggagg ctctatcgtt aatgaaaaat ggattgtaac tgctgcccac tgtgttgaaa      840

ctggtgttaa aattacagtt gtcgcaggtg aacataatat tgaggagaca gaacatacag      900

agcaaaagcg aaatgtgatt cgaattattc ctcaccacaa ctacaatgca gctattaata      960

agtacaacca tgacattgcc cttctggaac tggacgaacc cttagtgcta aacagctacg     1020

ttacacctat ttgcattgct gacaaggaat acacgaacat cttcctcaaa tttggatctg     1080

gctatgtaag tggctgggga agagtcttcc acaaagggag atcagcttta gttcttcagt     1140

accttagagt tccacttgtt gaccgagcca catgtcttcg atctacaaag ttcaccatct     1200

ataacaacat gttctgtgct ggcttccatg aaggaggtag agattcatgt caaggagata     1260

gtgggggacc ccatgttact gaagtggaag ggaccagttt cttaactgga attattagct     1320

ggggtgaaga gtgtgcaatg aaaggcaaat atggaatata taccaaggta tcccggtatg     1380

tcaactggat taaggaaaaa acaaagctca cttaatgaaa gatggatttc caaggttaat     1440

tcattggaat tgaaaattaa cagggcctct cactaactaa tcactttccc atcttttgtt     1500

agatttgaat atatacattc tatgatcatt gctttttctc tttacagggg agaatttcat     1560

attttacctg agcaaattga ttagaaaatg gaaccactag aggaatataa tgtgttagga     1620

aattacagtc atttctaagg gcccagccct tgacaaaatt gtgaagttaa attctccact     1680

ctgtccatca gatactatgg ttctccacta tggcaactaa ctcactcaat tttccctcct     1740

tagcagcatt ccatcttccc gatcttcttt gcttctccaa ccaaaacatc aatgtttatt     1800

agttctgtat acagtacagg atctttggtc tactctatca caaggccagt accacactca     1860

tgaagaaaga acacaggagt agctgagagg ctaaaactca tcaaaaacac tactcctttt     1920

cctctaccct attcctcaat cttttacctt ttccaaatcc caatccccaa atcagttttt     1980

ctctttctta ctccctctct cccttttacc ctccatggtc gttaaaggag agatggggag     2040

catcattctg ttatacttct gtacacagtt atacatgtct atcaaaccca gacttgcttc     2100

cgtagtggag acttgctttt cagaacatag ggatgaagta aggtgcctga aaagtttggg     2160

ggaaaagttt ctttcagaga gttaagttat tttatatata taatatatat ataaaatata     2220

taatatacaa tataaatata tagtgtgtgt gtatgcgtgt gtgtagacac acacgcatac     2280

acacatataa tggaagcaat aagccattct aagagcttgt atggttatgg aggtctgact     2340

aggcatgatt tcacgaaggc aagattggca tatcattgta actaaaaaag ctgacattga     2400

cccagacata ttgtactctt tctaaaaata ataataataa tgctaacaga aagaagagaa     2460

ccgttcgttt gcaatctaca gctagtagag actttgagga agaattcaac agtgtgtctt     2520

cagcagtgtt cagagccaag caagaagttg aagttgccta gaccagagga cataagtatc     2580

atgtctcctt taactagcat accccgaagt ggagaagggt gcagcaggct caaaggcata     2640

agtcattcca atcagccaac taagttgtcc ttttctggtt tcgtgttcac catggaacat     2700

tttgattata gttaatcctt ctatcttgaa tcttctagag agttgctgac caactgacgt     2760

atgtttccct ttgtgaatta ataaactggt gttctggttc at                        2802


