                         SEQUENCE LISTING

<110>  Firmenich SA
       Li, Pan
       Wang, Qi
       He, Xiu-Feng
       Haefliger, Olivier
 
<120>  SESQUITERPENE SYNTHASES FOR PRODUCTION OF DRIMENOL AND MIXTURES 
       THEREOF

<130>  10110_PCT

<150>  PCT/CN2017/082803
<151>  2017-05-03

<150>  EP 17174791.8
<151>  2017-06-07

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  1674
<212>  DNA
<213>  Paeonia anomala


<220>
<221>  misc_feature
<223>  cDNA

<400>  1
atgtctgtca aagttcctca atctcagaat gctcctacag aggttggacg tcggtccgta       60

aattttcatc ctactgtttg gggagatcgg tttatcacat acaataacca gtcagttgat      120

gatgatgtgg aaaagagatt aacaaaagaa ctaaaatccc aagtgaggag aaagttggtg      180

gatgctgctg aaaatacatg tcagaagctt aacacaatcg atgcaatcga gagattaggc      240

ttggcttatc atttcgaaac agagattgaa gaagcactgc aaaatattta taattcctct      300

caggttgttg gaaataatgt ggaagaagat gacctctact ctgttgcctt acgctttagg      360

cttctcagac aacagggcta caatatttca tctgatgtgt ttaacaaatt caaagatgat      420

aaagataact tcaaggtatc tttaattggt gatgcatcaa gcttgctaag cctatatgaa      480

gctgcacacc ttcgagtaca cggagaacac atactggatg aagctctaac tttctcagtt      540

aataatctgg aatcaatggc aacccaatta agtccacccc ttgcaacaca tgtaacccat      600

gcactaaaca gaccacttcg aaagggcatt ccaaggctag aagcaaggca ctacatttct      660

gtctacgaac aagatccttt acacgatgaa gatctattga agctcgcaaa gttagatttc      720

aaccaattac agaaaattca ccagaaggag ctaagcgaga tctcaaagtg gtggaaagat      780

ataaactttg tatcaaagct accttttgca agggacagag tggtggagtg ctacttttgg      840

ataatgtcag tgcatagcga gcccgagaac tggcttgcac gaaggacagc tgcaaaaata      900

gctgcggtaa cctccattat agatgatatc tatgatgtgc atggtacaat tgacgaactg      960

acgctattta cagaagccgt caacaggtgg gatataaaca acattgatca actcccggag     1020

tacatgaaaa tatgttataa ggcgctcttg ggcgttttta gtgaattagg ggaagagttg     1080

gaaaaacaag gaagatctta ccgcctcgat catacaattg aacttatgaa agatctagtt     1140

gggaactatt ttactgaatc gaaatggtta agcgaaaaat atgtgcccac aatagaggag     1200

tatatgcgtg ctgcagaagt caccataggt tacaacaatg ctataactgc atcttttgcc     1260

acagccaaag ccggagatat tgcaaccaag gagacctttg aatgggtgtt gagtgaacct     1320

aaaattgtta aggcttcctc agtaatttgc aggttgatgg atgacttatc atcccacaag     1380

tttgagcaaa agagaggaca tgttgcatct gctattgaat gctacatgaa gcaacatgat     1440

gctacagagg aaaaggtgcg tgcggagttt aataaacaag tcaccgacgc ctggaaggtg     1500

ataaatcaag aatgtctcca cccaacagcc attccaatgc ctcttcttac atgtgttctc     1560

aactatgcac gtgtggctga tgtcatgtac aaggatggag atgcttatac atttgcccag     1620

atcttactga aagatcattt atcggcattg ttcaccgatc ctattgcaat gtga           1674


<210>  2
<211>  557
<212>  PRT
<213>  Paeonia anomala

<400>  2

Met Ser Val Lys Val Pro Gln Ser Gln Asn Ala Pro Thr Glu Val Gly 
1               5                   10                  15      


Arg Arg Ser Val Asn Phe His Pro Thr Val Trp Gly Asp Arg Phe Ile 
            20                  25                  30          


Thr Tyr Asn Asn Gln Ser Val Asp Asp Asp Val Glu Lys Arg Leu Thr 
        35                  40                  45              


Lys Glu Leu Lys Ser Gln Val Arg Arg Lys Leu Val Asp Ala Ala Glu 
    50                  55                  60                  


Asn Thr Cys Gln Lys Leu Asn Thr Ile Asp Ala Ile Glu Arg Leu Gly 
65                  70                  75                  80  


Leu Ala Tyr His Phe Glu Thr Glu Ile Glu Glu Ala Leu Gln Asn Ile 
                85                  90                  95      


Tyr Asn Ser Ser Gln Val Val Gly Asn Asn Val Glu Glu Asp Asp Leu 
            100                 105                 110         


Tyr Ser Val Ala Leu Arg Phe Arg Leu Leu Arg Gln Gln Gly Tyr Asn 
        115                 120                 125             


Ile Ser Ser Asp Val Phe Asn Lys Phe Lys Asp Asp Lys Asp Asn Phe 
    130                 135                 140                 


Lys Val Ser Leu Ile Gly Asp Ala Ser Ser Leu Leu Ser Leu Tyr Glu 
145                 150                 155                 160 


Ala Ala His Leu Arg Val His Gly Glu His Ile Leu Asp Glu Ala Leu 
                165                 170                 175     


Thr Phe Ser Val Asn Asn Leu Glu Ser Met Ala Thr Gln Leu Ser Pro 
            180                 185                 190         


Pro Leu Ala Thr His Val Thr His Ala Leu Asn Arg Pro Leu Arg Lys 
        195                 200                 205             


Gly Ile Pro Arg Leu Glu Ala Arg His Tyr Ile Ser Val Tyr Glu Gln 
    210                 215                 220                 


Asp Pro Leu His Asp Glu Asp Leu Leu Lys Leu Ala Lys Leu Asp Phe 
225                 230                 235                 240 


Asn Gln Leu Gln Lys Ile His Gln Lys Glu Leu Ser Glu Ile Ser Lys 
                245                 250                 255     


Trp Trp Lys Asp Ile Asn Phe Val Ser Lys Leu Pro Phe Ala Arg Asp 
            260                 265                 270         


Arg Val Val Glu Cys Tyr Phe Trp Ile Met Ser Val His Ser Glu Pro 
        275                 280                 285             


Glu Asn Trp Leu Ala Arg Arg Thr Ala Ala Lys Ile Ala Ala Val Thr 
    290                 295                 300                 


Ser Ile Ile Asp Asp Ile Tyr Asp Val His Gly Thr Ile Asp Glu Leu 
305                 310                 315                 320 


Thr Leu Phe Thr Glu Ala Val Asn Arg Trp Asp Ile Asn Asn Ile Asp 
                325                 330                 335     


Gln Leu Pro Glu Tyr Met Lys Ile Cys Tyr Lys Ala Leu Leu Gly Val 
            340                 345                 350         


Phe Ser Glu Leu Gly Glu Glu Leu Glu Lys Gln Gly Arg Ser Tyr Arg 
        355                 360                 365             


Leu Asp His Thr Ile Glu Leu Met Lys Asp Leu Val Gly Asn Tyr Phe 
    370                 375                 380                 


Thr Glu Ser Lys Trp Leu Ser Glu Lys Tyr Val Pro Thr Ile Glu Glu 
385                 390                 395                 400 


Tyr Met Arg Ala Ala Glu Val Thr Ile Gly Tyr Asn Asn Ala Ile Thr 
                405                 410                 415     


Ala Ser Phe Ala Thr Ala Lys Ala Gly Asp Ile Ala Thr Lys Glu Thr 
            420                 425                 430         


Phe Glu Trp Val Leu Ser Glu Pro Lys Ile Val Lys Ala Ser Ser Val 
        435                 440                 445             


Ile Cys Arg Leu Met Asp Asp Leu Ser Ser His Lys Phe Glu Gln Lys 
    450                 455                 460                 


Arg Gly His Val Ala Ser Ala Ile Glu Cys Tyr Met Lys Gln His Asp 
465                 470                 475                 480 


Ala Thr Glu Glu Lys Val Arg Ala Glu Phe Asn Lys Gln Val Thr Asp 
                485                 490                 495     


Ala Trp Lys Val Ile Asn Gln Glu Cys Leu His Pro Thr Ala Ile Pro 
            500                 505                 510         


Met Pro Leu Leu Thr Cys Val Leu Asn Tyr Ala Arg Val Ala Asp Val 
        515                 520                 525             


Met Tyr Lys Asp Gly Asp Ala Tyr Thr Phe Ala Gln Ile Leu Leu Lys 
    530                 535                 540                 


Asp His Leu Ser Ala Leu Phe Thr Asp Pro Ile Ala Met 
545                 550                 555         


<210>  3
<211>  1674
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimized sequence

<400>  3
atgtccgtta aagttccgca aagccaaaat gcccctaccg aagttggccg tcgttccgtc       60

aacttccacc cgacggtctg gggtgatcgt ttcattacct acaataacca gagcgttgac      120

gacgatgtgg aaaagcgttt gaccaaagaa ttgaagtccc aggtccgtcg taaactggtt      180

gacgctgcag agaacacttg ccagaaactg aacaccatcg acgcgatcga gcgcctgggt      240

ctggcttacc atttcgagac tgagattgaa gaggcactgc agaacatcta caattccagc      300

caagtcgtgg gcaataatgt agaggaagat gatttatata gcgtggcgct gcgttttcgt      360

ctgctgcgtc aacagggtta taacatcagc tccgatgtct ttaacaagtt caaagatgat      420

aaagacaatt tcaaggttag cctgatcggt gacgcaagct ctttgttatc tctgtatgaa      480

gccgcgcatc tgcgcgtgca tggcgagcat atcttggatg aagcgctgac ctttagcgtt      540

aataatctgg aatcgatggc aacccagctg agcccgccgc tggcaacgca cgttacgcac      600

gcgttgaacc gcccgctgcg caagggtatc ccgcgtctgg aagcgcgtca ttacatttct      660

gtgtacgaac aagatccact gcacgacgaa gatttgctta aactggcgaa actggatttt      720

aatcaactgc aaaagattca ccagaaagaa ctgagcgaga ttagcaaatg gtggaaagac      780

attaatttcg tcagcaagct gccgttcgcc cgcgaccgtg ttgtggagtg ctatttctgg      840

attatgagcg ttcacagcga gcctgagaac tggctggcgc gccgcaccgc ggctaagatt      900

gcggcagtca cgtcgattat cgacgatatc tatgacgtcc acggcaccat cgatgaactg      960

acgctgttca ccgaagccgt taaccgctgg gacatcaaca acattgatca gctgccggaa     1020

tacatgaaga tctgctacaa agcgctgctg ggcgtgttca gcgagctggg tgaagaactg     1080

gagaaacagg gtcgtagcta tcgcttggat cataccattg agctgatgaa agatctggtc     1140

ggtaattact tcaccgagtc caagtggctg agcgagaaat acgttccgac gatcgaagag     1200

tacatgcgtg ctgccgaagt gaccatcggt tacaacaatg ccattacggc atcttttgcc     1260

acggcaaagg ccggtgatat cgctaccaaa gaaacctttg aatgggtgct gagcgaaccg     1320

aagattgtca aagcctccag cgttatttgt cgtctgatgg acgatttgag cagccataag     1380

tttgagcaaa agcgtggcca cgtcgcgagc gcgatcgagt gctatatgaa acagcacgac     1440

gcgaccgagg aaaaagttcg tgcagagttc aataaacaag tcaccgatgc gtggaaagtc     1500

attaaccaag agtgcttgca cccgacggcg atcccgatgc cactgctgac ctgtgtgctc     1560

aattatgcac gtgttgcgga cgttatgtat aaggatggtg acgcgtatac ctttgcgcaa     1620

attctgctga aagaccacct gagcgcactg ttcacggacc cgatcgcgat gtaa           1674


<210>  4
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  4
atgtctgtca aagttcctca atc                                               23


<210>  5
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  5
tcacattgca ataggatcgg tg                                                22


