                         SEQUENCE LISTING

<110>  The Broad Institute, Inc.
       Xin, Xiaofeng
       Nicol, Robert
 
<120>  METHODS FOR LINKING POLYNUCLEOTIDES

<130>  BROD-0861WP

<150>  62/490,453
<151>  2017-04-26

<160>  43    

<170>  PatentIn version 3.5

<210>  1
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SapI restriction site


<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  N = A or T or C or G

<400>  1
gctcttcn                                                                 8


<210>  2
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SapI restriction site


<220>
<221>  misc_feature
<222>  (8)..(11)
<223>  N = A or T or G or C

<400>  2
cgagaagnnn n                                                            11


<210>  3
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' phosphorylation

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (15)..(16)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (16)..(17)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (17)..(18)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (18)..(19)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (26)..(27)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (27)..(28)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (28)..(29)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (30)..(31)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (31)..(32)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (45)..(46)
<223>  Phosphorothioate bond

<400>  3
gatcggaaga gcgctggtgg taucgccacc agcgctcttc cgatct                      46


<210>  4
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  4
acactctttc cctacacgac gctcttccga tct                                    33


<210>  5
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  5
gtgactggag ttcagacgtg tgctcttccg atct                                   34


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' Azide modification followed by an internal spacer 18

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (3)..(4)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (5)..(6)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (6)..(7)
<223>  Phosphorothioate bond

<400>  6
acactctttc cctacacgac                                                   20


<210>  7
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' Hexynyl modification followed by an internal spacer 18

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Phophorothioate bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Phophorothioate bond

<220>
<221>  misc_feature
<222>  (3)..(4)
<223>  Phophorothioate bond

<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  Phophorothioate bond

<220>
<221>  misc_feature
<222>  (5)..(6)
<223>  Phophorothioate bond

<220>
<221>  misc_feature
<222>  (6)..(7)
<223>  Phophorothioate bond

<400>  7
gtgactggag ttcagacgtg t                                                 21


<210>  8
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  8
ccaccagcct gtctcttata cacatct                                           27


<210>  9
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' Phosphorylation

<400>  9
agatgtgtat aagagacagg ctggtgg                                           27


<210>  10
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  10
gaggagagat gtgtataaga gacag                                             25


<210>  11
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  11
ctgtctctta tacacatctc tcctc                                             25


<210>  12
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  12
ggcggaagat gtgtataaga gacag                                             25


<210>  13
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  13
ctgtctctta tacacatctt ccgcc                                             25


<210>  14
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' Phosphorylation

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (15)..(16)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (16)..(17)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (17)..(18)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (18)..(19)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (26)..(27)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (27)..(28)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (28)..(29)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (30)..(31)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (31)..(32)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (45)..(46)
<223>  Phosphorothioate bond

<400>  14
gatcggaaga gcgctggtgg taucgccacc agcgctcttc cgatct                      46


<210>  15
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  15
acactctttc cctacacgac gctcttccga tct                                    33


<210>  16
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  16
gtgactggag ttcagacgtg tgctcttccg atct                                   34


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' Azide modification followed by an internal spacer 18

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (3)..(4)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (5)..(6)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (6)..(7)
<223>  Phosphorothioate bond

<400>  17
acactctttc cctacacgac                                                   20


<210>  18
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' Hexynyl modification followed by an internal spacer 18

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (3)..(4)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (5)..(6)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (6)..(7)
<223>  Phosphorothioate bond

<400>  18
gtgactggag ttcagacgtg t                                                 21


<210>  19
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  19
gaggagagat gtgtataaga gacag                                             25


<210>  20
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  20
ctgtctctta tacacatctc tcctc                                             25


<210>  21
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  21
gaggagagat gtgtataaga gacag                                             25


<210>  22
<211>  92
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' Phosphorylation

<220>
<221>  misc_feature
<222>  (91)..(92)
<223>  Phosphorothioate bond

<400>  22
gatcggaaga gcgtcgtgta gggaaagagt gttutccttc ccttcccttc ccttcctuta       60

cactctttcc ctacacgacg ctcttccgat ct                                     92


<210>  23
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' Phosphorylation

<220>
<221>  misc_feature
<222>  (69)..(70)
<223>  Phosphorothioate bond

<400>  23
gatcggaaga gcgtcgtgta gggaaagagt gttaucgaca ctctttccct acacgacgct       60

cttccgatct                                                              70


<210>  24
<211>  94
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' Phosphorylation

<220>
<221>  misc_feature
<222>  (93)..(94)
<223>  Phosphorothioate bond

<400>  24
gatcggaaga gcacacgtct gaactccagt cactutggtg gtggtgggtt gttgttgtut       60

gtgactggag ttcagacgtg tgctcttccg atct                                   94


<210>  25
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' Phosphorylation

<220>
<221>  misc_feature
<222>  (71)..(72)
<223>  Phosphorothioate bond

<400>  25
gatcggaaga gcacacgtct gaactccagt cactaugcgt gactggagtt cagacgtgtg       60

ctcttccgat ct                                                           72


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  26
acactctttc cctacacgac                                                   20


<210>  27
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  27
gtgactggag ttcagacgtg t                                                 21


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' Azide modification followed by internal spacer 18

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (3)..(4)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (5)..(6)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (6)..(7)
<223>  Phosphorothioate bond

<400>  28
acactctttc cctacacgac                                                   20


<210>  29
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' Hexynyl modification followed by internal spacer 18

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (3)..(4)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (5)..(6)
<223>  Phosphorothioate bond

<220>
<221>  misc_feature
<222>  (6)..(7)
<223>  Phosphorothioate bond

<400>  29
gtgactggag ttcagacgtg t                                                 21


<210>  30
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  30
gaggagagat gtgtataaga gacag                                             25


<210>  31
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  31
ctgtctctta tacacatctc tcctc                                             25


<210>  32
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  32
gaggagagat gtgtataaga gacag                                             25


<210>  33
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  33
aggggccagt ggatagac                                                     18


<210>  34
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  34
gatggtggga agatggatac                                                   20


<210>  35
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (28)..(30)
<223>  this base is a ribonucleotide

<400>  35
aagcagtggt atcaacgcag agtacatggg                                        30


<210>  36
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  36
aagcagtggt atcaacgcag agt                                               23


<210>  37
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' Azide modification followed by internal spacer 18

<400>  37
aggggccagt ggatagac                                                     18


<210>  38
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' Hexynyl modification followed by internal spacer 18

<400>  38
gatggtggga agatggata                                                    19


<210>  39
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  39
accagcctga agagcagtgg tatcaacgca gagt                                   34


<210>  40
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  40
accagccttg agagcagtgg tatcaacgca gagt                                   34


<210>  41
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  41
accagcgtgc agagcagtgg tatcaacgca gagt                                   34


<210>  42
<211>  488
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  42
gtcctggatt cgattcccag ttcctcacat tcaggcagca ctgaacacgg acccctcacc       60

atgaacttcg ggctcagctt gattttcctt gccctcattt taaaaggtgt ccagtgtgag      120

gtgcacctgg tggagtctgg gggagactta gtgaagcctg gagggtccct gaaactctcc      180

tgtgcagcct ctggattcac tttcagtcac tatggcatgt cttgggttcg ccagactcca      240

gacaagaggc tggagtgggt cgcaaccatt ggtagtcgtg gtacttacac ccactatcca      300

gacagtgtga agggacgatt caccatctcc agagacaatg acaagaacgc cctgtacctg      360

caaatgaaca gtctgaagtc tgaagacaca gccatgtatt actgtgcaag aagaagtgaa      420

ttttattact acggtaatac ctactattac tctgctatgg actactgggg tcaaggagcc      480

tcagtcac                                                               488


<210>  43
<211>  402
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  43
ctcagagatg gagaaagaca cactcctgct atgggtcctg cttctctggg ttccaggttc       60

cacaggtgac attgtgctga cccaatctcc agcttctttg gctgtatctc taggacagag      120

ggccaccatc tcctgcagag ccagcgaaag tgttgataat tatggcttta gttttatgaa      180

ctggttccag cagaaaccag gacagccccc caaactcctc atctatgcta tatccaaccg      240

aggatccggg gtccctgcca ggtttagtgg cagtgggtct gggacagact tcagcctcaa      300

catccatcct gtagaggagg atgatcctgc aatgtatttc tgtcagcaaa ctaaggaggt      360

tccgtggacg ttcggtggag gcaccaagct ggaaatcaaa cg                         402


