                         SEQUENCE LISTING

<110>  Auckland Uniservices Limited
       Moreland, Nicole J
       Proft, Thomas J
       Young, Paul G
 
<120>  Analytical and therapeutic methods and compositions, and uses 
       thereof

<130>  306276PCT

<150>  NZ 731324
<151>  2017-04-26

<150>  NZ 736448
<151>  2017-10-16

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  540
<212>  PRT
<213>  Streptococcus pyogenes

<400>  1

Glu Ser Asn Lys Gln Asn Thr Ala Ser Thr Glu Thr Thr Thr Thr Asn 
1               5                   10                  15      


Glu Gln Pro Lys Pro Glu Ser Ser Glu Leu Thr Thr Glu Lys Ala Gly 
            20                  25                  30          


Gln Lys Thr Asp Asp Met Leu Asn Ser Asn Asp Met Ile Lys Leu Ala 
        35                  40                  45              


Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys Lys Ser 
    50                  55                  60                  


Glu Asp Lys Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile Asn Asp 
65                  70                  75                  80  


Lys Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala Lys Asn 
                85                  90                  95      


Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys Lys Ala 
            100                 105                 110         


Asp Lys Phe Ile Val Ile Glu Arg Lys Lys Lys Asn Ile Asn Thr Thr 
        115                 120                 125             


Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr Tyr Pro 
    130                 135                 140                 


Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Lys Pro Asp 
145                 150                 155                 160 


Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp Leu Pro 
                165                 170                 175     


Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr Tyr Ala 
            180                 185                 190         


Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His Asp Asn 
        195                 200                 205             


Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr Glu Ser 
    210                 215                 220                 


Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val Asn Ser 
225                 230                 235                 240 


Lys Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile Ser Lys 
                245                 250                 255     


Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe Tyr Thr 
            260                 265                 270         


Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp Lys Ser 
        275                 280                 285             


Val Thr Phe Lys Glu Leu Gln Arg Lys Gly Val Ser Asn Glu Ala Pro 
    290                 295                 300                 


Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe Val Lys 
305                 310                 315                 320 


Leu Glu Thr Ser Ser Lys Ser Asn Asp Val Glu Ala Ala Phe Ser Ala 
                325                 330                 335     


Ala Leu Lys Gly Thr Asp Val Lys Thr Asn Gly Lys Tyr Ser Asp Ile 
            340                 345                 350         


Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp Ala Ala 
        355                 360                 365             


Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Val Ile Arg Asn Val 
    370                 375                 380                 


Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr Pro Ile 
385                 390                 395                 400 


Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly Val Asn 
                405                 410                 415     


Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr Ser Gly 
            420                 425                 430         


Lys Ile Asn Leu Ser His Gln Gly Ala Tyr Val Ala Gln Tyr Glu Ile 
        435                 440                 445             


Leu Trp Asp Glu Ile Asn Tyr Asp Asp Lys Gly Lys Glu Val Ile Thr 
    450                 455                 460                 


Lys Arg Arg Trp Asp Asn Asn Trp Tyr Ser Lys Thr Ser Pro Phe Ser 
465                 470                 475                 480 


Thr Val Ile Pro Leu Gly Ala Asn Ser Arg Asn Ile Arg Ile Met Ala 
                485                 490                 495     


Arg Glu Cys Thr Gly Leu Ala Trp Glu Trp Trp Arg Lys Val Ile Asp 
            500                 505                 510         


Glu Arg Asp Val Lys Leu Ser Lys Glu Ile Asn Val Asn Ile Ser Gly 
        515                 520                 525             


Ser Thr Leu Ser Pro Tyr Gly Ser Ile Thr Tyr Lys 
    530                 535                 540 


<210>  2
<211>  540
<212>  PRT
<213>  Streptococcus pyogenes

<400>  2

Glu Ser Asn Lys Gln Asn Thr Ala Ser Thr Glu Thr Thr Thr Thr Asn 
1               5                   10                  15      


Glu Gln Pro Lys Pro Glu Ser Ser Glu Leu Thr Thr Glu Lys Ala Gly 
            20                  25                  30          


Gln Lys Thr Asp Asp Met Leu Asn Ser Asn Asp Met Ile Lys Leu Ala 
        35                  40                  45              


Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys Lys Ser 
    50                  55                  60                  


Glu Asp Lys Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile Asn Asp 
65                  70                  75                  80  


Lys Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala Lys Asn 
                85                  90                  95      


Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys Lys Ala 
            100                 105                 110         


Asp Lys Phe Ile Val Ile Glu Arg Lys Lys Lys Asn Ile Asn Thr Thr 
        115                 120                 125             


Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr Tyr Pro 
    130                 135                 140                 


Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Lys Pro Asp 
145                 150                 155                 160 


Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp Leu Pro 
                165                 170                 175     


Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr Tyr Ala 
            180                 185                 190         


Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His Asp Asn 
        195                 200                 205             


Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr Glu Ser 
    210                 215                 220                 


Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val Asn Ser 
225                 230                 235                 240 


Lys Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile Ser Lys 
                245                 250                 255     


Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe Tyr Thr 
            260                 265                 270         


Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp Lys Ser 
        275                 280                 285             


Val Thr Phe Lys Glu Leu Gln Arg Lys Gly Val Ser Asn Glu Ala Pro 
    290                 295                 300                 


Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe Val Lys 
305                 310                 315                 320 


Leu Glu Thr Ser Ser Lys Ser Asn Asp Val Glu Ala Ala Phe Ser Ala 
                325                 330                 335     


Ala Leu Lys Gly Thr Asp Val Lys Thr Asn Gly Lys Tyr Ser Asp Ile 
            340                 345                 350         


Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp Ala Ala 
        355                 360                 365             


Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Val Ile Arg Asn Val 
    370                 375                 380                 


Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Leu Ala Tyr Pro Ile 
385                 390                 395                 400 


Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly Val Asn 
                405                 410                 415     


Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr Ser Gly 
            420                 425                 430         


Lys Ile Asn Leu Ser His Gln Gly Ala Tyr Val Ala Gln Tyr Glu Ile 
        435                 440                 445             


Leu Trp Asp Glu Ile Asn Tyr Asp Asp Lys Gly Lys Glu Val Ile Thr 
    450                 455                 460                 


Lys Arg Arg Trp Asp Asn Asn Trp Tyr Ser Lys Thr Ser Pro Phe Ser 
465                 470                 475                 480 


Thr Val Ile Pro Leu Gly Ala Asn Ser Arg Asn Ile Arg Ile Met Ala 
                485                 490                 495     


Arg Glu Cys Thr Gly Leu Ala Phe Glu Trp Trp Arg Lys Val Ile Asp 
            500                 505                 510         


Glu Arg Asp Val Lys Leu Ser Lys Glu Ile Asn Val Asn Ile Ser Gly 
        515                 520                 525             


Ser Thr Leu Ser Pro Tyr Gly Ser Ile Thr Tyr Lys 
    530                 535                 540 


<210>  3
<211>  35
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic - oligonucleotide synthesized in laboratory

<400>  3
caccatgcga caaacacagg tctcaaatga tgttg                                  35


<210>  4
<211>  35
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic - oligonucleotide synthesized in laboratory

<400>  4
tttctgagta ggtgtaccgt tatggtagtt aatgg                                  35


<210>  5
<211>  229
<212>  PRT
<213>  Streptococcus pyogenes

<400>  5

Arg Gln Thr Gln Val Ser Asn Asp Val Val Leu Asn Asp Gly Ala Ser 
1               5                   10                  15      


Lys Tyr Leu Asn Glu Ala Leu Ala Trp Thr Phe Asn Asp Ser Pro Asn 
            20                  25                  30          


Tyr Tyr Lys Thr Leu Gly Thr Ser Gln Ile Thr Pro Ala Leu Phe Pro 
        35                  40                  45              


Lys Ala Gly Asp Ile Leu Tyr Ser Lys Leu Asp Glu Leu Gly Arg Thr 
    50                  55                  60                  


Arg Thr Ala Arg Gly Thr Leu Thr Tyr Ala Asn Val Glu Gly Ser Tyr 
65                  70                  75                  80  


Gly Val Arg Gln Ser Phe Gly Lys Asn Gln Asn Pro Ala Gly Trp Thr 
                85                  90                  95      


Gly Asn Pro Asn His Val Lys Tyr Lys Ile Glu Trp Leu Asn Gly Leu 
            100                 105                 110         


Ser Tyr Val Gly Asp Phe Trp Asn Arg Ser His Leu Ile Ala Asp Ser 
        115                 120                 125             


Leu Gly Gly Asp Ala Leu Arg Val Asn Ala Val Thr Gly Thr Arg Thr 
    130                 135                 140                 


Gln Asn Val Gly Gly Arg Asp Gln Lys Gly Gly Met Arg Tyr Thr Glu 
145                 150                 155                 160 


Gln Arg Ala Gln Glu Trp Leu Glu Ala Asn Arg Asp Gly Tyr Leu Tyr 
                165                 170                 175     


Tyr Glu Ala Ala Pro Ile Tyr Asn Ala Asp Glu Leu Ile Pro Arg Ala 
            180                 185                 190         


Val Val Val Ser Met Gln Ser Ser Asp Asn Thr Ile Asn Glu Lys Val 
        195                 200                 205             


Leu Val Tyr Asn Thr Ala Asn Gly Tyr Thr Ile Asn Tyr His Asn Gly 
    210                 215                 220                 


Thr Pro Thr Gln Lys 
225                 


<210>  6
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic - oligonucleotide synthesized in laboratory

<400>  6
aaaggcgccc gccaaaattt gacttatgcc aa                                     32


<210>  7
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic - oligonucleotide synthesized in laboratory

<400>  7
aaactcgagc tatttggaaa atgataattg aagtaaca                               38


<210>  8
<211>  827
<212>  PRT
<213>  Streptococcus pyogenes

<400>  8

Arg Gln Asn Leu Thr Tyr Ala Asn Glu Ile Val Thr Gln Arg Pro Lys 
1               5                   10                  15      


Arg Glu Ser Val Ile Ser Asp Lys Ser Asn Phe Pro Val Ile Ser Pro 
            20                  25                  30          


Tyr Leu Ala Ser Val Asp Phe Gly Glu Arg Lys Thr Pro Leu Pro Thr 
        35                  40                  45              


Pro Asp Lys Gly Val Lys Val Thr Thr Glu Gln Ser Ile Ala Gln Val 
    50                  55                  60                  


Arg Lys Gly Pro Glu Glu Arg Pro Tyr Thr Val Thr Gly Lys Ile Thr 
65                  70                  75                  80  


Ser Val Ile Asn Gly Trp Gly Gly Tyr Gly Phe Tyr Ile Gln Asp Ser 
                85                  90                  95      


Glu Gly Ile Gly Leu Tyr Val Tyr Pro Gln Lys Asp Leu Gly Tyr Ser 
            100                 105                 110         


Lys Gly Asp Ile Val Gln Leu Thr Gly Thr Leu Thr Arg Phe Lys Gly 
        115                 120                 125             


Asp Leu Gln Leu Gln Gln Val Thr Ala His Lys Lys Leu Glu Leu Ser 
    130                 135                 140                 


Phe Pro Thr Ser Val Lys Glu Ala Val Ile Ser Glu Leu Glu Thr Thr 
145                 150                 155                 160 


Thr Pro Ser Thr Leu Val Lys Leu Ser His Val Thr Val Gly Glu Leu 
                165                 170                 175     


Ser Thr Asp Gln Tyr Asn Asn Thr Ser Phe Leu Val Arg Asp Asp Ser 
            180                 185                 190         


Gly Lys Ser Ile Val Val His Ile Asp His Arg Thr Gly Val Lys Gly 
        195                 200                 205             


Ala Asp Val Val Thr Lys Ile Ser Gln Gly Asp Leu Ile Asn Leu Thr 
    210                 215                 220                 


Ala Ile Leu Ser Ile Val Asp Gly Gln Leu Gln Leu Arg Pro Phe Ser 
225                 230                 235                 240 


Leu Glu Gln Leu Glu Val Val Lys Lys Val Thr Ser Ser Asn Ser Asp 
                245                 250                 255     


Ala Ser Ser Arg Asn Ile Val Lys Ile Gly Glu Ile Gln Gly Ala Ser 
            260                 265                 270         


His Thr Ser Pro Leu Leu Lys Lys Ala Val Thr Val Glu Gln Val Val 
        275                 280                 285             


Val Thr Tyr Leu Asp Asp Ser Thr His Phe Tyr Val Gln Asp Leu Asn 
    290                 295                 300                 


Gly Asp Gly Asp Leu Ala Thr Ser Asp Gly Ile Arg Val Phe Ala Lys 
305                 310                 315                 320 


Asn Ala Lys Val Gln Val Gly Asp Val Leu Thr Ile Ser Gly Glu Val 
                325                 330                 335     


Glu Glu Phe Phe Gly Arg Gly Tyr Glu Glu Arg Lys Gln Thr Asp Leu 
            340                 345                 350         


Thr Ile Thr Gln Ile Val Ala Lys Ala Val Thr Lys Thr Gly Thr Ala 
        355                 360                 365             


Gln Val Pro Ser Pro Leu Val Leu Gly Lys Asp Arg Ile Ala Pro Ala 
    370                 375                 380                 


Asn Ile Ile Asp Asn Asp Gly Leu Arg Val Phe Asp Pro Glu Glu Asp 
385                 390                 395                 400 


Ala Ile Asp Tyr Trp Glu Ser Met Glu Gly Met Leu Val Ala Val Asp 
                405                 410                 415     


Asp Ala Lys Ile Leu Gly Pro Met Lys Asn Lys Glu Ile Tyr Val Leu 
            420                 425                 430         


Pro Gly Ser Ser Thr Arg Pro Leu Asn Asn Ser Gly Gly Val Leu Leu 
        435                 440                 445             


Pro Ala Asn Ser Tyr Asn Thr Asp Val Ile Pro Val Leu Phe Lys Lys 
    450                 455                 460                 


Gly Lys Gln Ile Ile Lys Ala Gly Asp Ser Tyr Lys Gly Arg Leu Ala 
465                 470                 475                 480 


Gly Pro Val Ser Tyr Ser Tyr Gly Asn Tyr Lys Val Phe Val Asp Asp 
                485                 490                 495     


Ser Lys Asn Met Pro Ser Leu Met Asp Gly His Leu Lys Pro Glu Lys 
            500                 505                 510         


Thr Asn Leu Gln Lys Asp Leu Ser Lys Leu Ser Ile Ala Ser Tyr Asn 
        515                 520                 525             


Ile Glu Asn Phe Ser Ala Asn Pro Ser Ser Thr Lys Asp Glu Lys Val 
    530                 535                 540                 


Lys Arg Ile Ala Glu Ser Phe Ile His Asp Leu Asn Ala Pro Asp Ile 
545                 550                 555                 560 


Ile Gly Leu Ile Glu Val Gln Asp Asn Asn Gly Pro Thr Asp Asp Gly 
                565                 570                 575     


Thr Thr Asp Ala Thr Gln Ser Ala Gln Arg Leu Ile Asp Ala Ile Lys 
            580                 585                 590         


Lys Leu Gly Gly Pro Thr Tyr Arg Tyr Val Asp Ile Ala Pro Glu Asn 
        595                 600                 605             


Asn Val Asp Gly Gly Gln Pro Gly Gly Asn Ile Arg Thr Gly Phe Leu 
    610                 615                 620                 


Tyr Gln Pro Glu Arg Val Ser Leu Ser Asp Lys Pro Lys Gly Gly Ala 
625                 630                 635                 640 


Arg Asp Ala Leu Thr Trp Val Asn Gly Glu Leu Asn Leu Ser Val Gly 
                645                 650                 655     


Arg Ile Asp Pro Thr Asn Ala Ala Trp Lys Asp Val Arg Lys Ser Leu 
            660                 665                 670         


Ala Ala Glu Phe Ile Phe Gln Gly Arg Lys Val Val Val Val Ala Asn 
        675                 680                 685             


His Leu Asn Ser Lys Arg Gly Asp Asn Ala Leu Tyr Gly Cys Val Gln 
    690                 695                 700                 


Pro Val Thr Phe Lys Ser Glu Gln Arg Arg His Val Leu Ala Asn Met 
705                 710                 715                 720 


Leu Ala Gln Phe Ala Lys Glu Gly Ala Lys His Gln Ala Asn Ile Val 
                725                 730                 735     


Met Leu Gly Asp Phe Asn Asp Phe Glu Phe Thr Lys Thr Ile Gln Leu 
            740                 745                 750         


Ile Glu Glu Gly Asp Met Val Asn Leu Val Ser Arg His Asp Ile Ser 
        755                 760                 765             


Asp Arg Tyr Ser Tyr Phe His Gln Gly Asn Asn Gln Thr Leu Asp Asn 
    770                 775                 780                 


Ile Leu Val Ser Arg His Leu Leu Asp His Tyr Glu Phe Asp Met Val 
785                 790                 795                 800 


His Val Asn Ser Pro Phe Met Glu Ala His Gly Arg Ala Ser Asp His 
                805                 810                 815     


Asp Pro Leu Leu Leu Gln Leu Ser Phe Ser Lys 
            820                 825         


