SEQUENCE LIST

<110>
Robert Penchovsky

<120>
Methods for creating novel antibacterial agents using chimeric antisense oligonucleotides

<141>
20 April 2017

<210>
SEQ ID NO: 1

<211>
18 amino acid residues  + 16 nucleotides

<212>
Chimeric pVEC-PS_DNA (Phosphorothioate DNA)

<213>
Artificial sequence

<220>
Antisense oligonucleotide

<223>
Antibacterial properties; The down case 1 stands for  2'-O-CH3 of the DNA oligomers while the down case 2 stands PS-modification of DNA the DNA oligomers

<400>
Lys Ser His Ala His Ala Gln Lys Arg Ile Arg Arg Arg Leu Ile Ile                        16
     Leu Leu                                                                                18
T1T1C1T2C2C2C2A2T2C2     C2A2G2A1C1T1                                                       16    
                                         

<210>
SEQ ID NO: 2

<211>
18 amino acid residues  +16 nucleotides

<212>
Chimeric pVEC-PS_DNA (Phosphorothioate DNA)

<213>
Artificial sequence

<220>
Antisense oligonucleotide

<223>
Antibacterial properties; The down case 1 stands for  2'-O-CH3 of the DNA oligomers while the down case 2 stands PS-modification of DNA the DNA oligomers

<400>
Lys Ser His Ala His Ala Gln Lys Arg Ile Arg Arg Arg Leu Ile Ile                        16
Leu Leu                                                                                     18
C1T1T1T2A2A2C2T2G2T2    A2C2T2G1C1C1                                                        16


<210>
SEQ ID NO: 3

<211>
18 amino acid residues  + 14 nucleotides
<212>
Chimeric pVEC-PS_DNA (phosphorothioate DNA)

<213>
Artificial sequence
<220>
Antisense oligonucleotide

<223>
Antibacterial properties; The down case 1 stands for  2'-O-CH3 of the DNA oligomers while the down case 2 stands PS-modification of DNA the DNA oligomers

<400>
Lys Ser His Ala His Ala Gln Lys Arg Ile Arg Arg Arg Leu Ile Ile                        16
Leu Leu                                                                                     18
T1C1C1C2T2C2C2A2C2C2     A2C1T1C1                                                           14


<210>
SEQ ID NO: 4

<211>
18 amino acid residues  + 12 nucleotides

<212>
Chimeric pVEC-PS_DNA (Phosphorothioate DNA)
<213>
Artificial sequence

<220>
Antisense oligonucleotide

<223>
Antibacterial properties; The down case 1 stands for  2'-O-CH3 of the DNA oligomers while the down case 2 stands PS-modification of DNA the DNA oligomers

<400>
Lys Ser His Ala His Ala Gln Lys Arg Ile Arg Arg Arg Leu Ile Ile                        16
Leu Leu                                                                                     18
C1A1A2T2C2C2C2T2A2C1      G1T1                                                              12


<210>
SEQ ID NO: 5

<211>
18 amino acid residues  + 16 nucleotides

<212>
Chimeric pVEC-PNA (Peptide Nucleic Acid)

<213>
Artificial sequence
<220>
Antisense oligonucleotide

<223>
Antibacterial properties

<400>
Lys Ser His Ala His Ala Gln Lys Arg Ile Arg Arg Arg Leu Ile Ile                        16
Leu Leu                                                                                     18
TTCTCCCAT CCAGACT                                                                           16


<210>
SEQ ID NO: 6

<211>
18 amino acid residues + 14 nucleotides

<212>
Chimeric pVEC-PNA (Peptide Nucleic Acid)

<213>
Artificial sequence

<220>
Antisense oligonucleotide

<223>
Antibacterial properties

<400>
Lys Ser His Ala His Ala Gln Lys Arg Ile Arg Arg Arg Leu Ile Ile                        16 
Leu Leu                                                                                     18
TCCCTCCACC    ACTC                                                                          14


<210>
SEQ ID NO: 7

<211>
18 amino acid residues  + 16 nucleotides

<212>
Chimeric pVEC-PNA (Peptide Nucleic Acid)

<213>
Artificial sequence

<220>
Antisense oligonucleotide

<223>
Antibacterial properties

<400>
Lys Ser His Ala His Ala Gln Lys Arg Ile Arg Arg Arg Leu Ile Ile                        16
Leu Leu                                                                                     18
CTTTAACTGT   ACTGCC                                                                         16


<210>
SEQ ID NO: 8

<211>
18 amino acid residues  + 12 nucleotides

<212>
Chimeric pVEC-PNA (Peptide Nucleic Acid)

<213>
Artificial sequence

<220>
Antisense oligonucleotide

<223>
Antibacterial properties

<400>
Lys Ser His Ala His Ala Gln Lys Arg Ile Arg Arg Arg Leu Ile Ile                        16
Leu Leu                                                                                     18
CAATCCCTAC   GT                                                                             12


<210>
SEQ ID NO: 9

<211>
18 amino acid residues  + 16 nucleotides

<212>
Chimeric  pVEC-LNA (Locked Nucleic Acid)

<213>
Artificial sequence
<220>
Antisense oligonucleotide

<223>
Antibacterial properties

<400>
Lys Ser His Ala His Ala Gln Lys Arg Ile Arg Arg Arg Leu Ile Ile                        16 
Leu Leu                                                                                     18 
TTCTCCCATC   CAGACT                                                                         16


<210>
SEQ ID NO: 10

<211>
18 amino acid residues  + 14 nucleotides

<212>
Chimeric pVEC-LNA (Locked Nucleic Acid)
<213>
Artificial sequence

<220>
Antisense oligonucleotide
<223>
Antibacterial properties

<400>
Lys Ser His Ala His Ala Gln Lys Arg Ile Arg Arg Arg Leu Ile Ile                        16
Leu Leu                                                                                     18
TCCCTCCACC   ACTC                                                                           14


<210>
SEQ ID NO: 11

<211>
18 amino acid residues  + 16 nucleotides

<212>
Chimeric pVEC-LNA (Locked Nucleic Acid)

<213>
Artificial sequence

<220>
Antisense oligonucleotide

<223>
Antibacterial properties

<400>
Lys Ser His Ala His Ala Gln Lys Arg Ile Arg Arg Arg Leu Ile Ile                        16
Leu Leu                                                                                     18
CTTTAACTGT  ACTGCC                                                                          16


<210>
SEQ ID NO: 12

<211>
18 amino acid residues  + 12 nucleotides

<212>
Chimeric pVEC-LNA (Locked Nucleic Acid)

<213>
Artificial sequence

<220>
Antisense oligonucleotide

<223>
Antibacterial properties

<400>
Lys Ser His Ala His Ala Gln Lys Arg Ile Arg Arg Arg Leu Ile Ile                        16
Leu Leu                                                                                     18 
CAATCCCTAC  GT                                                                              12


<210>
SEQ ID NO: 13

<211>
18 amino acid residues  + 22 nucleotides

<212>
Chimeric pVEC-PS_DNA (Phosphorothioate DNA)

<213>
Artificial sequence

<220>
Antisense oligonucleotide

<223>
Antibacterial properties; The down case 1 stands for  2'-O-CH3 of the DNA oligomers while the down case 2 stands PS-modification of DNA the DNA oligomers

<400>
Lys Ser His Ala His Ala Gln Lys Arg Ile Arg Arg Arg Leu Ile Ile                        16 
Leu Leu                                                                                     18
G1A1T1T2T2T2G2C2T2T2   C2T2T2T2A2C2C2T2A2A1   T1T1                                          22


<210>
SEQ ID NO: 14

<211>
18 amino acid residues  + 22 nucleotides

<212>
Chimeric pVEC-PS_DNA (Phosphorothioate DNA)

<213>
Artificial sequence

<220>
Antisense oligonucleotide

<223>
Antibacterial properties

<400>
Lys Ser His Ala His Ala Gln Lys Arg Ile Arg Arg Arg Leu Ile Ile                        16
Leu Leu                                                                                     18
GATTTTGCTT  CTTTACCTAA  TT                                                                  22


<210>
SEQ ID NO: 15

<211>
18 amino acid residues  + 22 nucleotides

<212>
Chimeric pVEC-PS_DNA (Phosphorothioate DNA)

<213>
Artificial sequence
<220>
Antisense oligonucleotide

<223>
Antibacterial properties

<400>
Lys Ser His Ala His Ala Gln Lys Arg Ile Arg Arg Arg Leu Ile Ile                        16
Leu Leu                                                                                     18
GATTTTGCTT   CTTTACCTAA  TT                                                                 22
 

<210>
SEQ ID NO: 16

<211>
18 amino acid residues  + 22 nucleotides

<212>
Chimeric pVEC-PS_DNA (Phosphorothioate DNA)

<213>
Artificial sequence

<220>
Antisense oligonucleotide

<223>
Antibacterial properties; The down case 1 stands for  2'-O-CH3 of the DNA oligomers while the down case 2 stands PS-modification of DNA the DNA oligomers

<400>
Lys Ser His Ala His Ala Gln Lys Arg Ile Arg Arg Arg Leu Ile Ile                        16 
Leu Leu                                                                                     18
C1A1A1C2T2T2C2T2T2T2    T2C2T2T2G2C2T2T2C2A1   T1T1                                         22       
                          

<210>
SEQ ID NO: 17

<211>
18 amino acid residues  + 22 nucleotides

<212>
Chimeric pVEC-PS_DNA (Phosphorothioate DNA)

<213>
Artificial sequence

<220>
Antisense oligonucleotide

<223>
Antibacterial properties

<400>
Lys Ser His Ala His Ala Gln Lys Arg Ile Arg Arg Arg Leu Ile Ile                        16
Leu Leu                                                                                     18
CAACTTCTTT   TCTTGCTTCA   TT                                                                22


<210>
SEQ ID NO: 18

<211>
18 amino acid residues  + 22 nucleotides

<212>
Chimeric pVEC-PS_DNA (Phosphorothioate DNA)

<213>
Artificial sequence

<220>
Antisense oligonucleotide

<223>
Antibacterial properties

<400>
Lys Ser His Ala His Ala Gln Lys Arg Ile Arg Arg Arg Leu Ile Ile                        16
Leu Leu                                                                                     18
CAACTTCTTT  TCTTGCTTCA  TT                                                                  22


<210>
SEQ ID NO: 19

<211>
24 amino acid residues

<212>
Oligopeptide

<213>
Bovine neutrophils

<220>
Cell- penetrating peptide for antisense delivery in the cell

<400>
Gly Pro Arg Pro Phe Pro Leu Pro Arg Pro Arg Pro Arg Pro Leu Arg                        16 
Pro Pro Arg Pro Arg Ile Arg Arg                                                             24


<210>
SEQ ID NO: 20

<211>
20 amino acid residues

<212>
Oligopeptide

<213>
Pyrrhocoris apterus

<220>
Cell- penetrating peptide for antisense delivery in the cell

<400>
Asn Arg Asn Tyr Ile Pro Arg Pro Pro Thr Pro Arg Pro Leu Tyr Ser                        16
Gly Lys Asp Val                                                                             20


<210>
SEQ ID NO: 21

<211>
26 amino acid residues 

<212>
Oligopeptide

<213>
Bee venom

<220>
Cell- penetrating peptide for antisense delivery in the cell

<400>
Gln Gln Arg Lys Arg  Lys Ile Trp Ser Ile Leu Ala Pro Leu Gly Thr                       16
Thr Leu Val Lys Leu Val Ala  Gly Ile Gly                                                    26


<210>
SEQ ID NO: 22

<211>
38 amino acid residues

<212>
Oligopeptide

<213>
Human host defense peptide

<220>
Cell- penetrating peptide for antisense delivery in the cell

<400>
Cys Ser Glu Thr Arg Pro Val Leu Asn Arg Leu Phe Asp Lys Ile Arg                        16 
Gln Val Ile Arg Lys Phe Glu Lys Gly Ile Lys Glu Lys Ser  Lys Arg                            32
Phe Phe Asp Gly Leu Leu                                                                     38


<210>
SEQ ID NO: 23

<211>
18 amino acid residues 

<212>
Oligopeptide

<213>
Viral origin

<220>
Cell- penetrating peptide for antisense delivery in the cell

<400>
Arg Gly Thr Ser Thr Ser Phe Arg Arg Arg Ser Tyr Ser Leu Arg Gly                        16
Gly Arg                                                                                     18


<210>
SEQ ID NO: 24

<211>
14 amino acid residues  

<212>
Oligopeptide

<213>
Crotalus durissus terrificus

<220>
Cell- penetrating peptide for antisense delivery in the cell

<400>
Gly Ser Gly Lys Lys Gly Gly Lys Lys His Cys Gln Lys Tyr                                14


<210>
SEQ ID NO: 25

<211>
20 amino acid residues

<212>
Oligopeptide

<213>
Viral origin

<220>
Cell- penetrating peptide for antisense delivery in the cell

<400>
Val Lys Arg Lys Lys Lys Pro Ala Lys Leu Val Lys Lys Leu Leu Thr                        16
Lys Trp Leu Ala                                                                             20


<210>
SEQ ID NO: 26

<211>
9 amino acid residues

<212>
Oligopeptide

<213>
Viral origin

<220>
Cell- penetrating peptide for antisense delivery in the cell

<400>
Arg Arg Arg Gln Arg Arg Lys Lys Arg                                                    9


<210>SEQ ID NO: 27
<211>18 amino acid residues


<212>
Oligopeptide

<213>
Viral origin

<220>
Cell- penetrating peptide for antisense delivery in the cell

<400>
Lys Ser His Ala His Ala Gln Lys Arg Ile Arg Arg Arg Leu Ile Ile                        16 
Leu Leu                                                                                     18


<210>
SEQ ID NO: 28

<211>
21 amino acid residues

<212>
Oligopeptide

<213>
Artificial origin 

<220>
Cell- penetrating peptide for antisense delivery in the cell

<400>
Val Lys Arg Lys Lys Lys Pro Gln Ser Trp Lys Thr Trp Trp Thr Lys                        16
Trp Trp Thr Lys Lys                                                                         21


<210>
SEQ ID NO: 29

<211>
20 amino acid residues

<212>
Oligopeptide

<213>
Viral origin

<220>
Cell- penetrating peptide for antisense delivery in the cell

<400>
Val Lys Arg Lys Lys Lys Pro Ala Lys Leu Val Lys Lys Leu Leu Thr                        16
Lys Trp Leu Ala                                                                             20




