                         SEQUENCE LISTING

<110>  Roche Diagnostics GmbH, F. Hoffmann-La Roche AG, Roche Diagnostics Operations, Inc.
 
<120>  Soluble and immunoreactive Zika virus NS1 polypeptides

<130>  P34732-WO-IR

<150>  EP17168197.6
<151>  2017-04-26

<160>  35    

<170>  PatentIn version 3.5

<210>  1
<211>  151
<212>  PRT
<213>  Artificial

<220>
<223>  Zika virus NS1 wing domain aa 30-180 with position 179 X = A or S
       or C


<220>
<221>  misc_feature
<222>  (150)..(150)
<223>  Xaa can be Ala or Ser or Cys

<400>  1

Asp Arg Tyr Lys Tyr His Pro Asp Ser Pro Arg Arg Leu Ala Ala Ala 
1               5                   10                  15      


Val Lys Gln Ala Trp Glu Glu Gly Ile Cys Gly Ile Ser Ser Val Ser 
            20                  25                  30          


Arg Met Glu Asn Ile Met Trp Lys Ser Val Glu Gly Glu Leu Asn Ala 
        35                  40                  45              


Ile Leu Glu Glu Asn Gly Val Gln Leu Thr Val Val Val Gly Ser Val 
    50                  55                  60                  


Lys Asn Pro Met Trp Arg Gly Pro Gln Arg Leu Pro Val Pro Val Asn 
65                  70                  75                  80  


Glu Leu Pro His Gly Trp Lys Ala Trp Gly Lys Ser Tyr Phe Val Arg 
                85                  90                  95      


Ala Ala Lys Thr Asn Asn Ser Phe Val Val Asp Gly Asp Thr Leu Lys 
            100                 105                 110         


Glu Cys Pro Leu Lys His Arg Ala Trp Asn Ser Phe Leu Val Glu Asp 
        115                 120                 125             


His Gly Phe Gly Val Phe His Thr Ser Val Trp Leu Lys Val Arg Glu 
    130                 135                 140                 


Asp Tyr Ser Leu Glu Xaa Asp 
145                 150     


<210>  2
<211>  151
<212>  PRT
<213>  Artificial

<220>
<223>  Zika virus NS1 wing domain aa 30-180 with C55, C143, C179A

<400>  2

Asp Arg Tyr Lys Tyr His Pro Asp Ser Pro Arg Arg Leu Ala Ala Ala 
1               5                   10                  15      


Val Lys Gln Ala Trp Glu Glu Gly Ile Cys Gly Ile Ser Ser Val Ser 
            20                  25                  30          


Arg Met Glu Asn Ile Met Trp Lys Ser Val Glu Gly Glu Leu Asn Ala 
        35                  40                  45              


Ile Leu Glu Glu Asn Gly Val Gln Leu Thr Val Val Val Gly Ser Val 
    50                  55                  60                  


Lys Asn Pro Met Trp Arg Gly Pro Gln Arg Leu Pro Val Pro Val Asn 
65                  70                  75                  80  


Glu Leu Pro His Gly Trp Lys Ala Trp Gly Lys Ser Tyr Phe Val Arg 
                85                  90                  95      


Ala Ala Lys Thr Asn Asn Ser Phe Val Val Asp Gly Asp Thr Leu Lys 
            100                 105                 110         


Glu Cys Pro Leu Lys His Arg Ala Trp Asn Ser Phe Leu Val Glu Asp 
        115                 120                 125             


His Gly Phe Gly Val Phe His Thr Ser Val Trp Leu Lys Val Arg Glu 
    130                 135                 140                 


Asp Tyr Ser Leu Glu Ala Asp 
145                 150     


<210>  3
<211>  352
<212>  PRT
<213>  Zika virus

<400>  3

Asp Val Gly Cys Ser Val Asp Phe Ser Lys Arg Glu Thr Arg Cys Gly 
1               5                   10                  15      


Thr Gly Val Phe Ile Tyr Asn Asp Val Glu Ala Trp Arg Asp Arg Tyr 
            20                  25                  30          


Lys Tyr His Pro Asp Ser Pro Arg Arg Leu Ala Ala Ala Val Lys Gln 
        35                  40                  45              


Ala Trp Glu Glu Gly Ile Cys Gly Ile Ser Ser Val Ser Arg Met Glu 
    50                  55                  60                  


Asn Ile Met Trp Lys Ser Val Glu Gly Glu Leu Asn Ala Ile Leu Glu 
65                  70                  75                  80  


Glu Asn Gly Val Gln Leu Thr Val Val Val Gly Ser Val Lys Asn Pro 
                85                  90                  95      


Met Trp Arg Gly Pro Gln Arg Leu Pro Val Pro Val Asn Gly Leu Pro 
            100                 105                 110         


His Gly Trp Lys Ala Trp Gly Lys Ser Tyr Phe Val Arg Ala Ala Lys 
        115                 120                 125             


Thr Asn Asn Ser Phe Val Val Asp Gly Asp Thr Leu Lys Glu Cys Pro 
    130                 135                 140                 


Leu Lys His Arg Ala Trp Asn Ser Phe Leu Val Glu Asp His Gly Phe 
145                 150                 155                 160 


Gly Val Phe His Thr Ser Val Trp Leu Lys Val Arg Glu Asp Tyr Ser 
                165                 170                 175     


Leu Glu Cys Asp Pro Ala Val Ile Gly Thr Ala Val Lys Gly Arg Glu 
            180                 185                 190         


Ala Ala His Ser Asp Leu Gly Tyr Trp Ile Glu Ser Glu Lys Asn Asp 
        195                 200                 205             


Thr Trp Arg Leu Lys Arg Ala His Leu Ile Glu Met Lys Thr Cys Glu 
    210                 215                 220                 


Trp Pro Lys Ser His Thr Leu Trp Thr Asp Gly Val Glu Glu Ser Asp 
225                 230                 235                 240 


Leu Ile Ile Pro Lys Ser Leu Ala Gly Pro Leu Ser His His Asn Thr 
                245                 250                 255     


Arg Glu Gly Tyr Arg Thr Gln Val Lys Gly Pro Trp His Ser Glu Glu 
            260                 265                 270         


Leu Glu Ile Arg Phe Glu Glu Cys Pro Gly Thr Lys Val His Val Glu 
        275                 280                 285             


Glu Thr Cys Gly Thr Arg Gly Pro Ser Leu Arg Ser Thr Thr Ala Ser 
    290                 295                 300                 


Gly Arg Val Ile Glu Glu Trp Cys Cys Arg Glu Cys Thr Met Pro Pro 
305                 310                 315                 320 


Leu Ser Phe Arg Ala Lys Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg 
                325                 330                 335     


Pro Arg Lys Glu Pro Glu Ser Asn Leu Val Arg Ser Met Val Thr Ala 
            340                 345                 350         


<210>  4
<211>  162
<212>  PRT
<213>  Zika virus

<400>  4

Arg Glu Ala Ala His Ser Asp Leu Gly Tyr Trp Ile Glu Ser Glu Lys 
1               5                   10                  15      


Asn Asp Thr Trp Arg Leu Lys Arg Ala His Leu Ile Glu Met Lys Thr 
            20                  25                  30          


Cys Glu Trp Pro Lys Ser His Thr Leu Trp Thr Asp Gly Val Glu Glu 
        35                  40                  45              


Ser Asp Leu Ile Ile Pro Lys Ser Leu Ala Gly Pro Leu Ser His His 
    50                  55                  60                  


Asn Thr Arg Glu Gly Tyr Arg Thr Gln Val Lys Gly Pro Trp His Ser 
65                  70                  75                  80  


Glu Glu Leu Glu Ile Arg Phe Glu Glu Cys Pro Gly Thr Lys Val His 
                85                  90                  95      


Val Glu Glu Thr Cys Gly Thr Arg Gly Pro Ser Leu Arg Ser Thr Thr 
            100                 105                 110         


Ala Ser Gly Arg Val Ile Glu Glu Trp Cys Cys Arg Glu Cys Thr Met 
        115                 120                 125             


Pro Pro Leu Ser Phe Arg Ala Lys Asp Gly Cys Trp Tyr Gly Met Glu 
    130                 135                 140                 


Ile Arg Pro Arg Lys Glu Pro Glu Ser Asn Leu Val Arg Ser Met Val 
145                 150                 155                 160 


Thr Ala 
        


<210>  5
<211>  151
<212>  PRT
<213>  Artificial

<220>
<223>  Tick-borne encephalitis (FSME) virus NS1 wing domain aa 30-180 
       according to UniProt ID P14336; European subtype strain 
       Neudoerfl; X = A or C or S


<220>
<221>  misc_feature
<222>  (150)..(150)
<223>  Xaa can be Ala or Ser or Cys

<400>  5

Asp Asn Tyr Ala Tyr Tyr Pro Glu Thr Pro Gly Ala Leu Ala Ser Ala 
1               5                   10                  15      


Ile Lys Glu Thr Phe Glu Glu Gly Ser Cys Gly Val Val Pro Gln Asn 
            20                  25                  30          


Arg Leu Glu Met Ala Met Trp Arg Ser Ser Val Thr Glu Leu Asn Leu 
        35                  40                  45              


Ala Leu Ala Glu Gly Glu Ala Asn Leu Thr Val Val Val Asp Lys Phe 
    50                  55                  60                  


Asp Pro Thr Asp Tyr Arg Gly Gly Val Pro Gly Leu Leu Lys Lys Gly 
65                  70                  75                  80  


Lys Asp Ile Lys Val Ser Trp Lys Ser Trp Gly His Ser Met Ile Trp 
                85                  90                  95      


Ser Ile Pro Glu Ala Pro Arg Arg Phe Met Val Gly Thr Glu Gly Gln 
            100                 105                 110         


Ser Glu Cys Pro Leu Glu Arg Arg Lys Thr Gly Val Phe Thr Val Ala 
        115                 120                 125             


Glu Phe Gly Val Gly Leu Arg Thr Lys Val Phe Leu Asp Phe Arg Gln 
    130                 135                 140                 


Glu Pro Thr His Glu Xaa Asp 
145                 150     


<210>  6
<211>  352
<212>  PRT
<213>  Tick-borne encephalitis virus

<400>  6

Asp Val Gly Cys Ala Val Asp Thr Glu Arg Met Glu Leu Arg Cys Gly 
1               5                   10                  15      


Glu Gly Leu Val Val Trp Arg Glu Val Ser Glu Trp Tyr Asp Asn Tyr 
            20                  25                  30          


Ala Tyr Tyr Pro Glu Thr Pro Gly Ala Leu Ala Ser Ala Ile Lys Glu 
        35                  40                  45              


Thr Phe Glu Glu Gly Ser Cys Gly Val Val Pro Gln Asn Arg Leu Glu 
    50                  55                  60                  


Met Ala Met Trp Arg Ser Ser Val Thr Glu Leu Asn Leu Ala Leu Ala 
65                  70                  75                  80  


Glu Gly Glu Ala Asn Leu Thr Val Val Val Asp Lys Phe Asp Pro Thr 
                85                  90                  95      


Asp Tyr Arg Gly Gly Val Pro Gly Leu Leu Lys Lys Gly Lys Asp Ile 
            100                 105                 110         


Lys Val Ser Trp Lys Ser Trp Gly His Ser Met Ile Trp Ser Ile Pro 
        115                 120                 125             


Glu Ala Pro Arg Arg Phe Met Val Gly Thr Glu Gly Gln Ser Glu Cys 
    130                 135                 140                 


Pro Leu Glu Arg Arg Lys Thr Gly Val Phe Thr Val Ala Glu Phe Gly 
145                 150                 155                 160 


Val Gly Leu Arg Thr Lys Val Phe Leu Asp Phe Arg Gln Glu Pro Thr 
                165                 170                 175     


His Glu Cys Asp Thr Gly Val Met Gly Ala Ala Val Lys Asn Gly Met 
            180                 185                 190         


Ala Ile His Thr Asp Gln Ser Leu Trp Met Arg Ser Met Lys Asn Asp 
        195                 200                 205             


Thr Gly Thr Tyr Ile Val Glu Leu Leu Val Thr Asp Leu Arg Asn Cys 
    210                 215                 220                 


Ser Trp Pro Ala Ser His Thr Ile Asp Asn Ala Asp Val Val Asp Ser 
225                 230                 235                 240 


Glu Leu Phe Leu Pro Ala Ser Leu Ala Gly Pro Arg Ser Trp Tyr Asn 
                245                 250                 255     


Arg Ile Pro Gly Tyr Ser Glu Gln Val Lys Gly Pro Trp Lys Tyr Thr 
            260                 265                 270         


Pro Ile Arg Val Ile Arg Glu Glu Cys Pro Gly Thr Thr Val Thr Ile 
        275                 280                 285             


Asn Ala Lys Cys Asp Lys Arg Gly Ala Ser Val Arg Ser Thr Thr Glu 
    290                 295                 300                 


Ser Gly Lys Val Ile Pro Glu Trp Cys Cys Arg Ala Cys Thr Met Pro 
305                 310                 315                 320 


Pro Val Thr Phe Arg Thr Gly Thr Asp Cys Trp Tyr Ala Met Glu Ile 
                325                 330                 335     


Arg Pro Val His Asp Gln Gly Gly Leu Val Arg Ser Met Val Val Ala 
            340                 345                 350         


<210>  7
<211>  151
<212>  PRT
<213>  Artificial

<220>
<223>  Dengue virus type 1 NS1 wing domain aa 30-180; X = A or C or S


<220>
<221>  misc_feature
<222>  (150)..(150)
<223>  Xaa can be Ala or Ser or Cys

<400>  7

Glu Gln Tyr Lys Phe Gln Ala Asp Ser Pro Lys Arg Leu Ser Ala Ala 
1               5                   10                  15      


Ile Gly Lys Ala Trp Glu Glu Gly Val Cys Gly Ile Arg Ser Ala Thr 
            20                  25                  30          


Arg Leu Glu Asn Ile Met Trp Lys Gln Ile Ser Asn Glu Leu Asn His 
        35                  40                  45              


Ile Leu Leu Glu Asn Asp Met Lys Phe Thr Val Val Val Gly Asp Val 
    50                  55                  60                  


Ala Gly Ile Leu Ala Gln Gly Lys Lys Met Ile Arg Pro Gln Pro Met 
65                  70                  75                  80  


Glu His Lys Tyr Ser Trp Lys Ser Trp Gly Lys Ala Lys Ile Ile Gly 
                85                  90                  95      


Ala Asp Val Gln Asn Thr Thr Phe Ile Ile Asp Gly Pro Asn Thr Pro 
            100                 105                 110         


Glu Cys Pro Asp Asp Gln Arg Ala Trp Asn Ile Trp Glu Val Glu Asp 
        115                 120                 125             


Tyr Gly Phe Gly Ile Phe Thr Thr Asn Ile Trp Leu Lys Leu Arg Asp 
    130                 135                 140                 


Ser Tyr Thr Gln Val Xaa Asp 
145                 150     


<210>  8
<211>  352
<212>  PRT
<213>  Dengue virus type 1

<400>  8

Asp Ser Gly Cys Val Ile Asn Trp Lys Gly Arg Glu Leu Lys Cys Gly 
1               5                   10                  15      


Ser Gly Ile Phe Val Thr Asn Glu Val His Thr Trp Thr Glu Gln Tyr 
            20                  25                  30          


Lys Phe Gln Ala Asp Ser Pro Lys Arg Leu Ser Ala Ala Ile Gly Lys 
        35                  40                  45              


Ala Trp Glu Glu Gly Val Cys Gly Ile Arg Ser Ala Thr Arg Leu Glu 
    50                  55                  60                  


Asn Ile Met Trp Lys Gln Ile Ser Asn Glu Leu Asn His Ile Leu Leu 
65                  70                  75                  80  


Glu Asn Gly Met Lys Phe Thr Val Val Val Gly Glu Val Asn Gly Ile 
                85                  90                  95      


Leu Ala Gln Gly Lys Lys Met Ile Arg Pro Gln Pro Met Glu His Lys 
            100                 105                 110         


Tyr Ser Trp Lys Ser Trp Gly Lys Ala Lys Val Ile Gly Ala Asp Val 
        115                 120                 125             


Gln Asn Thr Thr Phe Ile Ile Asp Gly Pro Asn Thr Pro Glu Cys Pro 
    130                 135                 140                 


Asp Asp Gln Arg Ala Trp Asn Ile Trp Glu Val Glu Asp Tyr Gly Phe 
145                 150                 155                 160 


Gly Ile Phe Thr Thr Asn Ile Trp Leu Lys Leu Arg Asp Ser Tyr Thr 
                165                 170                 175     


Gln Val Cys Asp His Arg Leu Met Ser Ala Ala Ile Lys Asp Ser Lys 
            180                 185                 190         


Ala Val His Ala Asp Met Gly Tyr Trp Ile Glu Ser Glu Lys Asn Glu 
        195                 200                 205             


Thr Trp Lys Leu Ala Arg Ala Ser Phe Ile Glu Val Lys Thr Cys Ile 
    210                 215                 220                 


Trp Pro Lys Ser His Thr Leu Trp Ser Asn Gly Val Leu Glu Ser Glu 
225                 230                 235                 240 


Met Ile Ile Pro Lys Ile Tyr Gly Gly Pro Ile Ser Gln His Asn Tyr 
                245                 250                 255     


Arg Pro Gly Tyr Phe Thr Gln Thr Ala Gly Pro Trp His Leu Gly Lys 
            260                 265                 270         


Leu Glu Leu Asp Phe Glu Leu Cys Glu Gly Thr Thr Val Val Val Asp 
        275                 280                 285             


Glu His Cys Gly Asn Arg Gly Pro Ser Leu Arg Thr Thr Thr Val Thr 
    290                 295                 300                 


Gly Lys Ile Ile His Glu Trp Cys Cys Arg Ser Cys Thr Leu Pro Pro 
305                 310                 315                 320 


Leu Arg Phe Lys Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg 
                325                 330                 335     


Pro Val Lys Glu Lys Glu Glu Asn Leu Val Lys Ser Met Val Ser Ala 
            340                 345                 350         


<210>  9
<211>  151
<212>  PRT
<213>  Artificial

<220>
<223>  Dengue virus type 2 NS1 wing domain aa 30-180; X = A or C or S


<220>
<221>  misc_feature
<222>  (150)..(150)
<223>  Xaa can be Ala or Ser or Cys

<400>  9

Glu Gln Tyr Lys Phe Gln Pro Glu Ser Pro Ser Lys Leu Ala Ser Ala 
1               5                   10                  15      


Ile Gln Lys Ala His Glu Glu Gly Ile Cys Gly Ile Arg Ser Val Thr 
            20                  25                  30          


Arg Leu Glu Asn Leu Met Trp Lys Gln Ile Thr Pro Glu Leu Asn His 
        35                  40                  45              


Ile Leu Ser Glu Asn Glu Val Lys Leu Thr Ile Met Thr Gly Asp Ile 
    50                  55                  60                  


Lys Gly Ile Met Gln Ala Gly Lys Arg Ser Leu Arg Pro Gln Pro Thr 
65                  70                  75                  80  


Glu Leu Lys Tyr Ser Trp Lys Thr Trp Gly Lys Ala Lys Met Leu Ser 
                85                  90                  95      


Thr Glu Ser His Asn Gln Thr Phe Leu Ile Asp Gly Pro Glu Thr Ala 
            100                 105                 110         


Glu Cys Pro Asn Thr Asn Arg Ala Trp Asn Ser Leu Glu Val Glu Asp 
        115                 120                 125             


Tyr Gly Phe Gly Val Phe Thr Thr Asn Ile Trp Leu Lys Leu Lys Glu 
    130                 135                 140                 


Lys Gln Asp Val Phe Xaa Asp 
145                 150     


<210>  10
<211>  352
<212>  PRT
<213>  Dengue virus type 2

<400>  10

Asp Ser Gly Cys Val Val Ser Trp Lys Asn Lys Glu Leu Lys Cys Gly 
1               5                   10                  15      


Ser Gly Ile Phe Ile Thr Asp Asn Val His Thr Trp Thr Glu Gln Tyr 
            20                  25                  30          


Lys Phe Gln Pro Glu Ser Pro Ser Lys Leu Ala Ser Ala Ile Gln Lys 
        35                  40                  45              


Ala His Glu Glu Gly Ile Cys Gly Ile Arg Ser Val Thr Arg Leu Glu 
    50                  55                  60                  


Asn Leu Met Trp Lys Gln Ile Thr Pro Glu Leu Asn His Ile Leu Ser 
65                  70                  75                  80  


Glu Asn Glu Val Lys Leu Thr Ile Met Thr Gly Asp Ile Lys Gly Ile 
                85                  90                  95      


Met Gln Ala Gly Lys Arg Ser Leu Arg Pro Gln Pro Thr Glu Leu Lys 
            100                 105                 110         


Tyr Ser Trp Lys Thr Trp Gly Lys Ala Lys Met Leu Ser Thr Glu Ser 
        115                 120                 125             


His Asn Gln Thr Phe Leu Ile Asp Gly Pro Glu Thr Ala Glu Cys Pro 
    130                 135                 140                 


Asn Thr Asn Arg Ala Trp Asn Ser Leu Glu Val Glu Asp Tyr Gly Phe 
145                 150                 155                 160 


Gly Val Phe Thr Thr Asn Ile Trp Leu Lys Leu Lys Glu Lys Gln Asp 
                165                 170                 175     


Val Phe Cys Asp Ser Lys Leu Met Ser Ala Ala Ile Lys Asp Asn Arg 
            180                 185                 190         


Ala Val His Ala Asp Met Gly Tyr Trp Ile Glu Ser Ala Leu Asn Asp 
        195                 200                 205             


Thr Trp Lys Ile Glu Lys Ala Ser Phe Ile Glu Val Lys Asn Cys His 
    210                 215                 220                 


Trp Pro Lys Ser His Thr Leu Trp Ser Asn Gly Val Leu Glu Ser Glu 
225                 230                 235                 240 


Met Ile Ile Pro Lys Asn Leu Ala Gly Pro Val Ser Gln His Asn Tyr 
                245                 250                 255     


Arg Pro Gly Tyr His Thr Gln Ile Thr Gly Pro Trp His Leu Gly Lys 
            260                 265                 270         


Leu Glu Met Asp Phe Asp Phe Cys Asp Gly Thr Thr Val Val Val Thr 
        275                 280                 285             


Glu Asp Cys Gly Asn Arg Gly Pro Ser Leu Arg Thr Thr Thr Ala Ser 
    290                 295                 300                 


Gly Lys Leu Ile Thr Glu Trp Cys Cys Arg Ser Cys Thr Leu Pro Pro 
305                 310                 315                 320 


Leu Arg Tyr Arg Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg 
                325                 330                 335     


Pro Leu Lys Glu Lys Glu Glu Asn Leu Val Asn Ser Leu Val Thr Ala 
            340                 345                 350         


<210>  11
<211>  151
<212>  PRT
<213>  Artificial

<220>
<223>  Dengue virus type 3 NS1 wing domain aa 30-180; X = A or C or S


<220>
<221>  misc_feature
<222>  (150)..(150)
<223>  Xaa can be Ala or Ser or Cys

<400>  11

Glu Gln Tyr Lys Phe Gln Ala Asp Ser Pro Lys Arg Leu Ala Thr Ala 
1               5                   10                  15      


Ile Ala Gly Ala Trp Glu Asn Gly Val Cys Gly Ile Arg Ser Thr Thr 
            20                  25                  30          


Arg Met Glu Asn Leu Leu Trp Lys Gln Ile Ala Asn Glu Leu Asn Tyr 
        35                  40                  45              


Ile Leu Trp Glu Asn Asn Ile Lys Leu Thr Val Val Val Gly Asp Ile 
    50                  55                  60                  


Ile Gly Ile Leu Glu Gln Gly Lys Arg Thr Leu Thr Pro Gln Pro Met 
65                  70                  75                  80  


Glu Leu Lys Tyr Ser Trp Lys Thr Trp Gly Lys Ala Lys Ile Val Thr 
                85                  90                  95      


Ala Glu Thr Gln Asn Ser Ser Phe Ile Ile Asp Gly Pro Asn Thr Pro 
            100                 105                 110         


Glu Cys Pro Asn Ala Ser Arg Ala Trp Asn Val Trp Glu Val Glu Asp 
        115                 120                 125             


Tyr Gly Phe Gly Val Phe Thr Thr Asn Ile Trp Leu Lys Leu Arg Glu 
    130                 135                 140                 


Met Tyr Ser Gln Leu Xaa Asp 
145                 150     


<210>  12
<211>  352
<212>  PRT
<213>  Dengue virus type 3

<400>  12

Asp Met Gly Cys Val Ile Asn Trp Lys Gly Lys Glu Leu Lys Cys Gly 
1               5                   10                  15      


Ser Gly Ile Phe Val Thr Asn Glu Val His Thr Trp Thr Glu Gln Tyr 
            20                  25                  30          


Lys Phe Gln Ala Asp Ser Pro Lys Arg Leu Ala Thr Ala Ile Ala Gly 
        35                  40                  45              


Ala Trp Glu Asn Gly Val Cys Gly Ile Arg Ser Thr Thr Arg Met Glu 
    50                  55                  60                  


Asn Leu Leu Trp Arg Gln Ile Ala Asn Glu Leu Asn Tyr Ile Leu Trp 
65                  70                  75                  80  


Glu Asn Asn Ile Lys Leu Thr Val Val Val Gly Asp Ile Ile Gly Ile 
                85                  90                  95      


Leu Glu Gln Gly Lys Arg Thr Leu Thr Pro Gln Pro Met Glu Leu Lys 
            100                 105                 110         


Tyr Ser Trp Lys Thr Trp Gly Lys Ala Lys Ile Val Thr Ala Glu Thr 
        115                 120                 125             


Gln Asn Ser Ser Phe Ile Ile Asp Gly Pro Asn Thr Pro Glu Cys Pro 
    130                 135                 140                 


Asn Ala Ser Arg Ala Trp Asn Val Trp Glu Val Glu Asp Tyr Gly Phe 
145                 150                 155                 160 


Gly Val Phe Thr Thr Asn Ile Trp Leu Lys Leu Arg Glu Met Tyr Ser 
                165                 170                 175     


Gln Leu Cys Asp His Arg Leu Met Ser Ala Ala Val Lys Asp Glu Arg 
            180                 185                 190         


Ala Val His Ala Asp Met Gly Tyr Trp Ile Glu Ser Gln Lys Asn Gly 
        195                 200                 205             


Ser Trp Lys Leu Glu Lys Ala Ser Leu Ile Glu Val Lys Thr Cys Thr 
    210                 215                 220                 


Trp Pro Lys Ser His Thr Leu Trp Ser Asn Gly Val Leu Glu Ser Asp 
225                 230                 235                 240 


Met Ile Ile Pro Lys Ser Leu Ala Gly Pro Ile Ser Gln His Asn Tyr 
                245                 250                 255     


Arg Pro Gly Tyr His Thr Gln Thr Ala Gly Pro Trp His Leu Gly Lys 
            260                 265                 270         


Leu Glu Leu Asp Phe Asn Tyr Cys Glu Gly Thr Thr Val Val Ile Thr 
        275                 280                 285             


Glu Asn Cys Gly Thr Arg Gly Pro Ser Leu Arg Thr Thr Thr Val Ser 
    290                 295                 300                 


Gly Lys Leu Ile His Glu Trp Cys Cys Arg Ser Cys Thr Leu Pro Pro 
305                 310                 315                 320 


Leu Arg Tyr Met Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg 
                325                 330                 335     


Pro Ile Asn Glu Lys Glu Glu Asn Met Val Lys Ser Leu Val Ser Ala 
            340                 345                 350         


<210>  13
<211>  151
<212>  PRT
<213>  Artificial

<220>
<223>  Dengue virus type 4 NS1 wing domain aa 30-180; X = A or C or S


<220>
<221>  misc_feature
<222>  (150)..(150)
<223>  Xaa can be Ala or Ser or Cys

<400>  13

Glu Gln Tyr Lys Phe Gln Pro Glu Ser Pro Ala Arg Leu Ala Ser Ala 
1               5                   10                  15      


Ile Leu Asn Ala His Lys Asp Gly Val Cys Gly Ile Arg Ser Thr Thr 
            20                  25                  30          


Arg Leu Glu Asn Ile Met Trp Lys Gln Ile Thr Asn Glu Leu Asn Tyr 
        35                  40                  45              


Val Leu Trp Glu Gly Gly His Asp Leu Thr Val Val Ala Gly Asp Val 
    50                  55                  60                  


Lys Gly Val Leu Thr Lys Gly Lys Arg Ala Leu Thr Pro Pro Val Asn 
65                  70                  75                  80  


Asp Leu Lys Tyr Ser Trp Lys Thr Trp Gly Lys Ala Lys Ile Phe Thr 
                85                  90                  95      


Pro Glu Ala Arg Asn Ser Thr Phe Leu Ile Asp Gly Pro Asp Thr Ser 
            100                 105                 110         


Glu Cys Pro Asn Glu Arg Arg Ala Trp Asn Phe Phe Glu Val Glu Asp 
        115                 120                 125             


Tyr Gly Phe Gly Met Phe Thr Thr Asn Ile Trp Met Lys Phe Arg Glu 
    130                 135                 140                 


Gly Ser Ser Glu Val Xaa Asp 
145                 150     


<210>  14
<211>  352
<212>  PRT
<213>  Dengue virus type 4

<400>  14

Asp Thr Gly Cys Ala Val Ser Trp Ser Gly Lys Glu Leu Lys Cys Gly 
1               5                   10                  15      


Ser Gly Ile Phe Val Ile Asp Asn Val His Thr Trp Thr Glu Gln Tyr 
            20                  25                  30          


Lys Phe Gln Pro Glu Ser Pro Ala Arg Leu Ala Ser Ala Ile Leu Asn 
        35                  40                  45              


Ala His Glu Asp Gly Val Cys Gly Ile Arg Ser Thr Thr Arg Leu Glu 
    50                  55                  60                  


Asn Ile Met Trp Lys Gln Ile Thr Asn Glu Leu Asn Tyr Val Leu Trp 
65                  70                  75                  80  


Glu Gly Gly His Asp Leu Thr Val Val Ala Gly Asp Val Lys Gly Val 
                85                  90                  95      


Leu Ser Lys Gly Lys Arg Ala Leu Ala Pro Pro Val Asn Asp Leu Lys 
            100                 105                 110         


Tyr Ser Trp Lys Thr Trp Gly Lys Ala Lys Ile Phe Thr Pro Glu Ala 
        115                 120                 125             


Lys Asn Ser Thr Phe Leu Ile Asp Gly Pro Asp Thr Ser Glu Cys Pro 
    130                 135                 140                 


Asn Glu Arg Arg Ala Trp Asn Phe Leu Glu Val Glu Asp Tyr Gly Phe 
145                 150                 155                 160 


Gly Met Phe Thr Thr Asn Ile Trp Met Lys Phe Arg Glu Gly Ser Ser 
                165                 170                 175     


Glu Val Cys Asp His Arg Leu Met Ser Ala Ala Ile Lys Asp Gln Lys 
            180                 185                 190         


Ala Val His Ala Asp Met Gly Tyr Trp Ile Glu Ser Ser Lys Asn Gln 
        195                 200                 205             


Thr Trp Gln Ile Glu Lys Ala Ser Leu Ile Glu Val Lys Thr Cys Leu 
    210                 215                 220                 


Trp Pro Lys Thr His Thr Leu Trp Ser Asn Gly Val Leu Glu Ser Gln 
225                 230                 235                 240 


Met Leu Ile Pro Lys Ala Tyr Ala Gly Pro Phe Ser Gln His Asn Tyr 
                245                 250                 255     


Arg Gln Gly Tyr Ala Thr Gln Thr Val Gly Pro Trp His Leu Gly Lys 
            260                 265                 270         


Leu Glu Ile Asp Phe Gly Glu Cys Pro Gly Thr Thr Val Thr Ile Gln 
        275                 280                 285             


Glu Asp Cys Asp His Arg Gly Pro Ser Leu Arg Thr Thr Thr Ala Ser 
    290                 295                 300                 


Gly Lys Leu Val Thr Gln Trp Cys Cys Arg Ser Cys Thr Met Pro Pro 
305                 310                 315                 320 


Leu Arg Phe Leu Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg 
                325                 330                 335     


Pro Leu Ser Glu Lys Glu Glu Asn Met Val Lys Ser Gln Val Ser Ala 
            340                 345                 350         


<210>  15
<211>  151
<212>  PRT
<213>  Artificial

<220>
<223>  West-Nile virus NS1 wing domain aa 30-180; X = A or C or S


<220>
<221>  misc_feature
<222>  (150)..(150)
<223>  Xaa can be Ala or Ser or Cys

<400>  15

Asp Arg Tyr Lys Phe Tyr Pro Glu Thr Pro Gln Gly Leu Ala Lys Ile 
1               5                   10                  15      


Ile Gln Lys Ala His Ala Glu Gly Val Cys Gly Leu Arg Ser Val Ser 
            20                  25                  30          


Arg Leu Glu His Gln Met Trp Glu Ala Ile Lys Asp Glu Leu Asn Thr 
        35                  40                  45              


Leu Leu Lys Glu Asn Gly Val Asp Leu Ser Val Val Val Glu Lys Gln 
    50                  55                  60                  


Asn Gly Met Tyr Lys Ala Ala Pro Lys Arg Leu Ala Ala Thr Thr Glu 
65                  70                  75                  80  


Lys Leu Glu Met Gly Trp Lys Ala Trp Gly Lys Ser Ile Ile Phe Ala 
                85                  90                  95      


Pro Glu Leu Ala Asn Asn Thr Phe Val Ile Asp Gly Pro Glu Thr Glu 
            100                 105                 110         


Glu Cys Pro Thr Ala Asn Arg Ala Trp Asn Ser Met Glu Val Glu Asp 
        115                 120                 125             


Phe Gly Phe Gly Leu Thr Ser Thr Arg Met Phe Leu Arg Ile Arg Glu 
    130                 135                 140                 


Thr Asn Thr Thr Glu Xaa Asp 
145                 150     


<210>  16
<211>  352
<212>  PRT
<213>  West Nile virus

<400>  16

Asp Thr Gly Cys Ala Ile Asp Ile Gly Arg Gln Glu Leu Arg Cys Gly 
1               5                   10                  15      


Ser Gly Val Phe Ile His Asn Asp Val Glu Ala Trp Met Asp Arg Tyr 
            20                  25                  30          


Lys Phe Tyr Pro Glu Thr Pro Gln Gly Leu Ala Lys Ile Ile Gln Lys 
        35                  40                  45              


Ala His Ala Glu Gly Val Cys Gly Leu Arg Ser Val Ser Arg Leu Glu 
    50                  55                  60                  


His Gln Met Trp Glu Ala Ile Lys Asp Glu Leu Asn Thr Leu Leu Lys 
65                  70                  75                  80  


Glu Asn Gly Val Asp Leu Ser Val Val Val Glu Lys Gln Asn Gly Met 
                85                  90                  95      


Tyr Lys Ala Ala Pro Lys Arg Leu Ala Ala Thr Thr Glu Lys Leu Glu 
            100                 105                 110         


Met Gly Trp Lys Ala Trp Gly Lys Ser Ile Ile Phe Ala Pro Glu Leu 
        115                 120                 125             


Ala Asn Asn Thr Phe Val Ile Asp Gly Pro Glu Thr Glu Glu Cys Pro 
    130                 135                 140                 


Thr Ala Asn Arg Ala Trp Asn Ser Met Glu Val Glu Asp Phe Gly Phe 
145                 150                 155                 160 


Gly Leu Thr Ser Thr Arg Met Phe Leu Arg Ile Arg Glu Thr Asn Thr 
                165                 170                 175     


Thr Glu Cys Asp Ser Lys Ile Ile Gly Thr Ala Val Lys Asn Asn Met 
            180                 185                 190         


Ala Val His Ser Asp Leu Ser Tyr Trp Ile Glu Ser Gly Leu Asn Asp 
        195                 200                 205             


Thr Trp Lys Leu Glu Arg Ala Val Leu Gly Glu Val Lys Ser Cys Thr 
    210                 215                 220                 


Trp Pro Glu Thr His Thr Leu Trp Gly Asp Gly Val Leu Glu Ser Asp 
225                 230                 235                 240 


Leu Ile Ile Pro Ile Thr Leu Ala Gly Pro Arg Ser Asn His Asn Arg 
                245                 250                 255     


Arg Pro Gly Tyr Lys Thr Gln Asn Gln Gly Pro Trp Asp Glu Gly Arg 
            260                 265                 270         


Val Glu Ile Asp Phe Asp Tyr Cys Pro Gly Thr Thr Val Thr Ile Ser 
        275                 280                 285             


Asp Ser Cys Glu His Arg Gly Pro Ala Ala Arg Thr Thr Thr Glu Ser 
    290                 295                 300                 


Gly Lys Leu Ile Thr Asp Trp Cys Cys Arg Ser Cys Thr Leu Pro Pro 
305                 310                 315                 320 


Leu Arg Phe Gln Thr Glu Asn Gly Cys Trp Tyr Gly Met Glu Ile Arg 
                325                 330                 335     


Pro Thr Arg His Asp Glu Lys Thr Leu Val Gln Ser Arg Val Asn Ala 
            340                 345                 350         


<210>  17
<211>  151
<212>  PRT
<213>  Artificial

<220>
<223>  Yellow fever virus NS1 wing domain aa 30-180; X = A or C or S


<220>
<221>  misc_feature
<222>  (150)..(150)
<223>  Xaa can be Ala or Ser or Cys

<400>  17

Asn Lys Tyr Ser Tyr Tyr Pro Glu Asp Pro Val Lys Leu Ala Ser Ile 
1               5                   10                  15      


Val Lys Ala Ser Phe Glu Glu Gly Lys Cys Gly Leu Asn Ser Val Asp 
            20                  25                  30          


Ser Leu Glu His Glu Met Trp Arg Ser Arg Ala Asp Glu Ile Asn Ala 
        35                  40                  45              


Ile Phe Glu Glu Asn Glu Val Asp Ile Ser Val Val Val Gln Asp Pro 
    50                  55                  60                  


Lys Asn Val Tyr Gln Arg Gly Thr His Pro Phe Ser Arg Ile Arg Asp 
65                  70                  75                  80  


Gly Leu Gln Tyr Gly Trp Lys Thr Trp Gly Lys Asn Leu Val Phe Ser 
                85                  90                  95      


Pro Gly Arg Lys Asn Gly Ser Phe Ile Ile Asp Gly Lys Ser Arg Lys 
            100                 105                 110         


Glu Cys Pro Phe Ser Asn Arg Val Trp Asn Ser Phe Gln Ile Glu Glu 
        115                 120                 125             


Phe Gly Thr Gly Val Phe Thr Thr Arg Val Tyr Met Asp Ala Val Phe 
    130                 135                 140                 


Glu Tyr Thr Ile Asp Xaa Asp 
145                 150     


<210>  18
<211>  352
<212>  PRT
<213>  Yellow fever virus

<400>  18

Asp Gln Gly Cys Ala Ile Asn Phe Gly Lys Arg Glu Leu Lys Cys Gly 
1               5                   10                  15      


Asp Gly Ile Phe Ile Phe Arg Asp Ser Asp Asp Trp Leu Asn Lys Tyr 
            20                  25                  30          


Ser Tyr Tyr Pro Glu Asp Pro Val Lys Leu Ala Ser Ile Val Lys Ala 
        35                  40                  45              


Ser Phe Glu Glu Gly Lys Cys Gly Leu Asn Ser Val Asp Ser Leu Glu 
    50                  55                  60                  


His Glu Met Trp Arg Ser Arg Ala Asp Glu Ile Asn Ala Ile Phe Glu 
65                  70                  75                  80  


Glu Asn Glu Val Asp Ile Ser Val Val Val Gln Asp Pro Lys Asn Val 
                85                  90                  95      


Tyr Gln Arg Gly Thr His Pro Phe Ser Arg Ile Arg Asp Gly Leu Gln 
            100                 105                 110         


Tyr Gly Trp Lys Thr Trp Gly Lys Asn Leu Val Phe Ser Pro Gly Arg 
        115                 120                 125             


Lys Asn Gly Ser Phe Ile Ile Asp Gly Lys Ser Arg Lys Glu Cys Pro 
    130                 135                 140                 


Phe Ser Asn Arg Val Trp Asn Ser Phe Gln Ile Glu Glu Phe Gly Thr 
145                 150                 155                 160 


Gly Val Phe Thr Thr Arg Val Tyr Met Asp Ala Val Phe Glu Tyr Thr 
                165                 170                 175     


Ile Asp Cys Asp Gly Ser Ile Leu Gly Ala Ala Val Asn Gly Lys Lys 
            180                 185                 190         


Ser Ala His Gly Ser Pro Thr Phe Trp Met Gly Ser His Glu Val Asn 
        195                 200                 205             


Gly Thr Trp Met Ile His Thr Leu Glu Ala Leu Asp Tyr Lys Glu Cys 
    210                 215                 220                 


Glu Trp Pro Leu Thr His Thr Ile Gly Thr Ser Val Glu Glu Ser Glu 
225                 230                 235                 240 


Met Phe Met Pro Arg Ser Ile Gly Gly Pro Val Ser Ser His Asn His 
                245                 250                 255     


Ile Pro Gly Tyr Lys Val Gln Thr Asn Gly Pro Trp Met Gln Val Pro 
            260                 265                 270         


Leu Glu Val Lys Arg Glu Ala Cys Pro Gly Thr Ser Val Ile Ile Asp 
        275                 280                 285             


Gly Asn Cys Asp Gly Arg Gly Lys Ser Thr Arg Ser Thr Thr Asp Ser 
    290                 295                 300                 


Gly Lys Val Ile Pro Glu Trp Cys Cys Arg Ser Cys Thr Met Pro Pro 
305                 310                 315                 320 


Val Ser Phe His Gly Ser Asp Gly Cys Trp Tyr Pro Met Glu Ile Arg 
                325                 330                 335     


Pro Arg Lys Thr His Glu Ser His Leu Val Arg Ser Trp Val Thr Ala 
            340                 345                 350         


<210>  19
<211>  151
<212>  PRT
<213>  Artificial

<220>
<223>  Japanese encephalitis virus NS1 wing domain aa 30-180; X = A or C
       or S


<220>
<221>  misc_feature
<222>  (150)..(150)
<223>  Xaa can be Ala or Ser or Cys

<400>  19

Asp Arg Tyr Lys Tyr Leu Pro Glu Thr Pro Arg Ser Leu Ala Lys Ile 
1               5                   10                  15      


Val His Lys Ala His Lys Glu Gly Val Cys Gly Val Arg Ser Val Thr 
            20                  25                  30          


Arg Leu Glu His Gln Met Trp Glu Ala Val Arg Asp Glu Leu Asn Val 
        35                  40                  45              


Leu Leu Lys Glu Asn Ala Val Asp Leu Ser Val Val Val Asn Lys Pro 
    50                  55                  60                  


Val Gly Arg Tyr Arg Ser Ala Pro Lys Arg Leu Ser Met Thr Gln Glu 
65                  70                  75                  80  


Lys Phe Glu Met Gly Trp Lys Ala Trp Gly Lys Ser Ile Leu Phe Ala 
                85                  90                  95      


Pro Glu Leu Ala Asn Ser Thr Phe Val Val Asp Gly Pro Glu Thr Lys 
            100                 105                 110         


Glu Cys Pro Asp Glu His Arg Ala Trp Asn Ser Met Gln Ile Glu Asp 
        115                 120                 125             


Phe Gly Phe Gly Ile Thr Ser Thr Arg Val Trp Leu Lys Ile Arg Glu 
    130                 135                 140                 


Glu Ser Thr Asp Glu Xaa Asp 
145                 150     


<210>  20
<211>  352
<212>  PRT
<213>  Japanese encephalitis virus

<400>  20

Asp Thr Gly Cys Ala Ile Asp Ile Thr Arg Lys Glu Met Arg Cys Gly 
1               5                   10                  15      


Ser Gly Ile Phe Val His Asn Asp Val Glu Ala Trp Val Asp Arg Tyr 
            20                  25                  30          


Lys Tyr Leu Pro Glu Thr Pro Arg Ser Leu Ala Lys Ile Val His Lys 
        35                  40                  45              


Ala His Lys Glu Gly Val Cys Gly Val Arg Ser Val Thr Arg Leu Glu 
    50                  55                  60                  


His Gln Met Trp Glu Ala Val Arg Asp Glu Leu Asn Val Leu Leu Lys 
65                  70                  75                  80  


Glu Asn Ala Val Asp Leu Ser Val Val Val Asn Lys Pro Val Gly Arg 
                85                  90                  95      


Tyr Arg Ser Ala Pro Lys Arg Leu Ser Met Thr Gln Glu Lys Phe Glu 
            100                 105                 110         


Met Gly Trp Lys Ala Trp Gly Lys Ser Ile Leu Phe Ala Pro Glu Leu 
        115                 120                 125             


Ala Asn Ser Thr Phe Val Val Asp Gly Pro Glu Thr Lys Glu Cys Pro 
    130                 135                 140                 


Asp Glu His Arg Ala Trp Asn Ser Met Gln Ile Glu Asp Phe Gly Phe 
145                 150                 155                 160 


Gly Ile Thr Ser Thr Arg Val Trp Leu Lys Ile Arg Glu Glu Ser Thr 
                165                 170                 175     


Asp Glu Cys Asp Gly Ala Ile Ile Gly Thr Ala Val Lys Gly His Val 
            180                 185                 190         


Ala Val His Ser Asp Leu Ser Tyr Trp Ile Glu Ser Arg Tyr Asn Asp 
        195                 200                 205             


Thr Trp Lys Leu Glu Arg Ala Val Phe Gly Glu Val Lys Ser Cys Thr 
    210                 215                 220                 


Trp Pro Glu Thr His Thr Leu Trp Gly Asp Gly Val Glu Glu Ser Glu 
225                 230                 235                 240 


Leu Ile Ile Pro His Thr Ile Ala Gly Pro Lys Ser Lys His Asn Arg 
                245                 250                 255     


Arg Glu Gly Tyr Lys Thr Gln Asn Gln Gly Pro Trp Asp Glu Asn Gly 
            260                 265                 270         


Ile Val Leu Asp Phe Asp Tyr Cys Pro Gly Thr Lys Val Thr Ile Thr 
        275                 280                 285             


Glu Asp Cys Gly Lys Arg Gly Pro Ser Val Arg Thr Thr Thr Asp Ser 
    290                 295                 300                 


Gly Lys Leu Ile Thr Asp Trp Cys Cys Arg Ser Cys Ser Leu Pro Pro 
305                 310                 315                 320 


Leu Arg Phe Arg Thr Glu Asn Gly Cys Trp Tyr Gly Met Glu Ile Arg 
                325                 330                 335     


Pro Val Arg His Asp Glu Ala Thr Leu Val Arg Ser Gln Val Asp Ala 
            340                 345                 350         


<210>  21
<211>  534
<212>  PRT
<213>  Artificial

<220>
<223>  Fusion protein of tandem E. coli SlyD with Zika virus NS1 wing 
       domain aa 30-180

<400>  21

Met Lys Val Ala Lys Asp Leu Val Val Ser Leu Ala Tyr Gln Val Arg 
1               5                   10                  15      


Thr Glu Asp Gly Val Leu Val Asp Glu Ser Pro Val Ser Ala Pro Leu 
            20                  25                  30          


Asp Tyr Leu His Gly His Gly Ser Leu Ile Ser Gly Leu Glu Thr Ala 
        35                  40                  45              


Leu Glu Gly His Glu Val Gly Asp Lys Phe Asp Val Ala Val Gly Ala 
    50                  55                  60                  


Asn Asp Ala Tyr Gly Gln Tyr Asp Glu Asn Leu Val Gln Arg Val Pro 
65                  70                  75                  80  


Lys Asp Val Phe Met Gly Val Asp Glu Leu Gln Val Gly Met Arg Phe 
                85                  90                  95      


Leu Ala Glu Thr Asp Gln Gly Pro Val Pro Val Glu Ile Thr Ala Val 
            100                 105                 110         


Glu Asp Asp His Val Val Val Asp Gly Asn His Met Leu Ala Gly Gln 
        115                 120                 125             


Asn Leu Lys Phe Asn Val Glu Val Val Ala Ile Arg Glu Ala Thr Glu 
    130                 135                 140                 


Glu Glu Leu Ala His Gly His Val His Gly Ala His Asp His His His 
145                 150                 155                 160 


Asp His Asp His Asp Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly 
                165                 170                 175     


Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Lys Val Ala Lys 
            180                 185                 190         


Asp Leu Val Val Ser Leu Ala Tyr Gln Val Arg Thr Glu Asp Gly Val 
        195                 200                 205             


Leu Val Asp Glu Ser Pro Val Ser Ala Pro Leu Asp Tyr Leu His Gly 
    210                 215                 220                 


His Gly Ser Leu Ile Ser Gly Leu Glu Thr Ala Leu Glu Gly His Glu 
225                 230                 235                 240 


Val Gly Asp Lys Phe Asp Val Ala Val Gly Ala Asn Asp Ala Tyr Gly 
                245                 250                 255     


Gln Tyr Asp Glu Asn Leu Val Gln Arg Val Pro Lys Asp Val Phe Met 
            260                 265                 270         


Gly Val Asp Glu Leu Gln Val Gly Met Arg Phe Leu Ala Glu Thr Asp 
        275                 280                 285             


Gln Gly Pro Val Pro Val Glu Ile Thr Ala Val Glu Asp Asp His Val 
    290                 295                 300                 


Val Val Asp Gly Asn His Met Leu Ala Gly Gln Asn Leu Lys Phe Asn 
305                 310                 315                 320 


Val Glu Val Val Ala Ile Arg Glu Ala Thr Glu Glu Glu Leu Ala His 
                325                 330                 335     


Gly His Val His Gly Ala His Asp His His His Asp His Asp His Asp 
            340                 345                 350         


Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
        355                 360                 365             


Gly Gly Gly Ser Gly Gly Gly Asp Arg Tyr Lys Tyr His Pro Asp Ser 
    370                 375                 380                 


Pro Arg Arg Leu Ala Ala Ala Val Lys Gln Ala Trp Glu Glu Gly Ile 
385                 390                 395                 400 


Cys Gly Ile Ser Ser Val Ser Arg Met Glu Asn Ile Met Trp Lys Ser 
                405                 410                 415     


Val Glu Gly Glu Leu Asn Ala Ile Leu Glu Glu Asn Gly Val Gln Leu 
            420                 425                 430         


Thr Val Val Val Gly Ser Val Lys Asn Pro Met Trp Arg Gly Pro Gln 
        435                 440                 445             


Arg Leu Pro Val Pro Val Asn Glu Leu Pro His Gly Trp Lys Ala Trp 
    450                 455                 460                 


Gly Lys Ser Tyr Phe Val Arg Ala Ala Lys Thr Asn Asn Ser Phe Val 
465                 470                 475                 480 


Val Asp Gly Asp Thr Leu Lys Glu Cys Pro Leu Lys His Arg Ala Trp 
                485                 490                 495     


Asn Ser Phe Leu Val Glu Asp His Gly Phe Gly Val Phe His Thr Ser 
            500                 505                 510         


Val Trp Leu Lys Val Arg Glu Asp Tyr Ser Leu Glu Ala Asp Leu Glu 
        515                 520                 525             


His His His His His His 
    530                 


<210>  22
<211>  545
<212>  PRT
<213>  Artificial

<220>
<223>  Fusion protein of tandem E. coli SlyD with Zika virus NS1 
       -ladder domain (aa 191-352, strain Mr 766)

<400>  22

Met Lys Val Ala Lys Asp Leu Val Val Ser Leu Ala Tyr Gln Val Arg 
1               5                   10                  15      


Thr Glu Asp Gly Val Leu Val Asp Glu Ser Pro Val Ser Ala Pro Leu 
            20                  25                  30          


Asp Tyr Leu His Gly His Gly Ser Leu Ile Ser Gly Leu Glu Thr Ala 
        35                  40                  45              


Leu Glu Gly His Glu Val Gly Asp Lys Phe Asp Val Ala Val Gly Ala 
    50                  55                  60                  


Asn Asp Ala Tyr Gly Gln Tyr Asp Glu Asn Leu Val Gln Arg Val Pro 
65                  70                  75                  80  


Lys Asp Val Phe Met Gly Val Asp Glu Leu Gln Val Gly Met Arg Phe 
                85                  90                  95      


Leu Ala Glu Thr Asp Gln Gly Pro Val Pro Val Glu Ile Thr Ala Val 
            100                 105                 110         


Glu Asp Asp His Val Val Val Asp Gly Asn His Met Leu Ala Gly Gln 
        115                 120                 125             


Asn Leu Lys Phe Asn Val Glu Val Val Ala Ile Arg Glu Ala Thr Glu 
    130                 135                 140                 


Glu Glu Leu Ala His Gly His Val His Gly Ala His Asp His His His 
145                 150                 155                 160 


Asp His Asp His Asp Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly 
                165                 170                 175     


Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Lys Val Ala Lys 
            180                 185                 190         


Asp Leu Val Val Ser Leu Ala Tyr Gln Val Arg Thr Glu Asp Gly Val 
        195                 200                 205             


Leu Val Asp Glu Ser Pro Val Ser Ala Pro Leu Asp Tyr Leu His Gly 
    210                 215                 220                 


His Gly Ser Leu Ile Ser Gly Leu Glu Thr Ala Leu Glu Gly His Glu 
225                 230                 235                 240 


Val Gly Asp Lys Phe Asp Val Ala Val Gly Ala Asn Asp Ala Tyr Gly 
                245                 250                 255     


Gln Tyr Asp Glu Asn Leu Val Gln Arg Val Pro Lys Asp Val Phe Met 
            260                 265                 270         


Gly Val Asp Glu Leu Gln Val Gly Met Arg Phe Leu Ala Glu Thr Asp 
        275                 280                 285             


Gln Gly Pro Val Pro Val Glu Ile Thr Ala Val Glu Asp Asp His Val 
    290                 295                 300                 


Val Val Asp Gly Asn His Met Leu Ala Gly Gln Asn Leu Lys Phe Asn 
305                 310                 315                 320 


Val Glu Val Val Ala Ile Arg Glu Ala Thr Glu Glu Glu Leu Ala His 
                325                 330                 335     


Gly His Val His Gly Ala His Asp His His His Asp His Asp His Asp 
            340                 345                 350         


Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
        355                 360                 365             


Gly Gly Gly Ser Gly Gly Gly Arg Glu Ala Ala His Ser Asp Leu Gly 
    370                 375                 380                 


Tyr Trp Ile Glu Ser Glu Lys Asn Asp Thr Trp Arg Leu Lys Arg Ala 
385                 390                 395                 400 


His Leu Ile Glu Met Lys Thr Ala Glu Trp Pro Lys Ser His Thr Leu 
                405                 410                 415     


Trp Thr Asp Gly Val Glu Glu Ser Asp Leu Ile Ile Pro Lys Ser Leu 
            420                 425                 430         


Ala Gly Pro Leu Ser His His Asn Thr Arg Glu Gly Tyr Arg Thr Gln 
        435                 440                 445             


Val Lys Gly Pro Trp His Ser Glu Glu Leu Glu Ile Arg Phe Glu Glu 
    450                 455                 460                 


Cys Pro Gly Thr Lys Val Tyr Val Glu Glu Thr Cys Gly Thr Arg Gly 
465                 470                 475                 480 


Pro Ser Leu Arg Ser Thr Thr Ala Ser Gly Arg Val Ile Glu Glu Trp 
                485                 490                 495     


Cys Cys Arg Glu Cys Thr Met Pro Pro Leu Ser Phe Arg Ala Lys Asp 
            500                 505                 510         


Gly Cys Trp Tyr Gly Met Glu Ile Arg Pro Arg Lys Glu Pro Glu Ser 
        515                 520                 525             


Asn Leu Val Arg Ser Met Val Thr Ala Leu Glu His His His His His 
    530                 535                 540                 


His 
545 


<210>  23
<211>  358
<212>  PRT
<213>  Artificial

<220>
<223>  Fusion protein of E. coli SlyD with Zika virus NS1 -ladder 
       domain (aa 191-352, strain Mr 766)

<400>  23

Met Lys Val Ala Lys Asp Leu Val Val Ser Leu Ala Tyr Gln Val Arg 
1               5                   10                  15      


Thr Glu Asp Gly Val Leu Val Asp Glu Ser Pro Val Ser Ala Pro Leu 
            20                  25                  30          


Asp Tyr Leu His Gly His Gly Ser Leu Ile Ser Gly Leu Glu Thr Ala 
        35                  40                  45              


Leu Glu Gly His Glu Val Gly Asp Lys Phe Asp Val Ala Val Gly Ala 
    50                  55                  60                  


Asn Asp Ala Tyr Gly Gln Tyr Asp Glu Asn Leu Val Gln Arg Val Pro 
65                  70                  75                  80  


Lys Asp Val Phe Met Gly Val Asp Glu Leu Gln Val Gly Met Arg Phe 
                85                  90                  95      


Leu Ala Glu Thr Asp Gln Gly Pro Val Pro Val Glu Ile Thr Ala Val 
            100                 105                 110         


Glu Asp Asp His Val Val Val Asp Gly Asn His Met Leu Ala Gly Gln 
        115                 120                 125             


Asn Leu Lys Phe Asn Val Glu Val Val Ala Ile Arg Glu Ala Thr Glu 
    130                 135                 140                 


Glu Glu Leu Ala His Gly His Val His Gly Ala His Asp His His His 
145                 150                 155                 160 


Asp His Asp His Asp Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly 
                165                 170                 175     


Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Arg Glu Ala Ala 
            180                 185                 190         


His Ser Asp Leu Gly Tyr Trp Ile Glu Ser Glu Lys Asn Asp Thr Trp 
        195                 200                 205             


Arg Leu Lys Arg Ala His Leu Ile Glu Met Lys Thr Ala Glu Trp Pro 
    210                 215                 220                 


Lys Ser His Thr Leu Trp Thr Asp Gly Val Glu Glu Ser Asp Leu Ile 
225                 230                 235                 240 


Ile Pro Lys Ser Leu Ala Gly Pro Leu Ser His His Asn Thr Arg Glu 
                245                 250                 255     


Gly Tyr Arg Thr Gln Val Lys Gly Pro Trp His Ser Glu Glu Leu Glu 
            260                 265                 270         


Ile Arg Phe Glu Glu Cys Pro Gly Thr Lys Val Tyr Val Glu Glu Thr 
        275                 280                 285             


Cys Gly Thr Arg Gly Pro Ser Leu Arg Ser Thr Thr Ala Ser Gly Arg 
    290                 295                 300                 


Val Ile Glu Glu Trp Cys Cys Arg Glu Cys Thr Met Pro Pro Leu Ser 
305                 310                 315                 320 


Phe Arg Ala Lys Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg Pro Arg 
                325                 330                 335     


Lys Glu Pro Glu Ser Asn Leu Val Arg Ser Met Val Thr Ala Leu Glu 
            340                 345                 350         


His His His His His His 
        355             


<210>  24
<211>  162
<212>  PRT
<213>  Dengue virus type 1

<400>  24

Ser Lys Ala Val His Ala Asp Met Gly Tyr Trp Ile Glu Ser Glu Lys 
1               5                   10                  15      


Asn Glu Thr Trp Lys Leu Ala Arg Ala Ser Phe Ile Glu Val Lys Thr 
            20                  25                  30          


Ala Ile Trp Pro Lys Ser His Thr Leu Trp Ser Asn Gly Val Leu Glu 
        35                  40                  45              


Ser Glu Met Ile Ile Pro Lys Ile Tyr Gly Gly Pro Ile Ser Gln His 
    50                  55                  60                  


Asn Tyr Arg Pro Gly Tyr Phe Thr Gln Thr Ala Gly Pro Trp His Leu 
65                  70                  75                  80  


Gly Lys Leu Glu Leu Asp Phe Asp Leu Cys Glu Gly Thr Thr Val Val 
                85                  90                  95      


Val Asp Glu His Cys Gly Asn Arg Gly Pro Ser Leu Arg Thr Thr Ser 
            100                 105                 110         


Val Thr Gly Lys Ile Ile His Glu Trp Cys Cys Arg Ser Cys Thr Leu 
        115                 120                 125             


Pro Pro Leu Arg Phe Arg Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu 
    130                 135                 140                 


Ile Arg Pro Val Lys Glu Lys Glu Glu Asn Leu Val Lys Ser Met Val 
145                 150                 155                 160 


Ser Ala 
        


<210>  25
<211>  162
<212>  PRT
<213>  Dengue virus type 2

<400>  25

Asn Arg Ala Val His Ala Asp Met Gly Tyr Trp Ile Glu Ser Ala Leu 
1               5                   10                  15      


Asn Asp Thr Trp Lys Ile Glu Lys Ala Ser Phe Ile Glu Val Lys Asn 
            20                  25                  30          


Ala His Trp Pro Lys Ser His Thr Leu Trp Ser Asn Gly Val Leu Glu 
        35                  40                  45              


Ser Glu Met Ile Ile Pro Lys Asn Leu Ala Gly Pro Val Ser Gln His 
    50                  55                  60                  


Asn Tyr Arg Pro Gly Tyr His Thr Gln Ile Ala Gly Pro Trp His Leu 
65                  70                  75                  80  


Gly Lys Leu Glu Met Asp Phe Asp Phe Cys Asp Gly Thr Thr Val Val 
                85                  90                  95      


Val Thr Glu Asp Cys Gly Asn Arg Gly Pro Ser Leu Arg Thr Thr Thr 
            100                 105                 110         


Ala Ser Gly Lys Leu Ile Thr Glu Trp Cys Cys Arg Ser Cys Thr Leu 
        115                 120                 125             


Pro Pro Leu Arg Tyr Arg Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu 
    130                 135                 140                 


Ile Arg Pro Leu Lys Glu Lys Glu Glu Asn Leu Val Asn Ser Leu Val 
145                 150                 155                 160 


Thr Ala 
        


<210>  26
<211>  162
<212>  PRT
<213>  Dengue virus type 3

<400>  26

Glu Arg Ala Val His Ala Asp Met Gly Tyr Trp Ile Glu Ser Gln Lys 
1               5                   10                  15      


Asn Gly Ser Trp Lys Leu Glu Lys Ala Ser Leu Ile Glu Val Lys Thr 
            20                  25                  30          


Ala Thr Trp Pro Lys Ser His Thr Leu Trp Ser Asn Gly Val Leu Glu 
        35                  40                  45              


Ser Asp Met Ile Ile Pro Lys Ser Leu Ala Gly Pro Ile Ser Gln His 
    50                  55                  60                  


Asn Tyr Arg Pro Gly Tyr His Thr Gln Thr Ala Gly Pro Trp His Leu 
65                  70                  75                  80  


Gly Lys Leu Glu Leu Asp Phe Asn Tyr Cys Glu Gly Thr Thr Val Val 
                85                  90                  95      


Ile Thr Glu Asn Cys Gly Thr Arg Gly Pro Ser Leu Arg Thr Thr Thr 
            100                 105                 110         


Val Ser Gly Lys Leu Ile His Glu Trp Cys Cys Arg Ser Cys Thr Leu 
        115                 120                 125             


Pro Pro Leu Arg Tyr Met Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu 
    130                 135                 140                 


Ile Arg Pro Ile Asn Glu Lys Glu Glu Asn Met Val Lys Ser Leu Val 
145                 150                 155                 160 


Ser Ala 
        


<210>  27
<211>  162
<212>  PRT
<213>  Dengue virus type 4

<400>  27

Gln Lys Ala Val His Ala Asp Met Gly Tyr Trp Ile Glu Ser Ser Lys 
1               5                   10                  15      


Asn Gln Thr Trp Gln Ile Glu Lys Ala Ser Leu Ile Glu Val Lys Thr 
            20                  25                  30          


Ala Leu Trp Pro Lys Thr His Thr Leu Trp Ser Asn Gly Val Leu Glu 
        35                  40                  45              


Ser Gln Met Leu Ile Pro Arg Ser Tyr Ala Gly Pro Phe Ser Gln His 
    50                  55                  60                  


Asn Tyr Arg Gln Gly Tyr Ala Thr Gln Thr Ala Gly Pro Trp His Leu 
65                  70                  75                  80  


Gly Lys Leu Glu Ile Asp Phe Gly Glu Cys Pro Gly Thr Thr Val Thr 
                85                  90                  95      


Ile Gln Glu Asp Cys Asp His Arg Gly Pro Ser Leu Arg Thr Thr Thr 
            100                 105                 110         


Ala Ser Gly Lys Leu Val Thr Gln Trp Cys Cys Arg Ser Cys Thr Met 
        115                 120                 125             


Pro Pro Leu Arg Phe Leu Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu 
    130                 135                 140                 


Ile Arg Pro Leu Ser Glu Lys Glu Glu Asn Met Val Lys Ser Gln Val 
145                 150                 155                 160 


Thr Ala 
        


<210>  28
<211>  534
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein of tandem E. coli SlyD with Dengue virus type 1 
       NS1 wing domain aa 30-180


<220>
<221>  misc_feature
<222>  (525)..(525)
<223>  Xaa can be Ala or Ser or Cys

<400>  28

Met Lys Val Ala Lys Asp Leu Val Val Ser Leu Ala Tyr Gln Val Arg 
1               5                   10                  15      


Thr Glu Asp Gly Val Leu Val Asp Glu Ser Pro Val Ser Ala Pro Leu 
            20                  25                  30          


Asp Tyr Leu His Gly His Gly Ser Leu Ile Ser Gly Leu Glu Thr Ala 
        35                  40                  45              


Leu Glu Gly His Glu Val Gly Asp Lys Phe Asp Val Ala Val Gly Ala 
    50                  55                  60                  


Asn Asp Ala Tyr Gly Gln Tyr Asp Glu Asn Leu Val Gln Arg Val Pro 
65                  70                  75                  80  


Lys Asp Val Phe Met Gly Val Asp Glu Leu Gln Val Gly Met Arg Phe 
                85                  90                  95      


Leu Ala Glu Thr Asp Gln Gly Pro Val Pro Val Glu Ile Thr Ala Val 
            100                 105                 110         


Glu Asp Asp His Val Val Val Asp Gly Asn His Met Leu Ala Gly Gln 
        115                 120                 125             


Asn Leu Lys Phe Asn Val Glu Val Val Ala Ile Arg Glu Ala Thr Glu 
    130                 135                 140                 


Glu Glu Leu Ala His Gly His Val His Gly Ala His Asp His His His 
145                 150                 155                 160 


Asp His Asp His Asp Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly 
                165                 170                 175     


Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Lys Val Ala Lys 
            180                 185                 190         


Asp Leu Val Val Ser Leu Ala Tyr Gln Val Arg Thr Glu Asp Gly Val 
        195                 200                 205             


Leu Val Asp Glu Ser Pro Val Ser Ala Pro Leu Asp Tyr Leu His Gly 
    210                 215                 220                 


His Gly Ser Leu Ile Ser Gly Leu Glu Thr Ala Leu Glu Gly His Glu 
225                 230                 235                 240 


Val Gly Asp Lys Phe Asp Val Ala Val Gly Ala Asn Asp Ala Tyr Gly 
                245                 250                 255     


Gln Tyr Asp Glu Asn Leu Val Gln Arg Val Pro Lys Asp Val Phe Met 
            260                 265                 270         


Gly Val Asp Glu Leu Gln Val Gly Met Arg Phe Leu Ala Glu Thr Asp 
        275                 280                 285             


Gln Gly Pro Val Pro Val Glu Ile Thr Ala Val Glu Asp Asp His Val 
    290                 295                 300                 


Val Val Asp Gly Asn His Met Leu Ala Gly Gln Asn Leu Lys Phe Asn 
305                 310                 315                 320 


Val Glu Val Val Ala Ile Arg Glu Ala Thr Glu Glu Glu Leu Ala His 
                325                 330                 335     


Gly His Val His Gly Ala His Asp His His His Asp His Asp His Asp 
            340                 345                 350         


Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
        355                 360                 365             


Gly Gly Gly Ser Gly Gly Gly Glu Gln Tyr Lys Phe Gln Ala Asp Ser 
    370                 375                 380                 


Pro Lys Arg Leu Ser Ala Ala Ile Gly Lys Ala Trp Glu Glu Gly Val 
385                 390                 395                 400 


Cys Gly Ile Arg Ser Ala Thr Arg Leu Glu Asn Ile Met Trp Lys Gln 
                405                 410                 415     


Ile Ser Asn Glu Leu Asn His Ile Leu Leu Glu Asn Asp Met Lys Phe 
            420                 425                 430         


Thr Val Val Val Gly Asp Val Ala Gly Ile Leu Ala Gln Gly Lys Lys 
        435                 440                 445             


Met Ile Arg Pro Gln Pro Met Glu His Lys Tyr Ser Trp Lys Ser Trp 
    450                 455                 460                 


Gly Lys Ala Lys Ile Ile Gly Ala Asp Val Gln Asn Thr Thr Phe Ile 
465                 470                 475                 480 


Ile Asp Gly Pro Asn Thr Pro Glu Cys Pro Asp Asp Gln Arg Ala Trp 
                485                 490                 495     


Asn Ile Trp Glu Val Glu Asp Tyr Gly Phe Gly Ile Phe Thr Thr Asn 
            500                 505                 510         


Ile Trp Leu Lys Leu Arg Asp Ser Tyr Thr Gln Val Xaa Asp Leu Glu 
        515                 520                 525             


His His His His His His 
    530                 


<210>  29
<211>  534
<212>  PRT
<213>  Artificial

<220>
<223>  Fusion protein of tandem E. coli SlyD with Dengue virus type 2 
       NS1 wing domain aa 30-180


<220>
<221>  misc_feature
<222>  (525)..(525)
<223>  Xaa can be Ala or Ser or Cys

<400>  29

Met Lys Val Ala Lys Asp Leu Val Val Ser Leu Ala Tyr Gln Val Arg 
1               5                   10                  15      


Thr Glu Asp Gly Val Leu Val Asp Glu Ser Pro Val Ser Ala Pro Leu 
            20                  25                  30          


Asp Tyr Leu His Gly His Gly Ser Leu Ile Ser Gly Leu Glu Thr Ala 
        35                  40                  45              


Leu Glu Gly His Glu Val Gly Asp Lys Phe Asp Val Ala Val Gly Ala 
    50                  55                  60                  


Asn Asp Ala Tyr Gly Gln Tyr Asp Glu Asn Leu Val Gln Arg Val Pro 
65                  70                  75                  80  


Lys Asp Val Phe Met Gly Val Asp Glu Leu Gln Val Gly Met Arg Phe 
                85                  90                  95      


Leu Ala Glu Thr Asp Gln Gly Pro Val Pro Val Glu Ile Thr Ala Val 
            100                 105                 110         


Glu Asp Asp His Val Val Val Asp Gly Asn His Met Leu Ala Gly Gln 
        115                 120                 125             


Asn Leu Lys Phe Asn Val Glu Val Val Ala Ile Arg Glu Ala Thr Glu 
    130                 135                 140                 


Glu Glu Leu Ala His Gly His Val His Gly Ala His Asp His His His 
145                 150                 155                 160 


Asp His Asp His Asp Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly 
                165                 170                 175     


Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Lys Val Ala Lys 
            180                 185                 190         


Asp Leu Val Val Ser Leu Ala Tyr Gln Val Arg Thr Glu Asp Gly Val 
        195                 200                 205             


Leu Val Asp Glu Ser Pro Val Ser Ala Pro Leu Asp Tyr Leu His Gly 
    210                 215                 220                 


His Gly Ser Leu Ile Ser Gly Leu Glu Thr Ala Leu Glu Gly His Glu 
225                 230                 235                 240 


Val Gly Asp Lys Phe Asp Val Ala Val Gly Ala Asn Asp Ala Tyr Gly 
                245                 250                 255     


Gln Tyr Asp Glu Asn Leu Val Gln Arg Val Pro Lys Asp Val Phe Met 
            260                 265                 270         


Gly Val Asp Glu Leu Gln Val Gly Met Arg Phe Leu Ala Glu Thr Asp 
        275                 280                 285             


Gln Gly Pro Val Pro Val Glu Ile Thr Ala Val Glu Asp Asp His Val 
    290                 295                 300                 


Val Val Asp Gly Asn His Met Leu Ala Gly Gln Asn Leu Lys Phe Asn 
305                 310                 315                 320 


Val Glu Val Val Ala Ile Arg Glu Ala Thr Glu Glu Glu Leu Ala His 
                325                 330                 335     


Gly His Val His Gly Ala His Asp His His His Asp His Asp His Asp 
            340                 345                 350         


Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
        355                 360                 365             


Gly Gly Gly Ser Gly Gly Gly Glu Gln Tyr Lys Phe Gln Pro Glu Ser 
    370                 375                 380                 


Pro Ser Lys Leu Ala Ser Ala Ile Gln Lys Ala His Glu Glu Gly Ile 
385                 390                 395                 400 


Cys Gly Ile Arg Ser Val Thr Arg Leu Glu Asn Leu Met Trp Lys Gln 
                405                 410                 415     


Ile Thr Pro Glu Leu Asn His Ile Leu Ser Glu Asn Glu Val Lys Leu 
            420                 425                 430         


Thr Ile Met Thr Gly Asp Ile Lys Gly Ile Met Gln Ala Gly Lys Arg 
        435                 440                 445             


Ser Leu Arg Pro Gln Pro Thr Glu Leu Lys Tyr Ser Trp Lys Thr Trp 
    450                 455                 460                 


Gly Lys Ala Lys Met Leu Ser Thr Glu Ser His Asn Gln Thr Phe Leu 
465                 470                 475                 480 


Ile Asp Gly Pro Glu Thr Ala Glu Cys Pro Asn Thr Asn Arg Ala Trp 
                485                 490                 495     


Asn Ser Leu Glu Val Glu Asp Tyr Gly Phe Gly Val Phe Thr Thr Asn 
            500                 505                 510         


Ile Trp Leu Lys Leu Lys Glu Lys Gln Asp Val Phe Xaa Asp Leu Glu 
        515                 520                 525             


His His His His His His 
    530                 


<210>  30
<211>  534
<212>  PRT
<213>  artificial

<220>
<223>  Fusion protein of tandem E. coli SlyD with Dengue virus type 3 
       NS1 wing domain aa 30-180


<220>
<221>  misc_feature
<222>  (525)..(525)
<223>  Xaa can be Ala or Ser or Cys

<400>  30

Met Lys Val Ala Lys Asp Leu Val Val Ser Leu Ala Tyr Gln Val Arg 
1               5                   10                  15      


Thr Glu Asp Gly Val Leu Val Asp Glu Ser Pro Val Ser Ala Pro Leu 
            20                  25                  30          


Asp Tyr Leu His Gly His Gly Ser Leu Ile Ser Gly Leu Glu Thr Ala 
        35                  40                  45              


Leu Glu Gly His Glu Val Gly Asp Lys Phe Asp Val Ala Val Gly Ala 
    50                  55                  60                  


Asn Asp Ala Tyr Gly Gln Tyr Asp Glu Asn Leu Val Gln Arg Val Pro 
65                  70                  75                  80  


Lys Asp Val Phe Met Gly Val Asp Glu Leu Gln Val Gly Met Arg Phe 
                85                  90                  95      


Leu Ala Glu Thr Asp Gln Gly Pro Val Pro Val Glu Ile Thr Ala Val 
            100                 105                 110         


Glu Asp Asp His Val Val Val Asp Gly Asn His Met Leu Ala Gly Gln 
        115                 120                 125             


Asn Leu Lys Phe Asn Val Glu Val Val Ala Ile Arg Glu Ala Thr Glu 
    130                 135                 140                 


Glu Glu Leu Ala His Gly His Val His Gly Ala His Asp His His His 
145                 150                 155                 160 


Asp His Asp His Asp Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly 
                165                 170                 175     


Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Lys Val Ala Lys 
            180                 185                 190         


Asp Leu Val Val Ser Leu Ala Tyr Gln Val Arg Thr Glu Asp Gly Val 
        195                 200                 205             


Leu Val Asp Glu Ser Pro Val Ser Ala Pro Leu Asp Tyr Leu His Gly 
    210                 215                 220                 


His Gly Ser Leu Ile Ser Gly Leu Glu Thr Ala Leu Glu Gly His Glu 
225                 230                 235                 240 


Val Gly Asp Lys Phe Asp Val Ala Val Gly Ala Asn Asp Ala Tyr Gly 
                245                 250                 255     


Gln Tyr Asp Glu Asn Leu Val Gln Arg Val Pro Lys Asp Val Phe Met 
            260                 265                 270         


Gly Val Asp Glu Leu Gln Val Gly Met Arg Phe Leu Ala Glu Thr Asp 
        275                 280                 285             


Gln Gly Pro Val Pro Val Glu Ile Thr Ala Val Glu Asp Asp His Val 
    290                 295                 300                 


Val Val Asp Gly Asn His Met Leu Ala Gly Gln Asn Leu Lys Phe Asn 
305                 310                 315                 320 


Val Glu Val Val Ala Ile Arg Glu Ala Thr Glu Glu Glu Leu Ala His 
                325                 330                 335     


Gly His Val His Gly Ala His Asp His His His Asp His Asp His Asp 
            340                 345                 350         


Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
        355                 360                 365             


Gly Gly Gly Ser Gly Gly Gly Glu Gln Tyr Lys Phe Gln Ala Asp Ser 
    370                 375                 380                 


Pro Lys Arg Leu Ala Thr Ala Ile Ala Gly Ala Trp Glu Asn Gly Val 
385                 390                 395                 400 


Cys Gly Ile Arg Ser Thr Thr Arg Met Glu Asn Leu Leu Trp Lys Gln 
                405                 410                 415     


Ile Ala Asn Glu Leu Asn Tyr Ile Leu Trp Glu Asn Asn Ile Lys Leu 
            420                 425                 430         


Thr Val Val Val Gly Asp Ile Ile Gly Ile Leu Glu Gln Gly Lys Arg 
        435                 440                 445             


Thr Leu Thr Pro Gln Pro Met Glu Leu Lys Tyr Ser Trp Lys Thr Trp 
    450                 455                 460                 


Gly Lys Ala Lys Ile Val Thr Ala Glu Thr Gln Asn Ser Ser Phe Ile 
465                 470                 475                 480 


Ile Asp Gly Pro Asn Thr Pro Glu Cys Pro Asn Ala Ser Arg Ala Trp 
                485                 490                 495     


Asn Val Trp Glu Val Glu Asp Tyr Gly Phe Gly Val Phe Thr Thr Asn 
            500                 505                 510         


Ile Trp Leu Lys Leu Arg Glu Met Tyr Ser Gln Leu Xaa Asp Leu Glu 
        515                 520                 525             


His His His His His His 
    530                 


<210>  31
<211>  534
<212>  PRT
<213>  Artificial

<220>
<223>  Fusion protein of tandem E. coli SlyD with Dengue virus type 4 
       NS1 wing domain aa 30-180


<220>
<221>  misc_feature
<222>  (525)..(525)
<223>  Xaa can be Ala or Ser or Cys

<400>  31

Met Lys Val Ala Lys Asp Leu Val Val Ser Leu Ala Tyr Gln Val Arg 
1               5                   10                  15      


Thr Glu Asp Gly Val Leu Val Asp Glu Ser Pro Val Ser Ala Pro Leu 
            20                  25                  30          


Asp Tyr Leu His Gly His Gly Ser Leu Ile Ser Gly Leu Glu Thr Ala 
        35                  40                  45              


Leu Glu Gly His Glu Val Gly Asp Lys Phe Asp Val Ala Val Gly Ala 
    50                  55                  60                  


Asn Asp Ala Tyr Gly Gln Tyr Asp Glu Asn Leu Val Gln Arg Val Pro 
65                  70                  75                  80  


Lys Asp Val Phe Met Gly Val Asp Glu Leu Gln Val Gly Met Arg Phe 
                85                  90                  95      


Leu Ala Glu Thr Asp Gln Gly Pro Val Pro Val Glu Ile Thr Ala Val 
            100                 105                 110         


Glu Asp Asp His Val Val Val Asp Gly Asn His Met Leu Ala Gly Gln 
        115                 120                 125             


Asn Leu Lys Phe Asn Val Glu Val Val Ala Ile Arg Glu Ala Thr Glu 
    130                 135                 140                 


Glu Glu Leu Ala His Gly His Val His Gly Ala His Asp His His His 
145                 150                 155                 160 


Asp His Asp His Asp Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly 
                165                 170                 175     


Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Lys Val Ala Lys 
            180                 185                 190         


Asp Leu Val Val Ser Leu Ala Tyr Gln Val Arg Thr Glu Asp Gly Val 
        195                 200                 205             


Leu Val Asp Glu Ser Pro Val Ser Ala Pro Leu Asp Tyr Leu His Gly 
    210                 215                 220                 


His Gly Ser Leu Ile Ser Gly Leu Glu Thr Ala Leu Glu Gly His Glu 
225                 230                 235                 240 


Val Gly Asp Lys Phe Asp Val Ala Val Gly Ala Asn Asp Ala Tyr Gly 
                245                 250                 255     


Gln Tyr Asp Glu Asn Leu Val Gln Arg Val Pro Lys Asp Val Phe Met 
            260                 265                 270         


Gly Val Asp Glu Leu Gln Val Gly Met Arg Phe Leu Ala Glu Thr Asp 
        275                 280                 285             


Gln Gly Pro Val Pro Val Glu Ile Thr Ala Val Glu Asp Asp His Val 
    290                 295                 300                 


Val Val Asp Gly Asn His Met Leu Ala Gly Gln Asn Leu Lys Phe Asn 
305                 310                 315                 320 


Val Glu Val Val Ala Ile Arg Glu Ala Thr Glu Glu Glu Leu Ala His 
                325                 330                 335     


Gly His Val His Gly Ala His Asp His His His Asp His Asp His Asp 
            340                 345                 350         


Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
        355                 360                 365             


Gly Gly Gly Ser Gly Gly Gly Glu Gln Tyr Lys Phe Gln Pro Glu Ser 
    370                 375                 380                 


Pro Ala Arg Leu Ala Ser Ala Ile Leu Asn Ala His Lys Asp Gly Val 
385                 390                 395                 400 


Cys Gly Ile Arg Ser Thr Thr Arg Leu Glu Asn Ile Met Trp Lys Gln 
                405                 410                 415     


Ile Thr Asn Glu Leu Asn Tyr Val Leu Trp Glu Gly Gly His Asp Leu 
            420                 425                 430         


Thr Val Val Ala Gly Asp Val Lys Gly Val Leu Thr Lys Gly Lys Arg 
        435                 440                 445             


Ala Leu Thr Pro Pro Val Asn Asp Leu Lys Tyr Ser Trp Lys Thr Trp 
    450                 455                 460                 


Gly Lys Ala Lys Ile Phe Thr Pro Glu Ala Arg Asn Ser Thr Phe Leu 
465                 470                 475                 480 


Ile Asp Gly Pro Asp Thr Ser Glu Cys Pro Asn Glu Arg Arg Ala Trp 
                485                 490                 495     


Asn Phe Phe Glu Val Glu Asp Tyr Gly Phe Gly Met Phe Thr Thr Asn 
            500                 505                 510         


Ile Trp Met Lys Phe Arg Glu Gly Ser Ser Glu Val Xaa Asp Leu Glu 
        515                 520                 525             


His His His His His His 
    530                 


<210>  32
<211>  358
<212>  PRT
<213>  Artificial

<220>
<223>  Fusion protein of tandem E. coli SlyD with Dengue virus type 1 
       NS1 -ladder domain aa 191-352

<400>  32

Met Lys Val Ala Lys Asp Leu Val Val Ser Leu Ala Tyr Gln Val Arg 
1               5                   10                  15      


Thr Glu Asp Gly Val Leu Val Asp Glu Ser Pro Val Ser Ala Pro Leu 
            20                  25                  30          


Asp Tyr Leu His Gly His Gly Ser Leu Ile Ser Gly Leu Glu Thr Ala 
        35                  40                  45              


Leu Glu Gly His Glu Val Gly Asp Lys Phe Asp Val Ala Val Gly Ala 
    50                  55                  60                  


Asn Asp Ala Tyr Gly Gln Tyr Asp Glu Asn Leu Val Gln Arg Val Pro 
65                  70                  75                  80  


Lys Asp Val Phe Met Gly Val Asp Glu Leu Gln Val Gly Met Arg Phe 
                85                  90                  95      


Leu Ala Glu Thr Asp Gln Gly Pro Val Pro Val Glu Ile Thr Ala Val 
            100                 105                 110         


Glu Asp Asp His Val Val Val Asp Gly Asn His Met Leu Ala Gly Gln 
        115                 120                 125             


Asn Leu Lys Phe Asn Val Glu Val Val Ala Ile Arg Glu Ala Thr Glu 
    130                 135                 140                 


Glu Glu Leu Ala His Gly His Val His Gly Ala His Asp His His His 
145                 150                 155                 160 


Asp His Asp His Asp Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly 
                165                 170                 175     


Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Lys Ala Val 
            180                 185                 190         


His Ala Asp Met Gly Tyr Trp Ile Glu Ser Glu Lys Asn Glu Thr Trp 
        195                 200                 205             


Lys Leu Ala Arg Ala Ser Phe Ile Glu Val Lys Thr Ala Ile Trp Pro 
    210                 215                 220                 


Lys Ser His Thr Leu Trp Ser Asn Gly Val Leu Glu Ser Glu Met Ile 
225                 230                 235                 240 


Ile Pro Lys Ile Tyr Gly Gly Pro Ile Ser Gln His Asn Tyr Arg Pro 
                245                 250                 255     


Gly Tyr Phe Thr Gln Thr Ala Gly Pro Trp His Leu Gly Lys Leu Glu 
            260                 265                 270         


Leu Asp Phe Asp Leu Cys Glu Gly Thr Thr Val Val Val Asp Glu His 
        275                 280                 285             


Cys Gly Asn Arg Gly Pro Ser Leu Arg Thr Thr Ser Val Thr Gly Lys 
    290                 295                 300                 


Ile Ile His Glu Trp Cys Cys Arg Ser Cys Thr Leu Pro Pro Leu Arg 
305                 310                 315                 320 


Phe Arg Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg Pro Val 
                325                 330                 335     


Lys Glu Lys Glu Glu Asn Leu Val Lys Ser Met Val Ser Ala Leu Glu 
            340                 345                 350         


His His His His His His 
        355             


<210>  33
<211>  358
<212>  PRT
<213>  Artificial

<220>
<223>  Fusion protein of tandem E. coli SlyD with Dengue virus type 2 
       NS1 -ladder domain aa 191-352

<400>  33

Met Lys Val Ala Lys Asp Leu Val Val Ser Leu Ala Tyr Gln Val Arg 
1               5                   10                  15      


Thr Glu Asp Gly Val Leu Val Asp Glu Ser Pro Val Ser Ala Pro Leu 
            20                  25                  30          


Asp Tyr Leu His Gly His Gly Ser Leu Ile Ser Gly Leu Glu Thr Ala 
        35                  40                  45              


Leu Glu Gly His Glu Val Gly Asp Lys Phe Asp Val Ala Val Gly Ala 
    50                  55                  60                  


Asn Asp Ala Tyr Gly Gln Tyr Asp Glu Asn Leu Val Gln Arg Val Pro 
65                  70                  75                  80  


Lys Asp Val Phe Met Gly Val Asp Glu Leu Gln Val Gly Met Arg Phe 
                85                  90                  95      


Leu Ala Glu Thr Asp Gln Gly Pro Val Pro Val Glu Ile Thr Ala Val 
            100                 105                 110         


Glu Asp Asp His Val Val Val Asp Gly Asn His Met Leu Ala Gly Gln 
        115                 120                 125             


Asn Leu Lys Phe Asn Val Glu Val Val Ala Ile Arg Glu Ala Thr Glu 
    130                 135                 140                 


Glu Glu Leu Ala His Gly His Val His Gly Ala His Asp His His His 
145                 150                 155                 160 


Asp His Asp His Asp Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly 
                165                 170                 175     


Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Asn Arg Ala Val 
            180                 185                 190         


His Ala Asp Met Gly Tyr Trp Ile Glu Ser Ala Leu Asn Asp Thr Trp 
        195                 200                 205             


Lys Ile Glu Lys Ala Ser Phe Ile Glu Val Lys Asn Ala His Trp Pro 
    210                 215                 220                 


Lys Ser His Thr Leu Trp Ser Asn Gly Val Leu Glu Ser Glu Met Ile 
225                 230                 235                 240 


Ile Pro Lys Asn Leu Ala Gly Pro Val Ser Gln His Asn Tyr Arg Pro 
                245                 250                 255     


Gly Tyr His Thr Gln Ile Ala Gly Pro Trp His Leu Gly Lys Leu Glu 
            260                 265                 270         


Met Asp Phe Asp Phe Cys Asp Gly Thr Thr Val Val Val Thr Glu Asp 
        275                 280                 285             


Cys Gly Asn Arg Gly Pro Ser Leu Arg Thr Thr Thr Ala Ser Gly Lys 
    290                 295                 300                 


Leu Ile Thr Glu Trp Cys Cys Arg Ser Cys Thr Leu Pro Pro Leu Arg 
305                 310                 315                 320 


Tyr Arg Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg Pro Leu 
                325                 330                 335     


Lys Glu Lys Glu Glu Asn Leu Val Asn Ser Leu Val Thr Ala Leu Glu 
            340                 345                 350         


His His His His His His 
        355             


<210>  34
<211>  358
<212>  PRT
<213>  Artificial

<220>
<223>  Fusion protein of tandem E. coli SlyD with Dengue virus type 3 
       NS1 -ladder domain aa 191-352

<400>  34

Met Lys Val Ala Lys Asp Leu Val Val Ser Leu Ala Tyr Gln Val Arg 
1               5                   10                  15      


Thr Glu Asp Gly Val Leu Val Asp Glu Ser Pro Val Ser Ala Pro Leu 
            20                  25                  30          


Asp Tyr Leu His Gly His Gly Ser Leu Ile Ser Gly Leu Glu Thr Ala 
        35                  40                  45              


Leu Glu Gly His Glu Val Gly Asp Lys Phe Asp Val Ala Val Gly Ala 
    50                  55                  60                  


Asn Asp Ala Tyr Gly Gln Tyr Asp Glu Asn Leu Val Gln Arg Val Pro 
65                  70                  75                  80  


Lys Asp Val Phe Met Gly Val Asp Glu Leu Gln Val Gly Met Arg Phe 
                85                  90                  95      


Leu Ala Glu Thr Asp Gln Gly Pro Val Pro Val Glu Ile Thr Ala Val 
            100                 105                 110         


Glu Asp Asp His Val Val Val Asp Gly Asn His Met Leu Ala Gly Gln 
        115                 120                 125             


Asn Leu Lys Phe Asn Val Glu Val Val Ala Ile Arg Glu Ala Thr Glu 
    130                 135                 140                 


Glu Glu Leu Ala His Gly His Val His Gly Ala His Asp His His His 
145                 150                 155                 160 


Asp His Asp His Asp Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly 
                165                 170                 175     


Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Glu Arg Ala Val 
            180                 185                 190         


His Ala Asp Met Gly Tyr Trp Ile Glu Ser Gln Lys Asn Gly Ser Trp 
        195                 200                 205             


Lys Leu Glu Lys Ala Ser Leu Ile Glu Val Lys Thr Ala Thr Trp Pro 
    210                 215                 220                 


Lys Ser His Thr Leu Trp Ser Asn Gly Val Leu Glu Ser Asp Met Ile 
225                 230                 235                 240 


Ile Pro Lys Ser Leu Ala Gly Pro Ile Ser Gln His Asn Tyr Arg Pro 
                245                 250                 255     


Gly Tyr His Thr Gln Thr Ala Gly Pro Trp His Leu Gly Lys Leu Glu 
            260                 265                 270         


Leu Asp Phe Asn Tyr Cys Glu Gly Thr Thr Val Val Ile Thr Glu Asn 
        275                 280                 285             


Cys Gly Thr Arg Gly Pro Ser Leu Arg Thr Thr Thr Val Ser Gly Lys 
    290                 295                 300                 


Leu Ile His Glu Trp Cys Cys Arg Ser Cys Thr Leu Pro Pro Leu Arg 
305                 310                 315                 320 


Tyr Met Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg Pro Ile 
                325                 330                 335     


Asn Glu Lys Glu Glu Asn Met Val Lys Ser Leu Val Ser Ala Leu Glu 
            340                 345                 350         


His His His His His His 
        355             


<210>  35
<211>  358
<212>  PRT
<213>  Artificial

<220>
<223>  Fusion protein of tandem E. coli SlyD with Dengue virus type 4 
       NS1 -ladder domain aa 191-352

<400>  35

Met Lys Val Ala Lys Asp Leu Val Val Ser Leu Ala Tyr Gln Val Arg 
1               5                   10                  15      


Thr Glu Asp Gly Val Leu Val Asp Glu Ser Pro Val Ser Ala Pro Leu 
            20                  25                  30          


Asp Tyr Leu His Gly His Gly Ser Leu Ile Ser Gly Leu Glu Thr Ala 
        35                  40                  45              


Leu Glu Gly His Glu Val Gly Asp Lys Phe Asp Val Ala Val Gly Ala 
    50                  55                  60                  


Asn Asp Ala Tyr Gly Gln Tyr Asp Glu Asn Leu Val Gln Arg Val Pro 
65                  70                  75                  80  


Lys Asp Val Phe Met Gly Val Asp Glu Leu Gln Val Gly Met Arg Phe 
                85                  90                  95      


Leu Ala Glu Thr Asp Gln Gly Pro Val Pro Val Glu Ile Thr Ala Val 
            100                 105                 110         


Glu Asp Asp His Val Val Val Asp Gly Asn His Met Leu Ala Gly Gln 
        115                 120                 125             


Asn Leu Lys Phe Asn Val Glu Val Val Ala Ile Arg Glu Ala Thr Glu 
    130                 135                 140                 


Glu Glu Leu Ala His Gly His Val His Gly Ala His Asp His His His 
145                 150                 155                 160 


Asp His Asp His Asp Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly 
                165                 170                 175     


Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Gln Lys Ala Val 
            180                 185                 190         


His Ala Asp Met Gly Tyr Trp Ile Glu Ser Ser Lys Asn Gln Thr Trp 
        195                 200                 205             


Gln Ile Glu Lys Ala Ser Leu Ile Glu Val Lys Thr Ala Leu Trp Pro 
    210                 215                 220                 


Lys Thr His Thr Leu Trp Ser Asn Gly Val Leu Glu Ser Gln Met Leu 
225                 230                 235                 240 


Ile Pro Arg Ser Tyr Ala Gly Pro Phe Ser Gln His Asn Tyr Arg Gln 
                245                 250                 255     


Gly Tyr Ala Thr Gln Thr Ala Gly Pro Trp His Leu Gly Lys Leu Glu 
            260                 265                 270         


Ile Asp Phe Gly Glu Cys Pro Gly Thr Thr Val Thr Ile Gln Glu Asp 
        275                 280                 285             


Cys Asp His Arg Gly Pro Ser Leu Arg Thr Thr Thr Ala Ser Gly Lys 
    290                 295                 300                 


Leu Val Thr Gln Trp Cys Cys Arg Ser Cys Thr Met Pro Pro Leu Arg 
305                 310                 315                 320 


Phe Leu Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg Pro Leu 
                325                 330                 335     


Ser Glu Lys Glu Glu Asn Met Val Lys Ser Gln Val Thr Ala Leu Glu 
            340                 345                 350         


His His His His His His 
        355             


