﻿序列表
<110> 深圳华大基因研究院 深圳华大基因科技有限公司
<120> Phi29 DNA聚合酶及其编码基因与应用
<160>  4   
<170>  PatentIn version 3.5

<210>  1
<211>  1719
<212>  DNA
<213>  人工序列
<220>
<223>
<400>  1
atgccgcgca aaatgtatag ctgcgacttt gaaaccacca ccaaagtgga agattgccgc       60
gtttgggcgt atggctatat gaacatcgaa gaccacagcg aatacaaaat tggcaacagc      120
ctggatgaat ttatggcgtg ggtgctgaaa gttcaggcgg atctgtattt tcacaacctg      180
aaatttgacg gcgcgttcat tattaactgg ctggaacgca acggctttaa atggagcgcg      240
gatggcttac cgaacaccta taacaccatt attagccgca tgggccagtg gtatatgatt      300
gatatctgcc tgggctataa aggcaaacgc aagattcata ccgtgatcta tgatagcctg      360
aagaaactgc cgtttccggt gaaaaaaatc gcgaaggact ttaaactgac cgtgctgaaa      420
ggcgatattg actaccataa agaacgcccg gtgggctata aaattacccc ggaggaatat      480
gcgtacatca agaacgacat ccagattatt gcggaagcgc tgctgattca gtttaaacag      540
ggcctggatc gtatgaccgc gggtagcgat agcctgaaag gctttaagga cattatcacc      600
accaagaagt tcaagaaagt gtttccgacc ctgagcctgg gcctggataa agaagtgcgc      660
tatgcgtatc gcggtggctt tacctggctg aacgatcgct ttaaggaaaa ggaaattggc      720
gaaggcatgg tgtttgatgt gaacagcctg tatccggcgc agatgtatag ccgcctgctg      780
ccgtatggtg aaccgattgt gtttgaaggc aagtatgtgt gggatgaaga ttatccgctg      840
cacattcagc atattcgctg cgaattcgaa ctgaaggaag gctatattcc gaccattcag      900
attaaacgca gccgctttta taaaggcaac gagtacctga aaagcagcgg cggcgaaatt      960
gcggatctgt ggctgagcaa cgtggatctg gaactgatga aagaacacta cgatctgtac     1020
aacgtggaat atatcagcgg cctgaaattt aaagcgacca ccggcctgtt taaggacttt     1080
atcgacaagt ggacctacat taaaaccacc agcgaaggcg cgattaaaca gctggcgaaa     1140
ctgatgctga acagcctgta tggcaaattt gcgagcaacc cggatgttac cggcaaagtg     1200
ccgtatctga aagaaaacgg cgcgctgggc tttcgtttag gcgaagagga aaccaaagat     1260
ccggtgtata ccccgatggg cgtgtttatt accgcgtggg cgcgctatac caccattacc     1320
gcggcgcagg cgtgttatga tcgcattatc tattgcgata ccgatagcat tcatctgacc     1380
ggcaccgaaa ttccggatgt gatcaaagat attgtggacc cgaaaaaact gggctattgg     1440
gcgcatgaaa gcacctttaa acgcgcgaaa tatctgcgcc agaaaaccta tatccaggac     1500
atctacatga aagaggtgga tggcaaactg gttgaaggca gcccggatga ttataccgat     1560
attaagttca gcgtgaaatg cgcgggcatg accgataaaa ttaagaagga agtgaccttc     1620
gagaacttta aagtgggctt tagccgcaaa atgaaaccga aaccggttca ggtgcctggc     1680
ggtgttgttc tggtggatga taccttcacc atcaagtga                           1719

<210>  2
<211>  572
<212>  PRT
<213>  人工序列
<220>
<223>
<400>  2
Met Pro Arg Lys Met Tyr Ser Cys Asp Phe Glu Thr Thr Thr Lys Val 
1               5                   10                  15      
Glu Asp Cys Arg Val Trp Ala Tyr Gly Tyr Met Asn Ile Glu Asp His 
            20                  25                  30          
Ser Glu Tyr Lys Ile Gly Asn Ser Leu Asp Glu Phe Met Ala Trp Val 
        35                  40                  45              
Leu Lys Val Gln Ala Asp Leu Tyr Phe His Asn Leu Lys Phe Asp Gly 
    50                  55                  60                  
Ala Phe Ile Ile Asn Trp Leu Glu Arg Asn Gly Phe Lys Trp Ser Ala 
65                  70                  75                  80  
Asp Gly Leu Pro Asn Thr Tyr Asn Thr Ile Ile Ser Arg Met Gly Gln 
                85                  90                  95      
Trp Tyr Met Ile Asp Ile Cys Leu Gly Tyr Lys Gly Lys Arg Lys Ile 
            100                 105                 110         
His Thr Val Ile Tyr Asp Ser Leu Lys Lys Leu Pro Phe Pro Val Lys 
        115                 120                 125             
Lys Ile Ala Lys Asp Phe Lys Leu Thr Val Leu Lys Gly Asp Ile Asp 
    130                 135                 140                 
Tyr His Lys Glu Arg Pro Val Gly Tyr Lys Ile Thr Pro Glu Glu Tyr 
145                 150                 155                 160 
Ala Tyr Ile Lys Asn Asp Ile Gln Ile Ile Ala Glu Ala Leu Leu Ile 
                165                 170                 175     
Gln Phe Lys Gln Gly Leu Asp Arg Met Thr Ala Gly Ser Asp Ser Leu 
            180                 185                 190         
Lys Gly Phe Lys Asp Ile Ile Thr Thr Lys Lys Phe Lys Lys Val Phe 
        195                 200                 205             
Pro Thr Leu Ser Leu Gly Leu Asp Lys Glu Val Arg Tyr Ala Tyr Arg 
    210                 215                 220                 
Gly Gly Phe Thr Trp Leu Asn Asp Arg Phe Lys Glu Lys Glu Ile Gly 
225                 230                 235                 240 
Glu Gly Met Val Phe Asp Val Asn Ser Leu Tyr Pro Ala Gln Met Tyr 
                245                 250                 255     
Ser Arg Leu Leu Pro Tyr Gly Glu Pro Ile Val Phe Glu Gly Lys Tyr 
            260                 265                 270         
Val Trp Asp Glu Asp Tyr Pro Leu His Ile Gln His Ile Arg Cys Glu 
        275                 280                 285             
Phe Glu Leu Lys Glu Gly Tyr Ile Pro Thr Ile Gln Ile Lys Arg Ser 
    290                 295                 300                 
Arg Phe Tyr Lys Gly Asn Glu Tyr Leu Lys Ser Ser Gly Gly Glu Ile 
305                 310                 315                 320 
Ala Asp Leu Trp Leu Ser Asn Val Asp Leu Glu Leu Met Lys Glu His 
                325                 330                 335     
Tyr Asp Leu Tyr Asn Val Glu Tyr Ile Ser Gly Leu Lys Phe Lys Ala 
            340                 345                 350         
Thr Thr Gly Leu Phe Lys Asp Phe Ile Asp Lys Trp Thr Tyr Ile Lys 
        355                 360                 365             
Thr Thr Ser Glu Gly Ala Ile Lys Gln Leu Ala Lys Leu Met Leu Asn 
    370                 375                 380                 
Ser Leu Tyr Gly Lys Phe Ala Ser Asn Pro Asp Val Thr Gly Lys Val 
385                 390                 395                 400 
Pro Tyr Leu Lys Glu Asn Gly Ala Leu Gly Phe Arg Leu Gly Glu Glu 
                405                 410                 415     
Glu Thr Lys Asp Pro Val Tyr Thr Pro Met Gly Val Phe Ile Thr Ala 
            420                 425                 430         
Trp Ala Arg Tyr Thr Thr Ile Thr Ala Ala Gln Ala Cys Tyr Asp Arg 
        435                 440                 445             
Ile Ile Tyr Cys Asp Thr Asp Ser Ile His Leu Thr Gly Thr Glu Ile 
    450                 455                 460                 
Pro Asp Val Ile Lys Asp Ile Val Asp Pro Lys Lys Leu Gly Tyr Trp 
465                 470                 475                 480 
Ala His Glu Ser Thr Phe Lys Arg Ala Lys Tyr Leu Arg Gln Lys Thr 
                485                 490                 495     
Tyr Ile Gln Asp Ile Tyr Met Lys Glu Val Asp Gly Lys Leu Val Glu 
            500                 505                 510         
Gly Ser Pro Asp Asp Tyr Thr Asp Ile Lys Phe Ser Val Lys Cys Ala 
        515                 520                 525             
Gly Met Thr Asp Lys Ile Lys Lys Glu Val Thr Phe Glu Asn Phe Lys 
    530                 535                 540                 
Val Gly Phe Ser Arg Lys Met Lys Pro Lys Pro Val Gln Val Pro Gly 
545                 550                 555                 560 
Gly Val Val Leu Val Asp Asp Thr Phe Thr Ile Lys 
                565                 570         

<210>  3
<211>  1779
<212>  DNA
<213>  人工序列
<220>
<223>
<400>  3
atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat       60
atgccgcgca aaatgtatag ctgcgacttt gaaaccacca ccaaagtgga agattgccgc      120
gtttgggcgt atggctatat gaacatcgaa gaccacagcg aatacaaaat tggcaacagc      180
ctggatgaat ttatggcgtg ggtgctgaaa gttcaggcgg atctgtattt tcacaacctg      240
aaatttgacg gcgcgttcat tattaactgg ctggaacgca acggctttaa atggagcgcg      300
gatggcttac cgaacaccta taacaccatt attagccgca tgggccagtg gtatatgatt      360
gatatctgcc tgggctataa aggcaaacgc aagattcata ccgtgatcta tgatagcctg      420
aagaaactgc cgtttccggt gaaaaaaatc gcgaaggact ttaaactgac cgtgctgaaa      480
ggcgatattg actaccataa agaacgcccg gtgggctata aaattacccc ggaggaatat      540
gcgtacatca agaacgacat ccagattatt gcggaagcgc tgctgattca gtttaaacag      600
ggcctggatc gtatgaccgc gggtagcgat agcctgaaag gctttaagga cattatcacc      660
accaagaagt tcaagaaagt gtttccgacc ctgagcctgg gcctggataa agaagtgcgc      720
tatgcgtatc gcggtggctt tacctggctg aacgatcgct ttaaggaaaa ggaaattggc      780
gaaggcatgg tgtttgatgt gaacagcctg tatccggcgc agatgtatag ccgcctgctg      840
ccgtatggtg aaccgattgt gtttgaaggc aagtatgtgt gggatgaaga ttatccgctg      900
cacattcagc atattcgctg cgaattcgaa ctgaaggaag gctatattcc gaccattcag      960
attaaacgca gccgctttta taaaggcaac gagtacctga aaagcagcgg cggcgaaatt     1020
gcggatctgt ggctgagcaa cgtggatctg gaactgatga aagaacacta cgatctgtac     1080
aacgtggaat atatcagcgg cctgaaattt aaagcgacca ccggcctgtt taaggacttt     1140
atcgacaagt ggacctacat taaaaccacc agcgaaggcg cgattaaaca gctggcgaaa     1200
ctgatgctga acagcctgta tggcaaattt gcgagcaacc cggatgttac cggcaaagtg     1260
ccgtatctga aagaaaacgg cgcgctgggc tttcgtttag gcgaagagga aaccaaagat     1320
ccggtgtata ccccgatggg cgtgtttatt accgcgtggg cgcgctatac caccattacc     1380
gcggcgcagg cgtgttatga tcgcattatc tattgcgata ccgatagcat tcatctgacc     1440
ggcaccgaaa ttccggatgt gatcaaagat attgtggacc cgaaaaaact gggctattgg     1500
gcgcatgaaa gcacctttaa acgcgcgaaa tatctgcgcc agaaaaccta tatccaggac     1560
atctacatga aagaggtgga tggcaaactg gttgaaggca gcccggatga ttataccgat     1620
attaagttca gcgtgaaatg cgcgggcatg accgataaaa ttaagaagga agtgaccttc     1680
gagaacttta aagtgggctt tagccgcaaa atgaaaccga aaccggttca ggtgcctggc     1740
ggtgttgttc tggtggatga taccttcacc atcaagtga                           1779

<210>  4
<211>  592
<212>  PRT
<213>  人工序列
<220>
<223>
<400>  4
 Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      
Arg Gly Ser His Met Pro Arg Lys Met Tyr Ser Cys Asp Phe Glu Thr 
            20                  25                  30          
Thr Thr Lys Val Glu Asp Cys Arg Val Trp Ala Tyr Gly Tyr Met Asn 
        35                  40                  45              
Ile Glu Asp His Ser Glu Tyr Lys Ile Gly Asn Ser Leu Asp Glu Phe 
    50                  55                  60                  
Met Ala Trp Val Leu Lys Val Gln Ala Asp Leu Tyr Phe His Asn Leu 
65                  70                  75                  80  
Lys Phe Asp Gly Ala Phe Ile Ile Asn Trp Leu Glu Arg Asn Gly Phe 
                85                  90                  95      
Lys Trp Ser Ala Asp Gly Leu Pro Asn Thr Tyr Asn Thr Ile Ile Ser 
            100                 105                 110         
Arg Met Gly Gln Trp Tyr Met Ile Asp Ile Cys Leu Gly Tyr Lys Gly 
        115                 120                 125             
Lys Arg Lys Ile His Thr Val Ile Tyr Asp Ser Leu Lys Lys Leu Pro 
    130                 135                 140                 
Phe Pro Val Lys Lys Ile Ala Lys Asp Phe Lys Leu Thr Val Leu Lys 
145                 150                 155                 160 
Gly Asp Ile Asp Tyr His Lys Glu Arg Pro Val Gly Tyr Lys Ile Thr 
                165                 170                 175     
Pro Glu Glu Tyr Ala Tyr Ile Lys Asn Asp Ile Gln Ile Ile Ala Glu 
            180                 185                 190         
Ala Leu Leu Ile Gln Phe Lys Gln Gly Leu Asp Arg Met Thr Ala Gly 
        195                 200                 205             
Ser Asp Ser Leu Lys Gly Phe Lys Asp Ile Ile Thr Thr Lys Lys Phe 
    210                 215                 220                 
Lys Lys Val Phe Pro Thr Leu Ser Leu Gly Leu Asp Lys Glu Val Arg 
225                 230                 235                 240 
Tyr Ala Tyr Arg Gly Gly Phe Thr Trp Leu Asn Asp Arg Phe Lys Glu 
                245                 250                 255     
Lys Glu Ile Gly Glu Gly Met Val Phe Asp Val Asn Ser Leu Tyr Pro 
            260                 265                 270         
Ala Gln Met Tyr Ser Arg Leu Leu Pro Tyr Gly Glu Pro Ile Val Phe 
        275                 280                 285             
Glu Gly Lys Tyr Val Trp Asp Glu Asp Tyr Pro Leu His Ile Gln His 
    290                 295                 300                 
Ile Arg Cys Glu Phe Glu Leu Lys Glu Gly Tyr Ile Pro Thr Ile Gln 
305                 310                 315                 320 
Ile Lys Arg Ser Arg Phe Tyr Lys Gly Asn Glu Tyr Leu Lys Ser Ser 
                325                 330                 335     
Gly Gly Glu Ile Ala Asp Leu Trp Leu Ser Asn Val Asp Leu Glu Leu 
            340                 345                 350         
Met Lys Glu His Tyr Asp Leu Tyr Asn Val Glu Tyr Ile Ser Gly Leu 
        355                 360                 365             
Lys Phe Lys Ala Thr Thr Gly Leu Phe Lys Asp Phe Ile Asp Lys Trp 
    370                 375                 380                 
Thr Tyr Ile Lys Thr Thr Ser Glu Gly Ala Ile Lys Gln Leu Ala Lys 
385                 390                 395                 400 
Leu Met Leu Asn Ser Leu Tyr Gly Lys Phe Ala Ser Asn Pro Asp Val 
                405                 410                 415     
Thr Gly Lys Val Pro Tyr Leu Lys Glu Asn Gly Ala Leu Gly Phe Arg 
            420                 425                 430         
Leu Gly Glu Glu Glu Thr Lys Asp Pro Val Tyr Thr Pro Met Gly Val 
        435                 440                 445             
Phe Ile Thr Ala Trp Ala Arg Tyr Thr Thr Ile Thr Ala Ala Gln Ala 
    450                 455                 460                 
Cys Tyr Asp Arg Ile Ile Tyr Cys Asp Thr Asp Ser Ile His Leu Thr 
465                 470                 475                 480 
Gly Thr Glu Ile Pro Asp Val Ile Lys Asp Ile Val Asp Pro Lys Lys 
                485                 490                 495     
Leu Gly Tyr Trp Ala His Glu Ser Thr Phe Lys Arg Ala Lys Tyr Leu 
            500                 505                 510         
Arg Gln Lys Thr Tyr Ile Gln Asp Ile Tyr Met Lys Glu Val Asp Gly 
        515                 520                 525             
Lys Leu Val Glu Gly Ser Pro Asp Asp Tyr Thr Asp Ile Lys Phe Ser 
    530                 535                 540                 
Val Lys Cys Ala Gly Met Thr Asp Lys Ile Lys Lys Glu Val Thr Phe 
545                 550                 555                 560 
Glu Asn Phe Lys Val Gly Phe Ser Arg Lys Met Lys Pro Lys Pro Val 
                565                 570                 575     
Gln Val Pro Gly Gly Val Val Leu Val Asp Asp Thr Phe Thr Ile Lys 
            580                 585                 590  