                         SEQUENCE LISTING

<110>  University of Massachusetts
 
<120>  Targeting Cell Tropism Receptors to Inhibit Cytomegalovirus 
       Infection

<130>  07917-0398WO1

<140>  PCT/US2018/027469
<141>  2018-04-13

<150>  US 62/485,742
<151>  2017-04-14

<160>  43    

<170>  PatentIn version 3.5

<210>  1
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal fragment of OR14I1

<400>  1

Met Asp Asn Leu Thr Lys Val Thr Glu Phe Leu Leu Met Glu Phe Ser 
1               5                   10                  15      


Gly Ile Trp Glu Leu Gln Val Leu His Ala 
            20                  25      


<210>  2
<211>  311
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Asp Asn Leu Thr Lys Val Thr Glu Phe Leu Leu Met Glu Phe Ser 
1               5                   10                  15      


Gly Ile Trp Glu Leu Gln Val Leu His Ala Gly Leu Phe Leu Leu Ile 
            20                  25                  30          


Tyr Leu Ala Val Leu Val Gly Asn Leu Leu Ile Ile Ala Val Ile Thr 
        35                  40                  45              


Leu Asp Gln His Leu His Thr Pro Met Tyr Phe Phe Leu Lys Asn Leu 
    50                  55                  60                  


Ser Val Leu Asp Leu Cys Tyr Ile Ser Val Thr Val Pro Lys Ser Ile 
65                  70                  75                  80  


Arg Asn Ser Leu Thr Arg Arg Ser Ser Ile Ser Tyr Leu Gly Cys Val 
                85                  90                  95      


Ala Gln Val Tyr Phe Phe Ser Ala Phe Ala Ser Ala Glu Leu Ala Phe 
            100                 105                 110         


Leu Thr Val Met Ser Tyr Asp Arg Tyr Val Ala Ile Cys His Pro Leu 
        115                 120                 125             


Gln Tyr Arg Ala Val Met Thr Ser Gly Gly Cys Tyr Gln Met Ala Val 
    130                 135                 140                 


Thr Thr Trp Leu Ser Cys Phe Ser Tyr Ala Ala Val His Thr Gly Asn 
145                 150                 155                 160 


Met Phe Arg Glu His Val Cys Arg Ser Ser Val Ile His Gln Phe Phe 
                165                 170                 175     


Arg Asp Ile Pro His Val Leu Ala Leu Val Ser Cys Glu Val Phe Phe 
            180                 185                 190         


Val Glu Phe Leu Thr Leu Ala Leu Ser Ser Cys Leu Val Leu Gly Cys 
        195                 200                 205             


Phe Ile Leu Met Met Ile Ser Tyr Phe Gln Ile Phe Ser Thr Val Leu 
    210                 215                 220                 


Arg Ile Pro Ser Gly Gln Ser Arg Ala Lys Ala Phe Ser Thr Cys Ser 
225                 230                 235                 240 


Pro Gln Leu Ile Val Ile Met Leu Phe Leu Thr Thr Gly Leu Phe Ala 
                245                 250                 255     


Ala Leu Gly Pro Ile Ala Lys Ala Leu Ser Ile Gln Asp Leu Val Ile 
            260                 265                 270         


Ala Leu Thr Tyr Thr Val Leu Pro Pro Phe Leu Asn Pro Ile Ile Tyr 
        275                 280                 285             


Ser Leu Arg Asn Lys Glu Ile Lys Thr Ala Met Trp Arg Leu Phe Val 
    290                 295                 300                 


Lys Ile Tyr Phe Leu Gln Lys 
305                 310     


<210>  3
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OR14I1 peptide

<400>  3

Phe Leu Leu Met Glu Phe Ser Gly Ile Trp Glu Leu Gln 
1               5                   10              


<210>  4
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OR14I1 peptide

<400>  4

Leu Leu Met Glu Phe Ser Gly Ile Trp Glu Leu Gln Val 
1               5                   10              


<210>  5
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OR14I1 peptide

<400>  5

Leu Met Glu Phe Ser Gly Ile Trp Glu Leu Gln Val Leu 
1               5                   10              


<210>  6
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OR14I1 peptide

<400>  6

Leu Met Glu Phe Ser Gly Ile Trp Glu Leu Gln 
1               5                   10      


<210>  7
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  QLEWIGSFEML

<400>  7

Gln Leu Glu Trp Ile Gly Ser Phe Glu Met Leu 
1               5                   10      


<210>  8
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer with original sequence

<400>  8
tcctacgcag ccgtccacac t                                                 21


<210>  9
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer with silent mutation sequence

<400>  9
agctatgctg ctgtgcatac c                                                 21


<210>  10
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer lentiGP-1_F

<400>  10
aatggactat catatgctta ccgtaacttg aaagtatttc g                           41


<210>  11
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer lentiGP-3_R

<400>  11
atgaatactg ccatttgtct caagatctag ttacgc                                 36


<210>  12
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  flanking sequence at 5' end

<400>  12
gaaaggacga aacacc                                                       16


<210>  13
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  flanking sequence at 3' end

<400>  13
tttctagctc taaaac                                                       16


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  guideRNA PDGFRA_1

<400>  14
gaccttcaat ggacttaccc                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  guideRNA PDGFRA_2

<400>  15
agctatgggg acttcccatc                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  guideRNA OR14I1_1

<400>  16
tcctacgcag ccgtccacac                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  guideRNA OR14I1_2

<400>  17
tgagcaccgt tgagaagatt                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  guideRNA DMBT1_1

<400>  18
ttaccgtagt ctgtagtcct                                                   20


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  guideRNA DMBT1_2

<400>  19
cgcagcttca ctgattccct                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  guideRNA CON_1

<400>  20
cgggatgcag ctggagagga                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  guideRNA CON_2

<400>  21
ccagttgctc tgggggaaca                                                   20


<210>  22
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  shOR14I1

<400>  22
gcagaagctc catctcttat c                                                 21


<210>  23
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  shDMBT1

<400>  23
ggagtcaact gtagcagaag g                                                 21


<210>  24
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  shPDGFRA

<400>  24
gcctttgtac ctctaggaat g                                                 21


<210>  25
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hCON

<400>  25
aatttttttc cccaaagggg g                                                 21


<210>  26
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OR14I1 Peptide 2

<400>  26

Pro Lys Ser Ile Arg Asn Ser Leu Thr Arg Arg Ser Ser Ile Ser Tyr 
1               5                   10                  15      


<210>  27
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OR14I1 Peptide 3

<400>  27

Arg Glu His Val Cys Arg Ser Ser Val Ile His Gln Phe Phe Arg Asp 
1               5                   10                  15      


Ile Pro His Val Leu Ala Leu Val Ser Cys Glu 
            20                  25          


<210>  28
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OR14I1 Peptide 4

<400>  28

Lys Ala Leu Ser Ile Gln Asp 
1               5           


<210>  29
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OR14I1 peptide 1-13aa

<400>  29

Met Asp Asn Leu Thr Lys Val Thr Glu Phe Leu Leu Met 
1               5                   10              


<210>  30
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OR14I1 peptide 2-14aa

<400>  30

Asp Asn Leu Thr Lys Val Thr Glu Phe Leu Leu Met Glu 
1               5                   10              


<210>  31
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OR14I1 peptide 3-15aa

<400>  31

Asn Leu Thr Lys Val Thr Glu Phe Leu Leu Met Glu Phe 
1               5                   10              


<210>  32
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OR14I1 peptide 4-16aa

<400>  32

Leu Thr Lys Val Thr Glu Phe Leu Leu Met Glu Phe Ser 
1               5                   10              


<210>  33
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OR14I1 peptide 5-17aa

<400>  33

Thr Lys Val Thr Glu Phe Leu Leu Met Glu Phe Ser Gly 
1               5                   10              


<210>  34
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OR14I1 peptide 6-18aa

<400>  34

Lys Val Thr Glu Phe Leu Leu Met Glu Phe Ser Gly Ile 
1               5                   10              


<210>  35
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OR14I1 peptide 7-19aa

<400>  35

Val Thr Glu Phe Leu Leu Met Glu Phe Ser Gly Ile Trp 
1               5                   10              


<210>  36
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OR14I1 peptide 8-20aa

<400>  36

Thr Glu Phe Leu Leu Met Glu Phe Ser Gly Ile Trp Glu 
1               5                   10              


<210>  37
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OR14I1 peptide 9-21aa

<400>  37

Glu Phe Leu Leu Met Glu Phe Ser Gly Ile Trp Glu Leu 
1               5                   10              


<210>  38
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OR14I1 peptide 10-22aa

<400>  38

Phe Leu Leu Met Glu Phe Ser Gly Ile Trp Glu Leu Gln 
1               5                   10              


<210>  39
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OR14I1 peptide 11-23aa

<400>  39

Leu Leu Met Glu Phe Ser Gly Ile Trp Glu Leu Gln Val 
1               5                   10              


<210>  40
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OR14I1 peptide 12-24aa

<400>  40

Leu Met Glu Phe Ser Gly Ile Trp Glu Leu Gln Val Leu 
1               5                   10              


<210>  41
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OR14I1 peptide 13-25aa

<400>  41

Met Glu Phe Ser Gly Ile Trp Glu Leu Gln Val Leu His 
1               5                   10              


<210>  42
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OR14I1 peptide 14-26aa

<400>  42

Glu Phe Ser Gly Ile Trp Glu Leu Gln Val Leu His Ala 
1               5                   10              


<210>  43
<211>  936
<212>  DNA
<213>  Homo sapiens

<400>  43
atggacaatc tcacaaaagt gacagaattc ctgctgatgg agttttctgg tatctgggag       60

ctgcaggtgc tgcacgccgg gctgtttctg ctgatttatc tggcagtgct ggtggggaac      120

ctgctcatca ttgcagtcat cactctcgat cagcatcttc acacacccat gtacttcttc      180

ctgaagaacc tctccgtttt ggatctgtgc tacatctcag tcactgtgcc taaatccatc      240

cgtaactccc tgactcgcag aagctccatc tcttatcttg gctgtgtggc tcaagtctat      300

tttttctctg cctttgcatc tgctgagctg gccttcctta ctgtcatgtc ttatgaccgc      360

tatgttgcca tttgccaccc cctccaatac agagccgtga tgacatcagg agggtgctat      420

cagatggcag tcaccacctg gctaagctgc ttttcctacg cagccgtcca cactggcaac      480

atgtttcggg agcacgtttg cagatccagt gtgatccacc agttcttccg tgacatccct      540

catgtgttgg ccctggtttc ctgtgaggtt ttctttgtag agtttttgac cctggccctg      600

agctcatgct tggttctggg atgctttatt ctcatgatga tctcctattt ccaaatcttc      660

tcaacggtgc tcagaatccc ttcaggacag agtcgagcaa aagccttctc cacctgctcc      720

ccccagctca ttgtcatcat gctctttctt accacagggc tctttgctgc cttaggacca      780

attgcaaaag ctctgtccat tcaggattta gtgattgctc tgacatacac agttttgcct      840

cccttcctca atcccatcat atatagtctt aggaataagg agattaaaac agccatgtgg      900

agactctttg tgaagatata ttttctgcaa aagtag                                936


