
                                SEQUENCE LISTING

<110> WYL Sciences Inc.
      Yang, George P.
      Wang, Kevin C.
      Wan, Derrick C.

<120> ANTIBODIES TO ROYALACTIN AND USES
  THEREOF

<130> WYLS.001WO

<150> 62/485263           
<151> 2017-04-13  

<160> 8

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 1299
<212> DNA
<213> Apis melifera

<220> 
<221> misc_feature   
<222> 1089
<223> n = A,T,C or G

<400> 1
atgacaagat tgtttatgct ggtatgcctt ggcatagttt gtcaaggtac gacaggcaac 60
attcttcgag gagagtcttt aaacaaatca ttacccatcc ttcacgaatg gaaattcttt 120
gattatgatt tcggtagcga tgaaagaaga caagatgcaa ttctatctgg cgaatacgac 180
tacaagaata attatccatc cgacattgac caatggcatg ataagatttt tgtcaccatg 240
ctgagataca atggcgtacc ttcctctttg aacgtgatat ctaaaaaggt cggtgatggt 300
ggtcctcttc tacaacctta tcccgattgg tcgtttgcta aatatgacga ttgctctgga 360
atcgtgagcg cctcaaaact tgcgatcgac aaatgcgaca gattgtgggt tctggactca 420
ggtcttgtca ataatactca acccatgtgt tctccaaaac tgctcacctt tgatctgact 480
acctcgcaat tgctcaagca agttgaaata ccacatgatg ttgccgtaaa tgccactaca 540
ggaaagggaa gattatcatc tctagctgtt caatctttag attgcaatac aaatagcgat 600
actatggtgt acatagcaga cgagaaaggt gaaggtttaa tcgtgtatca taattctgat 660
gattccttcc atcgattgac ttccaacact ttcgattacg atcctaaatt taccaaaatg 720
acgatcgatg gagaaagtta cacagcccaa gatggaattt ctggaatggc tcttagtccc 780
atgactaaca atctctatta cagtcctgta gcttccacca gtttgtatta tgttaacacg 840
gaacaattca gaacatccga ttatcaacag aatgacatac attacgaagg agtccaaaat 900
attttggata cccaatcgtc cgctaaagta gtatcaaaga gtggcgttct cttcttcgga 960
ttggtgggcg attcagctct tggctgctgg aacgaacatc gaacacttga aagacacaat 1020
atccgtaccg tcgctcaaag tgatgagact cttcaaatga tcgctagcat gaagattaag 1080
gaagctctnc cacacgtgcc tatattcgat aggtatataa accgtgaata catattggtt 1140
ttaagtaaca aaatgcaaaa aatggtgaat aatgacttca acttcgacga tgttaacttc 1200
agaattatga acgcgaatgt aaacgaattg atattgaaca ctcgttgcga aaatcccgat 1260
aatgatcgaa cacctttcaa aatttcaatc catttgtaa                        1299

<210> 2
<211> 432
<212> PRT
<213> Apis melifera

<220> 
<221> VARIANT        
<222> 363
<223> Xaa = Any Amino Acid

<400> 2
Met Thr Arg Leu Phe Met Leu Val Cys Leu Gly Ile Val Cys Gln Gly
 1               5                  10                  15      
Thr Thr Gly Asn Ile Leu Arg Gly Glu Ser Leu Asn Lys Ser Leu Pro
            20                  25                  30          
Ile Leu His Glu Trp Lys Phe Phe Asp Tyr Asp Phe Gly Ser Asp Glu
        35                  40                  45              
Arg Arg Gln Asp Ala Ile Leu Ser Gly Glu Tyr Asp Tyr Lys Asn Asn
    50                  55                  60                  
Tyr Pro Ser Asp Ile Asp Gln Trp His Asp Lys Ile Phe Val Thr Met
65                  70                  75                  80  
Leu Arg Tyr Asn Gly Val Pro Ser Ser Leu Asn Val Ile Ser Lys Lys
                85                  90                  95      
Val Gly Asp Gly Gly Pro Leu Leu Gln Pro Tyr Pro Asp Trp Ser Phe
            100                 105                 110         
Ala Lys Tyr Asp Asp Cys Ser Gly Ile Val Ser Ala Ser Lys Leu Ala
        115                 120                 125             
Ile Asp Lys Cys Asp Arg Leu Trp Val Leu Asp Ser Gly Leu Val Asn
    130                 135                 140                 
Asn Thr Gln Pro Met Cys Ser Pro Lys Leu Leu Thr Phe Asp Leu Thr
145                 150                 155                 160 
Thr Ser Gln Leu Leu Lys Gln Val Glu Ile Pro His Asp Val Ala Val
                165                 170                 175     
Asn Ala Thr Thr Gly Lys Gly Arg Leu Ser Ser Leu Ala Val Gln Ser
            180                 185                 190         
Leu Asp Cys Asn Thr Asn Ser Asp Thr Met Val Tyr Ile Ala Asp Glu
        195                 200                 205             
Lys Gly Glu Gly Leu Ile Val Tyr His Asn Ser Asp Asp Ser Phe His
    210                 215                 220                 
Arg Leu Thr Ser Asn Thr Phe Asp Tyr Asp Pro Lys Phe Thr Lys Met
225                 230                 235                 240 
Thr Ile Asp Gly Glu Ser Tyr Thr Ala Gln Asp Gly Ile Ser Gly Met
                245                 250                 255     
Ala Leu Ser Pro Met Thr Asn Asn Leu Tyr Tyr Ser Pro Val Ala Ser
            260                 265                 270         
Thr Ser Leu Tyr Tyr Val Asn Thr Glu Gln Phe Arg Thr Ser Asp Tyr
        275                 280                 285             
Gln Gln Asn Asp Ile His Tyr Glu Gly Val Gln Asn Ile Leu Asp Thr
    290                 295                 300                 
Gln Ser Ser Ala Lys Val Val Ser Lys Ser Gly Val Leu Phe Phe Gly
305                 310                 315                 320 
Leu Val Gly Asp Ser Ala Leu Gly Cys Trp Asn Glu His Arg Thr Leu
                325                 330                 335     
Glu Arg His Asn Ile Arg Thr Val Ala Gln Ser Asp Glu Thr Leu Gln
            340                 345                 350         
Met Ile Ala Ser Met Lys Ile Lys Glu Ala Xaa Pro His Val Pro Ile
        355                 360                 365             
Phe Asp Arg Tyr Ile Asn Arg Glu Tyr Ile Leu Val Leu Ser Asn Lys
    370                 375                 380                 
Met Gln Lys Met Val Asn Asn Asp Phe Asn Phe Asp Asp Val Asn Phe
385                 390                 395                 400 
Arg Ile Met Asn Ala Asn Val Asn Glu Leu Ile Leu Asn Thr Arg Cys
                405                 410                 415     
Glu Asn Pro Asp Asn Asp Arg Thr Pro Phe Lys Ile Ser Ile His Leu
            420                 425                 430         


<210> 3
<211> 1353
<212> DNA
<213> Artificial Sequence

<220> 
<223> Coding sequence for Flag-Royalactin-His fusion
      protein

<400> 3
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccgat 60
tacaaggacg acgatgacaa gaacattctg cgtggggaat ccctgaacaa gtcattgcct 120
atccttcatg aatggaaatt ctttgattat gattttggtt ccgacgaacg acggcaggac 180
gccatcctct ctggagaata tgattacaaa aacaactacc cttctgatat cgaccagtgg 240
cacgataaga tttttgtcac catgctgcgg tacaatggag ttccctcttc actgaacgtc 300
atcagtaaga aggtgggcga cggaggaccc ctgctccagc catatcctga ttggtctttc 360
gctaagtacg acgactgttc tggaatcgtc tccgcttcta agctggccat tgacaagtgt 420
gatcggttgt gggtcctgga ttcagggttg gtgaacaata cccagcccat gtgctctcct 480
aagctgctga ccttcgacct caccaccagc cagttgctca agcaggtgga gattccccac 540
gacgtcgctg tgaacgctac cacaggcaag ggccgcttga gcagccttgc tgtgcaaagc 600
ctggactgca acaccaattc agatactatg gtgtacatcg cagacgaaaa gggtgaaggt 660
ctgattgtct accataactc agacgatagt tttcatagac tgacctccaa cacattcgat 720
tatgacccca agttcactaa gatgactatt gacggtgagt catacactgc ccaggacggg 780
atttccggta tggcactgtc acctatgaca aataacctgt attattctcc cgttgcaagc 840
acttctctgt actacgtgaa cactgagcaa ttcaggacca gcgactatca acagaatgat 900
atccattacg agggagtcca gaacatcctt gacactcagt cctctgccaa ggtagttagc 960
aagagtggag tattgttttt cggcctggtt ggcgacagtg ctttgggatg ttggaatgaa 1020
catcggaccc tggaacgtca taacattcgc actgtggccc aatctgacga gactcttcag 1080
atgatcgcct ctatgaagat aaaggaggcc ttgccccacg tccctatctt cgacaggtat 1140
atcaaccgtg aatatatact ggtgctctca aataagatgc agaaaatggt taataatgat 1200
ttcaattttg acgatgtgaa ttttaggatc atgaacgcaa acgttaatga actgatcttg 1260
aatacccgtt gtgagaatcc cgacaacgat aggacaccct ttaagatttc tattcacctg 1320
caccaccatc atcaccatca ccaccatcac tag                              1353

<210> 4
<211> 450
<212> PRT
<213> Artificial Sequence

<220> 
<223> Flag-Royalactin-His fusion protein

<400> 4
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly
 1               5                  10                  15      
Val His Ser Asp Tyr Lys Asp Asp Asp Asp Lys Asn Ile Leu Arg Gly
            20                  25                  30          
Glu Ser Leu Asn Lys Ser Leu Pro Ile Leu His Glu Trp Lys Phe Phe
        35                  40                  45              
Asp Tyr Asp Phe Gly Ser Asp Glu Arg Arg Gln Asp Ala Ile Leu Ser
    50                  55                  60                  
Gly Glu Tyr Asp Tyr Lys Asn Asn Tyr Pro Ser Asp Ile Asp Gln Trp
65                  70                  75                  80  
His Asp Lys Ile Phe Val Thr Met Leu Arg Tyr Asn Gly Val Pro Ser
                85                  90                  95      
Ser Leu Asn Val Ile Ser Lys Lys Val Gly Asp Gly Gly Pro Leu Leu
            100                 105                 110         
Gln Pro Tyr Pro Asp Trp Ser Phe Ala Lys Tyr Asp Asp Cys Ser Gly
        115                 120                 125             
Ile Val Ser Ala Ser Lys Leu Ala Ile Asp Lys Cys Asp Arg Leu Trp
    130                 135                 140                 
Val Leu Asp Ser Gly Leu Val Asn Asn Thr Gln Pro Met Cys Ser Pro
145                 150                 155                 160 
Lys Leu Leu Thr Phe Asp Leu Thr Thr Ser Gln Leu Leu Lys Gln Val
                165                 170                 175     
Glu Ile Pro His Asp Val Ala Val Asn Ala Thr Thr Gly Lys Gly Arg
            180                 185                 190         
Leu Ser Ser Leu Ala Val Gln Ser Leu Asp Cys Asn Thr Asn Ser Asp
        195                 200                 205             
Thr Met Val Tyr Ile Ala Asp Glu Lys Gly Glu Gly Leu Ile Val Tyr
    210                 215                 220                 
His Asn Ser Asp Asp Ser Phe His Arg Leu Thr Ser Asn Thr Phe Asp
225                 230                 235                 240 
Tyr Asp Pro Lys Phe Thr Lys Met Thr Ile Asp Gly Glu Ser Tyr Thr
                245                 250                 255     
Ala Gln Asp Gly Ile Ser Gly Met Ala Leu Ser Pro Met Thr Asn Asn
            260                 265                 270         
Leu Tyr Tyr Ser Pro Val Ala Ser Thr Ser Leu Tyr Tyr Val Asn Thr
        275                 280                 285             
Glu Gln Phe Arg Thr Ser Asp Tyr Gln Gln Asn Asp Ile His Tyr Glu
    290                 295                 300                 
Gly Val Gln Asn Ile Leu Asp Thr Gln Ser Ser Ala Lys Val Val Ser
305                 310                 315                 320 
Lys Ser Gly Val Leu Phe Phe Gly Leu Val Gly Asp Ser Ala Leu Gly
                325                 330                 335     
Cys Trp Asn Glu His Arg Thr Leu Glu Arg His Asn Ile Arg Thr Val
            340                 345                 350         
Ala Gln Ser Asp Glu Thr Leu Gln Met Ile Ala Ser Met Lys Ile Lys
        355                 360                 365             
Glu Ala Leu Pro His Val Pro Ile Phe Asp Arg Tyr Ile Asn Arg Glu
    370                 375                 380                 
Tyr Ile Leu Val Leu Ser Asn Lys Met Gln Lys Met Val Asn Asn Asp
385                 390                 395                 400 
Phe Asn Phe Asp Asp Val Asn Phe Arg Ile Met Asn Ala Asn Val Asn
                405                 410                 415     
Glu Leu Ile Leu Asn Thr Arg Cys Glu Asn Pro Asp Asn Asp Arg Thr
            420                 425                 430         
Pro Phe Lys Ile Ser Ile His Leu His His His His His His His His
        435                 440                 445             
His His
    450 


<210> 5
<211> 339
<212> DNA
<213> Mus musculus

<400> 5
gtgatgctgg tggaatctgg gggaggctta gtgaagcctg gagggtccct gaaactctcc 60
tgtgcagcct ctggattcac tttcagtagg tatgccatgt cttggaatcg ccagactccg 120
gagaagaggc tggagtgggt cgcaacaatt agtcctggtg gtggttacat atactattca 180
gacagtgtga aggggcgatt caccatctcc agagacaatg ccaggaacac cctgtatctg 240
caaatgagca gtctgaggtc tgaggacacg gccatgtatt actgtgcagg ggactatgtt 300
gactattggg gccaaggcac cactctcaca gtctcctca                        339

<210> 6
<211> 113
<212> PRT
<213> Mus musculus

<400> 6
Val Met Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly Ser
 1               5                  10                  15      
Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Arg Tyr Ala
            20                  25                  30          
Met Ser Trp Asn Arg Gln Thr Pro Glu Lys Arg Leu Glu Trp Val Ala
        35                  40                  45              
Thr Ile Ser Pro Gly Gly Gly Tyr Ile Tyr Tyr Ser Asp Ser Val Lys
    50                  55                  60                  
Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Arg Asn Thr Leu Tyr Leu
65                  70                  75                  80  
Gln Met Ser Ser Leu Arg Ser Glu Asp Thr Ala Met Tyr Tyr Cys Ala
                85                  90                  95      
Gly Asp Tyr Val Asp Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val Ser
            100                 105                 110         
Ser
    


<210> 7
<211> 339
<212> DNA
<213> Mus musculus

<400> 7
gatgttgtga tgacccagac tccactcact ttgtcggtta ccactggaca accagcctcc 60
atctcttgca agtcaagtca gagcctctta aatagtgatg gaaagacatt tttgaattgg 120
ttgttacaga ggccagggca gtctccaaag cgcctaatct atctggtgtc taaactggac 180
tctggagtcc ctgacaggtt cactggcagt ggatcaggga cagatttcac actgaaaatc 240
agcagagtgg aggctgagga tttgggagtt tattattgct ggcaaggtac acattttcca 300
tacacgttcg gaggggggac caagctggaa ataaaacgg                        339

<210> 8
<211> 113
<212> PRT
<213> Mus musculus

<400> 8
Asp Val Val Met Thr Gln Thr Pro Leu Thr Leu Ser Val Thr Thr Gly
 1               5                  10                  15      
Gln Pro Ala Ser Ile Ser Cys Lys Ser Ser Gln Ser Leu Leu Asn Ser
            20                  25                  30          
Asp Gly Lys Thr Phe Leu Asn Trp Leu Leu Gln Arg Pro Gly Gln Ser
        35                  40                  45              
Pro Lys Arg Leu Ile Tyr Leu Val Ser Lys Leu Asp Ser Gly Val Pro
    50                  55                  60                  
Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile
65                  70                  75                  80  
Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Tyr Cys Trp Gln Gly
                85                  90                  95      
Thr His Phe Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys
            100                 105                 110         
Arg
    

