                               SEQUENCE LISTING

<110> TEMPO BIOSCIENCE, INC.
 
<120> BIOSENSORS FOR CHLORIDE IONS

<130> TEMPO-CHLORO

<140> 15/949,694
<141> 2018-04-10

<150> 62/484,425
<151> 2017-04-12

<160> 11    

<170> PatentIn version 3.5

<210> 1
<211> 483
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 1
Met Ala Gly Cys Arg Gly Ser Leu Cys Cys Cys Cys Arg Trp Cys Cys 
1               5                   10                  15      


Cys Cys Gly Glu Arg Glu Thr Arg Thr Pro Glu Glu Leu Thr Ile Leu 
            20                  25                  30          


Gly Glu Thr Gln Glu Glu Glu Asp Glu Ile Leu Pro Arg Lys Asp Tyr 
        35                  40                  45              


Glu Ser Leu Asp Tyr Asp Arg Cys Ile Asn Asp Pro Tyr Leu Glu Val 
    50                  55                  60                  


Leu Glu Thr Met Asp Asn Lys Lys Gly Arg Arg Tyr Glu Ala Val Lys 
65                  70                  75                  80  


Trp Met Val Val Phe Ala Ile Gly Val Cys Thr Gly Leu Pro Val Ala 
                85                  90                  95      


Ala Gly Ser Gly Ile Pro Glu Ile Lys Cys Tyr Leu Asn Gly Val Lys 
            100                 105                 110         


Val Pro Gly Ile Val Arg Leu Arg Thr Leu Leu Cys Lys Val Phe Gly 
        115                 120                 125             


Val Leu Phe Gly Val Ala Gly Gly Leu Phe Val Gly Lys Glu Gly Pro 
    130                 135                 140                 


Met Ile His Ser Gly Ala Val Val Gly Ala Gly Leu Pro Gln Phe Gln 
145                 150                 155                 160 


Ser Ile Ser Leu Arg Lys Ile Gln Phe Asn Phe Pro Tyr Phe Arg Ser 
                165                 170                 175     


Asp Arg Asp Lys Arg Asp Phe Val Ser Ala Gly Ala Ala Ala Gly Val 
            180                 185                 190         


Ala Ala Ala Phe Gly Ala Pro Ile Gly Gly Thr Leu Phe Ser Leu Glu 
        195                 200                 205             


Glu Gly Ser Ser Phe Trp Asn Gln Gly Leu Thr Trp Lys Val Leu Phe 
    210                 215                 220                 


Cys Ser Met Ser Ala Thr Phe Thr Leu Asn Phe Phe Arg Ser Gly Ile 
225                 230                 235                 240 


Gln Phe Gly Ser Trp Gly Ser Phe Gln Leu Pro Gly Leu Leu Asn Phe 
                245                 250                 255     


Gly Glu Phe Lys Cys Ser Asp Ser Asp Lys Lys Cys His Leu Trp Thr 
            260                 265                 270         


Ala Met Asp Leu Gly Phe Phe Val Met Met Gly Val Ile Gly Gly Leu 
        275                 280                 285             


Leu Gly Ala Thr Phe Asn Cys Leu Asn Lys Arg Leu Ala Lys Tyr Arg 
    290                 295                 300                 


Met Arg Asn Val His Pro Lys Pro Lys Leu Val Arg Val Leu Glu Ser 
305                 310                 315                 320 


Leu Leu Val Ser Leu Val Thr Thr Val Val Val Phe Val Ala Ser Met 
                325                 330                 335     


Val Leu Gly Glu Cys Arg Gln Met Ser Ser Ser Ser Gln Ile Ser Asn 
            340                 345                 350         


Gly Ser Phe Gln Leu Gln Val Thr Ser Glu Asp Val Asn Ser Ser Ile 
        355                 360                 365             


Lys Thr Phe Phe Cys Pro Asn Asp Thr Tyr Asn Asp Met Ala Thr Leu 
    370                 375                 380                 


Phe Phe Asn Ser Gln Glu Ser Ala Ile Leu Gln Leu Phe His Gln Asp 
385                 390                 395                 400 


Ala Thr Phe Leu Val Cys Ser Phe Thr Ser Tyr Ile Gly Leu Gly His 
                405                 410                 415     


Ile Tyr Ser Gly Thr Phe Ala Leu Ile Gly Ala Ala Ala Phe Leu Gly 
            420                 425                 430         


Gly Val Val Arg Met Thr Ile Ser Leu Thr Ile Ile Leu Ile Glu Ser 
        435                 440                 445             


Thr Asn Glu Ile Thr Tyr Gly Leu Pro Ile Met Val Thr Leu Met Val 
    450                 455                 460                 


Ala Lys Trp Thr Gly Asp Phe Phe Asn Lys Gly Ile Tyr Asp Ile His 
465                 470                 475                 480 


Val Gly Leu 
            


<210> 2
<211> 1449
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 2
atggcgggct gccgcggcag cctgtgctgc tgctgccgct ggtgctgctg ctgcggcgaa       60

cgcgaaaccc gcaccccgga agaactgacc attctgggcg aaacccagga agaagaagat      120

gaaattctgc cgcgcaaaga ttatgaaagc ctggattatg atcgctgcat taacgatccg      180

tatctggaag tgctggaaac catggataac aaaaaaggcc gccgctatga agcggtgaaa      240

tggatggtgg tgtttgcgat tggcgtgtgc accggcctgc cggtggcggc gggcagcggc      300

attccggaaa ttaaatgcta tctgaacggc gtgaaagtgc cgggcattgt gcgcctgcgc      360

accctgctgt gcaaagtgtt tggcgtgctg tttggcgtgg cgggcggcct gtttgtgggc      420

aaagaaggcc cgatgattca tagcggcgcg gtggtgggcg cgggcctgcc gcagtttcag      480

agcattagcc tgcgcaaaat tcagtttaac tttccgtatt ttcgcagcga tcgcgataaa      540

cgcgattttg tgagcgcggg cgcggcggcg ggcgtggcgg cggcgtttgg cgcgccgatt      600

ggcggcaccc tgtttagcct ggaagaaggc agcagctttt ggaaccaggg cctgacctgg      660

aaagtgctgt tttgcagcat gagcgcgacc tttaccctga acttttttcg cagcggcatt      720

cagtttggca gctggggcag ctttcagctg ccgggcctgc tgaactttgg cgaatttaaa      780

tgcagcgata gcgataaaaa atgccatctg tggaccgcga tggatctggg cttttttgtg      840

atgatgggcg tgattggcgg cctgctgggc gcgaccttta actgcctgaa caaacgcctg      900

gcgaaatatc gcatgcgcaa cgtgcatccg aaaccgaaac tggtgcgcgt gctggaaagc      960

ctgctggtga gcctggtgac caccgtggtg gtgtttgtgg cgagcatggt gctgggcgaa     1020

tgccgccaga tgagcagcag cagccagatt agcaacggca gctttcagct gcaggtgacc     1080

agcgaagatg tgaacagcag cattaaaacc tttttttgcc cgaacgatac ctataacgat     1140

atggcgaccc tgttttttaa cagccaggaa agcgcgattc tgcagctgtt tcatcaggat     1200

gcgacctttc tggtgtgcag ctttaccagc tatattggcc tgggccatat ttatagcggc     1260

acctttgcgc tgattggcgc ggcggcgttt ctgggcggcg tggtgcgcat gaccattagc     1320

ctgaccatta ttctgattga aagcaccaac gaaattacct atggcctgcc gattatggtg     1380

accctgatgg tggcgaaatg gaccggcgat ttttttaaca aaggcattta tgatattcat     1440

gtgggcctg                                                             1449


<210> 3
<211> 316
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 3
Met Tyr Thr Asp Cys Lys Glu Phe Ser Asn Thr Tyr His Asn Leu Gly 
1               5                   10                  15      


Arg Leu Pro Asp Glu Asp Arg His Trp Ser Ala Ser Cys His His His 
            20                  25                  30          


His His His Met Gly Met Ser Gly Ser Ser Met Ser Val Ile Lys Ser 
        35                  40                  45              


His Asn Val Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val 
    50                  55                  60                  


Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser 
65                  70                  75                  80  


Val Arg Gly Glu Gly Glu Gly Asp Ala Thr Asn Gly Lys Leu Thr Leu 
                85                  90                  95      


Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu 
            100                 105                 110         


Val Thr Thr Phe Gly Tyr Gly Val Ala Cys Phe Ser Arg Tyr Pro Asp 
        115                 120                 125             


His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr 
    130                 135                 140                 


Val Gln Glu Arg Thr Ile Ser Phe Lys Asp Asp Gly Thr Tyr Lys Thr 
145                 150                 155                 160 


Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu 
                165                 170                 175     


Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys 
            180                 185                 190         


Leu Glu Tyr Asn Phe Asn Ser His Tyr Val Tyr Ile Thr Ala Asp Lys 
        195                 200                 205             


Gln Lys Asn Ser Ile Lys Ala Asn Phe Lys Ile Arg His Asn Val Glu 
    210                 215                 220                 


Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile 
225                 230                 235                 240 


Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser His Gln 
                245                 250                 255     


Ser Lys Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu 
            260                 265                 270         


Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu 
        275                 280                 285             


Tyr Lys Ser Gly Leu Arg Ser Arg Ala Gln Ala Ser Asn Ser Ala Val 
    290                 295                 300                 


Asp Gly Thr Ala Gly Pro Gly Ser Thr Gly Ser Arg 
305                 310                 315     


<210> 4
<211> 951
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 4
atgtatacag actgtaagga attctctaat acatatcaca atctggggag acttcctgat       60

gaggacaggc actggagcgc ctcctgccat catcatcatc accatatggg gatgtctggc      120

tcatctatga gcgtaatcaa gtcccacaat gtcatggtgt caaaagggga agagttgttt      180

accggggttg ttcctattct cgtagagttg gacggtgacg tgaatggtca caagttctcc      240

gttcgcggcg aaggagaggg tgatgcaacc aatgggaaac tgaccctgaa attcatatgc      300

acgactggta aacttcctgt accgtggccg acactcgtta ccacgttcgg gtacggagtg      360

gcttgcttct ccagataccc cgaccacatg aaacaacacg actttttcaa atctgcaatg      420

cccgagggct atgttcagga acggaccata tcttttaagg atgatggaac atataaaaca      480

agggcagaag taaaatttga aggggatact ttggtgaaca ggatagagct caaagggata      540

gattttaagg aggacggaaa tatactgggc cataagcttg agtataattt caactcacac      600

tacgtctata ttacagctga caaacaaaag aacagtatca aagcgaactt caagattcgc      660

cataatgtcg aagatggaag cgtgcaactc gccgaccatt atcagcagaa cactccgata      720

ggcgatggac cagtacttct gcccgacaac cactatttga gtcaccagag taagttgagt      780

aaggacccta acgagaagag agatcacatg gtcttgctcg aatttgtgac tgcagcgggt      840

attaccttgg gaatggacga gctttacaag tctggtctta gatcacgagc acaggcttca      900

aattctgcag tagatggtac agccggaccc gggagcacag gttctaggta a               951


<210> 5
<211> 8
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 5
Asp Pro Lys Lys Lys Arg Lys Val 
1               5               


<210> 6
<211> 4
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 6
Lys Asp Glu Leu 
1               


<210> 7
<211> 14
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (6)..(7)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (11)..(12)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (13)..(14)
<223> Any residue with a hydrophobic side group

<400> 7
Asn Pro Thr Tyr Asp Xaa Xaa Leu Leu Tyr Xaa Xaa Xaa Xaa 
1               5                   10                  


<210> 8
<211> 20
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (2)..(2)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (4)..(4)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (7)..(7)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (17)..(17)
<223> Any amino acid

<400> 8
Val Xaa Pro Xaa Arg Val Xaa Pro Lys Val His Pro Ser Ser Thr Ala 
1               5                   10                  15      


Xaa Glu Gly Gly 
            20  


<210> 9
<211> 31
<212> PRT
<213> Homo sapiens

<400> 9
Met Thr Ser Arg Lys Lys Val Leu Leu Lys Val Ile Ile Leu Gly Asp 
1               5                   10                  15      


Ser Gly Val Gly Lys Thr Ser Leu Met His Arg Tyr Val Asn Asp 
            20                  25                  30      


<210> 10
<211> 571
<212> DNA
<213> Homo sapiens

<400> 10
cctgcagggc ccactagtat ctgcagaggg ccctgcgtat gagtgcaagt gggttttagg       60

accaggatga ggcggggtgg gggtgcctac ctgacgaccg accccgaccc actggacaag      120

cacccaaccc ccattcccca aattgcgcat cccctatcag agagggggag gggaaacagg      180

atgcggcgag gcgcgtgcgc actgccagct tcagcaccgc ggacagtgcc ttcgcccccg      240

cctggcggcg cgcgccaccg ccgcctcagc actgaaggcg cgctgacgtc actcgccggt      300

cccccgcaaa ctccccttcc cggccacctt ggtcgcgtcc gcgccgccgc cggcccagcc      360

ggaccgcacc acgcgaggcg cgagataggg gggcacgggc gcgaccatct gcgctgcggc      420

gccggcgact cagcgctgcc tcagtctgcg gtgggcagcg gaggagtcgt gtcgtgcctg      480

agagcgcagc tgtgctcctg ggcaccgcgc agtccgcccc cgcggctcct ggccagacca      540

cccctaggac cccctgcccc aagtcgcagc c                                     571


<210> 11
<211> 190
<212> PRT
<213> Homo sapiens

<400> 11
Met Asp Cys Leu Cys Ile Val Thr Thr Lys Lys Tyr Arg Tyr Gln Asp 
1               5                   10                  15      


Glu Asp Thr Pro Pro Leu Glu His Ser Pro Ala His Leu Pro Asn Gln 
            20                  25                  30          


Ala Asn Ser Pro Pro Val Ile Val Asn Thr Asp Thr Leu Glu Ala Pro 
        35                  40                  45              


Gly Tyr Glu Leu Gln Val Asn Gly Thr Glu Gly Glu Met Glu Tyr Glu 
    50                  55                  60                  


Glu Ile Thr Leu Glu Arg Gly Asn Ser Gly Leu Gly Phe Ser Ile Ala 
65                  70                  75                  80  


Gly Gly Thr Asp Asn Pro His Ile Gly Asp Asp Pro Ser Ile Phe Ile 
                85                  90                  95      


Thr Lys Ile Ile Pro Gly Gly Ala Ala Ala Gln Asp Gly Arg Leu Arg 
            100                 105                 110         


Val Asn Asp Ser Ile Leu Phe Val Asn Glu Val Asp Val Arg Glu Val 
        115                 120                 125             


Thr His Ser Ala Ala Val Glu Ala Leu Lys Glu Ala Gly Ser Ile Val 
    130                 135                 140                 


Arg Leu Tyr Val Met Arg Arg Lys Pro Pro Ala Glu Lys Val Met Glu 
145                 150                 155                 160 


Ile Lys Leu Ile Lys Gly Pro Lys Gly Leu Gly Phe Ser Ile Ala Gly 
                165                 170                 175     


Gly Val Gly Asn Ser Thr Ser Leu Glu Ile Thr Ala Ser Met 
            180                 185                 190 


