                         SEQUENCE LISTING

<110>  The Regents of the University of California
 
<120>  GENERATION OF HAPLOID PLANTS

<130>  081906-1081477-221420PC

<140>  PCT/US18/25254
<141>  2018-03-29

<150>  US 62/483,750
<151>  2017-04-10

<160>  55    

<170>  PatentIn version 3.5

<210>  1
<211>  1149
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic TEV protease DNA sequence for yeast:P14-myc-TEV234-stop

<400>  1
atggcgatgc aagcggccaa gagggcgaac attcgtcttc cacctgaagt aaatagaata       60

ttgtatataa gaaatttgcc atacaaaatc acagctgaag aaatgtatga tatatttggg      120

aaatatggac ctattcgtca aatcagagtg gggaacacac ctgaaactag aggaacagct      180

tatgtggtct atgaggacat ctttgatgcc aagaatgcat gtgatcacct atcgggattc      240

aatgtttgta acagatacct tgtggttttg tactataatg ccaacagggc atttcagaag      300

atggacacaa agaagaagga ggaacagttg aagcttttga aggagaaata tggcatcaac      360

acatatcctc ccaagatgga acaaaagttg atatctgaag aagacttgcc catgagcggc      420

ctggtgccga gaggcagcgc catgggagaa agcttgttta agggaccacg tgattacaac      480

ccgatatcga gcgacatttg tcatttgacg aatgaatctg atgggcacac aacatcgttg      540

tatggtattg gatttggtcc cttcatcatt acaaacaagc acttgtttcg tagaaataat      600

ggaacactgt tggtccaatc actacatggt gtattcaagg tcaaggacac cacgactttg      660

caacaacact tggttgatgg gagggacatg atgattatta gaatgcctaa ggatttccca      720

ccatttcctc aaaagctgaa atttagagag ccacaaaggg aagagagaat atgtcttgtg      780

acaaccaact tccaagctaa gagcatgtct agcatggtgt cagacactag ttgcacattc      840

ccttcatctg atggtatatt ctggaagcat tggattcaaa ccaaggatgg gcagtgtggc      900

agtccattag tatcaactag agatgggttc attgttggta tacactcagc atcgaatttc      960

accaacacaa acaattattt cacaagcgtg ccgaaaaact tcatggaatt gttgacaaat     1020

caggaggcgc agcagtgggt tagtggttgg agattaaacg ctgactcagt attgtggggg     1080

ggccataaag ttttcatggt taaacctgaa gaaccttttc agccagttaa ggaagcgact     1140

caattgtaa                                                             1149


<210>  2
<211>  375
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic P14 DNA sequence

<400>  2
atggcgatgc aagcggccaa gagggcgaac attcgtcttc cacctgaagt aaatagaata       60

ttgtatataa gaaatttgcc atacaaaatc acagctgaag aaatgtatga tatatttggg      120

aaatatggac ctattcgtca aatcagagtg gggaacacac ctgaaactag aggaacagct      180

tatgtggtct atgaggacat ctttgatgcc aagaatgcat gtgatcacct atcgggattc      240

aatgtttgta acagatacct tgtggttttg tactataatg ccaacagggc atttcagaag      300

atggacacaa agaagaagga ggaacagttg aagcttttga aggagaaata tggcatcaac      360

acatatcctc ccaag                                                       375


<210>  3
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic myc DNA sequence

<400>  3
atggaacaaa agttgatatc tgaagaagac ttgcccatga gcggcctggt gccgagaggc       60

agcgcc                                                                  66


<210>  4
<211>  705
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic TEV234 DNA sequence

<400>  4
atgggagaaa gcttgtttaa gggaccacgt gattacaacc cgatatcgag cgacatttgt       60

catttgacga atgaatctga tgggcacaca acatcgttgt atggtattgg atttggtccc      120

ttcatcatta caaacaagca cttgtttcgt agaaataatg gaacactgtt ggtccaatca      180

ctacatggtg tattcaaggt caaggacacc acgactttgc aacaacactt ggttgatggg      240

agggacatga tgattattag aatgcctaag gatttcccac catttcctca aaagctgaaa      300

tttagagagc cacaaaggga agagagaata tgtcttgtga caaccaactt ccaagctaag      360

agcatgtcta gcatggtgtc agacactagt tgcacattcc cttcatctga tggtatattc      420

tggaagcatt ggattcaaac caaggatggg cagtgtggca gtccattagt atcaactaga      480

gatgggttca ttgttggtat acactcagca tcgaatttca ccaacacaaa caattatttc      540

acaagcgtgc cgaaaaactt catggaattg ttgacaaatc aggaggcgca gcagtgggtt      600

agtggttgga gattaaacgc tgactcagta ttgtgggggg gccataaagt tttcatggtt      660

aaacctgaag aaccttttca gccagttaag gaagcgactc aattg                      705


<210>  5
<211>  382
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic TEV protease protein sequence for 
       yeast:P14-myc-TEV234-stop

<400>  5

Met Ala Met Gln Ala Ala Lys Arg Ala Asn Ile Arg Leu Pro Pro Glu 
1               5                   10                  15      


Val Asn Arg Ile Leu Tyr Ile Arg Asn Leu Pro Tyr Lys Ile Thr Ala 
            20                  25                  30          


Glu Glu Met Tyr Asp Ile Phe Gly Lys Tyr Gly Pro Ile Arg Gln Ile 
        35                  40                  45              


Arg Val Gly Asn Thr Pro Glu Thr Arg Gly Thr Ala Tyr Val Val Tyr 
    50                  55                  60                  


Glu Asp Ile Phe Asp Ala Lys Asn Ala Cys Asp His Leu Ser Gly Phe 
65                  70                  75                  80  


Asn Val Cys Asn Arg Tyr Leu Val Val Leu Tyr Tyr Asn Ala Asn Arg 
                85                  90                  95      


Ala Phe Gln Lys Met Asp Thr Lys Lys Lys Glu Glu Gln Leu Lys Leu 
            100                 105                 110         


Leu Lys Glu Lys Tyr Gly Ile Asn Thr Tyr Pro Pro Lys Met Glu Gln 
        115                 120                 125             


Lys Leu Ile Ser Glu Glu Asp Leu Pro Met Ser Gly Leu Val Pro Arg 
    130                 135                 140                 


Gly Ser Ala Met Gly Glu Ser Leu Phe Lys Gly Pro Arg Asp Tyr Asn 
145                 150                 155                 160 


Pro Ile Ser Ser Asp Ile Cys His Leu Thr Asn Glu Ser Asp Gly His 
                165                 170                 175     


Thr Thr Ser Leu Tyr Gly Ile Gly Phe Gly Pro Phe Ile Ile Thr Asn 
            180                 185                 190         


Lys His Leu Phe Arg Arg Asn Asn Gly Thr Leu Leu Val Gln Ser Leu 
        195                 200                 205             


His Gly Val Phe Lys Val Lys Asp Thr Thr Thr Leu Gln Gln His Leu 
    210                 215                 220                 


Val Asp Gly Arg Asp Met Met Ile Ile Arg Met Pro Lys Asp Phe Pro 
225                 230                 235                 240 


Pro Phe Pro Gln Lys Leu Lys Phe Arg Glu Pro Gln Arg Glu Glu Arg 
                245                 250                 255     


Ile Cys Leu Val Thr Thr Asn Phe Gln Ala Lys Ser Met Ser Ser Met 
            260                 265                 270         


Val Ser Asp Thr Ser Cys Thr Phe Pro Ser Ser Asp Gly Ile Phe Trp 
        275                 280                 285             


Lys His Trp Ile Gln Thr Lys Asp Gly Gln Cys Gly Ser Pro Leu Val 
    290                 295                 300                 


Ser Thr Arg Asp Gly Phe Ile Val Gly Ile His Ser Ala Ser Asn Phe 
305                 310                 315                 320 


Thr Asn Thr Asn Asn Tyr Phe Thr Ser Val Pro Lys Asn Phe Met Glu 
                325                 330                 335     


Leu Leu Thr Asn Gln Glu Ala Gln Gln Trp Val Ser Gly Trp Arg Leu 
            340                 345                 350         


Asn Ala Asp Ser Val Leu Trp Gly Gly His Lys Val Phe Met Val Lys 
        355                 360                 365             


Pro Glu Glu Pro Phe Gln Pro Val Lys Glu Ala Thr Gln Leu 
    370                 375                 380         


<210>  6
<211>  125
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic P14 protein sequence

<400>  6

Met Ala Met Gln Ala Ala Lys Arg Ala Asn Ile Arg Leu Pro Pro Glu 
1               5                   10                  15      


Val Asn Arg Ile Leu Tyr Ile Arg Asn Leu Pro Tyr Lys Ile Thr Ala 
            20                  25                  30          


Glu Glu Met Tyr Asp Ile Phe Gly Lys Tyr Gly Pro Ile Arg Gln Ile 
        35                  40                  45              


Arg Val Gly Asn Thr Pro Glu Thr Arg Gly Thr Ala Tyr Val Val Tyr 
    50                  55                  60                  


Glu Asp Ile Phe Asp Ala Lys Asn Ala Cys Asp His Leu Ser Gly Phe 
65                  70                  75                  80  


Asn Val Cys Asn Arg Tyr Leu Val Val Leu Tyr Tyr Asn Ala Asn Arg 
                85                  90                  95      


Ala Phe Gln Lys Met Asp Thr Lys Lys Lys Glu Glu Gln Leu Lys Leu 
            100                 105                 110         


Leu Lys Glu Lys Tyr Gly Ile Asn Thr Tyr Pro Pro Lys 
        115                 120                 125 


<210>  7
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic myc protein sequence

<400>  7

Met Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Pro Met Ser Gly Leu 
1               5                   10                  15      


Val Pro Arg Gly Ser Ala 
            20          


<210>  8
<211>  235
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic TEV234 protein sequence

<400>  8

Met Gly Glu Ser Leu Phe Lys Gly Pro Arg Asp Tyr Asn Pro Ile Ser 
1               5                   10                  15      


Ser Asp Ile Cys His Leu Thr Asn Glu Ser Asp Gly His Thr Thr Ser 
            20                  25                  30          


Leu Tyr Gly Ile Gly Phe Gly Pro Phe Ile Ile Thr Asn Lys His Leu 
        35                  40                  45              


Phe Arg Arg Asn Asn Gly Thr Leu Leu Val Gln Ser Leu His Gly Val 
    50                  55                  60                  


Phe Lys Val Lys Asp Thr Thr Thr Leu Gln Gln His Leu Val Asp Gly 
65                  70                  75                  80  


Arg Asp Met Met Ile Ile Arg Met Pro Lys Asp Phe Pro Pro Phe Pro 
                85                  90                  95      


Gln Lys Leu Lys Phe Arg Glu Pro Gln Arg Glu Glu Arg Ile Cys Leu 
            100                 105                 110         


Val Thr Thr Asn Phe Gln Ala Lys Ser Met Ser Ser Met Val Ser Asp 
        115                 120                 125             


Thr Ser Cys Thr Phe Pro Ser Ser Asp Gly Ile Phe Trp Lys His Trp 
    130                 135                 140                 


Ile Gln Thr Lys Asp Gly Gln Cys Gly Ser Pro Leu Val Ser Thr Arg 
145                 150                 155                 160 


Asp Gly Phe Ile Val Gly Ile His Ser Ala Ser Asn Phe Thr Asn Thr 
                165                 170                 175     


Asn Asn Tyr Phe Thr Ser Val Pro Lys Asn Phe Met Glu Leu Leu Thr 
            180                 185                 190         


Asn Gln Glu Ala Gln Gln Trp Val Ser Gly Trp Arg Leu Asn Ala Asp 
        195                 200                 205             


Ser Val Leu Trp Gly Gly His Lys Val Phe Met Val Lys Pro Glu Glu 
    210                 215                 220                 


Pro Phe Gln Pro Val Lys Glu Ala Thr Gln Leu 
225                 230                 235 


<210>  9
<211>  1758
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic Degron construct DNA sequence for yeast:GFP cODC1 
       spacer TEVrec Ndegron spacer SF3b spacer mKATE stop

<400>  9
atgtctaaag gtgaagaatt attcactggt gttgtcccaa ttttggttga attagatggt       60

gatgttaatg gtcacaaatt ttctgtctcc ggtgaaggtg aaggtgatgc tacttacggt      120

aaattgacct taaaatttat ttgtactact ggtaaattgc cagttccatg gccaacctta      180

gtcactactt tcggttatgg tgttcaatgt tttgcgagat acccagatca tatgaaacaa      240

catgactttt tcaagtctgc catgccagaa ggttatgttc aagaaagaac tatttttttc      300

aaagatgacg gtaactacaa gaccagagct gaagtcaagt ttgaaggtga taccttagtt      360

aatagaatcg aattaaaagg tattgatttt aaagaagatg gtaacatttt aggtcacaaa      420

ttggaataca actataactc tcacaatgtt tacatcatgg ctgacaaaca aaagaatggt      480

atcaaagtta acttcaaaat tagacacaac attgaagatg gttctgttca attagctgac      540

cattatcaac aaaatactcc aattggtgat ggtccagtct tgttaccaga caaccattac      600

ttatccactc aatctgcctt atccaaagat ccaaacgaaa agagagacca catggtcttg      660

ttagaatttg ttactgctgc tggtattacc catggtatgg atgaattgta caaattgcca      720

atgtcttgtg cacaagaatc tattacttct ttgtacaaga aggctggttc tgaaaacttg      780

tacttccaat tccacaagtc tggtgcttgg aagttgccag tttctttggt taagagaggg      840

atcgataagc ttgattataa agaacagctt caggcttggc ggtgggaaag agaaattgat      900

gagagaaatc gcccactttc tgatgaggaa ttagatgcta tgttcccaga aggatataag      960

gtacttcctc ctccagctgg ttatgttcct attcgaactc cagctcatat ggatcgaatt     1020

cctgcagtag caggtgctgg tgctggtgct ggagcaatgt ctgaattaat taaagaaaat     1080

atgcatatga aattatatat ggaaggtaca gttaataatc atcattttaa atgtacatct     1140

gaaggtgaag gtaaaccata tgaaggtaca caaacaatga gaattaaagt tgttgaaggt     1200

ggtccattac catttgcttt tgatatttta gctacatctt ttatgtatgg ttctaagaca     1260

tttattaatc atacacaagg tattccagat ttttttaaac aatcttttcc agaaggtttt     1320

acatgggaaa gagttacaac atatgaagat ggtggtgttt taacagctac acaagataca     1380

tctttacaag atggttgttt aatttataat gttaaaatta gaggtgttaa ttttccatct     1440

aatggtccag ttatgcaaaa aaaaacatta ggttgggaag cttctacaga aatgttatat     1500

ccagctgatg gtggtttaga aggtaggtct gatatggctt taaaattagt tggtggtggt     1560

catttaattt gtaatttgaa aacaacatat aggtctaaaa aaccagctaa aaatttaaaa     1620

atgccaggtg tttattatgt tgatagaaga ttagaaagaa ttaaagaagc tgataaagaa     1680

acatatgttg aacaacatga agttgctgtt gctagatatt gtgatttacc atctaaatta     1740

ggtcataaag gatcctaa                                                   1758


<210>  10
<211>  714
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic GFP DNA sequence

<400>  10
atgtctaaag gtgaagaatt attcactggt gttgtcccaa ttttggttga attagatggt       60

gatgttaatg gtcacaaatt ttctgtctcc ggtgaaggtg aaggtgatgc tacttacggt      120

aaattgacct taaaatttat ttgtactact ggtaaattgc cagttccatg gccaacctta      180

gtcactactt tcggttatgg tgttcaatgt tttgcgagat acccagatca tatgaaacaa      240

catgactttt tcaagtctgc catgccagaa ggttatgttc aagaaagaac tatttttttc      300

aaagatgacg gtaactacaa gaccagagct gaagtcaagt ttgaaggtga taccttagtt      360

aatagaatcg aattaaaagg tattgatttt aaagaagatg gtaacatttt aggtcacaaa      420

ttggaataca actataactc tcacaatgtt tacatcatgg ctgacaaaca aaagaatggt      480

atcaaagtta acttcaaaat tagacacaac attgaagatg gttctgttca attagctgac      540

cattatcaac aaaatactcc aattggtgat ggtccagtct tgttaccaga caaccattac      600

ttatccactc aatctgcctt atccaaagat ccaaacgaaa agagagacca catggtcttg      660

ttagaatttg ttactgctgc tggtattacc catggtatgg atgaattgta caaa            714


<210>  11
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic cODC1 DNA sequence

<400>  11
ttgccaatgt cttgtgcaca agaa                                              24


<210>  12
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic spacer DNA sequence

<400>  12
tctattactt ctttgtacaa gaaggctggt tct                                    33


<210>  13
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic TEVrec DNA sequence

<400>  13
gaaaacttgt acttccaatt c                                                 21


<210>  14
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic Ndegron DNA sequence

<400>  14
cacaagtctg gtgcttggaa gttgccagtt tctttggtta ag                          42


<210>  15
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic spacer DNA sequence

<400>  15
agagggatcg ataagcttga ttataaa                                           27


<210>  16
<211>  144
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic SF3b DNA sequence

<400>  16
gaacagcttc aggcttggcg gtgggaaaga gaaattgatg agagaaatcg cccactttct       60

gatgaggaat tagatgctat gttcccagaa ggatataagg tacttcctcc tccagctggt      120

tatgttccta ttcgaactcc agct                                             144


<210>  17
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic spacer DNA sequence

<400>  17
catatggatc gaattcctgc agta                                              24


<210>  18
<211>  726
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic TEV234 DNA sequence

<400>  18
gcaggtgctg gtgctggtgc tggagcaatg tctgaattaa ttaaagaaaa tatgcatatg       60

aaattatata tggaaggtac agttaataat catcatttta aatgtacatc tgaaggtgaa      120

ggtaaaccat atgaaggtac acaaacaatg agaattaaag ttgttgaagg tggtccatta      180

ccatttgctt ttgatatttt agctacatct tttatgtatg gttctaagac atttattaat      240

catacacaag gtattccaga tttttttaaa caatcttttc cagaaggttt tacatgggaa      300

agagttacaa catatgaaga tggtggtgtt ttaacagcta cacaagatac atctttacaa      360

gatggttgtt taatttataa tgttaaaatt agaggtgtta attttccatc taatggtcca      420

gttatgcaaa aaaaaacatt aggttgggaa gcttctacag aaatgttata tccagctgat      480

ggtggtttag aaggtaggtc tgatatggct ttaaaattag ttggtggtgg tcatttaatt      540

tgtaatttga aaacaacata taggtctaaa aaaccagcta aaaatttaaa aatgccaggt      600

gtttattatg ttgatagaag attagaaaga attaaagaag ctgataaaga aacatatgtt      660

gaacaacatg aagttgctgt tgctagatat tgtgatttac catctaaatt aggtcataaa      720

ggatcc                                                                 726


<210>  19
<211>  585
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic Degron protein DNA sequence for yeast:GFP cODC1 spacer 
       TEVrec Ndegron spacer SF3b spacer mKATE stop

<400>  19

Met Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe 
    50                  55                  60                  


Gly Tyr Gly Val Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
    210                 215                 220                 


Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys Leu Pro 
225                 230                 235                 240 


Met Ser Cys Ala Gln Glu Ser Ile Thr Ser Leu Tyr Lys Lys Ala Gly 
                245                 250                 255     


Ser Glu Asn Leu Tyr Phe Gln Phe His Lys Ser Gly Ala Trp Lys Leu 
            260                 265                 270         


Pro Val Ser Leu Val Lys Arg Gly Ile Asp Lys Leu Asp Tyr Lys Glu 
        275                 280                 285             


Gln Leu Gln Ala Trp Arg Trp Glu Arg Glu Ile Asp Glu Arg Asn Arg 
    290                 295                 300                 


Pro Leu Ser Asp Glu Glu Leu Asp Ala Met Phe Pro Glu Gly Tyr Lys 
305                 310                 315                 320 


Val Leu Pro Pro Pro Ala Gly Tyr Val Pro Ile Arg Thr Pro Ala His 
                325                 330                 335     


Met Asp Arg Ile Pro Ala Val Ala Gly Ala Gly Ala Gly Ala Gly Ala 
            340                 345                 350         


Met Ser Glu Leu Ile Lys Glu Asn Met His Met Lys Leu Tyr Met Glu 
        355                 360                 365             


Gly Thr Val Asn Asn His His Phe Lys Cys Thr Ser Glu Gly Glu Gly 
    370                 375                 380                 


Lys Pro Tyr Glu Gly Thr Gln Thr Met Arg Ile Lys Val Val Glu Gly 
385                 390                 395                 400 


Gly Pro Leu Pro Phe Ala Phe Asp Ile Leu Ala Thr Ser Phe Met Tyr 
                405                 410                 415     


Gly Ser Lys Thr Phe Ile Asn His Thr Gln Gly Ile Pro Asp Phe Phe 
            420                 425                 430         


Lys Gln Ser Phe Pro Glu Gly Phe Thr Trp Glu Arg Val Thr Thr Tyr 
        435                 440                 445             


Glu Asp Gly Gly Val Leu Thr Ala Thr Gln Asp Thr Ser Leu Gln Asp 
    450                 455                 460                 


Gly Cys Leu Ile Tyr Asn Val Lys Ile Arg Gly Val Asn Phe Pro Ser 
465                 470                 475                 480 


Asn Gly Pro Val Met Gln Lys Lys Thr Leu Gly Trp Glu Ala Ser Thr 
                485                 490                 495     


Glu Met Leu Tyr Pro Ala Asp Gly Gly Leu Glu Gly Arg Ser Asp Met 
            500                 505                 510         


Ala Leu Lys Leu Val Gly Gly Gly His Leu Ile Cys Asn Leu Lys Thr 
        515                 520                 525             


Thr Tyr Arg Ser Lys Lys Pro Ala Lys Asn Leu Lys Met Pro Gly Val 
    530                 535                 540                 


Tyr Tyr Val Asp Arg Arg Leu Glu Arg Ile Lys Glu Ala Asp Lys Glu 
545                 550                 555                 560 


Thr Tyr Val Glu Gln His Glu Val Ala Val Ala Arg Tyr Cys Asp Leu 
                565                 570                 575     


Pro Ser Lys Leu Gly His Lys Gly Ser 
            580                 585 


<210>  20
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic GFP protein sequence

<400>  20

Met Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe 
    50                  55                  60                  


Gly Tyr Gly Val Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
    210                 215                 220                 


Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235             


<210>  21
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic cODC1 protein sequence

<400>  21

Leu Pro Met Ser Cys Ala Gln Glu 
1               5               


<210>  22
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic spacer protein sequence

<400>  22

Ser Ile Thr Ser Leu Tyr Lys Lys Ala Gly Ser 
1               5                   10      


<210>  23
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic TEVrec protein sequence

<400>  23

Glu Asn Leu Tyr Phe Gln Phe 
1               5           


<210>  24
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic Ndegron protein sequence

<400>  24

His Lys Ser Gly Ala Trp Lys Leu Pro Val Ser Leu Val Lys 
1               5                   10                  


<210>  25
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic spacer protein sequence

<400>  25

Arg Gly Ile Asp Lys Leu Asp Tyr Lys 
1               5                   


<210>  26
<211>  48
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic SF3b protein sequence

<400>  26

Glu Gln Leu Gln Ala Trp Arg Trp Glu Arg Glu Ile Asp Glu Arg Asn 
1               5                   10                  15      


Arg Pro Leu Ser Asp Glu Glu Leu Asp Ala Met Phe Pro Glu Gly Tyr 
            20                  25                  30          


Lys Val Leu Pro Pro Pro Ala Gly Tyr Val Pro Ile Arg Thr Pro Ala 
        35                  40                  45              


<210>  27
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic spacer protein sequence

<400>  27

His Met Asp Arg Ile Pro Ala Val 
1               5               


<210>  28
<211>  242
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic mKATE protein sequence

<400>  28

Ala Gly Ala Gly Ala Gly Ala Gly Ala Met Ser Glu Leu Ile Lys Glu 
1               5                   10                  15      


Asn Met His Met Lys Leu Tyr Met Glu Gly Thr Val Asn Asn His His 
            20                  25                  30          


Phe Lys Cys Thr Ser Glu Gly Glu Gly Lys Pro Tyr Glu Gly Thr Gln 
        35                  40                  45              


Thr Met Arg Ile Lys Val Val Glu Gly Gly Pro Leu Pro Phe Ala Phe 
    50                  55                  60                  


Asp Ile Leu Ala Thr Ser Phe Met Tyr Gly Ser Lys Thr Phe Ile Asn 
65                  70                  75                  80  


His Thr Gln Gly Ile Pro Asp Phe Phe Lys Gln Ser Phe Pro Glu Gly 
                85                  90                  95      


Phe Thr Trp Glu Arg Val Thr Thr Tyr Glu Asp Gly Gly Val Leu Thr 
            100                 105                 110         


Ala Thr Gln Asp Thr Ser Leu Gln Asp Gly Cys Leu Ile Tyr Asn Val 
        115                 120                 125             


Lys Ile Arg Gly Val Asn Phe Pro Ser Asn Gly Pro Val Met Gln Lys 
    130                 135                 140                 


Lys Thr Leu Gly Trp Glu Ala Ser Thr Glu Met Leu Tyr Pro Ala Asp 
145                 150                 155                 160 


Gly Gly Leu Glu Gly Arg Ser Asp Met Ala Leu Lys Leu Val Gly Gly 
                165                 170                 175     


Gly His Leu Ile Cys Asn Leu Lys Thr Thr Tyr Arg Ser Lys Lys Pro 
            180                 185                 190         


Ala Lys Asn Leu Lys Met Pro Gly Val Tyr Tyr Val Asp Arg Arg Leu 
        195                 200                 205             


Glu Arg Ile Lys Glu Ala Asp Lys Glu Thr Tyr Val Glu Gln His Glu 
    210                 215                 220                 


Val Ala Val Ala Arg Tyr Cys Asp Leu Pro Ser Lys Leu Gly His Lys 
225                 230                 235                 240 


Gly Ser 
        


<210>  29
<211>  1428
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic tdTomato DNA sequence (Arabidopsis-optimized)

<400>  29
atggtgagca agggcgagga ggtcatcaaa gagttcatgc gcttcaaggt gcgcatggag       60

ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag      120

ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac      180

atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc      240

cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc      300

gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc      360

tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag      420

accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc      480

gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagacc      540

atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga caccaagctg      600

gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc      660

cgccaccacc tgttcctggg gcatggcacc ggcagcaccg gcagcggcag ctccggcacc      720

gcctcctccg aggacaacaa catggccgtc atcaaagagt tcatgcgctt caaggtgcgc      780

atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc      840

tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc      900

tgggacatcc tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc      960

gacatccccg attacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg     1020

aacttcgagg acggcggtct ggtgaccgtg acccaggact cctccctgca ggacggcacg     1080

ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag     1140

aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg     1200

aagggcgaga tccaccaggc cctgaagctg aaggacggcg gccactacct ggtggagttc     1260

aagaccatct acatggccaa gaagcccgtg caactgcccg gctactacta cgtggacacc     1320

aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc     1380

gagggccgcc accacctgtt cctgtacggc atggacgagc tgtacaag                  1428


<210>  30
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic spacer DNA sequence (Arabidopsis-optimized)

<400>  30
gccgctagtg cgatcgcatc agggagtggt tccggaagcg gctctggatc gggctcaggg       60

agtggttccg gcagcggctc tggatcggcg gccgctgca                              99


<210>  31
<211>  711
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  syntheticTEV DNA sequence (Arabidopsis-optimized)

<400>  31
atgggagaaa gcttgtttaa ggggccgcgt gattacaacc cgatatcgag caccatttgt       60

catttgacga atgaatctga tgggcacaca acatcgttgt atggtattgg atttggtccc      120

ttcatcatta caaacaagca cttgtttaga agaaataatg gaacactggt ggtccaatca      180

ctacatggtg tattcaaggt caagaacacc acgactttgc aacaacacct cattgatggg      240

agggacatga taattattcg catgcctaag gatttcccac catttcctca aaagctgaaa      300

tttagagagc cacaaaggga agagcgcata tgtcttgtga caaccaactt ccaaactaag      360

agcatgtcta gcatggtgtc agacactagt tgcacattcc cttcaggaga tggcatattc      420

tggaagcatt ggattcaaac caaggatggg cagtgtggca gtccattagt atcaactaga      480

gatgggttca ttgttggtat acactcagca tcgaatttca ccaacacaaa caattatttc      540

acaagcgtgc cgaaaaactt catggaattg ttgacaaatc aggaggcgca gcagtgggtt      600

agtggttggc gattaaatgc tgactcagta ttgtgggggg gccataaagt tttcatggtg      660

aaacctgaag agccttttca gccagttaag gaagcgactc aactcatgaa t               711


<210>  32
<211>  476
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic tdTomato protein sequence (Arabidopsis-optimized)

<400>  32

Met Val Ser Lys Gly Glu Glu Val Ile Lys Glu Phe Met Arg Phe Lys 
1               5                   10                  15      


Val Arg Met Glu Gly Ser Met Asn Gly His Glu Phe Glu Ile Glu Gly 
            20                  25                  30          


Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala Lys Leu Lys 
        35                  40                  45              


Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser Pro 
    50                  55                  60                  


Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His Pro Ala Asp Ile 
65                  70                  75                  80  


Pro Asp Tyr Lys Lys Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg 
                85                  90                  95      


Val Met Asn Phe Glu Asp Gly Gly Leu Val Thr Val Thr Gln Asp Ser 
            100                 105                 110         


Ser Leu Gln Asp Gly Thr Leu Ile Tyr Lys Val Lys Met Arg Gly Thr 
        115                 120                 125             


Asn Phe Pro Pro Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp 
    130                 135                 140                 


Glu Ala Ser Thr Glu Arg Leu Tyr Pro Arg Asp Gly Val Leu Lys Gly 
145                 150                 155                 160 


Glu Ile His Gln Ala Leu Lys Leu Lys Asp Gly Gly His Tyr Leu Val 
                165                 170                 175     


Glu Phe Lys Thr Ile Tyr Met Ala Lys Lys Pro Val Gln Leu Pro Gly 
            180                 185                 190         


Tyr Tyr Tyr Val Asp Thr Lys Leu Asp Ile Thr Ser His Asn Glu Asp 
        195                 200                 205             


Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ser Glu Gly Arg His His Leu 
    210                 215                 220                 


Phe Leu Gly His Gly Thr Gly Ser Thr Gly Ser Gly Ser Ser Gly Thr 
225                 230                 235                 240 


Ala Ser Ser Glu Asp Asn Asn Met Ala Val Ile Lys Glu Phe Met Arg 
                245                 250                 255     


Phe Lys Val Arg Met Glu Gly Ser Met Asn Gly His Glu Phe Glu Ile 
            260                 265                 270         


Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala Lys 
        275                 280                 285             


Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile Leu 
    290                 295                 300                 


Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His Pro Ala 
305                 310                 315                 320 


Asp Ile Pro Asp Tyr Lys Lys Leu Ser Phe Pro Glu Gly Phe Lys Trp 
                325                 330                 335     


Glu Arg Val Met Asn Phe Glu Asp Gly Gly Leu Val Thr Val Thr Gln 
            340                 345                 350         


Asp Ser Ser Leu Gln Asp Gly Thr Leu Ile Tyr Lys Val Lys Met Arg 
        355                 360                 365             


Gly Thr Asn Phe Pro Pro Asp Gly Pro Val Met Gln Lys Lys Thr Met 
    370                 375                 380                 


Gly Trp Glu Ala Ser Thr Glu Arg Leu Tyr Pro Arg Asp Gly Val Leu 
385                 390                 395                 400 


Lys Gly Glu Ile His Gln Ala Leu Lys Leu Lys Asp Gly Gly His Tyr 
                405                 410                 415     


Leu Val Glu Phe Lys Thr Ile Tyr Met Ala Lys Lys Pro Val Gln Leu 
            420                 425                 430         


Pro Gly Tyr Tyr Tyr Val Asp Thr Lys Leu Asp Ile Thr Ser His Asn 
        435                 440                 445             


Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ser Glu Gly Arg His 
    450                 455                 460                 


His Leu Phe Leu Tyr Gly Met Asp Glu Leu Tyr Lys 
465                 470                 475     


<210>  33
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic spacer protein sequence (Arabidopsis-optimized)

<400>  33

Ala Ala Ser Ala Ile Ala Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly 
1               5                   10                  15      


Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Ala Ala Ala 
            20                  25                  30          


Ala 
    


<210>  34
<211>  237
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic TEV protein sequence (Arabidopsis-optimized)

<400>  34

Met Gly Glu Ser Leu Phe Lys Gly Pro Arg Asp Tyr Asn Pro Ile Ser 
1               5                   10                  15      


Ser Thr Ile Cys His Leu Thr Asn Glu Ser Asp Gly His Thr Thr Ser 
            20                  25                  30          


Leu Tyr Gly Ile Gly Phe Gly Pro Phe Ile Ile Thr Asn Lys His Leu 
        35                  40                  45              


Phe Arg Arg Asn Asn Gly Thr Leu Val Val Gln Ser Leu His Gly Val 
    50                  55                  60                  


Phe Lys Val Lys Asn Thr Thr Thr Leu Gln Gln His Leu Ile Asp Gly 
65                  70                  75                  80  


Arg Asp Met Ile Ile Ile Arg Met Pro Lys Asp Phe Pro Pro Phe Pro 
                85                  90                  95      


Gln Lys Leu Lys Phe Arg Glu Pro Gln Arg Glu Glu Arg Ile Cys Leu 
            100                 105                 110         


Val Thr Thr Asn Phe Gln Thr Lys Ser Met Ser Ser Met Val Ser Asp 
        115                 120                 125             


Thr Ser Cys Thr Phe Pro Ser Gly Asp Gly Ile Phe Trp Lys His Trp 
    130                 135                 140                 


Ile Gln Thr Lys Asp Gly Gln Cys Gly Ser Pro Leu Val Ser Thr Arg 
145                 150                 155                 160 


Asp Gly Phe Ile Val Gly Ile His Ser Ala Ser Asn Phe Thr Asn Thr 
                165                 170                 175     


Asn Asn Tyr Phe Thr Ser Val Pro Lys Asn Phe Met Glu Leu Leu Thr 
            180                 185                 190         


Asn Gln Glu Ala Gln Gln Trp Val Ser Gly Trp Arg Leu Asn Ala Asp 
        195                 200                 205             


Ser Val Leu Trp Gly Gly His Lys Val Phe Met Val Lys Pro Glu Glu 
    210                 215                 220                 


Pro Phe Gln Pro Val Lys Glu Ala Thr Gln Leu Met Asn 
225                 230                 235         


<210>  35
<211>  1728
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic Degron DNA sequence for Arabidopsis:Citrine spacer 
       TEVsite Ndeg spacer WUS

<400>  35
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac       60

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac      120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc      180

ctcgtgacca ccttcggcta cggcctgatg tgcttcgccc gctaccccga ccacatgaag      240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc      300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg      360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac      420

aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac      480

ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc      540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac      600

tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc      660

ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggca      720

gcggccgctt cagggagtgg ttccggaagc gaaaacttgt acttccaatt ccacaagtct      780

ggtgcttgga agttgccagt ttctttggtt aaggggagtg gttccggcag cggctctgga      840

tcgtcaattg ctatggagcc gccacagcat cagcatcatc atcatcaagc cgaccaagaa      900

agcggcaaca acaacaacaa caagtccggc tctggtggtt acacgtgtcg ccagaccagc      960

acgaggtgga caccgacgac ggagcaaatc aaaatcctca aagaacttta ctacaacaat     1020

gcaatccggt caccaacagc cgatcagatc cagaagatca ctgcaaggct gagacagttc     1080

ggaaagattg agggcaagaa cgtcttttac tggttccaga accataaggc tcgtgagcgt     1140

cagaagaaga gattcaacgg aacaaacatg accacaccat cttcatcacc caactcggtt     1200

atgatggcgg ctaacgatca ttatcatcct ctacttcacc atcatcacgg tgttcccatg     1260

cagagacctg ctaattccgt caacgttaaa cttaaccaag accatcatct ctatcatcat     1320

aacaagccat atcccagctt caataacggg aatttaaatc atgcaagctc aggtactgaa     1380

tgtggtgttg ttaatgcttc taatggctac atgagtagcc atgtctatgg atctatggaa     1440

caagactgtt ctatgaatta caacaacgta ggtggaggat gggcaaacat ggatcatcat     1500

tactcatctg caccttacaa cttcttcgat agagcaaagc ctctgtttgg tctagaaggt     1560

catcaagaag aagaagaatg tggtggcgat gcttatctgg aacatcgacg tacgcttcct     1620

ctcttcccta tgcacggtga agatcacatc aacggtggta gtggcgccat ctggaagtat     1680

ggccaatcgg aagttcgccc ttgcgcttct cttgagctac gtctgaac                  1728


<210>  36
<211>  717
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic Citrine DNA sequence

<400>  36
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac       60

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac      120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc      180

ctcgtgacca ccttcggcta cggcctgatg tgcttcgccc gctaccccga ccacatgaag      240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc      300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg      360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac      420

aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac      480

ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc      540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac      600

tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc      660

ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaag         717


<210>  37
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic spacer DNA sequence

<400>  37
gcagcggccg cttcagggag tggttccgga agc                                    33


<210>  38
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic TEVsite DNA sequence

<400>  38
gaaaacttgt acttccaatt c                                                 21


<210>  39
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic Ndeg DNA sequence

<400>  39
cacaagtctg gtgcttggaa gttgccagtt tctttggtta ag                          42


<210>  40
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic spacer DNA sequence

<400>  40
gggagtggtt ccggcagcgg ctctggatcg tcaattgct                              39


<210>  41
<211>  876
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic WUS DNA sequence

<400>  41
atggagccgc cacagcatca gcatcatcat catcaagccg accaagaaag cggcaacaac       60

aacaacaaca agtccggctc tggtggttac acgtgtcgcc agaccagcac gaggtggaca      120

ccgacgacgg agcaaatcaa aatcctcaaa gaactttact acaacaatgc aatccggtca      180

ccaacagccg atcagatcca gaagatcact gcaaggctga gacagttcgg aaagattgag      240

ggcaagaacg tcttttactg gttccagaac cataaggctc gtgagcgtca gaagaagaga      300

ttcaacggaa caaacatgac cacaccatct tcatcaccca actcggttat gatggcggct      360

aacgatcatt atcatcctct acttcaccat catcacggtg ttcccatgca gagacctgct      420

aattccgtca acgttaaact taaccaagac catcatctct atcatcataa caagccatat      480

cccagcttca ataacgggaa tttaaatcat gcaagctcag gtactgaatg tggtgttgtt      540

aatgcttcta atggctacat gagtagccat gtctatggat ctatggaaca agactgttct      600

atgaattaca acaacgtagg tggaggatgg gcaaacatgg atcatcatta ctcatctgca      660

ccttacaact tcttcgatag agcaaagcct ctgtttggtc tagaaggtca tcaagaagaa      720

gaagaatgtg gtggcgatgc ttatctggaa catcgacgta cgcttcctct cttccctatg      780

cacggtgaag atcacatcaa cggtggtagt ggcgccatct ggaagtatgg ccaatcggaa      840

gttcgccctt gcgcttctct tgagctacgt ctgaac                                876


<210>  42
<211>  576
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic Degron protein sequence for Arabidopsis:Citrine spacer 
       TEVsite Ndeg spacer WUS

<400>  42

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Phe Gly Tyr Gly Leu Met Cys Phe Ala Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Ala Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Ala 
225                 230                 235                 240 


Ala Ala Ala Ser Gly Ser Gly Ser Gly Ser Glu Asn Leu Tyr Phe Gln 
                245                 250                 255     


Phe His Lys Ser Gly Ala Trp Lys Leu Pro Val Ser Leu Val Lys Gly 
            260                 265                 270         


Ser Gly Ser Gly Ser Gly Ser Gly Ser Ser Ile Ala Met Glu Pro Pro 
        275                 280                 285             


Gln His Gln His His His His Gln Ala Asp Gln Glu Ser Gly Asn Asn 
    290                 295                 300                 


Asn Asn Asn Lys Ser Gly Ser Gly Gly Tyr Thr Cys Arg Gln Thr Ser 
305                 310                 315                 320 


Thr Arg Trp Thr Pro Thr Thr Glu Gln Ile Lys Ile Leu Lys Glu Leu 
                325                 330                 335     


Tyr Tyr Asn Asn Ala Ile Arg Ser Pro Thr Ala Asp Gln Ile Gln Lys 
            340                 345                 350         


Ile Thr Ala Arg Leu Arg Gln Phe Gly Lys Ile Glu Gly Lys Asn Val 
        355                 360                 365             


Phe Tyr Trp Phe Gln Asn His Lys Ala Arg Glu Arg Gln Lys Lys Arg 
    370                 375                 380                 


Phe Asn Gly Thr Asn Met Thr Thr Pro Ser Ser Ser Pro Asn Ser Val 
385                 390                 395                 400 


Met Met Ala Ala Asn Asp His Tyr His Pro Leu Leu His His His His 
                405                 410                 415     


Gly Val Pro Met Gln Arg Pro Ala Asn Ser Val Asn Val Lys Leu Asn 
            420                 425                 430         


Gln Asp His His Leu Tyr His His Asn Lys Pro Tyr Pro Ser Phe Asn 
        435                 440                 445             


Asn Gly Asn Leu Asn His Ala Ser Ser Gly Thr Glu Cys Gly Val Val 
    450                 455                 460                 


Asn Ala Ser Asn Gly Tyr Met Ser Ser His Val Tyr Gly Ser Met Glu 
465                 470                 475                 480 


Gln Asp Cys Ser Met Asn Tyr Asn Asn Val Gly Gly Gly Trp Ala Asn 
                485                 490                 495     


Met Asp His His Tyr Ser Ser Ala Pro Tyr Asn Phe Phe Asp Arg Ala 
            500                 505                 510         


Lys Pro Leu Phe Gly Leu Glu Gly His Gln Glu Glu Glu Glu Cys Gly 
        515                 520                 525             


Gly Asp Ala Tyr Leu Glu His Arg Arg Thr Leu Pro Leu Phe Pro Met 
    530                 535                 540                 


His Gly Glu Asp His Ile Asn Gly Gly Ser Gly Ala Ile Trp Lys Tyr 
545                 550                 555                 560 


Gly Gln Ser Glu Val Arg Pro Cys Ala Ser Leu Glu Leu Arg Leu Asn 
                565                 570                 575     


<210>  43
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic Citrine protein sequence

<400>  43

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Phe Gly Tyr Gly Leu Met Cys Phe Ala Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Ala Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 


<210>  44
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic spacer protein sequence

<400>  44

Ala Ala Ala Ala Ser Gly Ser Gly Ser Gly Ser 
1               5                   10      


<210>  45
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic TEVsite protein sequence

<400>  45

Glu Asn Leu Tyr Phe Gln Phe 
1               5           


<210>  46
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic Ndeg protein sequence

<400>  46

His Lys Ser Gly Ala Trp Lys Leu Pro Val Ser Leu Val Lys 
1               5                   10                  


<210>  47
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic spacer protein sequence

<400>  47

Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Ser Ile Ala 
1               5                   10              


<210>  48
<211>  292
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic WUS protein sequence

<400>  48

Met Glu Pro Pro Gln His Gln His His His His Gln Ala Asp Gln Glu 
1               5                   10                  15      


Ser Gly Asn Asn Asn Asn Asn Lys Ser Gly Ser Gly Gly Tyr Thr Cys 
            20                  25                  30          


Arg Gln Thr Ser Thr Arg Trp Thr Pro Thr Thr Glu Gln Ile Lys Ile 
        35                  40                  45              


Leu Lys Glu Leu Tyr Tyr Asn Asn Ala Ile Arg Ser Pro Thr Ala Asp 
    50                  55                  60                  


Gln Ile Gln Lys Ile Thr Ala Arg Leu Arg Gln Phe Gly Lys Ile Glu 
65                  70                  75                  80  


Gly Lys Asn Val Phe Tyr Trp Phe Gln Asn His Lys Ala Arg Glu Arg 
                85                  90                  95      


Gln Lys Lys Arg Phe Asn Gly Thr Asn Met Thr Thr Pro Ser Ser Ser 
            100                 105                 110         


Pro Asn Ser Val Met Met Ala Ala Asn Asp His Tyr His Pro Leu Leu 
        115                 120                 125             


His His His His Gly Val Pro Met Gln Arg Pro Ala Asn Ser Val Asn 
    130                 135                 140                 


Val Lys Leu Asn Gln Asp His His Leu Tyr His His Asn Lys Pro Tyr 
145                 150                 155                 160 


Pro Ser Phe Asn Asn Gly Asn Leu Asn His Ala Ser Ser Gly Thr Glu 
                165                 170                 175     


Cys Gly Val Val Asn Ala Ser Asn Gly Tyr Met Ser Ser His Val Tyr 
            180                 185                 190         


Gly Ser Met Glu Gln Asp Cys Ser Met Asn Tyr Asn Asn Val Gly Gly 
        195                 200                 205             


Gly Trp Ala Asn Met Asp His His Tyr Ser Ser Ala Pro Tyr Asn Phe 
    210                 215                 220                 


Phe Asp Arg Ala Lys Pro Leu Phe Gly Leu Glu Gly His Gln Glu Glu 
225                 230                 235                 240 


Glu Glu Cys Gly Gly Asp Ala Tyr Leu Glu His Arg Arg Thr Leu Pro 
                245                 250                 255     


Leu Phe Pro Met His Gly Glu Asp His Ile Asn Gly Gly Ser Gly Ala 
            260                 265                 270         


Ile Trp Lys Tyr Gly Gln Ser Glu Val Arg Pro Cys Ala Ser Leu Glu 
        275                 280                 285             


Leu Arg Leu Asn 
    290         


<210>  49
<211>  2481
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic CenH3 degron DNA sequence for Arabidopsis:Citrine 
       spacer TEVsite Ndeg spacer CenH3 stop

<400>  49
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac       60

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac      120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc      180

ctcgtgacca ccttcggcta cggcctgatg tgcttcgccc gctaccccga ccacatgaag      240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc      300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg      360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac      420

aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac      480

ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc      540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac      600

tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc      660

ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggca      720

gcggccgctt cagggagtgg ttccggaagc gaaaacttgt acttccaatt ccacaagtct      780

ggtgcttgga agttgccagt ttctttggtt aaggggagtg gttccggcag cggctctgga      840

tcgtcaattg ctatggcgag aaccaagcat cgcgttacca ggtcacaacc tcggaatcaa      900

actggtatct taaatctgct ttctctttca atttttactt ctgattttac ccagaatttt      960

aggtttttta tttcgatttt gttaacccta gatttcgaat ctgaaatttg tagatgccgc     1020

cggtgcttca tcttctcagg cggcaggtcc aactacggta cggcatcttt ttccgtctta     1080

gggtttccaa tgtttcttcc ttttatcgtt atgatcaaat ttgtttatct atcgaaattg     1140

aagaccccga caaggagagg cggtgaaggt ggagataata ctcaacaaag tgagtttttt     1200

atatttgaag tctttttttt ccctcttttc atctcttttg tttgtgaagt tattcttttg     1260

taacatctgc agcaaatcct acaacttcac cagctactgg tacaagggta agatttttgt     1320

gaccattgct tatgaactgc ttcaactttg atttcgttat taagctgaca aaattctcgt     1380

tttggtttgt caagagaggg gctaagagat ccagacaggc tatgccacga ggtttgtttt     1440

aaaaaaaaaa ccaatctctt gtgatatccc tgagaataca ggacacttag tgtgtttaaa     1500

actaatcttc ggtgttgtcc ttgtaggctc acagaagaag tcttatcgat acaggccagg     1560

aaccgttgct ctaaaagaga ttcgccattt ccagaagcag acaaaccttc ttattccggc     1620

tgccagtttc ataagagaag ttagttactc tttttcttac cagccataat aagtttcaca     1680

gcttaacaat attcatatat actaacagag gcacaagcct tttggtgttt aatgtggcta     1740

gttttaggat ttgcacaccc cacacatatc tgagcatcaa tgcagtgtac atagtgagtg     1800

atatagcaat ttaactaaaa ttcagagtaa tcgtgaggcc aaccctcctt gtttaaggag     1860

tgtgtaatct agtttgtctt tgaggttatg agctcataga ttcagaacca tatgattcct     1920

gtagctacaa aactcaacat gaatcgtcag tgatgtggaa atgctgattt gtgttacaaa     1980

caaactattt tacattgttt ttccaggtga gaagtataac ccatatgttg gcccctcccc     2040

aaatcaatcg ttggacagct gaagctcttg ttgctcttca agaggtacca atccttcaac     2100

tttttcttta tacgaatgta tgaatataga tatagagata gtcacacatt tcaactaatg     2160

tcattcccct tgatgaccaa tcaacctaat cacacaaatt ctttgtggta ggcggcagaa     2220

gattacttgg ttggtttgtt ctcagattca atgctctgtg ctatccatgc aagacgtgtt     2280

actctaagta agtactctaa aagaagacat ttttcagtct caacttagga atcacaagca     2340

tacattttat atccctttga atcattagtt acttgaatat catatataaa aatgcttatc     2400

tatatctgtt ttttgttcat atcagtgaga aaagactttg aacttgcacg ccggcttgga     2460

ggaaaaggca gaccatggtg a                                               2481


<210>  50
<211>  717
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic Citrine DNA sequence (CenH3 depletion)

<400>  50
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac       60

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac      120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc      180

ctcgtgacca ccttcggcta cggcctgatg tgcttcgccc gctaccccga ccacatgaag      240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc      300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg      360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac      420

aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac      480

ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc      540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac      600

tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc      660

ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaag         717


<210>  51
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic spacer DNA sequence (CenH3 depletion)

<400>  51
gcagcggccg cttcagggag tggttccgga agc                                    33


<210>  52
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic TEVsite DNA sequence (CenH3 depletion)

<400>  52
gaaaacttgt acttccaatt c                                                 21


<210>  53
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic Ndeg DNA sequence (CenH3 depletion)

<400>  53
cacaagtctg gtgcttggaa gttgccagtt tctttggtta ag                          42


<210>  54
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic spacer DNA sequence (CenH3 depletion)

<400>  54
gggagtggtt ccggcagcgg ctctggatcg tcaattgct                              39


<210>  55
<211>  1626
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic CenH3 DNA sequence (CenH3 depletion)

<400>  55
atggcgagaa ccaagcatcg cgttaccagg tcacaacctc ggaatcaaac tggtatctta       60

aatctgcttt ctctttcaat ttttacttct gattttaccc agaattttag gttttttatt      120

tcgattttgt taaccctaga tttcgaatct gaaatttgta gatgccgccg gtgcttcatc      180

ttctcaggcg gcaggtccaa ctacggtacg gcatcttttt ccgtcttagg gtttccaatg      240

tttcttcctt ttatcgttat gatcaaattt gtttatctat cgaaattgaa gaccccgaca      300

aggagaggcg gtgaaggtgg agataatact caacaaagtg agttttttat atttgaagtc      360

ttttttttcc ctcttttcat ctcttttgtt tgtgaagtta ttcttttgta acatctgcag      420

caaatcctac aacttcacca gctactggta caagggtaag atttttgtga ccattgctta      480

tgaactgctt caactttgat ttcgttatta agctgacaaa attctcgttt tggtttgtca      540

agagaggggc taagagatcc agacaggcta tgccacgagg tttgttttaa aaaaaaaacc      600

aatctcttgt gatatccctg agaatacagg acacttagtg tgtttaaaac taatcttcgg      660

tgttgtcctt gtaggctcac agaagaagtc ttatcgatac aggccaggaa ccgttgctct      720

aaaagagatt cgccatttcc agaagcagac aaaccttctt attccggctg ccagtttcat      780

aagagaagtt agttactctt tttcttacca gccataataa gtttcacagc ttaacaatat      840

tcatatatac taacagaggc acaagccttt tggtgtttaa tgtggctagt tttaggattt      900

gcacacccca cacatatctg agcatcaatg cagtgtacat agtgagtgat atagcaattt      960

aactaaaatt cagagtaatc gtgaggccaa ccctccttgt ttaaggagtg tgtaatctag     1020

tttgtctttg aggttatgag ctcatagatt cagaaccata tgattcctgt agctacaaaa     1080

ctcaacatga atcgtcagtg atgtggaaat gctgatttgt gttacaaaca aactatttta     1140

cattgttttt ccaggtgaga agtataaccc atatgttggc ccctccccaa atcaatcgtt     1200

ggacagctga agctcttgtt gctcttcaag aggtaccaat ccttcaactt tttctttata     1260

cgaatgtatg aatatagata tagagatagt cacacatttc aactaatgtc attccccttg     1320

atgaccaatc aacctaatca cacaaattct ttgtggtagg cggcagaaga ttacttggtt     1380

ggtttgttct cagattcaat gctctgtgct atccatgcaa gacgtgttac tctaagtaag     1440

tactctaaaa gaagacattt ttcagtctca acttaggaat cacaagcata cattttatat     1500

ccctttgaat cattagttac ttgaatatca tatataaaaa tgcttatcta tatctgtttt     1560

ttgttcatat cagtgagaaa agactttgaa cttgcacgcc ggcttggagg aaaaggcaga     1620

ccatgg                                                                1626


