                         SEQUENCE LISTING

<110>  Regeneron Pharmaceuticals, Inc.
 
<120>  Assays For Screening Activity Of Modulators Of Members Of The 
       Hydroxysteroid (17-beta) Dehydrogenase (HSD17B) Family

<130>  189238.00102 (3024)

<150>  62/484,141
<151>  2017-04-11

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  300
<212>  PRT
<213>  artificial sequence

<220>
<223>  artificial sequence

<400>  1

Met Asn Ile Ile Leu Glu Ile Leu Leu Leu Leu Ile Thr Ile Ile Tyr 
1               5                   10                  15      

Ser Tyr Leu Glu Ser Leu Val Lys Phe Phe Ile Pro Gln Arg Arg Lys 
            20                  25                  30          

Ser Val Ala Gly Glu Ile Val Leu Ile Thr Gly Ala Gly His Gly Ile 
        35                  40                  45              

Gly Arg Gln Thr Thr Tyr Glu Phe Ala Lys Arg Gln Ser Ile Leu Val 
    50                  55                  60                  

Leu Trp Asp Ile Asn Lys Arg Gly Val Glu Glu Thr Ala Ala Glu Cys 
65                  70                  75                  80  

Arg Lys Leu Gly Val Thr Ala His Ala Tyr Val Val Asp Cys Ser Asn 
                85                  90                  95      

Arg Glu Glu Ile Tyr Arg Ser Leu Asn Gln Val Lys Lys Glu Val Gly 
            100                 105                 110         

Asp Val Thr Ile Val Val Asn Asn Ala Gly Thr Val Tyr Pro Ala Asp 
        115                 120                 125             

Leu Leu Ser Thr Lys Asp Glu Glu Ile Thr Lys Thr Phe Glu Val Asn 
    130                 135                 140                 

Ile Leu Gly His Phe Trp Ile Thr Lys Ala Leu Leu Pro Ser Met Met 
145                 150                 155                 160 

Glu Arg Asn His Gly His Ile Val Thr Val Ala Ser Val Cys Gly His 
                165                 170                 175     

Glu Gly Ile Pro Tyr Leu Ile Pro Tyr Cys Ser Ser Lys Phe Ala Ala 
            180                 185                 190         

Val Gly Phe His Arg Gly Leu Thr Ser Glu Leu Gln Ala Leu Gly Lys 
        195                 200                 205             

Thr Gly Ile Lys Thr Ser Cys Leu Cys Pro Val Phe Val Asn Thr Gly 
    210                 215                 220                 

Phe Thr Lys Asn Pro Ser Thr Arg Leu Trp Pro Val Leu Glu Thr Asp 
225                 230                 235                 240 

Glu Val Val Arg Ser Leu Ile Asp Gly Ile Leu Thr Asn Lys Lys Met 
                245                 250                 255     

Ile Phe Val Pro Ser Tyr Ile Asn Ile Phe Leu Arg Leu Gln Lys Phe 
            260                 265                 270         

Leu Pro Glu Arg Ala Ser Ala Ile Leu Asn Arg Met Gln Asn Ile Gln 
        275                 280                 285             

Phe Glu Ala Val Val Gly His Lys Ile Lys Met Lys 
    290                 295                 300 


<210>  2
<211>  264
<212>  PRT
<213>  artificial sequence

<220>
<223>  artificial sequence

<400>  2

Met Asn Ile Ile Leu Glu Ile Leu Leu Leu Leu Ile Thr Ile Ile Tyr 
1               5                   10                  15      

Ser Tyr Leu Glu Ser Leu Val Lys Phe Phe Ile Pro Gln Arg Arg Lys 
            20                  25                  30          

Ser Val Ala Gly Glu Ile Val Leu Ile Thr Gly Ala Gly His Gly Ile 
        35                  40                  45              

Gly Arg Gln Thr Thr Tyr Glu Phe Ala Lys Arg Gln Ser Ile Leu Val 
    50                  55                  60                  

Leu Trp Asp Ile Asn Lys Val Lys Lys Glu Val Gly Asp Val Thr Ile 
65                  70                  75                  80  

Val Val Asn Asn Ala Gly Thr Val Tyr Pro Ala Asp Leu Leu Ser Thr 
                85                  90                  95      

Lys Asp Glu Glu Ile Thr Lys Thr Phe Glu Val Asn Ile Leu Gly His 
            100                 105                 110         

Phe Trp Ile Thr Lys Ala Leu Leu Pro Ser Met Met Glu Arg Asn His 
        115                 120                 125             

Gly His Ile Val Thr Val Ala Ser Val Cys Gly His Glu Gly Ile Pro 
    130                 135                 140                 

Tyr Leu Ile Pro Tyr Cys Ser Ser Lys Phe Ala Ala Val Gly Phe His 
145                 150                 155                 160 

Arg Gly Leu Thr Ser Glu Leu Gln Ala Leu Gly Lys Thr Gly Ile Lys 
                165                 170                 175     

Thr Ser Cys Leu Cys Pro Val Phe Val Asn Thr Gly Phe Thr Lys Asn 
            180                 185                 190         

Pro Ser Thr Arg Leu Trp Pro Val Leu Glu Thr Asp Glu Val Val Arg 
        195                 200                 205             

Ser Leu Ile Asp Gly Ile Leu Thr Asn Lys Lys Met Ile Phe Val Pro 
    210                 215                 220                 

Ser Tyr Ile Asn Ile Phe Leu Arg Leu Gln Lys Phe Leu Pro Glu Arg 
225                 230                 235                 240 

Ala Ser Ala Ile Leu Asn Arg Met Gln Asn Ile Gln Phe Glu Ala Val 
                245                 250                 255     

Val Gly His Lys Ile Lys Met Lys 
            260                 


<210>  3
<211>  261
<212>  PRT
<213>  artificial sequence

<220>
<223>  artificial sequence

<400>  3

Met Asn Ile Ile Leu Glu Ile Leu Leu Leu Leu Ile Thr Ile Ile Tyr 
1               5                   10                  15      

Ser Tyr Leu Glu Ser Leu Val Lys Phe Phe Ile Pro Gln Arg Arg Lys 
            20                  25                  30          

Ser Val Ala Gly Glu Ile Val Leu Ile Thr Gly Ala Gly His Gly Ile 
        35                  40                  45              

Gly Arg Gln Thr Thr Tyr Glu Phe Ala Lys Arg Gln Ser Ile Leu Val 
    50                  55                  60                  

Leu Trp Asp Ile Asn Lys Arg Gly Val Glu Glu Thr Ala Ala Glu Cys 
65                  70                  75                  80  

Arg Lys Leu Gly Val Thr Ala His Ala Tyr Val Val Asp Cys Ser Asn 
                85                  90                  95      

Arg Glu Glu Ile Tyr Arg Ser Leu Asn Gln Val Lys Lys Glu Val Gly 
            100                 105                 110         

Asp Val Thr Ile Val Val Asn Asn Ala Gly Thr Val Tyr Pro Ala Asp 
        115                 120                 125             

Leu Leu Ser Thr Lys Asp Glu Glu Ile Thr Lys Thr Phe Glu Val Asn 
    130                 135                 140                 

Ile Leu Gly His Phe Trp Ile Thr Lys Ala Leu Leu Pro Ser Met Met 
145                 150                 155                 160 

Glu Arg Asn His Gly His Ile Val Thr Val Ala Ser Val Cys Gly His 
                165                 170                 175     

Glu Gly Ile Pro Tyr Leu Ile Pro Tyr Cys Ser Ser Lys Phe Ala Ala 
            180                 185                 190         

Val Gly Phe His Arg Gly Leu Thr Ser Glu Leu Gln Ala Leu Gly Lys 
        195                 200                 205             

Thr Gly Ile Lys Thr Ser Cys Leu Cys Pro Val Phe Val Asn Thr Gly 
    210                 215                 220                 

Phe Thr Lys Asn Pro Ser Thr Arg Phe Leu Pro Glu Arg Ala Ser Ala 
225                 230                 235                 240 

Ile Leu Asn Arg Met Gln Asn Ile Gln Phe Glu Ala Val Val Gly His 
                245                 250                 255     

Lys Ile Lys Met Lys 
            260     


<210>  4
<211>  274
<212>  PRT
<213>  artificial sequence

<220>
<223>  artificial sequence

<400>  4

Met Asn Ile Ile Leu Glu Ile Leu Leu Leu Leu Ile Thr Ile Ile Tyr 
1               5                   10                  15      

Ser Tyr Leu Glu Ser Leu Val Lys Phe Phe Ile Pro Gln Arg Arg Lys 
            20                  25                  30          

Ser Val Ala Gly Glu Ile Val Leu Ile Thr Gly Ala Gly His Gly Ile 
        35                  40                  45              

Gly Arg Gln Thr Thr Tyr Glu Phe Ala Lys Arg Gln Ser Ile Leu Val 
    50                  55                  60                  

Leu Trp Asp Ile Asn Lys Arg Gly Val Glu Glu Thr Ala Ala Glu Cys 
65                  70                  75                  80  

Arg Lys Leu Gly Val Thr Ala His Ala Tyr Val Val Asp Cys Ser Asn 
                85                  90                  95      

Arg Glu Glu Ile Tyr Arg Ser Leu Asn Gln Val Lys Lys Glu Val Gly 
            100                 105                 110         

Asp Val Thr Ile Val Val Asn Asn Ala Gly Thr Val Tyr Pro Ala Asp 
        115                 120                 125             

Leu Leu Ser Thr Lys Asp Glu Glu Ile Thr Lys Thr Phe Glu Val Asn 
    130                 135                 140                 

Ile Leu Gly His Phe Trp Ile Thr Lys Ala Leu Leu Pro Ser Met Met 
145                 150                 155                 160 

Glu Arg Asn His Gly His Ile Val Thr Val Ala Ser Val Cys Gly His 
                165                 170                 175     

Glu Gly Ile Pro Tyr Leu Ile Pro Tyr Cys Ser Ser Lys Phe Ala Ala 
            180                 185                 190         

Val Gly Phe His Arg Gly Leu Thr Ser Glu Leu Gln Ala Leu Gly Lys 
        195                 200                 205             

Thr Gly Ile Lys Thr Ser Cys Leu Cys Pro Val Phe Val Asn Thr Gly 
    210                 215                 220                 

Phe Thr Lys Asn Pro Ser Thr Arg Leu Trp Pro Val Leu Glu Thr Asp 
225                 230                 235                 240 

Glu Val Val Arg Ser Leu Ile Asp Gly Ile Leu Thr Asn Lys Lys Met 
                245                 250                 255     

Ile Phe Val Pro Ser Tyr Ile Asn Ile Phe Leu Arg Leu Gln Lys Val 
            260                 265                 270         

Ser Ser 
        


<210>  5
<211>  324
<212>  PRT
<213>  artificial sequence

<220>
<223>  artificial sequence

<400>  5

Met Asn Ile Ile Leu Glu Ile Leu Leu Leu Leu Ile Thr Ile Ile Tyr 
1               5                   10                  15      

Ser Tyr Leu Glu Ser Leu Val Lys Phe Phe Ile Pro Gln Arg Arg Lys 
            20                  25                  30          

Ser Val Ala Gly Glu Ile Val Leu Ile Thr Gly Ala Gly His Gly Ile 
        35                  40                  45              

Gly Arg Gln Thr Thr Tyr Glu Phe Ala Lys Arg Gln Ser Ile Leu Val 
    50                  55                  60                  

Leu Trp Asp Ile Asn Lys Arg Gly Val Glu Glu Thr Ala Ala Glu Cys 
65                  70                  75                  80  

Arg Lys Leu Gly Val Thr Ala His Ala Tyr Val Val Asp Cys Ser Asn 
                85                  90                  95      

Arg Glu Glu Ile Tyr Arg Ser Leu Asn Gln Val Lys Lys Glu Val Gly 
            100                 105                 110         

Asp Val Thr Ile Val Val Asn Asn Ala Gly Thr Val Tyr Pro Ala Asp 
        115                 120                 125             

Leu Leu Ser Thr Lys Asp Glu Glu Ile Thr Lys Thr Phe Glu Val Asn 
    130                 135                 140                 

Ile Leu Gly His Phe Trp Asn Gly Lys Asp Ile Arg Ser Asn Tyr Leu 
145                 150                 155                 160 

Asp Val Tyr Arg Ile Glu Asp Thr Phe Gly Arg Asp Ser Glu Ile Thr 
                165                 170                 175     

Lys Ala Leu Leu Pro Ser Met Met Glu Arg Asn His Gly His Ile Val 
            180                 185                 190         

Thr Val Ala Ser Val Cys Gly His Glu Gly Ile Pro Tyr Leu Ile Pro 
        195                 200                 205             

Tyr Cys Ser Ser Lys Phe Ala Ala Val Gly Phe His Arg Gly Leu Thr 
    210                 215                 220                 

Ser Glu Leu Gln Ala Leu Gly Lys Thr Gly Ile Lys Thr Ser Cys Leu 
225                 230                 235                 240 

Cys Pro Val Phe Val Asn Thr Gly Phe Thr Lys Asn Pro Ser Thr Arg 
                245                 250                 255     

Leu Trp Pro Val Leu Glu Thr Asp Glu Val Val Arg Ser Leu Ile Asp 
            260                 265                 270         

Gly Ile Leu Thr Asn Lys Lys Met Ile Phe Val Pro Ser Tyr Ile Asn 
        275                 280                 285             

Ile Phe Leu Arg Leu Gln Lys Phe Leu Pro Glu Arg Ala Ser Ala Ile 
    290                 295                 300                 

Leu Asn Arg Met Gln Asn Ile Gln Phe Glu Ala Val Val Gly His Lys 
305                 310                 315                 320 

Ile Lys Met Lys 
                

<210>  6
<211>  284
<212>  PRT
<213>  artificial sequence

<220>
<223>  artificial sequence

<400>  6

Met Asn Ile Ile Leu Glu Ile Leu Leu Leu Leu Ile Thr Ile Ile Tyr 
1               5                   10                  15      

Ser Tyr Leu Glu Ser Leu Val Lys Phe Phe Ile Pro Gln Arg Arg Lys 
            20                  25                  30          

Ser Val Ala Gly Glu Ile Val Leu Ile Thr Gly Ala Gly His Gly Ile 
        35                  40                  45              

Gly Arg Gln Thr Thr Tyr Glu Phe Ala Lys Arg Gln Ser Ile Leu Val 
    50                  55                  60                  

Leu Trp Asp Ile Asn Lys Arg Gly Val Glu Glu Thr Ala Ala Glu Cys 
65                  70                  75                  80  

Arg Lys Leu Gly Val Thr Ala His Ala Tyr Val Val Asp Cys Ser Asn 
                85                  90                  95      

Arg Glu Glu Ile Tyr Arg Ser Leu Asn Gln Val Lys Lys Glu Val Gly 
            100                 105                 110         

Asp Val Thr Ile Val Val Asn Asn Ala Gly Thr Val Tyr Pro Ala Asp 
        115                 120                 125             

Leu Leu Ser Thr Lys Asp Glu Glu Ile Thr Lys Thr Phe Glu Val Asn 
    130                 135                 140                 

Ile Leu Gly His Phe Trp Ile Thr Lys Ala Leu Leu Pro Ser Met Met 
145                 150                 155                 160 

Glu Arg Asn His Gly His Ile Val Thr Val Ala Ser Val Cys Gly His 
                165                 170                 175     

Glu Gly Ile Pro Tyr Leu Ile Pro Tyr Cys Ser Ser Lys Phe Ala Ala 
            180                 185                 190         

Val Gly Phe His Arg Gly Leu Thr Ser Glu Leu Gln Ala Leu Gly Lys 
        195                 200                 205             

Thr Gly Ile Lys Thr Ser Cys Leu Cys Pro Val Phe Val Asn Thr Gly 
    210                 215                 220                 

Phe Thr Lys Asn Pro Ser Thr Arg Leu Trp Pro Val Leu Glu Thr Asp 
225                 230                 235                 240 

Glu Val Val Arg Ser Leu Ile Asp Gly Ile Leu Thr Asn Lys Lys Met 
                245                 250                 255     

Ile Phe Val Pro Ser Tyr Ile Asn Ile Phe Leu Arg Leu Gln Lys Leu 
            260                 265                 270         

Ser Thr Ala Gln Asn Thr Gln Ile Leu Lys His Gln 
        275                 280                 


<210>  7
<211>  271
<212>  PRT
<213>  artificial sequence

<220>
<223>  artificial sequence

<400>  7

Met Asn Ile Ile Leu Glu Ile Leu Leu Leu Leu Ile Thr Ile Ile Tyr 
1               5                   10                  15      

Ser Tyr Leu Glu Ser Leu Val Lys Phe Phe Ile Pro Gln Arg Arg Lys 
            20                  25                  30          

Ser Val Ala Gly Glu Ile Val Leu Ile Thr Gly Ala Gly His Gly Ile 
        35                  40                  45              

Gly Arg Gln Thr Thr Tyr Glu Phe Ala Lys Arg Gln Ser Ile Leu Val 
    50                  55                  60                  

Leu Trp Asp Ile Asn Lys Arg Gly Val Glu Glu Thr Ala Ala Glu Cys 
65                  70                  75                  80  

Arg Lys Leu Gly Val Thr Ala His Ala Tyr Val Val Asp Cys Ser Asn 
                85                  90                  95      

Arg Glu Glu Ile Tyr Arg Ser Leu Asn Gln Val Lys Lys Glu Val Gly 
            100                 105                 110         

Asp Val Thr Ile Val Val Asn Asn Ala Gly Thr Val Tyr Pro Ala Asp 
        115                 120                 125             

Leu Leu Ser Thr Lys Asp Glu Glu Ile Thr Lys Thr Phe Glu Val Asn 
    130                 135                 140                 

Ile Leu Gly His Phe Trp Ile Thr Lys Ala Leu Leu Pro Ser Met Met 
145                 150                 155                 160 

Glu Arg Asn His Gly His Ile Val Thr Val Ala Ser Val Cys Gly His 
                165                 170                 175     

Glu Gly Ile Pro Tyr Leu Ile Pro Tyr Cys Ser Ser Lys Phe Ala Ala 
            180                 185                 190         

Val Gly Phe His Arg Gly Leu Thr Ser Glu Leu Gln Ala Leu Gly Lys 
        195                 200                 205             

Thr Gly Ile Lys Thr Ser Cys Leu Cys Pro Val Phe Val Asn Thr Gly 
    210                 215                 220                 

Phe Thr Lys Asn Pro Ser Thr Arg Leu Trp Pro Val Leu Glu Thr Asp 
225                 230                 235                 240 

Glu Val Val Arg Ser Leu Ile Asp Gly Ile Leu Thr Asn Lys Lys Met 
                245                 250                 255     

Ile Phe Val Pro Ser Tyr Ile Asn Ile Phe Leu Arg Leu Gln Lys 
            260                 265                 270     


<210>  8
<211>  238
<212>  PRT
<213>  artificial sequence

<220>
<223>  artificial sequence

<400>  8

Met Asn Ile Ile Leu Glu Ile Leu Leu Leu Leu Ile Thr Ile Ile Tyr 
1               5                   10                  15      

Ser Tyr Leu Glu Ser Leu Val Lys Phe Phe Ile Pro Gln Arg Arg Lys 
            20                  25                  30          

Ser Val Ala Gly Glu Ile Val Leu Ile Thr Gly Ala Gly His Gly Ile 
        35                  40                  45              

Gly Arg Gln Thr Thr Tyr Glu Phe Ala Lys Arg Gln Ser Ile Leu Val 
    50                  55                  60                  

Leu Trp Asp Ile Asn Lys Val Lys Lys Glu Val Gly Asp Val Thr Ile 
65                  70                  75                  80  

Val Val Asn Asn Ala Gly Thr Val Tyr Pro Ala Asp Leu Leu Ser Thr 
                85                  90                  95      

Lys Asp Glu Glu Ile Thr Lys Thr Phe Glu Val Asn Ile Leu Gly His 
            100                 105                 110         

Phe Trp Ile Thr Lys Ala Leu Leu Pro Ser Met Met Glu Arg Asn His 
        115                 120                 125             

Gly His Ile Val Thr Val Ala Ser Val Cys Gly His Glu Gly Ile Pro 
    130                 135                 140                 

Tyr Leu Ile Pro Tyr Cys Ser Ser Lys Phe Ala Ala Val Gly Phe His 
145                 150                 155                 160 

Arg Gly Leu Thr Ser Glu Leu Gln Ala Leu Gly Lys Thr Gly Ile Lys 
                165                 170                 175     

Thr Ser Cys Leu Cys Pro Val Phe Val Asn Thr Gly Phe Thr Lys Asn 
            180                 185                 190         

Pro Ser Thr Arg Leu Trp Pro Val Leu Glu Thr Asp Glu Val Val Arg 
        195                 200                 205             

Ser Leu Ile Asp Gly Ile Leu Thr Asn Lys Lys Met Ile Phe Val Pro 
    210                 215                 220                 

Ser Tyr Ile Asn Ile Phe Leu Arg Leu Gln Lys Val Ser Ser 
225                 230                 235             


<210>  9
<211>  298
<212>  PRT
<213>  artificial sequence

<220>
<223>  artificial sequence

<400>  9

Met Asn Ile Ile Leu Glu Ile Leu Leu Leu Leu Ile Thr Ile Ile Tyr 
1               5                   10                  15      

Ser Tyr Leu Glu Ser Leu Val Lys Phe Phe Ile Pro Gln Arg Arg Lys 
            20                  25                  30          

Ser Val Ala Gly Glu Ile Val Leu Ile Thr Gly Ala Gly His Gly Ile 
        35                  40                  45              

Gly Arg Gln Thr Thr Tyr Glu Phe Ala Lys Arg Gln Ser Ile Leu Val 
    50                  55                  60                  

Leu Trp Asp Ile Asn Lys Arg Gly Val Glu Glu Thr Ala Ala Glu Cys 
65                  70                  75                  80  

Arg Lys Leu Gly Val Thr Ala His Ala Tyr Val Val Asp Cys Ser Asn 
                85                  90                  95      

Arg Glu Glu Ile Tyr Arg Ser Leu Asn Gln Val Lys Lys Glu Val Gly 
            100                 105                 110         

Asp Val Thr Ile Val Val Asn Asn Ala Gly Thr Val Tyr Pro Ala Asp 
        115                 120                 125             

Leu Leu Ser Thr Lys Asp Glu Glu Ile Thr Lys Thr Phe Glu Val Asn 
    130                 135                 140                 

Ile Leu Gly His Phe Trp Asn Gly Lys Asp Ile Arg Ser Asn Tyr Leu 
145                 150                 155                 160 

Asp Val Tyr Arg Ile Glu Asp Thr Phe Gly Arg Asp Ser Glu Ile Thr 
                165                 170                 175     

Lys Ala Leu Leu Pro Ser Met Met Glu Arg Asn His Gly His Ile Val 
            180                 185                 190         

Thr Val Ala Ser Val Cys Gly His Glu Gly Ile Pro Tyr Leu Ile Pro 
        195                 200                 205             

Tyr Cys Ser Ser Lys Phe Ala Ala Val Gly Phe His Arg Gly Leu Thr 
    210                 215                 220                 

Ser Glu Leu Gln Ala Leu Gly Lys Thr Gly Ile Lys Thr Ser Cys Leu 
225                 230                 235                 240 

Cys Pro Val Phe Val Asn Thr Gly Phe Thr Lys Asn Pro Ser Thr Arg 
                245                 250                 255     

Leu Trp Pro Val Leu Glu Thr Asp Glu Val Val Arg Ser Leu Ile Asp 
            260                 265                 270         

Gly Ile Leu Thr Asn Lys Lys Met Ile Phe Val Pro Ser Tyr Ile Asn 
        275                 280                 285             

Ile Phe Leu Arg Leu Gln Lys Val Ser Ser 
    290                 295             


