SEQUENCE LISTING

<110> Cellectis; Albert-Ludwig-Universitt Freiburg 

<120> NEW SEQUENCE SPECIFIC REAGENTS TARGETING CCR5 IN PRIMARY HEMATOPOIETIC CELLS

<130> P81701556PCT00

<150> PA201770267
<151> 2017-04-13

<160> 44

<170> PatentIn version 3.5

<210>  1

<211>  1056
<212>  DNA
<213>  homo sapiens

<220>
<223>  CCR5 coding sequence

<400>  1
atggattatc aagtgtcaag tccaatctat gacatcaatt attatacatc ggagccctgc     60
caaaaaatca atgtgaagca aatcgcagcc cgcctcctgc ctccgctcta ctcactggtg    120
ttcatctttg gttttgtggg caacatgctg gtcatcctca tcctgataaa ctgcaaaagg    180
ctgaagagca tgactgacat ctacctgctc aacctggcca tctctgacct gtttttcctt    240
cttactgtcc ccttctgggc tcactatgct gccgcccagt gggactttgg aaatacaatg    300
tgtcaactct tgacagggct ctattttata ggcttcttct ctggaatctt cttcatcatc    360
ctcctgacaa tcgataggta cctggctgtc gtccatgctg tgtttgcttt aaaagccagg    420
acggtcacct ttggggtggt gacaagtgtg atcacttggg tggtggctgt gtttgcgtct    480
ctcccaggaa tcatctttac cagatctcaa aaagaaggtc ttcattacac ctgcagctct    540
cattttccat acagtcagta tcaattctgg aagaatttcc agacattaaa gatagtcatc    600
ttggggctgg tcctgccgct gcttgtcatg gtcatctgct actcgggaat cctaaaaact    660
ctgcttcggt gtcgaaatga gaagaagagg cacagggctg tgaggcttat cttcaccatc    720
atgattgttt attttctctt ctgggctccc tacaacattg tccttctcct gaacaccttc    780
caggaattct ttggcctgaa taattgcagt agctctaaca ggttggacca agctatgcag    840
gtgacagaga ctcttgggat gacgcactgc tgcatcaacc ccatcatcta tgcctttgtc    900
ggggagaagt tcagaaacta cctcttagtc ttcttccaaa agcacattgc caaacgcttc    960
tgcaaatgct gttctatttt ccagcaagag gctcccgagc gagcaagctc agtttacacc   1020
cgatccactg gggagcagga aatatctgtg ggcttg                             1056


<210>  2

<211>  99
<212>  DNA
<213>  artificial sequence

<220>
<223>  polynucleotide sequence encoding 30 first CCR5 amino acids

<400>  2
atggattatc aagtgtcaag tccaatctat gacatcaatt attatacatc ggagccctgc     60
caaaaaatca atgtgaagca aatcgcagcc cgcctcctg                            99


<210>  3

<211>  60
<212>  DNA
<213>  artificial sequence

<220>
<223>  polynucleotide sequence encoding 20 first CCR5 amino acids

<400>  3
atggattatc aagtgtcaag tccaatctat gacatcaatt attatacatc ggagccctgc     60


<210>  4

<211>  48
<212>  DNA
<213>  artificial sequence

<220>
<223>  polynucleotide sequence encoding 2-18 CCR5 amino acids

<400>  4
tatcaagtgt caagtccaat ctatgacatc aattattata catcggag                  48


<210>  5

<211>  16
<212>  DNA
<213>  artificial sequence

<220>
<223>  TALEN T3L target sequence

<400>  5
tatcaagtgt caagtc                                                     16


<210>  6

<211>  16
<212>  DNA
<213>  artificial sequence

<220>
<223>  TALEN T3R target sequence

<400>  6
attattatac atcgga                                                     16


<210>  7

<211>  15
<212>  DNA
<213>  artificial sequence

<220>
<223>  TALEN T3 spacer sequence

<400>  7
caatctatga catca                                                      15


<210>  8

<211>  925
<212>  PRT
<213>  artificial sequence

<220>
<223>  TALEN T3L polypeptide sequence

<400>  8
Met Gly Asp Pro Lys Lys Lys Arg Lys Val Ile Asp Ile Ala Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Lys Tyr Gln Asp Met Ile Ala Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Ala Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Val Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Leu Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Val Glu Ala Val His Ala Trp Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Ala Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Ala Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro 
        275                 280                 285             


Glu Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Ala Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu 
305                 310                 315                 320 


Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His 
            340                 345                 350         


Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
            500                 505                 510         


Ala Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln 
        515                 520                 525             


Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr 
    530                 535                 540                 


Val Gln Ala Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro 
545                 550                 555                 560 


Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu 
                565                 570                 575     


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu 
            580                 585                 590         


Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln 
        595                 600                 605             


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His 
    610                 615                 620                 


Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser His Asp Gly Gly 
625                 630                 635                 640 


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
                645                 650                 655     


Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly 
            660                 665                 670         


Gly Gly Arg Pro Ala Leu Glu Ser Ile Val Ala Gln Leu Ser Arg Pro 
        675                 680                 685             


Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His Leu Val Ala Leu Ala 
    690                 695                 700                 


Cys Leu Gly Gly Arg Pro Ala Leu Asp Ala Val Lys Lys Gly Leu Gly 
705                 710                 715                 720 


Asp Pro Ile Ser Arg Ser Gln Leu Val Lys Ser Glu Leu Glu Glu Lys 
                725                 730                 735     


Lys Ser Glu Leu Arg His Lys Leu Lys Tyr Val Pro His Glu Tyr Ile 
            740                 745                 750         


Glu Leu Ile Glu Ile Ala Arg Asn Ser Thr Gln Asp Arg Ile Leu Glu 
        755                 760                 765             


Met Lys Val Met Glu Phe Phe Met Lys Val Tyr Gly Tyr Arg Gly Lys 
    770                 775                 780                 


His Leu Gly Gly Ser Arg Lys Pro Asp Gly Ala Ile Tyr Thr Val Gly 
785                 790                 795                 800 


Ser Pro Ile Asp Tyr Gly Val Ile Val Asp Thr Lys Ala Tyr Ser Gly 
                805                 810                 815     


Gly Tyr Asn Leu Pro Ile Gly Gln Ala Asp Glu Met Gln Arg Tyr Val 
            820                 825                 830         


Glu Glu Asn Gln Thr Arg Asn Lys His Ile Asn Pro Asn Glu Trp Trp 
        835                 840                 845             


Lys Val Tyr Pro Ser Ser Val Thr Glu Phe Lys Phe Leu Phe Val Ser 
    850                 855                 860                 


Gly His Phe Lys Gly Asn Tyr Lys Ala Gln Leu Thr Arg Leu Asn His 
865                 870                 875                 880 


Ile Thr Asn Cys Asn Gly Ala Val Leu Ser Val Glu Glu Leu Leu Ile 
                885                 890                 895     


Gly Gly Glu Met Ile Lys Ala Gly Thr Leu Thr Leu Glu Glu Val Arg 
            900                 905                 910         


Arg Lys Phe Asn Asn Gly Glu Ile Asn Phe Ala Ala Asp 
        915                 920                 925 


<210>  9

<211>  925
<212>  PRT
<213>  artificial sequence

<220>
<223>  TALEN T3R polypeptide sequence

<400>  9
Met Gly Asp Pro Lys Lys Lys Arg Lys Val Ile Asp Ile Ala Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Lys Tyr Gln Asp Met Ile Ala Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Ala Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Val Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Leu Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Val Glu Ala Val His Ala Trp Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Ala Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro 
        275                 280                 285             


Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu 
305                 310                 315                 320 


Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His 
            340                 345                 350         


Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Ile 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Ala Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Ala Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
            500                 505                 510         


Ala Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln 
        515                 520                 525             


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
    530                 535                 540                 


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro 
545                 550                 555                 560 


Glu Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu 
                565                 570                 575     


Glu Thr Val Gln Ala Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu 
            580                 585                 590         


Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln 
        595                 600                 605             


Ala Leu Glu Thr Val Gln Ala Leu Leu Pro Val Leu Cys Gln Ala His 
    610                 615                 620                 


Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly 
625                 630                 635                 640 


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
                645                 650                 655     


Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly 
            660                 665                 670         


Gly Gly Arg Pro Ala Leu Glu Ser Ile Val Ala Gln Leu Ser Arg Pro 
        675                 680                 685             


Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His Leu Val Ala Leu Ala 
    690                 695                 700                 


Cys Leu Gly Gly Arg Pro Ala Leu Asp Ala Val Lys Lys Gly Leu Gly 
705                 710                 715                 720 


Asp Pro Ile Ser Arg Ser Gln Leu Val Lys Ser Glu Leu Glu Glu Lys 
                725                 730                 735     


Lys Ser Glu Leu Arg His Lys Leu Lys Tyr Val Pro His Glu Tyr Ile 
            740                 745                 750         


Glu Leu Ile Glu Ile Ala Arg Asn Ser Thr Gln Asp Arg Ile Leu Glu 
        755                 760                 765             


Met Lys Val Met Glu Phe Phe Met Lys Val Tyr Gly Tyr Arg Gly Lys 
    770                 775                 780                 


His Leu Gly Gly Ser Arg Lys Pro Asp Gly Ala Ile Tyr Thr Val Gly 
785                 790                 795                 800 


Ser Pro Ile Asp Tyr Gly Val Ile Val Asp Thr Lys Ala Tyr Ser Gly 
                805                 810                 815     


Gly Tyr Asn Leu Pro Ile Gly Gln Ala Asp Glu Met Gln Arg Tyr Val 
            820                 825                 830         


Glu Glu Asn Gln Thr Arg Asn Lys His Ile Asn Pro Asn Glu Trp Trp 
        835                 840                 845             


Lys Val Tyr Pro Ser Ser Val Thr Glu Phe Lys Phe Leu Phe Val Ser 
    850                 855                 860                 


Gly His Phe Lys Gly Asn Tyr Lys Ala Gln Leu Thr Arg Leu Asn His 
865                 870                 875                 880 


Ile Thr Asn Cys Asn Gly Ala Val Leu Ser Val Glu Glu Leu Leu Ile 
                885                 890                 895     


Gly Gly Glu Met Ile Lys Ala Gly Thr Leu Thr Leu Glu Glu Val Arg 
            900                 905                 910         


Arg Lys Phe Asn Asn Gly Glu Ile Asn Phe Ala Ala Asp 
        915                 920                 925 

<210>  10

<211>  7445
<212>  DNA
<213>  artificial sequence

<220>
<223>  TALEN T3L plasmid sequence

<400>  10
aaacccacga cacctgaaat ggaagaaaaa aactttgaac cactgtctga ggcttgagaa     60
tgaaccaaga tccaaactca aaaagggcaa attccaagga gaattacatc aagtgccaag    120
ctggcctaac ttcagtctcc acccactcag tgtggggaaa ctccatcgca taaaacccct    180
ccccccaacc taaagacgac gtactccaaa agctcgagaa ctaatcgagg tgcctggacg    240
gcgcccggta ctccgtggag tcacatgaag cgacggctga ggacggaaag gcccttttcc    300
tttgtgtggg tgactcaccc gcccgctctc ccgagcgccg cgtcctccat tttgagctcc    360
ctgcagcagg gccgggaagc ggccatcttt ccgctcacgc aactggtgcc gaccgggcca    420
gccttgccgc ccagggcggg gcgatacacg gcggcgcgag gccaggcacc agagcaggcc    480
ggccagcttg agactacccc cgtccgattc tcggtggccg cgctcgcagg ccccgcctcg    540
ccgaacatgt gcgctgggac gcacgggccc cgtcgccgcc cgcggcccca aaaaccgaaa    600
taccagtgtg cagatcgatc ttggcccgca tttacaagac tatcttgcca gaaaaaaagc    660
gtcgcagcag gtcatcaaaa attttaaatg gctagagact tatcgaaagc agcgagacag    720
gcgcgaaggt gccaccagat tcgcacgcgg cggccccagc gcccaagcca ggcctcaact    780
caagcacgag gcgaaggggc tccttaagcg caaggcctcg aactctccca cccacttcca    840
acccgaagct cgggatcaag aatcacgtac tgcagccagg ggcgtggaag taattcaagg    900
cacgcaaggg ccataacccg taaagaggcc aggcccgcgg gaaccacaca cggcacttac    960
ctgtgttctg gcggcaaacc cgttgcgaaa aagaacgttc acggcgacta ctgcacttat   1020
atacggttct cccccaccct cgggaaaaag gcggagccag tacacgacat cactttccca   1080
gtttaccccg cgccaccttc tctaggcacc ggttcaattg ccgacccctc cccccaactt   1140
ctcggggact gtgggcgatg tgcgctctgc ccactgacgg gcaccggagc ctcacgcatg   1200
ctcttctcca cctcagtgat gacgagagcg ggcgggtgag ggggcgggaa cgcagcgatc   1260
tctgggttct acgttagtgg gagtttaacg acggtccctg ggattcccca aggcaggggc   1320
gagtcctttt gtatgaatta ctctcagctc cggtcggggc gggttggggg gggtggtgac   1380
ggggaggccg cctggaaggg acgtgcagaa tcttccctct accattgctg gcttagctcc   1440
aaaggttgta ttgagattag ggtgtacctt cgcctctcaa tcagcctccc gtcctcagcc   1500
ttgccatctc gctagtccgg gacaaatccc tagagcgtct tcctctgcgg gtctcagccc   1560
agcccggggt tggctcctcc tccgccccgg cttccgcgcc cctcccgtgt ggcaaggagt   1620
accaggcccg gggaccccga ggggcttggg gcgaagggtc gggactgggg gcctccttaa   1680
cggctcacgg acttgcgaga ggttcggctc gatggccgtg aaagcgacga atccgctcct   1740
gtgctggcct cttggctcct tccattcaaa gccagctgct tttatggaag cccgtaacac   1800
gtcatctccc cctggtactc cagatgtcca ggctttcagt ttagaataga ctcagtccta   1860
cagttagctt tggcgcgatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc   1920
tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag   1980
cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg   2040
cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tagtcaggtg   2100
gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa   2160
atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga   2220
agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc   2280
ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg   2340
gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc   2400
gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat   2460
tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg   2520
acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag   2580
aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa   2640
cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc   2700
gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca   2760
cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc   2820
tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc   2880
tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg   2940
ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta   3000
tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag   3060
gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga   3120
ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc   3180
tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa   3240
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa   3300
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc   3360
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt   3420
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc   3480
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac   3540
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca   3600
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg   3660
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag   3720
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt   3780
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat   3840
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc   3900
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt   3960
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag   4020
cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca   4080
gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga   4140
gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt   4200
gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca   4260
agctctaggt cttaattaag aagatatccc atagagccca ccgcatcccc agcatgcctg   4320
ctattgtctt cccaatcctc ccccttgctg tcctgcccca ccccaccccc cagaatagaa   4380
tgacacctac tcagacaatg cgatgcaatt tcctcatttt attaggaaag gacagtggga   4440
gtggcacctt ccagggtcaa ggaaggcacg ggggaggggc aaacaacaga tggctggcaa   4500
ctagaaggca cagtcgaagc ttcagctgct gcaggctcga ggagctcgtc tagaggatcg   4560
ctcgagttat cagtcggccg cgaagttgat ctcgccgttg ttgaacttcc tcctcacctc   4620
ctccagggtc agggtgccgg ccttgatcat ctcgccgccg atcaggagct cctccacgga   4680
cagcacggcg ccgttgcagt tggtgatgtg gttcagcctg gtcagctggg ccttgtagtt   4740
gcccttgaag tggccggaca cgaacaggaa cttgaactcg gtcacgctgg aggggtacac   4800
cttccaccac tcgttggggt tgatgtgctt gttcctggtc tggttctcct ccacgtacct   4860
ctgcatttcg tcggcctggc cgatgggcag gttgtagccg ccggagtagg ccttggtgtc   4920
cacgatcacg ccgtagtcga tgggggagcc cacggtgtag atggcgccgt cgggcttcct   4980
ggagccgccc aggtgcttgc ccctgtagcc gtacaccttc atgaagaact ccatcacctt   5040
catctccagg atacggtcct gggtgctgtt ccgggcgatc tcgatcagct cgatgtactc   5100
gtggggcacg tacttcagct tgtgcctcaa ctcggatttc ttctcctcca gctcggactt   5160
caccagctgg gaacggctga taggatcccc caatcccttt ttcactgcat ccagcgcagg   5220
acgcccgccg aggcaggcca aggcgacgag gtggtcgttg gtcaacgcgg ccaacgccgg   5280
atcagggcga gataactggg caacaatgct ctcgagggcg ggcctaccgc ctccattaga   5340
agctatagcg accacctgct gcggtgtgag cccatgagcc tgacacaaaa caggcaacaa   5400
tcgctgcacc gtttccaggg cctgttttcc gccgtcatgg ctcgcgatag caacaacctg   5460
ttcgggtgtc agcccgtgtg cttgacagag aacaggcaac agcctctgga ctgtctccaa   5520
tgcctgtttc cctccgccat tgcttgcaat tgcgacgact tgctgtgggg tcagaccgtg   5580
tgcctgacac agcacgggca gcaggcgctg gactgtttcg agggcttgct tgccaccgtt   5640
attggatgcg atggccacaa cttgttgtgg ggtaagcccg tgcgcctggc acaacactgg   5700
cagcaacgcc tgaactgttt ccagcgcttg cttaccgcca atattgctgg cgatcgctac   5760
cacttgttcg ggagtcaggc catgagcctg gcacagaacg ggcaggaggg cctgaacggt   5820
ttcgagggcc tgcttgcctc caatgttaga ggcaatggcg accacctgtt caggggtaag   5880
gccgtgagcc tggcagagca caggcagcag cctctgcact gtttccaatg cttgcttccc   5940
tccgtcatga gaagcgatag cgacaacttg ctcgggggtc aggccatgtg cttggcagag   6000
aactgggagc aacctttgca cagtctcgag tgcctgcttt ccgcctccgt tgctagcaat   6060
agccacaacc tgttgtgggg tcaggccgtg cgcctgacac aggacgggaa ggagtctttg   6120
caccgtttcg agtgcctgtt taccgccgtt gtttgatgcg attgccacga cctgctgggg   6180
tgtgagtcca tgagcttgac agaggaccgg aaggagtctc tggacggttt ccagtgcttg   6240
tttacctcct ccgtttgaag cgattgcaac cacttgttgc ggggtaagtc catgcgcttg   6300
gcagagcacc ggcagcagtc tctgtacggt ttccagagct tgcttgccgc cattattact   6360
ggcaatagcc accacctgtt ggggagtgag gccgtgagct tggcagagta caggcaacag   6420
agcctgcacg gtttcgagcg cctgcttccc gccaatgttt gaggcaatag cgactacttg   6480
ttcgggtgta aggccgtggg cctgacacaa gactggcagg agagcctgta cggtctcgag   6540
agcctgcttc ccaccgatat tggacgcgat ggctacgacc tgctcaggcg tcagtccgtg   6600
ggcttgacac aaaacgggca gcagccgctg cacagtctcc agggcttgct ttcctccgtc   6660
gtgtgaagct atggccacga cctgttcagg agtcagccca tgagcttggc acaatactgg   6720
gagaagtctc tgcactgtct ccagggcctg cttccctcct ccattgctag cgatggccac   6780
cacttgctgt ggagtcagcc cgtgggcctg gcaaagcaca ggaagcaagg cctgaacagt   6840
ctcgagcgct tgtttccctc cgatattaga agcaatagca acgacctgtt ctggggtcaa   6900
gttgagcggg gcacccgtca gtgcattgcg ccatgcatgc actgcctcca ctgcggtcac   6960
gccgccacgt tttgcaatct tgagaagttg gcctgtgtcc aactgtaacg gtggacctct   7020
caactctccc gccaccgtga gcaaggcctc cagagcgcgt gcgccggacc actgtttgcc   7080
gacgccaacg atcgcttcgt gtgtcgcctc tggcaacgct gcgatcatgt cctgatactt   7140
gacagcgacg gtccctaacg ctgccgggtg ttggcttaac gcaacgatgt gcgcgtgtgt   7200
aaacccgtgg ccgaccagtg cctcgtggtg ctgcgccact gtcgaacgaa ccttcggttt   7260
gatcttctcc tgttgctgct ggctgtagcc gagcgtgcgt agatcggcga tatcgatagc   7320
gtaatctgga acatcgtatg ggtaatcgat gaccttacgt ttctttttag gatcgcccat   7380
ggtggcggct tggcgcgtga cagcgctagt ggcgcgccga actagttatg gtaccaagtt   7440
taaac                                                               7445


<210>  11

<211>  7463
<212>  DNA
<213>  artificial sequence

<220>
<223>  TALEN T3R plasmid sequence

<400>  11
aaacccacga cacctgaaat ggaagaaaaa aactttgaac cactgtctga ggcttgagaa     60
tgaaccaaga tccaaactca aaaagggcaa attccaagga gaattacatc aagtgccaag    120
ctggcctaac ttcagtctcc acccactcag tgtggggaaa ctccatcgca taaaacccct    180
ccccccaacc taaagacgac gtactccaaa agctcgagaa ctaatcgagg tgcctggacg    240
gcgcccggta ctccgtggag tcacatgaag cgacggctga ggacggaaag gcccttttcc    300
tttgtgtggg tgactcaccc gcccgctctc ccgagcgccg cgtcctccat tttgagctcc    360
ctgcagcagg gccgggaagc ggccatcttt ccgctcacgc aactggtgcc gaccgggcca    420
gccttgccgc ccagggcggg gcgatacacg gcggcgcgag gccaggcacc agagcaggcc    480
ggccagcttg agactacccc cgtccgattc tcggtggccg cgctcgcagg ccccgcctcg    540
ccgaacatgt gcgctgggac gcacgggccc cgtcgccgcc cgcggcccca aaaaccgaaa    600
taccagtgtg cagatcgatc ttggcccgca tttacaagac tatcttgcca gaaaaaaagc    660
gtcgcagcag gtcatcaaaa attttaaatg gctagagact tatcgaaagc agcgagacag    720
gcgcgaaggt gccaccagat tcgcacgcgg cggccccagc gcccaagcca ggcctcaact    780
caagcacgag gcgaaggggc tccttaagcg caaggcctcg aactctccca cccacttcca    840
acccgaagct cgggatcaag aatcacgtac tgcagccagg ggcgtggaag taattcaagg    900
cacgcaaggg ccataacccg taaagaggcc aggcccgcgg gaaccacaca cggcacttac    960
ctgtgttctg gcggcaaacc cgttgcgaaa aagaacgttc acggcgacta ctgcacttat   1020
atacggttct cccccaccct cgggaaaaag gcggagccag tacacgacat cactttccca   1080
gtttaccccg cgccaccttc tctaggcacc ggttcaattg ccgacccctc cccccaactt   1140
ctcggggact gtgggcgatg tgcgctctgc ccactgacgg gcaccggagc ctcacgcatg   1200
ctcttctcca cctcagtgat gacgagagcg ggcgggtgag ggggcgggaa cgcagcgatc   1260
tctgggttct acgttagtgg gagtttaacg acggtccctg ggattcccca aggcaggggc   1320
gagtcctttt gtatgaatta ctctcagctc cggtcggggc gggttggggg gggtggtgac   1380
ggggaggccg cctggaaggg acgtgcagaa tcttccctct accattgctg gcttagctcc   1440
aaaggttgta ttgagattag ggtgtacctt cgcctctcaa tcagcctccc gtcctcagcc   1500
ttgccatctc gctagtccgg gacaaatccc tagagcgtct tcctctgcgg gtctcagccc   1560
agcccggggt tggctcctcc tccgccccgg cttccgcgcc cctcccgtgt ggcaaggagt   1620
accaggcccg gggaccccga ggggcttggg gcgaagggtc gggactgggg gcctccttaa   1680
cggctcacgg acttgcgaga ggttcggctc gatggccgtg aaagcgacga atccgctcct   1740
gtgctggcct cttggctcct tccattcaaa gccagctgct tttatggaag cccgtaacac   1800
gtcatctccc cctggtactc cagatgtcca ggctttcagt ttagaataga ctcagtccta   1860
cagttagctt tggcgcgatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc   1920
tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag   1980
cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg   2040
cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tagtcaggtg   2100
gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa   2160
atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga   2220
agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc   2280
ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg   2340
gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc   2400
gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat   2460
tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg   2520
acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag   2580
aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa   2640
cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc   2700
gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca   2760
cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc   2820
tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc   2880
tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg   2940
ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta   3000
tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag   3060
gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga   3120
ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc   3180
tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa   3240
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa   3300
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc   3360
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt   3420
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc   3480
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac   3540
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca   3600
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg   3660
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag   3720
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt   3780
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat   3840
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc   3900
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt   3960
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag   4020
cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca   4080
gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga   4140
gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt   4200
gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca   4260
agctctaggt cttaattaag aagatatccc atagagccca ccgcatcccc agcatgcctg   4320
ctattgtctt cccaatcctc ccccttgctg tcctgcccca ccccaccccc cagaatagaa   4380
tgacacctac tcagacaatg cgatgcaatt tcctcatttt attaggaaag gacagtggga   4440
gtggcacctt ccagggtcaa ggaaggcacg ggggaggggc aaacaacaga tggctggcaa   4500
ctagaaggca cagtcgaagc ttcagctgct gcaggctcga ggagctcgtc tagaggatcg   4560
ctcgagttat cagtcggccg cgaagttgat ctcgccgttg ttgaacttcc tcctcacctc   4620
ctccagggtc agggtgccgg ccttgatcat ctcgccgccg atcaggagct cctccacgga   4680
cagcacggcg ccgttgcagt tggtgatgtg gttcagcctg gtcagctggg ccttgtagtt   4740
gcccttgaag tggccggaca cgaacaggaa cttgaactcg gtcacgctgg aggggtacac   4800
cttccaccac tcgttggggt tgatgtgctt gttcctggtc tggttctcct ccacgtacct   4860
ctgcatttcg tcggcctggc cgatgggcag gttgtagccg ccggagtagg ccttggtgtc   4920
cacgatcacg ccgtagtcga tgggggagcc cacggtgtag atggcgccgt cgggcttcct   4980
ggagccgccc aggtgcttgc ccctgtagcc gtacaccttc atgaagaact ccatcacctt   5040
catctccagg atacggtcct gggtgctgtt ccgggcgatc tcgatcagct cgatgtactc   5100
gtggggcacg tacttcagct tgtgcctcaa ctcggatttc ttctcctcca gctcggactt   5160
caccagctgg gaacggctga taggatcccc caatcccttt ttcactgcat ccagcgcagg   5220
acgcccgccg aggcaggcca aggcgacgag gtggtcgttg gtcaacgcgg ccaacgccgg   5280
atcagggcga gataactggg caacaatgct ctccaaagcg ggtcgccctc cgccgttact   5340
agcgattgcc acaacttgct gtggagtgag tccatgcgcc tggcagagca caggcagaag   5400
gcgctggacc gtttccaagg cttgcttccc accgccatta gaggctatgg cgaccacttg   5460
ctgtggggtc agaccgtgtg cttgacacaa gacaggcaac agagcttgaa cggtttcaag   5520
tgcctgcttt cccccaatat tggatgcgat tgcaaccact tgctcaggag tcaggccgtg   5580
tgcctgacaa agcacaggca gcaatgcctg aacggtttcc aatgcttgct tcccgccgat   5640
gttggaagca attgctacca cttgctcggg ggtgaggccg tgtgcttggc acagtaccgg   5700
cagcagccgt tggacggtct cgagagcctg tttcccgcct ccattactag cgatagccac   5760
tacttgctgg ggtgtgagcc cgtgggcttg gcaaagcaca ggaaggaggg cttgtactgt   5820
ttcgagcgcc tgcttcccgc ctatgttgga ggcgattgct accacctgct cgggggtcag   5880
tccgtgggct tgacacaaca cagggaggag agcttggaca gtttcgaggg cctgtttgcc   5940
gcctatgttt gaagcaatgg ccacgacttg ctctggtgtc aaaccgtgag cctgacagag   6000
cactgggagg agtctctgta cggtttcgag ggcttgcttg ccgcctccgt tggacgcaat   6060
ggcaacgacc tgctggggag tcaagccgtg cgcctgacaa agtacgggca acagcgcctg   6120
gaccgtttcg agagcctgct ttccgccgat attactggca atagcaacca cttgttcagg   6180
tgtaagaccg tgcgcctggc acagtactgg caacagccgt tgcacggttt ccagtgcctg   6240
cttgcccccg ccatttgagg ctatcgctac gacttgctga ggtgtcagtc cgtgcgcttg   6300
acagaggacg ggcagcagcc tttgcacagt ttccagtgct tgtttgccac cattattgga   6360
agcgatggcc acgacctgct gtggagtaag cccgtgagcc tggcaaagca cgggcagcag   6420
gcgctgcact gtctccaggg cctgtttccc tcccccgttg ctagcgatgg caaccacctg   6480
ctgaggggtc aagccgtggg cctggcacag cacagggaga agcgcctgga cagtctccag   6540
cgcctgtttc ccacctatgt tgcttgcgat cgccaccacc tgctctggcg tcaagccatg   6600
tgcttggcaa aggacgggga gcaagcgctg aacggtttcg agagcttgct tccctccatt   6660
gttggatgca attgctacta cctgctgtgg tgtcagcccg tgggcctgac aaagaactgg   6720
cagcaatcgc tgcactgttt ccaaggcctg cttaccccca tcatgacttg cgattgcgac   6780
gacctgctct ggtgtaagac catgtgcctg gcaaaggaca ggcaggagtc tctgcactgt   6840
ctcgagtgct tgtttacctc catcatgaga agcaatagcc accacttgtt cgggggtcaa   6900
gttgagcggg gcacccgtca gtgcattgcg ccatgcatgc actgcctcca ctgcggtcac   6960
gccgccacgt tttgcaatct tgagaagttg gcctgtgtcc aactgtaacg gtggacctct   7020
caactctccc gccaccgtga gcaaggcctc cagagcgcgt gcgccggacc actgtttgcc   7080
gacgccaacg atcgcttcgt gtgtcgcctc tggcaacgct gcgatcatgt cctgatactt   7140
gacagcgacg gtccctaacg ctgccgggtg ttggcttaac gcaacgatgt gcgcgtgtgt   7200
aaacccgtgg ccgaccagtg cctcgtggtg ctgcgccact gtcgaacgaa ccttcggttt   7260
gatcttctcc tgttgctgct ggctgtagcc gagcgtgcgt agatcggcga tatcgatgct   7320
gtccatgtgc tgtctctcga acttggcagc ggcggtctcc ttatcgatga ccttacgttt   7380
ctttttagga tcgcccatgg tggcggcttg gcgcgtgaca gcgctagtgg cgcgccgaac   7440
tagttatggt accaagttta aac                                           7463


<210>  12

<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  CLS_CCR5gRNA1

<400>  12
tgacatcaat tattatacat cgg                                             23


<210>  13

<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  CLS_CCR5gRNA2

<400>  13
tttgcttcac attgattttt tgg                                             23


<210>  14

<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  CLS_CCR5gRNA3

<400>  14
cttcacattg attttttggc agg                                             23


<210>  15

<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  CLS_CCR5gRNA4

<400>  15
ttcacattga ttttttggca ggg                                             23


<210>  16

<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  CLS_CCR5gRNA5

<400>  16
taataattga tgtcatagat tgg                                             23


<210>  17

<211>  19
<212>  DNA
<213>  artificial sequence

<220>
<223>  TALEN T1L target sequence

<400>  17
tgtgggcaac atgctggtc                                                  19


<210>  18

<211>  8344
<212>  DNA
<213>  artificial sequence

<220>
<223>  TALEN T1L plasmid sequence

<400>  18
gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta     60
aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata    120
ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc    180
ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga    240
agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct    300
tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg    360
tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta    420
ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat    480
gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt    540
acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga    600
tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga    660
gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga    720
actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc    780
aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc    840
cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg    900
tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat    960
cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata   1020
tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct   1080
ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga   1140
ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg   1200
cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc   1260
aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct   1320
agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc   1380
tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt   1440
ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg   1500
cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca   1560
ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag   1620
ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag   1680
tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg   1740
gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg   1800
gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac   1860
cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt   1920
gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat   1980
tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc   2040
aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc   2100
tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca   2160
tgattacgcc aagctctagc tagaggtcga cggtatacag acatgataag atacattgat   2220
gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt   2280
gatgctattg ctttatttgt aaccattata agctgcaata aacaagttgg ggtgggcgaa   2340
gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc ggaaaacgat   2400
tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgta gcacgtgtca   2460
gtcctgctcc tcggccacga agtgcttagc cctcccacac ataaccagag ggcagcaatt   2520
cacgaatccc aactgccgtc ggctgtccat cactgtcctt cactatcgct ttgatcccag   2580
gatgcagatc gagaagcacc tgtcgacacc gtccgcaggg gctcaagatg cccctgttct   2640
catttccgat cgcgacgata caagtcaggt tgccagctgc cgcagcagca gcagtgccca   2700
gcaccacgag ttctgcacaa ggtcccccag taaaatgata tacattgaca ccagtgaaga   2760
tgcggccgtc gctagagaga gctgcgctgg cgacgctgta gtcttcagag atggggatgc   2820
tgttgattgt agccgttgct ctttcaatga gggtggattc ttcttgagac aaaggcttgg   2880
ccatggttta gttcctcacc ttgtcgtatt atactatgcc gatatactat gccgatgatt   2940
aattgtcaac acgtgctgat cagatccgaa aatggatata caagctcccg ggagcttttt   3000
gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggcaga   3060
ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc   3120
ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga   3180
ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca   3240
cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgcct ggggagcctg   3300
gggactttcc acaccctaac tgacacacat tccacagaat taattcgcgt taaatttttg   3360
ttaaatcagc tcatttttta accaataggc cgaaatcccc aaaatccctt ataaatcaaa   3420
agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa   3480
gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg   3540
tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa   3600
ccctaaaggg atgccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa   3660
ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct   3720
gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc   3780
cctagagccc cagctggttc tttccgcctc agaagccata gagcccaccg catccccagc   3840
atgcctgcta ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc caccccccag   3900
aatagaatga cacctactca gacaatgcga tgcaatttcc tcattttatt aggaaaggac   3960
agtgggagtg gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg   4020
ctggcaacta gaaggcacag tcgaggctga tcagcgggtt taaactcaat ggtgatggtg   4080
atgatgaccg gtacgcgtag aatcgagacc gaggagaggg ttagggatag gcttaccttc   4140
gaagggccct taaaagttta tctcgccgtt attaaatttc cgtctcactt cctctaaggt   4200
taatgtgccg gctttaatca tttctccacc aattaaaagc tcttctacac taagaacagc   4260
tccattacaa ttagtgatat gatttaatcg tgtaagctga gctttgtagt ttcctttaaa   4320
gtgaccactc acaaataaaa acttaaattc cgttacagaa gatggataga ctttccacca   4380
ttcattaggg ttgatatgtt tgtttcgtgt ttgattttct tcgacatatc gttgcatttc   4440
atctgcttgg ccaattggca gattataacc tccgctataa gctttagtat ccacgatcac   4500
accgtaatca ataggagatc cgacagtata aattgctccg tccggtttcc ttgatccacc   4560
caaatgttta cctctatatc cataaacttt cataaaaaat tccattacct tcatttcaag   4620
aattctatcc tgagtggaat ttctggcaat ttcaattaat tcaatatatt catgaggcac   4680
atatttcaat ttatgacgaa gttcagattt cttctcctcc agttcacttt tgactagttg   4740
ggatccacta gtagtcaacg tgatcaaggc cggcgcgtgc ggcaatccct ttttcactgc   4800
atccagcgca ggacgtccgc cgaggcaggc caaggcgacg aggtggtcgt tggtcaacgc   4860
ggccaacgcc ggatcagggc gagataactg ggcaacaatg ctctccagcg ccggcctgcc   4920
gccgccattg ctggcgatgg ccaccacctg ctggggtgtc aggccatggg cctggcacag   4980
caccggcaac agcgcctgca ccgtctccag cgcctgcttg ccgccgccat tgctggcgat   5040
ggccaccacc tgctgcgggg tcaggccatg ggcctggcac agcaccggca acagccgctg   5100
caccgtctcc agcgcctgct tgcccccatt attgctggcg atggccacca cctgctccgg   5160
ggtcaggcca tgggcctggc acagcaccgg caacagccgc tgcaccgtct ccagagcctg   5220
cttgccacca atattgctgg cgatggccac cacctgctcc ggggtcaggc catgggcctg   5280
gcacagcacc ggcaacagcc gctgcaccgt ctcaagcgcc tgcttgccac cgtcgtggct   5340
ggcgatggcc accacctgct ccggggtcag gccatgggcc tggcacagca ccggcaacag   5400
ccgctggaca gtctccagcg cctgcttgcc accattattg ctggcgatgg ccaccacctg   5460
ctccggggtc aggccatggg cctggcacag caccggcaac aatcgctgca ccgtctccag   5520
cgcctgcttg ccaccgccat tgctggcgat ggccaccacc tgctgcgggg tcaggccatg   5580
ggcctggcac agcaccggaa gcagccgctg caccgtctcc agcgcctgct tgccaccgcc   5640
attgctggcg atcgctacca cctgctgggg tgtcaggcca tgggcctggc acagcaccgg   5700
caacagccgc tgcaccgtct ccagcgcctg cttgccaccg ccattgctgg cgatggctac   5760
cacctgttgc ggggtcaggc catgggcctg gcacagcacc ggcaacagcc gctgcaccgt   5820
ctccagcgcc tgcttgccac cgccattgct ggcgattgcc accacctgct ccggggtcag   5880
gccatgggcc tggcacagca ccggcaacag cgcctgcacc gtctccagcg cctgcttgcc   5940
accgtcgtgg cttgcgatgg ccaccacctg ctccggggtc aggccatggg cctggcacag   6000
caccggcaac agcgcctgca ccgtctccag cgcctgcttg ccgccgtcgt ggctggcgat   6060
ggccaccacc tgctgcgggg tcaggccatg ggcctggcac agcaccggca acagccgctg   6120
caccgtctcc agcgcctgct tgcccccatt attgctggcg atggccacca cctgctccgg   6180
ggtcaggcca tgggcctggc acagcaccgg caacagccgc tgcaccgtct ccagagcctg   6240
cttgccacca atattgctgg cgatggccac cacctgctcc ggggtcaggc catgggcctg   6300
gcacagcacc ggcaacagcc gctgcaccgt ctcaagcgcc tgcttgccac cgtcgtggct   6360
ggcgatggcc accacctgct ccggggtcag gccatgggcc tggcacagca ccggcaacag   6420
ccgctggaca gtctccagcg cctgcttgcc accgccattg ctggcgatgg ccaccacctg   6480
ctccggggtc aggccatggg cctggcacag caccggcaac aatcgctgca ccgtctccag   6540
cgcctgcttg ccaccgccat tgctggcgat ggccaccacc tgctgcgggg tcaggccatg   6600
ggcctggcac agcaccggaa gcagccgctg caccgtctcc agcgcctgct tgccgccatc   6660
gtggctggcg atggccacca cctgctgcgg cgtaaggttc aggggggcac ccgtcagtgc   6720
attgcgccat gcatgcactg cctccactgc ggtcacgccg ccacgttttg caatcttgag   6780
aagttggcct gtgtccaact gtaacggtgg acctctcaac tctcccgcca ccgtgagcaa   6840
ggcctccaga gcgcgtgcgc cggaccactg tttgccgacg ccaacgatcg cttcgtgtgt   6900
cgcctctggc aacgctgcga tcatgtcctg atacttgaca gcgacggtcc ctaacgctgc   6960
cgggtgttgg ctgagcgcaa cgatgtgcgc gtgtgtaaac ccatggccga ccagtgcctc   7020
gtggtgctgc gccactgtcg aacgaacctt cggtttgatc ttctcctgtt gctgctggct   7080
gtagccgagc gtgcgtagat ccacctgcgc ggccggcgaa gcgtcggagg gttgcgcagc   7140
acgtcgtcgc ggcgccagat ctaagctagc gtggatgccc actttccgct ttttcttggg   7200
tctagaggcg tagtcaggca cgtcgtaagg gtagcccatc ctagacacct gtggagagaa   7260
aggcaaagtg gatgttattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt   7320
tatgtattaa ttgtagccgc gttctaacga agccaagggg gtgggccata gactctatag   7380
gcggtactta cgtcactctt ggcacgggga atccgcgttc caatgcaccg ttcccggccg   7440
cggaggctgg atcggtcccg gtgtcttcta tggaggtcaa aacagcgtgg atggcgtctc   7500
caggcgatct gacggttcac taaacgagct ctgcttatat agacctccca ccgtacacgg   7560
ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa gtgccgttga   7620
ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt   7680
gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac catggtaata   7740
gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg   7800
gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata   7860
tgatacactt gatgtactgc caagtgggca gtttaccgta aatagtccac ccattgacgt   7920
caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg   7980
cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac   8040
tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata actagtcaat   8100
aatcaatgtc aacgcgtata tctggcccgt acatcgcgaa gcagcgcaaa acgcctaacc   8160
ctaagcagat tcttcatgca attgtcggtc aagccttgcc ttgttgtagc ttaaattttg   8220
ctcgcgcact actcagcgac ctccaacaca caagcaggga gcagatactg gcttaactat   8280
gcggcatcag agcagattgt actgagagtc gaccataggg gatcgggaga tctcccgatc   8340
cgtc                                                                8344


<210>  19

<211>  19
<212>  DNA
<213>  artificial sequence

<220>
<223>  TALEN T1R target sequence

<400>  19
tcttcagcct tttgcagtt                                                  19


<210>  20

<211>  7120
<212>  DNA
<213>  artificial sequence

<220>
<223>  TALEN T1R plasmid sequence

<400>  20
gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta     60
aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata    120
ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc    180
ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga    240
agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct    300
tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg    360
tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta    420
ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat    480
gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt    540
acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga    600
tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga    660
gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga    720
actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc    780
aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc    840
cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg    900
tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat    960
cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata   1020
tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct   1080
ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga   1140
ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg   1200
cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc   1260
aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct   1320
agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc   1380
tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt   1440
ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg   1500
cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca   1560
ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag   1620
ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag   1680
tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg   1740
gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg   1800
gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac   1860
cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt   1920
gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat   1980
tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc   2040
aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc   2100
tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca   2160
tgattacgcc aagctctagc tagaggtcga cggtatacag acatgataag atacattgat   2220
gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt   2280
gatgctattg ctttatttgt aaccattata agctgcaata aacaagttgg ggtgggcgaa   2340
gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc ggaaaacgat   2400
tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgta gcacgtgtca   2460
gtcctgctcc tcggccacga agtgcttagc cctcccacac ataaccagag ggcagcaatt   2520
cacgaatccc aactgccgtc ggctgtccat cactgtcctt cactatcgct ttgatcccag   2580
gatgcagatc gagaagcacc tgtcgacacc gtccgcaggg gctcaagatg cccctgttct   2640
catttccgat cgcgacgata caagtcaggt tgccagctgc cgcagcagca gcagtgccca   2700
gcaccacgag ttctgcacaa ggtcccccag taaaatgata tacattgaca ccagtgaaga   2760
tgcggccgtc gctagagaga gctgcgctgg cgacgctgta gtcttcagag atggggatgc   2820
tgttgattgt agccgttgct ctttcaatga gggtggattc ttcttgagac aaaggcttgg   2880
ccatggttta gttcctcacc ttgtcgtatt atactatgcc gatatactat gccgatgatt   2940
aattgtcaac acgtgctgat cagatccgaa aatggatata caagctcccg ggagcttttt   3000
gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggcaga   3060
ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc   3120
ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga   3180
ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca   3240
cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgcct ggggagcctg   3300
gggactttcc acaccctaac tgacacacat tccacagaat taattcgcgt taaatttttg   3360
ttaaatcagc tcatttttta accaataggc cgaaatcccc aaaatccctt ataaatcaaa   3420
agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa   3480
gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg   3540
tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa   3600
ccctaaaggg atgccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa   3660
ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct   3720
gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc   3780
cctagagccc cagctggttc tttccgcctc agaagccata gagcccaccg catccccagc   3840
atgcctgcta ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc caccccccag   3900
aatagaatga cacctactca gacaatgcga tgcaatttcc tcattttatt aggaaaggac   3960
agtgggagtg gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg   4020
ctggcaacta gaaggcacag tcgaggctga tcagcgggtt taaactcaat ggtgatggtg   4080
atgatgaccg gtacgcgtag aatcgagacc gaggagaggg ttagggatag gcttaccttc   4140
gaagggccct taaaagttta tctcgccgtt attaaatttc cgtctcactt cctctaaggt   4200
taatgtgccg gctttaatca tttctccacc aattaaaagc tcttctacac taagaacagc   4260
tccattacaa ttagtgatat gatttaatcg tgtaagctga gctttgtagt ttcctttaaa   4320
gtgaccactc acaaataaaa acttaaattc cgttacagaa gatggataga ctttccacca   4380
ttcattaggg ttgatatgtt tgtttcgtgt ttgattttct tcgacatatc gttgcatttc   4440
atctgcttgg ccaattggca gattataacc tccgctataa gctttagtat ccacgatcac   4500
accgtaatca ataggagatc cgacagtata aattgctccg tccggtttcc ttgatccacc   4560
caaatgttta cctctatatc cataaacttt cataaaaaat tccattacct tcatttcaag   4620
aattctatcc tgagtggaat ttctggcaat ttcaattaat tcaatatatt catgaggcac   4680
atatttcaat ttatgacgaa gttcagattt cttctcctcc agttcacttt tgactagttg   4740
ggatccacta gtagtcaacg tgatcaaggc cggcgcgtgc ggcaatccct ttttcactgc   4800
atccagcgca ggacgtccgc cgaggcaggc caaggcgacg aggtggtcgt tggtcaacgc   4860
ggccaacgcc ggatcagggc gagataactg ggcaacaatg ctctccagcg ccggcctgcc   4920
gccgccattg ctggcgatgg ccaccacctg ctggggtgtc aggccatggg cctggcacag   4980
caccggcaac agcgcctgca ccgtctccag cgcctgcttg ccgccgccat tgctggcgat   5040
ggccaccacc tgctgcgggg tcaggccatg ggcctggcac agcaccggca acagccgctg   5100
caccgtctcc agcgcctgct tgcccccatt attgctggcg atggccacca cctgctccgg   5160
ggtcaggcca tgggcctggc acagcaccgg caacagccgc tgcaccgtct ccagagcctg   5220
cttgccacca atattgctgg cgatggccac cacctgctcc ggggtcaggc catgggcctg   5280
gcacagcacc ggcaacagcc gctgcaccgt ctcaagcgcc tgcttgccac cgtcgtggct   5340
ggcgatggcc accacctgct ccggggtcag gccatgggcc tggcacagca ccggcaacag   5400
ccgctggaca gtctccagcg cctgcttgcc accattattg ctggcgatgg ccaccacctg   5460
ctccggggtc aggccatggg cctggcacag caccggcaac aatcgctgca ccgtctccag   5520
cgcctgcttg ccaccgccat tgctggcgat ggccaccacc tgctgcgggg tcaggccatg   5580
ggcctggcac agcaccggaa gcagccgctg caccgtctcc agcgcctgct tgccaccgcc   5640
attgctggcg atcgctacca cctgctgggg tgtcaggcca tgggcctggc acagcaccgg   5700
caacagccgc tgcaccgtct ccagcgcctg cttgccaccg ccattgctgg cgatggctac   5760
cacctgttgc ggggtcaggc catgggcctg gcacagcacc ggcaacagcc gctgcaccgt   5820
ctccagcgcc tgcttgccac cgccattgct ggcgattgcc accacctgct ccggggtcag   5880
gccatgggcc tggcacagca ccggcaacag cgcctgcacc gtctccagcg cctgcttgcc   5940
accgtcgtgg cttgcgatgg ccaccacctg ctccggggtc aggccatggg cctggcacag   6000
caccggcaac agcgcctgca ccgtctccag cgcctgcttg ccgccgtcgt ggctggcgat   6060
ggccaccacc tgctgcgggg tcaggccatg ggcctggcac agcaccggca acagccgctg   6120
caccgtctcc agcgcctgct tgcccccatt attgctggcg atggccacca cctgctccgg   6180
ggtcaggcca tgggcctggc acagcaccgg caacagccgc tgcaccgtct ccagagcctg   6240
cttgccacca atattgctgg cgatggccac cacctgctcc ggggtcaggc catgggcctg   6300
gcacagcacc ggcaacagcc gctgcaccgt ctcaagcgcc tgcttgccac cgtcgtggct   6360
ggcgatggcc accacctgct ccggggtcag gccatgggcc tggcacagca ccggcaacag   6420
ccgctggaca gtctccagcg cctgcttgcc accgccattg ctggcgatgg ccaccacctg   6480
ctccggggtc aggccatggg cctggcacag caccggcaac aatcgctgca ccgtctccag   6540
cgcctgcttg ccaccgccat tgctggcgat ggccaccacc tgctgcgggg tcaggccatg   6600
ggcctggcac agcaccggaa gcagccgctg caccgtctcc agcgcctgct tgccgccatc   6660
gtggctggcg atggccacca cctgctgcgg cgtaaggttc aggggggcac ccgtcagtgc   6720
attgcgccat gcatgcactg cctccactgc ggtcacgccg ccacgttttg caatcttgag   6780
aagttggcct gtgtccaact gtaacggtgg acctctcaac tctcccgcca ccgtgagcaa   6840
ggcctccaga gcgcgtgcgc cggaccactg tttgccgacg ccaacgatcg cttcgtgtgt   6900
cgcctctggc aacgctgcga tcatgtcctg atacttgaca gcgacggtcc ctaacgctgc   6960
cgggtgttgg ctgagcgcaa cgatgtgcgc gtgtgtaaac ccatggccga ccagtgcctc   7020
gtggtgctgc gccactgtcg aacgaacctt cggtttgatc ttctcctgtt gctgctggct   7080
gtagccgagc gtgcgtagat ccacctgcgc ggccggcgaa                         7120


<210>  21

<211>  19
<212>  DNA
<213>  artificial sequence

<220>
<223>  TALEN T2L target sequence

<400>  21
tttgtgggca acatgctgg                                                  19


<210>  22

<211>  5885
<212>  DNA
<213>  artificial sequence

<220>
<223>  TALEN T2L plasmid sequence

<400>  22
aagaacatgt gagcaaaagg ccagcaaaag cccaggaacc gtaaaaaggc cgcgttgctg     60
gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag    120
aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc    180
gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg    240
ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt    300
cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc    360
ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc    420
actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg    480
tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca    540
gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc    600
ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat    660
cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt    720
ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt    780
tttagcacgt gctattattg aagcatttat cagggttatt gtctcatgag cggatacata    840
tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg    900
ccacctgtat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga    960
aattgtaagc gttaataatt cagaagaact cgtcaagaag gcgatagaag gcgatgcgct   1020
gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa   1080
gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca   1140
gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc   1200
aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgctcgcc ttgagcctgg   1260
cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa   1320
gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg   1380
ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt   1440
tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca   1500
gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg   1560
tggccagcca cgatagccgc gctgcctcgt cttgcagttc attcagggca ccggacaggt   1620
cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag   1680
agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg   1740
gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt   1800
gatcagagct tgatcccctg cgccatcaga tccttggcgg cgagaaagcc atccagttta   1860
ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg   1920
ctgtccataa aaccgcccag tagaagccat agagcccacc gcatccccag catgcctgct   1980
attttcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca gaatagaatg   2040
acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga cagtgggagt   2100
ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg gctggcaact   2160
agaaggcaca gtcgaggctg atcagcgggt ttaaacgggc ccttaaaagt ttatctcgcc   2220
gttattaaat ttccgtctca cttcctctaa ggttaatgtg ccggctttaa tcatttctcc   2280
accaattaaa agctcttcta cactaagaac agctccatta caattagtga tatgatttaa   2340
tcgtgtaagc tgagctttgt agtttccttt aaagtgacca ctcacaaata aaaacttaaa   2400
ttccgttaca gaagatggat agactttcca ccattcatta gggttgatat gtttgtttcg   2460
tgtttgattt tcttcgacat atcgttgcat ttcatctgct tggccaattg gcagattata   2520
acctccgcta taagctttag tatccacgat cacaccgtaa tcaataggag atccgacagt   2580
ataaattgct ccgtccggtt tccttgatcc acccaaatgt ttacctctat atccataaac   2640
tttcataaaa aattccatta ccttcatttc aagaattcta tcctgagtgg aatttctggc   2700
aatttcaatt aattcaatat attcatgagg cacatatttc aatttatgac gaagttcaga   2760
tttcttctcc tccagttcac ttttgactag ttgggatcct gtcagggcgg ccagagctgg   2820
gtcgggtctg ctcagctggg ccacaataga ttccagggcg ggtctgcctc ccttgttgct   2880
ggcaatggcc acgacctgct ggggtgtcag gccatgggcc tggcacagca ccggcaacag   2940
cgcctgcacc gtctccagcg cctgcttgcc gccattattg ctggcgatgg ccaccacctg   3000
ctgcggggtc aggccatggg cctggcacag caccggcaac agccgctgca ccgtctccag   3060
cgcctgcttg cccccgccat tgctggcgat ggccaccacc tgctccgggg tcaggccatg   3120
ggcctggcac agcaccggca acagccgctg caccgtctcc agagcctgct tgccaccgtc   3180
gtggctggcg atggccacca cctgctgcgg ggtcaggcca tgggcctggc acagcaccgg   3240
caacagccgc tgcaccgtct caagcgcctg cttgccacca ttattgctgg cgatggccac   3300
cacctgctcc ggggtcaggc catgggcctg gcacagcacc ggcaacagcc gctggacagt   3360
ctccagcgcc tgcttgccac cgccattgct ggcgatggcc accacctgct ccggggtcag   3420
gccatgggcc tggcacagca ccggcaacaa tcgctgcacc gtctccagcg cctgcttgcc   3480
accaatattg ctggcgatgg ccaccacctg ctccggggtc aggccatggg cctggcacag   3540
caccggaagc agccgctgca ccgtctccag cgcctgcttg ccaccgtcgt ggctggcgat   3600
cgctaccacc tgctggggtg tcaggccatg ggcctggcac agcaccggca acagccgctg   3660
caccgtctcc agcgcctgct tgccaccaat attgctggcg atggctacca cctgttgcgg   3720
ggtcaggcca tgggcctggc acagcaccgg caacagccgc tgcaccgtct ccagcgcctg   3780
cttgccacca atattgctgg cgattgccac cacctgctcc ggggtcaggc catgggcctg   3840
gcacagcacc ggcaacagcg cctgcaccgt ctccagcgcc tgcttgccac cgtcgtggct   3900
tgcgatggcc accacctgct ccggggtcag gccatgggcc tggcacagca ccggcaacag   3960
cgcctgcacc gtctccagcg cctgcttgcc gccattattg ctggcgatgg ccaccacctg   4020
ctgcggggtc aggccatggg cctggcacag caccggcaac agccgctgca ccgtctccag   4080
cgcctgcttg cccccattat tgctggcgat ggccaccacc tgctccgggg tcaggccatg   4140
ggcctggcac agcaccggca acagccgctg caccgtctcc agagcctgct tgccaccatt   4200
attgctggcg atggccacca cctgctgcgg ggtcaggcca tgggcctggc acagcaccgg   4260
caacagccgc tgcaccgtct caagcgcctg cttgccaccg ccattgctgg cgatggccac   4320
cacctgctcc ggggtcaggc catgggcctg gcacagcacc ggcaacagcc gctggacagt   4380
ctccagcgcc tgcttgccac cattattgct ggcgatggcc accacctgct ccggggtcag   4440
gccatgggcc tggcacagca ccggcaacaa tcgctgcacc gtctccagcg cctgcttgcc   4500
accgccattg ctggcgatgg ccaccacctg ctgcggggtc aggccatggg cctggcacag   4560
caccggaagc agccgctgca ccgtctccag cgcctgcttg ccaccgccat tgctggcgat   4620
ggccaccacc tgctgcggcg taaggttcag gggagcgcct gtcagggcat tccgccaagc   4680
gtgcacggct tccacggctg tcacgcctcc ccgcttggcg atcttcagca gctggccggt   4740
gtccagctgc agtggagggc ctctcagctc gccggccact gtcagcaagg cctcgagtgc   4800
tctagcgccg gaccactgct tgcccacgcc cacgatagcc tcgtgtgtgg cctcgggcag   4860
ggcggcgatc atgtcctggt acttcacggc cacggttccc agagcggcag gatgctggct   4920
cagggccacg atgtgagcgt gggtaaagcc gtggcccacg agggcttcgt ggtgctgggc   4980
cacggtgctc cgcactttgg gcttgatctt ttcctgctgc tgctggctgt agcccagggt   5040
tctcaggtcc acctgggcgg caggagaggc atcggaaggc tgagcggccc ttcttctggg   5100
ggcgctagcg tggatgccca ctttccgctt tttcttgggt ctagaggcgt agtcaggcac   5160
gtcgtaaggg tagcccatga tatcactagc cagcttgggt ctccctatag tgagtcgtat   5220
taatttcgat aagccagtaa gcagtgggtt ctctagttag ccagagagct ctgcttatat   5280
agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac   5340
attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt   5400
ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa   5460
accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt   5520
cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa   5580
tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta   5640
aatagtccac ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg   5700
tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg   5760
taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt   5820
actattaata actagtcaat aatcaatgtc aacgcgtata tctggcccgt acatcgcgaa   5880
gagtc                                                               5885


<210>  23

<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  TALEN T2R target sequence

<400>  23
tcgagccttt tgcagtttat                                                 20


<210>  24

<211>  5885
<212>  DNA
<213>  artificial sequence

<220>
<223>  TALEN T2R plasmid sequence

<400>  24
aagaacatgt gagcaaaagg ccagcaaaag cccaggaacc gtaaaaaggc cgcgttgctg     60
gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag    120
aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc    180
gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg    240
ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt    300
cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc    360
ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc    420
actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg    480
tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca    540
gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc    600
ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat    660
cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt    720
ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt    780
tttagcacgt gctattattg aagcatttat cagggttatt gtctcatgag cggatacata    840
tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg    900
ccacctgtat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga    960
aattgtaagc gttaataatt cagaagaact cgtcaagaag gcgatagaag gcgatgcgct   1020
gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa   1080
gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca   1140
gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc   1200
aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgctcgcc ttgagcctgg   1260
cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa   1320
gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg   1380
ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt   1440
tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca   1500
gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg   1560
tggccagcca cgatagccgc gctgcctcgt cttgcagttc attcagggca ccggacaggt   1620
cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag   1680
agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg   1740
gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt   1800
gatcagagct tgatcccctg cgccatcaga tccttggcgg cgagaaagcc atccagttta   1860
ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg   1920
ctgtccataa aaccgcccag tagaagccat agagcccacc gcatccccag catgcctgct   1980
attttcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca gaatagaatg   2040
acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga cagtgggagt   2100
ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg gctggcaact   2160
agaaggcaca gtcgaggctg atcagcgggt ttaaacgggc ccttaaaagt ttatctcgcc   2220
gttattaaat ttccgtctca cttcctctaa ggttaatgtg ccggctttaa tcatttctcc   2280
accaattaaa agctcttcta cactaagaac agctccatta caattagtga tatgatttaa   2340
tcgtgtaagc tgagctttgt agtttccttt aaagtgacca ctcacaaata aaaacttaaa   2400
ttccgttaca gaagatggat agactttcca ccattcatta gggttgatat gtttgtttcg   2460
tgtttgattt tcttcgacat atcgttgcat ttcatctgct tggccaattg gcagattata   2520
acctccgcta taagctttag tatccacgat cacaccgtaa tcaataggag atccgacagt   2580
ataaattgct ccgtccggtt tccttgatcc acccaaatgt ttacctctat atccataaac   2640
tttcataaaa aattccatta ccttcatttc aagaattcta tcctgagtgg aatttctggc   2700
aatttcaatt aattcaatat attcatgagg cacatatttc aatttatgac gaagttcaga   2760
tttcttctcc tccagttcac ttttgactag ttgggatcct gtcagggcgg ccagagctgg   2820
gtcgggtctg ctcagctggg ccactataga ttccagggcg ggtctgcctc cgccgttgct   2880
ggcaatggcc acgacctgct ggggtgtcag gccatgggcc tggcacagca ccggcaacag   2940
cgcctgcacc gtctccagcg cctgcttgcc gccaatattg ctggcgatgg ccaccacctg   3000
ctgcggggtc aggccatggg cctggcacag caccggcaac agccgctgca ccgtctccag   3060
cgcctgcttg cccccgccat tgctggcgat ggccaccacc tgctccgggg tcaggccatg   3120
ggcctggcac agcaccggca acagccgctg caccgtctcc agagcctgct tgccaccgcc   3180
attgctggcg atggccacca cctgctccgg ggtcaggcca tgggcctggc acagcaccgg   3240
caacagccgc tgcaccgtct caagcgcctg cttgccaccg ccattgctgg cgatggccac   3300
cacctgctcc ggggtcaggc catgggcctg gcacagcacc ggcaacagcc gctggacagt   3360
ctccagcgcc tgcttgccac cattattgct ggcgatggcc accacctgct ccggggtcag   3420
gccatgggcc tggcacagca ccggcaacaa tcgctgcacc gtctccagcg cctgcttgcc   3480
accaatattg ctggcgatgg ccaccacctg ctccggggtc aggccatggg cctggcacag   3540
caccggaagc agccgctgca ccgtctccag cgcctgcttg ccaccgtcgt ggctggcgat   3600
cgctaccacc tgctggggtg tcaggccatg ggcctggcac agcaccggca acagccgctg   3660
caccgtctcc agcgcctgct tgccaccatt attgctggcg atggctacca cctgttgcgg   3720
ggtcaggcca tgggcctggc acagcaccgg caacagccgc tgcaccgtct ccagcgcctg   3780
cttgccaccg tcattgctgg cgattgccac cacctgctcc ggggtcaggc catgggcctg   3840
gcacagcaac ggaaacagcc cctgcaccgt ctcaagcgcc tgcttgccac cgccattgct   3900
ggcgatggcc accacctgct ccggggtcag gccatgggcc tggcacagca ccggcaacag   3960
cgcctgcacc gtctccagcg cctgcttgcc gccgccattg ctggcgatgg ccaccacctg   4020
ctgcggggtc aggccatggg cctggcacag caccggcaac agccgctgca ccgtctccag   4080
cgcctgcttg cccccgccat tgctggcgat ggccaccacc tgctccgggg tcaggccatg   4140
ggcctggcac agcaccggca acagccgctg caccgtctcc agagcctgct tgccaccgtc   4200
gtggctggcg atggccacca cctgctgcgg ggtcaggcca tgggcctggc acagcaccgg   4260
caacagccgc tgcaccgtct caagcgcctg cttgccaccg tcgtggctgg cgatggccac   4320
cacctgctcc ggggtcaggc catgggcctg gcacagcacc ggcaacagcc gctggacagt   4380
ctccagcgcc tgcttgccac cattattgct ggcgatggcc accacctgct ccggggtcag   4440
gccatgggcc tggcacagca ccggcaacaa tcgctgcacc gtctccagcg cctgcttgcc   4500
accaatattg ctggcgatgg ccaccacctg ctccggggtc aggccatggg cctggcacag   4560
caccggaagc agccgctgca ccgtctccag cgcctgcttg ccgccatcgt ggctggcgat   4620
ggccaccacc tgctgcggcg taaggttcag gggagcgcct gtcagggcat tccgccaagc   4680
gtgcacggct tccacggctg tcacgcctcc ccgcttggcg atcttcagca gctggccggt   4740
gtccagctgc agtggagggc ctctcagctc gccggccact gtcagcaagg cctcgagtgc   4800
tctagcgccg gaccactgct tgcccacgcc cacgatagcc tcgtgtgtgg cctcgggcag   4860
ggcggcgatc atgtcctggt acttcacggc cacggttccc agagcggcag gatgctggct   4920
cagggccacg atgtgagcgt gggtaaagcc gtggcccacg agggcttcgt ggtgctgggc   4980
cacggtgctc cgcactttgg gcttgatctt ttcctgctgc tgctggctgt agcccagggt   5040
tctcaggtcc acctgggcgg caggagaggc atcggaaggc tgagcggccc ttcttctggg   5100
ggcgctagcg tggatgccca ctttccgctt tttcttgggt ctagaggcgt agtcaggcac   5160
gtcgtaaggg tagcccatga tatcactagc cagcttgggt ctccctatag tgagtcgtat   5220
taatttcgat aagccagtaa gcagtgggtt ctctagttag ccagagagct ctgcttatat   5280
agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac   5340
attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt   5400
ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa   5460
accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt   5520
cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa   5580
tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta   5640
aatagtccac ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg   5700
tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg   5760
taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt   5820
actattaata actagtcaat aatcaatgtc aacgcgtata tctggcccgt acatcgcgaa   5880
gagtc                                                               5885


<210>  25

<211>  19
<212>  DNA
<213>  artificial sequence

<220>
<223>  TALEN T4/5L target sequence

<400>  25
tctcattttc catacagtc                                                  19


<210>  26

<211>  5885
<212>  DNA
<213>  artificial sequence

<220>
<223>  TALEN T4/5L plasmid sequence

<400>  26
aagaacatgt gagcaaaagg ccagcaaaag cccaggaacc gtaaaaaggc cgcgttgctg     60
gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag    120
aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc    180
gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg    240
ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt    300
cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc    360
ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc    420
actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg    480
tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca    540
gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc    600
ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat    660
cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt    720
ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt    780
tttagcacgt gctattattg aagcatttat cagggttatt gtctcatgag cggatacata    840
tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg    900
ccacctgtat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga    960
aattgtaagc gttaataatt cagaagaact cgtcaagaag gcgatagaag gcgatgcgct   1020
gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa   1080
gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca   1140
gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc   1200
aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgctcgcc ttgagcctgg   1260
cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa   1320
gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg   1380
ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt   1440
tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca   1500
gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg   1560
tggccagcca cgatagccgc gctgcctcgt cttgcagttc attcagggca ccggacaggt   1620
cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag   1680
agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg   1740
gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt   1800
gatcagagct tgatcccctg cgccatcaga tccttggcgg cgagaaagcc atccagttta   1860
ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg   1920
ctgtccataa aaccgcccag tagaagccat agagcccacc gcatccccag catgcctgct   1980
attttcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca gaatagaatg   2040
acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga cagtgggagt   2100
ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg gctggcaact   2160
agaaggcaca gtcgaggctg atcagcgggt ttaaacgggc ccttaaaagt ttatctcgcc   2220
gttattaaat ttccgtctca cttcctctaa ggttaatgtg ccggctttaa tcatttctcc   2280
accaattaaa agctcttcta cactaagaac agctccatta caattagtga tatgatttaa   2340
tcgtgtaagc tgagctttgt agtttccttt aaagtgacca ctcacaaata aaaacttaaa   2400
ttccgttaca gaagatggat agactttcca ccattcatta gggttgatat gtttgtttcg   2460
tgtttgattt tcttcgacat atcgttgcat ttcatctgct tggccaattg gcagattata   2520
acctccgcta taagctttag tatccacgat cacaccgtaa tcaataggag atccgacagt   2580
ataaattgct ccgtccggtt tccttgatcc acccaaatgt ttacctctat atccataaac   2640
tttcataaaa aattccatta ccttcatttc aagaattcta tcctgagtgg aatttctggc   2700
aatttcaatt aattcaatat attcatgagg cacatatttc aatttatgac gaagttcaga   2760
tttcttctcc tccagttcac ttttgactag ttgggatcct gtcagggcgg ccagagctgg   2820
gtcgggtctg ctcagctggg ccacaataga ttccagggcg ggtctgcctc cgtcgtggct   2880
ggcaatggcc acgacctgct ggggtgtcag gccatgggcc tggcacagca ccggcaacag   2940
cgcctgcacc gtctccagcg cctgcttgcc gccgccattg ctggcgatgg ccaccacctg   3000
ctgcggggtc aggccatggg cctggcacag caccggcaac agccgctgca ccgtctccag   3060
cgcctgcttg cccccattat tgctggcgat ggccaccacc tgctccgggg tcaggccatg   3120
ggcctggcac agcaccggca acagccgctg caccgtctcc agagcctgct tgccaccaat   3180
attgctggcg atggccacca cctgctccgg ggtcaggcca tgggcctggc acagcaccgg   3240
caacagccgc tgcaccgtct caagcgcctg cttgccaccg tcgtggctgg cgatggccac   3300
cacctgctcc ggggtcaggc catgggcctg gcacagcacc ggcaacagcc gctggacagt   3360
ctccagcgcc tgcttgccac caatattgct ggcgatggcc accacctgct ccggggtcag   3420
gccatgggcc tggcacagca ccggcaacaa tcgctgcacc gtctccagcg cctgcttgcc   3480
accgccattg ctggcgatgg ccaccacctg ctgcggggtc aggccatggg cctggcacag   3540
caccggaagc agccgctgca ccgtctccag cgcctgcttg ccaccaatat tgctggcgat   3600
cgctaccacc tgctggggtg tcaggccatg ggcctggcac agcaccggca acagccgctg   3660
caccgtctcc agcgcctgct tgccaccgtc gtggctggcg atggctacca cctgttgcgg   3720
ggtcaggcca tgggcctggc acagcaccgg caacagccgc tgcaccgtct ccagcgcctg   3780
cttgccaccg tcgtggctgg cgattgccac cacctgctcc ggggtcaggc catgggcctg   3840
gcacagcacc ggcaacagcg cctgcaccgt ctccagcgcc tgcttgccac cgccattgct   3900
tgcgatggcc accacctgct ccggggtcag gccatgggcc tggcacagca ccggcaacag   3960
cgcctgcacc gtctccagcg cctgcttgcc gccgccattg ctggcgatgg ccaccacctg   4020
ctgcggggtc aggccatggg cctggcacag caccggcaac agccgctgca ccgtctccag   4080
cgcctgcttg cccccgccat tgctggcgat ggccaccacc tgctccgggg tcaggccatg   4140
ggcctggcac agcaccggca acagccgctg caccgtctcc agagcctgct tgccaccgcc   4200
attgctggcg atggccacca cctgctccgg ggtcaggcca tgggcctggc acagcaccgg   4260
caacagccgc tgcaccgtct caagcgcctg cttgccacca atattgctgg cgatggccac   4320
cacctgctcc ggggtcaggc catgggcctg gcacagcacc ggcaacagcc gctggacagt   4380
ctccagcgcc tgcttgccac cgtcgtggct ggcgatggcc accacctgct ccggggtcag   4440
gccatgggcc tggcacagca ccggcaacaa tcgctgcacc gtctccagcg cctgcttgcc   4500
accgccattg ctggcgatgg ccaccacctg ctgcggggtc aggccatggg cctggcacag   4560
caccggaagc agccgctgca ccgtctccag cgcctgcttg ccgccatcgt ggctggcgat   4620
ggccaccacc tgctgcggcg taaggttcag gggagcgcct gtcagggcat tccgccaagc   4680
gtgcacggct tccacggctg tcacgcctcc ccgcttggcg atcttcagca gctggccggt   4740
gtccagctgc agtggagggc ctctcagctc gccggccact gtcagcaagg cctcgagtgc   4800
tctagcgccg gaccactgct tgcccacgcc cacgatagcc tcgtgtgtgg cctcgggcag   4860
ggcggcgatc atgtcctggt acttcacggc cacggttccc agagcggcag gatgctggct   4920
cagggccacg atgtgagcgt gggtaaagcc gtggcccacg agggcttcgt ggtgctgggc   4980
cacggtgctc cgcactttgg gcttgatctt ttcctgctgc tgctggctgt agcccagggt   5040
tctcaggtcc acctgggcgg caggagaggc atcggaaggc tgagcggccc ttcttctggg   5100
ggcgctagcg tggatgccca ctttccgctt tttcttgggt ctagaggcgt agtcaggcac   5160
gtcgtaaggg tagcccatga tatcactagc cagcttgggt ctccctatag tgagtcgtat   5220
taatttcgat aagccagtaa gcagtgggtt ctctagttag ccagagagct ctgcttatat   5280
agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac   5340
attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt   5400
ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa   5460
accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt   5520
cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa   5580
tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta   5640
aatagtccac ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg   5700
tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg   5760
taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt   5820
actattaata actagtcaat aatcaatgtc aacgcgtata tctggcccgt acatcgcgaa   5880
gagtc                                                               5885


<210>  27

<211>  19
<212>  DNA
<213>  artificial sequence

<220>
<223>  TALEN T4R target sequence

<400>  27
tttaatgtct ggaaattct                                                  19


<210>  28

<211>  5885
<212>  DNA
<213>  artificial sequence

<220>
<223>  TALEN T4R plasmid sequence

<400>  28
aagaacatgt gagcaaaagg ccagcaaaag cccaggaacc gtaaaaaggc cgcgttgctg     60
gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag    120
aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc    180
gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg    240
ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt    300
cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc    360
ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc    420
actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg    480
tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca    540
gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc    600
ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat    660
cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt    720
ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt    780
tttagcacgt gctattattg aagcatttat cagggttatt gtctcatgag cggatacata    840
tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg    900
ccacctgtat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga    960
aattgtaagc gttaataatt cagaagaact cgtcaagaag gcgatagaag gcgatgcgct   1020
gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa   1080
gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca   1140
gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc   1200
aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgctcgcc ttgagcctgg   1260
cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa   1320
gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg   1380
ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt   1440
tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca   1500
gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg   1560
tggccagcca cgatagccgc gctgcctcgt cttgcagttc attcagggca ccggacaggt   1620
cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag   1680
agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg   1740
gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt   1800
gatcagagct tgatcccctg cgccatcaga tccttggcgg cgagaaagcc atccagttta   1860
ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg   1920
ctgtccataa aaccgcccag tagaagccat agagcccacc gcatccccag catgcctgct   1980
attttcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca gaatagaatg   2040
acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga cagtgggagt   2100
ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg gctggcaact   2160
agaaggcaca gtcgaggctg atcagcgggt ttaaacgggc ccttaaaagt ttatctcgcc   2220
gttattaaat ttccgtctca cttcctctaa ggttaatgtg ccggctttaa tcatttctcc   2280
accaattaaa agctcttcta cactaagaac agctccatta caattagtga tatgatttaa   2340
tcgtgtaagc tgagctttgt agtttccttt aaagtgacca ctcacaaata aaaacttaaa   2400
ttccgttaca gaagatggat agactttcca ccattcatta gggttgatat gtttgtttcg   2460
tgtttgattt tcttcgacat atcgttgcat ttcatctgct tggccaattg gcagattata   2520
acctccgcta taagctttag tatccacgat cacaccgtaa tcaataggag atccgacagt   2580
ataaattgct ccgtccggtt tccttgatcc acccaaatgt ttacctctat atccataaac   2640
tttcataaaa aattccatta ccttcatttc aagaattcta tcctgagtgg aatttctggc   2700
aatttcaatt aattcaatat attcatgagg cacatatttc aatttatgac gaagttcaga   2760
tttcttctcc tccagttcac ttttgactag ttgggatcct gtcagggcgg ccagagctgg   2820
gtcgggtctg ctcagctggg ccacaataga ttccagggcg ggtctgcctc cgccgttgct   2880
ggcaatggcc acgacctgct ggggtgtcag gccatgggcc tggcacagca ccggcaacag   2940
cgcctgcacc gtctccagcg cctgcttgcc gccgtcgtgg ctggcgatgg ccaccacctg   3000
ctgcggggtc aggccatggg cctggcacag caccggcaac agccgctgca ccgtctccag   3060
cgcctgcttg cccccgccat tgctggcgat ggccaccacc tgctccgggg tcaggccatg   3120
ggcctggcac agcaccggca acagccgctg caccgtctcc agagcctgct tgccaccgcc   3180
attgctggcg atggccacca cctgctccgg ggtcaggcca tgggcctggc acagcaccgg   3240
caacagccgc tgcaccgtct caagcgcctg cttgccacca atattgctgg cgatggccac   3300
cacctgctcc ggggtcaggc catgggcctg gcacagcacc ggcaacagcc gctggacagt   3360
ctccagcgcc tgcttgccac caatattgct ggcgatggcc accacctgct ccggggtcag   3420
gccatgggcc tggcacagca ccggcaacaa tcgctgcacc gtctccagcg cctgcttgcc   3480
accaatattg ctggcgatgg ccaccacctg ctccggggtc aggccatggg cctggcacag   3540
caccggaagc agccgctgca ccgtctccag cgcctgcttg ccaccattat tgctggcgat   3600
ggctaccacc tgctggggtg tcaggccatg ggcctggcac agcaccggca acagccgctg   3660
caccgtctcc agcgcctgct tgccaccatt attgctggcg atggctacca cctgttgcgg   3720
ggtcaggcca tgggcctggc acagcaccgg caacagccgc tgcaccgtct ccagcgcctg   3780
cttgccaccg ccattgctgg cgatggccac cacctgctcc ggggtcaggc catgggcctg   3840
gcacagcacc ggcaacagcg cctgcaccgt ctccagcgcc tgcttgccac cgtcgtggct   3900
tgcgatggcc accacctgct ccggggtcag gccatgggcc tggcacagca ccggcaacag   3960
cgcctgcacc gtctccagcg cctgcttgcc gccgccattg ctggcgatgg ccaccacctg   4020
ctgcggggtc aggccatggg cctggcacag caccggcaac agccgctgca ccgtctccag   4080
cgcctgcttg cccccattat tgctggcgat ggccaccacc tgctccgggg tcaggccatg   4140
ggcctggcac agcaccggca acagccgctg caccgtctcc agagcctgct tgccaccgcc   4200
attgctggcg atggccacca cctgctccgg ggtcaggcca tgggcctggc acagcaccgg   4260
caacagccgc tgcaccgtct caagcgcctg cttgccacca atattgctgg cgatggccac   4320
cacctgctcc ggggtcaggc catgggcctg gcacagcacc ggcaacagcc gctggacagt   4380
ctccagcgcc tgcttgccac caatattgct ggcgatggcc accacctgct ccggggtcag   4440
gccatgggcc tggcacagca ccggcaacaa tcgctgcacc gtctccagcg cctgcttgcc   4500
accgccattg ctggcgatgg ccaccacctg ctgcggggtc aggccatggg cctggcacag   4560
caccggaagc agccgctgca ccgtctccag cgcctgcttg ccaccgccat tgctggcgat   4620
ggccaccacc tgctgcggcg taaggttcag gggagcgcct gtcagggcat tccgccaagc   4680
gtgcacggct tccacggctg tcacgcctcc ccgcttggcg atcttcagca gctggccggt   4740
gtccagctgc agtggagggc ctctcagctc gccggccact gtcagcaagg cctcgagtgc   4800
tctagcgccg gaccactgct tgcccacgcc cacgatagcc tcgtgtgtgg cctcgggcag   4860
ggcggcgatc atgtcctggt acttcacggc cacggttccc agagcggcag gatgctggct   4920
cagggccacg atgtgagcgt gggtaaagcc gtggcccacg agggcttcgt ggtgctgggc   4980
cacggtgctc cgcactttgg gcttgatctt ttcctgctgc tgctggctgt agcccagggt   5040
tctcaggtcc acctgggcgg caggagaggc atcggaaggc tgagcggccc ttcttctggg   5100
ggcgctagcg tggatgccca ctttccgctt tttcttgggt ctagaggcgt agtcaggcac   5160
gtcgtaaggg tagcccatga tatcactagc cagcttgggt ctccctatag tgagtcgtat   5220
taatttcgat aagccagtaa gcagtgggtt ctctagttag ccagagagct ctgcttatat   5280
agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac   5340
attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt   5400
ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa   5460
accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt   5520
cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa   5580
tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta   5640
aatagtccac ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg   5700
tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg   5760
taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt   5820
actattaata actagtcaat aatcaatgtc aacgcgtata tctggcccgt acatcgcgaa   5880
gagtc                                                               5885


<210>  29

<211>  19
<212>  DNA
<213>  artificial sequence

<220>
<223>  TALEN T5R target sequence

<400>  29
taatgtctgg aaattcttc                                                  19


<210>  30

<211>  5894
<212>  DNA
<213>  artificial sequence

<220>
<223>  TALEN T5R plasmid sequence

<400>  30
aagaacatgt gagcaaaagg ccagcaaaag cccaggaacc gtaaaaaggc cgcgttgctg     60
gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag    120
aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc    180
gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg    240
ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt    300
cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc    360
ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc    420
actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg    480
tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca    540
gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc    600
ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat    660
cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt    720
ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt    780
tttagcacgt gctattattg aagcatttat cagggttatt gtctcatgag cggatacata    840
tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg    900
ccacctgtat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga    960
aattgtaagc gttaataatt cagaagaact cgtcaagaag gcgatagaag gcgatgcgct   1020
gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa   1080
gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca   1140
gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc   1200
aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgctcgcc ttgagcctgg   1260
cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa   1320
gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg   1380
ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt   1440
tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca   1500
gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg   1560
tggccagcca cgatagccgc gctgcctcgt cttgcagttc attcagggca ccggacaggt   1620
cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag   1680
agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg   1740
gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt   1800
gatcagagct tgatcccctg cgccatcaga tccttggcgg cgagaaagcc atccagttta   1860
ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg   1920
ctgtccataa aaccgcccag tagaagccat agagcccacc gcatccccag catgcctgct   1980
attttcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca gaatagaatg   2040
acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga cagtgggagt   2100
ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg gctggcaact   2160
agaaggcaca gtcgaggctg atcagcgggt ttaaacgggc ccttaaaagt ttatctcgcc   2220
gttattaaat ttccgtctca cttcctctaa ggttaatgtg ccggctttaa tcatttctcc   2280
accaattaaa agctcttcta cactaagaac agctccatta caattagtga tatgatttaa   2340
tcgtgtaagc tgagctttgt agtttccttt aaagtgacca ctcacaaata aaaacttaaa   2400
ttccgttaca gaagatggat agactttcca ccattcatta gggttgatat gtttgtttcg   2460
tgtttgattt tcttcgacat atcgttgcat ttcatctgct tggccaattg gcagattata   2520
acctccgcta taagctttag tatccacgat cacaccgtaa tcaataggag atccgacagt   2580
ataaattgct ccgtccggtt tccttgatcc acccaaatgt ttacctctat atccataaac   2640
tttcataaaa aattccatta ccttcatttc aagaattcta tcctgagtgg aatttctggc   2700
aatttcaatt aattcaatat attcatgagg cacatatttc aatttatgac gaagttcaga   2760
tttcttctcc tccagttcac ttttgactag ttgggatcct gtcagggcgg ccagagctgg   2820
gtcgggtctg ctcagctggg ccacaataga ttccagggcg ggtctgcctc cgtcgtggct   2880
ggcaatggcc acgacctgct ggggtgtcag gccatgggcc tggcacagca ccggcaacag   2940
cgcctgcacc gtctccagcg cctgcttgcc gccgccattg ctggcgatgg ccaccacctg   3000
ctgcggggtc aggccatggg cctggcacag caccggcaac agccgctgca ccgtctccag   3060
cgcctgcttg cccccgccat tgctggcgat ggccaccacc tgctccgggg tcaggccatg   3120
ggcctggcac agcaccggca acagccgctg caccgtctcc aaagcctgct tgccaccgtc   3180
gtggctggcg atggccacca cctgctgcgg ggtcaggcca tgggcctggc acagcaccgg   3240
caacagccgc tgcaccgtct caagcgcctg cttgccaccg ccattgctgg cgatggccac   3300
cacctgctcc ggggtcaggc catgggcctg gcacagcacc ggcaacagcc gctggacagt   3360
ctccagcgcc tgcttgccac cgccattgct ggcgatggcc accacctgct ccggggtcag   3420
gccatgggcc tggcacagca ccggcaacaa tcgctgcacc gtctccagcg cctgcttgcc   3480
accaatattg ctggcgatgg ccaccacctg ctccggggtc aggccatggg cctggcacag   3540
caccggaagc agccgctgca ccgtctccag cgcctgcttg ccaccaatat tgctggcgat   3600
cgctaccacc tgctggggtg tcaggccatg ggcctggcac agcaccggca acagccgctg   3660
caccgtctcc agcgcctgct tgccaccaat attgctggcg atggctacca cctgttgcgg   3720
ggtcaggcca tgggcctggc acagcaccgg caacagccgc tgcaccgtct ccagcgcctg   3780
cttgccacca ttattgctgg cgattgccac cacctgctcc ggggtcaggc catgggcctg   3840
gcacagcacc ggcaacagcg cctgcaccgt ctccagcgcc tgcttgccac cattattgct   3900
tgcgatggcc accacctgct ccggggtcag gccatgggcc tggcacagca ccggcaacag   3960
cgcctgcacc gtctccagcg cctgcttgcc gccgccattg ctggcgatgg ccaccacctg   4020
ctgcggggtc aggccatggg cctggcacag caccggcaac agccgctgca ccgtctccag   4080
cgcctgcttg cccccgtcgt ggctggcgat ggccaccacc tgctccgggg tcaggccatg   4140
ggcctggcac agcaccggca acagccgctg caccgtctcc agagcctgct tgccaccgcc   4200
attgctggcg atggccacca cctgctccgg ggtcaggcca tgggcctggc acagcaccgg   4260
caacagccgc tgcaccgtct caagcgcctg cttgccacca ttattgctgg cgatggccac   4320
cacctgctcc ggggtcaggc catgggcctg gcacagcacc ggcaacagcc gctggacagt   4380
ctccagcgcc tgcttgccac cgccattgct ggcgatggcc accacctgct ccggggtcag   4440
gccatgggcc tggcacagca ccggcaacaa tcgctgcacc gtctccagcg cctgcttgcc   4500
accaatattg ctggcgatgg ccaccacctg ctccggggtc aggccatggg cctggcacag   4560
caccggaagc agccgctgca ccgtctccag cgcctgcttg ccaccaatat tgctggcgat   4620
ggccaccacc tgctgcggcg taaggtcagg gaggttcagg ggagcgcctg tcagggcatt   4680
ccgccaagcg tgcacggctt ccacggctgt cacgcctccc cgcttggcga tcttcagcag   4740
ctggccggtg tccagctgca gtggagggcc tctcagctcg ccggccactg tcagcaaggc   4800
ctcgagtgct ctagcgccgg accactgctt gcccacgccc acgatagcct cgtgtgtggc   4860
ctcgggcagg gcggcgatca tgtcctggta cttcacggcc acggttccca gagcggcagg   4920
atgctggctc agggccacga tgtgagcgtg ggtaaagccg tggcccacga gggcttcgtg   4980
gtgctgggcc acggtgctcc gcactttggg cttgatcttt tcctgctgct gctggctgta   5040
gcccagggtt ctcaggtcca cctgggcggc aggagaggca tcggaaggct gagcggccct   5100
tcttctgggg gcgctagcgt ggatgcccac tttccgcttt ttcttgggtc tagaggcgta   5160
gtcaggcacg tcgtaagggt agcccatgat atcactagcc agcttgggtc tccctatagt   5220
gagtcgtatt aatttcgata agccagtaag cagtgggttc tctagttagc cagagagctc   5280
tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt   5340
tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt   5400
caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt   5460
actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag   5520
taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat   5580
tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag   5640
tttaccgtaa atagtccacc cattgacgtc aatggaaagt ccctattggc gttactatgg   5700
gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc   5760
catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg   5820
taattgatta ctattaataa ctagtcaata atcaatgtca acgcgtatat ctggcccgta   5880
catcgcgaag agtc                                                     5894


<210>  31

<211>  19
<212>  DNA
<213>  artificial sequence

<220>
<223>  TALEN T6L target sequence

<400>  31
ttcattacac ctgcagctc                                                  19


<210>  32

<211>  6201
<212>  DNA
<213>  artificial sequence

<220>
<223>  TALEN T6L plasmid sequence

<400>  32
aagaacatgt gagcaaaagg ccagcaaaag cccaggaacc gtaaaaaggc cgcgttgctg     60
gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag    120
aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc    180
gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg    240
ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt    300
cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc    360
ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc    420
actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg    480
tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca    540
gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc    600
ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat    660
cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt    720
ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt    780
tttagcacgt gctattattg aagcatttat cagggttatt gtctcatgag cggatacata    840
tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg    900
ccacctgtat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga    960
aattgtaagc gttaataatt cagaagaact cgtcaagaag gcgatagaag gcgatgcgct   1020
gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa   1080
gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca   1140
gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc   1200
aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgctcgcc ttgagcctgg   1260
cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa   1320
gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg   1380
ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt   1440
tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca   1500
gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg   1560
tggccagcca cgatagccgc gctgcctcgt cttgcagttc attcagggca ccggacaggt   1620
cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag   1680
agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg   1740
gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt   1800
gatcagagct tgatcccctg cgccatcaga tccttggcgg cgagaaagcc atccagttta   1860
ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg   1920
ctgtccataa aaccgcccag tagaagccat agagcccacc gcatccccag catgcctgct   1980
attgtcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca gaatagaatg   2040
acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga cagtgggagt   2100
ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg gctggcaact   2160
agaaggcaca gtcgaggctg atcagcgggt ttaaacgggc cctctagact cgaggcggcc   2220
gcgctcttct tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt   2280
tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt   2340
tttttttttc tgcaggcaat gaaaataaat gttttttatt aggcagaatc cagatgctca   2400
aggcccttca taatatcccc cagtttagta gttggactta gggaacaaag gaacctttaa   2460
tagaaattgg acagcaagaa agcgagcggt accgatcgct taaaagttta tctcgccgtt   2520
attaaatttc cgtctcactt cctctaaggt taatgtgccg gctttaatca tttctccacc   2580
aattaaaagc tcttctacac taagaacagc tccattacaa ttagtgatat gatttaatcg   2640
tgtaagctga gctttgtagt ttcctttaaa gtgaccactc acaaataaaa acttaaattc   2700
cgttacagaa gatggataga ctttccacca ttcattaggg ttgatatgtt tgtttcgtgt   2760
ttgattttct tcgacatatc gttgcatttc atctgcttgg ccaattggca gattataacc   2820
tccgctataa gctttagtat ccacgatcac accgtaatca ataggagatc cgacagtata   2880
aattgctccg tccggtttcc ttgatccacc caaatgttta cctctatatc cataaacttt   2940
cataaaaaat tccattacct tcatttcaag aattctatcc tgagtggaat ttctggcaat   3000
ttcaattaat tcaatatatt catgaggcac atatttcaat ttatgacgaa gttcagattt   3060
cttctcctcc agttcacttt tgactagttg ggatcctgtc agggcggcca gagctgggtc   3120
gggtctgctc agctgggcca caatagattc cagggcaggt cttccaccat cgtgtgaagc   3180
gatcgcaacc acttgttggg gtgtgagtcc atgatcttgg cagagcactg gcaggagtcg   3240
ttgaactgtc tcaagtgctt gcttgccccc gccatttgaa gctatagcga ctacctgttc   3300
aggggttagg ccgtggtctt gacagaggac gggaagtaac ctctggacag tttcgagggc   3360
ctgctttcca ccatcgtgac tagcaatagc aaccacttga tctggtgtga gtccgtgcgc   3420
ctggcacaac accggcagaa gggcctggac cgtttcaagc gcttgcttgc ccccattgtt   3480
attcgctatt gctaccacct gtgctggcgt gagcccatga gcctggcaaa gaaccggaag   3540
aagccgttgg acagtctcaa gtgcctgctt gcctccgatg ttggacgcaa tcgccaccac   3600
ttggtccggt gtcaacccgt gatcttgaca aagtactggg agcaatcgtt gtacggtttc   3660
cagggcctgt ttcccaccat cgtggctggc gatagctacg acttgttgag gagtaaggcc   3720
atggtcctga cataagacag gaagtaatcg ttgcactgtc tccaaggctt gtttgccacc   3780
attgttagat gcaatcgcta caacttgctc tggggttaaa ccatgcgctt ggcatagtac   3840
gggtaacaat ctttggacag tctctagtgc ttgtttacct ccgccattag atgcgattgc   3900
cactacctga tcgggcgtca gtccatgagc ttgacatagg actggcaaca gtgcttgtac   3960
tgtttcgaga gcttgcttgc ccccatcgtg gttggcgatg gcaacgacct gggccggggt   4020
gaggccatga tcctggcata acacaggcaa gagcctctgg actgtctcaa gcgcctgttt   4080
gccgccatcg tgggaagcaa tggctacgac ctggtccgga gttagaccgt ggtcctgaca   4140
gagtaccggg agcaatcgct gaactgtttc taatgcctgt tttcctccga tgttgcttgc   4200
tatggcaact acttgctggg gggttagccc gtgagcttgg cacaaaactg gaagaagtcg   4260
ctgtacggtt tcaagagcct gcttccctcc atcgtggctg gcgatggcca caacctgctc   4320
aggcgtcaag ccgtgggctt gacacaaaac aggaaggagt ctctgcacag tttccagagc   4380
ttgtttcccg ccgatgttgg aggcaattgc aaccacctga tctggcgtaa gtccgtgatc   4440
ctggcagagg accggcaata acgcctggac agtctccaaa gcctgtttac ccccgccatt   4500
gttcgctatc gctactactt gagcgggtgt taacccatgg tcttggcaca gaacgggcaa   4560
caacctttga accgtttcta gggcttgttt gcccccgcca ttactggcta tcgcgacaac   4620
ttggtcaggt gtcagaccgt gagcctgaca cagcacgggg agcaggcgtt gcacagtctc   4680
aagggcttgc ttaccgccga tgttacttgc tattgcgaca acctgttggg gggtgaggcc   4740
atgcgcctga caaagtacag gcagcaatct ctgaacagtt tccagcgcct gcttcccccc   4800
atcgtggctc gcgatcgcga cgacttgctc cggagtcaat ccatgatctt ggcagagcac   4860
tggaaggagt cgttgaactg tctcaagtgc ttgcttgccc ccgccatttg aagctatagc   4920
gactacctga tcaggcgtaa ggttcagggg agcgcctgtc agggcattcc gccaagcgtg   4980
cacggcttcc acggctgtca cgcctccccg cttggcgatc ttcagcagct ggccggtgtc   5040
cagctgcagt ggagggcctc tcagctcgcc ggccactgtc agcaaggcct cgagtgctct   5100
agcgccggac cactgcttgc ccacgcccac gatagcctcg tgtgtggcct cgggcagggc   5160
ggcgatcatg tcctggtact tcacggccac ggttcccaga gcggcaggat gctggctcag   5220
ggccacgatg tgagcgtggg taaagccgtg gcccacgagg gcttcgtggt gctgggccac   5280
ggtgctccgc actttgggct tgatcttttc ctgctgctgc tggctgtagc ccagggttct   5340
caggtccacc tgggcggcag gagaggcatc ggaaggctga gcggcccttc ttctgggggc   5400
gctagcgtgg atgcccactt tccgcttttt cttgggtcta gaggcgtagt caggcacgtc   5460
gtaagggtag cccatggtgg cgaccggtta cggaaccacg gcttgccagc ttgggtctcc   5520
ctatagtgag tcgtattaat ttcgataagc cagtaagcag tgggttctct agttagccag   5580
agagctctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg   5640
gcggagttgt tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca   5700
ttgacgtcaa tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca   5760
ttgatgtact gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac   5820
tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta   5880
ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag   5940
tgggcagttt accgtaaata gtccacccat tgacgtcaat ggaaagtccc tattggcgtt   6000
actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca   6060
ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat   6120
gaccccgtaa ttgattacta ttaataacta gtcaataatc aatgtcaacg cgtatatctg   6180
gcccgtacat cgcgaagagt c                                             6201


<210>  33

<211>  19
<212>  DNA
<213>  artificial sequence

<220>
<223>  TALEN T6R target sequence

<400>  33
ttccagaatt gatactgac                                                  19


<210>  34

<211>  6205
<212>  DNA

<220>
<223>  TALEN T6R plasmid sequence

<400>  34
aagaacatgt gagcaaaagg ccagcaaaag cccaggaacc gtaaaaaggc cgcgttgctg     60
gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag    120
aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc    180
gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg    240
ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt    300
cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc    360
ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc    420
actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg    480
tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca    540
gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc    600
ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat    660
cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt    720
ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt    780
tttagcacgt gctattattg aagcatttat cagggttatt gtctcatgag cggatacata    840
tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg    900
ccacctgtat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga    960
aattgtaagc gttaataatt cagaagaact cgtcaagaag gcgatagaag gcgatgcgct   1020
gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa   1080
gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca   1140
gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc   1200
aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgctcgcc ttgagcctgg   1260
cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa   1320
gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg   1380
ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt   1440
tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca   1500
gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg   1560
tggccagcca cgatagccgc gctgcctcgt cttgcagttc attcagggca ccggacaggt   1620
cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag   1680
agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg   1740
gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt   1800
gatcagagct tgatcccctg cgccatcaga tccttggcgg cgagaaagcc atccagttta   1860
ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg   1920
ctgtccataa aaccgcccag tagaagccat agagcccacc gcatccccag catgcctgct   1980
attgtcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca gaatagaatg   2040
acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga cagtgggagt   2100
ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg gctggcaact   2160
agaaggcaca gtcgaggctg atcagcgggt ttaaacgggc cctctagact cgaggcggcc   2220
gcgctcttct tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt   2280
tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt   2340
tttttttttc tgcaggcaat gaaaataaat gttttttatt aggcagaatc cagatgctca   2400
aggcccttca taatatcccc cagtttagta gttggactta gggaacaaag gaacctttaa   2460
tagaaattgg acagcaagaa agcgagcggt accgatcgtc gacttaaaag tttatctcgc   2520
cgttattaaa tttccgtctc acttcctcta aggttaatgt gccggcttta atcatttctc   2580
caccaattaa aagctcttct acactaagaa cagctccatt acaattagtg atatgattta   2640
atcgtgtaag ctgagctttg tagtttcctt taaagtgacc actcacaaat aaaaacttaa   2700
attccgttac agaagatgga tagactttcc accattcatt agggttgata tgtttgtttc   2760
gtgtttgatt ttcttcgaca tatcgttgca tttcatctgc ttggccaatt ggcagattat   2820
aacctccgct ataagcttta gtatccacga tcacaccgta atcaatagga gatccgacag   2880
tataaattgc tccgtccggt ttccttgatc cacccaaatg tttacctcta tatccataaa   2940
ctttcataaa aaattccatt accttcattt caagaattct atcctgagtg gaatttctgg   3000
caatttcaat taattcaata tattcatgag gcacatattt caatttatga cgaagttcag   3060
atttcttctc ctccagttca cttttgacta gttgggatcc tgtcagggcg gccagagctg   3120
ggtcgggtct gctcagctgg gccacaatag attccagggc aggtcttcca ccatcgtgtg   3180
aagcgatcgc aaccacttgt tggggtgtga gtccatgatc ttggcagagc actggcagga   3240
gtcgttgaac tgtctcaagt gcttgcttgc ccccgatgtt tgaagctata gcgactacct   3300
gttcaggggt taggccgtgg tcttgacaga ggacgggaag taacctctgg acagtttcga   3360
gggcctgctt tccaccattg ttactagcaa tagcaaccac ttgatctggt gtgagtccgt   3420
gcgcctggca caacaccggc agaagggcct ggaccgtttc aagcgcttgc ttgcccccgc   3480
cattattcgc tattgctacc acctgtgctg gcgtgagccc atgagcctgg caaagaaccg   3540
gaagaagccg ttggacagtc tcaagtgcct gcttgcctcc atcgtgggac gcaatcgcca   3600
ccacttggtc cggtgtcaac ccgtgatctt gacaaagtac tgggagcaat cgttgtacgg   3660
tttccagggc ctgtttccca ccgatgttgc tggcgatagc tacgacttgt tgaggagtaa   3720
ggccatggtc ctgacataag acaggaagta atcgttgcac tgtctccaag gcttgtttgc   3780
caccgccatt agatgcaatc gctacaactt gctctggggt taaaccatgc gcttggcata   3840
gtacgggtaa caatctttgg acagtctcta gtgcttgttt acctccgatg ttagatgcga   3900
ttgccactac ctgatcgggc gtcagtccat gagcttgaca taggactggc aacagtgctt   3960
gtactgtttc gagagcttgc ttgcccccat tgttgttggc gatggcaacg acctgggccg   4020
gggtgaggcc atgatcctgg cataacacag gcaagagcct ctggactgtc tcaagcgcct   4080
gtttgccgcc gccattggaa gcaatggcta cgacctggtc cggagttaga ccgtggtcct   4140
gacagagtac cgggagcaat cgctgaactg tttctaatgc ctgttttcct ccgccattgc   4200
ttgctatggc aactacttgc tggggggtta gcccgtgagc ttggcacaaa actggaagaa   4260
gtcgctgtac ggtttcaaga gcctgcttcc ctccgatgtt gctggcgatg gccacaacct   4320
gctcaggcgt caagccgtgg gcttgacaca aaacaggaag gagtctctgc acagtttcca   4380
gagcttgttt cccgccgatg ttggaggcaa ttgcaaccac ctgatctggc gtaagtccgt   4440
gatcctggca gaggaccggc aataacgcct ggacagtctc caaagcctgt ttacccccat   4500
tgttgttcgc tatcgctact acttgagcgg gtgttaaccc atggtcttgg cacagaacgg   4560
gcaacaacct ttgaaccgtt tctagggctt gtttgccccc gatgttactg gctatcgcga   4620
caacttggtc aggtgtcaga ccgtgagcct gacacagcac ggggagcagg cgttgcacag   4680
tctcaagggc ttgcttaccg ccatcgtgac ttgctattgc gacaacctgt tggggggtga   4740
ggccatgcgc ctgacaaagt acaggcagca atctctgaac agtttccagc gcctgcttcc   4800
ccccatcgtg gctcgcgatc gcgacgactt gctccggagt caatccatga tcttggcaga   4860
gcactggaag gagtcgttga actgtctcaa gtgcttgctt gcccccgcca tttgaagcta   4920
tagcgactac ctgatcaggc gtaaggttca ggggagcgcc tgtcagggca ttccgccaag   4980
cgtgcacggc ttccacggct gtcacgcctc cccgcttggc gatcttcagc agctggccgg   5040
tgtccagctg cagtggaggg cctctcagct cgccggccac tgtcagcaag gcctcgagtg   5100
ctctagcgcc ggaccactgc ttgcccacgc ccacgatagc ctcgtgtgtg gcctcgggca   5160
gggcggcgat catgtcctgg tacttcacgg ccacggttcc cagagcggca ggatgctggc   5220
tcagggccac gatgtgagcg tgggtaaagc cgtggcccac gagggcttcg tggtgctggg   5280
ccacggtgct ccgcactttg ggcttgatct tttcctgctg ctgctggctg tagcccaggg   5340
ttctcaggtc cacctgggcg gcaggagagg catcggaagg ctgagcggcc cttcttctgg   5400
gggcgctagc gtggatgccc actttccgct ttttcttggg tctagaggcg tagtcaggca   5460
cgtcgtaagg gtagcccatg gtggcgaccg gttacggaac cacggcttgc cagcttgggt   5520
ctccctatag tgagtcgtat taatttcgat aagccagtaa gcagtgggtt ctctagttag   5580
ccagagagct ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa   5640
tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact   5700
cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg   5760
cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat   5820
gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca   5880
tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc   5940
caagtgggca gtttaccgta aatagtccac ccattgacgt caatggaaag tccctattgg   6000
cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca   6060
gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac   6120
taatgacccc gtaattgatt actattaata actagtcaat aatcaatgtc aacgcgtata   6180
tctggcccgt acatcgcgaa gagtc                                         6205


<210>  35

<211>  12
<212>  DNA
<213>  artificial sequence

<220>
<223>  ZFN L target sequence

<400>  35
gtcatcctca tc                                                         12


<210>  36

<211>  6280
<212>  DNA
<213>  artificial sequence

<220>
<223>  ZFN L plasmid sequence

<400>  36
gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg     60
ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg    120
cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc    180
ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt    240
gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata    300
tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc    360
cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc    420
attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt    480
atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt    540
atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca    600
tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg    660
actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc    720
aaaatcaacg gcactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg    780
gtagccgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg    840
cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc    900
tccgcggccg ggaacggtgc attggaacgc ggattccccg tgccaagagt gacgtaagta    960
ccgcctatag agtctatggc ccaccccctt ggcttcgtta gaacgcggct acaattaata   1020
cataacctta tgtatcatac acatacgatt taggtgacac tatagaataa catccacttt   1080
gcctttctct ccacaggtgt ctaggatggg ctacccttac gacgtgcctg actacgcctc   1140
tagacccaag aaaaagcgga aagtgggcat ccacgctagc cctgccgcca tggctgagag   1200
gcccttccag tgccggatct gcatgcggaa cttcagcgac cggtccaacc tgagccggca   1260
catccggacc cacaccggcg agaagccctt cgcctgcgac atctgcggcc ggaagttcgc   1320
catcagcagc aacctgaaca gccacaccaa gatccacacc ggcagccaga aacctttcca   1380
gtgcagaatc tgtatgagaa acttcagccg cagcgacaac ctggcaagac acatcagaac   1440
acacacgggc gagaaaccgt ttgcatgtga tatctgtggg cgaaagttcg ccacctccgg   1500
caatctgacc cggcacacaa agatccacct gaggggatcc caactagtca agagcgagct   1560
ggaagaaaag aagagcgaac tgcgccacaa gctgaaatac gtgcctcacg agtacatcga   1620
actgatcgaa atcgcccgca acagcaccca ggaccgcatc ctggaaatga aagtgatgga   1680
attcttcatg aaggtgtacg gctaccgcgg caagcacctg ggcggcagcc gcaagcctga   1740
cggcgccatc tacaccgtgg gcagccctat cgactacggc gtgatcgtgg acaccaaggc   1800
ctacagcggc ggctacaacc tgcctatcgg ccaggccgac gaaatggaac gctacgtgga   1860
agaaaaccag acccgcaaca agcacgccaa ccctaacgaa tggtggaagg tgtaccctag   1920
cagcgtgacc gaattcaagt tcctgttcgt gagcggccac ttcaagggca actacaaggc   1980
ccagctgacc cgcctgaacc acatcaccaa ctgcaacggc gccgtgctgt ccgtggaaga   2040
actgctgatc ggcggcgaaa tgattaaggc cgggaccctg accctggaag aagtgcgccg   2100
caagttcaac aacggcgaaa tcaactttta agggcccttc gaaggtaagc ctatccctaa   2160
ccctctcctc ggtctcgatt ctacgcgtac cggtcatcat caccatcacc attgagttta   2220
aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc   2280
ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga   2340
ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca   2400
ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc   2460
tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg   2520
tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc   2580
cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg   2640
ctttccccgt caagctctaa atcggggcat ccctttaggg ttccgattta gtgctttacg   2700
gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg   2760
atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt   2820
ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt   2880
ggggatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta   2940
attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc aggcaggcag   3000
aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc   3060
cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc   3120
cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg   3180
ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca   3240
gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg   3300
tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc   3360
ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagccttt gtctcaagaa   3420
gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac   3480
tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta   3540
tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg   3600
gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc   3660
ccctgcggac ggtgtcgaca ggtgcttctc gatctgcatc ctgggatcaa agcgatagtg   3720
aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat   3780
gtgtgggagg gctaagcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt   3840
cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg   3900
ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt   3960
tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc   4020
atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt   4080
ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt   4140
gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa   4200
agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc   4260
tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag   4320
aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt   4380
cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga   4440
atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg   4500
taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa   4560
aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt   4620
tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct   4680
gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct   4740
cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc   4800
cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt   4860
atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc   4920
tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat   4980
ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa   5040
acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa   5100
aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga   5160
aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct   5220
tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga   5280
cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc   5340
catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg   5400
ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat   5460
aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat   5520
ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg   5580
caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc   5640
attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa   5700
agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc   5760
actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt   5820
ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag   5880
ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt   5940
gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag   6000
atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac   6060
cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc   6120
gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca   6180
gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg   6240
ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc                         6280


<210>  37

<211>  12
<212>  DNA
<213>  artificial sequence

<220>
<223>  ZFN R target sequence

<400>  37
cttttgcagt tt                                                         12


<210>  38

<211>  6277
<212>  DNA
<213>  artificial sequence

<220>
<223>  ZFN R plasmid sequence

<400>  38
gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta     60
aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata    120
ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc    180
ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga    240
agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct    300
tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg    360
tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta    420
ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat    480
gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt    540
acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga    600
tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga    660
gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga    720
actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc    780
aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc    840
cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg    900
tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat    960
cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata   1020
tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct   1080
ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga   1140
ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg   1200
cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc   1260
aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct   1320
agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc   1380
tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt   1440
ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg   1500
cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca   1560
ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag   1620
ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag   1680
tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg   1740
gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg   1800
gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac   1860
cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt   1920
gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat   1980
tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc   2040
aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc   2100
tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca   2160
tgattacgcc aagctctagc tagaggtcga cggtatacag acatgataag atacattgat   2220
gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt   2280
gatgctattg ctttatttgt aaccattata agctgcaata aacaagttgg ggtgggcgaa   2340
gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc ggaaaacgat   2400
tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgta gcacgtgtca   2460
gtcctgctcc tcggccacga agtgcttagc cctcccacac ataaccagag ggcagcaatt   2520
cacgaatccc aactgccgtc ggctgtccat cactgtcctt cactatcgct ttgatcccag   2580
gatgcagatc gagaagcacc tgtcgacacc gtccgcaggg gctcaagatg cccctgttct   2640
catttccgat cgcgacgata caagtcaggt tgccagctgc cgcagcagca gcagtgccca   2700
gcaccacgag ttctgcacaa ggtcccccag taaaatgata tacattgaca ccagtgaaga   2760
tgcggccgtc gctagagaga gctgcgctgg cgacgctgta gtcttcagag atggggatgc   2820
tgttgattgt agccgttgct ctttcaatga gggtggattc ttcttgagac aaaggcttgg   2880
ccatggttta gttcctcacc ttgtcgtatt atactatgcc gatatactat gccgatgatt   2940
aattgtcaac acgtgctgat cagatccgaa aatggatata caagctcccg ggagcttttt   3000
gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggcaga   3060
ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc   3120
ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga   3180
ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca   3240
cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgcct ggggagcctg   3300
gggactttcc acaccctaac tgacacacat tccacagaat taattcgcgt taaatttttg   3360
ttaaatcagc tcatttttta accaataggc cgaaatcccc aaaatccctt ataaatcaaa   3420
agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa   3480
gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg   3540
tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa   3600
ccctaaaggg atgccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa   3660
ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct   3720
gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc   3780
cctagagccc cagctggttc tttccgcctc agaagccata gagcccaccg catccccagc   3840
atgcctgcta ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc caccccccag   3900
aatagaatga cacctactca gacaatgcga tgcaatttcc tcattttatt aggaaaggac   3960
agtgggagtg gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg   4020
ctggcaacta gaaggcacag tcgaggctga tcagcgggtt taaactcaat ggtgatggtg   4080
atgatgaccg gtacgcgtag aatcgagacc gaggagaggg ttagggatag gcttaccttc   4140
gaagggccct taaaagttga tttcgccgtt gttgaacttg cggcgcactt cttccagggt   4200
cagggtgccg gccttaatca tttcgccgcc gatcagcagt tcttccacgg acagcacggc   4260
gccgttgcag ttggtcacgt ggttcaggcg ggtcagctgg gccttgtagt tgcccttgaa   4320
gtggccgctc acgaacagga acttgaattc ggtcacgctg ctagggtaca ccttccacca   4380
ttcgttaggg ttgatgtgct tgttgcgggt ctggttttct ttcacgtagc gctgcatttc   4440
gtcggcctgg ccgataggca ggttgtagcc gccgctgtag gccttggtgt ccacgatcac   4500
gccgtagtcg atagggctgc ccacggtgta gatggcgccg tcaggcttgc ggctgccgcc   4560
caggtgcttg ccgcggtagc cgtacacctt catgaagaat tccatcactt tcatttccag   4620
gatgcggtcc tgggtgctgt tgcgggcgat ttcgatcagt tcgatgtact cgtgaggcac   4680
gtatttcagc ttgtggcgca gttcgctctt cttttcttcc agctcgctct tgactagttg   4740
ggatcccctc aggtggatct ttgtgtgggt ggtccggtgg ttccgctggg caaactttct   4800
cccacatata tcgcaggcaa acggtttctc ccctgtgtgt gttctgatgt gctcggacag   4860
cacgtcgctg cggctgaagt ttctcataca tattctgcat tggaaaggct tttcgcctgt   4920
gtggatcttg gtgtgcacct ggaggttgat tttctgggcg aacttccggc cgcagatgtc   4980
gcaggcgaag ggcttctcgc cggtgtgggt ccggatgtgc acgctcaggt tgtcggatct   5040
gctgaagttc cgcatgcaga tccggcactg gaagggcctc tcagccatgg cggcagggct   5100
agcgtggatg cccactttcc gctttttctt gggtctagag gcgtagtcag gcacgtcgta   5160
agggtagccc atcctagaca cctgtggaga gaaaggcaaa gtggatgtta ttctatagtg   5220
tcacctaaat cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa   5280
cgaagccaag ggggtgggcc atagactcta taggcggtac ttacgtcact cttggcacgg   5340
ggaatccgcg ttccaatgca ccgttcccgg ccgcggaggc tggatcggtc ccggtgtctt   5400
ctatggaggt caaaacagcg tggatggcgt ctccaggcga tctgacggtt cactaaacga   5460
gctctgctta tatagacctc ccaccgtaca cggctaccgc ccatttgcgt caatggggcg   5520
gagttgttac gacattttgg aaagtgccgt tgattttggt gccaaaacaa actcccattg   5580
acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg   5640
atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc   5700
caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg   5760
tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg   5820
gcagtttacc gtaaatagtc cacccattga cgtcaatgga aagtccctat tggcgttact   5880
atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc   5940
gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac   6000
cccgtaattg attactatta ataactagtc aataatcaat gtcaacgcgt atatctggcc   6060
cgtacatcgc gaagcagcgc aaaacgccta accctaagca gattcttcat gcaattgtcg   6120
gtcaagcctt gccttgttgt agcttaaatt ttgctcgcgc actactcagc gacctccaac   6180
acacaagcag ggagcagata ctggcttaac tatgcggcat cagagcagat tgtactgaga   6240
gtcgaccata ggggatcggg agatctcccg atccgtc                            6277


<210>  39

<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  gRNA#3

<400>  39
cctgcctccg ctctactcac                                                 20


<210>  40

<211>  32
<212>  DNA
<213>  artificial sequence

<220>
<223>  Delta32

<400>  40
gtcagtatca attctggaag aatttccaga ca                                   32


<210>  41

<211>  11
<212>  DNA
<213>  artificial sequence

<220>
<223>  MegaTAL-T target sequence

<400>  41
gtccttctcc t                                                          11


<210>  42

<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  MegaTAL- M cleavage sequence

<400>  42
cttccaggaa ttctttggcc tg                                              22


<210>  43

<211>  3378
<212>  DNA
<213>  artificial sequence

<220>
<223>  MegaTAL polynucleotide sequence

<400>  43
atgggatcct gcaggtatcc atatgatgtc ccagattatg cgccacctaa gaagaaacgc     60
aaagtcgtgg atctacgcac gctcggctac agtcagcagc agcaagagaa gatcaaaccg    120
aaggtgcgtt cgacagtggc gcagcaccac gaggcactgg tgggccatgg gtttacacac    180
gcgcacatcg ttgcgctcag ccaacacccg gcagcgttag ggaccgtcgc tgtcacgtat    240
cagcacataa tcacggcgtt gccagaggcg acacacgaag acatcgttgg cgtcggcaaa    300
cagtggtccg gcgcacgcgc cctggaggcc ttgctcacgg atgcggggga gttgagaggt    360
ccgccgttac agttggacac aggccaactt gtgaagattg caaaacgtgg cggcgtgacc    420
gcaatggagg cagtgcatgc atcgcgcaat gcactgacgg gtgcccccct gaacctgacc    480
ccggaccaag tggtggctat cgccagcaac aatggcggca agcaagcgct cgaaacggtg    540
cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct    600
atcgccagca acggtggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg    660
ctgtgccagg accatggcct gactccggac caagtggtgg ctatcgccag ccacgatggc    720
ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc    780
ctgactccgg accaagtggt ggctatcgcc agccacgatg gcggcaagca agcgctcgaa    840
acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg    900
gtggctatcg ccagcaacgg tggcggcaag caagcgctcg aaacggtgca gcggctgttg    960
ccggtgctgt gccaggacca tggcctgacc ccggaccaag tggtggctat cgccagcaac   1020
ggtggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac   1080
catggcctga ctccggacca agtggtggct atcgccagcc acgatggcgg caagcaagcg   1140
ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct gaccccggac   1200
caagtggtgg ctatcgccag caacggtggc ggcaagcaag cgctcgaaac ggtgcagcgg   1260
ctgttgccgg tgctgtgcca ggaccatggc ctgactccgg accaagtggt ggctatcgcc   1320
agccacgatg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc   1380
caggaccatg gcctgactcc ggaccaagtg gtggctatcg ccagccacga tggcggcaag   1440
caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc   1500
ccggaccaag tggtggctat cgccagcaac ggtggcggca agcaagcgct cgaaagcatt   1560
gtggcccagc tgagccggcc tgatccggcg ttggccgcgt tgaccaacga ccacctcgtc   1620
gccttggcct gcctcggcgg acgtcctgcc atggatgcag tgaaaaaggg attgccgcac   1680
gcgccggaat tgatcagaag agtcaatcgc cgtattggcg aacgcacgtc ccatcgcgtt   1740
gcgatatcta gagtgggagg aagctcgcgc agagagtcca tcaacccatg gattctgact   1800
ggtttcgctg atgccgaagc atccttcatg ctaagaatcc gtagcgcgaa caaccggtca   1860
gtagggtact acactgacct ggtattcgaa atcactctgc acaacaagga caaatcgatt   1920
ctggagaata tccagtcgac ttggaaggtc ggcacaatca acaaccgagg cgacggcacc   1980
gtcagactga gcgtcacgcg ccacgaagat ttgaaagtga ttatcgacca cttcgagaaa   2040
tatccgctga ttacccagaa attgggcgat tacaagttgt ttaaacaggc attcagcgtc   2100
atggagaaca aagaacatct taaggagaat gggattaagg agctcgtacg aatcaaagct   2160
aagatgaatt ggggtctcaa tgacgaattg aaaaaagcat ttccagagaa catcagcaaa   2220
gagcgccccc ttatcaataa gaacattccg aatctcaaat ggctggctgg attcacatct   2280
ggtgaaggca cattctacgt gcacctagca aagcccaccc gccagaacaa ggtacaggtg   2340
cgactgaggt tcataatcgg ccagcacatc cgagacaaga acctgatgaa ttcattgata   2400
acatacctag gctgtggtac gatccaggag aagaacaggt ctaagggcag tatgctccac   2460
ttcatagtaa ctaaattcag cgatatcaac gacaagatca ttccggtatt ccaggaaaat   2520
actctgattg gcgtcaaact cgaggacttt gaagattggt gcaaggttgc caaattgatc   2580
gaagagaaga aacacctgac cgaatccggt ttggatgaga ttaagaaaat caagctgaac   2640
atgaacaaag gtcgtgtctt cgctagcacc ggttctgagc cacctcgggc tgagaccttt   2700
gtattcctgg acctagaagc cactgggctc ccaaacatgg accctgagat tgcagagata   2760
tccctttttg ctgttcaccg ctcttccctg gagaacccag aacgggatga ttctggttcc   2820
ttggtgctgc cccgtgttct ggacaagctc acactgtgca tgtgcccgga gcgccccttt   2880
actgccaagg ccagtgagat tactggtttg agcagcgaaa gcctgatgca ctgcgggaag   2940
gctggtttca atggcgctgt ggtaaggaca ctgcagggct tcctaagccg ccaggagggc   3000
cccatctgcc ttgtggccca caatggcttc gattatgact tcccactgct gtgcacggag   3060
ctacaacgtc tgggtgccca tctgccccaa gacactgtct gcctggacac actgcctgca   3120
ttgcggggcc tggaccgtgc tcacagccac ggcaccaggg ctcaaggccg caaaagctac   3180
agcctggcca gtctcttcca ccgctacttc caggctgaac ccagtgctgc ccattcagca   3240
gaaggtgatg tgcacaccct gcttctgatc ttcctgcatc gtgctcctga gctgctcgcc   3300
tgggcagatg agcaggcccg cagctgggct catattgagc ccatgtacgt gccacctgat   3360
ggtccaagcc tcgaagcc                                                 3378


<210>  44

<211>  3378
<212>  DNA
<213>  artificial sequence

<220>
<223>  MegaTAL + Trex2 polynucleotide sequence

<400>  44
atgggatcct gcaggtatcc atatgatgtc ccagattatg cgccacctaa gaagaaacgc     60
aaagtcgtgg atctacgcac gctcggctac agtcagcagc agcaagagaa gatcaaaccg    120
aaggtgcgtt cgacagtggc gcagcaccac gaggcactgg tgggccatgg gtttacacac    180
gcgcacatcg ttgcgctcag ccaacacccg gcagcgttag ggaccgtcgc tgtcacgtat    240
cagcacataa tcacggcgtt gccagaggcg acacacgaag acatcgttgg cgtcggcaaa    300
cagtggtccg gcgcacgcgc cctggaggcc ttgctcacgg atgcggggga gttgagaggt    360
ccgccgttac agttggacac aggccaactt gtgaagattg caaaacgtgg cggcgtgacc    420
gcaatggagg cagtgcatgc atcgcgcaat gcactgacgg gtgcccccct gaacctgacc    480
ccggaccaag tggtggctat cgccagcaac aatggcggca agcaagcgct cgaaacggtg    540
cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct    600
atcgccagca acggtggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg    660
ctgtgccagg accatggcct gactccggac caagtggtgg ctatcgccag ccacgatggc    720
ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc    780
ctgactccgg accaagtggt ggctatcgcc agccacgatg gcggcaagca agcgctcgaa    840
acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg    900
gtggctatcg ccagcaacgg tggcggcaag caagcgctcg aaacggtgca gcggctgttg    960
ccggtgctgt gccaggacca tggcctgacc ccggaccaag tggtggctat cgccagcaac   1020
ggtggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac   1080
catggcctga ctccggacca agtggtggct atcgccagcc acgatggcgg caagcaagcg   1140
ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct gaccccggac   1200
caagtggtgg ctatcgccag caacggtggc ggcaagcaag cgctcgaaac ggtgcagcgg   1260
ctgttgccgg tgctgtgcca ggaccatggc ctgactccgg accaagtggt ggctatcgcc   1320
agccacgatg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc   1380
caggaccatg gcctgactcc ggaccaagtg gtggctatcg ccagccacga tggcggcaag   1440
caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc   1500
ccggaccaag tggtggctat cgccagcaac ggtggcggca agcaagcgct cgaaagcatt   1560
gtggcccagc tgagccggcc tgatccggcg ttggccgcgt tgaccaacga ccacctcgtc   1620
gccttggcct gcctcggcgg acgtcctgcc atggatgcag tgaaaaaggg attgccgcac   1680
gcgccggaat tgatcagaag agtcaatcgc cgtattggcg aacgcacgtc ccatcgcgtt   1740
gcgatatcta gagtgggagg aagctcgcgc agagagtcca tcaacccatg gattctgact   1800
ggtttcgctg atgccgaagc atccttcatg ctaagaatcc gtagcgcgaa caaccggtca   1860
gtagggtact acactgacct ggtattcgaa atcactctgc acaacaagga caaatcgatt   1920
ctggagaata tccagtcgac ttggaaggtc ggcacaatca acaaccgagg cgacggcacc   1980
gtcagactga gcgtcacgcg ccacgaagat ttgaaagtga ttatcgacca cttcgagaaa   2040
tatccgctga ttacccagaa attgggcgat tacaagttgt ttaaacaggc attcagcgtc   2100
atggagaaca aagaacatct taaggagaat gggattaagg agctcgtacg aatcaaagct   2160
aagatgaatt ggggtctcaa tgacgaattg aaaaaagcat ttccagagaa catcagcaaa   2220
gagcgccccc ttatcaataa gaacattccg aatctcaaat ggctggctgg attcacatct   2280
ggtgaaggca cattctacgt gcacctagca aagcccaccc gccagaacaa ggtacaggtg   2340
cgactgaggt tcataatcgg ccagcacatc cgagacaaga acctgatgaa ttcattgata   2400
acatacctag gctgtggtac gatccaggag aagaacaggt ctaagggcag tatgctccac   2460
ttcatagtaa ctaaattcag cgatatcaac gacaagatca ttccggtatt ccaggaaaat   2520
actctgattg gcgtcaaact cgaggacttt gaagattggt gcaaggttgc caaattgatc   2580
gaagagaaga aacacctgac cgaatccggt ttggatgaga ttaagaaaat caagctgaac   2640
atgaacaaag gtcgtgtctt cgctagcacc ggttctgagc cacctcgggc tgagaccttt   2700
gtattcctgg acctagaagc cactgggctc ccaaacatgg accctgagat tgcagagata   2760
tccctttttg ctgttcaccg ctcttccctg gagaacccag aacgggatga ttctggttcc   2820
ttggtgctgc cccgtgttct ggacaagctc acactgtgca tgtgcccgga gcgccccttt   2880
actgccaagg ccagtgagat tactggtttg agcagcgaaa gcctgatgca ctgcgggaag   2940
gctggtttca atggcgctgt ggtaaggaca ctgcagggct tcctaagccg ccaggagggc   3000
cccatctgcc ttgtggccca caatggcttc gattatgact tcccactgct gtgcacggag   3060
ctacaacgtc tgggtgccca tctgccccaa gacactgtct gcctggacac actgcctgca   3120
ttgcggggcc tggaccgtgc tcacagccac ggcaccaggg ctcaaggccg caaaagctac   3180
agcctggcca gtctcttcca ccgctacttc caggctgaac ccagtgctgc ccattcagca   3240
gaaggtgatg tgcacaccct gcttctgatc ttcctgcatc gtgctcctga gctgctcgcc   3300
tgggcagatg agcaggcccg cagctgggct catattgagc ccatgtacgt gccacctgat   3360
ggtccaagcc tcgaagcc                                                 3378



