                         SEQUENCE LISTING

<110>  Ruprecht-Karls-Universitt Heidelberg
 
<120>  Adeno-Associated Virus Library

<130>  UH14326PC

<150>  EP17165906.3
<151>  2017-04-11

<160>  52    

<170>  PatentIn version 3.3

<210>  1
<211>  4
<212>  PRT
<213>  Artificial

<220>
<223>  generic recognition motif, X = any amino acid


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  1

Asn Xaa Xaa Arg 
1               


<210>  2
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  generic recognition motif, X = any amino acid


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (5)..(7)
<223>  Xaa can be any naturally occurring amino acid

<400>  2

Asn Xaa Xaa Arg Xaa Xaa Xaa 
1               5           


<210>  3
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  inserted binding peptide

<400>  3

Cys Asp Cys Arg Gly Asp Cys Phe Cys 
1               5                   


<210>  4
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  inserted binding peptide

<400>  4

Asn Asp Val Arg Ser Ala Asn 
1               5           


<210>  5
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  inserted binding peptide

<400>  5

Asn Asp Val Arg Ala Val Ser 
1               5           


<210>  6
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  inserted binding peptide

<400>  6

Met Pro Leu Gly Ala Ala Gly 
1               5           


<210>  7
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  inserted binding peptide

<400>  7

Asn Tyr Ser Arg Gly Val Asp 
1               5           


<210>  8
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  inserted binding peptide

<400>  8

Asn Glu Ala Arg Val Arg Glu 
1               5           


<210>  9
<211>  11
<212>  PRT
<213>  Artificial

<220>
<223>  binding peptide

<400>  9

Gly Cys Asp Cys Arg Gly Asp Cys Phe Cys Ala 
1               5                   10      


<210>  10
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  binding peptide

<400>  10

Gly Asn Asp Val Arg Ser Ala Asn Ala 
1               5                   


<210>  11
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  binding peptide

<400>  11

Gly Asn Asp Val Arg Ala Val Ser Ala 
1               5                   


<210>  12
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  binding peptide

<400>  12

Gly Met Pro Leu Gly Ala Ala Gly Ala 
1               5                   


<210>  13
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  binding peptide

<400>  13

Gly Asn Tyr Ser Arg Gly Val Asp Ala 
1               5                   


<210>  14
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  binding peptide

<400>  14

Gly Asn Glu Ala Arg Val Arg Glu Ala 
1               5                   


<210>  15
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Variant 1 insertion site AAV1 and AAV6


<220>
<221>  MISC_FEATURE
<222>  (3)..(4)
<223>  insertion site

<400>  15

Ser Thr Asp Pro Ala Thr 
1               5       


<210>  16
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Variant 1 insertion site AAV2


<220>
<221>  MISC_FEATURE
<222>  (3)..(4)
<223>  insertion site

<400>  16

Gly Asn Arg Gln Ala Ala 
1               5       


<210>  17
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Variant 1 insertion site AAV3b


<220>
<221>  MISC_FEATURE
<222>  (3)..(4)
<223>  insertion site

<400>  17

Leu Gln Ser Ser Asn Thr 
1               5       


<210>  18
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Variant 1 insertion site AAV4


<220>
<221>  MISC_FEATURE
<222>  (3)..(4)
<223>  insertion site

<400>  18

Asp Gln Ser Asn Ser Asn 
1               5       


<210>  19
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Variant 1 insertion site AAV5


<220>
<221>  MISC_FEATURE
<222>  (3)..(4)
<223>  insertion site

<400>  19

Asn Gln Ser Ser Thr Thr 
1               5       


<210>  20
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Variant 1 insertion site AAV7


<220>
<221>  MISC_FEATURE
<222>  (3)..(4)
<223>  insertion site

<400>  20

Ala Ala Asn Thr Ala Ala 
1               5       


<210>  21
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Variant 1 insertion site AAV8


<220>
<221>  MISC_FEATURE
<222>  (3)..(4)
<223>  insertion site

<400>  21

Gln Gln Asn Thr Ala Pro 
1               5       


<210>  22
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Variant 1 insertion site AAV9


<220>
<221>  MISC_FEATURE
<222>  (3)..(4)
<223>  insertion site

<400>  22

Ser Ala Gln Ala Gln Ala 
1               5       


<210>  23
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Variant 1 insertion site AAVrh10


<220>
<221>  MISC_FEATURE
<222>  (3)..(4)
<223>  insertion site

<400>  23

Gln Gln Asn Ala Ala Pro 
1               5       


<210>  24
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Variant 1 insertion site AAVpo.1


<220>
<221>  MISC_FEATURE
<222>  (3)..(4)
<223>  insertion site

<400>  24

Asn Gln Asn Ser Asn Thr 
1               5       


<210>  25
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Variant 1 insertion site AAV12


<220>
<221>  MISC_FEATURE
<222>  (3)..(4)
<223>  insertion site

<400>  25

Asn Gln Asn Ala Thr Thr 
1               5       


<210>  26
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Variant 2 insertion site AAV1 and AAV6


<220>
<221>  MISC_FEATURE
<222>  (3)..(4)
<223>  insertion site

<400>  26

Ser Ser Ser Thr Asp Pro 
1               5       


<210>  27
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Variant 2 insertion site AAV2


<220>
<221>  MISC_FEATURE
<222>  (3)..(4)
<223>  insertion site

<400>  27

Arg Gly Asn Arg Gln Ala 
1               5       


<210>  28
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Variant 2 insertion site AAV3b


<220>
<221>  MISC_FEATURE
<222>  (3)..(4)
<223>  insertion site

<400>  28

Ser Ser Asn Thr Ala Pro 
1               5       


<210>  29
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Variant 2 insertion site AAV4


<220>
<221>  MISC_FEATURE
<222>  (3)..(4)
<223>  insertion site

<400>  29

Ser Asn Ser Asn Leu Pro 
1               5       


<210>  30
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Variant 2 insertion site AAV5


<220>
<221>  MISC_FEATURE
<222>  (3)..(4)
<223>  insertion site

<400>  30

Ser Ser Thr Thr Ala Pro 
1               5       


<210>  31
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Variant 2 insertion site AAVpo.1


<220>
<221>  MISC_FEATURE
<222>  (3)..(4)
<223>  insertion site

<400>  31

Asn Ser Asn Thr His Pro 
1               5       


<210>  32
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Variant 2 insertion site AAV12


<220>
<221>  MISC_FEATURE
<222>  (3)..(4)
<223>  insertion site

<400>  32

Asn Ala Thr Thr Ala Pro 
1               5       


<210>  33
<211>  11
<212>  PRT
<213>  Artificial

<220>
<223>  AAV2 wt insertion site


<220>
<221>  MISC_FEATURE
<222>  (5)..(6)
<223>  insertion site

<400>  33

Thr Asn Leu Gln Arg Gly Asn Arg Gln Ala Ala 
1               5                   10      


<210>  34
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  AAV2 wt insertion site encoding

<400>  34
accaacctcc agagaggcaa cagacaagca gct                                  33


<210>  35
<211>  12
<212>  PRT
<213>  Artificial

<220>
<223>  AAV2 NIS


<220>
<221>  MISC_FEATURE
<222>  (7)..(8)
<223>  insertion site

<400>  35

Thr Asn Leu Gln Gly Gln Arg Gly Ala Gln Ala Ala 
1               5                   10          


<210>  36
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  AAV2 NIS encoding

<400>  36
accaacctcc agggccagag aggcgcccag gcggcc                               36


<210>  37
<211>  13
<212>  PRT
<213>  Artificial

<220>
<223>  AAV2 2xA


<220>
<221>  MISC_FEATURE
<222>  (10)..(11)
<223>  insertion site

<400>  37

Thr Asn Leu Gln Arg Gly Asn Ala Ala Ala Ala Ala Arg 
1               5                   10              


<210>  38
<211>  39
<212>  DNA
<213>  Artificial

<220>
<223>  AAV2 2xA encoding

<400>  38
accaacctcc agagaggcaa cgcggcggca gcagccaga                            39


<210>  39
<211>  14
<212>  PRT
<213>  Artificial

<220>
<223>  AAV2 3xA


<220>
<221>  MISC_FEATURE
<222>  (10)..(11)
<223>  insertion site

<400>  39

Thr Asn Leu Gln Arg Gly Asn Ala Ala Ala Ala Ala Ala Arg 
1               5                   10                  


<210>  40
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  AAV2 3xA encoding

<400>  40
accaacctcc agagaggcaa cgcggcggca gcagccgcga ga                        42


<210>  41
<211>  15
<212>  PRT
<213>  Artificial

<220>
<223>  AAV2 4xA


<220>
<221>  MISC_FEATURE
<222>  (10)..(11)
<223>  insertion site

<400>  41

Thr Asn Leu Gln Arg Gly Asn Ala Ala Ala Ala Ala Ala Ala Arg 
1               5                   10                  15  


<210>  42
<211>  45
<212>  DNA
<213>  Artificial

<220>
<223>  AAV2 4xA encoding

<400>  42
accaacctcc agagaggcaa cgcggcggca gcagccgcgg ccaga                     45


<210>  43
<211>  16
<212>  PRT
<213>  Artificial

<220>
<223>  AAV2 wt extended

<400>  43

Thr Asn Leu Gln Arg Gly Asn Arg Gln Ala Ala Thr Ala Asp Val Asn 
1               5                   10                  15      


<210>  44
<211>  25
<212>  PRT
<213>  Artificial

<220>
<223>  AAV2_A2(OIS)

<400>  44

Thr Asn Leu Gln Arg Gly Gln Arg Gly Asn Tyr Ser Arg Gly Val Asp 
1               5                   10                  15      


Ala Gln Ala Ala Thr Ala Asp Val Asn 
            20                  25  


<210>  45
<211>  25
<212>  PRT
<213>  Artificial

<220>
<223>  AAV2_A2(NIS)

<400>  45

Thr Asn Leu Gln Gly Gln Arg Gly Asn Tyr Ser Arg Gly Val Asp Ala 
1               5                   10                  15      


Gln Ala Ala Ala Thr Ala Asp Val Asn 
            20                  25  


<210>  46
<211>  28
<212>  PRT
<213>  Artificial

<220>
<223>  AAV2_A2_2xA(NIS)

<400>  46

Thr Asn Leu Gln Arg Gly Asn Ala Ala Ala Asn Tyr Ser Arg Gly Val 
1               5                   10                  15      


Asp Ala Ala Arg Gln Ala Ala Thr Ala Asp Val Asn 
            20                  25              


<210>  47
<211>  29
<212>  PRT
<213>  Artificial

<220>
<223>  AAV2_A2_3xA(NIS)

<400>  47

Thr Asn Leu Gln Arg Gly Asn Ala Ala Ala Asn Tyr Ser Arg Gly Val 
1               5                   10                  15      


Asp Ala Ala Ala Arg Gln Ala Ala Thr Ala Asp Val Asn 
            20                  25                  


<210>  48
<211>  30
<212>  PRT
<213>  Artificial

<220>
<223>  AAV2_A2_4xA(NIS)

<400>  48

Thr Asn Leu Gln Arg Gly Asn Ala Ala Ala Asn Tyr Ser Arg Gly Val 
1               5                   10                  15      


Asp Ala Ala Ala Ala Arg Gln Ala Ala Thr Ala Asp Val Asn 
            20                  25                  30  


<210>  49
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  inserted binding peptide

<400>  49

Asn Ser Ser Arg Asp Leu Gly 
1               5           


<210>  50
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  inserted binding peptide

<400>  50

Ser Glu Gly Leu Lys Asn Leu 
1               5           


<210>  51
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  inserted binding peptide

<400>  51

Pro Ser Val Pro Arg Pro Pro 
1               5           


<210>  52
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  inserted binding peptide

<400>  52

Asn Phe Thr Arg Leu Ser Ala 
1               5           


