                         SEQUENCE LISTING

<110>  Second Genome, Inc.
       Han, Andrew Wonhee
       Goodyear, Andrew Whitman
       Gujral, Tarunmeet
       DeSantis, Todd Zachary
       Dabbagh, Karim
       Takeuchi, Toshihiko
       Jin, Ye
       Willcoxon, Michi Izumi
       Banas, Stefanie
 
<120>  PROTEINS FOR THE TREATMENT OF EPITHELIAL BARRIER FUNCTION 
       DISORDERS

<130>  SEGE-001/03WO 321077-2031

<150>  US 62/611,334
<151>  2017-12-28

<150>  US 62/607,706
<151>  2017-12-19

<150>  US 62/482,963
<151>  2017-04-07

<160>  34    

<170>  PatentIn version 3.5

<210>  1
<211>  256
<212>  PRT
<213>  Roseburia hominis

<400>  1

Met Lys Arg Leu Val Cys Thr Val Cys Ser Val Leu Leu Cys Ala Gly 
1               5                   10                  15      


Leu Leu Ser Gly Cys Gly Thr Ser Leu Glu Gly Glu Glu Ser Val Val 
            20                  25                  30          


Tyr Val Gly Lys Lys Gly Val Ile Ala Ser Leu Asp Val Glu Thr Leu 
        35                  40                  45              


Asp Gln Ser Tyr Tyr Asp Glu Thr Glu Leu Lys Ser Tyr Val Asp Ala 
    50                  55                  60                  


Glu Val Glu Asp Tyr Thr Ala Glu His Gly Lys Asn Ala Val Lys Val 
65                  70                  75                  80  


Glu Ser Leu Lys Val Glu Asp Gly Val Ala Lys Leu Lys Met Lys Tyr 
                85                  90                  95      


Lys Thr Pro Glu Asp Tyr Thr Ala Phe Asn Gly Ile Glu Leu Tyr Gln 
            100                 105                 110         


Gly Lys Val Val Ala Ser Leu Ala Ala Gly Tyr Val Tyr Asp Gly Glu 
        115                 120                 125             


Phe Ala Arg Val Glu Glu Gly Lys Val Val Gly Ala Ala Thr Lys Gln 
    130                 135                 140                 


Asp Ile Tyr Ser Glu Asp Asp Leu Lys Val Ala Ile Ile Arg Ala Asn 
145                 150                 155                 160 


Thr Asp Val Lys Val Asp Gly Glu Ile Cys Tyr Val Ser Cys Gln Asn 
                165                 170                 175     


Val Lys Leu Thr Gly Lys Asp Ser Val Ser Ile Arg Asp Gly Tyr Tyr 
            180                 185                 190         


Leu Glu Thr Gly Ser Val Thr Ala Ser Val Asp Val Thr Gly Gln Glu 
        195                 200                 205             


Ser Val Gly Thr Glu Gln Leu Ser Gly Thr Glu Gln Met Glu Met Thr 
    210                 215                 220                 


Gly Glu Pro Val Asn Ala Asp Asp Thr Glu Gln Thr Glu Ala Ala Ala 
225                 230                 235                 240 


Gly Asp Gly Ser Phe Glu Thr Asp Val Tyr Thr Phe Ile Val Tyr Lys 
                245                 250                 255     


<210>  2
<211>  768
<212>  DNA
<213>  Roseburia hominis

<400>  2
atgaagagat tagtgtgcac ggtctgcagt gtactgttgt gtgcgggact tctctccgga       60

tgcggtacct cgctggaggg agaggaaagt gtcgtgtacg tgggaaagaa aggcgtgata      120

gcgtcgctgg atgtggagac gctcgatcag tcctactacg atgagacgga actgaagtcc      180

tatgtggatg cagaggtgga agattacacc gcggagcatg gtaaaaatgc agtcaaggtg      240

gagagcctta aggtggaaga cggtgtggcg aagcttaaga tgaagtacaa gacaccggag      300

gattataccg catttaatgg aattgaactc tatcagggga aagtcgttgc ttccctggcg      360

gcaggatacg tctacgacgg ggagttcgcc cgcgtggagg aaggcaaggt tgtgggagct      420

gccacaaaac aggatattta ctctgaggat gatttgaaag ttgccatcat ccgtgccaat      480

acggatgtga aggtggacgg tgagatctgc tatgtctcct gtcagaatgt gaagctgacc      540

ggaaaagaca gtgtgtcgat ccgtgacgga tattatcttg agacgggaag cgtgacggca      600

tccgtggatg tgaccggaca ggagagcgtc gggaccgagc agctttcggg aaccgaacag      660

atggagatga ccggggagcc ggtgaatgcg gatgataccg agcagacaga ggcggcggcc      720

ggtgacggtt cgttcgagac agacgtatat actttcattg tctacaaa                   768


<210>  3
<211>  232
<212>  PRT
<213>  Roseburia hominis

<400>  3

Leu Glu Gly Glu Glu Ser Val Val Tyr Val Gly Lys Lys Gly Val Ile 
1               5                   10                  15      


Ala Ser Leu Asp Val Glu Thr Leu Asp Gln Ser Tyr Tyr Asp Glu Thr 
            20                  25                  30          


Glu Leu Lys Ser Tyr Val Asp Ala Glu Val Glu Asp Tyr Thr Ala Glu 
        35                  40                  45              


His Gly Lys Asn Ala Val Lys Val Glu Ser Leu Lys Val Glu Asp Gly 
    50                  55                  60                  


Val Ala Lys Leu Lys Met Lys Tyr Lys Thr Pro Glu Asp Tyr Thr Ala 
65                  70                  75                  80  


Phe Asn Gly Ile Glu Leu Tyr Gln Gly Lys Val Val Ala Ser Leu Ala 
                85                  90                  95      


Ala Gly Tyr Val Tyr Asp Gly Glu Phe Ala Arg Val Glu Glu Gly Lys 
            100                 105                 110         


Val Val Gly Ala Ala Thr Lys Gln Asp Ile Tyr Ser Glu Asp Asp Leu 
        115                 120                 125             


Lys Val Ala Ile Ile Arg Ala Asn Thr Asp Val Lys Val Asp Gly Glu 
    130                 135                 140                 


Ile Cys Tyr Val Ser Cys Gln Asn Val Lys Leu Thr Gly Lys Asp Ser 
145                 150                 155                 160 


Val Ser Ile Arg Asp Gly Tyr Tyr Leu Glu Thr Gly Ser Val Thr Ala 
                165                 170                 175     


Ser Val Asp Val Thr Gly Gln Glu Ser Val Gly Thr Glu Gln Leu Ser 
            180                 185                 190         


Gly Thr Glu Gln Met Glu Met Thr Gly Glu Pro Val Asn Ala Asp Asp 
        195                 200                 205             


Thr Glu Gln Thr Glu Ala Ala Ala Gly Asp Gly Ser Phe Glu Thr Asp 
    210                 215                 220                 


Val Tyr Thr Phe Ile Val Tyr Lys 
225                 230         


<210>  4
<211>  696
<212>  DNA
<213>  Roseburia hominis

<400>  4
ctggagggag aggaaagtgt cgtgtacgtg ggaaagaaag gcgtgatagc gtcgctggat       60

gtggagacgc tcgatcagtc ctactacgat gagacggaac tgaagtccta tgtggatgca      120

gaggtggaag attacaccgc ggagcatggt aaaaatgcag tcaaggtgga gagccttaag      180

gtggaagacg gtgtggcgaa gcttaagatg aagtacaaga caccggagga ttataccgca      240

tttaatggaa ttgaactcta tcaggggaaa gtcgttgctt ccctggcggc aggatacgtc      300

tacgacgggg agttcgcccg cgtggaggaa ggcaaggttg tgggagctgc cacaaaacag      360

gatatttact ctgaggatga tttgaaagtt gccatcatcc gtgccaatac ggatgtgaag      420

gtggacggtg agatctgcta tgtctcctgt cagaatgtga agctgaccgg aaaagacagt      480

gtgtcgatcc gtgacggata ttatcttgag acgggaagcg tgacggcatc cgtggatgtg      540

accggacagg agagcgtcgg gaccgagcag ctttcgggaa ccgaacagat ggagatgacc      600

ggggagccgg tgaatgcgga tgataccgag cagacagagg cggcggccgg tgacggttcg      660

ttcgagacag acgtatatac tttcattgtc tacaaa                                696


<210>  5
<211>  241
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SG11 expressed in pGEX6 vector and cleaved by Precision protease

<400>  5

Gly Pro Leu Gly Ser Leu Glu Gly Glu Glu Ser Val Val Tyr Val Gly 
1               5                   10                  15      


Lys Lys Gly Val Ile Ala Ser Leu Asp Val Glu Thr Leu Asp Gln Ser 
            20                  25                  30          


Tyr Tyr Asp Glu Thr Glu Leu Lys Ser Tyr Val Asp Ala Glu Val Glu 
        35                  40                  45              


Asp Tyr Thr Ala Glu His Gly Lys Asn Ala Val Lys Val Glu Ser Leu 
    50                  55                  60                  


Lys Val Glu Asp Gly Val Ala Lys Leu Lys Met Lys Tyr Lys Thr Pro 
65                  70                  75                  80  


Glu Asp Tyr Thr Ala Phe Asn Gly Ile Glu Leu Tyr Gln Gly Lys Val 
                85                  90                  95      


Val Ala Ser Leu Ala Ala Gly Tyr Val Tyr Asp Gly Glu Phe Ala Arg 
            100                 105                 110         


Val Glu Glu Gly Lys Val Val Gly Ala Ala Thr Lys Gln Asp Ile Tyr 
        115                 120                 125             


Ser Glu Asp Asp Leu Lys Val Ala Ile Ile Arg Ala Asn Thr Asp Val 
    130                 135                 140                 


Lys Val Asp Gly Glu Ile Cys Tyr Val Ser Cys Gln Asn Val Lys Leu 
145                 150                 155                 160 


Thr Gly Lys Asp Ser Val Ser Ile Arg Asp Gly Tyr Tyr Leu Glu Thr 
                165                 170                 175     


Gly Ser Val Thr Ala Ser Val Asp Val Thr Gly Gln Glu Ser Val Gly 
            180                 185                 190         


Thr Glu Gln Leu Ser Gly Thr Glu Gln Met Glu Met Thr Gly Glu Pro 
        195                 200                 205             


Val Asn Ala Asp Asp Thr Glu Gln Thr Glu Ala Ala Ala Gly Asp Gly 
    210                 215                 220                 


Ser Phe Glu Thr Asp Val Tyr Thr Phe Ile Val Tyr Lys Ala Ala Ala 
225                 230                 235                 240 


Ser 
    


<210>  6
<211>  723
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SG11 expressed in pGEX6 vector and cleaved by Precision protease

<400>  6
gggcccctgg gatccctgga gggagaggaa agtgtcgtgt acgtgggaaa gaaaggcgtg       60

atagcgtcgc tggatgtgga gacgctcgat cagtcctact acgatgagac ggaactgaag      120

tcctatgtgg atgcagaggt ggaagattac accgcggagc atggtaaaaa tgcagtcaag      180

gtggagagcc ttaaggtgga agacggtgtg gcgaagctta agatgaagta caagacaccg      240

gaggattata ccgcatttaa tggaattgaa ctctatcagg ggaaagtcgt tgcttccctg      300

gcggcaggat acgtctacga cggggagttc gcccgcgtgg aggaaggcaa ggttgtggga      360

gctgccacaa aacaggatat ttactctgag gatgatttga aagttgccat catccgtgcc      420

aatacggatg tgaaggtgga cggtgagatc tgctatgtct cctgtcagaa tgtgaagctg      480

accggaaaag acagtgtgtc gatccgtgac ggatattatc ttgagacggg aagcgtgacg      540

gcatccgtgg atgtgaccgg acaggagagc gtcgggaccg agcagctttc gggaaccgaa      600

cagatggaga tgaccgggga gccggtgaat gcggatgata ccgagcagac agaggcggcg      660

gccggtgacg gttcgttcga gacagacgta tatactttca ttgtctacaa agcggccgca      720

tcg                                                                    723


<210>  7
<211>  233
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SG11 with start methionine

<400>  7

Met Leu Glu Gly Glu Glu Ser Val Val Tyr Val Gly Lys Lys Gly Val 
1               5                   10                  15      


Ile Ala Ser Leu Asp Val Glu Thr Leu Asp Gln Ser Tyr Tyr Asp Glu 
            20                  25                  30          


Thr Glu Leu Lys Ser Tyr Val Asp Ala Glu Val Glu Asp Tyr Thr Ala 
        35                  40                  45              


Glu His Gly Lys Asn Ala Val Lys Val Glu Ser Leu Lys Val Glu Asp 
    50                  55                  60                  


Gly Val Ala Lys Leu Lys Met Lys Tyr Lys Thr Pro Glu Asp Tyr Thr 
65                  70                  75                  80  


Ala Phe Asn Gly Ile Glu Leu Tyr Gln Gly Lys Val Val Ala Ser Leu 
                85                  90                  95      


Ala Ala Gly Tyr Val Tyr Asp Gly Glu Phe Ala Arg Val Glu Glu Gly 
            100                 105                 110         


Lys Val Val Gly Ala Ala Thr Lys Gln Asp Ile Tyr Ser Glu Asp Asp 
        115                 120                 125             


Leu Lys Val Ala Ile Ile Arg Ala Asn Thr Asp Val Lys Val Asp Gly 
    130                 135                 140                 


Glu Ile Cys Tyr Val Ser Cys Gln Asn Val Lys Leu Thr Gly Lys Asp 
145                 150                 155                 160 


Ser Val Ser Ile Arg Asp Gly Tyr Tyr Leu Glu Thr Gly Ser Val Thr 
                165                 170                 175     


Ala Ser Val Asp Val Thr Gly Gln Glu Ser Val Gly Thr Glu Gln Leu 
            180                 185                 190         


Ser Gly Thr Glu Gln Met Glu Met Thr Gly Glu Pro Val Asn Ala Asp 
        195                 200                 205             


Asp Thr Glu Gln Thr Glu Ala Ala Ala Gly Asp Gly Ser Phe Glu Thr 
    210                 215                 220                 


Asp Val Tyr Thr Phe Ile Val Tyr Lys 
225                 230             


<210>  8
<211>  699
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SG11 with start methionine

<400>  8
atgttggagg gtgaagagtc tgttgtctat gtgggtaaga aaggtgtgat cgcgtccctg       60

gacgtcgaga ctctggacca gtcttactat gatgaaaccg agctgaagtc gtatgtggac      120

gccgaagttg aggattacac ggccgagcac ggcaaaaatg ccgtcaaagt tgagagcttg      180

aaagttgagg acggcgtggc aaagctgaag atgaaataca agaccccaga ggactacacg      240

gcgttcaatg gtatcgagct gtatcagggc aaagtcgtcg catccctggc agcgggctat      300

gtgtacgacg gtgagtttgc gcgcgtcgaa gaaggcaaag ttgtgggtgc ggctacgaaa      360

caagatatct acagcgaaga tgacctgaaa gtcgcgatta ttcgtgctaa caccgatgtt      420

aaagttgatg gcgagatttg ctacgttagc tgtcaaaacg taaagctgac gggtaaagat      480

agcgtgagca ttcgtgatgg ctattatctg gaaaccggta gcgttacggc gagcgtcgat      540

gttaccggtc aagagagcgt gggtaccgaa cagctgagcg gcaccgaaca gatggaaatg      600

accggtgaac cggttaacgc agacgacacg gaacaaaccg aagccgcggc aggcgacggt      660

agcttcgaga ctgacgtgta cacctttatc gtgtacaag                             699


<210>  9
<211>  246
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SG11 with N-terminal FLAG tag

<400>  9

Met Asp Tyr Lys Asp Asp Asp Asp Lys Gly Ser Ser His Met Leu Glu 
1               5                   10                  15      


Gly Glu Glu Ser Val Val Tyr Val Gly Lys Lys Gly Val Ile Ala Ser 
            20                  25                  30          


Leu Asp Val Glu Thr Leu Asp Gln Ser Tyr Tyr Asp Glu Thr Glu Leu 
        35                  40                  45              


Lys Ser Tyr Val Asp Ala Glu Val Glu Asp Tyr Thr Ala Glu His Gly 
    50                  55                  60                  


Lys Asn Ala Val Lys Val Glu Ser Leu Lys Val Glu Asp Gly Val Ala 
65                  70                  75                  80  


Lys Leu Lys Met Lys Tyr Lys Thr Pro Glu Asp Tyr Thr Ala Phe Asn 
                85                  90                  95      


Gly Ile Glu Leu Tyr Gln Gly Lys Val Val Ala Ser Leu Ala Ala Gly 
            100                 105                 110         


Tyr Val Tyr Asp Gly Glu Phe Ala Arg Val Glu Glu Gly Lys Val Val 
        115                 120                 125             


Gly Ala Ala Thr Lys Gln Asp Ile Tyr Ser Glu Asp Asp Leu Lys Val 
    130                 135                 140                 


Ala Ile Ile Arg Ala Asn Thr Asp Val Lys Val Asp Gly Glu Ile Cys 
145                 150                 155                 160 


Tyr Val Ser Cys Gln Asn Val Lys Leu Thr Gly Lys Asp Ser Val Ser 
                165                 170                 175     


Ile Arg Asp Gly Tyr Tyr Leu Glu Thr Gly Ser Val Thr Ala Ser Val 
            180                 185                 190         


Asp Val Thr Gly Gln Glu Ser Val Gly Thr Glu Gln Leu Ser Gly Thr 
        195                 200                 205             


Glu Gln Met Glu Met Thr Gly Glu Pro Val Asn Ala Asp Asp Thr Glu 
    210                 215                 220                 


Gln Thr Glu Ala Ala Ala Gly Asp Gly Ser Phe Glu Thr Asp Val Tyr 
225                 230                 235                 240 


Thr Phe Ile Val Tyr Lys 
                245     


<210>  10
<211>  738
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SG11 with N-terminal FLAG tag (not codon optimized)

<400>  10
atggactaca aagacgatga cgacaagggc agcagccata tgctggaggg agaggaaagt       60

gtcgtgtacg tgggaaagaa aggcgtgata gcgtcgctgg atgtggagac gctcgatcag      120

tcctactacg atgagacgga actgaagtcc tatgtggatg cagaggtgga agattacacc      180

gcggagcatg gtaaaaatgc agtcaaggtg gagagcctta aggtggaaga cggtgtggcg      240

aagcttaaga tgaagtacaa gacaccggag gattataccg catttaatgg aattgaactc      300

tatcagggga aagtcgttgc ttccctggcg gcaggatacg tctacgacgg ggagttcgcc      360

cgcgtggagg aaggcaaggt tgtgggagct gccacaaaac aggatattta ctctgaggat      420

gatttgaaag ttgccatcat ccgtgccaat acggatgtga aggtggacgg tgagatctgc      480

tatgtctcct gtcagaatgt gaagctgacc ggaaaagaca gtgtgtcgat ccgtgacgga      540

tattatcttg agacgggaag cgtgacggca tccgtggatg tgaccggaca ggagagcgtc      600

gggaccgagc agctttcggg aaccgaacag atggagatga ccggggagcc ggtgaatgcg      660

gatgataccg agcagacaga ggcggcggcc ggtgacggtt cgttcgagac agacgtatat      720

actttcattg tctacaaa                                                    738


<210>  11
<211>  233
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial variant of SG11  (C147V, C151S)

<400>  11

Met Leu Glu Gly Glu Glu Ser Val Val Tyr Val Gly Lys Lys Gly Val 
1               5                   10                  15      


Ile Ala Ser Leu Asp Val Glu Thr Leu Asp Gln Ser Tyr Tyr Asp Glu 
            20                  25                  30          


Thr Glu Leu Lys Ser Tyr Val Asp Ala Glu Val Glu Asp Tyr Thr Ala 
        35                  40                  45              


Glu His Gly Lys Asn Ala Val Lys Val Glu Ser Leu Lys Val Glu Asp 
    50                  55                  60                  


Gly Val Ala Lys Leu Lys Met Lys Tyr Lys Thr Pro Glu Asp Tyr Thr 
65                  70                  75                  80  


Ala Phe Asn Gly Ile Glu Leu Tyr Gln Gly Lys Val Val Ala Ser Leu 
                85                  90                  95      


Ala Ala Gly Tyr Val Tyr Asp Gly Glu Phe Ala Arg Val Glu Glu Gly 
            100                 105                 110         


Lys Val Val Gly Ala Ala Thr Lys Gln Asp Ile Tyr Ser Glu Asp Asp 
        115                 120                 125             


Leu Lys Val Ala Ile Ile Arg Ala Asn Thr Asp Val Lys Val Asp Gly 
    130                 135                 140                 


Glu Ile Val Tyr Val Ser Ser Gln Asn Val Lys Leu Thr Gly Lys Asp 
145                 150                 155                 160 


Ser Val Ser Ile Arg Asp Gly Tyr Tyr Leu Glu Thr Gly Ser Val Thr 
                165                 170                 175     


Ala Ser Val Asp Val Thr Gly Gln Glu Ser Val Gly Thr Glu Gln Leu 
            180                 185                 190         


Ser Gly Thr Glu Gln Met Glu Met Thr Gly Glu Pro Val Asn Ala Asp 
        195                 200                 205             


Asp Thr Glu Gln Thr Glu Ala Ala Ala Gly Asp Gly Ser Phe Glu Thr 
    210                 215                 220                 


Asp Val Tyr Thr Phe Ile Val Tyr Lys 
225                 230             


<210>  12
<211>  699
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial variant of SG11  (C147V, C151S) (codon optimized)

<400>  12
atgttggagg gtgaagagtc tgttgtctat gtgggtaaga aaggtgtgat cgcgtccctg       60

gacgtcgaga ctctggacca gtcttactat gatgaaaccg agctgaagtc gtatgtggac      120

gccgaagttg aggattacac ggccgagcac ggcaaaaatg ccgtcaaagt tgagagcttg      180

aaagttgagg acggcgtggc aaagctgaag atgaaataca agaccccaga ggactacacg      240

gcgttcaatg gtatcgagct gtatcagggc aaagtcgtcg catccctggc agcgggctat      300

gtgtacgacg gtgagtttgc gcgcgtcgaa gaaggcaaag ttgtgggtgc ggctacgaaa      360

caagatatct acagcgaaga tgacctgaaa gtcgcgatta ttcgtgctaa caccgatgtt      420

aaagttgatg gcgagattgt gtacgttagc agccaaaacg taaagctgac gggtaaagat      480

agcgtgagca ttcgtgatgg ctattatctg gaaaccggta gcgttacggc gagcgtcgat      540

gttaccggtc aagagagcgt gggtaccgaa cagctgagcg gcaccgaaca gatggaaatg      600

accggtgaac cggttaacgc agacgacacg gaacaaaccg aagccgcggc aggcgacggt      660

agcttcgaga ctgacgtgta cacctttatc gtgtacaag                             699


<210>  13
<211>  233
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial variant of SG11  (G84D, C147V, C151S)

<400>  13

Met Leu Glu Gly Glu Glu Ser Val Val Tyr Val Gly Lys Lys Gly Val 
1               5                   10                  15      


Ile Ala Ser Leu Asp Val Glu Thr Leu Asp Gln Ser Tyr Tyr Asp Glu 
            20                  25                  30          


Thr Glu Leu Lys Ser Tyr Val Asp Ala Glu Val Glu Asp Tyr Thr Ala 
        35                  40                  45              


Glu His Gly Lys Asn Ala Val Lys Val Glu Ser Leu Lys Val Glu Asp 
    50                  55                  60                  


Gly Val Ala Lys Leu Lys Met Lys Tyr Lys Thr Pro Glu Asp Tyr Thr 
65                  70                  75                  80  


Ala Phe Asn Asp Ile Glu Leu Tyr Gln Gly Lys Val Val Ala Ser Leu 
                85                  90                  95      


Ala Ala Gly Tyr Val Tyr Asp Gly Glu Phe Ala Arg Val Glu Glu Gly 
            100                 105                 110         


Lys Val Val Gly Ala Ala Thr Lys Gln Asp Ile Tyr Ser Glu Asp Asp 
        115                 120                 125             


Leu Lys Val Ala Ile Ile Arg Ala Asn Thr Asp Val Lys Val Asp Gly 
    130                 135                 140                 


Glu Ile Val Tyr Val Ser Ser Gln Asn Val Lys Leu Thr Gly Lys Asp 
145                 150                 155                 160 


Ser Val Ser Ile Arg Asp Gly Tyr Tyr Leu Glu Thr Gly Ser Val Thr 
                165                 170                 175     


Ala Ser Val Asp Val Thr Gly Gln Glu Ser Val Gly Thr Glu Gln Leu 
            180                 185                 190         


Ser Gly Thr Glu Gln Met Glu Met Thr Gly Glu Pro Val Asn Ala Asp 
        195                 200                 205             


Asp Thr Glu Gln Thr Glu Ala Ala Ala Gly Asp Gly Ser Phe Glu Thr 
    210                 215                 220                 


Asp Val Tyr Thr Phe Ile Val Tyr Lys 
225                 230             


<210>  14
<211>  699
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial variant of SG11  (G84D, C147V, C151S) (codon 
       optimized)

<400>  14
atgttggagg gtgaagagtc tgttgtctat gtgggtaaga aaggtgtgat cgcgtccctg       60

gacgtcgaga ctctggacca gtcttactat gatgaaaccg agctgaagtc gtatgtggac      120

gccgaagttg aggattacac ggccgagcac ggcaaaaatg ccgtcaaagt tgagagcttg      180

aaagttgagg acggcgtggc aaagctgaag atgaaataca agaccccaga ggactacacg      240

gcgttcaatg acatcgagct gtatcagggc aaagtcgtcg catccctggc agcgggctat      300

gtgtacgacg gtgagtttgc gcgcgtcgaa gaaggcaaag ttgtgggtgc ggctacgaaa      360

caagatatct acagcgaaga tgacctgaaa gtcgcgatta ttcgtgctaa caccgatgtt      420

aaagttgatg gcgagattgt gtacgttagc agccaaaacg taaagctgac gggtaaagat      480

agcgtgagca ttcgtgatgg ctattatctg gaaaccggta gcgttacggc gagcgtcgat      540

gttaccggtc aagagagcgt gggtaccgaa cagctgagcg gcaccgaaca gatggaaatg      600

accggtgaac cggttaacgc agacgacacg gaacaaaccg aagccgcggc aggcgacggt      660

agcttcgaga ctgacgtgta cacctttatc gtgtacaag                             699


<210>  15
<211>  233
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial variant of SG11  (N83S, C147V, C151S)

<400>  15

Met Leu Glu Gly Glu Glu Ser Val Val Tyr Val Gly Lys Lys Gly Val 
1               5                   10                  15      


Ile Ala Ser Leu Asp Val Glu Thr Leu Asp Gln Ser Tyr Tyr Asp Glu 
            20                  25                  30          


Thr Glu Leu Lys Ser Tyr Val Asp Ala Glu Val Glu Asp Tyr Thr Ala 
        35                  40                  45              


Glu His Gly Lys Asn Ala Val Lys Val Glu Ser Leu Lys Val Glu Asp 
    50                  55                  60                  


Gly Val Ala Lys Leu Lys Met Lys Tyr Lys Thr Pro Glu Asp Tyr Thr 
65                  70                  75                  80  


Ala Phe Ser Gly Ile Glu Leu Tyr Gln Gly Lys Val Val Ala Ser Leu 
                85                  90                  95      


Ala Ala Gly Tyr Val Tyr Asp Gly Glu Phe Ala Arg Val Glu Glu Gly 
            100                 105                 110         


Lys Val Val Gly Ala Ala Thr Lys Gln Asp Ile Tyr Ser Glu Asp Asp 
        115                 120                 125             


Leu Lys Val Ala Ile Ile Arg Ala Asn Thr Asp Val Lys Val Asp Gly 
    130                 135                 140                 


Glu Ile Val Tyr Val Ser Ser Gln Asn Val Lys Leu Thr Gly Lys Asp 
145                 150                 155                 160 


Ser Val Ser Ile Arg Asp Gly Tyr Tyr Leu Glu Thr Gly Ser Val Thr 
                165                 170                 175     


Ala Ser Val Asp Val Thr Gly Gln Glu Ser Val Gly Thr Glu Gln Leu 
            180                 185                 190         


Ser Gly Thr Glu Gln Met Glu Met Thr Gly Glu Pro Val Asn Ala Asp 
        195                 200                 205             


Asp Thr Glu Gln Thr Glu Ala Ala Ala Gly Asp Gly Ser Phe Glu Thr 
    210                 215                 220                 


Asp Val Tyr Thr Phe Ile Val Tyr Lys 
225                 230             


<210>  16
<211>  699
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial variant of SG11  (N83S, C147V, C151S) (codon 
       optimized)

<400>  16
atgttggagg gtgaagagtc tgttgtctat gtgggtaaga aaggtgtgat cgcgtccctg       60

gacgtcgaga ctctggacca gtcttactat gatgaaaccg agctgaagtc gtatgtggac      120

gccgaagttg aggattacac ggccgagcac ggcaaaaatg ccgtcaaagt tgagagcttg      180

aaagttgagg acggcgtggc aaagctgaag atgaaataca agaccccaga ggactacacg      240

gcgttcagcg gtatcgagct gtatcagggc aaagtcgtcg catccctggc agcgggctat      300

gtgtacgacg gtgagtttgc gcgcgtcgaa gaaggcaaag ttgtgggtgc ggctacgaaa      360

caagatatct acagcgaaga tgacctgaaa gtcgcgatta ttcgtgctaa caccgatgtt      420

aaagttgatg gcgagattgt gtacgttagc agccaaaacg taaagctgac gggtaaagat      480

agcgtgagca ttcgtgatgg ctattatctg gaaaccggta gcgttacggc gagcgtcgat      540

gttaccggtc aagagagcgt gggtaccgaa cagctgagcg gcaccgaaca gatggaaatg      600

accggtgaac cggttaacgc agacgacacg gaacaaaccg aagccgcggc aggcgacggt      660

agcttcgaga ctgacgtgta cacctttatc gtgtacaag                             699


<210>  17
<211>  233
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial variant of SG11  (N53S, G84D, C147V, C151S)

<400>  17

Met Leu Glu Gly Glu Glu Ser Val Val Tyr Val Gly Lys Lys Gly Val 
1               5                   10                  15      


Ile Ala Ser Leu Asp Val Glu Thr Leu Asp Gln Ser Tyr Tyr Asp Glu 
            20                  25                  30          


Thr Glu Leu Lys Ser Tyr Val Asp Ala Glu Val Glu Asp Tyr Thr Ala 
        35                  40                  45              


Glu His Gly Lys Ser Ala Val Lys Val Glu Ser Leu Lys Val Glu Asp 
    50                  55                  60                  


Gly Val Ala Lys Leu Lys Met Lys Tyr Lys Thr Pro Glu Asp Tyr Thr 
65                  70                  75                  80  


Ala Phe Asn Asp Ile Glu Leu Tyr Gln Gly Lys Val Val Ala Ser Leu 
                85                  90                  95      


Ala Ala Gly Tyr Val Tyr Asp Gly Glu Phe Ala Arg Val Glu Glu Gly 
            100                 105                 110         


Lys Val Val Gly Ala Ala Thr Lys Gln Asp Ile Tyr Ser Glu Asp Asp 
        115                 120                 125             


Leu Lys Val Ala Ile Ile Arg Ala Asn Thr Asp Val Lys Val Asp Gly 
    130                 135                 140                 


Glu Ile Val Tyr Val Ser Ser Gln Asn Val Lys Leu Thr Gly Lys Asp 
145                 150                 155                 160 


Ser Val Ser Ile Arg Asp Gly Tyr Tyr Leu Glu Thr Gly Ser Val Thr 
                165                 170                 175     


Ala Ser Val Asp Val Thr Gly Gln Glu Ser Val Gly Thr Glu Gln Leu 
            180                 185                 190         


Ser Gly Thr Glu Gln Met Glu Met Thr Gly Glu Pro Val Asn Ala Asp 
        195                 200                 205             


Asp Thr Glu Gln Thr Glu Ala Ala Ala Gly Asp Gly Ser Phe Glu Thr 
    210                 215                 220                 


Asp Val Tyr Thr Phe Ile Val Tyr Lys 
225                 230             


<210>  18
<211>  699
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial variant of SG11  (N53S, G84D, C147V, C151S) (codon 
       optimized)

<400>  18
atgttggagg gtgaagagtc tgttgtctat gtgggtaaga aaggtgtgat cgcgtccctg       60

gacgtcgaga ctctggacca gtcttactat gatgaaaccg agctgaagtc gtatgtggac      120

gccgaagttg aggattacac ggccgagcac ggcaaatccg ccgtcaaagt tgagagcttg      180

aaagttgagg acggcgtggc aaagctgaag atgaaataca agaccccaga ggactacacg      240

gcgttcaatg acatcgagct gtatcagggc aaagtcgtcg catccctggc agcgggctat      300

gtgtacgacg gtgagtttgc gcgcgtcgaa gaaggcaaag ttgtgggtgc ggctacgaaa      360

caagatatct acagcgaaga tgacctgaaa gtcgcgatta ttcgtgctaa caccgatgtt      420

aaagttgatg gcgagattgt gtacgttagc agccaaaacg taaagctgac gggtaaagat      480

agcgtgagca ttcgtgatgg ctattatctg gaaaccggta gcgttacggc gagcgtcgat      540

gttaccggtc aagagagcgt gggtaccgaa cagctgagcg gcaccgaaca gatggaaatg      600

accggtgaac cggttaacgc agacgacacg gaacaaaccg aagccgcggc aggcgacggt      660

agcttcgaga ctgacgtgta cacctttatc gtgtacaag                             699


<210>  19
<211>  233
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial variant of SG11  (N53S, N83S, C147V, C151S)

<400>  19

Met Leu Glu Gly Glu Glu Ser Val Val Tyr Val Gly Lys Lys Gly Val 
1               5                   10                  15      


Ile Ala Ser Leu Asp Val Glu Thr Leu Asp Gln Ser Tyr Tyr Asp Glu 
            20                  25                  30          


Thr Glu Leu Lys Ser Tyr Val Asp Ala Glu Val Glu Asp Tyr Thr Ala 
        35                  40                  45              


Glu His Gly Lys Ser Ala Val Lys Val Glu Ser Leu Lys Val Glu Asp 
    50                  55                  60                  


Gly Val Ala Lys Leu Lys Met Lys Tyr Lys Thr Pro Glu Asp Tyr Thr 
65                  70                  75                  80  


Ala Phe Ser Gly Ile Glu Leu Tyr Gln Gly Lys Val Val Ala Ser Leu 
                85                  90                  95      


Ala Ala Gly Tyr Val Tyr Asp Gly Glu Phe Ala Arg Val Glu Glu Gly 
            100                 105                 110         


Lys Val Val Gly Ala Ala Thr Lys Gln Asp Ile Tyr Ser Glu Asp Asp 
        115                 120                 125             


Leu Lys Val Ala Ile Ile Arg Ala Asn Thr Asp Val Lys Val Asp Gly 
    130                 135                 140                 


Glu Ile Val Tyr Val Ser Ser Gln Asn Val Lys Leu Thr Gly Lys Asp 
145                 150                 155                 160 


Ser Val Ser Ile Arg Asp Gly Tyr Tyr Leu Glu Thr Gly Ser Val Thr 
                165                 170                 175     


Ala Ser Val Asp Val Thr Gly Gln Glu Ser Val Gly Thr Glu Gln Leu 
            180                 185                 190         


Ser Gly Thr Glu Gln Met Glu Met Thr Gly Glu Pro Val Asn Ala Asp 
        195                 200                 205             


Asp Thr Glu Gln Thr Glu Ala Ala Ala Gly Asp Gly Ser Phe Glu Thr 
    210                 215                 220                 


Asp Val Tyr Thr Phe Ile Val Tyr Lys 
225                 230             


<210>  20
<211>  699
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial variant of SG11  (N53S, N83S, C147V, C151S) (codon 
       optimized)

<400>  20
atgttggagg gtgaagagtc tgttgtctat gtgggtaaga aaggtgtgat cgcgtccctg       60

gacgtcgaga ctctggacca gtcttactat gatgaaaccg agctgaagtc gtatgtggac      120

gccgaagttg aggattacac ggccgagcac ggcaaatccg ccgtcaaagt tgagagcttg      180

aaagttgagg acggcgtggc aaagctgaag atgaaataca agaccccaga ggactacacg      240

gcgttcagcg gtatcgagct gtatcagggc aaagtcgtcg catccctggc agcgggctat      300

gtgtacgacg gtgagtttgc gcgcgtcgaa gaaggcaaag ttgtgggtgc ggctacgaaa      360

caagatatct acagcgaaga tgacctgaaa gtcgcgatta ttcgtgctaa caccgatgtt      420

aaagttgatg gcgagattgt gtacgttagc agccaaaacg taaagctgac gggtaaagat      480

agcgtgagca ttcgtgatgg ctattatctg gaaaccggta gcgttacggc gagcgtcgat      540

gttaccggtc aagagagcgt gggtaccgaa cagctgagcg gcaccgaaca gatggaaatg      600

accggtgaac cggttaacgc agacgacacg gaacaaaccg aagccgcggc aggcgacggt      660

agcttcgaga ctgacgtgta cacctttatc gtgtacaag                             699


<210>  21
<211>  227
<212>  PRT
<213>  Roseburia intestinalis

<400>  21

Met Leu Asp Ala Asp Thr Asp Thr Val Tyr Val Gln Lys Asn Gly Thr 
1               5                   10                  15      


Val Leu Ser Val Asp Val Glu Thr Leu Asp Lys Asp Tyr Tyr Asp Glu 
            20                  25                  30          


Thr Glu Leu Lys Asp Tyr Val Thr Asp Ala Val Ser Thr Tyr Thr Gly 
        35                  40                  45              


Glu His Gly Lys Ser Ala Val Lys Leu Glu Asn Leu Ser Val Lys Asp 
    50                  55                  60                  


Gly Thr Ala Thr Leu Lys Met Lys Tyr Lys Thr Pro Glu Asp Tyr Thr 
65                  70                  75                  80  


Gly Phe Asn Gly Ile Glu Leu Tyr Glu Gly Lys Val Val Lys Ala Leu 
                85                  90                  95      


Ala Ala Gly Tyr Asp Phe Lys Thr Asp Phe Val Ser Val Glu Asp Gly 
            100                 105                 110         


Lys Val Thr Gly Thr Ala Thr Lys Glu Glu Ile Tyr Ser Gly Glu Asp 
        115                 120                 125             


Leu Lys Val Val Ile Ile Lys Ala Asn Arg Asp Val Lys Val Asp Gly 
    130                 135                 140                 


Thr Ile Cys Tyr Val Ser Ser Glu Asn Val Lys Leu Thr Gly Thr Asp 
145                 150                 155                 160 


Ser Val Ser Ile Arg Asp Gly Tyr Ser Leu Asn Ser Gly Ser Thr Ala 
                165                 170                 175     


Asp Glu Ser Asp Ser Asp Glu Asn Ile Ala Asp Gly Thr Glu Ser Ile 
            180                 185                 190         


Gly Gly Ser Thr Glu Val Ser Asp Thr Asp Val Asn Asp Asp Thr Thr 
        195                 200                 205             


Tyr Val Lys Asp Asp Gly Ala Phe Glu Thr Asp Val Tyr Thr Tyr Ile 
    210                 215                 220                 


Ile Tyr Lys 
225         


<210>  22
<211>  215
<212>  PRT
<213>  Roseburia sp. 831b

<400>  22

Met Leu Asp Val Glu Glu Ser Thr Val Tyr Val Gln Lys Asn Gly Ser 
1               5                   10                  15      


Val Ile Ser Thr Asp Ile Glu Asp Phe Ser Ala Asp Tyr Tyr Asp Glu 
            20                  25                  30          


Asp Glu Leu Lys Asp Tyr Ile Gly Asp Glu Ile Ser Ser Tyr Thr Ser 
        35                  40                  45              


Glu Asn Gly Lys Lys Ser Val Ser Leu Glu Ser Val Ser Val Lys Asp 
    50                  55                  60                  


Ser Val Ala Lys Leu Thr Met Lys Tyr Lys Thr Ala Glu Asp Tyr Thr 
65                  70                  75                  80  


Asn Phe Asn Gly Val Glu Leu Tyr Thr Gly Thr Ile Val Lys Ala Met 
                85                  90                  95      


Ala Ala Gly Tyr Asp Phe Gly Val Asp Phe Val Ser Val Lys Asp Gly 
            100                 105                 110         


Ala Val Thr Gly Thr Ala Thr Lys Asp Glu Ile Val Asp His Asp Asp 
        115                 120                 125             


Tyr Lys Val Ala Val Ile Lys Ala Asn Thr Asp Val Lys Val Asp Gly 
    130                 135                 140                 


Thr Ile Val Tyr Val Ser Ser Gln Asn Val Lys Val Thr Gly Lys Asn 
145                 150                 155                 160 


Thr Val Ser Ile Arg Glu Gly Tyr Leu Ala Ala Asp Thr Thr Asn Val 
                165                 170                 175     


Val Gly Ser Thr Glu Thr Val Ala Glu Thr Glu Ala Glu Glu Ala Asn 
            180                 185                 190         


Gln Thr Glu Ala Val Leu Glu Asp Glu Phe Ala Ser Glu Ser Asp Val 
        195                 200                 205             


Tyr Thr Tyr Val Ile Phe Lys 
    210                 215 


<210>  23
<211>  236
<212>  PRT
<213>  Roseburia inulinivorans

<400>  23

Met Leu Glu Ala Asp Thr Asn Thr Val Tyr Val Ser Lys His Gly Lys 
1               5                   10                  15      


Val Val Ser Met Asp Val Glu Gln Leu Asp Gln Ser Tyr Tyr Asp Glu 
            20                  25                  30          


Thr Glu Leu Lys Glu Phe Val Asp Ser Ala Val Asp Glu Tyr Asn Thr 
        35                  40                  45              


Glu Asn Gly Lys Asn Ser Val Lys Val Asp Asp Leu Thr Val Glu Asp 
    50                  55                  60                  


Gly Thr Ala Lys Leu Arg Met Asp Tyr Glu Thr Val Asp Asp Tyr Thr 
65                  70                  75                  80  


Ala Phe Asn Gly Val Glu Leu Tyr Glu Gly Lys Ile Val Gln Ala Leu 
                85                  90                  95      


Ala Ala Gly Tyr Asp Phe Asp Thr Asp Phe Ala Gly Val Asp Lys Asp 
            100                 105                 110         


Gly Cys Val Thr Gly Val Thr Arg Gly Asp Ile Leu Ala Gln Glu Asp 
        115                 120                 125             


Leu Lys Val Val Ile Ile Lys Ala Asn Thr Asp Val Lys Ile Asp Gly 
    130                 135                 140                 


Lys Ile Leu Tyr Val Ser Cys Asp Asn Val Thr Val Thr Gly Lys Asp 
145                 150                 155                 160 


Ser Val Ser Ile Lys Glu Gly Thr Gly Ile Glu Lys Thr Trp Ile Thr 
                165                 170                 175     


Glu Ala Glu Glu Val Pro Ser Thr Glu Ala Val Leu Glu Thr Glu Ser 
            180                 185                 190         


Thr Glu Asp Ala Gly Asp Val Ile Glu Gly Glu Val Ile Ile Gly Thr 
        195                 200                 205             


Glu Glu Ala Ser Gly Asn Asp Val Val Thr Asn Leu Ser Gly Gly Ser 
    210                 215                 220                 


Ser Gly Thr Asp Val Tyr Thr Tyr Ile Ile Tyr Lys 
225                 230                 235     


<210>  24
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragment of SG11 with start methionine 

<400>  24

Met Leu Glu Gly Glu Glu Ser Val Val Tyr Val Gly Lys 
1               5                   10              


<210>  25
<211>  22
<212>  PRT
<213>  Roseburia hominis

<400>  25

Gly Val Ile Ala Ser Leu Asp Val Glu Thr Leu Asp Gln Ser Tyr Tyr 
1               5                   10                  15      


Asp Glu Thr Glu Leu Lys 
            20          


<210>  26
<211>  17
<212>  PRT
<213>  Roseburia hominis

<400>  26

Thr Pro Glu Asp Tyr Thr Ala Phe Asn Gly Ile Glu Leu Tyr Gln Gly 
1               5                   10                  15      


Lys 
    


<210>  27
<211>  6
<212>  PRT
<213>  Roseburia hominis

<400>  27

Ala Asn Thr Asp Val Lys 
1               5       


<210>  28
<211>  14
<212>  PRT
<213>  Roseburia hominis

<400>  28

Val Asp Gly Glu Ile Cys Tyr Val Ser Cys Gln Asn Val Lys 
1               5                   10                  


<210>  29
<211>  67
<212>  PRT
<213>  Roseburia hominis

<400>  29

Gly Tyr Tyr Leu Glu Thr Gly Ser Val Thr Ala Ser Val Asp Val Thr 
1               5                   10                  15      


Gly Gln Glu Ser Val Gly Thr Glu Gln Leu Ser Gly Thr Glu Gln Met 
            20                  25                  30          


Glu Met Thr Gly Glu Pro Val Asn Ala Asp Asp Thr Glu Gln Thr Glu 
        35                  40                  45              


Ala Ala Ala Gly Asp Gly Ser Phe Glu Thr Asp Val Tyr Thr Phe Ile 
    50                  55                  60                  


Val Tyr Lys 
65          


<210>  30
<211>  4
<212>  PRT
<213>  Roseburia hominis

<400>  30

Asn Ala Val Lys 
1               


<210>  31
<211>  17
<212>  PRT
<213>  Roseburia hominis

<400>  31

Thr Pro Glu Asp Tyr Thr Ala Phe Ser Gly Ile Glu Leu Tyr Gln Gly 
1               5                   10                  15      


Lys 
    


<210>  32
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FLAG tag

<400>  32

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  33
<211>  233
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial variant of SG11


<220>
<221>  misc_feature
<222>  (53)..(53)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (83)..(84)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (147)..(147)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (151)..(151)
<223>  Xaa can be any naturally occurring amino acid

<400>  33

Met Leu Glu Gly Glu Glu Ser Val Val Tyr Val Gly Lys Lys Gly Val 
1               5                   10                  15      


Ile Ala Ser Leu Asp Val Glu Thr Leu Asp Gln Ser Tyr Tyr Asp Glu 
            20                  25                  30          


Thr Glu Leu Lys Ser Tyr Val Asp Ala Glu Val Glu Asp Tyr Thr Ala 
        35                  40                  45              


Glu His Gly Lys Xaa Ala Val Lys Val Glu Ser Leu Lys Val Glu Asp 
    50                  55                  60                  


Gly Val Ala Lys Leu Lys Met Lys Tyr Lys Thr Pro Glu Asp Tyr Thr 
65                  70                  75                  80  


Ala Phe Xaa Xaa Ile Glu Leu Tyr Gln Gly Lys Val Val Ala Ser Leu 
                85                  90                  95      


Ala Ala Gly Tyr Val Tyr Asp Gly Glu Phe Ala Arg Val Glu Glu Gly 
            100                 105                 110         


Lys Val Val Gly Ala Ala Thr Lys Gln Asp Ile Tyr Ser Glu Asp Asp 
        115                 120                 125             


Leu Lys Val Ala Ile Ile Arg Ala Asn Thr Asp Val Lys Val Asp Gly 
    130                 135                 140                 


Glu Ile Xaa Tyr Val Ser Xaa Gln Asn Val Lys Leu Thr Gly Lys Asp 
145                 150                 155                 160 


Ser Val Ser Ile Arg Asp Gly Tyr Tyr Leu Glu Thr Gly Ser Val Thr 
                165                 170                 175     


Ala Ser Val Asp Val Thr Gly Gln Glu Ser Val Gly Thr Glu Gln Leu 
            180                 185                 190         


Ser Gly Thr Glu Gln Met Glu Met Thr Gly Glu Pro Val Asn Ala Asp 
        195                 200                 205             


Asp Thr Glu Gln Thr Glu Ala Ala Ala Gly Asp Gly Ser Phe Glu Thr 
    210                 215                 220                 


Asp Val Tyr Thr Phe Ile Val Tyr Lys 
225                 230             


<210>  34
<211>  233
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial variant of SG11


<220>
<221>  misc_feature
<222>  (53)..(53)
<223>  Xaa is any amino acid other than Asn

<220>
<221>  misc_feature
<222>  (83)..(83)
<223>  Xaa is any amino acid other than Asn

<220>
<221>  misc_feature
<222>  (84)..(84)
<223>  Xaa is any amino acid other than Gly

<220>
<221>  misc_feature
<222>  (147)..(147)
<223>  Xaa is any amino acid other than Cys

<220>
<221>  misc_feature
<222>  (151)..(151)
<223>  Xaa is any amino acid other than Cys

<400>  34

Met Leu Glu Gly Glu Glu Ser Val Val Tyr Val Gly Lys Lys Gly Val 
1               5                   10                  15      


Ile Ala Ser Leu Asp Val Glu Thr Leu Asp Gln Ser Tyr Tyr Asp Glu 
            20                  25                  30          


Thr Glu Leu Lys Ser Tyr Val Asp Ala Glu Val Glu Asp Tyr Thr Ala 
        35                  40                  45              


Glu His Gly Lys Xaa Ala Val Lys Val Glu Ser Leu Lys Val Glu Asp 
    50                  55                  60                  


Gly Val Ala Lys Leu Lys Met Lys Tyr Lys Thr Pro Glu Asp Tyr Thr 
65                  70                  75                  80  


Ala Phe Xaa Xaa Ile Glu Leu Tyr Gln Gly Lys Val Val Ala Ser Leu 
                85                  90                  95      


Ala Ala Gly Tyr Val Tyr Asp Gly Glu Phe Ala Arg Val Glu Glu Gly 
            100                 105                 110         


Lys Val Val Gly Ala Ala Thr Lys Gln Asp Ile Tyr Ser Glu Asp Asp 
        115                 120                 125             


Leu Lys Val Ala Ile Ile Arg Ala Asn Thr Asp Val Lys Val Asp Gly 
    130                 135                 140                 


Glu Ile Xaa Tyr Val Ser Xaa Gln Asn Val Lys Leu Thr Gly Lys Asp 
145                 150                 155                 160 


Ser Val Ser Ile Arg Asp Gly Tyr Tyr Leu Glu Thr Gly Ser Val Thr 
                165                 170                 175     


Ala Ser Val Asp Val Thr Gly Gln Glu Ser Val Gly Thr Glu Gln Leu 
            180                 185                 190         


Ser Gly Thr Glu Gln Met Glu Met Thr Gly Glu Pro Val Asn Ala Asp 
        195                 200                 205             


Asp Thr Glu Gln Thr Glu Ala Ala Ala Gly Asp Gly Ser Phe Glu Thr 
    210                 215                 220                 


Asp Val Tyr Thr Phe Ile Val Tyr Lys 
225                 230             


