                               SEQUENCE LISTING

<110> AMIDEBIO, LLC
 
<120> GLUCOSE-RESPONSIVE INSULIN ANALOGS AND METHODS OF USE THEREOF

<130> 39538-712.601

<140> PCT/US2018/025070
<141> 2018-03-29

<150> 62/480,859
<151> 2017-04-03

<160> 32    

<170> PatentIn version 3.5

<210> 1
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (5)..(5)
<223> Any amino acid

<400> 1
Tyr Pro Gly Asp Xaa 
1               5   


<210> 2
<211> 58
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 2
Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Arg Arg 
            20                  25                  30          


Tyr Pro Gly Asp Val Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys 
        35                  40                  45              


Ser Leu Tyr Gln Leu Glu Asn Tyr Cys Gly 
    50                  55              


<210> 3
<211> 56
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 3
Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Arg Val 
            20                  25                  30          


Asp Gly Val Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu 
        35                  40                  45              


Tyr Gln Leu Glu Asn Tyr Cys Gly 
    50                  55      


<210> 4
<211> 55
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 4
Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Val Asp 
            20                  25                  30          


Gly Lys Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr 
        35                  40                  45              


Gln Leu Glu Asn Tyr Cys Gly 
    50                  55  


<210> 5
<211> 55
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 5
Phe Val Asp Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Tyr Asp 
            20                  25                  30          


Gly Lys Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr 
        35                  40                  45              


Gln Leu Glu Asn Tyr Cys Gly 
    50                  55  


<210> 6
<211> 55
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 6
Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Tyr Asp 
            20                  25                  30          


Gly Lys Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr 
        35                  40                  45              


Gln Leu Glu Asn Tyr Cys Gly 
    50                  55  


<210> 7
<211> 55
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 7
Phe Val Gly Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Tyr Asp 
            20                  25                  30          


Gly Lys Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr 
        35                  40                  45              


Gln Leu Glu Asn Tyr Cys Gly 
    50                  55  


<210> 8
<211> 21
<212> PRT
<213> Homo sapiens

<400> 8
Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Asn 
            20      


<210> 9
<211> 30
<212> PRT
<213> Homo sapiens

<400> 9
Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr 
            20                  25                  30  


<210> 10
<211> 58
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide


<220>
<221> MOD_RES
<222> (1)..(1)
<223> F or absent 

<220>
<221> MOD_RES
<222> (2)..(2)
<223> V or absent 

<220>
<221> MOD_RES
<222> (3)..(3)
<223> N, A, K, D, or absent 

<220>
<221> MOD_RES
<222> (31)..(31)
<223> R or absent 

<220>
<221> MOD_RES
<222> (32)..(32)
<223> R or absent 

<220>
<221> MOD_RES
<222> (33)..(33)
<223> Y or V 

<220>
<221> MOD_RES
<222> (34)..(34)
<223> P or absent 

<220>
<221> MOD_RES
<222> (35)..(35)
<223> G or D 

<220>
<221> MOD_RES
<222> (36)..(36)
<223> D or G 

<220>
<221> MOD_RES
<222> (37)..(37)
<223> V or K 

<220>
<221> MOD_RES
<222> (58)..(58)
<223> N, G, A, D, or Q 

<400> 10
Xaa Xaa Xaa Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Xaa Xaa 
            20                  25                  30          


Xaa Xaa Xaa Xaa Xaa Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys 
        35                  40                  45              


Ser Leu Tyr Gln Leu Glu Asn Tyr Cys Xaa 
    50                  55              


<210> 11
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (1)..(1)
<223> R or absent 

<220>
<221> MOD_RES
<222> (2)..(2)
<223> R or absent 

<220>
<221> MOD_RES
<222> (3)..(3)
<223> Y or V 

<220>
<221> MOD_RES
<222> (4)..(4)
<223> P or absent 

<220>
<221> MOD_RES
<222> (5)..(5)
<223> G or D 

<220>
<221> MOD_RES
<222> (6)..(6)
<223> D or G 

<220>
<221> MOD_RES
<222> (7)..(7)
<223> V or K 

<400> 11
Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
1               5           


<210> 12
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (1)..(1)
<223> R or absent 

<220>
<221> MOD_RES
<222> (2)..(2)
<223> R or absent 

<220>
<221> MOD_RES
<222> (3)..(3)
<223> Y or V 

<220>
<221> MOD_RES
<222> (4)..(4)
<223> P or absent 

<220>
<221> MOD_RES
<222> (7)..(7)
<223> V or K 

<400> 12
Xaa Xaa Xaa Xaa Gly Asp Xaa 
1               5           


<210> 13
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (1)..(1)
<223> R or absent 

<220>
<221> MOD_RES
<222> (2)..(2)
<223> R or absent 

<220>
<221> MOD_RES
<222> (3)..(3)
<223> Y or V 

<220>
<221> MOD_RES
<222> (4)..(4)
<223> P or absent 

<220>
<221> MOD_RES
<222> (7)..(7)
<223> V or K 

<400> 13
Xaa Xaa Xaa Xaa Asp Gly Xaa 
1               5           


<210> 14
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (1)..(1)
<223> R or absent 

<220>
<221> MOD_RES
<222> (2)..(2)
<223> R or absent 

<220>
<221> MOD_RES
<222> (3)..(3)
<223> Y or V 

<220>
<221> MOD_RES
<222> (4)..(4)
<223> P or absent 

<400> 14
Xaa Xaa Xaa Xaa Asp Gly Val 
1               5           


<210> 15
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (1)..(1)
<223> R or absent 

<220>
<221> MOD_RES
<222> (2)..(2)
<223> R or absent 

<220>
<221> MOD_RES
<222> (3)..(3)
<223> Y or V 

<220>
<221> MOD_RES
<222> (4)..(4)
<223> P or absent 

<400> 15
Xaa Xaa Xaa Xaa Asp Gly Lys 
1               5           


<210> 16
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 16
Arg Arg Tyr Pro Gly Asp Val 
1               5           


<210> 17
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 17
Arg Arg Tyr Pro Asp Gly Val 
1               5           


<210> 18
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 18
Arg Arg Val Asp Gly Val 
1               5       


<210> 19
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 19
Arg Tyr Pro Asp Gly Val 
1               5       


<210> 20
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 20
Arg Val Asp Gly Val 
1               5   


<210> 21
<211> 4
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 21
Val Asp Gly Lys 
1               


<210> 22
<211> 4
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 22
Tyr Asp Gly Lys 
1               


<210> 23
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MISC_FEATURE
<222> (1)..(10) 
<223> This sequence may encompass 5-10 residues

<400> 23
Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys 
1               5                   10  


<210> 24
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MISC_FEATURE
<222> (1)..(10) 
<223> This sequence may encompass 5-10 residues

<400> 24
His His His His His His His His His His 
1               5                   10  


<210> 25
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MISC_FEATURE
<222> (1)..(10) 
<223> This sequence may encompass 5-10 residues

<400> 25
Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu 
1               5                   10  


<210> 26
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MISC_FEATURE
<222> (1)..(10) 
<223> This sequence may encompass 5-10 residues

<400> 26
Arg Arg Arg Arg Arg Arg Arg Arg Arg Arg 
1               5                   10  


<210> 27
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 27
His His His His His His 
1               5       


<210> 28
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 28
Lys Lys Lys Lys Lys Lys 
1               5       


<210> 29
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 29
Glu Glu Glu Glu Glu Glu 
1               5       


<210> 30
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 30
Arg Arg Arg Arg Arg Arg 
1               5       


<210> 31
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (1)..(1)
<223> R or absent 

<220>
<221> MOD_RES
<222> (2)..(2)
<223> R or absent 

<220>
<221> MOD_RES
<222> (3)..(3)
<223> Y or V 

<220>
<221> MOD_RES
<222> (4)..(4)
<223> P or absent 

<220>
<221> MOD_RES
<222> (5)..(5)
<223> G or D 

<220>
<221> MOD_RES
<222> (6)..(6)
<223> D or G 

<220>
<221> MOD_RES
<222> (7)..(7)
<223> Any amino acid

<400> 31
Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
1               5           


<210> 32
<211> 58
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide


<220>
<221> MOD_RES
<222> (1)..(1)
<223> F or absent 

<220>
<221> MOD_RES
<222> (2)..(2)
<223> V or absent 

<220>
<221> MOD_RES
<222> (3)..(3)
<223> N, A, K, D, or absent 

<220>
<221> MOD_RES
<222> (31)..(31)
<223> R or absent 

<220>
<221> MOD_RES
<222> (32)..(32)
<223> R or absent 

<220>
<221> MOD_RES
<222> (33)..(33)
<223> Y, R, or absent 

<220>
<221> MOD_RES
<222> (34)..(34)
<223> P, V or Y 

<220>
<221> MOD_RES
<222> (35)..(35)
<223> G or D 

<220>
<221> MOD_RES
<222> (36)..(36)
<223> D or G 

<220>
<221> MOD_RES
<222> (37)..(37)
<223> V or K 

<220>
<221> MOD_RES
<222> (58)..(58)
<223> N, G, A, D, or Q 

<400> 32
Xaa Xaa Xaa Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Xaa Xaa 
            20                  25                  30          


Xaa Xaa Xaa Xaa Xaa Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys 
        35                  40                  45              


Ser Leu Tyr Gln Leu Glu Asn Tyr Cys Xaa 
    50                  55              



