                         SEQUENCE LISTING

<110>  LALI, ARVIND MALLINATH
 
<120>  METHODS FOR OBTAINING RECOMBINANT SYNECHOCOCCUS FOR ENHANCED 
       BIO-PRODUCTION OF ZEAXANTHIN

<130>  PD028669IN-SC

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  996
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crtR1 gene of Synechococcus elongatus PCC7942

<400>  1
atgtcagagg ctcaaacgcc cctgacagta ccgaagaagt ttcttggtgc tccaggaggc       60

ttcaacccca ccgtcgcact cttcttggca ggttatacct gcgcggcgct ctcagttttg      120

gggtactggt gctggagttg gccccactgg ctatctttcc ttctgagtgt cacagcctta      180

catttggtag gcaccgtcat tcacgatgcc tctcataatg tggctcacgc cagtcgcatt      240

ctgaatgcga ttttgggaca tggcagtgca ctattgctgg gctttacttt tccggtgttt      300

acgcgggttc acctgcaaca tcacgcccac gtcaacgatc ccaagaacga tcccgaccac      360

atcgtttcca cctttgggcc gctgtggttg atcgcaccgc gcttcttcta tcacgagatc      420

tatttcttcc agcgccgcct ttggaagaaa tttgaattac tcgaatggtt cctcagtcgc      480

gctgtggtca tcggcatctt tgcctgcggc gtcaagtttg gcttcctggg cttcctgatg      540

aactactggc tggctccagc cttggtcgtt ggcattgccc taggactctt cttcgactat      600

ttaccccacc gccccttcca agagcgcaac cgctggcgca atgcacgggt ctatcccggt      660

caggtgatga acatcctgat catgggtcag aactatcacc tgatccatca cctctggcca      720

tcgatcccct ggtatctcta ccgaccggcc taccacgcta ccaagccgtt gttggaccta      780

cgccagtcgc cgcaaacgct cgggattctc tccagcaaaa aagatttctg gaactttatc      840

tacgacgttt tcatcggcat ccgcattcac caatcgcacg aggctgagcc gcagagctcc      900

gtcgttcctg aaacgaagtc gagtgaatca gccgttctcg caaaagctcc gatgtctgcc      960

acagaagact ctcgtgagcc agccttgacg aagtag                                996


<210>  2
<211>  888
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crtR2 gene of Synechococcus elongatus PCC7002

<400>  2
atgacggcgg cggcagcgtc atcattggta atgtcaaggg agtatttgcg tccccctggt       60

gggatgaacc ctaatgtgtg gatggtcatc atcgcagtag gattgatcgc cacctccgtg      120

ggaggctatt ggttttgggg ttggtatgac tggatttgct tcctagaaaa cgttttagcg      180

ctgcaccttg cgggaacggt gatccacgat gcgtcccacc gtgccgccca tagcaaccgt      240

gcggtcaata ccatcttggg ccatgccagt gccctaatgc tgggatttgc tttcccggtt      300

tttacccggg tccatctcca gcaccacgcc catgtcaatg atcctgaaaa tgatccggat      360

catttcgttt cgactggcgg ccccctctgg atgattgcag cccgtttttt ctaccacgaa      420

atctttttct ttaagcgtcg tctgtggaaa aattacgaat tactggagtg gttccttagc      480

cgtgcttttt taggagtcat tgtctaccta ggcatccagt atggcttcat tggctacatc      540

atgaactttt ggtttgttcc ggccctggtg gttggtattg ccctgggttt gttctttgat      600

tatttacccc atcgtccctt tgaagaacgc gatcgctgga aaaatgccag ggtttaccca      660

agcaagctcc tcaatctttt gatccttggg caaaattacc acctagtcca tcacctgtgg      720

ccctcaattc cttggtataa gtatcaaccc gcctattact acatcaagcc cctgttagat      780

caaaaaggtt ctccccaatc attagggctg ctccagggaa aagattttct cagtttcctc      840

tacgatattt ttgtgggcat tcgcctgcac cacaaaccca aatcctaa                   888


<210>  3
<211>  1395
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  galP gene sequence

<400>  3
atgcctgacg ctaaaaaaca ggggcggtca aacaaggcaa tgacgttttt cgtctgcttc       60

cttgccgctc tggcgggatt actctttggc ctggatatcg gtgtaattgc tggcgcactg      120

ccgtttattg cagatgaatt ccagattact tcgcacacgc aagaatgggt cgtaagctcc      180

atgatgttcg gtgcggcagt cggtgcggtg ggcagcggct ggctctcctt taaactcggg      240

cgcaaaaaga gcctgatgat cggcgcaatt ttgtttgttg ccggttcgct gttctctgcg      300

gctgcgccaa acgttgaagt actgattctt tcccgcgttc tactggggct ggcggtgggt      360

gtggcctctt ataccgcacc gctgtacctc tctgaaattg cgccggaaaa aattcgtggc      420

agtatgatct cgatgtatca gttgatgatc actatcggga tcctcggtgc ttatctttct      480

gataccgcct tcagctacac cggtgcatgg cgctggatgc tgggtgtgat tatcatcccg      540

gcaattttgc tgctgattgg tgtcttcttc ctgccagaca gcccacgttg gtttgccgcc      600

aaacgccgtt ttgttgatgc cgaacgcgtg ctgctacgcc tgcgtgacac cagcgcggaa      660

gcgaaacgcg aactggatga aatccgtgaa agtttgcagg ttaaacagag tggctgggcg      720

ctgtttaaag agaacagcaa cttccgccgc gcggtgttcc ttggcgtact gttgcaggta      780

atgcagcaat tcaccgggat gaacgtcatc atgtattacg cgccgaaaat cttcgaactg      840

gcgggttata ccaacactac cgagcaaatg tgggggaccg tgattgtcgg cctgaccaac      900

gtacttgcca cctttatcgc aatcggcctt gttgaccgct ggggacgtaa accaacgcta      960

acgctgggct tcctggtgat ggctgctggc atgggcgtac tcggtacaat gatgcatatc     1020

ggtattcact ctccgtcggc gcagtatttc gccatcgcca tgctgctgat gtttattgtc     1080

ggttttgcca tgagtgccgg tccgctgatt tgggtactgt gctccgaaat tcagccgctg     1140

aaaggccgcg attttggcat cacctgctcc actgccacca actggattgc caacatgatc     1200

gttggcgcaa cgttcctgac catgctcaac acgctgggta acgccaacac cttctgggtg     1260

tatgcggctc tgaacgtact gtttatcctg ctgacattgt ggctggtacc ggaaaccaaa     1320

cacgtttcgc tggaacatat tgaacgtaat ctgatgaaag gtcgtaaact gcgcgaaata     1380

ggcgctcacg attaa                                                      1395


<210>  4
<211>  996
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crtR1 gene of Synechococcus elongatus PCC 6301

<400>  4
atgtcagagg ctcaaacgcc cctgacagta ccgaagaagt ttcttggtgc tccaggaggc       60

ttcaacccca ccgtcgcact cttcttggca ggttatacct gcgcggcgct ctcagttttg      120

gggtactggt gctggagttg gccccactgg ctatctttcc ttctgagtgt cacagcctta      180

catttggtag gcaccgtcat tcacgatgcc tctcataatg tggctcacgc cagtcgcatt      240

ctgaatgcga ttttgggaca tggcagtgca ctattgctgg gctttacttt tccggtgttt      300

acgcgggttc acctgcaaca tcacgcccac gtcaacgatc ccaagaacga tcccgaccac      360

atcgtttcca cctttgggcc gctgtggttg atcgcaccgc gcttcttcta tcacgagatc      420

tatttcttcc agcgccgcct ttggaagaaa tttgaattac tcgaatggtt cctcagtcgc      480

gctgtggtca tcggcatctt tgcctgcggc gtcaagtttg gcttcctggg cttcctgatg      540

aactactggc tggctccagc cttggtcgtt ggcattgccc taggactctt cttcgactat      600

ttaccccacc gccccttcca agagcgcaac cgctggcgca atgcacgggt ctatcccggt      660

caggtgatga acatcctgat catgggtcag aactatcacc tgatccatca cctctggcca      720

tcgatcccct ggtatctcta ccgaccggcc taccacgcta ccaagccgtt gttggaccta      780

cgccagtcgc cgcaaacgct cgggattctc tccagcaaaa aagatttctg gaactttatc      840

tacgacgttt tcatcggcat ccgcattcac caatcgcacg aggctgagcc gcagagctcc      900

gtcgttcctg aaacgaagtc gagtgaatca gccgttctcg caaaagctcc gatgtctgcc      960

acagaagact ctcgtgagcc agccttgacg aagtag                                996


<210>  5
<211>  996
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crtR1 gene of Synechococcus elongatus UTEX 2973

<400>  5
atgtcagagg ctcaaacgcc cctgacagta ccgaagaagt ttcttggtgc tccaggaggc       60

ttcaacccca ccgtcgcact cttcttggca ggttatacct gcgcggcgct ctcagttttg      120

gggtactggt gctggagttg gccccactgg ctatctttcc ttctgagtgt cacagcctta      180

catttggtag gcaccgtcat tcacgatgcc tctcataatg tggctcacgc cagtcgcatt      240

ctgaatgcga ttttgggaca tggcagtgca ctattgctgg gctttacttt tccggtgttt      300

acgcgggttc acctgcaaca tcacgcccac gtcaacgatc ccaagaacga tcccgaccac      360

atcgtttcca cctttgggcc gctgtggttg atcgcaccgc gcttcttcta tcacgagatc      420

tatttcttcc agcgccgcct ttggaagaaa tttgaattac tcgaatggtt cctcagtcgc      480

gctgtggtca tcggcatctt tgcctgcggc gtcaagtttg gcttcctggg cttcctgatg      540

aactactggc tggctccagc cttggtcgtt ggcattgccc taggactctt cttcgactat      600

ttaccccacc gccccttcca agagcgcaac cgctggcgca atgcacgggt ctatcccggt      660

caggtgatga acatcctgat catgggtcag aactatcacc tgatccatca cctctggcca      720

tcgatcccct ggtatctcta ccgaccggcc taccacgcta ccaagccgtt gttggaccta      780

cgccagtcgc cgcaaacgct cgggattctc tccagcaaaa aagatttctg gaactttatc      840

tacgacgttt tcatcggcat ccgcattcac caatcgcacg aggctgagcc gcagagctcc      900

gtcgttcctg aaacgaagtc gagtgaatca gccgttctcg caaaagctcc gatgtctgcc      960

acagaagact ctcgtgagcc agccttgacg aagtag                                996


<210>  6
<211>  1245
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Neutral site I sequence in Synechococcus elongatus PCC 7942

<400>  6
atgtttgaaa cgatttttgc gctgctgatt gttctaggcg ctggcgccgg ggctggcagc       60

ttagtcctgc gcaatctcta ctacatctgc caacccagtg aaattttgat ctttgctggc      120

agtagtcgcc gcagtagtga tggccgccga gttggctatc gcttggtcaa gggcggcagc      180

agcctgcggg tacctctgct ggaaaaagcg ctccgcatgg atctgaccaa catgatcatt      240

gagttgcgcg tttccaatgc cttctccaag ggcggcattc ccctgactgt tgaaggcgtt      300

gccaatatca agattgctgg ggaagaaccg accatccaca acgcgatcga gcggctgctt      360

ggcaaaaacc gtaaggaaat cgagcaaatt gccaaggaga ccctcgaagg caacttgcgt      420

ggtgttttag ccagcctcac gccggagcag atcaacgagg acaaaattgc ctttgccaaa      480

agtctgctgg aagaggcgga ggatgacctt gagcagctgg gtctagtcct cgatacgctg      540

caagtccaga acatttccga tgaggtcggt tatctctcgg ctagtggacg caagcagcgg      600

gctgatctgc agcgagatgc ccgaattgct gaagccgatg cccaggctgc ctctgcgatc      660

caaacggccg aaaatgacaa gatcacggcc ctgcgtcgga tcgatcgcga tgtagcgatc      720

gcccaagccg aggccgagcg ccggattcag gatgcgttga cgcggcgcga agcggtggtg      780

gccgaagctg aagcggacat tgctaccgaa gtcgctcgta gccaagcaga actccctgtg      840

cagcaggagc ggatcaaaca ggtgcagcag caacttcaag ccgatgtgat cgccccagct      900

gaggcagctt gtaaacgggc gatcgcggaa gcgcgggggg ccgccgcccg tatcgtcgaa      960

gatggaaaag ctcaagcgga agggacccaa cggctggcgg aggcttggca gaccgctggt     1020

gctaatgccc gcgacatctt cctgctccag aagctcgagt ccctgctcgt cacgctttca     1080

ggcaccgtgc cagatatcga cgtggagtcg atcactgtga ttggcgaagg ggaaggcagc     1140

gctacccaaa tcgctagctt gctggagaag ctgaaacaaa ccacgggcat tgatctggcg     1200

aaatccctac cgggtcaatc cgactcgccc gctgcgaagt cctaa                     1245


<210>  7
<211>  3115
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Neutral site II sequence in Synechococcus elongatus PCC7942

<400>  7
gatccgccct tgctttgggc gattgattcc gatccggttt tggccggtac gaagctcatt       60

gctgaagctt gggacgcagc cggcttatat caggttggta cctttattgg cgatcgcttt      120

gggacttgga acggtccctt ccgggacgat attcggcgtt tttggcgtgg agatcagggc      180

tgtacttacg ccctcagtca acgcctgctg ggtagccccg atgtctacag cacagaccaa      240

tggtatgccg gacgcaccat taacttcatc acctgccatg acggctttac gctgcgagat      300

ctagtcagct atagccagaa gcacaacttt gccaatggag agaacaatcg ggacgggacc      360

aatgacaact acagctggaa ctacggcatt gaaggcgaga ccgatgaccc cacgattctg      420

agcttacggg aacggcagca gcgcaatttg ctcgccacgt tattcctcgc ccagggcaca      480

ccgatgctga cgatgggcga tgaggtcaaa cgcagtcagc agggtaacaa taacgcctac      540

tgccaagaca atgagatcag ctggtttgat tggtcgctgt gcgatcgcca tgccgatttc      600

ttggtgttca gtcgccgcct gattgaactt tcccagtcgc tggtgatgtt ccaacagaac      660

gaactgctgc agaacgaacc ccatccgcgt cgtccctatg ccatctggca tggcgtcaaa      720

ctcaaacaac ccgattgggc gctgtggtcc cacagtctgg ccgtcagtct ctgccatcct      780

cgccagcagg aatggcttta cctagccttt aatgcttact gggaagacct gcgcttccag      840

ttgccgaggc ctcctcgcgg ccgcgtttgg tatcgcttgc tcgatacttc actgccgaat      900

cttgaagctt gtcatctgcc ggatgaggca aaaccctgcc tacggcgcga ttacatcgtc      960

ccagcgcgat cgctcttact gttgatggct cgtgcttaaa aacaatgcaa acttcaccgt     1020

ttcagctggt gattttcgac tgtgatggtg tgcttgttga tagcggaacg catcactaat     1080

cgcgtctttg cagacatgct caatgaactg ggtctgttgg tgactttgga tgacatgttt     1140

gagcagtttg tgggtcattc catggctgac tgtctcaaac taattgagcg acggttaggc     1200

aatcctccac cccctgactt tgttcagcac tatcaacgcc gtacccgtat cgcgttagaa     1260

acgcatctac aagccgttcc tggggttgaa gaggctttgg atgctcttga attgccctac     1320

tgtgttgcgt ccagtggtga tcatcaaaag atgcgaacca cactgagcct gacgaagctc     1380

tggccacgat ttgagggacg aatcttcagc gtgactgaag tacctcgcgg caagccattt     1440

cccgatgtct ttttgttggc cgccgatcgc ttcggggtta atcctacggc ctgcgctgtg     1500

atcgaagaca cccccttggg agtagcggca ggcgtggcgg caggaatgca agtgtttggc     1560

tacgcgggtt ccatgcccgc ttggcgtctg caagaagccg gtgcccatct catttttgac     1620

gatatgcgac tgctgcccag tctgctccaa tcgtcgccaa aagataactc cacagcattg     1680

cccaatccct aacccctgct cgcgccgcaa ctacacacta aaccgttcct gcgcgatcgc     1740

tcttactgtt gatggctcgt gcttaaaaac aatgcaaccc taaccgtttc agctggtgat     1800

tttcggacga tttggcttac agggataact gagagtcaac agcctctgtc cgtcattgca     1860

cacccatcca tgcactgggg acttgactca tgctgaatca catttccctt gtccattggg     1920

cgagagggga ggggaatctt ctggactctt cactaagcgg cgatcgcagg ttcttctacc     1980

caagcagtgg cgatcgcttg attgcagtct tcaatgctgg cctctgcagc catcgccgcc     2040

accaaagcat cgtaggcggg acgttgttgc tccagtaaag tcttcgcccg taacaatccc     2100

cagcgactgc gtaaatccgc ttcggcagga ttgcgatcga gttgccgcca cagttgtttc     2160

cactgggcgc gatcgtcagc tcccccttcc acgttgccgt agaccagttg ctctgccgct     2220

gcaccggcca tcaacacctg acaccactgt tccagcgatc gctgactgag ttgcccctgt     2280

gcggcttcgg cttctagcgc agctgcttgg aactgcacac ccccgcgacc aggttgtcct     2340

tggcgcagcg cttcccacgc tgagagggtg tagcccgtca cgggtaaccc cagcgcggtt     2400

gctaccaagt agtgacccgc ttcgtgatgc aaaatccgct gacgatattc gggcgatcgc     2460

tgctgaatgc catcgagcag taacgtggca ccccgcccct gccaagtcac cgcatccaga     2520

ctgaacagca ccaagaggct aaaacccaat cccgccggta gcagcggaga actacccagc     2580

attggtccca ccaaagctaa tgccgtcgtg gtaaaaatcg cgatcgccgt cagactcaag     2640

cccagttcgc tcatgcttcc tcatctaggt cacagtcttc ggcgatcgca tcgatctgat     2700

gctgcagcaa gcgttttcca taccggcgat cgcgccgtcg ccctttcgct gccgtggccc     2760

gcttacgagc tcgtttatcg accacgatcg catccaaatc cgcgatcgct tcccagtccg     2820

gcaattcagt ctggggcgtc cgtttcatta atcctgatca ggcacgaaat tgctgtgcgt     2880

agtatcgcgc atagcggcca gcctctgcca acagcgcatc gtgattgcct gcctcaacaa     2940

tctggccgcg ctccatcacc aagatgcggc tggcattacg aaccgtagcc agacggtgag     3000

caatgataaa gaccgtccgt ccctgcatca cccgttctag ggcctcttgc accaaggttt     3060

cggactcgga atcaagcgcc gaagtcgcct catccagaat taaaatgcgt ggatc          3115


<210>  8
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CrtRE_F primer sequence

<400>  8
agttgaattc atgacggcgg cgg                                               23


<210>  9
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CrtRBMX_R primer sequence

<400>  9
atcggatccc aattgctcga gttaggattt gggtttg                                37


<210>  10
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GalPM_F primer sequence

<400>  10
ctaacaattg atgcctgacg ctaaaaaaca ggggcg                                 36


<210>  11
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GalPBg_R primer sequence

<400>  11
ggatagatct ttaatcgtga gcgcctattt cg                                     32


<210>  12
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP48_F primer sequence

<400>  12
gtctttcgac tgagcctttc g                                                 21


<210>  13
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP48_R primer sequence

<400>  13
caggcagcca tcggaagc                                                     18


