               SEQUENCE LISTING

<110> TARGEDYS
      INSTITUT NATIONAL DE LA SANT ET DE LA RECHERCHE MDICALE (INSERM)
      UNIVERSIT DE ROUEN           
      FETISSOV Serguei
      LAMBERT Grgory
      LEGRAND Romain
      LUCAS Nicolas

<120> PROTEINS DERIVED FROM CLPB AND USES THEREOF

<130> CDM - 754/PCT

<160> 22

<170> BiSSAP 1.3.6

<210> 1
<211> 857
<212> PRT
<213> Hafnia alvei


<220> 
<223> ClpB

<400> 1
Met Arg Leu Asp Arg Leu Thr Ser Lys Phe Gln Leu Ala Leu Ala Asp 
1               5                   10                  15      
Ala Gln Ser Leu Ala Leu Gly Arg Asp Asn Gln Phe Ile Glu Pro Val 
            20                  25                  30          
His Leu Met Ser Ala Leu Leu Asn Gln Asp Gly Gly Thr Val Arg Pro 
        35                  40                  45              
Leu Leu Thr Ser Ala Gly Val Asn Thr Ala Ser Leu Arg Gln Glu Leu 
    50                  55                  60                  
Glu Gln Ala Ile Ser Arg Leu Pro Gln Val Glu Gly Ala Gly Gly Asp 
65                  70                  75                  80  
Val Gln Pro Ser Asn Glu Leu Ile Arg Val Leu Asn Leu Cys Asp Lys 
                85                  90                  95      
Leu Ala Gln Lys Arg Asn Asp Thr Phe Ile Ser Ser Glu Leu Phe Val 
            100                 105                 110         
Leu Ala Val Leu Glu Asp Arg Gly Asn Leu Gly Asp Ile Leu Lys Ala 
        115                 120                 125             
Ala Gly Ala Asn Val Gln Lys Val Ser Thr Ala Ile Glu Gln Met Arg 
    130                 135                 140                 
Gly Gly Glu Lys Val Asp Asp Ala Asn Ala Glu Asp Gln Arg Gln Ala 
145                 150                 155                 160 
Leu Lys Lys Tyr Thr Val Asp Leu Thr Glu Arg Ala Glu Gln Gly Lys 
                165                 170                 175     
Leu Asp Pro Val Ile Gly Arg Asp Glu Glu Ile Arg Arg Thr Ile Gln 
            180                 185                 190         
Val Leu Gln Arg Arg Thr Lys Asn Asn Pro Val Leu Ile Gly Glu Pro 
        195                 200                 205             
Gly Val Gly Lys Thr Ala Ile Val Glu Gly Leu Ala Gln Arg Ile Ile 
    210                 215                 220                 
Asn Gly Glu Val Pro Glu Gly Leu Lys Asn Lys Arg Val Leu Ser Leu 
225                 230                 235                 240 
Asp Met Gly Ser Leu Ile Ala Gly Ala Lys Phe Arg Gly Glu Phe Glu 
                245                 250                 255     
Glu Arg Leu Lys Ala Val Leu Ser Asp Leu Ser Lys Gln Glu Gly Asn 
            260                 265                 270         
Val Ile Leu Phe Ile Asp Glu Leu His Thr Met Val Gly Ala Gly Lys 
        275                 280                 285             
Gly Asp Gly Ala Met Asp Ala Gly Asn Met Leu Lys Pro Ala Leu Ala 
    290                 295                 300                 
Arg Gly Glu Leu His Cys Val Gly Ala Thr Thr Leu Asp Glu Tyr Arg 
305                 310                 315                 320 
Gln Tyr Ile Glu Lys Asp Ala Ala Leu Glu Arg Arg Phe Gln Lys Val 
                325                 330                 335     
Phe Val Ala Glu Pro Thr Val Glu Asp Thr Ile Ala Ile Leu Arg Gly 
            340                 345                 350         
Leu Lys Glu Arg Tyr Glu Leu His His His Val Gln Ile Thr Asp Pro 
        355                 360                 365             
Ala Ile Val Ala Ala Ala Asn Leu Ser His Arg Tyr Ile Ala Asp Arg 
    370                 375                 380                 
Gln Leu Pro Asp Lys Ala Ile Asp Leu Ile Asp Glu Ala Ala Ser Ser 
385                 390                 395                 400 
Ile Arg Met Gln Met Asp Ser Lys Pro Glu Ser Leu Asp Arg Leu Glu 
                405                 410                 415     
Arg Arg Ile Ile Gln Leu Lys Leu Glu Gln Gln Ala Leu Asn Lys Glu 
            420                 425                 430         
Ser Asp Glu Ala Ser Lys Lys Arg Leu Glu Met Leu Asn Glu Glu Leu 
        435                 440                 445             
Glu His Lys Glu Arg Glu Tyr Ser Glu Leu Glu Glu Glu Trp Lys Ala 
    450                 455                 460                 
Glu Lys Ala Ser Leu Ser Gly Thr Gln Asn Ile Lys Ala Glu Ile Glu 
465                 470                 475                 480 
Gln Ala Lys Ile Ala Leu Glu Gln Ala Arg Arg Val Gly Asp Leu Ala 
                485                 490                 495     
Lys Met Ser Glu Ile Gln Tyr Gly Lys Leu Pro Gly Leu Glu Lys Gln 
            500                 505                 510         
Leu Glu Ala Ala Thr Gln Ser Glu Gly Lys Thr Met Lys Leu Leu Arg 
        515                 520                 525             
Asn Lys Val Thr Asp Val Glu Ile Ala Glu Val Leu Ala Arg Trp Thr 
    530                 535                 540                 
Gly Ile Pro Val Ser Arg Met Leu Glu Ser Glu Arg Ala Lys Leu Leu 
545                 550                 555                 560 
Arg Met Glu Asp Asp Leu His Glu Arg Val Ile Gly Gln Asn Glu Ala 
                565                 570                 575     
Val Glu Ala Val Ser Asn Ala Ile Arg Arg Ser Arg Ala Gly Leu Ser 
            580                 585                 590         
Asp Pro Asn Arg Pro Ile Gly Ser Phe Leu Phe Leu Gly Pro Thr Gly 
        595                 600                 605             
Val Gly Lys Thr Glu Leu Cys Lys Ala Leu Ala Asn Phe Leu Phe Asp 
    610                 615                 620                 
Ser Asp Asp Ala Met Val Arg Ile Asp Met Ser Glu Phe Met Glu Lys 
625                 630                 635                 640 
His Ser Val Ser Arg Leu Val Gly Ala Pro Pro Gly Tyr Val Gly Tyr 
                645                 650                 655     
Glu Glu Gly Gly Tyr Leu Thr Glu Ala Val Arg Arg Arg Pro Tyr Ser 
            660                 665                 670         
Val Ile Leu Leu Asp Glu Val Glu Lys Ala His Pro Asp Val Phe Asn 
        675                 680                 685             
Ile Leu Leu Gln Val Leu Asp Asp Gly Arg Leu Thr Asp Gly Gln Gly 
    690                 695                 700                 
Arg Thr Val Asp Phe Arg Asn Thr Val Val Ile Met Thr Ser Asn Leu 
705                 710                 715                 720 
Gly Ser Asp Leu Ile Gln Asp Arg Phe Gly Gln Leu Asp Tyr Gly Glu 
                725                 730                 735     
Met Lys Glu Leu Val Met Ser Val Val Ser His His Phe Arg Pro Glu 
            740                 745                 750         
Phe Ile Asn Arg Ile Asp Glu Thr Val Val Phe His Pro Leu Asp Gln 
        755                 760                 765             
Lys Asn Ile Lys Ala Ile Ala Arg Ile Gln Leu Glu Arg Leu Tyr Gln 
    770                 775                 780                 
Arg Leu Glu Glu Lys Gly Tyr Ser Val Thr Ile Thr Asp Ala Ala Leu 
785                 790                 795                 800 
Glu Gln Leu Ser Lys Ala Gly Phe Asp Pro Val Tyr Gly Ala Arg Pro 
                805                 810                 815     
Leu Lys Arg Ala Ile Gln Gln Glu Ile Glu Asn Pro Leu Ala Gln Gln 
            820                 825                 830         
Ile Leu Ser Gly Lys Leu Ile Pro Gly Lys Asp Ile Thr Leu Asp Val 
        835                 840                 845             
Ala Asp Glu Gln Ile Val Ala Lys Gln 
    850                 855         

<210> 2
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> ClpB fragment

<220> 
<221> SITE
<222> 1
<223> X is E or D

<220> 
<221> SITE
<222> 2
<223> X is 2, 3 or 4 of any amino acid

<400> 2
Xaa Xaa Arg Trp 
1               

<210> 3
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> ClpB fragment

<220> 
<221> SITE
<222> 1
<223> X is E or D

<220> 
<221> SITE
<222> 3
<223> X is any amino acid

<220> 
<221> SITE
<222> 4
<223> X is any amino acid

<400> 3
Xaa Val Xaa Xaa Arg Trp 
1               5       

<210> 4
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> ClpB fragment

<220> 
<221> SITE
<222> 1
<223> X is E or D

<400> 4
Xaa Val Leu Ala Arg Trp 
1               5       

<210> 5
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> ClpB fragment

<400> 5
Glu Val Leu Ala Arg Trp 
1               5       

<210> 6
<211> 9
<212> PRT
<213> Artificial Sequence


<220> 
<223> ClpB fragment

<220> 
<221> SITE
<222> 1
<223> X is E or D

<220> 
<221> SITE
<222> 2
<223> X is 2, 3 or 4 of any amino acid

<220> 
<221> SITE
<222> 5
<223> X is any amino acid

<220> 
<221> SITE
<222> 7
<223> X is any amino acid

<400> 6
Xaa Xaa Arg Trp Xaa Gly Xaa Pro Val 
1               5                   

<210> 7
<211> 9
<212> PRT
<213> Artificial Sequence


<220> 
<223> ClpB fragment

<220> 
<221> SITE
<222> 1
<223> X is E or D

<220> 
<221> SITE
<222> 2
<223> X is 2, 3 or 4 of any amino acid

<220> 
<221> SITE
<222> 5
<223> X is any amino acid

<220> 
<221> SITE
<222> 7
<223> X is I or K

<400> 7
Xaa Xaa Arg Trp Xaa Gly Xaa Pro Val 
1               5                   

<210> 8
<211> 9
<212> PRT
<213> Artificial Sequence


<220> 
<223> ClpB fragment

<220> 
<221> SITE
<222> 2
<223> X is 2, 3 or 4 of any amino acid

<220> 
<221> SITE
<222> 5
<223> X is any amino acid

<220> 
<221> SITE
<222> 7
<223> X is I or K

<400> 8
Glu Xaa Arg Trp Xaa Gly Xaa Pro Val 
1               5                   

<210> 9
<211> 9
<212> PRT
<213> Artificial Sequence


<220> 
<223> ClpB fragment

<220> 
<221> SITE
<222> 2
<223> X is 2, 3 or 4 of any amino acid

<220> 
<221> SITE
<222> 5
<223> X is any amino acid

<400> 9
Glu Xaa Arg Trp Xaa Gly Ile Pro Val 
1               5                   

<210> 10
<211> 9
<212> PRT
<213> Artificial Sequence


<220> 
<223> ClpB fragment

<220> 
<221> SITE
<222> 2
<223> X is 2, 3 or 4 of any amino acid

<400> 10
Glu Xaa Arg Trp Thr Gly Ile Pro Val 
1               5                   

<210> 11
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> ClpB fragment

<220> 
<221> SITE
<222> 3
<223> X is any amino acid

<220> 
<221> SITE
<222> 4
<223> X is any amino acid

<400> 11
Glu Val Xaa Xaa Arg Trp Thr Gly Ile Pro Val 
1               5                   10      

<210> 12
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> ClpB fragment

<400> 12
Glu Val Leu Ala Arg Trp Thr Gly Ile Pro Val 
1               5                   10      

<210> 13
<211> 9
<212> PRT
<213> Artificial Sequence


<220> 
<223> ClpB fragment

<220> 
<221> SITE
<222> 2
<223> X is 2, 3 or 4 of any amino acid

<220> 
<221> SITE
<222> 5
<223> X is any amino acid

<400> 13
Glu Xaa Arg Trp Xaa Gly Lys Pro Val 
1               5                   

<210> 14
<211> 9
<212> PRT
<213> Artificial Sequence


<220> 
<223> ClpB fragment

<220> 
<221> SITE
<222> 2
<223> X is 2, 3 or 4 of any amino acid

<400> 14
Glu Xaa Arg Trp Thr Gly Lys Pro Val 
1               5                   

<210> 15
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> ClpB fragment

<220> 
<221> SITE
<222> 3
<223> X is amino acid

<220> 
<221> SITE
<222> 4
<223> X is any amino acid

<400> 15
Glu Val Xaa Xaa Arg Trp Thr Gly Lys Pro Val 
1               5                   10      

<210> 16
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> ClpB fragment

<400> 16
Glu Val Leu Ala Arg Trp Thr Gly Lys Pro Val 
1               5                   10      

<210> 17
<211> 15
<212> PRT
<213> Artificial Sequence


<220> 
<223> ClpB fragment

<400> 17
Val Glu Ile Ala Glu Val Leu Ala Arg Trp Thr Gly Ile Pro Val 
1               5                   10                  15  

<210> 18
<211> 13
<212> PRT
<213> Artificial Sequence


<220> 
<223> ClpB fragment

<400> 18
Ile Ala Glu Val Leu Ala Arg Trp Thr Gly Ile Pro Val 
1               5                   10              

<210> 19
<211> 15
<212> PRT
<213> Artificial Sequence


<220> 
<223> ClpB fragment

<400> 19
Ala Glu Ile Ala Glu Val Leu Ala Arg Trp Thr Gly Ile Pro Val 
1               5                   10                  15  

<210> 20
<211> 13
<212> PRT
<213> Homo sapiens


<220> 
<223> Alpha-MSH

<400> 20
Ser Tyr Ser Met Glu His Phe Arg Trp Gly Lys Pro Val 
1               5                   10              

<210> 21
<211> 857
<212> PRT
<213> Escherichia coli


<220> 
<223> ClpB

<400> 21
Met Arg Leu Asp Arg Leu Thr Asn Lys Phe Gln Leu Ala Leu Ala Asp 
1               5                   10                  15      
Ala Gln Ser Leu Ala Leu Gly His Asp Asn Gln Phe Ile Glu Pro Leu 
            20                  25                  30          
His Leu Met Ser Ala Leu Leu Asn Gln Glu Gly Gly Ser Val Ser Pro 
        35                  40                  45              
Leu Leu Thr Ser Ala Gly Ile Asn Ala Gly Gln Leu Arg Thr Asp Ile 
    50                  55                  60                  
Asn Gln Ala Leu Asn Arg Leu Pro Gln Val Glu Gly Thr Gly Gly Asp 
65                  70                  75                  80  
Val Gln Pro Ser Gln Asp Leu Val Arg Val Leu Asn Leu Cys Asp Lys 
                85                  90                  95      
Leu Ala Gln Lys Arg Gly Asp Asn Phe Ile Ser Ser Glu Leu Phe Val 
            100                 105                 110         
Leu Ala Ala Leu Glu Ser Arg Gly Thr Leu Ala Asp Ile Leu Lys Ala 
        115                 120                 125             
Ala Gly Ala Thr Thr Ala Asn Ile Thr Gln Ala Ile Glu Gln Met Arg 
    130                 135                 140                 
Gly Gly Glu Ser Val Asn Asp Gln Gly Ala Glu Asp Gln Arg Gln Ala 
145                 150                 155                 160 
Leu Lys Lys Tyr Thr Ile Asp Leu Thr Glu Arg Ala Glu Gln Gly Lys 
                165                 170                 175     
Leu Asp Pro Val Ile Gly Arg Asp Glu Glu Ile Arg Arg Thr Ile Gln 
            180                 185                 190         
Val Leu Gln Arg Arg Thr Lys Asn Asn Pro Val Leu Ile Gly Glu Pro 
        195                 200                 205             
Gly Val Gly Lys Thr Ala Ile Val Glu Gly Leu Ala Gln Arg Ile Ile 
    210                 215                 220                 
Asn Gly Glu Val Pro Glu Gly Leu Lys Gly Arg Arg Val Leu Ala Leu 
225                 230                 235                 240 
Asp Met Gly Ala Leu Val Ala Gly Ala Lys Tyr Arg Gly Glu Phe Glu 
                245                 250                 255     
Glu Arg Leu Lys Gly Val Leu Asn Asp Leu Ala Lys Gln Glu Gly Asn 
            260                 265                 270         
Val Ile Leu Phe Ile Asp Glu Leu His Thr Met Val Gly Ala Gly Lys 
        275                 280                 285             
Ala Asp Gly Ala Met Asp Ala Gly Asn Met Leu Lys Pro Ala Leu Ala 
    290                 295                 300                 
Arg Gly Glu Leu His Cys Val Gly Ala Thr Thr Leu Asp Glu Tyr Arg 
305                 310                 315                 320 
Gln Tyr Ile Glu Lys Asp Ala Ala Leu Glu Arg Arg Phe Gln Lys Val 
                325                 330                 335     
Phe Val Ala Glu Pro Ser Val Glu Asp Thr Ile Ala Ile Leu Arg Gly 
            340                 345                 350         
Leu Lys Glu Arg Tyr Glu Leu His His His Val Gln Ile Thr Asp Pro 
        355                 360                 365             
Ala Ile Val Ala Ala Ala Thr Leu Ser His Arg Tyr Ile Ala Asp Arg 
    370                 375                 380                 
Gln Leu Pro Asp Lys Ala Ile Asp Leu Ile Asp Glu Ala Ala Ser Ser 
385                 390                 395                 400 
Ile Arg Met Gln Ile Asp Ser Lys Pro Glu Glu Leu Asp Arg Leu Asp 
                405                 410                 415     
Arg Arg Ile Ile Gln Leu Lys Leu Glu Gln Gln Ala Leu Met Lys Glu 
            420                 425                 430         
Ser Asp Glu Ala Ser Lys Lys Arg Leu Asp Met Leu Asn Glu Glu Leu 
        435                 440                 445             
Ser Asp Lys Glu Arg Gln Tyr Ser Glu Leu Glu Glu Glu Trp Lys Ala 
    450                 455                 460                 
Glu Lys Ala Ser Leu Ser Gly Thr Gln Thr Ile Lys Ala Glu Leu Glu 
465                 470                 475                 480 
Gln Ala Lys Ile Ala Ile Glu Gln Ala Arg Arg Val Gly Asp Leu Ala 
                485                 490                 495     
Arg Met Ser Glu Leu Gln Tyr Gly Lys Ile Pro Glu Leu Glu Lys Gln 
            500                 505                 510         
Leu Glu Ala Ala Thr Gln Leu Glu Gly Lys Thr Met Arg Leu Leu Arg 
        515                 520                 525             
Asn Lys Val Thr Asp Ala Glu Ile Ala Glu Val Leu Ala Arg Trp Thr 
    530                 535                 540                 
Gly Ile Pro Val Ser Arg Met Met Glu Ser Glu Arg Glu Lys Leu Leu 
545                 550                 555                 560 
Arg Met Glu Gln Glu Leu His His Arg Val Ile Gly Gln Asn Glu Ala 
                565                 570                 575     
Val Asp Ala Val Ser Asn Ala Ile Arg Arg Ser Arg Ala Gly Leu Ala 
            580                 585                 590         
Asp Pro Asn Arg Pro Ile Gly Ser Phe Leu Phe Leu Gly Pro Thr Gly 
        595                 600                 605             
Val Gly Lys Thr Glu Leu Cys Lys Ala Leu Ala Asn Phe Met Phe Asp 
    610                 615                 620                 
Ser Asp Glu Ala Met Val Arg Ile Asp Met Ser Glu Phe Met Glu Lys 
625                 630                 635                 640 
His Ser Val Ser Arg Leu Val Gly Ala Pro Pro Gly Tyr Val Gly Tyr 
                645                 650                 655     
Glu Glu Gly Gly Tyr Leu Thr Glu Ala Val Arg Arg Arg Pro Tyr Ser 
            660                 665                 670         
Val Ile Leu Leu Asp Glu Val Glu Lys Ala His Pro Asp Val Phe Asn 
        675                 680                 685             
Ile Leu Leu Gln Val Leu Asp Asp Gly Arg Leu Thr Asp Gly Gln Gly 
    690                 695                 700                 
Arg Thr Val Asp Phe Arg Asn Thr Val Val Ile Met Thr Ser Asn Leu 
705                 710                 715                 720 
Gly Ser Asp Leu Ile Gln Glu Arg Phe Gly Glu Leu Asp Tyr Ala His 
                725                 730                 735     
Met Lys Glu Leu Val Leu Gly Val Val Ser His Asn Phe Arg Pro Glu 
            740                 745                 750         
Phe Ile Asn Arg Ile Asp Glu Val Val Val Phe His Pro Leu Gly Glu 
        755                 760                 765             
Gln His Ile Ala Ser Ile Ala Gln Ile Gln Leu Lys Arg Leu Tyr Lys 
    770                 775                 780                 
Arg Leu Glu Glu Arg Gly Tyr Glu Ile His Ile Ser Asp Glu Ala Leu 
785                 790                 795                 800 
Lys Leu Leu Ser Glu Asn Gly Tyr Asp Pro Val Tyr Gly Ala Arg Pro 
                805                 810                 815     
Leu Lys Arg Ala Ile Gln Gln Gln Ile Glu Asn Pro Leu Ala Gln Gln 
            820                 825                 830         
Ile Leu Ser Gly Glu Leu Val Pro Gly Lys Val Ile Arg Leu Glu Val 
        835                 840                 845             
Asn Glu Asp Arg Ile Val Ala Val Gln 
    850                 855         

<210> 22
<211> 220
<212> PRT
<213> Artificial Sequence


<220> 
<223> ClpB fragment

<400> 22
Ala Glu Val Leu Ala Arg Trp Thr Gly Ile Pro Val Ser Arg Met Met
1               5                   10                  15     
Glu Ser Glu Arg Glu Lys Leu Leu Arg Met Glu Gln Glu Leu His His 
            20                  25                  30          
Arg Val Ile Gly Gln Asn Glu Ala Val Asp Ala Val Ser Asn Ala Ile 
        35                  40                  45               
Arg Arg Ser Arg Ala Gly Leu Ala Asp Pro Asn Arg Pro Ile Gly Ser 
    50                  55                  60                  
Phe Leu Phe Leu Gly Pro Thr Gly Val Gly Lys Thr Glu Leu Cys Lys
65                  70                  75                  80  
Ala Leu Ala Asn Phe Met Phe Asp Ser Asp Glu Ala Met Val Arg Ile 
                85                  90                  95      
Asp Met Ser Glu Phe Met Glu Lys His Ser Val Ser Arg Leu Val Gly 
            100                 105                 110          
Ala Pro Pro Gly Tyr Val Gly Tyr Glu Glu Gly Gly Tyr Leu Thr Glu 
        115                 120                 125             
Ala Val Arg Arg Arg Pro Tyr Ser Val Ile Leu Leu Asp Glu Val Glu 
    130                 135                 140                 
Lys Ala His Pro Asp Val Phe Asn Ile Leu Leu Gln Val Leu Asp Asp 
145                 150                 155                 160
Gly Arg Leu Thr Asp Gly Gln Gly Arg Thr Val Asp Phe Arg Asn Thr 
                165                 170                 175      
Val Val Ile Met Thr Ser Asn Leu Gly Ser Asp Leu Ile Gln Glu Arg 
            180                 185                 190        
Phe Gly Glu Leu Asp Tyr Ala His Met Lys Glu Leu Val Leu Gly Val 
        195                 200                 205             
Val Ser His Asn Phe Arg Pro Glu Phe Ile Asn Arg
    210                 215                 220   