                         SEQUENCE LISTING

<110>  Children's Hospital Medical Center
 
<120>  VLP-BASED BIVALENT EBOLA VACCINES AND METHODS OF MAKING AND USING
        SAME

<130>  0010872.0656611

<140>  PCT/US2018/024747
<141>  2018-03-28

<150>  62/477,480
<151>  2017-03-28

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  1503
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized HIV-1 Gag sequence

<400>  1
atgggcgccc gcgccagcgt gctgagcggc ggcgagctgg accgctggga gaagatccgc       60

ctgcgccccg gcggcaagaa gaagtacaag ctgaagcaca tcgtgtgggc cagccgcgag      120

ctggagcgct tcgccgtgaa ccccggcctg ctggagacca gcgagggctg ccgccagatc      180

ctgggccagc tgcagcccag cctgcagacc ggcagcgagg agctgcgcag cctgtacaac      240

accgtggcca ccctgtactg cgtgcaccag cgcatcgaga tcaaggacac caaggaggcc      300

ctggacaaga tcgaggagga gcagaacaag agcaagaaga aggcccagca ggccgccgcc      360

gacaccggcc acagcaacca ggtgagccag aactacccca tcgtgcagaa catccagggc      420

cagatggtgc accaggccat cagcccccgc accctgaacg cctgggtgaa ggtggtggag      480

gagaaggcct tcagccccga ggtgatcccc atgttcagcg ccctgagcga gggcgccacc      540

ccccaggacc tgaacaccat gctgaacacc gtgggcggcc accaggccgc catgcagatg      600

ctgaaggaga ccatcaacga ggaggccgcc gagtgggacc gcgtgcaccc cgtgcacgcc      660

ggccccatcg cccccggcca gatgcgcgag ccccgcggca gcgacatcgc cggcaccacc      720

agcaccctgc aggagcagat cggctggatg accaacaacc cccccatccc cgtgggcgag      780

atctacaagc gctggatcat cctgggcctg aacaagatcg tgcgcatgta cagccccacc      840

agcatcctgg acatccgcca gggccccaag gagcccttcc gcgactacgt ggaccgcttc      900

tacaagaccc tgcgcgccga gcaggccagc caggaggtga agaactggat gaccgagacc      960

ctgctggtgc agaacgccaa ccccgactgc aagaccatcc tgaaggccct gggccccgcc     1020

gccaccctgg aggagatgat gaccgcctgc cagggcgtgg gcggccccgg ccacaaggcc     1080

cgcgtgctgg ccgaggccat gagccaggtg accaacagcg ccaccatcat gatgcagcgc     1140

ggcaacttcc gcaaccagcg caagatcgtg aagtgcttca actgcggcaa ggagggccac     1200

accgcccgca actgccgcgc cccccgcaag aagggctgct ggaagtgcgg caaggagggc     1260

caccagatga aggactgcac cgagcgacag gctaattttt tagggaagat ctggccttcc     1320

cacaagggaa ggccagggaa ttttcttcag agcagaccag agccaacagc cccaccagaa     1380

gagagcttca ggtttgggga agagacaaca actccctctc agaagcagga gccgatagac     1440

aaggaactgt atcctttagc ttccctcaga tcactctttg gcagcgaccc ctcgtcacaa     1500

taa                                                                   1503


<210>  2
<211>  2035
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EBOV GP

<400>  2
atgggcgtca ctggtattct gcagctgccc cgagataggt tcaagcggac ttccttcttc       60

ctgtgggtca tcattctgtt tcagcggact ttcagcatcc ctctgggcgt gattcacaac      120

tcaaccctgc aggtgagcga cgtggataag ctggtctgtc gcgacaaact gagctccacc      180

aatcagctgc gatccgtggg actgaatctg gagggtaacg gagtggcaac tgatgtccca      240

agcgccacca aacggtgggg gtttaggtcc ggtgtgcccc ctaaggtggt caactacgag      300

gctggcgaat gggcagagaa ttgctataac ctggaaatca agaaacctga cggcagcgag      360

tgtctgccag cagctcctga tggcattagg ggattcccta ggtgcagata cgtgcacaaa      420

gtctctggaa ccgggccatg tgccggagac ttcgcttttc ataaggaagg ggcattcttt      480

ctgtacgatc gactggcctc caccgtgatc tatcggggaa ccacattcgc tgagggggtg      540

gtcgcatttc tgattctgcc ccaggctaag aaagacttct tttctagtca cccactgcgc      600

gaacccgtga acgcaaccga ggacccctca agcggctact atagtactac catccgatac      660

caggccacag gtttcggcac aaatgagact gaatacctgt ttgaagtgga caacctgact      720

tatgtccagc tggagagcag gttcacccct cagtttctgc tgcagctgaa cgaaaccatc      780

tatacaagcg gcaagcggag caatacaact ggcaagctga tttggaaagt gaacccagag      840

atcgatacca caattggcga atgggccttt tgggagacaa agaaaaatct gactcgcaaa      900

atccgatctg aggaactgag tttcaccgtg gtctccaatg gtgctaagaa cattagtggc      960

cagtcaccag cacgcacatc ctctgacccc gggactaata ctaccacaga agatcacaag     1020

atcatggcat ctgagaacag ttcagccatg gtgcaggtcc acagtcaggg acgagaggca     1080

gccgtgtcac atctgactac cctggccact atctctacca gtccacagtc tctgacaact     1140

aaacctggac cagacaatag tacacataac actcccgtgt acaagctgga tattagtgaa     1200

gccacacagg tcgagcagca ccatcggagg actgacaacg atagcaccgc ttccgacaca     1260

ccatcagcaa ccacagctgc aggcccaccc aaagctgaga ataccaacac atcaaagagc     1320

actgacttcc tggaccccgc cactaccaca tccccacaga atcactctga gacagctgga     1380

aacaataaca cccaccatca ggacacaggg gaggaatctg ccagctccgg gaagctgggt     1440

ctgatcacta acaccattgc cggcgtggct ggactgatca ctggcggaag acgcacccga     1500

cgggaagcaa ttgtgaatgc ccagcctaag tgcaatccaa acctgcacta ctggactacc     1560

caggacgagg gagcagctat cggactggct tggattccct acttcgggcc tgcagccgaa     1620

ggtatctata ttgagggcct gatgcataat caggatgggc tgatctgtgg tctgcgccag     1680

ctggccaacg aaacaactca ggctctgcag ctgttcctga gagcaaccac agagctgcgc     1740

accttttcta tcctgaacag gaaggccatt gacttcctgc tgcagagatg gggaggtaca     1800

tgccacatcc tgggaccaga ctgctgtatt gagcctcatg attggactaa gaatatcacc     1860

gacaaaattg atcagatcat tcacgacttt gtggataaga cactgcctga tcagggtgac     1920

aatgataact ggtggactgg atggcgacag tggattcccg caggaattgg ggtgaccggc     1980

gtcatcattg cagtgatcgc cctgttttgc atttgtaaat tcgtgttttg agaat          2035


<210>  3
<211>  676
<212>  PRT
<213>  Ebolavirus Zaire ebolavirus

<400>  3

Met Gly Val Thr Gly Ile Leu Gln Leu Pro Arg Asp Arg Phe Lys Arg 
1               5                   10                  15      


Thr Ser Phe Phe Leu Trp Val Ile Ile Leu Phe Gln Arg Thr Phe Ser 
            20                  25                  30          


Ile Pro Leu Gly Val Ile His Asn Ser Thr Leu Gln Val Ser Asp Val 
        35                  40                  45              


Asp Lys Leu Val Cys Arg Asp Lys Leu Ser Ser Thr Asn Gln Leu Arg 
    50                  55                  60                  


Ser Val Gly Leu Asn Leu Glu Gly Asn Gly Val Ala Thr Asp Val Pro 
65                  70                  75                  80  


Ser Ala Thr Lys Arg Trp Gly Phe Arg Ser Gly Val Pro Pro Lys Val 
                85                  90                  95      


Val Asn Tyr Glu Ala Gly Glu Trp Ala Glu Asn Cys Tyr Asn Leu Glu 
            100                 105                 110         


Ile Lys Lys Pro Asp Gly Ser Glu Cys Leu Pro Ala Ala Pro Asp Gly 
        115                 120                 125             


Ile Arg Gly Phe Pro Arg Cys Arg Tyr Val His Lys Val Ser Gly Thr 
    130                 135                 140                 


Gly Pro Cys Ala Gly Asp Phe Ala Phe His Lys Glu Gly Ala Phe Phe 
145                 150                 155                 160 


Leu Tyr Asp Arg Leu Ala Ser Thr Val Ile Tyr Arg Gly Thr Thr Phe 
                165                 170                 175     


Ala Glu Gly Val Val Ala Phe Leu Ile Leu Pro Gln Ala Lys Lys Asp 
            180                 185                 190         


Phe Phe Ser Ser His Pro Leu Arg Glu Pro Val Asn Ala Thr Glu Asp 
        195                 200                 205             


Pro Ser Ser Gly Tyr Tyr Ser Thr Thr Ile Arg Tyr Gln Ala Thr Gly 
    210                 215                 220                 


Phe Gly Thr Asn Glu Thr Glu Tyr Leu Phe Glu Val Asp Asn Leu Thr 
225                 230                 235                 240 


Tyr Val Gln Leu Glu Ser Arg Phe Thr Pro Gln Phe Leu Leu Gln Leu 
                245                 250                 255     


Asn Glu Thr Ile Tyr Thr Ser Gly Lys Arg Ser Asn Thr Thr Gly Lys 
            260                 265                 270         


Leu Ile Trp Lys Val Asn Pro Glu Ile Asp Thr Thr Ile Gly Glu Trp 
        275                 280                 285             


Ala Phe Trp Glu Thr Lys Lys Asn Leu Thr Arg Lys Ile Arg Ser Glu 
    290                 295                 300                 


Glu Leu Ser Phe Thr Val Val Ser Asn Gly Ala Lys Asn Ile Ser Gly 
305                 310                 315                 320 


Gln Ser Pro Ala Arg Thr Ser Ser Asp Pro Gly Thr Asn Thr Thr Thr 
                325                 330                 335     


Glu Asp His Lys Ile Met Ala Ser Glu Asn Ser Ser Ala Met Val Gln 
            340                 345                 350         


Val His Ser Gln Gly Arg Glu Ala Ala Val Ser His Leu Thr Thr Leu 
        355                 360                 365             


Ala Thr Ile Ser Thr Ser Pro Gln Ser Leu Thr Thr Lys Pro Gly Pro 
    370                 375                 380                 


Asp Asn Ser Thr His Asn Thr Pro Val Tyr Lys Leu Asp Ile Ser Glu 
385                 390                 395                 400 


Ala Thr Gln Val Glu Gln His His Arg Arg Thr Asp Asn Asp Ser Thr 
                405                 410                 415     


Ala Ser Asp Thr Pro Ser Ala Thr Thr Ala Ala Gly Pro Pro Lys Ala 
            420                 425                 430         


Glu Asn Thr Asn Thr Ser Lys Ser Thr Asp Phe Leu Asp Pro Ala Thr 
        435                 440                 445             


Thr Thr Ser Pro Gln Asn His Ser Glu Thr Ala Gly Asn Asn Asn Thr 
    450                 455                 460                 


His His Gln Asp Thr Gly Glu Glu Ser Ala Ser Ser Gly Lys Leu Gly 
465                 470                 475                 480 


Leu Ile Thr Asn Thr Ile Ala Gly Val Ala Gly Leu Ile Thr Gly Gly 
                485                 490                 495     


Arg Arg Thr Arg Arg Glu Ala Ile Val Asn Ala Gln Pro Lys Cys Asn 
            500                 505                 510         


Pro Asn Leu His Tyr Trp Thr Thr Gln Asp Glu Gly Ala Ala Ile Gly 
        515                 520                 525             


Leu Ala Trp Ile Pro Tyr Phe Gly Pro Ala Ala Glu Gly Ile Tyr Ile 
    530                 535                 540                 


Glu Gly Leu Met His Asn Gln Asp Gly Leu Ile Cys Gly Leu Arg Gln 
545                 550                 555                 560 


Leu Ala Asn Glu Thr Thr Gln Ala Leu Gln Leu Phe Leu Arg Ala Thr 
                565                 570                 575     


Thr Glu Leu Arg Thr Phe Ser Ile Leu Asn Arg Lys Ala Ile Asp Phe 
            580                 585                 590         


Leu Leu Gln Arg Trp Gly Gly Thr Cys His Ile Leu Gly Pro Asp Cys 
        595                 600                 605             


Cys Ile Glu Pro His Asp Trp Thr Lys Asn Ile Thr Asp Lys Ile Asp 
    610                 615                 620                 


Gln Ile Ile His Asp Phe Val Asp Lys Thr Leu Pro Asp Gln Gly Asp 
625                 630                 635                 640 


Asn Asp Asn Trp Trp Thr Gly Trp Arg Gln Trp Ile Pro Ala Gly Ile 
                645                 650                 655     


Gly Val Thr Gly Val Ile Ile Ala Val Ile Ala Leu Phe Cys Ile Cys 
            660                 665                 670         


Lys Phe Val Phe 
        675     


<210>  4
<211>  676
<212>  PRT
<213>  Ebolavirus Sudan ebolavirus

<400>  4

Met Gly Gly Leu Ser Leu Leu Gln Leu Pro Arg Asp Lys Phe Arg Lys 
1               5                   10                  15      


Ser Ser Phe Phe Val Trp Val Ile Ile Leu Phe Gln Lys Ala Phe Ser 
            20                  25                  30          


Met Pro Leu Gly Val Val Thr Asn Ser Thr Leu Glu Val Thr Glu Ile 
        35                  40                  45              


Asp Gln Leu Val Cys Lys Asp His Leu Ala Ser Thr Asp Gln Leu Lys 
    50                  55                  60                  


Ser Val Gly Leu Asn Leu Glu Gly Ser Gly Val Ser Thr Asp Ile Pro 
65                  70                  75                  80  


Ser Ala Thr Lys Arg Trp Gly Phe Arg Ser Gly Val Pro Pro Lys Val 
                85                  90                  95      


Val Ser Tyr Glu Ala Gly Glu Trp Ala Glu Asn Cys Tyr Asn Leu Glu 
            100                 105                 110         


Ile Lys Lys Pro Asp Gly Ser Glu Cys Leu Pro Pro Pro Pro Asp Gly 
        115                 120                 125             


Val Arg Gly Phe Pro Arg Cys Arg Tyr Val His Lys Ala Gln Gly Thr 
    130                 135                 140                 


Gly Pro Cys Pro Gly Asp Tyr Ala Phe His Lys Asp Gly Ala Phe Phe 
145                 150                 155                 160 


Leu Tyr Asp Arg Leu Ala Ser Thr Val Ile Tyr Arg Gly Val Asn Phe 
                165                 170                 175     


Ala Glu Gly Val Ile Ala Phe Leu Ile Leu Ala Lys Pro Lys Glu Thr 
            180                 185                 190         


Phe Leu Gln Ser Pro Pro Ile Arg Glu Ala Val Asn Tyr Thr Glu Asn 
        195                 200                 205             


Thr Ser Ser Tyr Tyr Ala Thr Ser Tyr Leu Glu Tyr Glu Ile Glu Asn 
    210                 215                 220                 


Phe Gly Ala Gln His Ser Thr Thr Leu Phe Lys Ile Asp Asn Asn Thr 
225                 230                 235                 240 


Phe Val Arg Leu Asp Arg Pro His Thr Pro Gln Phe Leu Phe Gln Leu 
                245                 250                 255     


Asn Asp Thr Ile His Leu His Gln Gln Leu Ser Asn Thr Thr Gly Arg 
            260                 265                 270         


Leu Ile Trp Thr Leu Asp Ala Asn Ile Asn Ala Asp Ile Gly Glu Trp 
        275                 280                 285             


Ala Phe Trp Glu Asn Lys Lys Asn Leu Ser Glu Gln Leu Arg Gly Glu 
    290                 295                 300                 


Glu Leu Ser Phe Glu Ala Leu Ser Leu Asn Glu Thr Glu Asp Asp Asp 
305                 310                 315                 320 


Ala Ala Ser Ser Arg Ile Thr Lys Gly Arg Ile Ser Asp Arg Ala Thr 
                325                 330                 335     


Arg Lys Tyr Ser Asp Leu Val Pro Lys Asn Ser Pro Gly Met Val Pro 
            340                 345                 350         


Leu His Ile Pro Glu Gly Glu Thr Thr Leu Pro Ser Gln Asn Ser Thr 
        355                 360                 365             


Glu Gly Arg Arg Val Gly Val Asn Thr Gln Glu Thr Ile Thr Glu Thr 
    370                 375                 380                 


Ala Ala Thr Ile Ile Gly Thr Asn Gly Asn His Met Gln Ile Ser Thr 
385                 390                 395                 400 


Ile Gly Ile Arg Pro Ser Ser Ser Gln Ile Pro Ser Ser Ser Pro Thr 
                405                 410                 415     


Thr Ala Pro Ser Pro Glu Ala Gln Thr Pro Thr Thr His Thr Ser Gly 
            420                 425                 430         


Pro Ser Val Met Ala Thr Glu Glu Pro Thr Thr Pro Pro Gly Ser Ser 
        435                 440                 445             


Pro Gly Pro Thr Thr Glu Ala Pro Thr Leu Thr Thr Pro Glu Asn Ile 
    450                 455                 460                 


Thr Thr Ala Val Lys Thr Val Leu Pro Gln Glu Ser Thr Ser Asn Gly 
465                 470                 475                 480 


Leu Ile Thr Ser Thr Val Thr Gly Ile Leu Gly Ser Leu Gly Leu Arg 
                485                 490                 495     


Lys Arg Ser Arg Arg Gln Thr Asn Thr Lys Ala Thr Gly Lys Cys Asn 
            500                 505                 510         


Pro Asn Leu His Tyr Trp Thr Ala Gln Glu Gln His Asn Ala Ala Gly 
        515                 520                 525             


Ile Ala Trp Ile Pro Tyr Phe Gly Pro Gly Ala Glu Gly Ile Tyr Thr 
    530                 535                 540                 


Glu Gly Leu Met His Asn Gln Asn Ala Leu Val Cys Gly Leu Arg Gln 
545                 550                 555                 560 


Leu Ala Asn Glu Thr Thr Gln Ala Leu Gln Leu Phe Leu Arg Ala Thr 
                565                 570                 575     


Thr Glu Leu Arg Thr Tyr Thr Ile Leu Asn Arg Lys Ala Ile Asp Phe 
            580                 585                 590         


Leu Leu Arg Arg Trp Gly Gly Thr Cys Arg Ile Leu Gly Pro Asp Cys 
        595                 600                 605             


Cys Ile Glu Pro His Asp Trp Thr Lys Asn Ile Thr Asp Lys Ile Asn 
    610                 615                 620                 


Gln Ile Ile His Asp Phe Ile Asp Asn Pro Leu Pro Asn Gln Asp Asn 
625                 630                 635                 640 


Asp Asp Asn Trp Trp Thr Gly Trp Arg Gln Trp Ile Pro Ala Gly Ile 
                645                 650                 655     


Gly Ile Thr Gly Ile Ile Ile Ala Ile Ile Ala Leu Leu Cys Val Cys 
            660                 665                 670         


Lys Leu Leu Cys 
        675     


