                         SEQUENCE LISTING

<110>  Children's Hospital Medical Research Foundation
 
<120>  Norovirus S Particle Based Vaccines and Methods of Making and 
       Using

<130>  0010872.0656608

<140>  PCT/US18/022552
<141>  2018-03-15

<150>  62/477,481
<151>  2017-03-28

<160>  51    

<170>  PatentIn version 3.5

<210>  1
<211>  231
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SR69A protein with hinge, linker (GGGG), and the Hisx6 peptide

<400>  1

Met Lys Met Ala Ser Asn Asp Ala Ser Pro Ser Asp Gly Ser Thr Ala 
1               5                   10                  15      


Asn Leu Val Pro Glu Val Asn Asn Glu Val Met Ala Leu Glu Pro Val 
            20                  25                  30          


Val Gly Ala Ala Ile Ala Ala Pro Val Ala Gly Gln Gln Asn Val Ile 
        35                  40                  45              


Asp Pro Trp Ile Arg Asn Asn Phe Val Gln Ala Pro Gly Gly Glu Phe 
    50                  55                  60                  


Thr Val Ser Pro Arg Asn Ala Pro Gly Glu Ile Leu Trp Ser Ala Pro 
65                  70                  75                  80  


Leu Gly Pro Asp Leu Asn Pro Tyr Leu Ser His Leu Ala Arg Met Tyr 
                85                  90                  95      


Asn Gly Tyr Ala Gly Gly Phe Glu Val Gln Val Ile Leu Ala Gly Asn 
            100                 105                 110         


Ala Phe Thr Ala Gly Lys Val Ile Phe Ala Ala Val Pro Pro Asn Phe 
        115                 120                 125             


Pro Thr Glu Gly Leu Ser Pro Ser Gln Val Thr Met Phe Pro His Ile 
    130                 135                 140                 


Ile Val Asp Val Arg Gln Leu Glu Pro Val Leu Ile Pro Leu Pro Asp 
145                 150                 155                 160 


Val Arg Asn Asn Phe Tyr His Tyr Asn Gln Ser Asn Asp Ser Thr Ile 
                165                 170                 175     


Lys Leu Ile Ala Met Leu Tyr Thr Pro Leu Arg Ala Asn Asn Ala Gly 
            180                 185                 190         


Asp Asp Val Phe Thr Val Ser Cys Arg Val Leu Thr Arg Pro Ser Pro 
        195                 200                 205             


Asp Phe Asp Phe Ile Phe Leu Val Pro Pro Thr Val Glu Gly Gly Gly 
    210                 215                 220                 


Gly His His His His His His 
225                 230     


<210>  2
<211>  18
<212>  PRT
<213>  Norovirus Norwalk Virus

<400>  2

Glu Phe Thr Val Ser Pro Arg Asn Ala Pro Gly Glu Ile Leu Trp Ser 
1               5                   10                  15      


Ala Pro 
        


<210>  3
<211>  18
<212>  PRT
<213>  Norovirus Norwalk Virus

<400>  3

Glu Phe Thr Val Ser Pro Arg Asn Ala Pro Gly Glu Val Leu Leu Asn 
1               5                   10                  15      


Leu Pro 
        


<210>  4
<211>  18
<212>  PRT
<213>  Norovirus Norwalk Virus

<400>  4

Glu Phe Thr Val Ser Pro Arg Asn Ser Pro Gly Glu Ile Leu Leu Asn 
1               5                   10                  15      


Leu Glu 
        


<210>  5
<211>  18
<212>  PRT
<213>  Norovirus Norwalk Virus


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa can be any naturally occurring amino acid

<400>  5

Xaa Phe Thr Val Ser Pro Arg Asn Ser Pro Gly Glu Val Leu Leu Asn 
1               5                   10                  15      


Leu Glu 
        


<210>  6
<211>  18
<212>  PRT
<213>  Norovirus Norwalk Virus

<400>  6

Glu Phe Thr Val Ser Pro Arg Asn Ser Pro Gly Glu Ile Leu Leu Asn 
1               5                   10                  15      


Leu Glu 
        


<210>  7
<211>  18
<212>  PRT
<213>  Norovirus Norwalk Virus

<400>  7

Glu Phe Thr Ile Ser Pro Arg Asn Ser Pro Gly Glu Ile Leu Leu Asn 
1               5                   10                  15      


Met Glu 
        


<210>  8
<211>  18
<212>  PRT
<213>  Norovirus Norwalk Virus

<400>  8

Glu Phe Thr Val Ser Pro Arg Asn Ala Pro Gly Glu Val Leu Leu Asn 
1               5                   10                  15      


Leu Glu 
        


<210>  9
<211>  18
<212>  PRT
<213>  Norovirus Norwalk Virus

<400>  9

Glu Phe Thr Val Ser Pro Arg Asn Ser Pro Gly Glu Ile Leu Val Asn 
1               5                   10                  15      


Leu Glu 
        


<210>  10
<211>  18
<212>  PRT
<213>  Norovirus Norwalk Virus

<400>  10

Glu Phe Thr Val Ser Pro Arg Asn Ser Pro Gly Glu Val Leu Leu Asn 
1               5                   10                  15      


Leu Glu 
        


<210>  11
<211>  18
<212>  PRT
<213>  Norovirus Norwalk Virus

<400>  11

Glu Phe Thr Val Ser Pro Arg Asn Ser Pro Gly Glu Ile Leu Leu Asn 
1               5                   10                  15      


Leu Glu 
        


<210>  12
<211>  18
<212>  PRT
<213>  Norovirus Norwalk Virus

<400>  12

Glu Phe Thr Val Ser Pro Arg Asn Ser Pro Gly Glu Ile Leu Met Asn 
1               5                   10                  15      


Leu Glu 
        


<210>  13
<211>  18
<212>  PRT
<213>  Norovirus Norwalk Virus

<400>  13

Glu Phe Thr Val Ser Pro Arg Asn Ser Pro Gly Glu Ile Leu Leu Asn 
1               5                   10                  15      


Leu Glu 
        


<210>  14
<211>  18
<212>  PRT
<213>  Norovirus Norwalk Virus

<400>  14

Glu Phe Thr Val Ser Pro Arg Asn Ala Pro Gly Glu Ile Leu Leu Asp 
1               5                   10                  15      


Leu Glu 
        


<210>  15
<211>  18
<212>  PRT
<213>  Norovirus Norwalk Virus

<400>  15

Glu Phe Thr Val Ser Pro Arg Asn Ala Pro Gly Glu Ile Leu Leu Asp 
1               5                   10                  15      


Leu Glu 
        


<210>  16
<211>  18
<212>  PRT
<213>  Norovirus Norwalk Virus

<400>  16

Glu Phe Thr Val Ser Pro Arg Asn Ser Pro Gly Glu Val Leu Leu Asn 
1               5                   10                  15      


Leu Glu 
        


<210>  17
<211>  18
<212>  PRT
<213>  Norovirus Norwalk Virus

<400>  17

Glu Phe Thr Val Ser Pro Arg Asn Ser Pro Gly Glu Val Leu Leu Asn 
1               5                   10                  15      


Leu Glu 
        


<210>  18
<211>  18
<212>  PRT
<213>  Norovirus Norwalk Virus

<400>  18

Glu Phe Thr Val Ser Pro Arg Asn Ser Pro Gly Glu Met Leu Leu Asn 
1               5                   10                  15      


Leu Glu 
        


<210>  19
<211>  18
<212>  PRT
<213>  Norovirus Norwalk Virus

<400>  19

Glu Phe Thr Val Ser Pro Arg Asn Ala Pro Gly Glu Phe Leu Leu Asp 
1               5                   10                  15      


Leu Glu 
        


<210>  20
<211>  18
<212>  PRT
<213>  Norovirus Norwalk Virus

<400>  20

Glu Phe Thr Val Ser Pro Arg Asn Ala Pro Gly Glu Phe Leu Leu Asp 
1               5                   10                  15      


Leu Glu 
        


<210>  21
<211>  18
<212>  PRT
<213>  Norovirus Norwalk Virus

<400>  21

Glu Phe Thr Val Ser Pro Arg Asn Ser Pro Gly Glu Leu Leu Leu Asp 
1               5                   10                  15      


Leu Glu 
        


<210>  22
<211>  18
<212>  PRT
<213>  Norovirus Norwalk Virus

<400>  22

Glu Phe Thr Val Ser Pro Arg Asn Ser Pro Gly Glu Ile Leu Leu Asp 
1               5                   10                  15      


Leu Glu 
        


<210>  23
<211>  18
<212>  PRT
<213>  Norovirus Norwalk Virus

<400>  23

Glu Phe Thr Val Ser Pro Arg Asn Ala Pro Gly Glu Val Leu Ile Asp 
1               5                   10                  15      


Leu Glu 
        


<210>  24
<211>  663
<212>  DNA
<213>  Norovirus Norwalk virus

<400>  24
atgaagatgg cgtcgaatga cgccagccca tctgatgggt ccacagccaa cctcgtccca       60

gaggtcaaca atgaggttat ggctttggag cccgttgttg gtgccgctat tgcggcacct      120

gtggcgggcc aacaaaacgt aattgacccc tggattagga ataattttgt acaagcccct      180

ggtggagagt ttacagtatc ccctagaaac gctccaggtg agatactatg gagcgcgccc      240

ttgggccctg atttgaaccc ctacctttct catttggcca gaatgtacaa tggttatgca      300

ggtggttttg aagtgcaggt aatcctcgcg gggaacgcgt tcaccgccgg gaaagtcata      360

tttgcagcag tcccaccaaa ttttccaact gaaggcttga gccccagcca ggttactatg      420

ttcccccata taatagtaga tgttaggcaa ttggaacctg tgttgatccc cttacctgat      480

gttaggaata acttctatca ttacaatcaa tcaaatgatt ctaccattaa attgatagca      540

atgctgtata caccacttag ggctaataat gctggggatg atgtcttcac agtctcttgt      600

cgagtcctca cgaggccatc ccccgatttt gatttcatat tcttggtgcc acccacagtt      660

gaa                                                                    663


<210>  25
<211>  221
<212>  PRT
<213>  Norovirus Norwalk virus

<400>  25

Met Lys Met Ala Ser Asn Asp Ala Ser Pro Ser Asp Gly Ser Thr Ala 
1               5                   10                  15      


Asn Leu Val Pro Glu Val Asn Asn Glu Val Met Ala Leu Glu Pro Val 
            20                  25                  30          


Val Gly Ala Ala Ile Ala Ala Pro Val Ala Gly Gln Gln Asn Val Ile 
        35                  40                  45              


Asp Pro Trp Ile Arg Asn Asn Phe Val Gln Ala Pro Gly Gly Glu Phe 
    50                  55                  60                  


Thr Val Ser Pro Arg Asn Ala Pro Gly Glu Ile Leu Trp Ser Ala Pro 
65                  70                  75                  80  


Leu Gly Pro Asp Leu Asn Pro Tyr Leu Ser His Leu Ala Arg Met Tyr 
                85                  90                  95      


Asn Gly Tyr Ala Gly Gly Phe Glu Val Gln Val Ile Leu Ala Gly Asn 
            100                 105                 110         


Ala Phe Thr Ala Gly Lys Val Ile Phe Ala Ala Val Pro Pro Asn Phe 
        115                 120                 125             


Pro Thr Glu Gly Leu Ser Pro Ser Gln Val Thr Met Phe Pro His Ile 
    130                 135                 140                 


Ile Val Asp Val Arg Gln Leu Glu Pro Val Leu Ile Pro Leu Pro Asp 
145                 150                 155                 160 


Val Arg Asn Asn Phe Tyr His Tyr Asn Gln Ser Asn Asp Ser Thr Ile 
                165                 170                 175     


Lys Leu Ile Ala Met Leu Tyr Thr Pro Leu Arg Ala Asn Asn Ala Gly 
            180                 185                 190         


Asp Asp Val Phe Thr Val Ser Cys Arg Val Leu Thr Arg Pro Ser Pro 
        195                 200                 205             


Asp Phe Asp Phe Ile Phe Leu Val Pro Pro Thr Val Glu 
    210                 215                 220     


<210>  26
<211>  663
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SR69A sequence

<400>  26
atgaagatgg cgtcgaatga cgccagccca tctgatgggt ccacagccaa cctcgtccca       60

gaggtcaaca atgaggttat ggctttggag cccgttgttg gtgccgctat tgcggcacct      120

gtggcgggcc aacaaaacgt aattgacccc tggattagga ataattttgt acaagcccct      180

ggtggagagt ttacagtatc ccctgcaaac gctccaggtg agatactatg gagcgcgccc      240

ttgggccctg atttgaaccc ctacctttct catttggcca gaatgtacaa tggttatgca      300

ggtggttttg aagtgcaggt aatcctcgcg gggaacgcgt tcaccgccgg gaaagtcata      360

tttgcagcag tcccaccaaa ttttccaact gaaggcttga gccccagcca ggttactatg      420

ttcccccata taatagtaga tgttaggcaa ttggaacctg tgttgatccc cttacctgat      480

gttaggaata acttctatca ttacaatcaa tcaaatgatt ctaccattaa attgatagca      540

atgctgtata caccacttag ggctaataat gctggggatg atgtcttcac agtctcttgt      600

cgagtcctca cgaggccatc ccccgatttt gatttcatat tcttggtgcc acccacagtt      660

gaa                                                                    663


<210>  27
<211>  221
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SR69A sequence (protein)

<400>  27

Met Lys Met Ala Ser Asn Asp Ala Ser Pro Ser Asp Gly Ser Thr Ala 
1               5                   10                  15      


Asn Leu Val Pro Glu Val Asn Asn Glu Val Met Ala Leu Glu Pro Val 
            20                  25                  30          


Val Gly Ala Ala Ile Ala Ala Pro Val Ala Gly Gln Gln Asn Val Ile 
        35                  40                  45              


Asp Pro Trp Ile Arg Asn Asn Phe Val Gln Ala Pro Gly Gly Glu Phe 
    50                  55                  60                  


Thr Val Ser Pro Ala Asn Ala Pro Gly Glu Ile Leu Trp Ser Ala Pro 
65                  70                  75                  80  


Leu Gly Pro Asp Leu Asn Pro Tyr Leu Ser His Leu Ala Arg Met Tyr 
                85                  90                  95      


Asn Gly Tyr Ala Gly Gly Phe Glu Val Gln Val Ile Leu Ala Gly Asn 
            100                 105                 110         


Ala Phe Thr Ala Gly Lys Val Ile Phe Ala Ala Val Pro Pro Asn Phe 
        115                 120                 125             


Pro Thr Glu Gly Leu Ser Pro Ser Gln Val Thr Met Phe Pro His Ile 
    130                 135                 140                 


Ile Val Asp Val Arg Gln Leu Glu Pro Val Leu Ile Pro Leu Pro Asp 
145                 150                 155                 160 


Val Arg Asn Asn Phe Tyr His Tyr Asn Gln Ser Asn Asp Ser Thr Ile 
                165                 170                 175     


Lys Leu Ile Ala Met Leu Tyr Thr Pro Leu Arg Ala Asn Asn Ala Gly 
            180                 185                 190         


Asp Asp Val Phe Thr Val Ser Cys Arg Val Leu Thr Arg Pro Ser Pro 
        195                 200                 205             


Asp Phe Asp Phe Ile Phe Leu Val Pro Pro Thr Val Glu 
    210                 215                 220     


<210>  28
<211>  1152
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SR69A-VP8 chimeric protein (Nucleotide sequences of Sr69A-VP8)

<400>  28
atgaagatgg cgtcgaatga cgccagccca tctgatgggt ccacagccaa cctcgtccca       60

gaggtcaaca atgaggttat ggctttggag cccgttgttg gtgccgctat tgcggcacct      120

gtggcgggcc aacaaaacgt aattgacccc tggattagga ataattttgt acaagcccct      180

ggtggagagt ttacagtatc ccctgcaaac gctccaggtg agatactatg gagcgcgccc      240

ttgggccctg atttgaaccc ctacctttct catttggcca gaatgtacaa tggttatgca      300

ggtggttttg aagtgcaggt aatcctcgcg gggaacgcgt tcaccgccgg gaaagtcata      360

tttgcagcag tcccaccaaa ttttccaact gaaggcttga gccccagcca ggttactatg      420

ttcccccata taatagtaga tgttaggcaa ttggaacctg tgttgatccc cttacctgat      480

gttaggaata acttctatca ttacaatcaa tcaaatgatt ctaccattaa attgatagca      540

atgctgtata caccacttag ggctaataat gctggggatg atgtcttcac agtctcttgt      600

cgagtcctca cgaggccatc ccccgatttt gatttcatat tcttggtgcc acccacagtt      660

gaacatcacc atcacttaga tggtccttat caacctacta catttacacc acctactgat      720

tactggatac ttattaattc aaatacaaat ggagtagtat acgagagtac aaataatagt      780

gacttttgga ctgcagtcat tgctgttgaa ccgcacgtca atccagtaga tagacaatat      840

aatgtatttg gtgaaaataa acaatttaat gtaagaaatg attcagataa atggaagttt      900

ttagaaatgt ttagaggcag tagtcaaaat gacttttata atagacgtac actaacttct      960

gatactagac tcgtgggaat attaaaatat ggtggaagaa tatggacatt tcatggtgaa     1020

acaccgaggg ctactactga tagctcaaac actgcaaatt tgaacggtat atcaattaca     1080

attcattcag aattttatat tattccaagg tcccaagagt ctaagtgtaa tgaatatatt     1140

aacaacggtc ta                                                         1152


<210>  29
<211>  384
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SR69A-VP8 chimeric protein

<400>  29

Met Lys Met Ala Ser Asn Asp Ala Ser Pro Ser Asp Gly Ser Thr Ala 
1               5                   10                  15      


Asn Leu Val Pro Glu Val Asn Asn Glu Val Met Ala Leu Glu Pro Val 
            20                  25                  30          


Val Gly Ala Ala Ile Ala Ala Pro Val Ala Gly Gln Gln Asn Val Ile 
        35                  40                  45              


Asp Pro Trp Ile Arg Asn Asn Phe Val Gln Ala Pro Gly Gly Glu Phe 
    50                  55                  60                  


Thr Val Ser Pro Ala Asn Ala Pro Gly Glu Ile Leu Trp Ser Ala Pro 
65                  70                  75                  80  


Leu Gly Pro Asp Leu Asn Pro Tyr Leu Ser His Leu Ala Arg Met Tyr 
                85                  90                  95      


Asn Gly Tyr Ala Gly Gly Phe Glu Val Gln Val Ile Leu Ala Gly Asn 
            100                 105                 110         


Ala Phe Thr Ala Gly Lys Val Ile Phe Ala Ala Val Pro Pro Asn Phe 
        115                 120                 125             


Pro Thr Glu Gly Leu Ser Pro Ser Gln Val Thr Met Phe Pro His Ile 
    130                 135                 140                 


Ile Val Asp Val Arg Gln Leu Glu Pro Val Leu Ile Pro Leu Pro Asp 
145                 150                 155                 160 


Val Arg Asn Asn Phe Tyr His Tyr Asn Gln Ser Asn Asp Ser Thr Ile 
                165                 170                 175     


Lys Leu Ile Ala Met Leu Tyr Thr Pro Leu Arg Ala Asn Asn Ala Gly 
            180                 185                 190         


Asp Asp Val Phe Thr Val Ser Cys Arg Val Leu Thr Arg Pro Ser Pro 
        195                 200                 205             


Asp Phe Asp Phe Ile Phe Leu Val Pro Pro Thr Val Glu His His His 
    210                 215                 220                 


His Leu Asp Gly Pro Tyr Gln Pro Thr Thr Phe Thr Pro Pro Thr Asp 
225                 230                 235                 240 


Tyr Trp Ile Leu Ile Asn Ser Asn Thr Asn Gly Val Val Tyr Glu Ser 
                245                 250                 255     


Thr Asn Asn Ser Asp Phe Trp Thr Ala Val Ile Ala Val Glu Pro His 
            260                 265                 270         


Val Asn Pro Val Asp Arg Gln Tyr Asn Val Phe Gly Glu Asn Lys Gln 
        275                 280                 285             


Phe Asn Val Arg Asn Asp Ser Asp Lys Trp Lys Phe Leu Glu Met Phe 
    290                 295                 300                 


Arg Gly Ser Ser Gln Asn Asp Phe Tyr Asn Arg Arg Thr Leu Thr Ser 
305                 310                 315                 320 


Asp Thr Arg Leu Val Gly Ile Leu Lys Tyr Gly Gly Arg Ile Trp Thr 
                325                 330                 335     


Phe His Gly Glu Thr Pro Arg Ala Thr Thr Asp Ser Ser Asn Thr Ala 
            340                 345                 350         


Asn Leu Asn Gly Ile Ser Ile Thr Ile His Ser Glu Phe Tyr Ile Ile 
        355                 360                 365             


Pro Arg Ser Gln Glu Ser Lys Cys Asn Glu Tyr Ile Asn Asn Gly Leu 
    370                 375                 380                 


<210>  30
<211>  1152
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SR69A/V57C/M140C-VP8

<400>  30
atgaagatgg cgtcgaatga cgccagccca tctgatgggt ccacagccaa cctcgtccca       60

gaggtcaaca atgaggttat ggctttggag cccgttgttg gtgccgctat tgcggcacct      120

gtggcgggcc aacaaaacgt aattgacccc tggattagga ataatttttg tcaagcccct      180

ggtggagagt ttacagtatc ccctgcaaac gctccaggtg agatactatg gagcgcgccc      240

ttgggccctg atttgaaccc ctacctttct catttggcca gaatgtacaa tggttatgca      300

ggtggttttg aagtgcaggt aatcctcgcg gggaacgcgt tcaccgccgg gaaagtcata      360

tttgcagcag tcccaccaaa ttttccaact gaaggcttga gccccagcca ggttacttgt      420

ttcccccata taatagtaga tgttaggcaa ttggaacctg tgttgatccc cttacctgat      480

gttaggaata acttctatca ttacaatcaa tcaaatgatt ctaccattaa attgatagca      540

atgctgtata caccacttag ggctaataat gctggggatg atgtcttcac agtctcttgt      600

cgagtcctca cgaggccatc ccccgatttt gatttcatat tcttggtgcc acccacagtt      660

gaacatcacc atcacttaga tggtccttat caacctacta catttacacc acctactgat      720

tactggatac ttattaattc aaatacaaat ggagtagtat acgagagtac aaataatagt      780

gacttttgga ctgcagtcat tgctgttgaa ccgcacgtca atccagtaga tagacaatat      840

aatgtatttg gtgaaaataa acaatttaat gtaagaaatg attcagataa atggaagttt      900

ttagaaatgt ttagaggcag tagtcaaaat gacttttata atagacgtac actaacttct      960

gatactagac tcgtgggaat attaaaatat ggtggaagaa tatggacatt tcatggtgaa     1020

acaccgaggg ctactactga tagctcaaac actgcaaatt tgaacggtat atcaattaca     1080

attcattcag aattttatat tattccaagg tcccaagagt ctaagtgtaa tgaatatatt     1140

aacaacggtc ta                                                         1152


<210>  31
<211>  384
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequences of the SR69A/V57C/M140C-VP8

<400>  31

Met Lys Met Ala Ser Asn Asp Ala Ser Pro Ser Asp Gly Ser Thr Ala 
1               5                   10                  15      


Asn Leu Val Pro Glu Val Asn Asn Glu Val Met Ala Leu Glu Pro Val 
            20                  25                  30          


Val Gly Ala Ala Ile Ala Ala Pro Val Ala Gly Gln Gln Asn Val Ile 
        35                  40                  45              


Asp Pro Trp Ile Arg Asn Asn Phe Cys Gln Ala Pro Gly Gly Glu Phe 
    50                  55                  60                  


Thr Val Ser Pro Ala Asn Ala Pro Gly Glu Ile Leu Trp Ser Ala Pro 
65                  70                  75                  80  


Leu Gly Pro Asp Leu Asn Pro Tyr Leu Ser His Leu Ala Arg Met Tyr 
                85                  90                  95      


Asn Gly Tyr Ala Gly Gly Phe Glu Val Gln Val Ile Leu Ala Gly Asn 
            100                 105                 110         


Ala Phe Thr Ala Gly Lys Val Ile Phe Ala Ala Val Pro Pro Asn Phe 
        115                 120                 125             


Pro Thr Glu Gly Leu Ser Pro Ser Gln Val Thr Cys Phe Pro His Ile 
    130                 135                 140                 


Ile Val Asp Val Arg Gln Leu Glu Pro Val Leu Ile Pro Leu Pro Asp 
145                 150                 155                 160 


Val Arg Asn Asn Phe Tyr His Tyr Asn Gln Ser Asn Asp Ser Thr Ile 
                165                 170                 175     


Lys Leu Ile Ala Met Leu Tyr Thr Pro Leu Arg Ala Asn Asn Ala Gly 
            180                 185                 190         


Asp Asp Val Phe Thr Val Ser Cys Arg Val Leu Thr Arg Pro Ser Pro 
        195                 200                 205             


Asp Phe Asp Phe Ile Phe Leu Val Pro Pro Thr Val Glu His His His 
    210                 215                 220                 


His Leu Asp Gly Pro Tyr Gln Pro Thr Thr Phe Thr Pro Pro Thr Asp 
225                 230                 235                 240 


Tyr Trp Ile Leu Ile Asn Ser Asn Thr Asn Gly Val Val Tyr Glu Ser 
                245                 250                 255     


Thr Asn Asn Ser Asp Phe Trp Thr Ala Val Ile Ala Val Glu Pro His 
            260                 265                 270         


Val Asn Pro Val Asp Arg Gln Tyr Asn Val Phe Gly Glu Asn Lys Gln 
        275                 280                 285             


Phe Asn Val Arg Asn Asp Ser Asp Lys Trp Lys Phe Leu Glu Met Phe 
    290                 295                 300                 


Arg Gly Ser Ser Gln Asn Asp Phe Tyr Asn Arg Arg Thr Leu Thr Ser 
305                 310                 315                 320 


Asp Thr Arg Leu Val Gly Ile Leu Lys Tyr Gly Gly Arg Ile Trp Thr 
                325                 330                 335     


Phe His Gly Glu Thr Pro Arg Ala Thr Thr Asp Ser Ser Asn Thr Ala 
            340                 345                 350         


Asn Leu Asn Gly Ile Ser Ile Thr Ile His Ser Glu Phe Tyr Ile Ile 
        355                 360                 365             


Pro Arg Ser Gln Glu Ser Lys Cys Asn Glu Tyr Ile Asn Asn Gly Leu 
    370                 375                 380                 


<210>  32
<211>  1152
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SR69A/V57C/Q58C/S136C-VP8

<400>  32
atgaagatgg cgtcgaatga cgccagccca tctgatgggt ccacagccaa cctcgtccca       60

gaggtcaaca atgaggttat ggctttggag cccgttgttg gtgccgctat tgcggcacct      120

gtggcgggcc aacaaaacgt aattgacccc tggattagga ataatttttg ttgtgcccct      180

ggtggagagt ttacagtatc ccctgcaaac gctccaggtg agatactatg gagcgcgccc      240

ttgggccctg atttgaaccc ctacctttct catttggcca gaatgtacaa tggttatgca      300

ggtggttttg aagtgcaggt aatcctcgcg gggaacgcgt tcaccgccgg gaaagtcata      360

tttgcagcag tcccaccaaa ttttccaact gaaggcttga gcccctgtca ggttactatg      420

ttcccccata taatagtaga tgttaggcaa ttggaacctg tgttgatccc cttacctgat      480

gttaggaata acttctatca ttacaatcaa tcaaatgatt ctaccattaa attgatagca      540

atgctgtata caccacttag ggctaataat gctggggatg atgtcttcac agtctcttgt      600

cgagtcctca cgaggccatc ccccgatttt gatttcatat tcttggtgcc acccacagtt      660

gaacatcacc atcacttaga tggtccttat caacctacta catttacacc acctactgat      720

tactggatac ttattaattc aaatacaaat ggagtagtat acgagagtac aaataatagt      780

gacttttgga ctgcagtcat tgctgttgaa ccgcacgtca atccagtaga tagacaatat      840

aatgtatttg gtgaaaataa acaatttaat gtaagaaatg attcagataa atggaagttt      900

ttagaaatgt ttagaggcag tagtcaaaat gacttttata atagacgtac actaacttct      960

gatactagac tcgtgggaat attaaaatat ggtggaagaa tatggacatt tcatggtgaa     1020

acaccgaggg ctactactga tagctcaaac actgcaaatt tgaacggtat atcaattaca     1080

attcattcag aattttatat tattccaagg tcccaagagt ctaagtgtaa tgaatatatt     1140

aacaacggtc ta                                                         1152


<210>  33
<211>  385
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SR69A/V57C/Q58C/S136C-VP8

<400>  33

Met Lys Met Ala Ser Asn Asp Ala Ser Pro Ser Asp Gly Ser Thr Ala 
1               5                   10                  15      


Asn Leu Val Pro Glu Val Asn Asn Glu Val Met Ala Leu Glu Pro Val 
            20                  25                  30          


Val Gly Ala Ala Ile Ala Ala Pro Val Ala Gly Gln Gln Asn Val Ile 
        35                  40                  45              


Asp Pro Trp Ile Arg Asn Asn Phe Cys Cys Gln Ala Pro Gly Gly Glu 
    50                  55                  60                  


Phe Thr Val Ser Pro Ala Asn Ala Pro Gly Glu Ile Leu Trp Ser Ala 
65                  70                  75                  80  


Pro Leu Gly Pro Asp Leu Asn Pro Tyr Leu Ser His Leu Ala Arg Met 
                85                  90                  95      


Tyr Asn Gly Tyr Ala Gly Gly Phe Glu Val Gln Val Ile Leu Ala Gly 
            100                 105                 110         


Asn Ala Phe Thr Ala Gly Lys Val Ile Phe Ala Ala Val Pro Pro Asn 
        115                 120                 125             


Phe Pro Thr Glu Gly Leu Ser Pro Ser Gln Val Thr Cys Phe Pro His 
    130                 135                 140                 


Ile Ile Val Asp Val Arg Gln Leu Glu Pro Val Leu Ile Pro Leu Pro 
145                 150                 155                 160 


Asp Val Arg Asn Asn Phe Tyr His Tyr Asn Gln Ser Asn Asp Ser Thr 
                165                 170                 175     


Ile Lys Leu Ile Ala Met Leu Tyr Thr Pro Leu Arg Ala Asn Asn Ala 
            180                 185                 190         


Gly Asp Asp Val Phe Thr Val Ser Cys Arg Val Leu Thr Arg Pro Ser 
        195                 200                 205             


Pro Asp Phe Asp Phe Ile Phe Leu Val Pro Pro Thr Val Glu His His 
    210                 215                 220                 


His His Leu Asp Gly Pro Tyr Gln Pro Thr Thr Phe Thr Pro Pro Thr 
225                 230                 235                 240 


Asp Tyr Trp Ile Leu Ile Asn Ser Asn Thr Asn Gly Val Val Tyr Glu 
                245                 250                 255     


Ser Thr Asn Asn Ser Asp Phe Trp Thr Ala Val Ile Ala Val Glu Pro 
            260                 265                 270         


His Val Asn Pro Val Asp Arg Gln Tyr Asn Val Phe Gly Glu Asn Lys 
        275                 280                 285             


Gln Phe Asn Val Arg Asn Asp Ser Asp Lys Trp Lys Phe Leu Glu Met 
    290                 295                 300                 


Phe Arg Gly Ser Ser Gln Asn Asp Phe Tyr Asn Arg Arg Thr Leu Thr 
305                 310                 315                 320 


Ser Asp Thr Arg Leu Val Gly Ile Leu Lys Tyr Gly Gly Arg Ile Trp 
                325                 330                 335     


Thr Phe His Gly Glu Thr Pro Arg Ala Thr Thr Asp Ser Ser Asn Thr 
            340                 345                 350         


Ala Asn Leu Asn Gly Ile Ser Ile Thr Ile His Ser Glu Phe Tyr Ile 
        355                 360                 365             


Ile Pro Arg Ser Gln Glu Ser Lys Cys Asn Glu Tyr Ile Asn Asn Gly 
    370                 375                 380                 


Leu 
385 


<210>  34
<211>  477
<212>  DNA
<213>  Rotavirus Rotavirus A

<400>  34
ttagatggtc cttatcaacc tactacattt acaccaccta ctgattactg gatacttatt       60

aattcaaata caaatggagt agtatacgag agtacaaata atagtgactt ttggactgca      120

gtcattgctg ttgaaccgca cgtcaatcca gtagatagac aatataatgt atttggtgaa      180

aataaacaat ttaatgtaag aaatgattca gataaatgga agtttttaga aatgtttaga      240

ggcagtagtc aaaatgactt ttataataga cgtacactaa cttctgatac tagactcgtg      300

ggaatattaa aatatggtgg aagaatatgg acatttcatg gtgaaacacc gagggctact      360

actgatagct caaacactgc aaatttgaac ggtatatcaa ttacaattca ttcagaattt      420

tatattattc caaggtccca agagtctaag tgtaatgaat atattaacaa cggtcta         477


<210>  35
<211>  159
<212>  PRT
<213>  Rotavirus Rotavirus A

<400>  35

Leu Asp Gly Pro Tyr Gln Pro Thr Thr Phe Thr Pro Pro Thr Asp Tyr 
1               5                   10                  15      


Trp Ile Leu Ile Asn Ser Asn Thr Asn Gly Val Val Tyr Glu Ser Thr 
            20                  25                  30          


Asn Asn Ser Asp Phe Trp Thr Ala Val Ile Ala Val Glu Pro His Val 
        35                  40                  45              


Asn Pro Val Asp Arg Gln Tyr Asn Val Phe Gly Glu Asn Lys Gln Phe 
    50                  55                  60                  


Asn Val Arg Asn Asp Ser Asp Lys Trp Lys Phe Leu Glu Met Phe Arg 
65                  70                  75                  80  


Gly Ser Ser Gln Asn Asp Phe Tyr Asn Arg Arg Thr Leu Thr Ser Asp 
                85                  90                  95      


Thr Arg Leu Val Gly Ile Leu Lys Tyr Gly Gly Arg Ile Trp Thr Phe 
            100                 105                 110         


His Gly Glu Thr Pro Arg Ala Thr Thr Asp Ser Ser Asn Thr Ala Asn 
        115                 120                 125             


Leu Asn Gly Ile Ser Ile Thr Ile His Ser Glu Phe Tyr Ile Ile Pro 
    130                 135                 140                 


Arg Ser Gln Glu Ser Lys Cys Asn Glu Tyr Ile Asn Asn Gly Leu 
145                 150                 155                 


<210>  36
<211>  1152
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SR69A/V57C/Q58C/S136C/M140C-VP8 chimeric proteins (the expression
       construct was made by cloning these sequences into the pET-24 
       vector)

<400>  36
atgaagatgg cgtcgaatga cgccagccca tctgatgggt ccacagccaa cctcgtccca       60

gaggtcaaca atgaggttat ggctttggag cccgttgttg gtgccgctat tgcggcacct      120

gtggcgggcc aacaaaacgt aattgacccc tggattagga ataatttttg ttgtgcccct      180

ggtggagagt ttacagtatc ccctgcaaac gctccaggtg agatactatg gagcgcgccc      240

ttgggccctg atttgaaccc ctacctttct catttggcca gaatgtacaa tggttatgca      300

ggtggttttg aagtgcaggt aatcctcgcg gggaacgcgt tcaccgccgg gaaagtcata      360

tttgcagcag tcccaccaaa ttttccaact gaaggcttga gcccctgtca ggttacttgt      420

ttcccccata taatagtaga tgttaggcaa ttggaacctg tgttgatccc cttacctgat      480

gttaggaata acttctatca ttacaatcaa tcaaatgatt ctaccattaa attgatagca      540

atgctgtata caccacttag ggctaataat gctggggatg atgtcttcac agtctcttgt      600

cgagtcctca cgaggccatc ccccgatttt gatttcatat tcttggtgcc acccacagtt      660

gaacatcacc atcacttaga tggtccttat caacctacta catttacacc acctactgat      720

tactggatac ttattaattc aaatacaaat ggagtagtat acgagagtac aaataatagt      780

gacttttgga ctgcagtcat tgctgttgaa ccgcacgtca atccagtaga tagacaatat      840

aatgtatttg gtgaaaataa acaatttaat gtaagaaatg attcagataa atggaagttt      900

ttagaaatgt ttagaggcag tagtcaaaat gacttttata atagacgtac actaacttct      960

gatactagac tcgtgggaat attaaaatat ggtggaagaa tatggacatt tcatggtgaa     1020

acaccgaggg ctactactga tagctcaaac actgcaaatt tgaacggtat atcaattaca     1080

attcattcag aattttatat tattccaagg tcccaagagt ctaagtgtaa tgaatatatt     1140

aacaacggtc ta                                                         1152


<210>  37
<211>  385
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequences of the SR69A/V57C/Q58/S136C/M140C-VP8

<400>  37

Met Lys Met Ala Ser Asn Asp Ala Ser Pro Ser Asp Gly Ser Thr Ala 
1               5                   10                  15      


Asn Leu Val Pro Glu Val Asn Asn Glu Val Met Ala Leu Glu Pro Val 
            20                  25                  30          


Val Gly Ala Ala Ile Ala Ala Pro Val Ala Gly Gln Gln Asn Val Ile 
        35                  40                  45              


Asp Pro Trp Ile Arg Asn Asn Phe Cys Cys Gln Ala Pro Gly Gly Glu 
    50                  55                  60                  


Phe Thr Val Ser Pro Ala Asn Ala Pro Gly Glu Ile Leu Trp Ser Ala 
65                  70                  75                  80  


Pro Leu Gly Pro Asp Leu Asn Pro Tyr Leu Ser His Leu Ala Arg Met 
                85                  90                  95      


Tyr Asn Gly Tyr Ala Gly Gly Phe Glu Val Gln Val Ile Leu Ala Gly 
            100                 105                 110         


Asn Ala Phe Thr Ala Gly Lys Val Ile Phe Ala Ala Val Pro Pro Asn 
        115                 120                 125             


Phe Pro Thr Glu Gly Leu Ser Pro Ser Gln Val Thr Cys Phe Pro His 
    130                 135                 140                 


Ile Ile Val Asp Val Arg Gln Leu Glu Pro Val Leu Ile Pro Leu Pro 
145                 150                 155                 160 


Asp Val Arg Asn Asn Phe Tyr His Tyr Asn Gln Ser Asn Asp Ser Thr 
                165                 170                 175     


Ile Lys Leu Ile Ala Met Leu Tyr Thr Pro Leu Arg Ala Asn Asn Ala 
            180                 185                 190         


Gly Asp Asp Val Phe Thr Val Ser Cys Arg Val Leu Thr Arg Pro Ser 
        195                 200                 205             


Pro Asp Phe Asp Phe Ile Phe Leu Val Pro Pro Thr Val Glu His His 
    210                 215                 220                 


His His Leu Asp Gly Pro Tyr Gln Pro Thr Thr Phe Thr Pro Pro Thr 
225                 230                 235                 240 


Asp Tyr Trp Ile Leu Ile Asn Ser Asn Thr Asn Gly Val Val Tyr Glu 
                245                 250                 255     


Ser Thr Asn Asn Ser Asp Phe Trp Thr Ala Val Ile Ala Val Glu Pro 
            260                 265                 270         


His Val Asn Pro Val Asp Arg Gln Tyr Asn Val Phe Gly Glu Asn Lys 
        275                 280                 285             


Gln Phe Asn Val Arg Asn Asp Ser Asp Lys Trp Lys Phe Leu Glu Met 
    290                 295                 300                 


Phe Arg Gly Ser Ser Gln Asn Asp Phe Tyr Asn Arg Arg Thr Leu Thr 
305                 310                 315                 320 


Ser Asp Thr Arg Leu Val Gly Ile Leu Lys Tyr Gly Gly Arg Ile Trp 
                325                 330                 335     


Thr Phe His Gly Glu Thr Pro Arg Ala Thr Thr Asp Ser Ser Asn Thr 
            340                 345                 350         


Ala Asn Leu Asn Gly Ile Ser Ile Thr Ile His Ser Glu Phe Tyr Ile 
        355                 360                 365             


Ile Pro Arg Ser Gln Glu Ser Lys Cys Asn Glu Tyr Ile Asn Asn Gly 
    370                 375                 380                 


Leu 
385 


<210>  38
<211>  1152
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequences of S-VP8

<400>  38
atgaagatgg cgtcgaatga cgccagccca tctgatgggt ccacagccaa cctcgtccca       60

gaggtcaaca atgaggttat ggctttggag cccgttgttg gtgccgctat tgcggcacct      120

gtggcgggcc aacaaaacgt aattgacccc tggattagga ataattttgt acaagcccct      180

ggtggagagt ttacagtatc ccctagaaac gctccaggtg agatactatg gagcgcgccc      240

ttgggccctg atttgaaccc ctacctttct catttggcca gaatgtacaa tggttatgca      300

ggtggttttg aagtgcaggt aatcctcgcg gggaacgcgt tcaccgccgg gaaagtcata      360

tttgcagcag tcccaccaaa ttttccaact gaaggcttga gccccagcca ggttactatg      420

ttcccccata taatagtaga tgttaggcaa ttggaacctg tgttgatccc cttacctgat      480

gttaggaata acttctatca ttacaatcaa tcaaatgatt ctaccattaa attgatagca      540

atgctgtata caccacttag ggctaataat gctggggatg atgtcttcac agtctcttgt      600

cgagtcctca cgaggccatc ccccgatttt gatttcatat tcttggtgcc acccacagtt      660

gaacatcacc atcacttaga tggtccttat caacctacta catttacacc acctactgat      720

tactggatac ttattaattc aaatacaaat ggagtagtat acgagagtac aaataatagt      780

gacttttgga ctgcagtcat tgctgttgaa ccgcacgtca atccagtaga tagacaatat      840

aatgtatttg gtgaaaataa acaatttaat gtaagaaatg attcagataa atggaagttt      900

ttagaaatgt ttagaggcag tagtcaaaat gacttttata atagacgtac actaacttct      960

gatactagac tcgtgggaat attaaaatat ggtggaagaa tatggacatt tcatggtgaa     1020

acaccgaggg ctactactga tagctcaaac actgcaaatt tgaacggtat atcaattaca     1080

attcattcag aattttatat tattccaagg tcccaagagt ctaagtgtaa tgaatatatt     1140

aacaacggtc ta                                                         1152


<210>  39
<211>  383
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequences of S-VP8

<400>  39

Met Lys Met Ala Ser Asn Asp Ala Ser Pro Ser Asp Gly Ser Thr Ala 
1               5                   10                  15      


Asn Leu Val Pro Glu Val Asn Asn Glu Val Met Ala Leu Glu Pro Val 
            20                  25                  30          


Val Gly Ala Ala Ile Ala Ala Pro Val Ala Gly Gln Gln Asn Val Ile 
        35                  40                  45              


Asp Pro Trp Ile Arg Asn Asn Phe Val Gln Ala Pro Gly Gly Glu Phe 
    50                  55                  60                  


Thr Val Ser Pro Arg Asn Ala Pro Gly Glu Ile Leu Trp Ser Ala Pro 
65                  70                  75                  80  


Leu Gly Pro Asp Leu Asn Pro Tyr Leu Ser His Leu Ala Arg Met Tyr 
                85                  90                  95      


Asn Gly Tyr Ala Gly Gly Phe Glu Val Gln Val Ile Leu Ala Gly Asn 
            100                 105                 110         


Ala Phe Thr Ala Gly Lys Val Ile Phe Ala Ala Val Pro Pro Asn Phe 
        115                 120                 125             


Pro Thr Glu Gly Leu Ser Pro Ser Gln Val Thr Met Phe Pro His Ile 
    130                 135                 140                 


Ile Val Asp Val Arg Gln Leu Glu Pro Val Leu Ile Pro Leu Pro Asp 
145                 150                 155                 160 


Val Arg Asn Asn Phe Tyr His Tyr Asn Gln Ser Asn Asp Ser Thr Ile 
                165                 170                 175     


Lys Leu Ile Ala Met Leu Tyr Thr Pro Leu Arg Ala Asn Asn Ala Gly 
            180                 185                 190         


Asp Asp Val Phe Thr Val Ser Cys Arg Val Leu Thr Arg Pro Ser Pro 
        195                 200                 205             


Asp Phe Asp Phe Ile Phe Leu Val Pro Pro Thr Val Glu His His His 
    210                 215                 220                 


His Leu Asp Gly Pro Tyr Gln Pro Thr Thr Phe Thr Pro Pro Thr Asp 
225                 230                 235                 240 


Tyr Trp Ile Leu Ile Asn Ser Asn Thr Asn Gly Val Val Tyr Glu Ser 
                245                 250                 255     


Thr Asn Asn Ser Asp Phe Trp Thr Ala Val Ile Ala Val Glu Pro His 
            260                 265                 270         


Val Asn Pro Val Asp Arg Gln Tyr Asn Val Phe Gly Glu Asn Lys Gln 
        275                 280                 285             


Phe Asn Val Arg Asn Asp Ser Asp Lys Trp Lys Phe Leu Glu Phe Arg 
    290                 295                 300                 


Gly Ser Ser Gln Asn Asp Phe Tyr Asn Arg Arg Thr Leu Thr Ser Asp 
305                 310                 315                 320 


Thr Arg Leu Val Gly Ile Leu Lys Tyr Gly Gly Arg Ile Trp Thr Phe 
                325                 330                 335     


His Gly Glu Thr Pro Arg Ala Thr Thr Asp Ser Ser Asn Thr Ala Asn 
            340                 345                 350         


Leu Asn Gly Ile Ser Ile Thr Ile His Ser Glu Phe Tyr Ile Ile Pro 
        355                 360                 365             


Arg Ser Gln Glu Ser Lys Cys Asn Glu Tyr Ile Asn Asn Gly Leu 
    370                 375                 380             


<210>  40
<211>  1149
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequences of the SR69A/V57C/Q58C/S136C-mVP8 (the 
       expression construct was made by cloning these sequences into the
       pET-24 vector)

<400>  40
atgaagatgg cgtcgaatga cgccagccca tctgatgggt ccacagccaa cctcgtccca       60

gaggtcaaca atgaggttat ggctttggag cccgttgttg gtgccgctat tgcggcacct      120

gtggcgggcc aacaaaacgt aattgacccc tggattagga ataatttttg ttgtgcccct      180

ggtggagagt ttacagtatc ccctgcaaac gctccaggtg agatactatg gagcgcgccc      240

ttgggccctg atttgaaccc ctacctttct catttggcca gaatgtacaa tggttatgca      300

ggtggttttg aagtgcaggt aatcctcgcg gggaacgcgt tcaccgccgg gaaagtcata      360

tttgcagcag tcccaccaaa ttttccaact gaaggcttga gcccctgtca ggttactatg      420

ttcccccata taatagtaga tgttaggcaa ttggaacctg tgttgatccc cttacctgat      480

gttaggaata acttctatca ttacaatcaa tcaaatgatt ctaccattaa attgatagca      540

atgctgtata caccacttag ggctaataat gctggggatg atgtcttcac agtctcttgt      600

cgagtcctca cgaggccatc ccccgatttt gatttcatat tcttggtgcc acccacagtt      660

gaacatcacc atcaccttga tggaccatac caaccaatag cgttcagtcc gccgccagag      720

tactatattc tactctcccc gactgcaccc ggagtaatcg ctgaatgtac gaatactgtc      780

aaccgctgga tagcaatcat agctatagag ccaaacgtgt caccaacaaa tcgtacctac      840

acattgttcg gaattactga acagctaaca gtagaaaaca gctccgtgga taaatggaag      900

tttatagact tcatgaaaac tccaacaact ggcagctacg tccgttataa cattttgttg      960

tctagcacta agctatgcgc agtggcgaag cacacggaca atttatactc ctatgttgga     1020

gaaacgccta ctgcaggtca ggcatactac tcttctttca atatatttaa cctaaccgcg     1080

cactgtgact tctacattat accatggtcg cagcaatcgt tgtgcacgca atacgttaat     1140

aacggatta                                                             1149


<210>  41
<211>  383
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequences of the SR69A/V57C/Q58C/S136C-mVP8

<400>  41

Met Lys Met Ala Ser Asn Asp Ala Ser Pro Ser Asp Gly Ser Thr Ala 
1               5                   10                  15      


Asn Leu Val Pro Glu Val Asn Asn Glu Val Met Ala Leu Glu Pro Val 
            20                  25                  30          


Val Gly Ala Ala Ile Ala Ala Pro Val Ala Gly Gln Gln Asn Val Ile 
        35                  40                  45              


Asp Pro Trp Ile Arg Asn Asn Phe Cys Cys Ala Pro Gly Gly Glu Phe 
    50                  55                  60                  


Thr Val Ser Pro Ala Asn Ala Pro Gly Glu Ile Leu Trp Ser Ala Pro 
65                  70                  75                  80  


Leu Gly Pro Asp Leu Asn Pro Tyr Leu Ser His Leu Ala Arg Met Tyr 
                85                  90                  95      


Asn Gly Tyr Ala Gly Gly Phe Glu Val Gln Val Ile Leu Ala Gly Asn 
            100                 105                 110         


Ala Phe Thr Ala Gly Lys Val Ile Phe Ala Ala Val Pro Pro Asn Phe 
        115                 120                 125             


Pro Thr Glu Gly Leu Ser Pro Cys Gln Val Thr Met Phe Pro His Ile 
    130                 135                 140                 


Ile Val Asp Val Arg Gln Leu Glu Pro Val Leu Ile Pro Leu Pro Asp 
145                 150                 155                 160 


Val Arg Asn Asn Phe Tyr His Tyr Asn Gln Ser Asn Asp Ser Thr Ile 
                165                 170                 175     


Lys Leu Ile Ala Met Leu Tyr Thr Pro Leu Arg Ala Asn Asn Ala Gly 
            180                 185                 190         


Asp Asp Val Phe Thr Val Ser Cys Arg Val Leu Thr Arg Pro Ser Pro 
        195                 200                 205             


Asp Phe Asp Phe Ile Phe Leu Val Pro Pro Thr Val Glu His His His 
    210                 215                 220                 


His Leu Asp Gly Pro Tyr Gln Pro Ile Ala Phe Ser Pro Pro Pro Glu 
225                 230                 235                 240 


Tyr Tyr Ile Leu Leu Ser Pro Thr Ala Pro Gly Val Ile Ala Glu Cys 
                245                 250                 255     


Thr Asn Thr Val Asn Arg Trp Ile Ala Ile Ile Ala Ile Glu Pro Asn 
            260                 265                 270         


Val Ser Pro Thr Asn Arg Thr Tyr Thr Leu Phe Gly Ile Thr Glu Gln 
        275                 280                 285             


Leu Thr Val Glu Asn Ser Ser Val Asp Lys Trp Lys Phe Ile Asp Phe 
    290                 295                 300                 


Met Lys Thr Pro Thr Thr Gly Ser Tyr Val Arg Tyr Asn Ile Leu Leu 
305                 310                 315                 320 


Ser Ser Thr Lys Leu Cys Ala Val Ala Lys His Thr Asp Asn Leu Tyr 
                325                 330                 335     


Ser Tyr Val Gly Glu Thr Pro Thr Ala Gly Gln Ala Tyr Tyr Ser Ser 
            340                 345                 350         


Phe Asn Ile Phe Asn Leu Thr Ala His Cys Asp Phe Tyr Ile Ile Pro 
        355                 360                 365             


Trp Ser Gln Gln Ser Leu Cys Thr Gln Tyr Val Asn Asn Gly Leu 
    370                 375                 380             


<210>  42
<211>  498
<212>  DNA
<213>  Orthohepevirus Orthohepevirus A

<400>  42
cctaccccgt cacctgcccc ctcccgccct ttttcagttc ttcgtgccaa tgacgttctg       60

tggctctctc tcactgccgc tgagtacgac cagaccacgt atgggtcgtc caccaacccc      120

atgtatgtct ctgacacggt cacgtttgtt aatgtggcca ctggtgctca ggccgttgcc      180

cgctctcttg actggtctaa agtcaccctg gatggtcgtc ctcttaccac tattcagcag      240

tattctaaga cattttatgt tctcccgctt cgcgggaaac tttccttctg ggaggctagc      300

acgactaagg ccggctaccc gtataactat aatactactg ctagtgacca aattttgatt      360

gagaacgcgg ccggtcaccg tgtcgctatt tctacttata ccactagtct gggtgccggc      420

cctacctcga tctctgcggt cggtgtgcta gccccacatt cggcccttgc cgctcttgag      480

gacaccgttg attaccct                                                    498


<210>  43
<211>  166
<212>  PRT
<213>  Orthohepevirus Orthohepevirus A

<400>  43

Pro Thr Pro Ser Pro Ala Pro Ser Arg Pro Phe Ser Val Leu Arg Ala 
1               5                   10                  15      


Asn Asp Val Leu Trp Leu Ser Leu Thr Ala Ala Glu Tyr Asp Gln Thr 
            20                  25                  30          


Thr Tyr Gly Ser Ser Thr Asn Pro Met Tyr Val Ser Asp Thr Val Thr 
        35                  40                  45              


Phe Val Asn Val Ala Thr Gly Ala Gln Ala Val Ala Arg Ser Leu Asp 
    50                  55                  60                  


Trp Ser Lys Val Thr Leu Asp Gly Arg Pro Leu Thr Thr Ile Gln Gln 
65                  70                  75                  80  


Tyr Ser Lys Thr Phe Tyr Val Leu Pro Leu Arg Gly Lys Leu Ser Phe 
                85                  90                  95      


Trp Glu Ala Ser Thr Thr Lys Ala Gly Tyr Pro Tyr Asn Tyr Asn Thr 
            100                 105                 110         


Thr Ala Ser Asp Gln Ile Leu Ile Glu Asn Ala Ala Gly His Arg Val 
        115                 120                 125             


Ala Ile Ser Thr Tyr Thr Thr Ser Leu Gly Ala Gly Pro Thr Ser Ile 
    130                 135                 140                 


Ser Ala Val Gly Val Leu Ala Pro His Ser Ala Leu Ala Ala Leu Glu 
145                 150                 155                 160 


Asp Thr Val Asp Tyr Pro 
                165     


<210>  44
<211>  624
<212>  DNA
<213>  Avastrovirus Avastrovirus 2

<400>  44
tccatctatt tgccactacc acaagcagat gaccaataca caccctactt tgtctataat       60

tttcaagggg aaagggtgtc aaccaccgag actggggtat tttgtctggc agccatacca      120

gctgcgacta catctagtag gtataataat cagatcacca ctccatcaat tggctacagg      180

aatgctagtg gtacaggaac atcattccta ctagatgctg catcatggtg gaatatattg      240

gatgtaactc agactggagt gctttttgga caaccaagat tgggtgttgg tgtcatgcag      300

acaatgaaga ctctcaaaca gcatatcaag gattacacag agcctgcaat acagaaatat      360

tatcctggaa caactaacct tgatgagcag ttgaagcaga gattgaacct ggcagagggt      420

gacccggtca tctcaatggg ggacacaaac ggtaggaggg ctgcactctt ttataggact      480

agtgatgaaa aatatatttt atttttctca accacagaag atccaggggc acagtatcaa      540

aatctgaaaa tgttgtactt ctggaactgg tcctattctg acacaaaaca gcaatttttg      600

gaccacctta gaacagtgca gttt                                             624


<210>  45
<211>  208
<212>  PRT
<213>  Avastrovirus Avastrovirus 2

<400>  45

Ser Ile Tyr Leu Pro Leu Pro Gln Ala Asp Asp Gln Tyr Thr Pro Tyr 
1               5                   10                  15      


Phe Val Tyr Asn Phe Gln Gly Glu Arg Val Ser Thr Thr Glu Thr Gly 
            20                  25                  30          


Val Phe Cys Leu Ala Ala Ile Pro Ala Ala Thr Thr Ser Ser Arg Tyr 
        35                  40                  45              


Asn Asn Gln Ile Thr Thr Pro Ser Ile Gly Tyr Arg Asn Ala Ser Gly 
    50                  55                  60                  


Thr Gly Thr Ser Phe Leu Leu Asp Ala Ala Ser Trp Trp Asn Ile Leu 
65                  70                  75                  80  


Asp Val Thr Gln Thr Gly Val Leu Phe Gly Gln Pro Arg Leu Gly Val 
                85                  90                  95      


Gly Val Met Gln Thr Met Lys Thr Leu Lys Gln His Ile Lys Asp Tyr 
            100                 105                 110         


Thr Glu Pro Ala Ile Gln Lys Tyr Tyr Pro Gly Thr Thr Asn Leu Asp 
        115                 120                 125             


Glu Gln Leu Lys Gln Arg Leu Asn Leu Ala Glu Gly Asp Pro Val Ile 
    130                 135                 140                 


Ser Met Gly Asp Thr Asn Gly Arg Arg Ala Ala Leu Phe Tyr Arg Thr 
145                 150                 155                 160 


Ser Asp Glu Lys Tyr Ile Leu Phe Phe Ser Thr Thr Glu Asp Pro Gly 
                165                 170                 175     


Ala Gln Tyr Gln Asn Leu Lys Met Leu Tyr Phe Trp Asn Trp Ser Tyr 
            180                 185                 190         


Ser Asp Thr Lys Gln Gln Phe Leu Asp His Leu Arg Thr Val Gln Phe 
        195                 200                 205             


<210>  46
<211>  678
<212>  DNA
<213>  Influenzavirus A Influenza A virus

<400>  46
tgctctaaag gtaaacgtac cgttgacctg ggtcagtgcg gtctgctggg taccatcacc       60

ggtccgccgc agtgcgacca gttcctggaa ttctctgctg acctgatcat cgaacgtcgt      120

gaaggttctg acgtttgcta cccgggtaaa ttcgttaacg aagaagctct gcgtcagatc      180

ctgcgtgaat ctggtggtat cgacaaagaa accatgggtt tcacctacaa cggtatccgt      240

accaacggtg ttacctctgc ttgcaaacgt tctggttctt ctttctacgc tgaaatgaaa      300

tggctgctgt ctaacaccga caacgctgct ttcccgcaga tgaccaaatc ttacaaaaac      360

acccgtaaat ctccggctat catcgtttgg ggtatccacc actctgtttc taccgctgaa      420

cagaccaaac tgtacggttc tggtaacaaa ctggttaccg ttggttcttc taactaccag      480

cagtctttcg ttccgtctcc gggtgctcgt ccgcaggtta acggtctgtc tggtcgtatc      540

gacttccact ggctgatcct gaacccgaac gacaccgtta ccttctcttt caacggtgct      600

ttcatcgctc cggaccgtgc ttctttcctg cgtggtaaat ctatgggtat ccagtctggt      660

gttcaggttg acgctaac                                                    678


<210>  47
<211>  226
<212>  PRT
<213>  Influenzavirus A Influenza A virus

<400>  47

Cys Ser Lys Gly Lys Arg Thr Val Asp Leu Gly Gln Cys Gly Leu Leu 
1               5                   10                  15      


Gly Thr Ile Thr Gly Pro Pro Gln Cys Asp Gln Phe Leu Glu Phe Ser 
            20                  25                  30          


Ala Asp Leu Ile Ile Glu Arg Arg Glu Gly Ser Asp Val Cys Tyr Pro 
        35                  40                  45              


Gly Lys Phe Val Asn Glu Glu Ala Leu Arg Gln Ile Leu Arg Glu Ser 
    50                  55                  60                  


Gly Gly Ile Asp Lys Glu Thr Met Gly Phe Thr Tyr Asn Gly Ile Arg 
65                  70                  75                  80  


Thr Asn Gly Val Thr Ser Ala Cys Lys Arg Ser Gly Ser Ser Phe Tyr 
                85                  90                  95      


Ala Glu Met Lys Trp Leu Leu Ser Asn Thr Asp Asn Ala Ala Phe Pro 
            100                 105                 110         


Gln Met Thr Lys Ser Tyr Lys Asn Thr Arg Lys Ser Pro Ala Ile Ile 
        115                 120                 125             


Val Trp Gly Ile His His Ser Val Ser Thr Ala Glu Gln Thr Lys Leu 
    130                 135                 140                 


Tyr Gly Ser Gly Asn Lys Leu Val Thr Val Gly Ser Ser Asn Tyr Gln 
145                 150                 155                 160 


Gln Ser Phe Val Pro Ser Pro Gly Ala Arg Pro Gln Val Asn Gly Leu 
                165                 170                 175     


Ser Gly Arg Ile Asp Phe His Trp Leu Ile Leu Asn Pro Asn Asp Thr 
            180                 185                 190         


Val Thr Phe Ser Phe Asn Gly Ala Phe Ile Ala Pro Asp Arg Ala Ser 
        195                 200                 205             


Phe Leu Arg Gly Lys Ser Met Gly Ile Gln Ser Gly Val Gln Val Asp 
    210                 215                 220                 


Ala Asn 
225     


<210>  48
<211>  198
<212>  DNA
<213>  Plasmodium falciparum

<400>  48
gaaccgtctg acaaacacat caaagaatac ctgaacaaaa tccagaactc tctgtctacc       60

gaatggtctc cgtgctctgt tacctgcggt aacggtatcc aggttcgtat caaaccgggt      120

tctgctaaca aaccgaaaga cgaactggac tacgctaacg acatcgaaaa aaaaatctgc      180

aaaatggaaa aatgctct                                                    198


<210>  49
<211>  66
<212>  PRT
<213>  Plasmodium falciparum

<400>  49

Glu Pro Ser Asp Lys His Ile Lys Glu Tyr Leu Asn Lys Ile Gln Asn 
1               5                   10                  15      


Ser Leu Ser Thr Glu Trp Ser Pro Cys Ser Val Thr Cys Gly Asn Gly 
            20                  25                  30          


Ile Gln Val Arg Ile Lys Pro Gly Ser Ala Asn Lys Pro Lys Asp Glu 
        35                  40                  45              


Leu Asp Tyr Ala Asn Asp Ile Glu Lys Lys Ile Cys Lys Met Glu Lys 
    50                  55                  60                  


Cys Ser 
65      


<210>  50
<211>  69
<212>  DNA
<213>  Influenzavirus A influenza A

<400>  50
agtcttctaa ccgaggtcga aacgcctatc agaaacgaat gggggtgcag atgcaacgat       60

tcaagtgat                                                               69


<210>  51
<211>  23
<212>  PRT
<213>  Influenzavirus A influenza A

<400>  51

Ser Leu Leu Thr Glu Val Glu Thr Pro Ile Arg Asn Glu Trp Gly Cys 
1               5                   10                  15      


Arg Cys Asn Asp Ser Ser Asp 
            20              


