                         SEQUENCE LISTING

<110>  National University of Singapore
 
<120>  STIMULATORY CELL LINES FOR EX VIVO EXPANSION AND ACTIVATION OF 
       NATURAL KILLER CELLS

<130>  NKT.008WO

<150>  62/477311
<151>  2017-03-27

<160>  26    

<170>  PatentIn version 3.5

<210>  1
<211>  615
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Nucleic Acid sequence for mbIL15

<400>  1
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccgaactggg tgaatgtaat aagtgatttg aaaaaaattg aagatcttat tcaatctatg      120

catattgatg ctactttata tacggaaagt gatgttcacc ccagttgcaa agtaacagca      180

atgaagtgct ttctcttgga gttacaagtt atttcacttg agtccggaga tgcaagtatt      240

catgatacag tagaaaatct gatcatccta gcaaacaaca gtttgtcttc taatgggaat      300

gtaacagaat ctggatgcaa agaatgtgag gaactggagg aaaaaaatat taaagaattt      360

ttgcagagtt ttgtacatat tgtccaaatg ttcatcaaca cttctaccac gacgccagcg      420

ccgcgaccac caacaccggc gcccaccatc gcgtcgcagc ccctgtccct gcgcccagag      480

gcgtgccggc cagcggcggg gggcgcagtg cacacgaggg ggctggactt cgcctgtgat      540

atctacatct gggcgccctt ggccgggact tgtggggtcc ttctcctgtc actggttatc      600

accctttact gctaa                                                       615


<210>  2
<211>  204
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Amion Acid sequence for mbIL15

<400>  2

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys 
            20                  25                  30          


Ile Glu Asp Leu Ile Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr 
        35                  40                  45              


Glu Ser Asp Val His Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe 
    50                  55                  60                  


Leu Leu Glu Leu Gln Val Ile Ser Leu Glu Ser Gly Asp Ala Ser Ile 
65                  70                  75                  80  


His Asp Thr Val Glu Asn Leu Ile Ile Leu Ala Asn Asn Ser Leu Ser 
                85                  90                  95      


Ser Asn Gly Asn Val Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu 
            100                 105                 110         


Glu Glu Lys Asn Ile Lys Glu Phe Leu Gln Ser Phe Val His Ile Val 
        115                 120                 125             


Gln Met Phe Ile Asn Thr Ser Thr Thr Thr Pro Ala Pro Arg Pro Pro 
    130                 135                 140                 


Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu 
145                 150                 155                 160 


Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp 
                165                 170                 175     


Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly 
            180                 185                 190         


Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys 
        195                 200                 


<210>  3
<211>  867
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Nucleic Acid sequence for mbIL12A

<400>  3
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccgagaaacc tccccgtggc cactccagac ccaggaatgt tcccatgcct tcaccactcc      120

caaaacctgc tgagggccgt cagcaacatg ctccagaagg ccagacaaac tctagaattt      180

tacccttgca cttctgaaga gattgatcat gaagatatca caaaagataa aaccagcaca      240

gtggaggcct gtttaccatt ggaattaacc aagaatgaga gttgcctaaa ttccagagag      300

acctctttca taactaatgg gagttgcctg gcctccagaa agacctcttt tatgatggcc      360

ctgtgcctta gtagtattta tgaagacttg aagatgtacc aggtggagtt caagaccatg      420

aatgcaaagc ttctgatgga tcctaagagg cagatctttc tagatcaaaa catgctggca      480

gttattgatg agctgatgca ggccctgaat ttcaacagtg agactgtgcc acaaaaatcc      540

tcccttgaag aaccggattt ttataaaact aaaatcaagc tctgcatact tcttcatgct      600

ttcaggattc gggcagtgac tattgataga gtgatgagct atctgaatgc ttccaagccc      660

accacgacgc cagcgccgcg accaccaaca ccggcgccca ccatcgcgtc gcagcccctg      720

tccctgcgcc cagaggcgtg ccggccagcg gcggggggcg cagtgcacac gagggggctg      780

gacttcgcct gtgatatcta catctgggcg cccttggccg ggacttgtgg ggtccttctc      840

ctgtcactgg ttatcaccct ttactaa                                          867


<210>  4
<211>  288
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Amino Acid sequence for mbIL12A

<400>  4

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Arg Asn Leu Pro Val Ala Thr Pro Asp Pro Gly 
            20                  25                  30          


Met Phe Pro Cys Leu His His Ser Gln Asn Leu Leu Arg Ala Val Ser 
        35                  40                  45              


Asn Met Leu Gln Lys Ala Arg Gln Thr Leu Glu Phe Tyr Pro Cys Thr 
    50                  55                  60                  


Ser Glu Glu Ile Asp His Glu Asp Ile Thr Lys Asp Lys Thr Ser Thr 
65                  70                  75                  80  


Val Glu Ala Cys Leu Pro Leu Glu Leu Thr Lys Asn Glu Ser Cys Leu 
                85                  90                  95      


Asn Ser Arg Glu Thr Ser Phe Ile Thr Asn Gly Ser Cys Leu Ala Ser 
            100                 105                 110         


Arg Lys Thr Ser Phe Met Met Ala Leu Cys Leu Ser Ser Ile Tyr Glu 
        115                 120                 125             


Asp Leu Lys Met Tyr Gln Val Glu Phe Lys Thr Met Asn Ala Lys Leu 
    130                 135                 140                 


Leu Met Asp Pro Lys Arg Gln Ile Phe Leu Asp Gln Asn Met Leu Ala 
145                 150                 155                 160 


Val Ile Asp Glu Leu Met Gln Ala Leu Asn Phe Asn Ser Glu Thr Val 
                165                 170                 175     


Pro Gln Lys Ser Ser Leu Glu Glu Pro Asp Phe Tyr Lys Thr Lys Ile 
            180                 185                 190         


Lys Leu Cys Ile Leu Leu His Ala Phe Arg Ile Arg Ala Val Thr Ile 
        195                 200                 205             


Asp Arg Val Met Ser Tyr Leu Asn Ala Ser Lys Pro Thr Thr Thr Pro 
    210                 215                 220                 


Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu 
225                 230                 235                 240 


Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His 
                245                 250                 255     


Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu 
            260                 265                 270         


Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr 
        275                 280                 285             


<210>  5
<211>  1194
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Nucleic Acid sequence for mbIL12B

<400>  5
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccgatatggg aactgaagaa agatgtttat gtcgtagaat tggattggta tccggatgcc      120

cctggagaaa tggtggtcct cacctgtgac acccctgaag aagatggtat cacctggacc      180

ttggaccaga gcagtgaggt cttaggctct ggcaaaaccc tgaccatcca agtcaaagag      240

tttggagatg ctggccagta cacctgtcac aaaggaggcg aggttctaag ccattcgctc      300

ctgctgcttc acaaaaagga agatggaatt tggtccactg atattttaaa ggaccagaaa      360

gaacccaaaa ataagacctt tctaagatgc gaggccaaga attattctgg acgtttcacc      420

tgctggtggc tgacgacaat cagtactgat ttgacattca gtgtcaaaag cagcagaggc      480

tcttctgacc cccaaggggt gacgtgcgga gctgctacac tctctgcaga gagagtcaga      540

ggggacaaca aggagtatga gtactcagtg gagtgccagg aggacagtgc ctgcccagct      600

gctgaggaga gtctgcccat tgaggtcatg gtggatgccg ttcacaagct caagtatgaa      660

aactacacca gcagcttctt catcagggac atcatcaaac ctgacccacc caagaacttg      720

cagctgaagc cattaaagaa ctctcggcag gtggaggtca gctgggagta ccctgacacc      780

tggagtactc cacattccta cttctccctg acattctgcg ttcaggtcca gggcaagagc      840

aagagagaaa agaaagatag agtcttcacg gacaagacct cagccacggt catctgccgc      900

aaaaatgcca gcattagcgt gcgggcccag gaccgctact atagctcatc ttggagcgaa      960

tgggcatctg tgccctgcag taagcccacc acgacgccag cgccgcgacc accaacaccg     1020

gcgcccacca tcgcgtcgca gcccctgtcc ctgcgcccag aggcgtgccg gccagcggcg     1080

gggggcgcag tgcacacgag ggggctggac ttcgcctgtg atatctacat ctgggcgccc     1140

ttggccggga cttgtggggt ccttctcctg tcactggtta tcacccttta ctaa           1194


<210>  6
<211>  397
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Amino Acid sequence for mbIL12B

<400>  6

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Ile Trp Glu Leu Lys Lys Asp Val Tyr Val Val 
            20                  25                  30          


Glu Leu Asp Trp Tyr Pro Asp Ala Pro Gly Glu Met Val Val Leu Thr 
        35                  40                  45              


Cys Asp Thr Pro Glu Glu Asp Gly Ile Thr Trp Thr Leu Asp Gln Ser 
    50                  55                  60                  


Ser Glu Val Leu Gly Ser Gly Lys Thr Leu Thr Ile Gln Val Lys Glu 
65                  70                  75                  80  


Phe Gly Asp Ala Gly Gln Tyr Thr Cys His Lys Gly Gly Glu Val Leu 
                85                  90                  95      


Ser His Ser Leu Leu Leu Leu His Lys Lys Glu Asp Gly Ile Trp Ser 
            100                 105                 110         


Thr Asp Ile Leu Lys Asp Gln Lys Glu Pro Lys Asn Lys Thr Phe Leu 
        115                 120                 125             


Arg Cys Glu Ala Lys Asn Tyr Ser Gly Arg Phe Thr Cys Trp Trp Leu 
    130                 135                 140                 


Thr Thr Ile Ser Thr Asp Leu Thr Phe Ser Val Lys Ser Ser Arg Gly 
145                 150                 155                 160 


Ser Ser Asp Pro Gln Gly Val Thr Cys Gly Ala Ala Thr Leu Ser Ala 
                165                 170                 175     


Glu Arg Val Arg Gly Asp Asn Lys Glu Tyr Glu Tyr Ser Val Glu Cys 
            180                 185                 190         


Gln Glu Asp Ser Ala Cys Pro Ala Ala Glu Glu Ser Leu Pro Ile Glu 
        195                 200                 205             


Val Met Val Asp Ala Val His Lys Leu Lys Tyr Glu Asn Tyr Thr Ser 
    210                 215                 220                 


Ser Phe Phe Ile Arg Asp Ile Ile Lys Pro Asp Pro Pro Lys Asn Leu 
225                 230                 235                 240 


Gln Leu Lys Pro Leu Lys Asn Ser Arg Gln Val Glu Val Ser Trp Glu 
                245                 250                 255     


Tyr Pro Asp Thr Trp Ser Thr Pro His Ser Tyr Phe Ser Leu Thr Phe 
            260                 265                 270         


Cys Val Gln Val Gln Gly Lys Ser Lys Arg Glu Lys Lys Asp Arg Val 
        275                 280                 285             


Phe Thr Asp Lys Thr Ser Ala Thr Val Ile Cys Arg Lys Asn Ala Ser 
    290                 295                 300                 


Ile Ser Val Arg Ala Gln Asp Arg Tyr Tyr Ser Ser Ser Trp Ser Glu 
305                 310                 315                 320 


Trp Ala Ser Val Pro Cys Ser Lys Pro Thr Thr Thr Pro Ala Pro Arg 
                325                 330                 335     


Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg 
            340                 345                 350         


Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly 
        355                 360                 365             


Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr 
    370                 375                 380                 


Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr 
385                 390                 395         


<210>  7
<211>  747
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Nucleic Acid sequence for mbIL18

<400>  7
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccgtactttg gcaagcttga atctaaatta tcagtcataa gaaatttgaa tgaccaagtt      120

ctcttcattg accaaggaaa tcggcctcta tttgaagata tgactgattc tgactgtaga      180

gataatgcac cccggaccat atttattata agtatgtata aagatagcca gcctagaggt      240

atggctgtaa ctatctctgt gaagtgtgag aaaatttcaa ctctctcctg tgagaacaaa      300

attatttcct ttaaggaaat gaatcctcct gataacatca aggatacaaa aagtgacatc      360

atattctttc agagaagtgt cccaggacat gataataaga tgcaatttga atcttcatca      420

tacgaaggat actttctagc ttgtgaaaaa gagagagacc tttttaaact cattttgaaa      480

aaagaggatg aattggggga tagatctata atgttcactg ttcaaaacga agacaagccc      540

accacgacgc cagcgccgcg accaccaaca ccggcgccca ccatcgcgtc gcagcccctg      600

tccctgcgcc cagaggcgtg ccggccagcg gcggggggcg cagtgcacac gagggggctg      660

gacttcgcct gtgatatcta catctgggcg cccttggccg ggacttgtgg ggtccttctc      720

ctgtcactgg ttatcaccct ttactaa                                          747


<210>  8
<211>  248
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Amino Acid sequence for mbIL18

<400>  8

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Tyr Phe Gly Lys Leu Glu Ser Lys Leu Ser Val 
            20                  25                  30          


Ile Arg Asn Leu Asn Asp Gln Val Leu Phe Ile Asp Gln Gly Asn Arg 
        35                  40                  45              


Pro Leu Phe Glu Asp Met Thr Asp Ser Asp Cys Arg Asp Asn Ala Pro 
    50                  55                  60                  


Arg Thr Ile Phe Ile Ile Ser Met Tyr Lys Asp Ser Gln Pro Arg Gly 
65                  70                  75                  80  


Met Ala Val Thr Ile Ser Val Lys Cys Glu Lys Ile Ser Thr Leu Ser 
                85                  90                  95      


Cys Glu Asn Lys Ile Ile Ser Phe Lys Glu Met Asn Pro Pro Asp Asn 
            100                 105                 110         


Ile Lys Asp Thr Lys Ser Asp Ile Ile Phe Phe Gln Arg Ser Val Pro 
        115                 120                 125             


Gly His Asp Asn Lys Met Gln Phe Glu Ser Ser Ser Tyr Glu Gly Tyr 
    130                 135                 140                 


Phe Leu Ala Cys Glu Lys Glu Arg Asp Leu Phe Lys Leu Ile Leu Lys 
145                 150                 155                 160 


Lys Glu Asp Glu Leu Gly Asp Arg Ser Ile Met Phe Thr Val Gln Asn 
                165                 170                 175     


Glu Asp Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala 
            180                 185                 190         


Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg 
        195                 200                 205             


Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys 
    210                 215                 220                 


Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu 
225                 230                 235                 240 


Leu Ser Leu Val Ile Thr Leu Tyr 
                245             


<210>  9
<211>  706
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Nucleic Acid sequence for mbIL21

<220>
<221>  misc_feature
<223>  Nucleic Acid sequence for IL21

<400>  9
gctgaagtga aaacgagacc aaggtctagc tctactgttg gtacttatga gatccagtcc       60

tggcaacatg gagaggattg tcatctgtct gatggtcatc tcttggggac actggtccac      120

aaatcaagct cccaaggtca agatcgccac atgattagaa tgcgtcaact tatagatatt      180

gttgatcagc tgaaaaatta tgtgaatgac ttggtccctg aatttctgcc agctccagaa      240

gatgtagaga caaactgtga gtggtcagct ttttcctgct ttcagaaggc ccaactaaag      300

tcagcaaata caggaaacaa tgaaaggata atcaatgtat caattaaaaa gctgaagagg      360

aaaccacctt ccacaaatgc agggagaaga cagaaacaca gactaacatg cccttcatgt      420

gattcttatg agaaaaaacc acccaaagaa ttcctagaaa gattcaaatc acttctccaa      480

aaggtatcta ccttaagttt catttgattt tctgctttat ctttacctat ccagatttgc      540

ttcttagtta ctcacggtat actatttcca cagatgattc atcagcatct gtcctctaga      600

acacacggaa gtgaagattc ctgaggatct aacttgcagt tggacactat gttacatact      660

ctaatatagt agtgaaagtc atttctttgt attccaagtg gaggag                     706


<210>  10
<211>  155
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Amino Acid sequence for mbIL21

<220>
<221>  MISC_FEATURE
<223>  Amino Acid sequence for IL21

<400>  10

Met Glu Arg Ile Val Ile Cys Leu Met Val Ile Phe Leu Gly Thr Leu 
1               5                   10                  15      


Val His Lys Ser Ser Ser Gln Gly Gln Asp Arg His Met Ile Arg Met 
            20                  25                  30          


Arg Gln Leu Ile Asp Ile Val Asp Gln Leu Lys Asn Tyr Val Asn Asp 
        35                  40                  45              


Leu Val Pro Glu Phe Leu Pro Ala Pro Glu Asp Val Glu Thr Asn Cys 
    50                  55                  60                  


Glu Trp Ser Ala Phe Ser Cys Phe Gln Lys Ala Gln Leu Lys Ser Ala 
65                  70                  75                  80  


Asn Thr Gly Asn Asn Glu Arg Ile Ile Asn Val Ser Ile Lys Lys Leu 
                85                  90                  95      


Lys Arg Lys Pro Pro Ser Thr Asn Ala Gly Arg Arg Gln Lys His Arg 
            100                 105                 110         


Leu Thr Cys Pro Ser Cys Asp Ser Tyr Glu Lys Lys Pro Pro Lys Glu 
        115                 120                 125             


Phe Leu Glu Arg Phe Lys Ser Leu Leu Gln Lys Met Ile His Gln His 
    130                 135                 140                 


Leu Ser Ser Arg Thr His Gly Ser Glu Asp Ser 
145                 150                 155 


<210>  11
<211>  1147
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Nucleic Acid sequence for IL22

<400>  11
cgaccaggtt ctccttcccc agtcaccagt tgctcgagtt agaattgtct gcaatggccg       60

ccctgcagaa atctgtgagc tctttcctta tggggaccct ggccaccagc tgcctccttc      120

tcttggccct cttggtacag ggaggagcag ctgcgcccat cagctcccac tgcaggcttg      180

acaagtccaa cttccagcag ccctatatca ccaaccgcac cttcatgctg gctaaggagg      240

ctagcttggc tgataacaac acagacgttc gtctcattgg ggagaaactg ttccacggag      300

tcagtatgag tgagcgctgc tatctgatga agcaggtgct gaacttcacc cttgaagaag      360

tgctgttccc tcaatctgat aggttccagc cttatatgca ggaggtggtg cccttcctgg      420

ccaggctcag caacaggcta agcacatgtc atattgaagg tgatgacctg catatccaga      480

ggaatgtgca aaagctgaag gacacagtga aaaagcttgg agagagtgga gagatcaaag      540

caattggaga actggatttg ctgtttatgt ctctgagaaa tgcctgcatt tgaccagagc      600

aaagctgaaa aatgaataac taaccccctt tccctgctag aaataacaat tagatgcccc      660

aaagcgattt tttttaacca aaaggaagat gggaagccaa actccatcat gatgggtgga      720

ttccaaatga acccctgcgt tagttacaaa ggaaaccaat gccacttttg tttataagac      780

cagaaggtag actttctaag catagatatt tattgataac atttcattgt aactggtgtt      840

ctatacacag aaaacaattt attttttaaa taattgtctt tttccataaa aaagattact      900

ttccattcct ttaggggaaa aaacccctaa atagcttcat gtttccataa tcagtacttt      960

atatttataa atgtatttat tattattata agactgcatt ttatttatat cattttatta     1020

atatggattt atttatagaa acatcattcg atattgctac ttgagtgtaa ggctaatatt     1080

gatatttatg acaataatta tagagctata acatgtttat ttgacctcaa taaacacttg     1140

gatatcc                                                               1147


<210>  12
<211>  179
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Amino acid sequence for IL22

<400>  12

Met Ala Ala Leu Gln Lys Ser Val Ser Ser Phe Leu Met Gly Thr Leu 
1               5                   10                  15      


Ala Thr Ser Cys Leu Leu Leu Leu Ala Leu Leu Val Gln Gly Gly Ala 
            20                  25                  30          


Ala Ala Pro Ile Ser Ser His Cys Arg Leu Asp Lys Ser Asn Phe Gln 
        35                  40                  45              


Gln Pro Tyr Ile Thr Asn Arg Thr Phe Met Leu Ala Lys Glu Ala Ser 
    50                  55                  60                  


Leu Ala Asp Asn Asn Thr Asp Val Arg Leu Ile Gly Glu Lys Leu Phe 
65                  70                  75                  80  


His Gly Val Ser Met Ser Glu Arg Cys Tyr Leu Met Lys Gln Val Leu 
                85                  90                  95      


Asn Phe Thr Leu Glu Glu Val Leu Phe Pro Gln Ser Asp Arg Phe Gln 
            100                 105                 110         


Pro Tyr Met Gln Glu Val Val Pro Phe Leu Ala Arg Leu Ser Asn Arg 
        115                 120                 125             


Leu Ser Thr Cys His Ile Glu Gly Asp Asp Leu His Ile Gln Arg Asn 
    130                 135                 140                 


Val Gln Lys Leu Lys Asp Thr Val Lys Lys Leu Gly Glu Ser Gly Glu 
145                 150                 155                 160 


Ile Lys Ala Ile Gly Glu Leu Asp Leu Leu Phe Met Ser Leu Arg Asn 
                165                 170                 175     


Ala Cys Ile 
            


<210>  13
<211>  765
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Nucleic Acid sequence for 4-1BB ligand

<400>  13
atggaatacg cctctgacgc ttcactggac cccgaagccc cgtggcctcc cgcgccccgc       60

gctcgcgcct gccgcgtact gccttgggcc ctggtcgcgg ggctgctgct gctgctgctg      120

ctcgctgccg cctgcgccgt cttcctcgcc tgcccctggg ccgtgtccgg ggctcgcgcc      180

tcgcccggct ccgcggccag cccgagactc cgcgagggtc ccgagctttc gcccgacgat      240

cccgccggcc tcttggacct gcggcagggc atgtttgcgc agctggtggc ccaaaatgtt      300

ctgctgatcg atgggcccct gagctggtac agtgacccag gcctggcagg cgtgtccctg      360

acggggggcc tgagctacaa agaggacacg aaggagctgg tggtggccaa ggctggagtc      420

tactatgtct tctttcaact agagctgcgg cgcgtggtgg ccggcgaggg ctcaggctcc      480

gtttcacttg cgctgcacct gcagccactg cgctctgctg ctggggccgc cgccctggct      540

ttgaccgtgg acctgccacc cgcctcctcc gaggctcgga actcggcctt cggtttccag      600

ggccgcttgc tgcacctgag tgccggccag cgcctgggcg tccatcttca cactgaggcc      660

agggcacgcc atgcctggca gcttacccag ggcgccacag tcttgggact cttccgggtg      720

acccccgaaa tcccagccgg actcccttca ccgaggtcgg aataa                      765


<210>  14
<211>  254
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Amino Acid sequence for 4-1BB ligand

<400>  14

Met Glu Tyr Ala Ser Asp Ala Ser Leu Asp Pro Glu Ala Pro Trp Pro 
1               5                   10                  15      


Pro Ala Pro Arg Ala Arg Ala Cys Arg Val Leu Pro Trp Ala Leu Val 
            20                  25                  30          


Ala Gly Leu Leu Leu Leu Leu Leu Leu Ala Ala Ala Cys Ala Val Phe 
        35                  40                  45              


Leu Ala Cys Pro Trp Ala Val Ser Gly Ala Arg Ala Ser Pro Gly Ser 
    50                  55                  60                  


Ala Ala Ser Pro Arg Leu Arg Glu Gly Pro Glu Leu Ser Pro Asp Asp 
65                  70                  75                  80  


Pro Ala Gly Leu Leu Asp Leu Arg Gln Gly Met Phe Ala Gln Leu Val 
                85                  90                  95      


Ala Gln Asn Val Leu Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp 
            100                 105                 110         


Pro Gly Leu Ala Gly Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu 
        115                 120                 125             


Asp Thr Lys Glu Leu Val Val Ala Lys Ala Gly Val Tyr Tyr Val Phe 
    130                 135                 140                 


Phe Gln Leu Glu Leu Arg Arg Val Val Ala Gly Glu Gly Ser Gly Ser 
145                 150                 155                 160 


Val Ser Leu Ala Leu His Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala 
                165                 170                 175     


Ala Ala Leu Ala Leu Thr Val Asp Leu Pro Pro Ala Ser Ser Glu Ala 
            180                 185                 190         


Arg Asn Ser Ala Phe Gly Phe Gln Gly Arg Leu Leu His Leu Ser Ala 
        195                 200                 205             


Gly Gln Arg Leu Gly Val His Leu His Thr Glu Ala Arg Ala Arg His 
    210                 215                 220                 


Ala Trp Gln Leu Thr Gln Gly Ala Thr Val Leu Gly Leu Phe Arg Val 
225                 230                 235                 240 


Thr Pro Glu Ile Pro Ala Gly Leu Pro Ser Pro Arg Ser Glu 
                245                 250                 


<210>  15
<211>  1534
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Nucleic Acid sequence for CD3 Receptor epsilon subunit

<400>  15
tattgtcaga gtcctcttgt ttggccttct aggaaggctg tgggacccag ctttcttcaa       60

ccagtccagg tggaggcctc tgccttgaac gtttccaagt gaggtaaaac ccgcaggccc      120

agaggcctct ctacttcctg tgtggggttc agaaaccctc ctcccctccc agcctcaggt      180

gcctgcttca gaaaatgaag tagtaagtct gctggcctcc gccatcttag taaagtaaca      240

gtcccatgaa acaaagatgc agtcgggcac tcactggaga gttctgggcc tctgcctctt      300

atcagttggc gtttgggggc aagatggtaa tgaagaaatg ggtggtatta cacagacacc      360

atataaagtc tccatctctg gaaccacagt aatattgaca tgccctcagt atcctggatc      420

tgaaatacta tggcaacaca atgataaaaa cataggcggt gatgaggatg ataaaaacat      480

aggcagtgat gaggatcacc tgtcactgaa ggaattttca gaattggagc aaagtggtta      540

ttatgtctgc taccccagag gaagcaaacc agaagatgcg aacttttatc tctacctgag      600

ggcaagagtg tgtgagaact gcatggagat ggatgtgatg tcggtggcca caattgtcat      660

agtggacatc tgcatcactg ggggcttgct gctgctggtt tactactgga gcaagaatag      720

aaaggccaag gccaagcctg tgacacgagg agcgggtgct ggcggcaggc aaaggggaca      780

aaacaaggag aggccaccac ctgttcccaa cccagactat gagcccatcc ggaaaggcca      840

gcgggacctg tattctggcc tgaatcagag acgcatctga ccctctggag aacactgcct      900

cccgctggcc caggtctcct ctccagtccc cctgcgactc cctgtttcct gggctagtct      960

tggaccccac gagagagaat cgttcctcag cctcatggtg aactcgcgcc ctccagcctg     1020

atcccccgct ccctcctccc tgccttctct gctggtaccc agtcctaaaa tattgctgct     1080

tcctcttcct ttgaagcatc atcagtagtc acaccctcac agctggcctg ccctcttgcc     1140

aggatattta tttgtgctat tcactccctt ccctttggat gtaacttctc cgttcagttc     1200

cctccttttc ttgcatgtaa gttgtccccc atcccaaagt attccatcta cttttctatc     1260

gccgtcccct tttgcagccc tctctgggga tggactgggt aaatgttgac agaggccctg     1320

ccccgttcac agatcctggc cctgagccag ccctgtgctc ctccctcccc caacactccc     1380

taccaacccc ctaatcccct actccctcca ccccccctcc actgtaggcc actggatggt     1440

catttgcatc tccgtaaatg tgctctgctc ctcagctgag agagaaaaaa ataaactgta     1500

tttggctgca agaaaaaaaa aaaaaaaaaa aaaa                                 1534


<210>  16
<211>  1017
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Nucleic Acid sequence for mb-anti-CD3 scFv

<400>  16
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccgcaaattg ttctcaccca gtctccagca atcatgtctg catctccagg ggagaaggtc      120

accatgacct gcagtgccag ctcaagtgta agttacatga actggtacca gcagaagtca      180

ggcacctccc ccaaaagatg gatttatgac acatccaaac tggcttctgg agtccctgct      240

cacttcaggg gcagtgggtc tgggacctct tactctctca caatcagcgg catggaggct      300

gaagatgctg ccacttatta ctgccagcag tggagtagta acccattcac gttcggctcg      360

gggacaaagt tggaaataaa ccggggtggt ggtggttctg gtggtggtgg ttctggcggc      420

ggcggctccg gtggtggtgg atccgaggtc cagctgcagc agtctggggc tgaactggca      480

agacctgggg cctcagtgaa gatgtcctgc aaggcttctg gctacacctt tactaggtac      540

acgatgcact gggtaaaaca gaggcctgga cagggtctgg aatggattgg atacattaat      600

cctagccgtg gttatactaa ttacaatcag aagttcaagg acaaggccac attgactaca      660

gacaaatcct ccagcacagc ctacatgcaa ctgagcagcc tgacatctga ggactctgca      720

gtctattact gtgcaagata ttatgatgat cattactgcc ttgactactg gggccaaggc      780

accactctca cagtctcctc agccaagccc accacgacgc cagcgccgcg accaccaaca      840

ccggcgccca ccatcgcgtc gcagcccctg tccctgcgcc cagaggcgtg ccggccagcg      900

gcggggggcg cagtgcacac gagggggctg gacttcgcct gtgatatcta catctgggcg      960

cccttggccg ggacttgtgg ggtccttctc ctgtcactgg ttatcaccct ttactaa        1017


<210>  17
<211>  338
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Amino Acid sequence for mb-anti-CD3 scFv

<400>  17

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gln Ile Val Leu Thr Gln Ser Pro Ala Ile Met 
            20                  25                  30          


Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Ser Ala Ser Ser 
        35                  40                  45              


Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln Lys Ser Gly Thr Ser Pro 
    50                  55                  60                  


Lys Arg Trp Ile Tyr Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ala 
65                  70                  75                  80  


His Phe Arg Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser 
                85                  90                  95      


Gly Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser 
            100                 105                 110         


Ser Asn Pro Phe Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Asn Arg 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Glu Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala 
145                 150                 155                 160 


Arg Pro Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr 
                165                 170                 175     


Phe Thr Arg Tyr Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly 
            180                 185                 190         


Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr 
        195                 200                 205             


Asn Gln Lys Phe Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser 
    210                 215                 220                 


Ser Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala 
225                 230                 235                 240 


Val Tyr Tyr Cys Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr 
                245                 250                 255     


Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Ala Lys Pro Thr Thr 
            260                 265                 270         


Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln 
        275                 280                 285             


Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala 
    290                 295                 300                 


Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala 
305                 310                 315                 320 


Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr 
                325                 330                 335     


Leu Tyr 
        


<210>  18
<211>  45
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Amino Acid sequence for CD8 alpha transmembrane domain

<400>  18

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        35                  40                  45  


<210>  19
<211>  8
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Amino Acid sequence for FLAG tag

<400>  19

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  20
<211>  6
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Amino Acid sequence for His tag

<400>  20

His His His His His His 
1               5       


<210>  21
<211>  638
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Nucleic acid cloning construct for mbIL15

<400>  21
gaattcgccc ttccaccatg gccttaccag tgaccgcctt gctcctgccg ctggccttgc       60

tgctccacgc cgccaggccg aactgggtga atgtaataag tgatttgaaa aaaattgaag      120

atcttattca atctatgcat attgatgcta ctttatatac ggaaagtgat gttcacccca      180

gttgcaaagt aacagcaatg aagtgctttc tcttggagtt acaagttatt tcacttgagt      240

ccggagatgc aagtattcat gatacagtag aaaatctgat catcctagca aacaacagtt      300

tgtcttctaa tgggaatgta acagaatctg gatgcaaaga atgtgaggaa ctggaggaaa      360

aaaatattaa agaatttttg cagagttttg tacatattgt ccaaatgttc atcaacactt      420

ctaccacgac gccagcgccg cgaccaccaa caccggcgcc caccatcgcg tcgcagcccc      480

tgtccctgcg cccagaggcg tgccggccag cggcgggggg cgcagtgcac acgagggggc      540

tggacttcgc ctgtgatatc tacatctggg cgcccttggc cgggacttgt ggggtccttc      600

tcctgtcact ggttatcacc ctttactgct aactcgag                              638


<210>  22
<211>  788
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Nucleic acid cloning construct for 4-1BB ligand

<400>  22
gaattcgccc ttccaccatg gaatacgcct ctgacgcttc actggacccc gaagccccgt       60

ggcctcccgc gccccgcgct cgcgcctgcc gcgtactgcc ttgggccctg gtcgcggggc      120

tgctgctgct gctgctgctc gctgccgcct gcgccgtctt cctcgcctgc ccctgggccg      180

tgtccggggc tcgcgcctcg cccggctccg cggccagccc gagactccgc gagggtcccg      240

agctttcgcc cgacgatccc gccggcctct tggacctgcg gcagggcatg tttgcgcagc      300

tggtggccca aaatgttctg ctgatcgatg ggcccctgag ctggtacagt gacccaggcc      360

tggcaggcgt gtccctgacg gggggcctga gctacaaaga ggacacgaag gagctggtgg      420

tggccaaggc tggagtctac tatgtcttct ttcaactaga gctgcggcgc gtggtggccg      480

gcgagggctc aggctccgtt tcacttgcgc tgcacctgca gccactgcgc tctgctgctg      540

gggccgccgc cctggctttg accgtggacc tgccacccgc ctcctccgag gctcggaact      600

cggccttcgg tttccagggc cgcttgctgc acctgagtgc cggccagcgc ctgggcgtcc      660

atcttcacac tgaggccagg gcacgccatg cctggcagct tacccagggc gccacagtct      720

tgggactctt ccgggtgacc cccgaaatcc cagccggact cccttcaccg aggtcggaat      780

aactcgag                                                               788


<210>  23
<211>  889
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Nucleic acid cloning construct for mbIL12A

<400>  23
gaattcggct tccaccatgg ccttaccagt gaccgccttg ctcctgccgc tggccttgct       60

gctccacgcc gccaggccga gaaacctccc cgtggccact ccagacccag gaatgttccc      120

atgccttcac cactcccaaa acctgctgag ggccgtcagc aacatgctcc agaaggccag      180

acaaactcta gaattttacc cttgcacttc tgaagagatt gatcatgaag atatcacaaa      240

agataaaacc agcacagtgg aggcctgttt accattggaa ttaaccaaga atgagagttg      300

cctaaattcc agagagacct ctttcataac taatgggagt tgcctggcct ccagaaagac      360

ctcttttatg atggccctgt gccttagtag tatttatgaa gacttgaaga tgtaccaggt      420

ggagttcaag accatgaatg caaagcttct gatggatcct aagaggcaga tctttctaga      480

tcaaaacatg ctggcagtta ttgatgagct gatgcaggcc ctgaatttca acagtgagac      540

tgtgccacaa aaatcctccc ttgaagaacc ggatttttat aaaactaaaa tcaagctctg      600

catacttctt catgctttca ggattcgggc agtgactatt gatagagtga tgagctatct      660

gaatgcttcc aagcccacca cgacgccagc gccgcgacca ccaacaccgg cgcccaccat      720

cgcgtcgcag cccctgtccc tgcgcccaga ggcgtgccgg ccagcggcgg ggggcgcagt      780

gcacacgagg gggctggact tcgcctgtga tatctacatc tgggcgccct tggccgggac      840

ttgtggggtc cttctcctgt cactggttat caccctttac taactcgag                  889


<210>  24
<211>  1216
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Nucleic acid cloning construct for mbIL12B

<400>  24
gaattcggct tccaccatgg ccttaccagt gaccgccttg ctcctgccgc tggccttgct       60

gctccacgcc gccaggccga tatgggaact gaagaaagat gtttatgtcg tagaattgga      120

ttggtatccg gatgcccctg gagaaatggt ggtcctcacc tgtgacaccc ctgaagaaga      180

tggtatcacc tggaccttgg accagagcag tgaggtctta ggctctggca aaaccctgac      240

catccaagtc aaagagtttg gagatgctgg ccagtacacc tgtcacaaag gaggcgaggt      300

tctaagccat tcgctcctgc tgcttcacaa aaaggaagat ggaatttggt ccactgatat      360

tttaaaggac cagaaagaac ccaaaaataa gacctttcta agatgcgagg ccaagaatta      420

ttctggacgt ttcacctgct ggtggctgac gacaatcagt actgatttga cattcagtgt      480

caaaagcagc agaggctctt ctgaccccca aggggtgacg tgcggagctg ctacactctc      540

tgcagagaga gtcagagggg acaacaagga gtatgagtac tcagtggagt gccaggagga      600

cagtgcctgc ccagctgctg aggagagtct gcccattgag gtcatggtgg atgccgttca      660

caagctcaag tatgaaaact acaccagcag cttcttcatc agggacatca tcaaacctga      720

cccacccaag aacttgcagc tgaagccatt aaagaactct cggcaggtgg aggtcagctg      780

ggagtaccct gacacctgga gtactccaca ttcctacttc tccctgacat tctgcgttca      840

ggtccagggc aagagcaaga gagaaaagaa agatagagtc ttcacggaca agacctcagc      900

cacggtcatc tgccgcaaaa atgccagcat tagcgtgcgg gcccaggacc gctactatag      960

ctcatcttgg agcgaatggg catctgtgcc ctgcagtaag cccaccacga cgccagcgcc     1020

gcgaccacca acaccggcgc ccaccatcgc gtcgcagccc ctgtccctgc gcccagaggc     1080

gtgccggcca gcggcggggg gcgcagtgca cacgaggggg ctggacttcg cctgtgatat     1140

ctacatctgg gcgcccttgg ccgggacttg tggggtcctt ctcctgtcac tggttatcac     1200

cctttactaa ctcgag                                                     1216


<210>  25
<211>  769
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Nucleic acid cloning construct for mbIL18

<400>  25
gaattcggct tccaccatgg ccttaccagt gaccgccttg ctcctgccgc tggccttgct       60

gctccacgcc gccaggccgt actttggcaa gcttgaatct aaattatcag tcataagaaa      120

tttgaatgac caagttctct tcattgacca aggaaatcgg cctctatttg aagatatgac      180

tgattctgac tgtagagata atgcaccccg gaccatattt attataagta tgtataaaga      240

tagccagcct agaggtatgg ctgtaactat ctctgtgaag tgtgagaaaa tttcaactct      300

ctcctgtgag aacaaaatta tttcctttaa ggaaatgaat cctcctgata acatcaagga      360

tacaaaaagt gacatcatat tctttcagag aagtgtccca ggacatgata ataagatgca      420

atttgaatct tcatcatacg aaggatactt tctagcttgt gaaaaagaga gagacctttt      480

taaactcatt ttgaaaaaag aggatgaatt gggggataga tctataatgt tcactgttca      540

aaacgaagac aagcccacca cgacgccagc gccgcgacca ccaacaccgg cgcccaccat      600

cgcgtcgcag cccctgtccc tgcgcccaga ggcgtgccgg ccagcggcgg ggggcgcagt      660

gcacacgagg gggctggact tcgcctgtga tatctacatc tgggcgccct tggccgggac      720

ttgtggggtc cttctcctgt cactggttat caccctttac taactcgag                  769


<210>  26
<211>  1039
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Nucleic acid cloning construct for mb-anti CD3 scFv

<400>  26
gaattcggct tccaccatgg ccttaccagt gaccgccttg ctcctgccgc tggccttgct       60

gctccacgcc gccaggccgc aaattgttct cacccagtct ccagcaatca tgtctgcatc      120

tccaggggag aaggtcacca tgacctgcag tgccagctca agtgtaagtt acatgaactg      180

gtaccagcag aagtcaggca cctcccccaa aagatggatt tatgacacat ccaaactggc      240

ttctggagtc cctgctcact tcaggggcag tgggtctggg acctcttact ctctcacaat      300

cagcggcatg gaggctgaag atgctgccac ttattactgc cagcagtgga gtagtaaccc      360

attcacgttc ggctcgggga caaagttgga aataaaccgg ggtggtggtg gttctggtgg      420

tggtggttct ggcggcggcg gctccggtgg tggtggatcc gaggtccagc tgcagcagtc      480

tggggctgaa ctggcaagac ctggggcctc agtgaagatg tcctgcaagg cttctggcta      540

cacctttact aggtacacga tgcactgggt aaaacagagg cctggacagg gtctggaatg      600

gattggatac attaatccta gccgtggtta tactaattac aatcagaagt tcaaggacaa      660

ggccacattg actacagaca aatcctccag cacagcctac atgcaactga gcagcctgac      720

atctgaggac tctgcagtct attactgtgc aagatattat gatgatcatt actgccttga      780

ctactggggc caaggcacca ctctcacagt ctcctcagcc aagcccacca cgacgccagc      840

gccgcgacca ccaacaccgg cgcccaccat cgcgtcgcag cccctgtccc tgcgcccaga      900

ggcgtgccgg ccagcggcgg ggggcgcagt gcacacgagg gggctggact tcgcctgtga      960

tatctacatc tgggcgccct tggccgggac ttgtggggtc cttctcctgt cactggttat     1020

caccctttac taactcgag                                                  1039


