                         SEQUENCE LISTING

<110>  Cellectis SA
 
<120>  Universal anti-CD22 CHIMERIC ANTIGEN RECEPTOR ENGINEERED IMMUNE CELLS

<130>  P81701376PCT00

<150>  PA201770239
<151>  2017-03-31

<150>  PA201770240
<151>  2017-03-31

<150>  PA201770542
<151>  2017-06-30

<150>  PCT/EP2017/076800
<151>  2017-10-19

<160>  26

<210>  1

<211>  21
<212>  PRT
<213>  homo sapiens

<220>
<223>  CD8alpha signal peptide

<400>  1
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210>  2

<211>  20
<212>  PRT
<213>  homo sapiens

<220>
<223>  signal peptide

<400>  2
Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly 
            20  


<210>  3

<211>  16
<212>  PRT
<213>  homo sapiens

<220>
<223>  FcgRIIIa hinge

<400>  3
Gly Leu Ala Val Ser Thr Ile Ser Ser Phe Phe Pro Pro Gly Tyr Gln 
1               5                   10                  15      



<210>  4

<211>  45
<212>  PRT
<213>  homo sapiens

<220>
<223>  CD8alpha hinge

<400>  4
Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        35                  40                  45  


<210>  5

<211>  231
<212>  PRT
<213>  homo sapiens

<220>
<223>  IgG1 hinge

<400>  5
Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      


Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
            20                  25                  30          


Asp Thr Leu Met Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val Val 
        35                  40                  45              


Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 
    50                  55                  60                  


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
65                  70                  75                  80  


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
                85                  90                  95      


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 
            100                 105                 110         


Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
        115                 120                 125             


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys 
    130                 135                 140                 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
145                 150                 155                 160 


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
                165                 170                 175     


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
            180                 185                 190         


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
        195                 200                 205             


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
    210                 215                 220                 


Leu Ser Leu Ser Pro Gly Lys 
225                 230     


<210>  6

<211>  24
<212>  PRT
<213>  homo sapiens

<220>
<223>  CD8alpha transmembrane domain

<400>  6
Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr Leu Tyr Cys 
            20                  


<210>  7

<211>  27
<212>  PRT
<213>  homo sapiens

<220>
<223>  41BB transmembrane domain

<400>  7
Ile Ile Ser Phe Phe Leu Ala Leu Thr Ser Thr Ala Leu Leu Phe Leu 
1               5                   10                  15      


Leu Phe Phe Leu Thr Leu Arg Phe Ser Val Val 
            20                  25          


<210>  8

<211>  42
<212>  PRT
<213>  homo sapiens

<220>
<223>  Fragment of 4-1BB (residues 214-255)

<400>  8
Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  9

<211>  112
<212>  PRT
<213>  homo sapiens

<220>
<223>  fragment of T-cell surface glycoprotein CD3 zeta chain

<400>  9
Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         



<210>  10

<211>  15
<212>  PRT
<213>  artificial sequence

<220>
<223>  G4Sx3 linker sequence

<400>  10
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  



<210>  11

<211>  1532
<212>  DNA
<213>  homo sapiens

<220>
<223>  UCART22

<400>  11
tgatcctctt gtcccacaga tatccagaac cctgaccctg ccgtgtacca gctgagagaa     60
tggctctgcc cgtcaccgct ctgctgctgc cactggccct gctgctgcac gcagcaagac    120
cacaggtgca gctgcagcag agcggccctg gcctggtgaa gccaagccag acactgtccc    180
tgacctgcgc catcagcggc gattccgtga gctccaactc cgccgcctgg aattggatca    240
ggcagtcccc ttctcggggc ctggagtggc tgggaaggac atactatcgg tctaagtggt    300
acaacgatta tgccgtgtct gtgaagagca gaatcacaat caaccctgac acctccaaga    360
atcagttctc tctgcagctg aatagcgtga caccagagga caccgccgtg tactattgcg    420
ccagggaggt gaccggcgac ctggaggatg cctttgacat ctggggccag ggcacaatgg    480
tgaccgtgtc tagcggagga ggaggatccg gaggaggagg atctggcggc ggcggcagcg    540
atatccagat gacacagtcc ccatcctctc tgagcgcctc cgtgggcgac agagtgacaa    600
tcacctgtag ggcctcccag accatctggt cttacctgaa ctggtatcag cagaggcccg    660
gcaaggcccc taatctgctg atctacgcag caagctccct gcagagcgga gtgccatcca    720
gattctctgg caggggctcc ggcacagact tcaccctgac catctctagc ctgcaggccg    780
aggacttcgc cacctactat tgccagcagt cttatagcat cccccagaca tttggccagg    840
gcaccaagct ggagatcaag accacaaccc cagcaccaag gccacctaca cctgcaccaa    900
ccatcgcctc tcagcccctg agcctgagac ctgaggcatg taggccagca gcaggaggag    960
cagtccatac aaggggtctg gattttgcat gcgacatcta catctgggca cctctggcag   1020
gaacatgtgg cgtgctcctg ctcagcctgg tcatcaccct gtactgcaag agaggcagga   1080
agaagctgct gtatatcttc aagcagccct tcatgcgccc cgtgcagaca acccaggagg   1140
aggatggctg ctcctgtagg ttcccagaag aggaggaggg aggatgtgag ctgcgcgtga   1200
agttttcccg gtctgccgac gcacctgcat accagcaggg ccagaaccag ctgtataacg   1260
agctgaatct gggccggaga gaggagtacg atgtgctgga caagaggcgc ggcagagatc   1320
cagagatggg cggcaagccc cggagaaaga accctcagga gggcctgtac aatgagctgc   1380
agaaggataa gatggccgag gcctattctg agatcggcat gaagggagag aggcgccggg   1440
gcaagggaca cgacggactg taccagggac tgagcacagc caccaaggat acctatgacg   1500
ccctgcatat gcaggcactg cctccaaggt ga                                 1532




<210>  12

<211>  124
<212>  PRT
<213>  artificial sequence

<220>
<223>  971- heavy chain

<400>  12
Gln Val Gln Leu Gln Gln Ser Gly Pro Gly Leu Val Lys Pro Ser Gln 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Ala Ile Ser Gly Asp Ser Val Ser Ser Asn 
            20                  25                  30          


Ser Ala Ala Trp Asn Trp Ile Arg Gln Ser Pro Ser Arg Gly Leu Glu 
        35                  40                  45              


Trp Leu Gly Arg Thr Tyr Tyr Arg Ser Lys Trp Tyr Asn Asp Tyr Ala 
    50                  55                  60                  


Val Ser Val Lys Ser Arg Ile Thr Ile Asn Pro Asp Thr Ser Lys Asn 
65                  70                  75                  80  


Gln Phe Ser Leu Gln Leu Asn Ser Val Thr Pro Glu Asp Thr Ala Val 
                85                  90                  95      


Tyr Tyr Cys Ala Arg Glu Val Thr Gly Asp Leu Glu Asp Ala Phe Asp 
            100                 105                 110         


Ile Trp Gly Gln Gly Thr Met Val Thr Val Ser Ser 
        115                 120                 


<210>  13

<211>  107
<212>  PRT
<213>  artificial sequence

<220>
<223>  971- light chain

<400>  13
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Thr Ile Trp Ser Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Arg Pro Gly Lys Ala Pro Asn Leu Leu Ile 
        35                  40                  45              


Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Arg Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Tyr Ser Ile Pro Gln 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  14

<211>  464
<212>  PRT
<213>  artificial sequence

<220>
<223>  971-v1 polypeptide CAR sequence

<400>  14
Gln Val Gln Leu Gln Gln Ser Gly Gly Gly Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Val Thr Ser Gly Phe Thr Phe Arg Lys Phe 
            20                  25                  30          


Gly Met Ser Trp Val Arg Gln Thr Ser Asp Lys Arg Leu Glu Trp Val 
        35                  40                  45              


Ala Ser Ile Ser Thr Gly Gly Tyr Asn Thr Tyr Tyr Ser Asp Asn Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Glu Asn Ala Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Lys Ser Glu Asp Thr Ala Leu Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Gly Tyr Ser Ser Thr Ser Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Thr Val Thr Val Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
        115                 120                 125             


Gly Gly Gly Gly Ser Asp Ile Glu Leu Thr Gln Ser Pro Ala Ser Leu 
    130                 135                 140                 


Ser Val Ala Thr Gly Glu Lys Val Thr Ile Arg Cys Met Thr Ser Thr 
145                 150                 155                 160 


Asp Ile Asp Asp Asp Met Asn Trp Tyr Gln Gln Lys Pro Gly Glu Pro 
                165                 170                 175     


Pro Lys Phe Leu Ile Ser Glu Gly Asn Thr Leu Arg Pro Gly Val Pro 
            180                 185                 190         


Ser Arg Phe Ser Ser Ser Gly Thr Gly Thr Asp Phe Val Phe Thr Ile 
        195                 200                 205             


Glu Asn Thr Leu Ser Glu Asp Val Gly Asp Tyr Tyr Cys Leu Gln Ser 
    210                 215                 220                 


Phe Asn Val Pro Leu Thr Phe Gly Asp Gly Thr Lys Leu Glu Lys Ala 
225                 230                 235                 240 


Leu Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile 
                245                 250                 255     


Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala 
            260                 265                 270         


Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr 
        275                 280                 285             


Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu 
    290                 295                 300                 


Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile 
305                 310                 315                 320 


Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp 
                325                 330                 335     


Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
            340                 345                 350         


Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
        355                 360                 365             


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
    370                 375                 380                 


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
385                 390                 395                 400 


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
                405                 410                 415     


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
            420                 425                 430         


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
        435                 440                 445             


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
    450                 455                 460                 





<210>  15

<211>  650
<212>  PRT
<213>  artificial sequence

<220>
<223>  971-v3 polypeptide CAR sequence

<400>  15
Gln Val Gln Leu Gln Gln Ser Gly Gly Gly Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Val Thr Ser Gly Phe Thr Phe Arg Lys Phe 
            20                  25                  30          


Gly Met Ser Trp Val Arg Gln Thr Ser Asp Lys Arg Leu Glu Trp Val 
        35                  40                  45              


Ala Ser Ile Ser Thr Gly Gly Tyr Asn Thr Tyr Tyr Ser Asp Asn Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Glu Asn Ala Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Lys Ser Glu Asp Thr Ala Leu Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Gly Tyr Ser Ser Thr Ser Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Thr Val Thr Val Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
        115                 120                 125             


Gly Gly Gly Gly Ser Asp Ile Glu Leu Thr Gln Ser Pro Ala Ser Leu 
    130                 135                 140                 


Ser Val Ala Thr Gly Glu Lys Val Thr Ile Arg Cys Met Thr Ser Thr 
145                 150                 155                 160 


Asp Ile Asp Asp Asp Met Asn Trp Tyr Gln Gln Lys Pro Gly Glu Pro 
                165                 170                 175     


Pro Lys Phe Leu Ile Ser Glu Gly Asn Thr Leu Arg Pro Gly Val Pro 
            180                 185                 190         


Ser Arg Phe Ser Ser Ser Gly Thr Gly Thr Asp Phe Val Phe Thr Ile 
        195                 200                 205             


Glu Asn Thr Leu Ser Glu Asp Val Gly Asp Tyr Tyr Cys Leu Gln Ser 
    210                 215                 220                 


Phe Asn Val Pro Leu Thr Phe Gly Asp Gly Thr Lys Leu Glu Lys Ala 
225                 230                 235                 240 


Leu Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
                245                 250                 255     


Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            260                 265                 270         


Lys Asp Thr Leu Met Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val 
        275                 280                 285             


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    290                 295                 300                 


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
305                 310                 315                 320 


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                325                 330                 335     


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            340                 345                 350         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        355                 360                 365             


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    370                 375                 380                 


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
385                 390                 395                 400 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                405                 410                 415     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            420                 425                 430         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        435                 440                 445             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    450                 455                 460                 


Ser Leu Ser Leu Ser Pro Gly Lys Ile Tyr Ile Trp Ala Pro Leu Ala 
465                 470                 475                 480 


Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys 
                485                 490                 495     


Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
            500                 505                 510         


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
        515                 520                 525             


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg 
    530                 535                 540                 


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
545                 550                 555                 560 


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
                565                 570                 575     


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
            580                 585                 590         


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
        595                 600                 605             


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
    610                 615                 620                 


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
625                 630                 635                 640 


Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                645                 650 


<210>  16

<211>  530
<212>  PRT
<213>  artificial sequence

<220>
<223>  TAL binding domain  TRAC_T01-L

<400>  16
Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys 
1               5                   10                  15      


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala 
            20                  25                  30          


His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly 
        35                  40                  45              


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
    50                  55                  60                  


Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn 
65                  70                  75                  80  


Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
                85                  90                  95      


Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala 
            100                 105                 110         


Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
        115                 120                 125             


Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala 
    130                 135                 140                 


Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
145                 150                 155                 160 


Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val 
                165                 170                 175     


Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val 
            180                 185                 190         


Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu 
        195                 200                 205             


Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu 
    210                 215                 220                 


Thr Val Gln Ala Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr 
225                 230                 235                 240 


Pro Glu Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala 
                245                 250                 255     


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly 
            260                 265                 270         


Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys 
        275                 280                 285             


Gln Ala Leu Glu Thr Val Gln Ala Leu Leu Pro Val Leu Cys Gln Ala 
    290                 295                 300                 


His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly 
305                 310                 315                 320 


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
                325                 330                 335     


Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn 
            340                 345                 350         


Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Ala Leu Leu Pro Val 
        355                 360                 365             


Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala 
    370                 375                 380                 


Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
385                 390                 395                 400 


Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala 
                405                 410                 415     


Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Ala 
            420                 425                 430         


Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val 
        435                 440                 445             


Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val 
    450                 455                 460                 


Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu 
465                 470                 475                 480 


Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu 
                485                 490                 495     


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr 
            500                 505                 510         


Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Arg Pro Ala 
        515                 520                 525             


Leu Glu 
    530 


<210>  17

<211>  530
<212>  PRT
<213>  artificial sequence

<220>
<223>  TAL binding domain TRAC_T01-R

<400>  17
Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys 
1               5                   10                  15      


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala 
            20                  25                  30          


His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly 
        35                  40                  45              


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
    50                  55                  60                  


Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser His 
65                  70                  75                  80  


Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
                85                  90                  95      


Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala 
            100                 105                 110         


Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Ala Leu Leu 
        115                 120                 125             


Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala 
    130                 135                 140                 


Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
145                 150                 155                 160 


Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val 
                165                 170                 175     


Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val 
            180                 185                 190         


Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln 
        195                 200                 205             


Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu 
    210                 215                 220                 


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr 
225                 230                 235                 240 


Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala 
                245                 250                 255     


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly 
            260                 265                 270         


Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys 
        275                 280                 285             


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala 
    290                 295                 300                 


His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly 
305                 310                 315                 320 


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
                325                 330                 335     


Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn 
            340                 345                 350         


Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Ala Leu Leu Pro Val 
        355                 360                 365             


Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala 
    370                 375                 380                 


Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
385                 390                 395                 400 


Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala 
                405                 410                 415     


Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Ala 
            420                 425                 430         


Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val 
        435                 440                 445             


Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val 
    450                 455                 460                 


Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln 
465                 470                 475                 480 


Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu 
                485                 490                 495     


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr 
            500                 505                 510         


Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Arg Pro Ala 
        515                 520                 525             


Leu Glu 
    530 



<210>  18

<211>  49
<212>  DNA
<213>  artificial sequence

<220>
<223>  target TALEN TRAC_T01

<400>  18
ttgtcccaca gatatccaga accctgaccc tgccgtgtac cagctgaga                 49


<210>  19

<211>  9
<212>  PRT
<213>  artificial sequence

<220>
<223>  Mimotope of CD20 antigen

<400>  19
Cys Pro Tyr Ser Asn Pro Ser Leu Cys 
1               5                   

<210>  20

<211>  1473
<212>  DNA
<213>  artificial sequence

<220>
<223>  CAR 11 dis 16

<400>  20
atggctctgc ccgtcaccgc tctgctgctg ccactggccc tgctgctgca cgcagcaaga     60
ccacaggtgc agctgcagca gagcggccct ggcctggtga agccaagcca gacactgtcc    120
ctgacctgcg ccatcagcgg cgattccgtg agctccaact ccgccgcctg gaattggatc    180
aggcagtccc cttctcgggg cctggagtgg ctgggaagga catactatcg gtctaagtgg    240
tacaacgatt atgccgtgtc tgtgaagagc agaatcacaa tcaaccctga cacctccaag    300
aatcagttct ctctgcagct gaatagcgtg acaccagagg acaccgccgt gtactattgc    360
gccagggagg tgaccggcga cctggaggat gcctttgaca tctggggcca gggcacaatg    420
gtgaccgtgt ctagcggagg aggaggatcc ggaggaggag gatctggcgg cggcggcagc    480
gatatccaga tgacacagtc cccatcctct ctgagcgcct ccgtgggcga cagagtgaca    540
atcacctgta gggcctccca gaccatctgg tcttacctga actggtatca gcagaggccc    600
ggcaaggccc ctaatctgct gatctacgca gcaagctccc tgcagagcgg agtgccatcc    660
agattctctg gcaggggctc cggcacagac ttcaccctga ccatctctag cctgcaggcc    720
gaggacttcg ccacctacta ttgccagcag tcttatagca tcccccagac atttggccag    780
ggcaccaagc tggagatcaa gaccacaacc ccagcaccaa ggccacctac acctgcacca    840
accatcgcct ctcagcccct gagcctgaga cctgaggcat gtaggccagc agcaggagga    900
gcagtccata caaggggtct ggattttgca tgcgacatct acatctgggc acctctggca    960
ggaacatgtg gcgtgctcct gctcagcctg gtcatcaccc tgtactgcaa gagaggcagg   1020
aagaagctgc tgtatatctt caagcagccc ttcatgcgcc ccgtgcagac aacccaggag   1080
gaggatggct gctcctgtag gttcccagaa gaggaggagg gaggatgtga gctgcgcgtg   1140
aagttttccc ggtctgccga cgcacctgca taccagcagg gccagaacca gctgtataac   1200
gagctgaatc tgggccggag agaggagtac gatgtgctgg acaagaggcg cggcagagat   1260
ccagagatgg gcggcaagcc ccggagaaag aaccctcagg agggcctgta caatgagctg   1320
cagaaggata agatggccga ggcctattct gagatcggca tgaagggaga gaggcgccgg   1380
ggcaagggac acgacggact gtaccaggga ctgagcacag ccaccaagga tacctatgac   1440
gccctgcata tgcaggcact gcctccaagg tga                                1473


<210>  21

<211>  59
<212>  DNA
<213>  artificial sequence

<220>
<223>  TARGET TALEN(R)

<400>  21
tgatcctctt gtcccacaga tatccagaac cctgaccctg ccgtgtacca gctgagaga      59


<210>  22

<211>  1473
<212>  DNA
<213>  Artificial sequence

<220>
<223>  971-v3 polynucleotide CAR sequence

<400>  22
atggctctgc ccgtcaccgc tctgctgctg ccactggccc tgctgctgca cgcagcaaga     60
ccacaggtgc agctgcagca gagcggccct ggcctggtga agccaagcca gacactgtcc    120
ctgacctgcg ccatcagcgg cgattccgtg agctccaact ccgccgcctg gaattggatc    180
aggcagtccc cttctcgggg cctggagtgg ctgggaagga catactatcg gtctaagtgg    240
tacaacgatt atgccgtgtc tgtgaagagc agaatcacaa tcaaccctga cacctccaag    300
aatcagttct ctctgcagct gaatagcgtg acaccagagg acaccgccgt gtactattgc    360
gccagggagg tgaccggcga cctggaggat gcctttgaca tctggggcca gggcacaatg    420
gtgaccgtgt ctagcggagg aggaggatcc ggaggaggag gatctggcgg cggcggcagc    480
gatatccaga tgacacagtc cccatcctct ctgagcgcct ccgtgggcga cagagtgaca    540
atcacctgta gggcctccca gaccatctgg tcttacctga actggtatca gcagaggccc    600
ggcaaggccc ctaatctgct gatctacgca gcaagctccc tgcagagcgg agtgccatcc    660
agattctctg gcaggggctc cggcacagac ttcaccctga ccatctctag cctgcaggcc    720
gaggacttcg ccacctacta ttgccagcag tcttatagca tcccccagac atttggccag    780
ggcaccaagc tggagatcaa gaccacaacc ccagcaccaa ggccacctac acctgcacca    840
accatcgcct ctcagcccct gagcctgaga cctgaggcat gtaggccagc agcaggagga    900
gcagtccata caaggggtct ggattttgca tgcgacatct acatctgggc acctctggca    960
ggaacatgtg gcgtgctcct gctcagcctg gtcatcaccc tgtactgcaa gagaggcagg   1020
aagaagctgc tgtatatctt caagcagccc ttcatgcgcc ccgtgcagac aacccaggag   1080
gaggatggct gctcctgtag gttcccagaa gaggaggagg gaggatgtga gctgcgcgtg   1140
aagttttccc ggtctgccga cgcacctgca taccagcagg gccagaacca gctgtataac   1200
gagctgaatc tgggccggag agaggagtac gatgtgctgg acaagaggcg cggcagagat   1260
ccagagatgg gcggcaagcc ccggagaaag aaccctcagg agggcctgta caatgagctg   1320
cagaaggata agatggccga ggcctattct gagatcggca tgaagggaga gaggcgccgg   1380
ggcaagggac acgacggact gtaccaggga ctgagcacag ccaccaagga tacctatgac   1440
gccctgcata tgcaggcact gcctccaagg tga                                1473


<210>  23

<211>  489
<212>  PRT
<213>  artificial sequence

<220>
<223>  CAR distal CD22

<400>  23
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu 
            20                  25                  30          


Val Lys Pro Gly Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe 
        35                  40                  45              


Ala Phe Ser Ile Tyr Asp Met Ser Trp Val Arg Gln Thr Pro Glu Lys 
    50                  55                  60                  


Arg Leu Glu Trp Val Ala Tyr Ile Ser Ser Gly Gly Gly Thr Tyr Tyr 
65                  70                  75                  80  


Pro Asp Thr Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                85                  90                  95      


Asn Thr Leu Tyr Leu Gln Met Ser Ser Leu Lys Ser Glu Asp Thr Ala 
            100                 105                 110         


Met Tyr Tyr Cys Ala Arg His Ser Gly Tyr Gly Thr His Trp Gly Val 
        115                 120                 125             


Leu Phe Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile 
145                 150                 155                 160 


Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly Asp Arg 
                165                 170                 175     


Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Asn Tyr Leu Asn 
            180                 185                 190         


Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile Tyr Tyr 
        195                 200                 205             


Thr Ser Ile Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly 
    210                 215                 220                 


Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln Glu Asp 
225                 230                 235                 240 


Phe Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Trp Thr Phe 
                245                 250                 255     


Gly Gly Gly Thr Lys Leu Glu Ile Lys Ala Thr Thr Thr Pro Ala Pro 
            260                 265                 270         


Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu 
        275                 280                 285             


Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg 
    290                 295                 300                 


Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly 
305                 310                 315                 320 


Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys 
                325                 330                 335     


Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg 
            340                 345                 350         


Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro 
        355                 360                 365             


Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser 
    370                 375                 380                 


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
385                 390                 395                 400 


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
                405                 410                 415     


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
            420                 425                 430         


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
        435                 440                 445             


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
    450                 455                 460                 


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
465                 470                 475                 480 


Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 


<210>  24

<211>  1470
<212>  DNA
<213>  artificial sequence

<220>
<223>  CAR distal CD22

<400>  24
atggctctgc ccgtcaccgc tctgctgctg ccactggccc tgctgctgca cgcagcaagg     60
cctgaggtgc agctggtgga atccggagga ggcctggtga agcctggcgg ctctctgaag    120
ctgagctgtg ccgcctccgg cttcgccttt tccatctacg acatgtcttg ggtgaggcag    180
accccagaga agcgcctgga gtgggtggcc tatatcagct ccggcggcgg cacctactat    240
cccgacacag tgaagggccg gttcaccatc tctagagata acgccaagaa tacactgtac    300
ctgcagatgt ctagcctgaa gagcgaggat accgccatgt actattgcgc aaggcactcc    360
ggatacggaa cacactgggg cgtgctgttt gcctattggg gccagggcac cctggtgaca    420
gtgagcgccg gaggaggagg aagcggcgga ggaggctccg gcggcggcgg ctctgacatc    480
cagatgaccc agaccacatc ctctctgagc gcctccctgg gcgacagggt gacaatctct    540
tgtagagcca gccaggatat ctccaactac ctgaattggt atcagcagaa gcctgatggc    600
accgtgaagc tgctgatcta ctatacatct atcctgcaca gcggagtgcc atcccggttc    660
tctggaagcg gatccggaac cgactactct ctgacaatca gcaacctgga gcaggaggat    720
ttcgccacct atttttgcca gcagggcaat accctgcctt ggacatttgg cggcggcaca    780
aagctggaga tcaaggccac cacaacccct gcaccaaggc caccaacacc agcacctacc    840
atcgcatctc agcctctgag cctgagacca gaggcatgta ggccagcagc aggaggagca    900
gtgcacacaa ggggactgga ttttgcctgt gatatctaca tctgggcacc tctggcagga    960
acatgtggcg tgctcctgct cagcctggtc atcaccctgt actgcaagag aggcaggaag   1020
aagctgctgt atatcttcaa gcagcccttc atgagacccg tgcagacaac ccaggaggag   1080
gacggctgct cctgtaggtt cccagaagag gaggagggag gatgtgagct gcgcgtgaag   1140
ttttcccggt ctgccgatgc acctgcatac cagcagggac agaatcagct gtataacgag   1200
ctgaatctgg gccggagaga ggagtacgac gtgctggata agaggagggg aagggaccca   1260
gagatgggag gcaagcctcg gagaaagaac ccacaggagg gcctgtacaa tgagctgcag   1320
aaggacaaga tggccgaggc ctattctgag atcggcatga agggagagag gcgccggggc   1380
aagggacacg atggcctgta ccagggcctg tccacagcca ccaaggacac ctatgatgcc   1440
ctgcatatgc aggcactgcc tccaaggtga                                    1470


<210>  25

<211>  495
<212>  PRT
<213>  artificial sequence

<220>
<223>  description of artificial sequence: synthetic polypeptide

<220>
<223>  4G7-CAR version 1

<400>  25
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu 
            20                  25                  30          


Ile Lys Pro Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr 
        35                  40                  45              


Thr Phe Thr Ser Tyr Val Met His Trp Val Lys Gln Lys Pro Gly Gln 
    50                  55                  60                  


Gly Leu Glu Trp Ile Gly Tyr Ile Asn Pro Tyr Asn Asp Gly Thr Lys 
65                  70                  75                  80  


Tyr Asn Glu Lys Phe Lys Gly Lys Ala Thr Leu Thr Ser Asp Lys Ser 
                85                  90                  95      


Ser Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Thr Ser Glu Asp Ser 
            100                 105                 110         


Ala Val Tyr Tyr Cys Ala Arg Gly Thr Tyr Tyr Tyr Gly Ser Arg Val 
        115                 120                 125             


Phe Asp Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val 
145                 150                 155                 160 


Met Thr Gln Ala Ala Pro Ser Ile Pro Val Thr Pro Gly Glu Ser Val 
                165                 170                 175     


Ser Ile Ser Cys Arg Ser Ser Lys Ser Leu Leu Asn Ser Asn Gly Asn 
            180                 185                 190         


Thr Tyr Leu Tyr Trp Phe Leu Gln Arg Pro Gly Gln Ser Pro Gln Leu 
        195                 200                 205             


Leu Ile Tyr Arg Met Ser Asn Leu Ala Ser Gly Val Pro Asp Arg Phe 
    210                 215                 220                 


Ser Gly Ser Gly Ser Gly Thr Ala Phe Thr Leu Arg Ile Ser Arg Val 
225                 230                 235                 240 


Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gln His Leu Glu Tyr 
                245                 250                 255     


Pro Phe Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys Arg Ala Asp 
            260                 265                 270         


Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
        275                 280                 285             


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
    290                 295                 300                 


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile 
305                 310                 315                 320 


Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val 
                325                 330                 335     


Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe 
            340                 345                 350         


Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly 
        355                 360                 365             


Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg 
    370                 375                 380                 


Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln 
385                 390                 395                 400 


Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp 
                405                 410                 415     


Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
            420                 425                 430         


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
        435                 440                 445             


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
    450                 455                 460                 


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
465                 470                 475                 480 


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 490                 495 


<210>  26

<211>  495
<212>  PRT
<213>  artificial sequence

<220>
<223>  description of artificial sequence: synthetic polypeptide

<220>
<223>  4G7-CAR version 2

<400>  26
Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Ile 
            20                  25                  30          


Lys Pro Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr 
        35                  40                  45              


Phe Thr Ser Tyr Val Met His Trp Val Lys Gln Lys Pro Gly Gln Gly 
    50                  55                  60                  


Leu Glu Trp Ile Gly Tyr Ile Asn Pro Tyr Asn Asp Gly Thr Lys Tyr 
65                  70                  75                  80  


Asn Glu Lys Phe Lys Gly Lys Ala Thr Leu Thr Ser Asp Lys Ser Ser 
                85                  90                  95      


Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Arg Gly Thr Tyr Tyr Tyr Gly Ser Arg Val Phe 
        115                 120                 125             


Asp Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Gly Gly Gly 
    130                 135                 140                 


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Met 
145                 150                 155                 160 


Thr Gln Ala Ala Pro Ser Ile Pro Val Thr Pro Gly Glu Ser Val Ser 
                165                 170                 175     


Ile Ser Cys Arg Ser Ser Lys Ser Leu Leu Asn Ser Asn Gly Asn Thr 
            180                 185                 190         


Tyr Leu Tyr Trp Phe Leu Gln Arg Pro Gly Gln Ser Pro Gln Leu Leu 
        195                 200                 205             


Ile Tyr Arg Met Ser Asn Leu Ala Ser Gly Val Pro Asp Arg Phe Ser 
    210                 215                 220                 


Gly Ser Gly Ser Gly Thr Ala Phe Thr Leu Arg Ile Ser Arg Val Glu 
225                 230                 235                 240 


Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gln His Leu Glu Tyr Pro 
                245                 250                 255     


Phe Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys Arg Ser Asp Pro 
            260                 265                 270         


Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
        275                 280                 285             


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
    290                 295                 300                 


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile 
305                 310                 315                 320 


Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val 
                325                 330                 335     


Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe 
            340                 345                 350         


Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly 
        355                 360                 365             


Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg 
    370                 375                 380                 


Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln 
385                 390                 395                 400 


Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp 
                405                 410                 415     


Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
            420                 425                 430         


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
        435                 440                 445             


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
    450                 455                 460                 


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
465                 470                 475                 480 


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 490                 495 














