                         SEQUENCE LISTING

<110>  LUPIN LIMITED
 
<120>  EXPRESSION AND LARGE SCALE PRODUCTION OF PEPTIDE

<130>  PCT00743

<150>  201721009672
<151>  2017-03-20

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence for the K34R GLP-1 (7-37) monomer

<400>  1

His Ala Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
            20                  25                  30      


<210>  2
<211>  999
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleic acid sequence for the K34R GLP-1 (7-37) monomer codon 
       optimized for E. coli

<400>  2
atgaaacgtc acgcggaagg cacctttacg tccgatgtga gctcttatct ggaaggccag       60

gcggccaaag aatttattgc ctggctggtc cgtggccgcg gtaaacgtca tgccgaaggc      120

acctttacga gcgacgtgag ttcctacctg gaaggtcaag cagctaaaga atttatcgca      180

tggctggttc gtggccgcgg caaacgccac gctgaaggca cctttacgtc tgatgtctca      240

tcgtatctgg aaggccaagc cgcgaaagaa tttatcgcct ggctggtgcg tggccgcggc      300

aaacgtcacg cagaaggcac cttcacgagt gacgttagct cttacctgga aggccaggcc      360

gccaaagaat ttattgcttg gttagttcgt ggccgcggta aacgccatgc cgaaggcacc      420

ttcacgtccg atgtgagttc ctatctggaa ggccaagctg ccaaagaatt tatcgcttgg      480

ttagtgcgtg gccgcggaaa gcgccacgcg gaaggcacct tcacgtcaga cgtctcatcg      540

tacctggaag gccaggcggc gaaagaattt atcgcgtggt tagtacgtgg ccgcggaaaa      600

cgccacgccg agggcacctt tacgtcggat gttagctctt atctggaagg ccaagcagcg      660

aaagaattta ttgcatggtt ggttcgtggc cgcggaaagc gtcatgcaga gggcaccttt      720

acgagcgatg tgagttccta cctggaaggg caggccgcta aggaatttat cgcgtggctt      780

gttcgtggcc gcggaaaacg tcatgcggag ggcaccttta cgtctgacgt ctcatcgtat      840

ctggaaggcc aggccgcgaa ggaatttatc gcctggttag tccgtggccg cggcaagcgc      900

catgcggagg gcaccttcac gagcgacgtt agctcttacc tggaaggtca agcggcgaaa      960

gaatttattg cgtggctggt ccgtggtcgt ggctaatga                             999


<210>  3
<211>  331
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence for K34R GLP-1 (7-37) concatemer

<400>  3

Met Lys Arg His Ala Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr 
1               5                   10                  15      


Leu Glu Gly Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly 
            20                  25                  30          


Arg Gly Lys Arg His Ala Glu Gly Thr Phe Thr Ser Asp Val Ser Ser 
        35                  40                  45              


Tyr Leu Glu Gly Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg 
    50                  55                  60                  


Gly Arg Gly Lys Arg His Ala Glu Gly Thr Phe Thr Ser Asp Val Ser 
65                  70                  75                  80  


Ser Tyr Leu Glu Gly Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val 
                85                  90                  95      


Arg Gly Arg Gly Lys Arg His Ala Glu Gly Thr Phe Thr Ser Asp Val 
            100                 105                 110         


Ser Ser Tyr Leu Glu Gly Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu 
        115                 120                 125             


Val Arg Gly Arg Gly Lys Arg His Ala Glu Gly Thr Phe Thr Ser Asp 
    130                 135                 140                 


Val Ser Ser Tyr Leu Glu Gly Gln Ala Ala Lys Glu Phe Ile Ala Trp 
145                 150                 155                 160 


Leu Val Arg Gly Arg Gly Lys Arg His Ala Glu Gly Thr Phe Thr Ser 
                165                 170                 175     


Asp Val Ser Ser Tyr Leu Glu Gly Gln Ala Ala Lys Glu Phe Ile Ala 
            180                 185                 190         


Trp Leu Val Arg Gly Arg Gly Lys Arg His Ala Glu Gly Thr Phe Thr 
        195                 200                 205             


Ser Asp Val Ser Ser Tyr Leu Glu Gly Gln Ala Ala Lys Glu Phe Ile 
    210                 215                 220                 


Ala Trp Leu Val Arg Gly Arg Gly Lys Arg His Ala Glu Gly Thr Phe 
225                 230                 235                 240 


Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly Gln Ala Ala Lys Glu Phe 
                245                 250                 255     


Ile Ala Trp Leu Val Arg Gly Arg Gly Lys Arg His Ala Glu Gly Thr 
            260                 265                 270         


Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly Gln Ala Ala Lys Glu 
        275                 280                 285             


Phe Ile Ala Trp Leu Val Arg Gly Arg Gly Lys Arg His Ala Glu Gly 
    290                 295                 300                 


Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly Gln Ala Ala Lys 
305                 310                 315                 320 


Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
                325                 330     


