                         SEQUENCE LISTING

<110>  Rigontec GmbH
 
<120>  METHOD FOR DESIGNING RIG-I LIGANDS

<130>  30A-142 918

<150>  EP17162698.9
<151>  2017-03-24

<150>  EP17166320.6
<151>  2017-04-12

<160>  120   

<170>  PatentIn version 3.5

<210>  1
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tail sequence box1 and box2


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (4)..(11)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (15)..(19)
<223>  n is a, c, g, or u

<400>  1
dnwnnnnnnn nunsnnnnn                                                    19


<210>  2
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  consensus tail sequence


<220>
<221>  misc_feature
<222>  (5)..(11)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (15)..(19)
<223>  n is a, c, g, or u

<400>  2
ugaannnnnn nuucnnnnn                                                    19


<210>  3
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  consensus tail sequence with 5-mer in complementary strand


<220>
<221>  misc_feature
<222>  (5)..(11)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  n is a, c, g, or u

<400>  3
ugaannnnnn nuucngavc                                                    19


<210>  4
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  consensus tail sequence with 5-mer and Box1 in complementary 
       strand


<220>
<221>  misc_feature
<222>  (5)..(11)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  n is a, c, g, or u

<400>  4
ugaannnnnn nuucngavc                                                    19


<210>  5
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  consensus tail sequence with 5-mer, Box1, and A9 in complementary
       strand


<220>
<221>  misc_feature
<222>  (5)..(10)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  n is a, c, g, or u

<400>  5
ugaannnnnn uuucngavc                                                    19


<210>  6
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  consensus tail sequence with 5-mer, Box1, A9, and Box 2 in 
       complementary strand


<220>
<221>  misc_feature
<222>  (5)..(11)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  n is a, c, g, or u

<400>  6
gaaannnnnn nuucngavc                                                    19


<210>  7
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  consensus sequence 5-mer and tail


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (10)..(16)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(24)
<223>  n is a, c, g, or u

<400>  7
gbucnugaan nnnnnnuucn nnnn                                              24


<210>  8
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  consensus 5-mer and tail sequence with consensus 5-mer in 
       complementary strand


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (10)..(16)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  n is a, c, g, or u

<400>  8
gbucnugaan nnnnnnuucn gavc                                              24


<210>  9
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  consensus sequence of 5-mer and tail, and 5-mer and Box1 in 
       complementary strand


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (10)..(16)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  n is a, c, g, or u

<400>  9
gbucnugaan nnnnnnuucn gavc                                              24


<210>  10
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  consensus sequence of 5-mer and tail, and 5-mer, Box1 and A9 in 
       complementary strand


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (10)..(15)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  n is a, c, g, or u

<400>  10
gbucnugaan nnnnnuuucn gavc                                              24


<210>  11
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  consensus sequence of 5-mer and tail with 5-mer, Box1, A9, and 
       Box2 in complementary strand


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (10)..(16)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  n is a, c, g, or u

<400>  11
gbucngaaan nnnnnnuucn gavc                                              24


<210>  12
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I agonist


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (9)..(16)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or u

<400>  12
gbucndnwnn nnnnnnwnsn n                                                 21


<210>  13
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I agonist


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (9)..(16)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or u

<400>  13
gucuadnwnn nnnnnnwnsn n                                                 21


<210>  14
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I agonist


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (9)..(16)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or u

<400>  14
guagudnwnn nnnnnnwnsn n                                                 21


<210>  15
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I agonist


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (9)..(16)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or u

<400>  15
gguaadnwnn nnnnnnwnsn n                                                 21


<210>  16
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I agonist


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (9)..(16)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or u

<400>  16
ggcagdnwnn nnnnnnwnsn n                                                 21


<210>  17
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I agonist


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (9)..(16)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or u

<400>  17
gcuucdnwnn nnnnnnwnsn n                                                 21


<210>  18
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I agonist


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (9)..(16)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or u

<400>  18
gcccadnwnn nnnnnnwnsn n                                                 21


<210>  19
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I agonist


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (9)..(16)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or u

<400>  19
gcgcudnwnn nnnnnnwnsn n                                                 21


<210>  20
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I agonist


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (10)..(16)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or u

<400>  20
gbucnugaan nnnnnnuucn n                                                 21


<210>  21
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I agonist


<220>
<221>  misc_feature
<222>  (10)..(16)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or u

<400>  21
gucuaugaan nnnnnnuucn n                                                 21


<210>  22
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I agonist


<220>
<221>  misc_feature
<222>  (10)..(16)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or u

<400>  22
guaguugaan nnnnnnuucn n                                                 21


<210>  23
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I agonist


<220>
<221>  misc_feature
<222>  (10)..(16)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or u

<400>  23
gguaaugaan nnnnnnuucn n                                                 21


<210>  24
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I agonist


<220>
<221>  misc_feature
<222>  (10)..(16)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or u

<400>  24
ggcagugaan nnnnnnuucn n                                                 21


<210>  25
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I agonist


<220>
<221>  misc_feature
<222>  (10)..(16)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or u

<400>  25
gcuucugaan nnnnnnuucn n                                                 21


<210>  26
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I agonist


<220>
<221>  misc_feature
<222>  (10)..(16)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or u

<400>  26
gcccaugaan nnnnnnuucn n                                                 21


<210>  27
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I agonist


<220>
<221>  misc_feature
<222>  (10)..(16)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or u

<400>  27
gcgcuugaan nnnnnnuucn n                                                 21


<210>  28
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  random tail

<400>  28
uggaugguug gcuaggaua                                                    19


<210>  29
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  GFP-2 tail

<400>  29
ugacccugaa guucaucuu                                                    19


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer HPV16E7 forward

<400>  30
agtgtgactc tacgcttcgg                                                   20


<210>  31
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HPV16E7 reverse

<400>  31
tgtgcccatt aacaggtctt                                                   20


<210>  32
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer hIFNbeta forward

<400>  32
gtcactgtgc ctggaccata                                                   20


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer hIFNbeta reverse

<400>  33
agaggcacag gctaggagat                                                   20


<210>  34
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer hbeta-Actin forward

<400>  34
gagaccgcgt ccgcc                                                        15


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer hbeta-Actin reverse

<400>  35
atcatccatg gtgagctggc                                                   20


<210>  36
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  independent 19-mer tail sequence

<400>  36
ugacccugaa guucaucuu                                                    19


<210>  37
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  independent 19-mer tail sequence

<400>  37
ugacccugaa guucaucu                                                     18


<210>  38
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  independent 19-mer tail sequence

<400>  38
ucaaggugaa cuucaagau                                                    19


<210>  39
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  independent 19-mer tail sequence

<400>  39
ggcuacgucc aggagcgca                                                    19


<210>  40
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  121212

<400>  40
gacgcugacc cugaaguuca ucuu                                              24


<210>  41
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  24R40#5

<400>  41
guucuuggau gguuggcuag gaua                                              24


<210>  42
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  biEGFP1-GUUCU-CDR

<400>  42
guucuugacc cugaaguuca ucu                                               23


<210>  43
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  biEGFP2-GUUCU-CDR

<400>  43
guucuucaag gugaacuuca agau                                              24


<210>  44
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  24R60#6

<400>  44
gcucuuggau gguuggcuag gaua                                              24


<210>  45
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  biEGFP1-GCUCU-CDR

<400>  45
gcucuugacc cugaaguuca ucu                                               23


<210>  46
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  biEGFP2-GCUCU-CDR

<400>  46
gcucuucaag gugaacuuca agau                                              24


<210>  47
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  24R80#1

<400>  47
gguccuggau gguuggcuag gaua                                              24


<210>  48
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  biEGFP1-GGUCC-CDR

<400>  48
gguccugacc cugaaguuca uc                                                22


<210>  49
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  biEGFP2-GGUCC-CDR

<400>  49
gguccucaag gugaacuuca agau                                              24


<210>  50
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  24R40#14

<400>  50
gacaauggau gguuggcuag gaua                                              24


<210>  51
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  biEGFP1-GACAA-CDR

<400>  51
gacaaugacc cugaaguuca ucu                                               23


<210>  52
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  biEGFP2-GACAA-CDR

<400>  52
gacaaucaag gugaacuuca agau                                              24


<210>  53
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  24R60#5

<400>  53
gagguuggau gguuggcuag gaua                                              24


<210>  54
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  biEGFP1-GAGGU-CDR

<400>  54
gagguugacc cugaaguuca ucu                                               23


<210>  55
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  biEGFP2-GAGGU-CDR

<400>  55
gagguucaag gugaacuuca agau                                              24


<210>  56
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  24R60#11

<400>  56
gacgauggau gguuggcuag gaua                                              24


<210>  57
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  biEGFP1-GACGA-CDR

<400>  57
gacgaugacc cugaaguuca ucu                                               23


<210>  58
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  biEGFP2-GACGA-CDR

<400>  58
gacgaucaag gugaacuuca agau                                              24


<210>  59
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  biGFP-GUUCU

<400>  59
guucuggcua cguccaggag cgca                                              24


<210>  60
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  biGFP-GAGGU

<400>  60
gagguggcua cguccaggag cgca                                              24


<210>  61
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  biGFP-GACGA

<400>  61
gacgaggcua cguccaggag cgca                                              24


<210>  62
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  biGFP-GGUCC

<400>  62
gguccggcua cguccaggag cgca                                              24


<210>  63
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  biEGFP1-GACAA-CDR

<400>  63
gacaaggcua cguccaggag cgca                                              24


<210>  64
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siEGFP#4

<400>  64
ucaagauccg ccacaacau                                                    19


<210>  65
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siEGFP#5

<400>  65
ggcuacgucc aggagcgca                                                    19


<210>  66
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5-mer and tail sequence; Box1 and A9 in complementary strand


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (11)..(15)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or u

<400>  66
gbucnugaaa nnnnnuuucn n                                                 21


<210>  67
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tail sequence; 5-mer, Box1 and Box2 in complementary strand


<220>
<221>  misc_feature
<222>  (5)..(10)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  n is a, c, g, or u

<400>  67
gaaannnnnn uucangavc                                                    19


<210>  68
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tail sequence; 5-mer, Box1, A9, and Box2 in complementary strand


<220>
<221>  misc_feature
<222>  (5)..(9)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  n is a, c, g, or u

<400>  68
gaaannnnnu uucangavc                                                    19


<210>  69
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5-mer and tailsequence; 5-mer, Box 1, and Box 2 in complementary 
       strand


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (10)..(16)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  n is a, c, g, or u

<400>  69
gbucngaaan nnnnnnuucn gavc                                              24


<210>  70
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5-mer and tailsequence; 5-mer, Box 1, A9 and Box 2 in 
       complementary strand


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (10)..(15)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  n is a, c, g, or u

<400>  70
gbucngaaan nnnnnuuucn gavc                                              24


<210>  71
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  121212 with C7G7

<400>  71
gacgccgacc cugaaguuca ucuu                                              24


<210>  72
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  24R60#11.7

<400>  72
gacgacgguu uuaguuccaa aagu                                              24


<210>  73
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  24R60#5.7

<400>  73
gaggucgguu uuaguuccaa aagu                                              24


<210>  74
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  24R40#14.7

<400>  74
gacaacgguu uuaguuccaa aagu                                              24


<210>  75
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  24R60#1.7

<400>  75
guggucgguu uuaguuccaa aagu                                              24


<210>  76
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  24R60#6.7

<400>  76
gcucucgguu uuaguuccaa aagu                                              24


<210>  77
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  24R60#11.5

<400>  77
gacgagcugu gauuuuugga uuug                                              24


<210>  78
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  24R60#5.5

<400>  78
gaggugcugu gauuuuugga uuug                                              24


<210>  79
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  24R40#14.5

<400>  79
gacaagcugu gauuuuugga uuug                                              24


<210>  80
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  24R60#1.5

<400>  80
guggugcugu gauuuuugga uuug                                              24


<210>  81
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  24R60#6.5

<400>  81
gcucugcugu gauuuuugga uuug                                              24


<210>  82
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  24R60#11.4

<400>  82
gacgagcaau cagcuaaacg uuau                                              24


<210>  83
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  24R60#5.4

<400>  83
gaggugcaau cagcuaaacg uuau                                              24


<210>  84
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  24R40#14.4

<400>  84
gacaagcaau cagcuaaacg uuau                                              24


<210>  85
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  24R60#1.4

<400>  85
guggugcaau cagcuaaacg uuau                                              24


<210>  86
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  24R60#6.4

<400>  86
gcucugcaau cagcuaaacg uuau                                              24


<210>  87
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  biHPV16E6#003-3'dAdA (sense)


<220>
<221>  misc_feature
<222>  (25)..(26)
<223>  desoxy A

<400>  87
guucuaaaag caaagauucc auaaaa                                            26


<210>  88
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  biHPV16E6#003-3'dAdA (antisense)


<220>
<221>  misc_feature
<222>  (25)..(26)
<223>  desoxy A

<400>  88
uuauggaauc uuugcuuuua gaacaa                                            26


<210>  89
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  biHPV16E6#003 (sense)

<400>  89
guucuaaaag caaagauucc auaa                                              24


<210>  90
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  biHPV16E6#003 (antisense)

<400>  90
uuauggaauc uuugcuuuua gaac                                              24


<210>  91
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  biHPV16E7#001-3dAdA (sense)


<220>
<221>  misc_feature
<222>  (25)..(26)
<223>  desoxy A

<400>  91
gguccugacu cuacgcuucg guugaa                                            26


<210>  92
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  biHPV16E7#001-3dAdA (antisense)


<220>
<221>  misc_feature
<222>  (25)..(26)
<223>  desoxy A

<400>  92
caaccgaagc guagagucag gaccaa                                            26


<210>  93
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  biHPV16E7#001 (sense)

<400>  93
gguccugacu cuacgcuucg guug                                              24


<210>  94
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  biHPV16E7#001 (antisense)

<400>  94
caaccgaagc guagagucag gacc                                              24


<210>  95
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I Agonist


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (9)..(16)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or u

<400>  95
gbucndnwnn nnnnnnunsn n                                                 21


<210>  96
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I Agonist


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (9)..(16)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or u

<400>  96
gucuadnwnn nnnnnnunsn n                                                 21


<210>  97
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I Agonist


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (9)..(16)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or u

<400>  97
guagudnwnn nnnnnnunsn n                                                 21


<210>  98
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I Agonist


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (9)..(16)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or u

<400>  98
gguaadnwnn nnnnnnunsn n                                                 21


<210>  99
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I Agonist


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (9)..(16)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or u

<400>  99
ggcagdnwnn nnnnnnunsn n                                                 21


<210>  100
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I Agonist


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (9)..(16)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or u

<400>  100
gcuucdnwnn nnnnnnunsn n                                                 21


<210>  101
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I Agonist


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (9)..(16)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or u

<400>  101
gcccadnwnn nnnnnnunsn n                                                 21


<210>  102
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I Agonist


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (9)..(16)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or u

<400>  102
gcgcudnwnn nnnnnnunsn n                                                 21


<210>  103
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I Agonist sense

<400>  103
guucugcaau cagcuaaacg uuau                                              24


<210>  104
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I Agonist sense

<400>  104
guucugcaau cagcuauacg uuau                                              24


<210>  105
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I Agonist antisense

<400>  105
auaacguuua gcugauugca gaac                                              24


<210>  106
<211>  26
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I Agonist antisense

<400>  106
aaauaacguu uagcugauug cagaac                                            26


<210>  107
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RGT522 RGT526 RGT552 RGT555 sense


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'-triphosphate

<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  phosphorothioate linkage between positions 1&2 and 2&3

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  2'-O-methyl

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  2'-fluoro

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  2'-fluoro

<220>
<221>  misc_feature
<222>  (22)..(24)
<223>  phosphorothioate linkage between positions 22&23 and 23&24

<400>  107
guucugcaau cagcuaaacg uuau                                              24


<210>  108
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RGT568 RGT569 RGT570 RGT571 sense


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'-triphosphate

<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  phosphorothioate linkage between positions 1&2 and 2&3

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  2'-O-methyl

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  2'-fluoro

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  2'-fluoro

<220>
<221>  misc_feature
<222>  (22)..(24)
<223>  phosphorothioate linkage between positions 22&23 and 23&24

<400>  108
guucugcaau cagcuauacg uuau                                              24


<210>  109
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RGT522 RGT568 antisense


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  phosphorothioate linkage between positions 1&2 and 2&3

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  2'-O-methyl

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  2'-fluoro

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  2'-fluoro

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  3'-monophosphate

<400>  109
auaacguuua gcugauugca gaac                                              24


<210>  110
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RGT526 RGT569 antisense


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  phosphorothioate linkage between positions 1&2 and 2&3

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  2'-O-methyl

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  2'-fluoro

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  2'-fluoro

<400>  110
auaacguuua gcugauugca gaac                                              24


<210>  111
<211>  26
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RGT552 RGT570 antisense


<220>
<221>  misc_feature
<222>  (3)..(5)
<223>  phosphorothioate linkage between positions 3&4 and 4&5

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  2'-O-methyl

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  2'-fluoro

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  2'-fluoro

<220>
<221>  misc_feature
<222>  (26)..(26)
<223>  3'-monophospahte

<400>  111
aaauaacguu uagcugauug cagaac                                            26


<210>  112
<211>  26
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RGT555 RGT571 antisense


<220>
<221>  misc_feature
<222>  (3)..(5)
<223>  phosphorothioate linkage between positions 3&4 and 4&5

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  2'-O-methyl

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  2'-fluoro

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  2'-fluoro

<400>  112
aaauaacguu uagcugauug cagaac                                            26


<210>  113
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3P-GFP2 1S2F sense


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'-triphosphate

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  2'-fluoro

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  2'-O-methyl

<220>
<221>  misc_feature
<222>  (22)..(24)
<223>  phosphorothioate linkage

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  2'-fluoro

<400>  113
gacgcugacc cugaaguuca ucuu                                              24


<210>  114
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3P-GFP2 1S2F antisense


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  phosphorothioate linkage

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  2'-fluoro

<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  2'-O-methyl

<400>  114
aagaugaacu ucagggucag cguc                                              24


<210>  115
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I Agonist antisense

<400>  115
auaacguaua gcugauugca gaac                                              24


<210>  116
<211>  26
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I Agonist antisense

<400>  116
aaauaacgua uagcugauug cagaac                                            26


<210>  117
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I Agonist antisense


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  phosphorothioate linkage between positions 1&2 and 2&3

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  2'-O-methyl

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  2'-fluoro

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  2'-fluoro

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  3'-monophosphate

<400>  117
auaacguaua gcugauugca gaac                                              24


<210>  118
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I Agonist antisense


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  phosphorothioate linkage between positions 1&2 and 2&3

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  2'-O-methyl

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  2'-fluoro

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  2'-fluoro

<400>  118
auaacguaua gcugauugca gaac                                              24


<210>  119
<211>  26
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I Agonist antisense


<220>
<221>  misc_feature
<222>  (3)..(5)
<223>  phosphorothioate linkage between positions 3&4 and 4&5

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  2'-O-methyl

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  2'-fluoro

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  2'-fluoro

<220>
<221>  misc_feature
<222>  (26)..(26)
<223>  3'-monophosphate

<400>  119
aaauaacgua uagcugauug cagaac                                            26


<210>  120
<211>  26
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RIG-I Agonist antisense


<220>
<221>  misc_feature
<222>  (3)..(5)
<223>  phosphorothioate linkage between positions 3&4 and 4&5

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  2'-O-methyl

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  2'-fluoro

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  2'-fluoro

<400>  120
aaauaacgua uagcugauug cagaac                                            26


