                         SEQUENCE LISTING

<110>  IFOM Fondazione Istituto FIRC di Oncologia Molecolar
 
<120>  Method of generating 2 cell-like stem cells

<130>  FJS/FP7347669

<150>  EP 17161888.7
<151>  2017-03-20

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  926
<212>  PRT
<213>  Homo sapiens ETAA1

<400>  1

Met Ser Arg Arg Arg Lys His Asp Asp Ser Pro Ser Pro Lys Lys Thr 
1               5                   10                  15      


Pro His Lys Thr Val Ala Ala Glu Glu Cys Gly Ser Val Val Glu Pro 
            20                  25                  30          


Gly Arg Arg Arg Leu Arg Ser Ala Arg Gly Ser Trp Pro Cys Gly Ala 
        35                  40                  45              


Arg Glu Gly Pro Pro Gly Pro Val Arg Gln Arg Glu Gln Pro Pro Thr 
    50                  55                  60                  


Ala Ala Leu Cys Ser Lys Ser Asn Pro Glu Glu Arg Tyr Glu Thr Pro 
65                  70                  75                  80  


Lys Arg Ala Leu Lys Met Asp Ser Leu Ser Ser Ser Phe Ser Ser Pro 
                85                  90                  95      


Asn Asp Pro Asp Gly Gln Asn Asp Ile Phe Trp Asp Gln Asn Ser Pro 
            100                 105                 110         


Leu Thr Lys Gln Leu Gly Lys Gly Arg Lys Lys Gln Ile Tyr Thr Thr 
        115                 120                 125             


Asp Ser Asp Glu Ile Ser His Ile Val Asn Arg Ile Ala Pro Gln Asp 
    130                 135                 140                 


Glu Lys Pro Thr Thr Asn Ser Met Leu Asp Met Trp Ile Gly Glu Thr 
145                 150                 155                 160 


Ala Ile Pro Cys Thr Pro Ser Val Ala Lys Gly Lys Ser Arg Ala Lys 
                165                 170                 175     


Ile Ser Cys Thr Lys Leu Lys Thr Gln Ser Gln Glu Glu Glu Leu Met 
            180                 185                 190         


Lys Leu Ala Lys Gln Phe Asp Lys Asn Met Glu Glu Leu Asp Val Ile 
        195                 200                 205             


Gln Glu Gln Asn Lys Arg Asn Tyr Asp Phe Thr Gln Met Ile Ser Glu 
    210                 215                 220                 


Thr Glu Ile Leu Ser Asn Tyr Lys Asp Asn Ile Gln Met Trp Ser Leu 
225                 230                 235                 240 


His Asn Ile Val Pro Glu Ile Asp Asn Ala Thr Lys Lys Pro Ile Lys 
                245                 250                 255     


Gly Asn Thr Lys Ile Ser Val Ala Asn Asn Gln Asn Ser Ser Gln Lys 
            260                 265                 270         


Pro Phe Asp Gln Ile Ala Glu Ala Ala Phe Asn Ala Ile Phe Asp Gly 
        275                 280                 285             


Ser Thr Gln Lys Cys Ser Gly Gln Leu Ser Gln Glu Leu Pro Glu Ala 
    290                 295                 300                 


Phe Trp Ser Thr Ser Asn Thr Thr Phe Val Lys Thr Asn Ala Leu Lys 
305                 310                 315                 320 


Glu Glu Lys Ile Ile Thr Asn Glu Thr Leu Val Ile Glu Lys Leu Ser 
                325                 330                 335     


Asn Lys Thr Pro Arg Ser Leu Ser Ser Gln Val Asp Thr Pro Ile Met 
            340                 345                 350         


Thr Lys Ser Cys Val Thr Ser Cys Thr Lys Glu Pro Glu Thr Ser Asn 
        355                 360                 365             


Lys Tyr Ile Asp Ala Phe Thr Thr Ser Asp Phe Glu Asp Asp Trp Glu 
    370                 375                 380                 


Asn Leu Leu Gly Ser Glu Pro Phe Ala Met Gln Asn Ile Asp Met Pro 
385                 390                 395                 400 


Glu Leu Phe Pro Ser Lys Thr Ala His Val Thr Asp Gln Lys Glu Ile 
                405                 410                 415     


Cys Thr Phe Asn Ser Lys Thr Val Lys Asn Thr Ser Arg Ala Asn Thr 
            420                 425                 430         


Ser Pro Asp Ala Arg Leu Gly Asp Ser Lys Val Leu Gln Asp Leu Ser 
        435                 440                 445             


Ser Lys Thr Tyr Asp Arg Glu Leu Ile Asp Ala Glu Tyr Arg Phe Ser 
    450                 455                 460                 


Pro Asn Ser Asn Lys Ser Asn Lys Leu Ser Thr Gly Asn Lys Met Lys 
465                 470                 475                 480 


Phe Glu Asn Ser Ser Asn Lys Ile Val Ile Gln Asp Glu Ile Gln Asn 
                485                 490                 495     


Cys Ile Val Thr Ser Asn Leu Thr Lys Ile Lys Glu Asp Ile Leu Thr 
            500                 505                 510         


Asn Ser Thr Glu Ala Ser Glu Arg Lys Ser Ala Leu Asn Thr Arg Tyr 
        515                 520                 525             


Ser Asn Glu Gln Lys Asn Lys Cys Ile Leu Asn Gln Ser Ile Lys Ala 
    530                 535                 540                 


Pro Val Asn Thr Asp Leu Phe Gly Ser Ala Asn Leu Gly Ser Lys Thr 
545                 550                 555                 560 


Ser Val Ser Asn Pro Asn Gln Thr Ser Ala Ser Lys Val Gly Ser Phe 
                565                 570                 575     


Phe Asp Asp Trp Asn Asp Pro Ser Phe Ala Asn Glu Ile Ile Lys Ala 
            580                 585                 590         


Cys His Gln Leu Asp Asn Thr Trp Glu Ala Asp Asp Val Asp Asp Asp 
        595                 600                 605             


Leu Leu Tyr Gln Ala Cys Asp Asp Ile Glu Arg Leu Thr Gln Gln Gln 
    610                 615                 620                 


Asp Ile Arg Lys Asp Ser Lys Thr Ser Glu Ser Ile Cys Glu Ile Asn 
625                 630                 635                 640 


Asn Asn Ser Glu His Gly Ala Lys Leu Thr Gln Gln Gln Asp Ile Arg 
                645                 650                 655     


Lys Asp Ser Lys Thr Ser Glu Ser Ile Cys Glu Ile Asn Asn Asn Ser 
            660                 665                 670         


Glu His Gly Ala Lys Asn Met Phe Ala Ile Ser Lys Gln Gly Ser Asn 
        675                 680                 685             


Leu Val Gln Ser Lys His Leu Asn Pro Gly Ser Ile Ser Val Gln Thr 
    690                 695                 700                 


Ser Leu Thr Asn Ser Ser Gln Ile Asp Lys Pro Met Lys Met Glu Lys 
705                 710                 715                 720 


Gly Glu Met Tyr Gly Asn Ser Pro Arg Phe Leu Gly Ala Thr Asn Leu 
                725                 730                 735     


Thr Met Tyr Ser Lys Ile Ser Asn Cys Gln Ile Asn Asn Leu His Val 
            740                 745                 750         


Ser Tyr Thr Asn Thr Asp Val Pro Ile Gln Val Asn Ser Ser Lys Leu 
        755                 760                 765             


Val Leu Pro Gly Ser Ser Ser Leu Asn Val Thr Ser Asp His Met Asn 
    770                 775                 780                 


Thr Glu Ile Thr Thr Tyr Lys Lys Lys Leu Ser Thr Asn Gln Pro Cys 
785                 790                 795                 800 


His Lys Thr Val Thr Asp Glu Ala Gln Ser Asn Leu Asn Thr Thr Val 
                805                 810                 815     


Gly Phe Ser Lys Phe Thr Phe Thr Arg Met Lys Asn Ser Gln Ile Leu 
            820                 825                 830         


Ser Gln Phe Asn Gln Asn Cys Ile Thr Gly Ser Met Ser Asp Thr Lys 
        835                 840                 845             


Ile Thr Gln Gly Val Glu Lys Lys Lys Gly Val Asn Pro Leu Leu Glu 
    850                 855                 860                 


Glu Ala Val Gly Gln Gln Ser Leu Val Lys Leu Ser Glu Ser Leu Lys 
865                 870                 875                 880 


Gln Ser Ser Lys Glu Glu Glu Glu Lys Asn Arg Lys Cys Ser Pro Glu 
                885                 890                 895     


Glu Ile Gln Arg Lys Arg Gln Glu Ala Leu Val Arg Arg Met Ala Lys 
            900                 905                 910         


Ala Arg Ala Ser Ser Val Asn Ala Ala Pro Thr Ser Phe Leu 
        915                 920                 925     


<210>  2
<211>  175
<212>  PRT
<213>  Homo sapiens ETAA1-ADD

<400>  2

Val Arg Gln Arg Glu Gln Pro Pro Thr Ala Ala Leu Cys Ser Lys Ser 
1               5                   10                  15      


Asn Pro Glu Glu Arg Tyr Glu Thr Pro Lys Arg Ala Leu Lys Met Glu 
            20                  25                  30          


Thr Asp Ser Leu Ser Ser Ser Phe Ser Ser Pro Asn Asp Pro Asp Gly 
        35                  40                  45              


Gln Asn Asp Ile Phe Trp Asp Gln Asn Ser Pro Leu Thr Lys Gln Leu 
    50                  55                  60                  


Gly Lys Gly Arg Lys Lys Gln Ile Tyr Thr Thr Asp Ser Asp Glu Ile 
65                  70                  75                  80  


Ser His Ile Val Asn Arg Ile Ala Pro Gln Asp Glu Lys Pro Thr Thr 
                85                  90                  95      


Asn Ser Met Glu Thr Leu Asp Met Glu Thr Trp Ile Gly Glu Thr Ala 
            100                 105                 110         


Ile Pro Cys Thr Pro Ser Val Ala Lys Gly Lys Ser Arg Ala Lys Ile 
        115                 120                 125             


Ser Cys Thr Lys Leu Lys Thr Gln Ser Gln Glu Glu Glu Leu Met Glu 
    130                 135                 140                 


Thr Lys Leu Ala Lys Gln Phe Asp Lys Asn Met Glu Thr Glu Glu Leu 
145                 150                 155                 160 


Asp Val Ile Gln Glu Gln Asn Lys Arg Asn Tyr Asp Phe Thr Gln 
                165                 170                 175 


<210>  3
<211>  576
<212>  DNA
<213>  Homo sapiens ETAA1

<400>  3
gtgcggcagc gagagcagcc tccgaccgcc gccctgtgca gtaaaagtaa ccccgaggaa       60

aggtatgaaa caccaaagag agcgctgaaa atggactcac tgtcatcttc cttcagttct      120

cctaatgatc cagatggaca gaatgatatc ttttgggatc agaattctcc attgacaaag      180

cagttaggta aaggaagaaa aaaacagatt tacaccacag atagtgatga gatttcacat      240

attgttaatc gtattgctcc tcaggatgaa aaaccaacaa caaattctat gctggacatg      300

tggattggtg aaactgctat tccttgtact cccagtgtag caaaaggaaa atcaagagca      360

aaaatcagct gcacaaagtt aaaaacacaa agtcaagaag aagaacttat gaaactggct      420

aaacaatttg ataaaaatat ggaagagcta gatgtgattc aagagcaaaa caagaggaat      480

tatgatttta cccaggctcg agctgatcca aaaaagaaga gaaaggtaga tccaaaaaag      540

aagagaaagg tagatccaaa aaagaagaga aaggta                                576


<210>  4
<211>  495
<212>  DNA
<213>  Homo sapiens ETAA1-ADD

<400>  4
gtgcggcagc gagagcagcc tccgaccgcc gccctgtgca gtaaaagtaa ccccgaggaa       60

aggtatgaaa caccaaagag agcgctgaaa atggactcac tgtcatcttc cttcagttct      120

cctaatgatc cagatggaca gaatgatatc ttttgggatc agaattctcc attgacaaag      180

cagttaggta aaggaagaaa aaaacagatt tacaccacag atagtgatga gatttcacat      240

attgttaatc gtattgctcc tcaggatgaa aaaccaacaa caaattctat gctggacatg      300

tggattggtg aaactgctat tccttgtact cccagtgtag caaaaggaaa atcaagagca      360

aaaatcagct gcacaaagtt aaaaacacaa agtcaagaag aagaacttat gaaactggct      420

aaacaatttg ataaaaatat ggaagagcta gatgtgattc aagagcaaaa caagaggaat      480

tatgatttta cccag                                                       495


