                         SEQUENCE LISTING

<110>  F. Hoffmann-La Roche AG
 
<120>  METHOD FOR IN VITRO GLYCOENGINEERING OF AN ERYTHROPOIESIS 
       STIMULATING PROTEIN

<130>  P34172-WO (MME)

<150>  EP 17161883.8
<151>  2017-03-20

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  165
<212>  PRT
<213>  Homo sapiens

<400>  1

Ala Pro Pro Arg Leu Ile Cys Asp Ser Arg Val Leu Glu Arg Tyr Leu 
1               5                   10                  15      


Leu Glu Ala Lys Glu Ala Glu Asn Ile Thr Thr Gly Cys Ala Glu His 
            20                  25                  30          


Cys Ser Leu Asn Glu Asn Ile Thr Val Pro Asp Thr Lys Val Asn Phe 
        35                  40                  45              


Tyr Ala Trp Lys Arg Met Glu Val Gly Gln Gln Ala Val Glu Val Trp 
    50                  55                  60                  


Gln Gly Leu Ala Leu Leu Ser Glu Ala Val Leu Arg Gly Gln Ala Leu 
65                  70                  75                  80  


Leu Val Asn Ser Ser Gln Pro Trp Glu Pro Leu Gln Leu His Val Asp 
                85                  90                  95      


Lys Ala Val Ser Gly Leu Arg Ser Leu Thr Thr Leu Leu Arg Ala Leu 
            100                 105                 110         


Gly Ala Gln Lys Glu Ala Ile Ser Pro Pro Asp Ala Ala Ser Ala Ala 
        115                 120                 125             


Pro Leu Arg Thr Ile Thr Ala Asp Thr Phe Arg Lys Leu Phe Arg Val 
    130                 135                 140                 


Tyr Ser Asn Phe Leu Arg Gly Lys Leu Lys Leu Tyr Thr Gly Glu Ala 
145                 150                 155                 160 


Cys Arg Thr Gly Asp 
                165 


<210>  2
<211>  393
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Val Ser Arg Ser Leu Val Gly Ile Leu Met Met Ala Asn Val Phe 
1               5                   10                  15      


Ile Tyr Phe Ile Met Glu Val Ser Lys Ser Ser Ser Gln Glu Lys Asn 
            20                  25                  30          


Gly Lys Gly Glu Val Ile Ile Pro Lys Glu Lys Phe Trp Lys Ile Ser 
        35                  40                  45              


Thr Pro Pro Glu Ala Tyr Trp Asn Arg Glu Gln Glu Lys Leu Asn Arg 
    50                  55                  60                  


Gln Tyr Asn Pro Ile Leu Ser Met Leu Thr Asn Gln Thr Gly Glu Ala 
65                  70                  75                  80  


Gly Arg Leu Ser Asn Ile Ser His Leu Asn Tyr Cys Glu Pro Asp Leu 
                85                  90                  95      


Arg Val Thr Ser Val Val Thr Gly Phe Asn Asn Leu Pro Asp Arg Phe 
            100                 105                 110         


Lys Asp Phe Leu Leu Tyr Leu Arg Cys Arg Asn Tyr Ser Leu Leu Ile 
        115                 120                 125             


Asp Gln Pro Asp Lys Cys Ala Lys Lys Pro Phe Leu Leu Leu Ala Ile 
    130                 135                 140                 


Lys Ser Leu Thr Pro His Phe Ala Arg Arg Gln Ala Ile Arg Glu Ser 
145                 150                 155                 160 


Trp Gly Gln Glu Ser Asn Ala Gly Asn Gln Thr Val Val Arg Val Phe 
                165                 170                 175     


Leu Leu Gly Gln Thr Pro Pro Glu Asp Asn His Pro Asp Leu Ser Asp 
            180                 185                 190         


Met Leu Lys Phe Glu Ser Glu Lys His Gln Asp Ile Leu Met Trp Asn 
        195                 200                 205             


Tyr Arg Asp Thr Phe Phe Asn Leu Ser Leu Lys Glu Val Leu Phe Leu 
    210                 215                 220                 


Arg Trp Val Ser Thr Ser Cys Pro Asp Thr Glu Phe Val Phe Lys Gly 
225                 230                 235                 240 


Asp Asp Asp Val Phe Val Asn Thr His His Ile Leu Asn Tyr Leu Asn 
                245                 250                 255     


Ser Leu Ser Lys Thr Lys Ala Lys Asp Leu Phe Ile Gly Asp Val Ile 
            260                 265                 270         


His Asn Ala Gly Pro His Arg Asp Lys Lys Leu Lys Tyr Tyr Ile Pro 
        275                 280                 285             


Glu Val Val Tyr Ser Gly Leu Tyr Pro Pro Tyr Ala Gly Gly Gly Gly 
    290                 295                 300                 


Phe Leu Tyr Ser Gly His Leu Ala Leu Arg Leu Tyr His Ile Thr Asp 
305                 310                 315                 320 


Gln Val His Leu Tyr Pro Ile Asp Asp Val Tyr Thr Gly Met Cys Leu 
                325                 330                 335     


Gln Lys Leu Gly Leu Val Pro Glu Lys His Lys Gly Phe Arg Thr Phe 
            340                 345                 350         


Asp Ile Glu Glu Lys Asn Lys Asn Asn Ile Cys Ser Tyr Val Asp Leu 
        355                 360                 365             


Met Leu Val His Ser Arg Lys Pro Gln Glu Met Ile Asp Ile Trp Ser 
    370                 375                 380                 


Gln Leu Gln Ser Ala His Leu Lys Cys 
385                 390             


<210>  3
<211>  383
<212>  PRT
<213>  Arabidopsis thaliana

<400>  3

Met Gly Val His Glu Cys Pro Ala Trp Leu Trp Leu Leu Leu Ser Leu 
1               5                   10                  15      


Leu Ser Leu Pro Leu Gly Leu Pro Val Leu Gly Ala Pro Arg Asp Leu 
            20                  25                  30          


Ser Arg Leu Pro Gln Leu Val Gly Val Ser Thr Pro Leu Gln Gly Gly 
        35                  40                  45              


Ser Asn Ser Ala Ala Ala Ile Gly Gln Ser Ser Gly Glu Leu Arg Thr 
    50                  55                  60                  


Gly Gly Ala Arg Pro Pro Pro Pro Leu Gly Ala Ser Ser Gln Pro Arg 
65                  70                  75                  80  


Pro Gly Gly Asp Ser Ser Pro Val Val Asp Ser Gly Pro Gly Pro Ala 
                85                  90                  95      


Ser Asn Leu Thr Ser Val Pro Val Pro His Thr Thr Ala Leu Ser Leu 
            100                 105                 110         


Pro Ala Cys Pro Glu Glu Ser Pro Leu Leu Val Gly Pro Met Leu Ile 
        115                 120                 125             


Glu Phe Asn Met Pro Val Asp Leu Glu Leu Val Ala Lys Gln Asn Pro 
    130                 135                 140                 


Asn Val Lys Met Gly Gly Arg Tyr Ala Pro Arg Asp Cys Val Ser Pro 
145                 150                 155                 160 


His Lys Val Ala Ile Ile Ile Pro Phe Arg Asn Arg Gln Glu His Leu 
                165                 170                 175     


Lys Tyr Trp Leu Tyr Tyr Leu His Pro Val Leu Gln Arg Gln Gln Leu 
            180                 185                 190         


Asp Tyr Gly Ile Tyr Val Ile Asn Gln Ala Gly Asp Thr Ile Phe Asn 
        195                 200                 205             


Arg Ala Lys Leu Leu Asn Val Gly Phe Gln Glu Ala Leu Lys Asp Tyr 
    210                 215                 220                 


Asp Tyr Thr Cys Phe Val Phe Ser Asp Val Asp Leu Ile Pro Met Asn 
225                 230                 235                 240 


Asp His Asn Ala Tyr Arg Cys Phe Ser Gln Pro Arg His Ile Ser Val 
                245                 250                 255     


Ala Met Asp Lys Phe Gly Phe Ser Leu Pro Tyr Val Gln Tyr Phe Gly 
            260                 265                 270         


Gly Val Ser Ala Leu Ser Lys Gln Gln Phe Leu Thr Ile Asn Gly Phe 
        275                 280                 285             


Pro Asn Asn Tyr Trp Gly Trp Gly Gly Glu Asp Asp Asp Ile Phe Asn 
    290                 295                 300                 


Arg Leu Val Phe Arg Gly Met Ser Ile Ser Arg Pro Asn Ala Val Val 
305                 310                 315                 320 


Gly Arg Cys Arg Met Ile Arg His Ser Arg Asp Lys Lys Asn Glu Pro 
                325                 330                 335     


Asn Pro Gln Arg Phe Asp Arg Ile Ala His Thr Lys Glu Thr Met Leu 
            340                 345                 350         


Ser Asp Gly Leu Asn Ser Leu Thr Tyr Gln Val Leu Asp Val Gln Arg 
        355                 360                 365             


Tyr Pro Leu Tyr Thr Gln Ile Thr Val Asp Ile Gly Thr Pro Ser 
    370                 375                 380             


<210>  4
<211>  328
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Gly Val His Glu Cys Pro Ala Trp Leu Trp Leu Leu Leu Ser Leu 
1               5                   10                  15      


Leu Ser Leu Pro Leu Gly Leu Pro Val Leu Gly Val Ala Pro Val Glu 
            20                  25                  30          


Met Lys Arg Arg Asn Lys Ile Gln Pro Cys Leu Ser Lys Pro Ala Phe 
        35                  40                  45              


Ala Ser Leu Leu Arg Phe His Gln Phe His Pro Phe Leu Cys Ala Ala 
    50                  55                  60                  


Asp Phe Arg Lys Ile Ala Ser Leu Tyr Gly Ser Asp Lys Phe Asp Leu 
65                  70                  75                  80  


Pro Tyr Gly Met Arg Thr Ser Ala Glu Tyr Phe Arg Leu Ala Leu Ser 
                85                  90                  95      


Lys Leu Gln Ser Cys Asp Leu Phe Asp Glu Phe Asp Asn Ile Pro Cys 
            100                 105                 110         


Lys Lys Cys Val Val Val Gly Asn Gly Gly Val Leu Lys Asn Lys Thr 
        115                 120                 125             


Leu Gly Glu Lys Ile Asp Ser Tyr Asp Val Ile Ile Arg Met Asn Asn 
    130                 135                 140                 


Gly Pro Val Leu Gly His Glu Glu Glu Val Gly Arg Arg Thr Thr Phe 
145                 150                 155                 160 


Arg Leu Phe Tyr Pro Glu Ser Val Phe Ser Asp Pro Ile His Asn Asp 
                165                 170                 175     


Pro Asn Thr Thr Val Ile Leu Thr Ala Phe Lys Pro His Asp Leu Arg 
            180                 185                 190         


Trp Leu Leu Glu Leu Leu Met Gly Asp Lys Ile Asn Thr Asn Gly Phe 
        195                 200                 205             


Trp Lys Lys Pro Ala Leu Asn Leu Ile Tyr Lys Pro Tyr Gln Ile Arg 
    210                 215                 220                 


Ile Leu Asp Pro Phe Ile Ile Arg Thr Ala Ala Tyr Glu Leu Leu His 
225                 230                 235                 240 


Phe Pro Lys Val Phe Pro Lys Asn Gln Lys Pro Lys His Pro Thr Thr 
                245                 250                 255     


Gly Ile Ile Ala Ile Thr Leu Ala Phe Tyr Ile Cys His Glu Val His 
            260                 265                 270         


Leu Ala Gly Phe Lys Tyr Asn Phe Ser Asp Leu Lys Ser Pro Leu His 
        275                 280                 285             


Tyr Tyr Gly Asn Ala Thr Met Ser Leu Met Asn Lys Asn Ala Tyr His 
    290                 295                 300                 


Asn Val Thr Ala Glu Gln Leu Phe Leu Lys Asp Ile Ile Glu Lys Asn 
305                 310                 315                 320 


Leu Val Ile Asn Leu Thr Gln Asp 
                325             


<210>  5
<211>  346
<212>  PRT
<213>  Homo sapiens

<400>  5

Met Gly Val His Glu Cys Pro Ala Trp Leu Trp Leu Leu Leu Ser Leu 
1               5                   10                  15      


Leu Ser Leu Pro Leu Gly Leu Pro Val Leu Gly Ala Pro Glu Ala Ser 
            20                  25                  30          


Phe Gln Val Trp Asn Lys Asp Ser Ser Ser Lys Asn Leu Ile Pro Arg 
        35                  40                  45              


Leu Gln Lys Ile Trp Lys Asn Tyr Leu Ser Met Asn Lys Tyr Lys Val 
    50                  55                  60                  


Ser Tyr Lys Gly Pro Gly Pro Gly Ile Lys Phe Ser Ala Glu Ala Leu 
65                  70                  75                  80  


Arg Cys His Leu Arg Asp His Val Asn Val Ser Met Val Glu Val Thr 
                85                  90                  95      


Asp Phe Pro Phe Asn Thr Ser Glu Trp Glu Gly Tyr Leu Pro Lys Glu 
            100                 105                 110         


Ser Ile Arg Thr Lys Ala Gly Pro Trp Gly Arg Cys Ala Val Val Ser 
        115                 120                 125             


Ser Ala Gly Ser Leu Lys Ser Ser Gln Leu Gly Arg Glu Ile Asp Asp 
    130                 135                 140                 


His Asp Ala Val Leu Arg Phe Asn Gly Ala Pro Thr Ala Asn Phe Gln 
145                 150                 155                 160 


Gln Asp Val Gly Thr Lys Thr Thr Ile Arg Leu Met Asn Ser Gln Leu 
                165                 170                 175     


Val Thr Thr Glu Lys Arg Phe Leu Lys Asp Ser Leu Tyr Asn Glu Gly 
            180                 185                 190         


Ile Leu Ile Val Trp Asp Pro Ser Val Tyr His Ser Asp Ile Pro Lys 
        195                 200                 205             


Trp Tyr Gln Asn Pro Asp Tyr Asn Phe Phe Asn Asn Tyr Lys Thr Tyr 
    210                 215                 220                 


Arg Lys Leu His Pro Asn Gln Pro Phe Tyr Ile Leu Lys Pro Gln Met 
225                 230                 235                 240 


Pro Trp Glu Leu Trp Asp Ile Leu Gln Glu Ile Ser Pro Glu Glu Ile 
                245                 250                 255     


Gln Pro Asn Pro Pro Ser Ser Gly Met Leu Gly Ile Ile Ile Met Met 
            260                 265                 270         


Thr Leu Cys Asp Gln Val Asp Ile Tyr Glu Phe Leu Pro Ser Lys Arg 
        275                 280                 285             


Lys Thr Asp Val Cys Tyr Tyr Tyr Gln Lys Phe Phe Asp Ser Ala Cys 
    290                 295                 300                 


Thr Met Gly Ala Tyr His Pro Leu Leu Tyr Glu Lys Asn Leu Val Lys 
305                 310                 315                 320 


His Leu Asn Gln Gly Thr Asp Glu Asp Ile Tyr Leu Leu Gly Lys Ala 
                325                 330                 335     


Thr Leu Pro Gly Phe Arg Thr Ile His Cys 
            340                 345     


<210>  6
<211>  382
<212>  PRT
<213>  Clostridium perfringens

<400>  6

Met Cys Asn Lys Asn Asn Thr Phe Glu Lys Asn Leu Asp Ile Ser His 
1               5                   10                  15      


Lys Pro Glu Pro Leu Ile Leu Phe Asn Lys Asp Asn Asn Ile Trp Asn 
            20                  25                  30          


Ser Lys Tyr Phe Arg Ile Pro Asn Ile Gln Leu Leu Asn Asp Gly Thr 
        35                  40                  45              


Ile Leu Thr Phe Ser Asp Ile Arg Tyr Asn Gly Pro Asp Asp His Ala 
    50                  55                  60                  


Tyr Ile Asp Ile Ala Ser Ala Arg Ser Thr Asp Phe Gly Lys Thr Trp 
65                  70                  75                  80  


Ser Tyr Asn Ile Ala Met Lys Asn Asn Arg Ile Asp Ser Thr Tyr Ser 
                85                  90                  95      


Arg Val Met Asp Ser Thr Thr Val Ile Thr Asn Thr Gly Arg Ile Ile 
            100                 105                 110         


Leu Ile Ala Gly Ser Trp Asn Thr Asn Gly Asn Trp Ala Met Thr Thr 
        115                 120                 125             


Ser Thr Arg Arg Ser Asp Trp Ser Val Gln Met Ile Tyr Ser Asp Asp 
    130                 135                 140                 


Asn Gly Leu Thr Trp Ser Asn Lys Ile Asp Leu Thr Lys Asp Ser Ser 
145                 150                 155                 160 


Lys Val Lys Asn Gln Pro Ser Asn Thr Ile Gly Trp Leu Gly Gly Val 
                165                 170                 175     


Gly Ser Gly Ile Val Met Asp Asp Gly Thr Ile Val Met Pro Ala Gln 
            180                 185                 190         


Ile Ser Leu Arg Glu Asn Asn Glu Asn Asn Tyr Tyr Ser Leu Ile Ile 
        195                 200                 205             


Tyr Ser Lys Asp Asn Gly Glu Thr Trp Thr Met Gly Asn Lys Val Pro 
    210                 215                 220                 


Asn Ser Asn Thr Ser Glu Asn Met Val Ile Glu Leu Asp Gly Ala Leu 
225                 230                 235                 240 


Ile Met Ser Thr Arg Tyr Asp Tyr Ser Gly Tyr Arg Ala Ala Tyr Ile 
                245                 250                 255     


Ser His Asp Leu Gly Thr Thr Trp Glu Ile Tyr Glu Pro Leu Asn Gly 
            260                 265                 270         


Lys Ile Leu Thr Gly Lys Gly Ser Gly Cys Gln Gly Ser Phe Ile Lys 
        275                 280                 285             


Ala Thr Thr Ser Asn Gly His Arg Ile Gly Leu Ile Ser Ala Pro Lys 
    290                 295                 300                 


Asn Thr Lys Gly Glu Tyr Ile Arg Asp Asn Ile Ala Val Tyr Met Ile 
305                 310                 315                 320 


Asp Phe Asp Asp Leu Ser Lys Gly Val Gln Glu Ile Cys Ile Pro Tyr 
                325                 330                 335     


Pro Glu Asp Gly Asn Lys Leu Gly Gly Gly Tyr Ser Cys Leu Ser Phe 
            340                 345                 350         


Lys Asn Asn His Leu Gly Ile Val Tyr Glu Ala Asn Gly Asn Ile Glu 
        355                 360                 365             


Tyr Gln Asp Leu Thr Pro Tyr Tyr Ser Leu Ile Asn Lys Gln 
    370                 375                 380         


