﻿SEQUENCE LISTING
<110>  江苏融泰生物技术有限公司
<120>  一种融合蛋白及其制备方法和其在制备治疗眼科疾病、抗炎、抗肿瘤药物中的应用
<160>  7
<170>  Patent In version 3.5
<210>  1
<211>  404
<212>  PRT
<213>  人工序列
<400>  1
Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
1               5                   10                  15      
Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
            20                  25                  30          
Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        35                  40                  45              
Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    50                  55                  60                  
His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
65                  70                  75                  80  
Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                85                  90                  95      
Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            100                 105                 110         
Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        115                 120                 125             
Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 
    130                 135                 140                 
Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
145                 150                 155                 160 
Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                165                 170                 175     
Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
            180                 185                 190         
Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        195                 200                 205             
His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    210                 215                 220                 
Pro Gly Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
225                 230                 235                 240 
Gly Ser His Lys Cys Asp Ile Thr Leu Gln Glu Ile Ile Lys Thr Leu 
                245                 250                 255     
Asn Ser Leu Thr Glu Gln Lys Thr Leu Cys Thr Glu Leu Thr Val Thr 
            260                 265                 270         
Asp Ile Phe Ala Ala Ser Lys Asn Thr Thr Glu Lys Glu Thr Phe Cys 
        275                 280                 285             
Arg Ala Ala Thr Val Leu Arg Gln Phe Tyr Ser His His Glu Lys Asp 
    290                 295                 300                 
Thr Arg Cys Leu Gly Ala Thr Ala Gln Gln Phe His Arg His Lys Gln 
305                 310                 315                 320 
Leu Ile Arg Phe Leu Lys Arg Leu Asp Arg Asn Leu Trp Gly Leu Ala 
                325                 330                 335     
Gly Leu Asn Ser Cys Pro Val Lys Glu Ala Asn Gln Ser Thr Leu Glu 
            340                 345                 350         
Asn Phe Leu Glu Arg Leu Lys Thr Ile Met Asp Glu Lys Asp Ser Lys 
        355                 360                 365             
Cys Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    370                 375                 380                 
Gly Ser Ile Val Arg Arg Ala Asp Arg Ala Ala Val Pro Gly Gly Gly 
385                 390                 395                 400 
Gly Arg Gly Asp 

<210>  2
<211>418
<212>  PRT
<213>人工序列
<400>  2
Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
1               5                   10                  15      
Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
            20                  25                  30          
Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        35                  40                  45              
Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    50                  55                  60                  
His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
65                  70                  75                  80  
Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                85                  90                  95      
Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            100                 105                 110         
Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        115                 120                 125             
Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 
    130                 135                 140                 
Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
145                 150                 155                 160 
Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                165                 170                 175     
Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
            180                 185                 190         
Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        195                 200                 205             
His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    210                 215                 220                 
Pro Gly Lys Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala 
225                 230                 235                 240 
Ala Ala Lys Glu Ala Ala Ala Lys Ala His Lys Cys Asp Ile Thr Leu 
                245                 250                 255     
Gln Glu Ile Ile Lys Thr Leu Asn Ser Leu Thr Glu Gln Lys Thr Leu 
            260                 265                 270         
Cys Thr Glu Leu Thr Val Thr Asp Ile Phe Ala Ala Ser Lys Asn Thr 
        275                 280                 285             
Thr Glu Lys Glu Thr Phe Cys Arg Ala Ala Thr Val Leu Arg Gln Phe 
    290                 295                 300                 
Tyr Ser His His Glu Lys Asp Thr Arg Cys Leu Gly Ala Thr Ala Gln 
305                 310                 315                 320 
Gln Phe His Arg His Lys Gln Leu Ile Arg Phe Leu Lys Arg Leu Asp 
                325                 330                 335     
Arg Asn Leu Trp Gly Leu Ala Gly Leu Asn Ser Cys Pro Val Lys Glu 
            340                 345                 350         
Ala Asn Gln Ser Thr Leu Glu Asn Phe Leu Glu Arg Leu Lys Thr Ile 
        355                 360                 365             
Met Asp Glu Lys Asp Ser Lys Cys Ser Ser Ala Glu Ala Ala Ala Lys 
    370                 375                 380                 
Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala 
385                 390                 395                 400 
Ile Val Arg Arg Ala Asp Arg Ala Ala Val Pro Gly Gly Gly Gly Arg 
                405                 410                 415     
Gly Asp 

<210>  3
<211>1212
<212>  DNA
<213>人工序列
<400>  3
gacaagaccc acacatgccc tccttgtcct gctcctgagc tgctgggcgg accttccgtg       60
ttcctgtttc cccccaagcc caaggatacc ctcatgatca gcaggacccc cgaggtgaca      120
tgcgtggtgg tggatgtcag ccacgaggac cccgaggtga agttcaactg gtatgtcgac      180
ggcgtggagg tgcataacgc caagacaaag cccagggagg agcagtacaa cagcacatat      240
cgggtggtct ccgtgctcac cgtgctgcac caggattggc tcaacggcaa ggagtacaag      300
tgtaaggtgt ccaacaaggc cctccccgcc cccatcgaga agacaatcag caaggctaag      360
ggccaacctc gggagcccca agtctacacc ctgcccccta gcagggacga gctcaccaag      420
aaccaggtca gcctgacatg tctggtgaag ggcttctacc ccagcgacat cgccgtggag      480
tgggaaagca acggacagcc cgagaacaac tacaagacca ccccccccgt gctggacagc      540
gacggcagct tcttcctgta cagcaagctg accgtcgata agagcaggtg gcagcagggc      600
aatgtgttta gctgctccgt gatgcacgag gccctgcata accactacac ccagaagagc      660
ctgagcctgt cccctggaaa gggaggcggc ggatccggcg gcggcggatc cggaggcggc      720
ggctcccata agtgcgacat caccctccag gaaatcatca agaccctgaa cagcctgacc      780
gagcagaaga ccctgtgcac cgagctgacc gtgaccgata tcttcgccgc ttccaagaac      840
accaccgaga aggagacctt ctgtagggcc gccaccgtcc tgaggcagtt ctatagccac      900
cacgagaaag acacccggtg tctgggcgcc acagctcagc agttccaccg gcacaagcag      960
ctgatccggt tcctcaagcg gctggatagg aacctgtggg gcctcgctgg cctgaacagc     1020
tgccccgtga aggaggctaa ccagagcacc ctggagaact tcctggaacg gctgaagacc     1080
atcatggacg aaaaagactc caagtgctcc agcggcggag gcggaagcgg cggcggaggc     1140
tccggaggcg gaggaagcat cgtgaggcgg gctgataggg ctgctgtgcc tggaggagga     1200
ggcaggggcg ac                                                  1212

<210>  4
<211>1254
<212>  DNA
<213>人工序列
<400>  4
gacaaaaccc acacatgccc tccttgtccc gcccctgaac tgctcggcgg ccccagcgtc       60
ttcctgtttc ctcccaagcc taaagacacc ctgatgatca gccggacccc tgaggtcacc      120
tgcgtggtgg tcgatgtgag ccacgaggac cccgaggtga agttcaactg gtacgtcgac      180
ggagtggagg tccataatgc caagacaaag ccccgggagg agcagtacaa ctccacctac      240
agggtggtca gcgtgctgac cgtcctccac caagactggc tcaatggcaa ggaatataaa      300
tgcaaggtga gcaacaaggc cctgcctgcc cccatcgaga agaccatctc caaggccaag      360
ggacagccca gggagcctca ggtgtacacc ctgcctccct ccagggacga gctgacaaag      420
aaccaggtga gcctcacatg cctggtgaag ggcttttatc ccagcgacat cgccgtcgag      480
tgggagagca acggccagcc tgagaacaac tacaagacca cccctcccgt cctcgactcc      540
gatggatcct tcttcctgta cagcaagctg accgtggata agagcaggtg gcagcagggc      600
aacgtgttct cctgtagcgt gatgcatgag gccctgcaca accattacac ccagaaatcc      660
ctgtccctgt cccctggcaa agccgaggcc gccgctaagg aagccgctgc caaagaagcc      720
gctgccaagg aggccgctgc caaagcccac aagtgtgata tcacactgca ggagatcatc      780
aagaccctga atagcctcac cgagcagaag accctgtgca ccgaactgac cgtgaccgac      840
atctttgccg cctccaagaa taccaccgag aaggagacct tctgtcgggc tgccaccgtg      900
ctccggcagt tctacagcca ccacgagaag gacacccggt gcctgggagc taccgctcag      960
cagtttcacc ggcacaagca gctcatcagg ttcctgaaga ggctggatag gaacctgtgg     1020
ggcctggctg gcctgaattc ctgccccgtg aaggaggcta accagtccac cctggagaat     1080
tttctggaga ggctgaagac catcatggat gagaaggaca gcaagtgttc ctccgctgaa     1140
gccgccgcta aggaggccgc cgctaaagag gctgccgcca aggaagctgc cgccaaagct     1200
atcgtgaggc gggccgacag ggctgctgtg cctggcggcg gcggcagggg cgac           1254

<210>  5
<211>  18
<212>  PRT
<213> 人工序列（HM-3）
<400>  5
Ile Val Arg Arg Ala Asp Arg Ala Ala Val Pro Gly Gly Gly Gly Arg 
1            5                10               15
Gly Asp
    18

<210>  6
<211>  129
<212>  PRT
<213> 人工序列（白介素4）
<400>  6
His Lys Cys Asp Ile Thr Leu Gln Glu Ile Ile Lys Thr Leu Asn Ser 
1             5               10              15  
Leu Thr Glu Gln Lys Thr Leu Cys Thr Glu Leu Thr Val Thr Asp Ile 
           20                25              30     
Phe Ala Ala Ser Lys Asn Thr Thr Glu Lys Glu Thr Phe Cys Arg Ala 
       35               35               40            
Ala Thr Val Leu Arg Gln Phe Tyr Ser His His Glu Lys Asp Thr Arg 
    45               50              55                 
Cys Leu Gly Ala Thr Ala Gln Gln Phe His Arg His Lys Gln Leu Ile 
60               65               70                80
Arg Phe Leu Lys Arg Leu Asp Arg Asn Leu Trp Gly Leu Ala Gly Leu
              85                90                95    
Asn Ser Cys Pro Val Lys Glu Ala Asn Gln Ser Thr Leu Glu Asn Phe 
          100              105              110          
Leu Glu Arg Leu Lys Thr Ile Met Asp Glu Lys Asp Ser Lys Cys Ser 
       115             120               125           
Ser

<210>  7
<211>  232
<212>  PRT
<213> 人工序列
<400>  7
Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1             5                10               15      
Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
           20               25               30          
Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        35              40               45              
Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50               55               60                  
Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65               70                75               80  
Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
              85               90               95      
Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
          100               105              110         
Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
       115             120             125             
Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    130              135             140                 
Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
145              150               155              160 
Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
             165               170              175     
Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
          180               185             190         
Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
      195               200              205             
Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
   210              215              220                 
Ser Leu Ser Leu Ser Pro Gly Lys
225              230   


