                               SEQUENCE LISTING

<110> TWIST BIOSCIENCE CORPORATION
 
<120> VARIANT LIBRARIES OF THE IMMUNOLOGICAL SYNAPSE AND SYNTHESIS 
      THEREOF

<130> 44854-733.601

<140>
<141>

<150> 62/471,810
<151> 2017-03-15

<160> 49    

<170> PatentIn version 3.5

<210> 1
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 1
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 2
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 2
atgtttagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 3
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 3
atgttaagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 4
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 4
atgattagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 5
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 5
atgtctagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 6
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 6
atgcctagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 7
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 7
atgactagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 8
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 8
atggctagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 9
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 9
atgtatagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 10
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 10
atgcatagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 11
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 11
atgcaaagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 12
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 12
atgaatagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 13
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 13
atgaaaagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 14
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 14
atggatagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 15
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 15
atggaaagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 16
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 16
atgtgtagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 17
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 17
atgtggagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 18
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 18
atgcgtagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 19
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 19
atgggtagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 20
<211> 62
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 20
agacaatcaa ccatttgggg tggacagcct tgacctctag acttcggcat tttttttttt       60

tt                                                                      62


<210> 21
<211> 112
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 21
cgggatcctt atcgtcatcg tcgtacagat cccgacccat ttgctgtcca ccagtcatgc       60

tagccatacc atgatgatga tgatgatgag aaccccgcat tttttttttt tt              112


<210> 22
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 22
atgcggggtt ctcatcatc                                                    19


<210> 23
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 23
cgggatcctt atcgtcatcg                                                   20


<210> 24
<211> 600
<212> DNA
<213> Homo sapiens

<400> 24
atgaagtcag gcctctggta tttctttctc ttctgcttgc gcattaaagt tttaacagga       60

gaaatcaatg gttctgccaa ttatgagatg tttatatttc acaacggagg tgtacaaatt      120

ttatgcaaat atcctgacat tgtccagcaa tttaaaatgc agttgctgaa aggggggcaa      180

atactctgcg atctcactaa gacaaaagga agtggaaaca cagtgtccat taagagtctg      240

aaattctgcc attctcagtt atccaacaac agtgtctctt tttttctata caacttggac      300

cattctcatg ccaactatta cttctgcaac ctatcaattt ttgatcctcc tccttttaaa      360

gtaactctta caggaggata tttgcatatt tatgaatcac aactttgttg ccagctgaag      420

ttctggttac ccataggatg tgcagccttt gttgtagtct gcattttggg atgcatactt      480

atttgttggc ttacaaaaaa gaagtattca tccagtgtgc acgaccctaa cggtgaatac      540

atgttcatga gagcagtgaa cacagccaaa aaatctagac tcacagatgt gaccctataa      600


<210> 25
<211> 402
<212> DNA
<213> Homo sapiens

<400> 25
atggtatcac atcggtatcc tcgaattcaa agtatcaaag tacaatttac cgaatataag       60

aaggagaaag gtttcatcct cacttcccaa aaggaggatg aaatcatgaa ggtgcagaac      120

aactcagtca tcatcaactg tgatgggttt tatctcatct ccctgaaggg ctacttctcc      180

caggaagtca acattagcct tcattaccag aaggatgagg agcccctctt ccaactgaag      240

aaggtcaggt ctgtcaactc cttgatggtg gcctctctga cttacaaaga caaagtctac      300

ttgaatgtga ccactgacaa tacctccctg gatgacttcc atgtgaatgg cggagaactg      360

attcttatcc atcaaaatcc tggtgaattc tgtgtccttt ga                         402


<210> 26
<211> 768
<212> DNA
<213> Homo sapiens

<400> 26
atgggaaaca gctgttacaa catagtagcc actctgttgc tggtcctcaa ctttgagagg       60

acaagatcat tgcaggatcc ttgtagtaac tgcccagctg gtacattctg tgataataac      120

aggaatcaga tttgcagtcc ctgtcctcca aatagtttct ccagcgcagg tggacaaagg      180

acctgtgaca tatgcaggca gtgtaaaggt gttttcagga ccaggaagga gtgttcctcc      240

accagcaatg cagagtgtga ctgcactcca gggtttcact gcctgggggc aggatgcagc      300

atgtgtgaac aggattgtaa acaaggtcaa gaactgacaa aaaaaggttg taaagactgt      360

tgctttggga catttaacga tcagaaacgt ggcatctgtc gaccctggac aaactgttct      420

ttggatggaa agtctgtgct tgtgaatggg acgaaggaga gggacgtggt ctgtggacca      480

tctccagccg acctctctcc gggagcatcc tctgtgaccc cgcctgcccc tgcgagagag      540

ccaggacact ctccgcagat catctccttc tttcttgcgc tgacgtcgac tgcgttgctc      600

ttcctgctgt tcttcctcac gctccgtttc tctgttgtta aacggggcag aaagaaactc      660

ctgtatatat tcaaacaacc atttatgaga ccagtacaaa ctactcaaga ggaagatggc      720

tgtagctgcc gatttccaga agaagaagaa ggaggatgtg aactgtga                   768


<210> 27
<211> 783
<212> DNA
<213> Homo sapiens

<400> 27
atggcacggc cacatccctg gtggctgtgc gttctgggga ccctggtggg gctctcagct       60

actccagccc ccaagagctg cccagagagg cactactggg ctcagggaaa gctgtgctgc      120

cagatgtgtg agccaggaac attcctcgtg aaggactgtg accagcatag aaaggctgct      180

cagtgtgatc cttgcatacc gggggtctcc ttctctcctg accaccacac ccggccccac      240

tgtgagagct gtcggcactg taactctggt cttctcgttc gcaactgcac catcactgcc      300

aatgctgagt gtgcctgtcg caatggctgg cagtgcaggg acaaggagtg caccgagtgt      360

gatcctcttc caaacccttc gctgaccgct cggtcgtctc aggccctgag cccacaccct      420

cagcccaccc acttacctta tgtcagtgag atgctggagg ccaggacagc tgggcacatg      480

cagactctgg ctgacttcag gcagctgcct gcccggactc tctctaccca ctggccaccc      540

caaagatccc tgtgcagctc cgattttatt cgcatccttg tgatcttctc tggaatgttc      600

cttgttttca ccctggccgg ggccctgttc ctccatcaac gaaggaaata tagatcaaac      660

aaaggagaaa gtcctgtgga gcctgcagag ccttgtcgtt acagctgccc cagggaggag      720

gagggcagca ccatccccat ccaggaggat taccgaaaac cggagcctgc ctgctccccc      780

tga                                                                    783


<210> 28
<211> 708
<212> DNA
<213> Homo sapiens

<400> 28
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccgagccagt tccgggtgtc gccgctggat cggacctgga acctgggcga gacagtggag      120

ctgaagtgcc aggtgctgct gtccaacccg acgtcgggct gctcgtggct cttccagccg      180

cgcggcgccg ccgccagtcc caccttcctc ctatacctct cccaaaacaa gcccaaggcg      240

gccgaggggc tggacaccca gcggttctcg ggcaagaggt tgggggacac cttcgtcctc      300

accctgagcg acttccgccg agagaacgag ggctactatt tctgctcggc cctgagcaac      360

tccatcatgt acttcagcca cttcgtgccg gtcttcctgc cagcgaagcc caccacgacg      420

ccagcgccgc gaccaccaac accggcgccc accatcgcgt cgcagcccct gtccctgcgc      480

ccagaggcgt gccggccagc ggcggggggc gcagtgcaca cgagggggct ggacttcgcc      540

tgtgatatct acatctgggc gcccttggcc gggacttgtg gggtccttct cctgtcactg      600

gttatcaccc tttactgcaa ccacaggaac cgaagacgtg tttgcaaatg tccccggcct      660

gtggtcaaat cgggagacaa gcccagcctt tcggcgagat acgtctaa                   708


<210> 29
<211> 282
<212> DNA
<213> Homo sapiens

<400> 29
atgatccatc tgggtcacat cctcttcctg cttttgctcc cagtggctgc agctcagacg       60

actccaggag agagatcatc actccctgcc ttttaccctg gcacttcagg ctcttgttcc      120

ggatgtgggt ccctctctct gccgctcctg gcaggcctcg tggctgctga tgcggtggca      180

tcgctgctca tcgtgggggc ggtgttcctg tgcgcacgcc cacgccgcag ccccgcccaa      240

gaagatggca aagtctacat caacatgcca ggcaggggct ga                         282


<210> 30
<211> 549
<212> DNA
<213> Homo sapiens

<400> 30
atggaacagg ggaagggcct ggctgtcctc atcctggcta tcattcttct tcaaggtact       60

ttggcccagt caatcaaagg aaaccacttg gttaaggtgt atgactatca agaagatggt      120

tcggtacttc tgacttgtga tgcagaagcc aaaaatatca catggtttaa agatgggaag      180

atgatcggct tcctaactga agataaaaaa aaatggaatc tgggaagtaa tgccaaggac      240

cctcgaggga tgtatcagtg taaaggatca cagaacaagt caaaaccact ccaagtgtat      300

tacagaatgt gtcagaactg cattgaacta aatgcagcca ccatatctgg ctttctcttt      360

gctgaaatcg tcagcatttt cgtccttgct gttggggtct acttcattgc tggacaggat      420

ggagttcgcc agtcgagagc ttcagacaag cagactctgt tgcccaatga ccagctctac      480

cagcccctca aggatcgaga agatgaccag tacagccacc ttcaaggaaa ccagttgagg      540

aggaattga                                                              549


<210> 31
<211> 624
<212> DNA
<213> Homo sapiens

<400> 31
atgcagtcgg gcactcactg gagagttctg ggcctctgcc tcttatcagt tggcgtttgg       60

gggcaagatg gtaatgaaga aatgggtggt attacacaga caccatataa agtctccatc      120

tctggaacca cagtaatatt gacatgccct cagtatcctg gatctgaaat actatggcaa      180

cacaatgata aaaacatagg cggtgatgag gatgataaaa acataggcag tgatgaggat      240

cacctgtcac tgaaggaatt ttcagaattg gagcaaagtg gttattatgt ctgctacccc      300

agaggaagca aaccagaaga tgcgaacttt tatctctacc tgagggcaag agtgtgtgag      360

aactgcatgg agatggatgt gatgtcggtg gccacaattg tcatagtgga catctgcatc      420

actgggggct tgctgctgct ggtttactac tggagcaaga atagaaaggc caaggccaag      480

cctgtgacac gaggagcggg tgctggcggc aggcaaaggg gacaaaacaa ggagaggcca      540

ccacctgttc ccaacccaga ctatgagccc atccggaaag gccagcggga cctgtattct      600

ggcctgaatc agagacgcat ctga                                             624


<210> 32
<211> 681
<212> DNA
<213> Homo sapiens

<400> 32
atgcctgggg gtccaggagt cctccaagct ctgcctgcca ccatcttcct cctcttcctg       60

ctgtctgctg tctacctggg ccctgggtgc caggccctgt ggatgcacaa ggtcccagca      120

tcattgatgg tgagcctggg ggaagacgcc cacttccaat gcccgcacaa tagcagcaac      180

aacgccaacg tcacctggtg gcgcgtcctc catggcaact acacgtggcc ccctgagttc      240

ttgggcccgg gcgaggaccc caatggtacg ctgatcatcc agaatgtgaa caagagccat      300

gggggcatat acgtgtgccg ggtccaggag ggcaacgagt cataccagca gtcctgcggc      360

acctacctcc gcgtgcgcca gccgcccccc aggcccttcc tggacatggg ggagggcacc      420

aagaaccgaa tcatcacagc cgaggggatc atcctcctgt tctgcgcggt ggtgcctggg      480

acgctgctgc tgttcaggaa acgatggcag aacgagaagc tcgggttgga tgccggggat      540

gaatatgaag atgaaaacct ttatgaaggc ctgaacctgg acgactgctc catgtatgag      600

gacatctccc ggggcctcca gggcacctac caggatgtgg gcagcctcaa cataggagat      660

gtccagctgg agaagccgtg a                                                681


<210> 33
<211> 1280
<212> DNA
<213> Homo sapiens

<400> 33
aggggacagg ctgcagccgg tgcagttaca cgttttcctc caaggagcct cggacgttgt       60

cacgggtttg gggtcgggga cagagcggtg accatggcca ggctggcgtt gtctcctgtg      120

cccagccact ggatggtggc gttgctgctg ctgctctcag ctgagccagt accagcagcc      180

agatcggagg accggtaccg gaatcccaaa ggtagtgctt gttcgcggat ctggcagagc      240

ccacgtttca tagccaggaa acggggcttc acggtgaaaa tgcactgcta catgaacagc      300

gcctccggca atgtgagctg gctctggaag caggagatgg acgagaatcc ccagcagctg      360

aagctggaaa agggccgcat ggaagagtcc cagaacgaat ctctcgccac cctcaccatc      420

caaggcatcc ggtttgagga caatggcatc tacttctgtc agcagaagtg caacaacacc      480

tcggaggtct accagggctg cggcacagag ctgcgagtca tgggattcag caccttggca      540

cagctgaagc agaggaacac gctgaaggat ggtatcatca tgatccagac gctgctgatc      600

atcctcttca tcatcgtgcc tatcttcctg ctgctggaca aggatgacag caaggctggc      660

atggaggaag atcacaccta cgagggcctg gacattgacc agacagccac ctatgaggac      720

atagtgacgc tgcggacagg ggaagtgaag tggtctgtag gtgagcaccc aggccaggag      780

tgagagccag gtcgccccat gacctgggtg caggctccct ggcctcagtg actgcttcgg      840

agctgcctgg ctcatggccc aacccctttc ctggaccccc cagctggcct ctgaagctgg      900

cccaccagag ctgccatttg tctccagccc ctggtcccca gctcttgcca aagggcctgg      960

agtagaagga caacagggca gcaacttgga gggagttctc tggggatgga cgggacccag     1020

ccttctgggg gtgctatgag gtgatccgtc cccacacatg ggatggggga ggcagagact     1080

ggtccagagc ccgcaaatgg actcggagcc gagggcctcc cagcagagct tgggaagggc     1140

catggaccca actgggcccc agaagagcca caggaacatc attcctctcc cgcaaccact     1200

cccaccccag ggaggccctg gcctccagtg ccttcccccg tggaataaac ggtgtgtcct     1260

gagaaaccac acaaaaaaaa                                                 1280


<210> 34
<211> 495
<212> DNA
<213> Homo sapiens

<400> 34
atgaagtgga aggcgctttt caccgcggcc atcctgcagg cacagttgcc gattacagag       60

gcacagagct ttggcctgct ggatcccaaa ctctgctacc tgctggatgg aatcctcttc      120

atctatggtg tcattctcac tgccttgttc ctgagagtga agttcagcag gagcgcagac      180

gcccccgcgt accagcaggg ccagaaccag ctctataacg agctcaatct aggacgaaga      240

gaggagtacg atgttttgga caagagacgt ggccgggacc ctgagatggg gggaaagccg      300

cagagaagga agaaccctca ggaaggcctg tacaatgaac tgcagaaaga taagatggcg      360

gaggcctaca gtgagattgg gatgaaaggc gagcgccgga ggggcaaggg gcacgatggc      420

ctttaccagg gtctcagtac agccaccaag gacacctacg acgcccttca catgcaggcc      480

ctgccccctc gctaa                                                       495


<210> 35
<211> 9
<212> PRT
<213> Homo sapiens

<400> 35
Lys Val Ser Ala Val Thr Leu Ala Tyr 
1               5                   


<210> 36
<211> 9
<212> PRT
<213> Homo sapiens

<400> 36
Arg Pro Lys Ser Asn Ile Val Leu Leu 
1               5                   


<210> 37
<211> 9
<212> PRT
<213> Homo sapiens

<400> 37
Ile Met Ile Gly Val Leu Val Gly Val 
1               5                   


<210> 38
<211> 9
<212> PRT
<213> Homo sapiens

<400> 38
Ile Ile Ser Ala Val Val Gly Ile Leu 
1               5                   


<210> 39
<211> 9
<212> PRT
<213> Homo sapiens

<400> 39
Ser Leu Leu Met Trp Ile Thr Gln Val 
1               5                   


<210> 40
<211> 9
<212> PRT
<213> Homo sapiens

<400> 40
Lys Tyr Ala Asp Lys Ile Tyr Ser Ile 
1               5                   


<210> 41
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      6xHis tag

<400> 41
His His His His His His 
1               5       


<210> 42
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 42
Ala Trp Ile Lys Arg Glu Gln 
1               5           


<210> 43
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (1)..(1)
<223> Any amino acid

<400> 43
Xaa Trp Ile Lys Arg Glu Gln 
1               5           


<210> 44
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (2)..(2)
<223> Any amino acid

<400> 44
Ala Xaa Ile Lys Arg Glu Gln 
1               5           


<210> 45
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (3)..(3)
<223> Any amino acid

<400> 45
Ala Trp Xaa Lys Arg Glu Gln 
1               5           


<210> 46
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (4)..(4)
<223> Any amino acid

<400> 46
Ala Trp Ile Xaa Arg Glu Gln 
1               5           


<210> 47
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (5)..(5)
<223> Any amino acid

<400> 47
Ala Trp Ile Lys Xaa Glu Gln 
1               5           


<210> 48
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (6)..(6)
<223> Any amino acid

<400> 48
Ala Trp Ile Lys Arg Xaa Gln 
1               5           


<210> 49
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (7)..(7)
<223> Any amino acid

<400> 49
Ala Trp Ile Lys Arg Glu Xaa 
1               5           


