                               SEQUENCE LISTING

<110> TWIST BIOSCIENCE CORPORATION
 
<120> DE NOVO SYNTHESIZED COMBINATORIAL NUCLEIC ACID LIBRARIES

<130> 44854-729.601

<140>
<141>

<150> 62/578,326
<151> 2017-10-27

<150> 62/471,723
<151> 2017-03-15

<160> 55    

<170> PatentIn version 3.5

<210> 1
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 1
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 2
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 2
atgtttagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 3
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 3
atgttaagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 4
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 4
atgattagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 5
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 5
atgtctagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 6
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 6
atgcctagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 7
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 7
atgactagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 8
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 8
atggctagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 9
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 9
atgtatagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 10
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 10
atgcatagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 11
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 11
atgcaaagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 12
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 12
atgaatagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 13
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 13
atgaaaagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 14
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 14
atggatagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 15
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 15
atggaaagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 16
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 16
atgtgtagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 17
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 17
atgtggagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 18
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 18
atgcgtagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 19
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 19
atgggtagca agggcgagga gctgttcacc ggggtggtgc ccat                        44


<210> 20
<211> 62
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 20
agacaatcaa ccatttgggg tggacagcct tgacctctag acttcggcat tttttttttt       60

tt                                                                      62


<210> 21
<211> 112
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 21
cgggatcctt atcgtcatcg tcgtacagat cccgacccat ttgctgtcca ccagtcatgc       60

tagccatacc atgatgatga tgatgatgag aaccccgcat tttttttttt tt              112


<210> 22
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 22
atgcggggtt ctcatcatc                                                    19


<210> 23
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 23
cgggatcctt atcgtcatcg                                                   20


<210> 24
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 24
Ala Trp Ile Lys Arg Glu Gln 
1               5           


<210> 25
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (1)..(1)
<223> Any amino acid

<400> 25
Xaa Trp Ile Lys Arg Glu Gln 
1               5           


<210> 26
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (2)..(2)
<223> Any amino acid

<400> 26
Ala Xaa Ile Lys Arg Glu Gln 
1               5           


<210> 27
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (3)..(3)
<223> Any amino acid

<400> 27
Ala Trp Xaa Lys Arg Glu Gln 
1               5           


<210> 28
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (4)..(4)
<223> Any amino acid

<400> 28
Ala Trp Ile Xaa Arg Glu Gln 
1               5           


<210> 29
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (5)..(5)
<223> Any amino acid

<400> 29
Ala Trp Ile Lys Xaa Glu Gln 
1               5           


<210> 30
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (6)..(6)
<223> Any amino acid

<400> 30
Ala Trp Ile Lys Arg Xaa Gln 
1               5           


<210> 31
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (7)..(7)
<223> Any amino acid

<400> 31
Ala Trp Ile Lys Arg Glu Xaa 
1               5           


<210> 32
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      6xHis tag

<400> 32
His His His His His His 
1               5       


<210> 33
<211> 261
<212> PRT
<213> Homo sapiens

<400> 33
Met Phe Cys Gln Leu Ala Lys Thr Cys Pro Val Gln Leu Trp Val Asp 
1               5                   10                  15      


Ser Thr Pro Pro Pro Gly Thr Arg Val Arg Ala Met Ala Ile Tyr Lys 
            20                  25                  30          


Gln Ser Gln His Met Thr Glu Val Val Arg Arg Cys Pro His His Glu 
        35                  40                  45              


Arg Cys Ser Asp Ser Asp Gly Leu Ala Pro Pro Gln His Leu Ile Arg 
    50                  55                  60                  


Val Glu Gly Asn Leu Arg Val Glu Tyr Leu Asp Asp Arg Asn Thr Phe 
65                  70                  75                  80  


Arg His Ser Val Val Val Pro Tyr Glu Pro Pro Glu Val Gly Ser Asp 
                85                  90                  95      


Cys Thr Thr Ile His Tyr Asn Tyr Met Cys Asn Ser Ser Cys Met Gly 
            100                 105                 110         


Gly Met Asn Arg Arg Pro Ile Leu Thr Ile Ile Thr Leu Glu Asp Ser 
        115                 120                 125             


Ser Gly Asn Leu Leu Gly Arg Asn Ser Phe Glu Val Arg Val Cys Ala 
    130                 135                 140                 


Cys Pro Gly Arg Asp Arg Arg Thr Glu Glu Glu Asn Leu Arg Lys Lys 
145                 150                 155                 160 


Gly Glu Pro His His Glu Leu Pro Pro Gly Ser Thr Lys Arg Ala Leu 
                165                 170                 175     


Pro Asn Asn Thr Ser Ser Ser Pro Gln Pro Lys Lys Lys Pro Leu Asp 
            180                 185                 190         


Gly Glu Tyr Phe Thr Leu Gln Ile Arg Gly Arg Glu Arg Phe Glu Met 
        195                 200                 205             


Phe Arg Glu Leu Asn Glu Ala Leu Glu Leu Lys Asp Ala Gln Ala Gly 
    210                 215                 220                 


Lys Glu Pro Gly Gly Ser Arg Ala His Ser Ser His Leu Lys Ser Lys 
225                 230                 235                 240 


Lys Gly Gln Ser Thr Ser Arg His Lys Lys Leu Met Phe Lys Thr Glu 
                245                 250                 255     


Gly Pro Asp Ser Asp 
            260     


<210> 34
<211> 2271
<212> DNA
<213> Homo sapiens

<400> 34
tgaggccagg agatggaggc tgcagtgagc tgtgatcaca ccactgtgct ccagcctgag       60

tgacagagca agaccctatc tcaaaaaaaa aaaaaaaaaa gaaaagctcc tgaggtgtag      120

acgccaactc tctctagctc gctagtgggt tgcaggaggt gcttacgcat gtttgtttct      180

ttgctgccgt cttccagttg ctttatctgt tcacttgtgc cctgactttc aactctgtct      240

ccttcctctt cctacagtac tcccctgccc tcaacaagat gttttgccaa ctggccaaga      300

cctgccctgt gcagctgtgg gttgattcca cacccccgcc cggcacccgc gtccgcgcca      360

tggccatcta caagcagtca cagcacatga cggaggttgt gaggcgctgc ccccaccatg      420

agcgctgctc agatagcgat ggtctggccc ctcctcagca tcttatccga gtggaaggaa      480

atttgcgtgt ggagtatttg gatgacagaa acacttttcg acatagtgtg gtggtgccct      540

atgagccgcc tgaggttggc tctgactgta ccaccatcca ctacaactac atgtgtaaca      600

gttcctgcat gggcggcatg aaccggaggc ccatcctcac catcatcaca ctggaagact      660

ccagtggtaa tctactggga cggaacagct ttgaggtgcg tgtttgtgcc tgtcctggga      720

gagaccggcg cacagaggaa gagaatctcc gcaagaaagg ggagcctcac cacgagctgc      780

ccccagggag cactaagcga gcactgccca acaacaccag ctcctctccc cagccaaaga      840

agaaaccact ggatggagaa tatttcaccc ttcagatccg tgggcgtgag cgcttcgaga      900

tgttccgaga gctgaatgag gccttggaac tcaaggatgc ccaggctggg aaggagccag      960

gggggagcag ggctcactcc agccacctga agtccaaaaa gggtcagtct acctcccgcc     1020

ataaaaaact catgttcaag acagaagggc ctgactcaga ctgacattct ccacttcttg     1080

ttccccactg acagcctccc acccccatct ctccctcccc tgccattttg ggttttgggt     1140

ctttgaaccc ttgcttgcaa taggtgtgcg tcagaagcac ccaggacttc catttgcttt     1200

gtcccggggc tccactgaac aagttggcct gcactggtgt tttgttgtgg ggaggaggat     1260

ggggagtagg acataccagc ttagatttta aggtttttac tgtgagggat gtttgggaga     1320

tgtaagaaat gttcttgcag ttaagggtta gtttacaatc agccacattc taggtagggg     1380

cccacttcac cgtactaacc agggaagctg tccctcactg ttgaattttc tctaacttca     1440

aggcccatat ctgtgaaatg ctggcatttg cacctacctc acagagtgca ttgtgagggt     1500

taatgaaata atgtacatct ggccttgaaa ccacctttta ttacatgggg tctagaactt     1560

gacccccttg agggtgcttg ttccctctcc ctgttggtcg gtgggttggt agtttctaca     1620

gttgggcagc tggttaggta gagggagttg tcaagtctct gctggcccag ccaaaccctg     1680

tctgacaacc tcttggtgaa ccttagtacc taaaaggaaa tctcacccca tcccacaccc     1740

tggaggattt catctcttgt atatgatgat ctggatccac caagacttgt tttatgctca     1800

gggtcaattt cttttttctt tttttttttt ttttttcttt ttctttgaga ctgggtctcg     1860

ctttgttgcc caggctggag tggagtggcg tgatcttggc ttactgcagc ctttgcctcc     1920

ccggctcgag cagtcctgcc tcagcctccg gagtagctgg gaccacaggt tcatgccacc     1980

atggccagcc aacttttgca tgttttgtag agatggggtc tcacagtgtt gcccaggctg     2040

gtctcaaact cctgggctca ggcgatccac ctgtctcagc ctcccagagt gctgggatta     2100

caattgtgag ccaccacgtc cagctggaag ggtcaacatc ttttacattc tgcaagcaca     2160

tctgcatttt caccccaccc ttcccctcct tctccctttt tatatcccat ttttatatcg     2220

atctcttatt ttacaataaa actttgctgc cacctgtgtg tctgaggggt g              2271


<210> 35
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 35
cccctgccct caacaagatg gcttgccaac tggccaa                                37


<210> 36
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 36
cccctgccct caacaagatg tgctgccaac tggccaa                                37


<210> 37
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 37
cccctgccct caacaagatg gattgccaac tggccaa                                37


<210> 38
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 38
cccctgccct caacaagatg gagtgccaac tggccaa                                37


<210> 39
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 39
cccctgccct caacaagatg ttctgccaac tggccaa                                37


<210> 40
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 40
cccctgccct caacaagatg ggttgccaac tggccaa                                37


<210> 41
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 41
cccctgccct caacaagatg cactgccaac tggccaa                                37


<210> 42
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 42
cccctgccct caacaagatg atctgccaac tggccaa                                37


<210> 43
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 43
cccctgccct caacaagatg aagtgccaac tggccaa                                37


<210> 44
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 44
cccctgccct caacaagatg ctgtgccaac tggccaa                                37


<210> 45
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 45
cccctgccct caacaagatg atgtgccaac tggccaa                                37


<210> 46
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 46
cccctgccct caacaagatg aactgccaac tggccaa                                37


<210> 47
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 47
cccctgccct caacaagatg ccttgccaac tggccaa                                37


<210> 48
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 48
cccctgccct caacaagatg cagtgccaac tggccaa                                37


<210> 49
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 49
cccctgccct caacaagatg agatgccaac tggccaa                                37


<210> 50
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 50
cccctgccct caacaagatg agctgccaac tggccaa                                37


<210> 51
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 51
cccctgccct caacaagatg acctgccaac tggccaa                                37


<210> 52
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 52
cccctgccct caacaagatg gtgtgccaac tggccaa                                37


<210> 53
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 53
cccctgccct caacaagatg tggtgccaac tggccaa                                37


<210> 54
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 54
cccctgccct caacaagatg tactgccaac tggccaa                                37


<210> 55
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 55
His His His Cys Cys His His Cys His His 
1               5                   10  


