                         SEQUENCE LISTING

<110>  Oxford BioMedica (UK) Limited
 
<120>  METHOD

<130>  P111548PCT

<150>  GB 1704084.1
<151>  2017-03-15

<150>  GB 1721603.7
<151>  2017-12-21

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  515
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  5T4-specific CAR sequence

<400>  1

Met Gly Val Leu Leu Thr Gln Arg Thr Leu Leu Ser Leu Val Leu Ala 
1               5                   10                  15      


Leu Leu Phe Pro Ser Met Ala Ser Met Glu Val Gln Leu Gln Gln Ser 
            20                  25                  30          


Gly Pro Asp Leu Val Lys Pro Gly Ala Ser Val Lys Ile Ser Cys Lys 
        35                  40                  45              


Ala Ser Gly Tyr Ser Phe Thr Gly Tyr Tyr Met His Trp Val Lys Gln 
    50                  55                  60                  


Ser His Gly Lys Ser Leu Glu Trp Ile Gly Arg Ile Asn Pro Asn Asn 
65                  70                  75                  80  


Gly Val Thr Leu Tyr Asn Gln Lys Phe Lys Asp Lys Ala Ile Leu Thr 
                85                  90                  95      


Val Asp Lys Ser Ser Thr Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr 
            100                 105                 110         


Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg Ser Thr Met Ile Thr 
        115                 120                 125             


Asn Tyr Val Met Asp Tyr Trp Gly Gln Val Thr Ser Val Thr Val Ser 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Thr Gly Gly Gly Gly Ser 
145                 150                 155                 160 


Ser Ile Val Met Thr Gln Thr Pro Thr Phe Leu Leu Val Ser Ala Gly 
                165                 170                 175     


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Ser Val Ser Asn Asp 
            180                 185                 190         


Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Thr Leu Leu Ile 
        195                 200                 205             


Ser Tyr Thr Ser Ser Arg Tyr Ala Gly Val Pro Asp Arg Phe Ile Gly 
    210                 215                 220                 


Ser Gly Tyr Gly Thr Asp Phe Thr Phe Thr Ile Ser Thr Leu Gln Ala 
225                 230                 235                 240 


Glu Asp Leu Ala Val Tyr Phe Cys Gln Gln Asp Tyr Asn Ser Pro Pro 
                245                 250                 255     


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala Ala Ala Leu 
            260                 265                 270         


Ser Asn Ser Ile Met Tyr Phe Ser His Phe Val Pro Val Phe Leu Pro 
        275                 280                 285             


Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro 
    290                 295                 300                 


Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro 
305                 310                 315                 320 


Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
                325                 330                 335     


Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
            340                 345                 350         


Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu 
        355                 360                 365             


Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu 
    370                 375                 380                 


Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys 
385                 390                 395                 400 


Glu Leu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
                405                 410                 415     


Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
            420                 425                 430         


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
        435                 440                 445             


Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
    450                 455                 460                 


Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys 
465                 470                 475                 480 


Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu 
                485                 490                 495     


Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu 
            500                 505                 510         


Pro Pro Arg 
        515 


<210>  2
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Oncostatin M leader

<400>  2

Met Gly Val Leu Leu Thr Gln Arg Thr Leu Leu Ser Leu Val Leu Ala 
1               5                   10                  15      


Leu Leu Phe Pro Ser Met Ala Ser 
            20                  


<210>  3
<211>  244
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  H8 anti5T4 domain

<400>  3

Met Glu Val Gln Leu Gln Gln Ser Gly Pro Asp Leu Val Lys Pro Gly 
1               5                   10                  15      


Ala Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Gly 
            20                  25                  30          


Tyr Tyr Met His Trp Val Lys Gln Ser His Gly Lys Ser Leu Glu Trp 
        35                  40                  45              


Ile Gly Arg Ile Asn Pro Asn Asn Gly Val Thr Leu Tyr Asn Gln Lys 
    50                  55                  60                  


Phe Lys Asp Lys Ala Ile Leu Thr Val Asp Lys Ser Ser Thr Thr Ala 
65                  70                  75                  80  


Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr 
                85                  90                  95      


Cys Ala Arg Ser Thr Met Ile Thr Asn Tyr Val Met Asp Tyr Trp Gly 
            100                 105                 110         


Gln Val Thr Ser Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             


Gly Gly Thr Gly Gly Gly Gly Ser Ser Ile Val Met Thr Gln Thr Pro 
    130                 135                 140                 


Thr Phe Leu Leu Val Ser Ala Gly Asp Arg Val Thr Ile Thr Cys Lys 
145                 150                 155                 160 


Ala Ser Gln Ser Val Ser Asn Asp Val Ala Trp Tyr Gln Gln Lys Pro 
                165                 170                 175     


Gly Gln Ser Pro Thr Leu Leu Ile Ser Tyr Thr Ser Ser Arg Tyr Ala 
            180                 185                 190         


Gly Val Pro Asp Arg Phe Ile Gly Ser Gly Tyr Gly Thr Asp Phe Thr 
        195                 200                 205             


Phe Thr Ile Ser Thr Leu Gln Ala Glu Asp Leu Ala Val Tyr Phe Cys 
    210                 215                 220                 


Gln Gln Asp Tyr Asn Ser Pro Pro Thr Phe Gly Gly Gly Thr Lys Leu 
225                 230                 235                 240 


Glu Ile Lys Arg 
                


<210>  4
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  4

Ala Ala Ala 
1           


<210>  5
<211>  65
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8 hinge

<400>  5

Leu Ser Asn Ser Ile Met Tyr Phe Ser His Phe Val Pro Val Phe Leu 
1               5                   10                  15      


Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala 
            20                  25                  30          


Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg 
        35                  40                  45              


Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys 
    50                  55                  60                  


Asp 
65  


<210>  6
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8 transmembrane region

<400>  6

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr Leu Tyr Cys 
            20                  


<210>  7
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  41BB costimulatory domain

<400>  7

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  8
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3 zeta stimulatory domain

<400>  8

Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln 
1               5                   10                  15      


Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
            20                  25                  30          


Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly 
        35                  40                  45              


Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
    50                  55                  60                  


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
65                  70                  75                  80  


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
                85                  90                  95      


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
            100                 105                 110         


Arg 
    


<210>  9
<211>  1548
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon-optimised sequence encoding the 5T4-CAR protein

<400>  9
atgggagtgc tgctgaccca gagaaccctg ctgtctctgg tgctggccct gctgttccct       60

agcatggcca gcatggaagt gcagctgcag cagagcggcc ctgacctcgt gaaacctggc      120

gcctccgtga agatcagctg caaggccagc ggctacagct tcaccggcta ctacatgcac      180

tgggtcaagc agagccacgg caagagcctg gaatggatcg gccggatcaa ccccaacaac      240

ggcgtgaccc tgtacaacca gaagttcaag gacaaggcca tcctgaccgt ggacaagagc      300

agcaccaccg cctacatgga actgcggagc ctgaccagcg aggacagcgc cgtgtactac      360

tgcgcccggt ccaccatgat caccaactac gtgatggact actggggcca agtgaccagc      420

gtgaccgtgt ctagcggagg cggaggatct ggcggcggag gaacaggcgg agggggatct      480

agcatcgtga tgacccagac ccccaccttc ctgctggtgt ctgccggcga cagagtgacc      540

atcacatgca aggcctccca gagcgtgtcc aacgacgtgg cctggtatca gcagaagcct      600

ggccagagcc ccaccctgct gattagctac accagctcca gatatgccgg cgtgcccgac      660

agattcatcg gcagcggcta tggcaccgac ttcaccttca ccatcagcac actgcaggcc      720

gaggacctgg ctgtgtactt ctgtcagcaa gactacaaca gcccccctac cttcggcgga      780

ggcaccaagc tggaaatcaa gagagccgcc gctctgagca acagcatcat gtacttcagc      840

cacttcgtgc ccgtgtttct gcccgccaag cctaccacaa cccctgcccc tagacctcct      900

accccagccc ctacaatcgc cagccagcct ctgtctctga ggcccgaggc ttgtagacct      960

gctgctggcg gagccgtgca caccagagga ctggatttcg cctgcgacat ctacatctgg     1020

gcccctctgg ccggcacatg tggcgtgctg ctgctgagcc tcgtgatcac cctgtactgc     1080

aagcggggca gaaagaagct gctgtacatc ttcaagcagc ccttcatgcg gcccgtgcag     1140

accacccagg aagaggacgg ctgctcctgc agattccccg aggaagaaga aggcggctgc     1200

gagctgctga gagtgaagtt cagcagatcc gccgacgccc ctgcctacca gcagggacag     1260

aatcagctgt acaacgagct gaacctgggc agacgggaag agtacgacgt gctggacaag     1320

cggagaggca gggaccctga gatgggcggc aagcccagaa gaaagaaccc ccaggaaggc     1380

ctgtataacg aactgcagaa agacaagatg gccgaggcct acagcgagat cggaatgaag     1440

ggcgagcgga gaagaggcaa gggccacgat ggactgtacc agggcctgag caccgccacc     1500

aaggacacct atgacgccct gcacatgcag gctctgcccc ccagatga                  1548


<210>  10
<211>  1179
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EF1alpha promoter sequence

<400>  10
ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg       60

ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt      120

gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca      180

gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc      240

gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt      300

acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg      360

gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg      420

cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct      480

ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg      540

caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc      600

gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga      660

gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct      720

ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca      780

gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg      840

acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg      900

tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat      960

tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg     1020

gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa     1080

ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca     1140

gtggttcaaa gtttttttct tccatttcag gtgtcgtga                            1179


<210>  11
<211>  243
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2E4 anti5T4 domain

<400>  11

Met Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly 
1               5                   10                  15      


Ala Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Gly 
            20                  25                  30          


Tyr Tyr Met His Trp Val Lys Gln Ser His Val Lys Ser Leu Glu Trp 
        35                  40                  45              


Ile Gly Arg Ile Asn Pro Tyr Asn Gly Ala Thr Thr Tyr Asn Gln Asp 
    50                  55                  60                  


Phe Lys Asp Lys Ala Ser Leu Thr Val Asp Lys Ser Ser Ser Thr Ala 
65                  70                  75                  80  


Ser Met Glu Leu His Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr 
                85                  90                  95      


Cys Ala Leu Ser Thr Met Ile Thr Thr Ala Trp Phe Ala Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Leu Val Thr Val Ser Pro Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             


Gly Gly Thr Gly Gly Gly Gly Ser Asn Phe Val Met Thr Gln Thr Pro 
    130                 135                 140                 


Lys Phe Leu Leu Ala Ser Ala Gly Asp Arg Val Thr Ile Ser Cys Lys 
145                 150                 155                 160 


Ala Ser Gln Ser Val Ser Asn Asp Val Gly Trp Tyr Gln Gln Lys Pro 
                165                 170                 175     


Gly Gln Ser Pro Lys Leu Leu Ile Tyr Phe Ala Ser Asn Arg Tyr Thr 
            180                 185                 190         


Gly Val Pro Asp Arg Phe Ile Gly Ser Gly Tyr Gly Thr Asp Phe Thr 
        195                 200                 205             


Phe Thr Ile Ser Thr Val Gln Ala Glu Asp Leu Ala Val Tyr Phe Cys 
    210                 215                 220                 


Gln Gln Asp Tyr Ser Ser Pro Phe Thr Phe Gly Ser Gly Thr Lys Leu 
225                 230                 235                 240 


Glu Ile Lys 
            


<210>  12
<211>  243
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2E4 antibody sequence

<400>  12

Met Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly 
1               5                   10                  15      


Ala Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Gly 
            20                  25                  30          


Tyr Tyr Met His Trp Val Lys Gln Ser His Val Lys Ser Leu Glu Trp 
        35                  40                  45              


Ile Gly Arg Ile Asn Pro Tyr Asn Gly Ala Thr Thr Tyr Asn Gln Asp 
    50                  55                  60                  


Phe Lys Asp Lys Ala Ser Leu Thr Val Asp Lys Ser Ser Ser Thr Ala 
65                  70                  75                  80  


Ser Met Glu Leu His Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr 
                85                  90                  95      


Cys Ala Leu Ser Thr Met Ile Thr Thr Ala Trp Phe Ala Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Leu Val Thr Val Ser Pro Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             


Gly Gly Thr Gly Gly Gly Gly Ser Asn Phe Val Met Thr Gln Thr Pro 
    130                 135                 140                 


Lys Phe Leu Leu Ala Ser Ala Gly Asp Arg Val Thr Ile Ser Cys Lys 
145                 150                 155                 160 


Ala Ser Gln Ser Val Ser Asn Asp Val Gly Trp Tyr Gln Gln Lys Pro 
                165                 170                 175     


Gly Gln Ser Pro Lys Leu Leu Ile Tyr Phe Ala Ser Asn Arg Tyr Thr 
            180                 185                 190         


Gly Val Pro Asp Arg Phe Ile Gly Ser Gly Tyr Gly Thr Asp Phe Thr 
        195                 200                 205             


Phe Thr Ile Ser Thr Val Gln Ala Glu Asp Leu Ala Val Tyr Phe Cys 
    210                 215                 220                 


Gln Gln Asp Tyr Ser Ser Pro Phe Thr Phe Gly Ser Gly Thr Lys Leu 
225                 230                 235                 240 


Glu Ile Lys 
            


<210>  13
<211>  514
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  5T4-specific CAR sequence

<400>  13

Met Gly Val Leu Leu Thr Gln Arg Thr Leu Leu Ser Leu Val Leu Ala 
1               5                   10                  15      


Leu Leu Phe Pro Ser Met Ala Ser Met Glu Val Gln Leu Gln Gln Ser 
            20                  25                  30          


Gly Pro Glu Leu Val Lys Pro Gly Ala Ser Val Lys Ile Ser Cys Lys 
        35                  40                  45              


Ala Ser Gly Tyr Ser Phe Thr Gly Tyr Tyr Met His Trp Val Lys Gln 
    50                  55                  60                  


Ser His Val Lys Ser Leu Glu Trp Ile Gly Arg Ile Asn Pro Tyr Asn 
65                  70                  75                  80  


Gly Ala Thr Thr Tyr Asn Gln Asp Phe Lys Asp Lys Ala Ser Leu Thr 
                85                  90                  95      


Val Asp Lys Ser Ser Ser Thr Ala Ser Met Glu Leu His Ser Leu Thr 
            100                 105                 110         


Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Leu Ser Thr Met Ile Thr 
        115                 120                 125             


Thr Ala Trp Phe Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser 
    130                 135                 140                 


Pro Gly Gly Gly Gly Ser Gly Gly Gly Gly Thr Gly Gly Gly Gly Ser 
145                 150                 155                 160 


Asn Phe Val Met Thr Gln Thr Pro Lys Phe Leu Leu Ala Ser Ala Gly 
                165                 170                 175     


Asp Arg Val Thr Ile Ser Cys Lys Ala Ser Gln Ser Val Ser Asn Asp 
            180                 185                 190         


Val Gly Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile 
        195                 200                 205             


Tyr Phe Ala Ser Asn Arg Tyr Thr Gly Val Pro Asp Arg Phe Ile Gly 
    210                 215                 220                 


Ser Gly Tyr Gly Thr Asp Phe Thr Phe Thr Ile Ser Thr Val Gln Ala 
225                 230                 235                 240 


Glu Asp Leu Ala Val Tyr Phe Cys Gln Gln Asp Tyr Ser Ser Pro Phe 
                245                 250                 255     


Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys Ala Ala Ala Leu Ser 
            260                 265                 270         


Asn Ser Ile Met Tyr Phe Ser His Phe Val Pro Val Phe Leu Pro Ala 
        275                 280                 285             


Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr 
    290                 295                 300                 


Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala 
305                 310                 315                 320 


Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile 
                325                 330                 335     


Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser 
            340                 345                 350         


Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr 
        355                 360                 365             


Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu 
    370                 375                 380                 


Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu 
385                 390                 395                 400 


Leu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln 
                405                 410                 415     


Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu 
            420                 425                 430         


Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly 
        435                 440                 445             


Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu 
    450                 455                 460                 


Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly 
465                 470                 475                 480 


Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser 
                485                 490                 495     


Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro 
            500                 505                 510         


Pro Arg 
        


<210>  14
<211>  730
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence encoding the 2E4 antigen binding moiety

<400>  14
atggaagtgc agctgcagca gtctggcccc gagctcgtga aacctggcgc ctccgtgaag       60

atcagctgca aggccagcgg ctacagcttc accggctact acatgcactg ggtcaagcag      120

agccacgtga agtccctgga atggatcggc cggatcaacc cctacaacgg cgccaccacc      180

tacaaccagg acttcaagga caaggcctcc ctgaccgtgg acaagagcag cagcaccgcc      240

agcatggaac tgcacagcct gaccagcgag gacagcgccg tgtactactg tgccctgagc      300

accatgatca ccaccgcttg gtttgcctac tggggccagg gcacactcgt gaccgtgtct      360

ccaggaggcg gaggatctgg cggcggagga acaggcggag ggggatctaa cttcgtgatg      420

acccagaccc ccaagttcct gctggcctct gccggcgaca gagtgaccat cagctgcaag      480

gccagccaga gcgtgtccaa cgacgtgggc tggtatcagc agaagcccgg ccagagcccc      540

aagctgctga tctacttcgc cagcaaccgg tacaccggcg tgcccgacag attcatcggc      600

agcggctacg gcaccgactt caccttcacc atcagcaccg tgcaggccga ggacctggcc      660

gtgtacttct gtcagcaaga ctacagcagc cctttcacct tcggctccgg caccaagctg      720

gaaatcaagg                                                             730


